##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3149509 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82550295935017 33.0 31.0 34.0 30.0 34.0 2 31.89365993238946 33.0 31.0 34.0 30.0 34.0 3 31.942564063160322 34.0 31.0 34.0 30.0 34.0 4 35.42178415746709 37.0 35.0 37.0 33.0 37.0 5 35.45055880138777 37.0 35.0 37.0 33.0 37.0 6 35.53194005795824 37.0 35.0 37.0 33.0 37.0 7 35.46482038946388 37.0 35.0 37.0 33.0 37.0 8 35.4483009891383 37.0 35.0 37.0 33.0 37.0 9 37.068775482146584 39.0 37.0 39.0 33.0 39.0 10-11 36.9597316597603 39.0 37.0 39.0 33.0 39.0 12-13 36.852410804350775 39.0 37.0 39.0 33.0 39.0 14-15 38.085725743282524 40.0 38.0 41.0 33.0 41.0 16-17 38.00370883207509 40.0 38.0 41.0 33.0 41.0 18-19 37.91327886346729 40.0 38.0 41.0 33.0 41.0 20-21 37.878519794672755 40.0 38.0 41.0 32.5 41.0 22-23 37.88653802862605 40.0 38.0 41.0 32.5 41.0 24-25 37.801564466080265 40.0 38.0 41.0 32.0 41.0 26-27 37.70949360678125 40.0 38.0 41.0 32.0 41.0 28-29 37.56446401645463 40.0 37.5 41.0 32.0 41.0 30-31 37.393249868471564 40.0 37.0 41.0 31.5 41.0 32-33 37.18494835226697 40.0 37.0 41.0 31.0 41.0 34-35 36.90105314828438 40.0 36.5 41.0 30.0 41.0 36-37 36.66756770658537 40.0 36.0 41.0 30.0 41.0 38-39 36.077953738185855 38.5 35.5 40.5 28.5 41.0 40-41 36.058025870064185 39.0 35.0 40.0 30.0 41.0 42-43 36.26487731897258 39.0 36.0 41.0 30.0 41.0 44-45 36.182611003810436 40.0 36.0 41.0 29.0 41.0 46-47 35.92913006440051 39.0 35.0 41.0 28.0 41.0 48-49 35.69481385828712 39.0 35.0 41.0 27.0 41.0 50-51 34.56516999951421 38.0 34.0 40.0 25.0 40.5 52-53 34.67099395493075 38.0 34.5 39.5 25.5 40.5 54-55 35.16039404872315 39.0 35.0 40.0 26.0 41.0 56-57 35.069341602135445 39.0 35.0 41.0 26.0 41.0 58-59 34.85077102494389 38.5 34.5 40.0 26.0 41.0 60-61 34.54191319980353 38.0 34.0 40.0 25.0 41.0 62-63 34.15370586335838 37.5 34.0 40.0 23.5 41.0 64-65 33.75652363590642 37.0 33.5 40.0 22.5 41.0 66-67 33.39060802810851 36.5 33.0 39.5 22.0 41.0 68-69 32.983955435593295 36.0 33.0 39.0 21.0 41.0 70-71 32.56842939645513 35.5 32.0 39.0 20.0 40.0 72-73 32.12473102950333 35.0 32.0 38.0 18.5 40.0 74-75 31.664289576565743 35.0 31.5 37.0 17.5 39.0 76-77 31.20312388375458 35.0 31.0 37.0 13.5 39.0 78-79 30.804721624862797 35.0 31.0 36.0 9.0 39.0 80-81 30.407175372415193 34.5 30.5 36.0 7.0 37.5 82-83 29.983898760092444 34.0 30.0 35.0 3.5 37.0 84-85 29.56889105571694 34.0 30.0 35.0 2.0 36.5 86-87 29.14170986652205 34.0 29.0 35.0 2.0 36.0 88-89 28.717168930141174 34.0 29.0 35.0 2.0 36.0 90-91 28.31434947479115 34.0 29.0 35.0 2.0 35.5 92-93 27.87114181924865 34.0 27.5 35.0 2.0 35.0 94-95 27.387904749597478 34.0 27.0 35.0 2.0 35.0 96-97 26.77293254281858 33.0 25.5 35.0 2.0 35.0 98-99 25.971647485369942 33.0 24.5 35.0 2.0 35.0 100-101 23.302842284305267 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30362688219844003 1101 2 0.31425028345443806 1101 3 0.3743993042777909 1101 4 0.2584732834307957 1101 5 0.23262088369433798 1101 6 0.36793299077613995 1101 7 0.3355889174004574 1101 8 0.15174562415479897 1101 9 0.30088330945202557 1101 10-11 0.4654539219945377 1101 12-13 0.43133913537261037 1101 14-15 0.45867958568332057 1101 16-17 0.5268257004727843 1101 18-19 0.5579021958731332 1101 20-21 0.5889813694469126 1101 22-23 0.5975173845214101 1101 24-25 0.6581287194494223 1101 26-27 0.5896731469648486 1101 28-29 0.6438737389680256 1101 30-31 0.6585998400854791 1101 32-33 0.5239560384027158 1101 34-35 0.5139508501632335 1101 36-37 0.4508557956722399 1101 38-39 0.9079698275020434 1101 40-41 0.694041405045347 1101 42-43 0.6420106470103661 1101 44-45 0.513620782162171 1101 46-47 0.5591266130987265 1101 48-49 0.4545673354717721 1101 50-51 0.46995317060429187 1101 52-53 0.44147286935956487 1101 54-55 0.4432704699157739 1101 56-57 0.4524047602758756 1101 58-59 0.424195322144854 1101 60-61 0.40888246348150403 1101 62-63 0.4548164986939298 1101 64-65 0.2705664800382763 1101 66-67 0.24334172792438835 1101 68-69 0.25546299615501766 1101 70-71 0.3971214988218037 1101 72-73 0.26006018951679977 1101 74-75 0.21968562595446528 1101 76-77 0.27509075395290594 1101 78-79 0.34909698163698266 1101 80-81 0.36576483585879416 1101 82-83 0.2196859195622416 1101 84-85 0.2637712428908472 1101 86-87 0.29202821707946214 1101 88-89 0.2424974683044958 1101 90-91 0.2612001992035573 1101 92-93 0.3904446765762266 1101 94-95 0.40249750355287617 1101 96-97 0.38386931774966015 1101 98-99 0.41155591218668164 1101 100-101 0.08051798984323355 1102 1 0.32576473653484683 1102 2 0.24058725795432068 1102 3 0.3671593045367736 1102 4 0.2536310947067051 1102 5 0.2794597524873339 1102 6 0.31126730524722745 1102 7 0.3055865218306195 1102 8 0.28195654884973465 1102 9 0.41213038533779667 1102 10-11 0.45162523362629514 1102 12-13 0.33296463658707864 1102 14-15 0.5865691484196134 1102 16-17 0.5302568188751948 1102 18-19 0.5457758064052811 1102 20-21 0.5807155329124285 1102 22-23 0.5590824337052993 1102 24-25 0.48702389857679407 1102 26-27 0.5003253645104806 1102 28-29 0.44751097100411386 1102 30-31 0.5009277662043061 1102 32-33 0.4040164306685625 1102 34-35 0.3604299352448166 1102 36-37 0.38290316619816167 1102 38-39 0.7325772603426728 1102 40-41 0.6723781823216015 1102 42-43 0.4133210087712982 1102 44-45 0.38233483927634637 1102 46-47 0.40599055817892094 1102 48-49 0.3867007423157247 1102 50-51 0.31097585978832143 1102 52-53 0.3569158530542538 1102 54-55 0.3749619912420741 1102 56-57 0.45825908970806495 1102 58-59 0.4976283779207904 1102 60-61 0.5010084345962156 1102 62-63 0.6325946749032809 1102 64-65 0.4728274049075267 1102 66-67 0.40622142698902053 1102 68-69 0.3943492703367042 1102 70-71 0.45889644673933105 1102 72-73 0.4630528588738727 1102 74-75 0.28931204156939216 1102 76-77 0.4075060013316012 1102 78-79 0.29846180700193514 1102 80-81 0.25974256751811353 1102 82-83 0.19927339845159509 1102 84-85 0.22301044776087764 1102 86-87 0.11134597273432689 1102 88-89 0.16211016293209113 1102 90-91 0.18762395708067459 1102 92-93 0.21517064281014697 1102 94-95 0.13949319501343993 1102 96-97 0.15052176538952722 1102 98-99 0.3604752412478369 1102 100-101 0.21182273371896443 1103 1 0.26463441533372034 1103 2 0.31194401050532505 1103 3 0.3529804993473995 1103 4 0.3314138762732455 1103 5 0.3510471252034577 1103 6 0.31510350201106263 1103 7 0.2150472991865371 1103 8 0.2863789568066508 1103 9 0.42689478243946866 1103 10-11 0.4595188961792047 1103 12-13 0.5044292430799686 1103 14-15 0.5548197990979347 1103 16-17 0.5141825190526319 1103 18-19 0.5641267681854956 1103 20-21 0.6067860774163023 1103 22-23 0.6213679612867011 1103 24-25 0.5872640308948007 1103 26-27 0.5682663500240182 1103 28-29 0.483277347079472 1103 30-31 0.45477413155867197 1103 32-33 0.4501732236462175 1103 34-35 0.2825182194183995 1103 36-37 0.22953561636278152 1103 38-39 0.6066680013269945 1103 40-41 0.5725919828205477 1103 42-43 0.4494059428835371 1103 44-45 0.41815812725602797 1103 46-47 0.5146073920637804 1103 48-49 0.3593596194209425 1103 50-51 0.2943657582287571 1103 52-53 0.5073171098474489 1103 54-55 0.48674037740128995 1103 56-57 0.5248657109096158 1103 58-59 0.5275527067628474 1103 60-61 0.531263459378593 1103 62-63 0.3630240396128457 1103 64-65 0.2957602889898041 1103 66-67 0.32065249001421847 1103 68-69 0.31069161965515946 1103 70-71 0.24448892876965544 1103 72-73 0.2851770241762708 1103 74-75 0.19331478770811827 1103 76-77 0.2176204543295519 1103 78-79 0.22546259794735946 1103 80-81 0.3025600746355117 1103 82-83 0.18868282999889985 1103 84-85 0.27534041740868886 1103 86-87 0.1880975279341648 1103 88-89 0.21910356461916436 1103 90-91 0.17772970794500154 1103 92-93 0.19535074700289812 1103 94-95 0.2966446123861495 1103 96-97 0.40456635013822506 1103 98-99 0.4629277541777981 1103 100-101 0.11511485515447717 1104 1 0.25490357225506344 1104 2 0.3938276632618134 1104 3 0.29879244189698895 1104 4 0.21200585154263507 1104 5 0.2868915914505408 1104 6 0.2356141115822865 1104 7 0.31383673780957366 1104 8 0.349633240064108 1104 9 0.3792878859656099 1104 10-11 0.35144133610425854 1104 12-13 0.39173073888480303 1104 14-15 0.3769767388634264 1104 16-17 0.39365033794442184 1104 18-19 0.44856911424167834 1104 20-21 0.489352828843856 1104 22-23 0.4664598423663264 1104 24-25 0.572991814737307 1104 26-27 0.4525163944447428 1104 28-29 0.48453336913353695 1104 30-31 0.4218823511462162 1104 32-33 0.4816551288110844 1104 34-35 0.43210791452284525 1104 36-37 0.38757089503673114 1104 38-39 0.9525563653547096 1104 40-41 0.7985339250789494 1104 42-43 0.6409924492037575 1104 44-45 0.5860624138116108 1104 46-47 0.6483464049906544 1104 48-49 0.6614746402823499 1104 50-51 0.4003712028133748 1104 52-53 0.5965277758136835 1104 54-55 0.5535123068296812 1104 56-57 0.5640438412932127 1104 58-59 0.507785156415892 1104 60-61 0.6346816268913784 1104 62-63 0.5383624245286924 1104 64-65 0.5177607608300363 1104 66-67 0.5903664086473341 1104 68-69 0.4751841566758941 1104 70-71 0.5663950919895342 1104 72-73 0.5241157877528124 1104 74-75 0.44853287119293483 1104 76-77 0.5432324055552975 1104 78-79 0.4785703552027911 1104 80-81 0.33557064706565853 1104 82-83 0.43274058466931464 1104 84-85 0.4512409102261934 1104 86-87 0.37128599126225836 1104 88-89 0.4513679282617922 1104 90-91 0.26336330201859326 1104 92-93 0.34287284297187526 1104 94-95 0.32854309186020814 1104 96-97 0.31079713705957346 1104 98-99 0.1141760211751901 1104 100-101 -0.1747541289568666 1105 1 0.17634818588493673 1105 2 0.20860573910414715 1105 3 0.20532433891044022 1105 4 0.31512305838338506 1105 5 0.1936725284093157 1105 6 0.20793133161419775 1105 7 0.24619873954489435 1105 8 0.2619985850754105 1105 9 0.3060882230224138 1105 10-11 0.3178380010158932 1105 12-13 0.3272055125029496 1105 14-15 0.37035301547744126 1105 16-17 0.32596600005521026 1105 18-19 0.34103099350392796 1105 20-21 0.4274720882741079 1105 22-23 0.4118357164226154 1105 24-25 0.358021557384653 1105 26-27 0.3868839637580237 1105 28-29 0.41540442898614316 1105 30-31 0.35358695565600584 1105 32-33 0.3509968900807081 1105 34-35 0.26735036457555594 1105 36-37 0.39469610981947056 1105 38-39 0.7080148888321247 1105 40-41 0.4860649839990643 1105 42-43 0.29137175182368225 1105 44-45 0.228666552878245 1105 46-47 0.2740838873219005 1105 48-49 0.15169356800049627 1105 50-51 0.04059246172735698 1105 52-53 -0.0012186401148639447 1105 54-55 0.17497102135143905 1105 56-57 0.10732806410095463 1105 58-59 0.18568348671109192 1105 60-61 0.29396888366521523 1105 62-63 0.1959048665075116 1105 64-65 0.1966199418183976 1105 66-67 0.21397727179248704 1105 68-69 0.24108473174210587 1105 70-71 0.21370420969445547 1105 72-73 0.2004769502576309 1105 74-75 0.33024213809439473 1105 76-77 0.22256248810237267 1105 78-79 0.24422592727256287 1105 80-81 0.30734901970901873 1105 82-83 0.17536979830578758 1105 84-85 0.22080743288939786 1105 86-87 0.1627489815383285 1105 88-89 0.15126194413894467 1105 90-91 0.28006762792204754 1105 92-93 0.3070286601015013 1105 94-95 0.31098765204315626 1105 96-97 0.42571849615811885 1105 98-99 0.3642303209806208 1105 100-101 0.09170189616091662 1106 1 0.18644123389310963 1106 2 0.15528125213984012 1106 3 0.17998480469920608 1106 4 0.1399764923104172 1106 5 0.086574993924593 1106 6 0.13341801981757584 1106 7 0.11249285416909771 1106 8 0.16868318255109926 1106 9 0.19413120993053923 1106 10-11 0.34109782558081037 1106 12-13 0.2525249703630834 1106 14-15 0.36904772603394775 1106 16-17 0.29812073254327487 1106 18-19 0.22959407113452102 1106 20-21 0.2466346823302885 1106 22-23 0.23922776357460407 1106 24-25 0.30007199736594714 1106 26-27 0.32196059357885076 1106 28-29 0.24850549795385035 1106 30-31 0.12308333538036464 1106 32-33 0.038507069792359516 1106 34-35 -0.06425695419073918 1106 36-37 -0.1330399571493146 1106 38-39 0.34393167692950044 1106 40-41 0.2142287948301984 1106 42-43 0.20097112192271283 1106 44-45 0.075456231107907 1106 46-47 0.026361312009449023 1106 48-49 -0.055325947432166345 1106 50-51 -0.05442605554919311 1106 52-53 0.0846214789670725 1106 54-55 0.09412190057368264 1106 56-57 0.1873613925857711 1106 58-59 0.24866084441941183 1106 60-61 0.26071051365588005 1106 62-63 0.17860828436474918 1106 64-65 0.17566007884276047 1106 66-67 0.06553900287041614 1106 68-69 0.027828264848928086 1106 70-71 0.13570682353955732 1106 72-73 0.09674329332197118 1106 74-75 0.16005390814918385 1106 76-77 0.28012804980035 1106 78-79 0.42159842066827125 1106 80-81 0.43049642106271335 1106 82-83 0.369301361900785 1106 84-85 0.3314009089898313 1106 86-87 0.40398931244537195 1106 88-89 0.38734546175953355 1106 90-91 0.2543689186441611 1106 92-93 0.2181818788065648 1106 94-95 0.46385033938251397 1106 96-97 0.4611613125261371 1106 98-99 0.5698140071380422 1106 100-101 0.22175810994400535 1107 1 0.12274095845614852 1107 2 0.02710814666488659 1107 3 0.1402902427873265 1107 4 0.08508313453930327 1107 5 0.06456788785576606 1107 6 0.11774985480113997 1107 7 0.061888019175292186 1107 8 0.0605245144782387 1107 9 0.12580553900091473 1107 10-11 0.17334954834173288 1107 12-13 0.21734521958745745 1107 14-15 0.21522493746508786 1107 16-17 0.2764638614662047 1107 18-19 0.23166471201182048 1107 20-21 0.15782254262538942 1107 22-23 0.09170080863773222 1107 24-25 0.2087992021415488 1107 26-27 0.22226065603852874 1107 28-29 0.25351455972749903 1107 30-31 0.21323697596073288 1107 32-33 0.26134043555011743 1107 34-35 0.4226729988109099 1107 36-37 0.3613059056646506 1107 38-39 0.6704615295860847 1107 40-41 0.6036545753202418 1107 42-43 0.2850561899628161 1107 44-45 0.36623272783645433 1107 46-47 0.440118927483077 1107 48-49 0.50219332803551 1107 50-51 0.46929116591645936 1107 52-53 0.6204011498762227 1107 54-55 0.606198429044035 1107 56-57 0.4364251628979616 1107 58-59 0.37206412918436627 1107 60-61 0.34216234290544634 1107 62-63 0.40663588949667684 1107 64-65 0.30720503445542846 1107 66-67 0.262795589295159 1107 68-69 0.314202377870231 1107 70-71 0.35237132288936124 1107 72-73 0.4003579170791838 1107 74-75 0.5038491018253985 1107 76-77 0.4762634337226146 1107 78-79 0.5017079298989948 1107 80-81 0.42022554058597095 1107 82-83 0.2866517914390947 1107 84-85 0.29812699768258355 1107 86-87 0.4975886796571096 1107 88-89 0.36316905923247944 1107 90-91 0.5094681373861611 1107 92-93 0.4583783708250344 1107 94-95 0.42713940484231117 1107 96-97 0.5146779974528748 1107 98-99 0.6065731175483293 1107 100-101 0.3321148468863484 1108 1 -0.001076407848596972 1108 2 0.0338905689373874 1108 3 0.07082423344125388 1108 4 -0.03566346441938606 1108 5 0.04698610973110107 1108 6 0.030289496568002505 1108 7 0.05932288812282138 1108 8 0.0188307613291272 1108 9 0.20142938432639568 1108 10-11 0.16229826350389942 1108 12-13 0.17350098085756827 1108 14-15 0.16221818972010027 1108 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-1.2782022342453736 2316 76-77 -1.2354369039667148 2316 78-79 -1.1809345549434767 2316 80-81 -1.1519338682247415 2316 82-83 -1.0881648495345502 2316 84-85 -0.9714912128273134 2316 86-87 -0.9012541418618234 2316 88-89 -1.047340796960892 2316 90-91 -1.1135803039152492 2316 92-93 -1.1753834315892284 2316 94-95 -1.068914674068008 2316 96-97 -1.0727409986354388 2316 98-99 -1.4504375114613488 2316 100-101 -0.876497871867258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13917.0 3 3420.0 4 3551.0 5 3974.0 6 4445.0 7 5134.0 8 6399.0 9 7807.0 10 8939.0 11 10774.0 12 13041.0 13 16620.0 14 16761.0 15 17344.0 16 20428.0 17 25128.0 18 23324.0 19 18062.0 20 18283.0 21 19642.0 22 21068.0 23 23433.0 24 26493.0 25 30348.0 26 35259.0 27 40968.0 28 48602.0 29 57097.0 30 69073.0 31 84436.0 32 105511.0 33 136303.0 34 187044.0 35 268586.0 36 422621.0 37 660119.0 38 592094.0 39 83446.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24031707324063 18.612019627130376 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27.08707325184842 28.528925110059323 21.934506833121258 20-21 23.18339100346021 26.306403292285772 28.377232597191128 22.132973107062895 22-23 22.88100486974703 26.070551618952752 28.33943741326315 22.709006098037072 24-25 22.686768861554633 26.385323739267836 28.448075391820062 22.479832007357473 26-27 22.55125831736559 26.548794018764355 28.419313701745125 22.480633962124926 28-29 22.395853791147342 26.676310960575172 28.629542867147755 22.29829238112973 30-31 22.6217782196814 26.396190272053232 28.344272886122695 22.637758622142666 32-33 22.431260471702075 26.521283078492765 28.319314875573596 22.728141574231568 34-35 22.954246266793675 26.52857495400156 28.050088237032533 22.467090542172237 36-37 23.02526335165495 26.261648269282638 28.20308364008046 22.510004738981948 38-39 22.668034974771555 26.59150326588952 28.22188085387018 22.518580905468745 40-41 22.73769255003399 26.49079994438896 28.264406256496986 22.50710124908006 42-43 22.608540337878622 26.407031549637633 27.978158372530835 23.006269739952906 44-45 22.748226297162986 26.65361796784457 27.791288438600866 22.806867296391577 46-47 22.83039516896625 26.818639109121854 27.588001393272993 22.762964328638905 48-49 22.886831607205234 26.707782171300604 27.589375579208625 22.81601064228554 50-51 22.721800587453203 26.929909457690353 27.56133552458652 22.78695443026992 52-53 22.89931806403751 26.941040365064552 27.301077287511305 22.858564283386638 54-55 22.65962331215532 26.754290768339416 27.43503425645527 23.151051663049994 56-57 22.7195990646798 27.066705011308905 27.386156903980467 22.827539020030823 58-59 22.82589186802537 26.80553363841986 27.498417981296313 22.87015651225845 60-61 22.83731792435034 26.83332717279916 27.711553805680605 22.61780109716989 62-63 22.635761118298355 26.860466470173446 27.759790621311065 22.743981790217138 64-65 22.52574223346792 26.726864527538822 27.67283319122032 23.07456004777294 66-67 22.563855031165836 26.905872729558126 27.388306733849692 23.141965505426345 68-69 22.44119963090636 26.844861979143403 27.56979213595715 23.14414625399309 70-71 22.657317669429304 26.661391837148145 27.56798189116995 23.113308602252598 72-73 22.686571878021695 26.64703219494019 27.523995708574844 23.142400218463273 74-75 22.510304739542125 27.077234501416502 27.196472780174062 23.215987978867307 76-77 22.696269872899244 26.70018532703021 27.27878011085813 23.324764689212422 78-79 22.669275349146478 26.813046282587607 27.35235471065709 23.16532365760883 80-81 22.68107515473371 27.02270767131924 27.277762975555923 23.018454198391122 82-83 22.75423911258107 26.859811474507918 27.29675940913157 23.08919000377944 84-85 22.734078592352542 26.80079500390788 27.187117913087633 23.278008490651942 86-87 22.616500767632218 26.796575947268682 27.332411462350937 23.254511822748164 88-89 22.596121641906734 26.641909922622947 27.37533005979513 23.386638375675194 90-91 22.60055039994283 26.715246193269117 27.424892676477647 23.25931073031041 92-93 22.696540667299455 26.455862998620095 27.44802226990293 23.39957406417752 94-95 22.613700934263374 26.40824658197688 27.5815554210598 23.396497062699943 96-97 22.545580073261196 26.37264243269409 27.624811307677078 23.456966186367637 98-99 22.6032037413405 26.435930676474957 27.2967880117078 23.664077570476742 100-101 22.70787292233932 25.88920025523854 27.791113316246197 23.611813506175945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3404.0 1 1935.0 2 446.0 3 437.0 4 422.0 5 429.0 6 593.5 7 1080.0 8 1761.0 9 2250.0 10 2348.0 11 2381.5 12 2720.5 13 3149.5 14 3409.5 15 3632.0 16 3880.5 17 4076.0 18 4290.0 19 4532.5 20 4926.5 21 5417.5 22 6242.5 23 7901.0 24 9869.5 25 12209.0 26 15602.0 27 19719.0 28 23538.0 29 27870.0 30 33016.5 31 38313.5 32 43865.0 33 49219.0 34 55223.5 35 61034.5 36 67024.5 37 73630.0 38 79297.5 39 83747.0 40 88144.0 41 94384.0 42 100224.0 43 104412.0 44 108103.5 45 113271.0 46 115941.0 47 120426.5 48 132471.0 49 134419.0 50 125870.5 51 120505.5 52 117600.5 53 115525.0 54 113410.0 55 107100.5 56 94938.0 57 84600.0 58 75928.5 59 64362.5 60 53734.0 61 45326.5 62 37342.5 63 28569.5 64 21115.0 65 15740.0 66 12036.0 67 10229.0 68 8102.0 69 5597.5 70 4059.0 71 3131.5 72 2527.0 73 2011.5 74 1520.0 75 1204.0 76 847.0 77 519.0 78 340.0 79 220.0 80 151.5 81 110.0 82 74.0 83 52.0 84 36.5 85 24.5 86 19.5 87 14.5 88 13.0 89 13.5 90 8.5 91 6.5 92 6.5 93 5.5 94 6.5 95 5.0 96 3.5 97 3.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2909024867050705 2 0.1812345987898431 3 0.18183786742631947 4 0.20253950695171852 5 0.2153669032220578 6 0.11230321932720307 7 0.12722618033477598 8 0.14033933543291985 9 0.14386369430917645 10-11 0.13100454705797 12-13 0.12076485572830559 14-15 0.13500517064723422 16-17 0.11128718793945343 18-19 0.13929155306430305 20-21 0.16475583971977853 22-23 0.1682960740864687 24-25 0.19171242247601133 26-27 0.17175693100099093 28-29 0.1694073584168199 30-31 0.15978681121406543 32-33 0.16894696919424582 34-35 0.3520390003648188 36-37 0.47212121000448004 38-39 0.43698874967494933 40-41 0.4267649338357185 42-43 0.45122906459387796 44-45 0.455007431317072 46-47 0.4586429186263637 48-49 0.4713115599923671 50-51 0.46423109125898676 52-53 0.47088292175066015 54-55 0.4527848626563696 56-57 0.41412804345058224 58-59 0.40304695112793776 60-61 0.3572937876983365 62-63 0.46707280404659896 64-65 0.4661043991301501 66-67 0.4758519502563733 68-69 0.48721880140682244 70-71 0.4802812120873444 72-73 0.47264510118878844 74-75 0.4771854914527947 76-77 0.45214984303902606 78-79 0.439004936960015 80-81 0.43557583102636 82-83 0.44845085376800004 84-85 0.45018128222526116 86-87 0.45145132145994815 88-89 0.45946844412891025 90-91 0.47820152284054435 92-93 0.48369444253056587 94-95 0.4815988777933322 96-97 0.4796779434508681 98-99 0.4768203551728222 100-101 0.45729350193950863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3149509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.56311416875097 #Duplication Level Percentage of deduplicated Percentage of total 1 72.96040380451846 37.620656311705574 2 14.347444276511728 14.795978145191338 3 5.1922073539412095 8.031791417372967 4 2.482276992298618 5.119757278094296 5 1.3769167627872498 3.549905812023297 6 0.8267988932365667 2.557939543593242 7 0.5477791184972478 1.9771638058431948 8 0.4029843327824785 1.6623301727584698 9 0.2748809991525342 1.2756348307910215 >10 1.442467472974343 13.215586129362164 >50 0.0930783844634221 3.28402089394497 >100 0.049374166502382695 4.792747066208038 >500 0.002463594424988549 0.8674602136964795 >1k 8.006681874827628E-4 0.8059774025216058 >5k 1.2317972115119427E-4 0.44305097689345213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7505 0.2382911114081592 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6446 0.20466682266981934 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4052 0.12865497447379892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3810 0.12097123710394224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.175098086717644E-5 0.0 9 0.0 0.0 0.0 3.175098086717644E-5 0.0 10-11 0.0 0.0 0.0 3.175098086717644E-5 0.0 12-13 0.0 0.0 0.0 3.175098086717644E-5 0.0 14-15 0.0 0.0 0.0 7.93774521679411E-5 0.0 16-17 0.0 0.0 0.0 1.2700392346870576E-4 0.0 18-19 0.0 0.0 0.0 1.2700392346870576E-4 0.0 20-21 0.0 0.0 0.0 1.7463039476947042E-4 0.0 22-23 0.0 0.0 0.0 2.8575882780458795E-4 0.0 24-25 0.0 0.0 0.0 4.127627512732937E-4 0.0 26-27 0.0 0.0 0.0 6.032686364763523E-4 0.0 28-29 0.0 0.0 0.0 0.0020638137563664684 0.0 30-31 0.0 0.0 0.0 0.005175409881349759 0.0 32-33 0.0 0.0 0.0 0.012668641366003399 0.0 34-35 0.0 0.0 0.0 0.024321251344257153 0.0 36-37 0.0 0.0 0.0 0.04429261830971113 0.0 38-39 0.0 0.0 0.0 0.0747894354326341 0.0 40-41 0.0 0.0 0.0 0.10792158396753271 0.0 42-43 0.0 0.0 0.0 0.14503848060126195 0.0 44-45 0.0 0.0 0.0 0.19247444601682356 0.0 46-47 0.0 0.0 0.0 0.2517694027862756 0.0 48-49 0.0 0.0 0.0 0.3161921429657766 0.0 50-51 0.0 0.0 0.0 0.3880604881586305 0.0 52-53 0.0 0.0 0.0 0.45950019510977747 0.0 54-55 0.0 0.0 0.0 0.5427830179243813 0.0 56-57 0.0 0.0 0.0 0.6455133165201306 0.0 58-59 0.0 0.0 0.0 0.7588643182159505 0.0 60-61 0.0 0.0 0.0 0.8813913533823843 0.0 62-63 0.0 0.0 0.0 1.0095700631431757 0.0 64-65 0.0 0.0 0.0 1.1471788142215185 0.0 66-67 0.0 0.0 0.0 1.308727804873712 0.0 68-69 0.0 0.0 0.0 1.495709331200514 0.0 70-71 0.0 0.0 0.0 1.7045196568735 0.0 72-73 0.0 0.0 0.0 1.922918778768373 0.0 74-75 0.0 0.0 0.0 2.149890665497384 0.0 76-77 0.0 0.0 0.0 2.4045176565617052 0.0 78-79 0.0 1.587549043358822E-5 0.0 2.6894827098446137 0.0 80-81 0.0 3.175098086717644E-5 0.0 3.00854831657887 0.0 82-83 0.0 3.175098086717644E-5 0.0 3.338298128374931 0.0 84-85 0.0 3.175098086717644E-5 0.0 3.679478293283175 0.0 86-87 0.0 3.175098086717644E-5 0.0 4.04517021542088 0.0 88-89 0.0 3.175098086717644E-5 0.0 4.450344482266918 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11000 0.0 25.666723 1 GGTATCA 11075 0.0 25.407217 1 GTATCAA 15010 0.0 25.132318 1 TACATGG 11190 0.0 24.661196 2 ACATGGG 11275 0.0 23.381884 3 GAGTACT 8185 0.0 21.709713 12-13 ATCAACG 17315 0.0 21.441765 3 TCAACGC 17355 0.0 21.392006 4 CAACGCA 17485 0.0 21.232283 5 TATCAAC 17685 0.0 21.019978 2 AACGCAG 17840 0.0 20.877218 6 CATGGGG 6775 0.0 20.855534 4 GTACTTT 8675 0.0 20.241035 14-15 GTATACG 260 1.5103069E-8 20.075459 1 ATTCGCG 345 0.0 19.977245 80-81 ACGCAGA 19690 0.0 18.795343 7 AGAGTAC 14595 0.0 18.699152 10-11 CGCAGAG 19790 0.0 18.652483 8 CGCGGAA 370 0.0 18.627432 82-83 AGTACTT 8880 0.0 18.4631 12-13 >>END_MODULE