FastQCFastQC Report
Fri 27 May 2016
SRR939322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207980
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76120.23728327483338424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65150.20308730104302394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42400.13217040006483832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.1283673838365576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113800.027.5880621
GTATCAA157050.025.9182031
TCAACGC180000.022.3672224
ATCAACG179450.022.3564993
CAACGCA180700.022.228095
GTACATG111800.022.1339021
GAGTACT85900.021.90297912-13
TATCAAC185050.021.83372
AACGCAG185700.021.7168276
TACATGG116800.020.8281822
GTACTTT89200.020.77453414-15
ACATGGG116600.020.2945233
ACGCAGA204950.019.584587
AGTACTT90400.019.31856512-13
CGCAGAG207750.019.2293828
AGAGTAC153050.018.90286610-11
GCAGAGT218250.017.8042959
GAGTACA101350.017.011591
CATGGGG70850.016.8000564
AGTACAT91150.016.7523632
Produced by FastQC (version 0.11.5)