FastQCFastQC Report
Fri 27 May 2016
SRR939324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212536
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93650.4232699490539363No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86000.38869424045529655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72680.3284918301894297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50860.2298719659250742No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49460.2235443852664996No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33650.1520879208293108No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32820.14833656943887014No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24280.10973832742156513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122250.025.1431061
TACATGG122300.024.781062
ACATGGG125500.023.582313
TACCTGG18400.023.4568162
GAGTACT84250.022.58791712-13
TATATCG1050.002216284622.5848065
GTACACG3151.4551915E-1121.1145111
AGTACTT87500.020.936412-13
GTACTTT89350.020.92728814-15
GTGTAGC12450.020.6057281
TATCGCG600.004738734619.8110290-91
GTACCTG21650.019.7491381
CATGGGG48000.019.465724
GTATCAA219800.019.3445631
CATGGGA75850.018.6340034
CATGGGC22200.018.3734594
AGAGTAC170100.018.0275210-11
ACTTTTT107150.017.82760616-17
TAGACCG1600.001203472317.7859364
TACTTTT101700.017.71018814-15
Produced by FastQC (version 0.11.5)