##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3149509 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32423307887039 33.0 31.0 34.0 31.0 34.0 2 32.53216485490278 34.0 31.0 34.0 31.0 34.0 3 32.622025210913826 34.0 31.0 34.0 31.0 34.0 4 36.14066986314375 37.0 35.0 37.0 35.0 37.0 5 36.08228838209384 37.0 35.0 37.0 35.0 37.0 6 36.10999333546911 37.0 35.0 37.0 35.0 37.0 7 36.05587378858101 37.0 35.0 37.0 35.0 37.0 8 36.028143751930855 37.0 35.0 37.0 35.0 37.0 9 37.73733239054088 39.0 38.0 39.0 35.0 39.0 10-11 37.6593013387166 39.0 37.0 39.0 35.0 39.0 12-13 37.58372717779184 39.0 37.0 39.0 35.0 39.0 14-15 38.89254991809835 40.0 38.0 41.0 35.0 41.0 16-17 38.8908895005539 40.0 38.0 41.0 35.0 41.0 18-19 38.98297623534335 40.0 38.5 41.0 35.0 41.0 20-21 38.96653780001898 40.0 38.0 41.0 35.0 41.0 22-23 38.893330833472774 40.0 38.0 41.0 35.0 41.0 24-25 38.82516084253133 40.0 38.0 41.0 35.0 41.0 26-27 38.67772341657064 40.0 38.0 41.0 34.0 41.0 28-29 38.51492645361547 40.0 38.0 41.0 34.0 41.0 30-31 38.332252106598204 40.0 38.0 41.0 34.0 41.0 32-33 38.299113766622035 40.0 38.0 41.0 34.0 41.0 34-35 38.21280809167397 40.0 38.0 41.0 34.0 41.0 36-37 38.07047098452489 40.0 38.0 41.0 33.0 41.0 38-39 37.94788187619086 40.0 37.5 41.0 33.0 41.0 40-41 37.77842355744975 40.0 37.0 41.0 33.0 41.0 42-43 37.60460963915328 40.0 37.0 41.0 33.0 41.0 44-45 37.374746031841795 40.0 37.0 41.0 32.0 41.0 46-47 37.08985718091296 40.0 36.5 41.0 31.0 41.0 48-49 36.792529565719605 40.0 36.0 41.0 31.0 41.0 50-51 36.547345951384806 39.0 36.0 41.0 30.0 41.0 52-53 36.28920666681695 39.0 35.0 41.0 30.0 41.0 54-55 36.03966332529927 39.0 35.0 40.0 29.5 41.0 56-57 35.72024702898134 38.5 35.0 40.0 28.5 41.0 58-59 35.40374436142268 38.0 34.5 40.0 28.0 41.0 60-61 35.05904682285397 38.0 34.0 40.0 28.0 41.0 62-63 34.70622658960492 37.0 34.0 40.0 27.0 41.0 64-65 34.389881406911364 37.0 34.0 39.5 26.0 41.0 66-67 33.970792907719904 36.0 33.0 39.0 26.0 40.5 68-69 33.49841324473117 36.0 33.0 39.0 25.5 40.0 70-71 33.01255798919768 35.0 32.0 38.0 24.5 40.0 72-73 32.518183469232824 35.0 32.0 37.5 23.5 39.5 74-75 31.992584240908663 35.0 31.0 37.0 22.0 39.0 76-77 30.04595367087378 33.0 29.0 35.0 20.0 37.5 78-79 31.01337922831781 34.0 30.5 36.0 20.0 38.0 80-81 31.025629709265793 34.0 31.0 35.5 20.0 37.0 82-83 30.669374178641814 34.0 31.0 35.0 19.0 37.0 84-85 30.213681561157628 34.0 30.0 35.0 17.0 36.5 86-87 29.604366109130027 34.0 29.5 35.0 9.0 36.0 88-89 29.013351287454647 34.0 29.0 35.0 4.0 35.5 90-91 28.523877690141543 33.5 29.0 35.0 2.0 35.0 92-93 28.151770641074528 33.0 28.5 35.0 2.0 35.0 94-95 27.613433554246075 33.0 27.0 35.0 2.0 35.0 96-97 26.85500993964456 33.0 25.5 34.5 2.0 35.0 98-99 25.82200844639593 32.0 24.5 34.0 2.0 35.0 100-101 21.45185328887773 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2555019168445867 1101 2 0.264233525533065 1101 3 0.24059901391085248 1101 4 0.19540472354957927 1101 5 0.15972469619313046 1101 6 0.20303706057586624 1101 7 0.25416261079849534 1101 8 0.2277367128466139 1101 9 0.28404805898364316 1101 10-11 0.3244384509835001 1101 12-13 0.2847599764249438 1101 14-15 0.4300703552333971 1101 16-17 0.3138290551504639 1101 18-19 0.306552388309413 1101 20-21 0.35948377100748274 1101 22-23 0.34719659606380304 1101 24-25 0.3417219125998088 1101 26-27 0.28278437500371467 1101 28-29 0.3314059334846675 1101 30-31 0.34511767351563805 1101 32-33 0.42822426389301427 1101 34-35 0.47087282843154554 1101 36-37 0.4538969955413421 1101 38-39 0.4970449129289207 1101 40-41 0.6087482938739512 1101 42-43 0.6323492171363654 1101 44-45 0.6155153731544587 1101 46-47 0.6612165948137161 1101 48-49 0.6067045162874223 1101 50-51 0.5948772019581838 1101 52-53 0.5825420952107407 1101 54-55 0.559781309295019 1101 56-57 0.45710087049955916 1101 58-59 0.48529176501393323 1101 60-61 0.35176016839159274 1101 62-63 0.49875384778734144 1101 64-65 0.3769822985650819 1101 66-67 0.35555169814315235 1101 68-69 0.48075022830285974 1101 70-71 0.3877674235180777 1101 72-73 0.41127443832970556 1101 74-75 0.3259714168113348 1101 76-77 0.4217933284324218 1101 78-79 0.44010721694293764 1101 80-81 0.6951148034453567 1101 82-83 0.7688194323929736 1101 84-85 0.7377105397066082 1101 86-87 0.5678566184368208 1101 88-89 0.6695191585380798 1101 90-91 0.921727132840207 1101 92-93 0.7955091146401934 1101 94-95 0.563378654531963 1101 96-97 0.48928587631047904 1101 98-99 0.484521074681723 1101 100-101 0.3509311813998828 1102 1 0.19803115051624331 1102 2 0.22884757233781983 1102 3 0.20381630902861758 1102 4 0.1364976113965639 1102 5 0.19017532728384623 1102 6 0.20279451535513005 1102 7 0.23441011649232735 1102 8 0.21509447900015033 1102 9 0.331988093039584 1102 10-11 0.27880284554431256 1102 12-13 0.29190933298286126 1102 14-15 0.3523978629990765 1102 16-17 0.3872749354426972 1102 18-19 0.35750356898667235 1102 20-21 0.31703798384862836 1102 22-23 0.34426152497115936 1102 24-25 0.3167822127283557 1102 26-27 0.4190697625755391 1102 28-29 0.38269478492099296 1102 30-31 0.2632717350524061 1102 32-33 0.34878734233472386 1102 34-35 0.356894628615116 1102 36-37 0.42695281132252205 1102 38-39 0.3546907899340894 1102 40-41 0.38701029052413816 1102 42-43 0.5120033337064882 1102 44-45 0.582947949816436 1102 46-47 0.5573937932719844 1102 48-49 0.5292419476887105 1102 50-51 0.5663341865383913 1102 52-53 0.47923065569240464 1102 54-55 0.48850152715739625 1102 56-57 0.4667718008821069 1102 58-59 0.5753920851038998 1102 60-61 0.4948766653015113 1102 62-63 0.3961976851216775 1102 64-65 0.409092397161956 1102 66-67 0.43438761057386444 1102 68-69 0.42379418576430794 1102 70-71 0.24317822390236188 1102 72-73 0.1730954050940383 1102 74-75 0.22601987605153795 1102 76-77 0.2827872757587713 1102 78-79 0.20931871587738016 1102 80-81 0.372255115606535 1102 82-83 0.34719938969107034 1102 84-85 0.33878017907125013 1102 86-87 0.21883566442229707 1102 88-89 0.38392527386509556 1102 90-91 0.5614973025670729 1102 92-93 0.3983350901874516 1102 94-95 0.3384682642831791 1102 96-97 0.21399954096954943 1102 98-99 -0.012012944153134697 1102 100-101 -0.19826714937103773 1103 1 0.09029772067418662 1103 2 0.145862212878761 1103 3 0.18418535578952344 1103 4 0.10118597320319367 1103 5 0.14544393289388324 1103 6 0.13408977084684892 1103 7 0.15515815318644854 1103 8 0.15423094396211212 1103 9 0.16278497612506726 1103 10-11 0.1814452175779877 1103 12-13 0.27187361782129926 1103 14-15 0.36368815681313293 1103 16-17 0.2706594089834269 1103 18-19 0.23648718554188264 1103 20-21 0.2528329335395014 1103 22-23 0.2236604931502839 1103 24-25 0.20422396627391493 1103 26-27 0.2067728773845019 1103 28-29 0.21675038543633462 1103 30-31 0.26706266239069976 1103 32-33 0.3296031382554503 1103 34-35 0.38592874132571353 1103 36-37 0.24728087655805098 1103 38-39 0.34050814520383454 1103 40-41 0.3971840210790347 1103 42-43 0.4294694918693125 1103 44-45 0.42705354858539835 1103 46-47 0.4174960959808871 1103 48-49 0.299512494124734 1103 50-51 0.3594343953350929 1103 52-53 0.3049316629649894 1103 54-55 0.4385232143705622 1103 56-57 0.5034355148179799 1103 58-59 0.45319182257838264 1103 60-61 0.5353397907224675 1103 62-63 0.5006976641899854 1103 64-65 0.5957618951803809 1103 66-67 0.3604770059848974 1103 68-69 0.24184942858307323 1103 70-71 0.29975193900001074 1103 72-73 0.43457049229495937 1103 74-75 0.4090337595836502 1103 76-77 0.37851074558406594 1103 78-79 0.39484830375097246 1103 80-81 0.5595381970288287 1103 82-83 0.6151502341547364 1103 84-85 0.5278238138432236 1103 86-87 0.5498416167015101 1103 88-89 0.6416233056173546 1103 90-91 0.6907077616746058 1103 92-93 0.6036602980614312 1103 94-95 0.36310195350479546 1103 96-97 0.07795816706787306 1103 98-99 -0.020541555297064917 1103 100-101 -0.02994217261264609 1104 1 0.06850012824787655 1104 2 0.11120025906799924 1104 3 0.08832049463475045 1104 4 0.04138444322855861 1104 5 0.09042272682508923 1104 6 0.06374588013787985 1104 7 0.1290886833292575 1104 8 0.12569673287125482 1104 9 0.12273037577195822 1104 10-11 0.05492488477018043 1104 12-13 0.12909315961332624 1104 14-15 0.15523490732552858 1104 16-17 0.11929425159143392 1104 18-19 0.06067446451533698 1104 20-21 0.14973281248930448 1104 22-23 0.16279155836228654 1104 24-25 0.08775115621575935 1104 26-27 0.22244528239694006 1104 28-29 0.10415516314550644 1104 30-31 0.14015273455135713 1104 32-33 0.18465126417617483 1104 34-35 0.12235938289175152 1104 36-37 0.21043051111958277 1104 38-39 0.18597007025254442 1104 40-41 0.25723204894732277 1104 42-43 0.24496676927987693 1104 44-45 0.25643558644789977 1104 46-47 0.2433586454750909 1104 48-49 0.20224037245413484 1104 50-51 0.19597533104062848 1104 52-53 0.2918414838380343 1104 54-55 0.3157124728148091 1104 56-57 0.20550929422851283 1104 58-59 0.17471831486225398 1104 60-61 0.30567435159311884 1104 62-63 0.3823986422779413 1104 64-65 0.2487874072369678 1104 66-67 0.28805594859925776 1104 68-69 0.2251401041524943 1104 70-71 0.23789974622059162 1104 72-73 0.21444032619412923 1104 74-75 0.18110075851933516 1104 76-77 0.19052430891078842 1104 78-79 0.3879309714666981 1104 80-81 0.4479554701845103 1104 82-83 0.4366318005929877 1104 84-85 0.2850545870966563 1104 86-87 0.2616134361923166 1104 88-89 0.46868292684968793 1104 90-91 0.625896881062662 1104 92-93 0.5790961411608855 1104 94-95 0.6447340571812852 1104 96-97 0.5412764518276134 1104 98-99 0.5411982634425954 1104 100-101 0.48184272053693533 1105 1 0.13383797538283915 1105 2 0.08171722733075626 1105 3 0.07098458523525153 1105 4 0.06822571835856905 1105 5 0.06947642051960656 1105 6 0.08085651641788161 1105 7 0.07311269123485431 1105 8 0.0675031480947581 1105 9 0.12754878698808625 1105 10-11 0.13026430119794696 1105 12-13 0.15822389648727864 1105 14-15 0.17907309049383713 1105 16-17 0.1376661216767303 1105 18-19 0.15236875490501234 1105 20-21 0.1821783961240797 1105 22-23 0.20759532284555604 1105 24-25 0.2900863889998817 1105 26-27 0.23429725259858003 1105 28-29 0.16329453112988546 1105 30-31 0.23784024514123558 1105 32-33 0.15960358837842392 1105 34-35 0.28112786330458306 1105 36-37 0.23966336574279978 1105 38-39 0.2934731661943317 1105 40-41 0.32699490378659846 1105 42-43 0.32135698644498234 1105 44-45 0.20102101454074983 1105 46-47 0.20563358557472355 1105 48-49 0.32009466020557653 1105 50-51 0.28274158715148445 1105 52-53 0.2478546044904988 1105 54-55 0.19455357282083696 1105 56-57 0.31092858538577417 1105 58-59 0.3039081276501676 1105 60-61 0.1595213933056101 1105 62-63 0.25090168477279917 1105 64-65 0.2446352631622446 1105 66-67 0.21736931476643662 1105 68-69 0.24736777214927486 1105 70-71 0.18447931334595324 1105 72-73 0.1980612873973442 1105 74-75 0.11671739393281655 1105 76-77 0.28967006778326265 1105 78-79 0.29204115401639186 1105 80-81 0.4084816153986779 1105 82-83 0.36894808814656344 1105 84-85 0.5359003563173665 1105 86-87 0.4317209668645994 1105 88-89 0.517575040243603 1105 90-91 0.7126131017507156 1105 92-93 0.7049947602969375 1105 94-95 0.8496716436771798 1105 96-97 0.672183616786441 1105 98-99 0.6941145894324734 1105 100-101 0.2835535339878241 1106 1 0.020710349888226176 1106 2 0.0853925931689119 1106 3 0.0687811948142425 1106 4 -0.012285240019366483 1106 5 -0.041927513051625453 1106 6 0.09811978314070302 1106 7 0.07544041567408755 1106 8 0.021139406420346063 1106 9 0.11077153345033253 1106 10-11 0.08630645543831861 1106 12-13 0.1114066949302881 1106 14-15 0.08826233253102345 1106 16-17 0.057640305492490995 1106 18-19 0.01658163564800219 1106 20-21 0.030276194519196054 1106 22-23 0.11036133014271599 1106 24-25 0.10450687053617003 1106 26-27 0.056428185247426654 1106 28-29 0.09444037689299734 1106 30-31 0.07533568332393514 1106 32-33 0.02651273178454261 1106 34-35 0.14278765059106746 1106 36-37 0.10294929391910301 1106 38-39 0.21755255008987717 1106 40-41 0.1945901706452915 1106 42-43 0.10849126805565845 1106 44-45 0.2054722159426987 1106 46-47 0.23770305236252653 1106 48-49 0.1669442190920023 1106 50-51 0.2062239064621494 1106 52-53 0.21772581535141455 1106 54-55 0.2492558592602876 1106 56-57 0.3016385914217494 1106 58-59 0.40389750155544135 1106 60-61 0.33612197059868976 1106 62-63 0.3520484973363267 1106 64-65 0.2708807372733304 1106 66-67 0.3150175727401532 1106 68-69 0.3196261961600655 1106 70-71 0.26462706832214167 1106 72-73 0.2830840899514371 1106 74-75 0.2851788834848321 1106 76-77 0.32980045083655796 1106 78-79 0.3160976090640908 1106 80-81 0.33768928761326933 1106 82-83 0.32336649672339135 1106 84-85 0.32419185883845003 1106 86-87 0.37480908419061976 1106 88-89 0.6568742144566855 1106 90-91 0.5706060153649197 1106 92-93 0.5916897791843816 1106 94-95 0.639200389440898 1106 96-97 0.5198948038867108 1106 98-99 0.639125311336251 1106 100-101 0.35836918866413114 1107 1 0.031079169195436407 1107 2 0.06500585510024592 1107 3 0.021241901530196117 1107 4 -0.007764387700682107 1107 5 0.04622449948126928 1107 6 0.01512857869275308 1107 7 0.017061090853019323 1107 8 0.0072854392065622164 1107 9 0.050360016294398235 1107 10-11 0.0618548207353129 1107 12-13 0.05952455942350099 1107 14-15 0.13860028037206007 1107 16-17 0.10924572412595523 1107 18-19 0.13086309331364987 1107 20-21 0.13130360510456995 1107 22-23 0.15411730732957807 1107 24-25 0.11515112680517348 1107 26-27 0.06449787387055039 1107 28-29 0.07765034382008196 1107 30-31 0.09435537348294787 1107 32-33 0.08192998294413911 1107 34-35 0.09395544187334082 1107 36-37 0.09442928122238214 1107 38-39 0.06809779240273173 1107 40-41 0.12372510403869086 1107 42-43 0.1409704054976757 1107 44-45 0.09672274070642572 1107 46-47 0.0873047438936041 1107 48-49 0.054396836767068635 1107 50-51 0.20950580441736832 1107 52-53 0.18847824974314875 1107 54-55 0.1690905458867462 1107 56-57 0.2658231853826081 1107 58-59 0.18770068187961897 1107 60-61 0.22685024026068845 1107 62-63 0.05056088176031892 1107 64-65 0.09203219688443909 1107 66-67 0.19523346821745946 1107 68-69 0.316063997579235 1107 70-71 0.34448638044649016 1107 72-73 0.2619394906661441 1107 74-75 0.18384931138486138 1107 76-77 0.13029250002611903 1107 78-79 0.29249629638682606 1107 80-81 0.2610044120718662 1107 82-83 0.3018492383962865 1107 84-85 0.23057682550955505 1107 86-87 0.44174364487378526 1107 88-89 0.8384937633134655 1107 90-91 0.7326260229419965 1107 92-93 0.7386940182513797 1107 94-95 0.8423298755308188 1107 96-97 0.8348844622015292 1107 98-99 0.750914986033937 1107 100-101 0.6854682435774571 1108 1 -0.01750064289517894 1108 2 -0.0479088150703717 1108 3 -0.04010241129645209 1108 4 -0.037203129438893257 1108 5 -0.03245114441958208 1108 6 0.017990788356947007 1108 7 0.02047201172755564 1108 8 8.218305955267624E-4 1108 9 0.08349964422986744 1108 10-11 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-0.528884355048092 2316 82-83 -0.4205190435206241 2316 84-85 -0.4567489869604415 2316 86-87 -0.5359073581668348 2316 88-89 -0.9747631864625887 2316 90-91 -1.1062438623408646 2316 92-93 -0.8252714713981497 2316 94-95 -0.5065400300093508 2316 96-97 -0.4226103975064852 2316 98-99 -0.33959025149313504 2316 100-101 0.17037796688288154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 21.0 5 52.0 6 120.0 7 308.0 8 760.0 9 1493.0 10 2517.0 11 3890.0 12 5572.0 13 7922.0 14 9954.0 15 13061.0 16 16887.0 17 23458.0 18 23499.0 19 17080.0 20 16095.0 21 17441.0 22 18637.0 23 20928.0 24 24242.0 25 28688.0 26 34378.0 27 41913.0 28 51079.0 29 62247.0 30 76012.0 31 93471.0 32 115742.0 33 149532.0 34 204939.0 35 294703.0 36 468295.0 37 734596.0 38 537093.0 39 32870.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.24166343713787 19.310108961170183 15.207275244698504 23.24095235699344 2 15.496129714187196 20.402767542496306 39.72644624924075 24.374656494075744 3 19.20321548533438 23.98977110400383 30.103993987634265 26.703019423027524 4 13.302644951959177 17.00515858186149 36.54934785072848 33.14284861545085 5 14.182020118056496 37.01034034193901 34.018350161882374 14.789289378122113 6 32.32684840716441 35.903088386157975 17.234273659799033 14.535789546878577 7 28.72828748862124 32.345009968220445 20.515229516727846 18.41147302643047 8 26.175953140632398 35.65673252560955 19.432267061310192 18.735047272447865 9 26.005958389069534 17.12120841693102 20.236900418446176 36.63593277555327 10-11 25.233742783398938 26.54658234029495 26.928086250904503 21.291588625401612 12-13 25.82192970396338 23.75741742601783 27.631910243787207 22.78874262623158 14-15 22.70837917448149 25.42982724356501 25.809193503781437 26.052600078172055 16-17 22.49552982258837 28.411261719580374 26.53054064261648 22.56266781521478 18-19 22.329422455458094 27.150845243666783 28.590404336426094 21.929327964449026 20-21 23.073981225959542 26.302865986867474 28.545342868577748 22.077809918595236 22-23 22.74905390014761 26.1099269759191 28.521334595328984 22.6196845286043 24-25 22.62495519142825 26.471396017601474 28.603077495571533 22.300571295398743 26-27 22.47996273702519 26.583065222588804 28.55159553542824 22.38537650495776 28-29 22.29931713165449 26.744898966791332 28.713221648199767 22.24256225335441 30-31 22.54770505497841 26.456219048746966 28.465103608213216 22.53097228806141 32-33 22.392029995786643 26.55332942372922 28.44733893441803 22.607301646066098 34-35 22.875806824692674 26.54722927942738 28.22326236509396 22.353701530785987 36-37 22.92459481974498 26.31889882230559 28.31755151589436 22.438954842055075 38-39 22.57217047721198 26.652130647630766 28.365526583006766 22.410172292150488 40-41 22.687525649706572 26.491390033506356 28.39487807607951 22.426206240707565 42-43 22.570455627877443 26.500904905540562 28.06329576123194 22.865343705350057 44-45 22.71758712724728 26.623890211508332 27.934029618914142 22.724493042330245 46-47 22.804093662411763 26.859765947161495 27.691643362721575 22.644497027705167 48-49 22.921754122486306 26.716362464458587 27.647845130727305 22.714038282327802 50-51 22.72783053472028 26.961731941178723 27.599562026622266 22.71087549747873 52-53 22.884664629204607 27.00478630541007 27.3144697431169 22.796079322268426 54-55 22.679217617730256 26.832166537704765 27.36412247115344 23.12449337341154 56-57 22.69278799091787 27.116498168112035 27.350107938350515 22.840605902619572 58-59 22.843170345034956 26.814769821641814 27.4598191620185 22.882240671304725 60-61 22.817158145788387 26.906233534037817 27.6623243227196 22.61428399745419 62-63 22.637060650632765 26.872690585486936 27.701035649620692 22.78921311425961 64-65 22.48869942722809 26.71645432930249 27.63329579837616 23.16155044509326 66-67 22.549710350389507 26.90293205736158 27.424984005372288 23.122373586876627 68-69 22.448112905758244 26.844963983491134 27.537702116093925 23.1692209946567 70-71 22.602126236184752 26.752487451218588 27.476822577741483 23.16856373485518 72-73 22.65672204778586 26.67501505790268 27.458200627462887 23.210062266848578 74-75 22.477919967512392 27.066032684147384 27.090020554000727 23.366026794339497 76-77 22.58088545201499 26.71254069898477 27.26897238008045 23.43760146891979 78-79 22.52325722982774 26.859177372186643 27.28006897592939 23.33749642205623 80-81 22.54954147592917 27.025511131502306 27.249466781711106 23.175480610857413 82-83 22.59650631257126 26.849550199729542 27.26458314613484 23.289360341564354 84-85 22.551499297192038 26.836849807382674 27.13184817061961 23.479802724805676 86-87 22.40508753235133 26.756141885765732 27.26754918157192 23.571221400311018 88-89 22.298563736293506 26.653233067370373 27.310188660002453 23.73801453633366 90-91 22.31295902372954 26.69491134177649 27.354638306733158 23.637491327760813 92-93 22.284060785347812 26.55952086499832 27.350152039571885 23.806266310081984 94-95 22.176774220997622 26.407005028402843 27.494777757421872 23.921442993177667 96-97 22.078346180309374 26.38593825259747 27.554914115184303 23.980801451908853 98-99 22.055467728758817 26.486827938467826 27.183825913071473 24.273878419701877 100-101 22.064696311271046 25.810354946873304 27.853377973230348 24.2715707686253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 180.0 2 148.0 3 212.0 4 197.5 5 188.0 6 380.5 7 845.0 8 1438.5 9 1788.0 10 1848.0 11 1899.5 12 2071.0 13 2372.0 14 2718.0 15 2950.0 16 3172.0 17 3463.5 18 3793.0 19 4101.5 20 4496.0 21 5219.5 22 6314.0 23 7931.0 24 9838.0 25 12270.5 26 15767.5 27 19855.5 28 23803.0 29 28074.5 30 33141.0 31 38592.0 32 44271.0 33 49762.0 34 55740.5 35 61384.0 36 67315.0 37 74235.5 38 80093.5 39 83973.0 40 88228.5 41 94936.5 42 101240.0 43 105490.0 44 108827.0 45 114309.0 46 117285.5 47 121760.0 48 133444.5 49 135273.5 50 126736.5 51 121359.0 52 118512.0 53 116381.5 54 114226.5 55 107196.0 56 94792.5 57 84717.5 58 76135.5 59 64419.0 60 53332.0 61 44510.0 62 36548.5 63 27933.5 64 20510.5 65 15417.0 66 11846.0 67 9934.0 68 7717.0 69 5194.5 70 3715.0 71 2905.5 72 2332.0 73 1867.5 74 1482.0 75 1216.5 76 812.5 77 424.0 78 233.0 79 138.5 80 86.5 81 45.0 82 22.0 83 15.5 84 15.0 85 10.5 86 4.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29261703967189806 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014605451198901162 16-17 8.096500121129992E-4 18-19 9.525294260152932E-5 20-21 1.587549043358822E-5 22-23 0.0 24-25 0.0 26-27 6.350196173435288E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015399225720580572 36-37 0.008334632477633816 38-39 0.002889339258913056 40-41 0.003381479462354291 42-43 2.8575882780458795E-4 44-45 0.0011112843303511754 46-47 2.0638137563664684E-4 48-49 4.4451373214047016E-4 50-51 7.937745216794109E-5 52-53 7.937745216794109E-5 54-55 0.0 56-57 8.255255025465874E-4 58-59 0.0019050588520305861 60-61 6.191441269099405E-4 62-63 0.00115891080165194 64-65 1.587549043358822E-5 66-67 4.4451373214047016E-4 68-69 0.0016034245337924102 70-71 0.0 72-73 0.0 74-75 1.587549043358822E-5 76-77 0.2588657470100895 78-79 1.4287941390229397E-4 80-81 4.762647130076466E-5 82-83 0.0 84-85 0.0 86-87 0.10752469670669301 88-89 0.3763761271995095 90-91 0.2775035727791221 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.350196173435288E-5 100-101 3.0163431823817616E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3149509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.283753636647695 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46897463163414 34.72975584772633 2 15.579875523409696 15.35669493970722 3 5.701655434628064 8.429969452837888 4 2.755028640373975 5.431126110963977 5 1.5036076951883552 3.7051715607915274 6 0.9106572103498898 2.692836336139249 7 0.6035185746825625 2.082056252485732 8 0.41805415428989984 1.648262235744042 9 0.3412130805134044 1.5134635257861828 >10 1.557123468337968 13.618045176830751 >50 0.10169907608379994 3.4299471541073387 >100 0.054655000470529284 5.028186453709786 >500 0.0027110724856471064 0.8901729008798221 >1k 0.00109733886234867 0.9712307428563093 >5k 1.290986896880788E-4 0.47308130943387405 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7754 0.2461971056440861 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 0.22597173083169472 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4585 0.14557824727600396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4430 0.14065684524159164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.175098086717644E-5 0.0 9 0.0 0.0 0.0 3.175098086717644E-5 0.0 10-11 0.0 1.587549043358822E-5 0.0 3.175098086717644E-5 0.0 12-13 0.0 3.175098086717644E-5 0.0 3.175098086717644E-5 0.0 14-15 0.0 3.175098086717644E-5 0.0 7.93774521679411E-5 0.0 16-17 0.0 3.175098086717644E-5 0.0 1.2700392346870576E-4 0.0 18-19 0.0 3.175098086717644E-5 0.0 1.2700392346870576E-4 0.0 20-21 0.0 3.175098086717644E-5 0.0 1.587549043358822E-4 0.0 22-23 0.0 3.175098086717644E-5 0.0 2.2225686607023508E-4 0.0 24-25 0.0 3.175098086717644E-5 0.0 3.4926078953894085E-4 0.0 26-27 0.0 3.175098086717644E-5 0.0 5.715176556091759E-4 0.0 28-29 0.0 3.175098086717644E-5 0.0 0.002079689246800057 0.0 30-31 0.0 3.175098086717644E-5 0.0 0.005334164785685642 0.0 32-33 0.0 3.175098086717644E-5 0.0 0.01292264921294081 0.0 34-35 0.0 3.175098086717644E-5 0.0 0.024686387624229682 0.0 36-37 0.0 3.175098086717644E-5 0.0 0.044975264398355426 0.0 38-39 0.0 3.175098086717644E-5 0.0 0.07580546682038375 0.0 40-41 0.0 3.175098086717644E-5 0.0 0.10925512516395414 0.0 42-43 0.0 3.175098086717644E-5 0.0 0.14673715807765592 0.0 44-45 0.0 3.175098086717644E-5 0.0 0.19487164507229537 0.0 46-47 0.0 3.175098086717644E-5 0.0 0.25469049302605584 0.0 48-49 0.0 3.175098086717644E-5 0.0 0.31970062635159957 0.0 50-51 0.0 4.762647130076466E-5 0.0 0.39193410782442595 0.0 52-53 0.0 6.350196173435288E-5 0.0 0.46353256967990886 0.0 54-55 0.0 6.350196173435288E-5 0.0 0.5472757817170867 0.0 56-57 0.0 6.350196173435288E-5 0.0 0.6507204773823475 0.0 58-59 0.0 6.350196173435288E-5 0.0 0.7651827634085187 0.0 60-61 0.0 6.350196173435288E-5 0.0 0.888694078981835 0.0 62-63 0.0 6.350196173435288E-5 0.0 1.0173808044365011 0.0 64-65 0.0 6.350196173435288E-5 0.0 1.1560849643547613 0.0 66-67 0.0 6.350196173435288E-5 0.0 1.3181895971721307 0.0 68-69 0.0 6.350196173435288E-5 0.0 1.505885520568444 0.0 70-71 0.0 6.350196173435288E-5 0.0 1.7150609825214027 0.0 72-73 0.0 6.350196173435288E-5 0.0 1.9331902210789047 0.0 74-75 0.0 6.350196173435288E-5 0.0 2.1610035088008956 0.0 76-77 0.0 6.350196173435288E-5 0.0 2.417503807736381 0.0 78-79 0.0 6.350196173435288E-5 0.0 2.7032943865218355 0.0 80-81 0.0 6.350196173435288E-5 0.0 3.022614001103029 0.0 82-83 0.0 6.350196173435288E-5 0.0 3.35214155603302 0.0 84-85 0.0 6.350196173435288E-5 0.0 3.6946870131185525 0.0 86-87 0.0 6.350196173435288E-5 0.0 4.061458468605742 0.0 88-89 0.0 6.350196173435288E-5 0.0 4.4678075217438655 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12470 0.0 23.551756 1 GTACATG 11500 0.0 23.38596 1 GTATCAA 16435 0.0 23.285786 1 TACATGG 11760 0.0 22.234278 2 ACATGGG 11855 0.0 21.095404 3 GAGTACT 9420 0.0 20.680204 12-13 GTACTTT 9690 0.0 19.883593 14-15 ATCAACG 19165 0.0 19.78412 3 TCAACGC 19180 0.0 19.743906 4 CAACGCA 19225 0.0 19.722376 5 TATCAAC 19555 0.0 19.462353 2 AACGCAG 19690 0.0 19.280712 6 AGAGTAC 16330 0.0 18.889467 10-11 CATGGGG 6975 0.0 18.845789 4 AGTACTT 9920 0.0 18.776756 12-13 ACGCAGA 22000 0.0 17.148386 7 CGTACAC 335 1.4772013E-8 16.99868 3 CGCAGAG 22185 0.0 16.941216 8 GTGTAGC 1730 0.0 16.783724 1 ATGGGGA 4450 0.0 16.529148 5 >>END_MODULE