FastQCFastQC Report
Fri 27 May 2016
SRR939323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149509
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77540.2461971056440861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.22597173083169472No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45850.14557824727600396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44300.14065684524159164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124700.023.5517561
GTACATG115000.023.385961
GTATCAA164350.023.2857861
TACATGG117600.022.2342782
ACATGGG118550.021.0954043
GAGTACT94200.020.68020412-13
GTACTTT96900.019.88359314-15
ATCAACG191650.019.784123
TCAACGC191800.019.7439064
CAACGCA192250.019.7223765
TATCAAC195550.019.4623532
AACGCAG196900.019.2807126
AGAGTAC163300.018.88946710-11
CATGGGG69750.018.8457894
AGTACTT99200.018.77675612-13
ACGCAGA220000.017.1483867
CGTACAC3351.4772013E-816.998683
CGCAGAG221850.016.9412168
GTGTAGC17300.016.7837241
ATGGGGA44500.016.5291485
Produced by FastQC (version 0.11.5)