FastQCFastQC Report
Fri 27 May 2016
SRR939324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212536
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96830.43764259654984144No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96180.4347047912440747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79360.35868342933177133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.24984904200428829No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45680.20645991748834822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31150.14078866965328474No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30220.13658534821580304No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25500.11525236199546583No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22340.1009701085089689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118600.026.154731
TACATGG118350.025.420732
ACATGGG118600.024.0067623
TACCTGG18800.022.2122152
GAGTACT86400.021.50376512-13
CATGGGG46300.020.6007064
AGTACTT89700.020.52748712-13
GTACCTG21250.020.3736951
GTACTTT92400.020.08173614-15
GTGTAGC11650.020.010481
CATGGGA69800.019.6475964
GTATCAA220000.018.5113641
GTACTAG4900.018.4478131
AGAGTAC168450.017.98814410-11
ATTCGCG2550.017.69610280-81
TACTTTT104400.017.63712114-15
GTGTACG1901.8439973E-417.5279771
CCTATAC5500.017.255773
CGCGGAA2500.017.16662282-83
ACTTTTT109450.017.10402316-17
Produced by FastQC (version 0.11.5)