FastQCFastQC Report
Fri 27 May 2016
SRR939323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149509
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75050.2382911114081592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64460.20466682266981934No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40520.12865497447379892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38100.12097123710394224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG110000.025.6667231
GGTATCA110750.025.4072171
GTATCAA150100.025.1323181
TACATGG111900.024.6611962
ACATGGG112750.023.3818843
GAGTACT81850.021.70971312-13
ATCAACG173150.021.4417653
TCAACGC173550.021.3920064
CAACGCA174850.021.2322835
TATCAAC176850.021.0199782
AACGCAG178400.020.8772186
CATGGGG67750.020.8555344
GTACTTT86750.020.24103514-15
GTATACG2601.5103069E-820.0754591
ATTCGCG3450.019.97724580-81
ACGCAGA196900.018.7953437
AGAGTAC145950.018.69915210-11
CGCAGAG197900.018.6524838
CGCGGAA3700.018.62743282-83
AGTACTT88800.018.463112-13
Produced by FastQC (version 0.11.5)