##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3207980 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.788344690428243 33.0 31.0 34.0 30.0 34.0 2 31.873254820790653 33.0 31.0 34.0 30.0 34.0 3 31.92634461561481 34.0 31.0 34.0 30.0 34.0 4 35.417765073348335 37.0 35.0 37.0 33.0 37.0 5 35.45738096870928 37.0 35.0 37.0 33.0 37.0 6 35.54124869855797 37.0 35.0 37.0 33.0 37.0 7 35.45803215730771 37.0 35.0 37.0 33.0 37.0 8 35.43774150711663 37.0 35.0 37.0 33.0 37.0 9 37.057081714973286 39.0 37.0 39.0 33.0 39.0 10-11 36.960284353393725 39.0 37.0 39.0 33.0 39.0 12-13 36.85496730029489 39.0 37.0 39.0 33.0 39.0 14-15 38.08995037375544 40.0 38.0 41.0 33.0 41.0 16-17 38.022438730914786 40.0 38.0 41.0 33.0 41.0 18-19 37.95060895018049 40.0 38.0 41.0 33.0 41.0 20-21 37.843161740409855 40.0 38.0 41.0 32.0 41.0 22-23 37.76694602210737 40.0 38.0 41.0 32.0 41.0 24-25 37.78688411399074 40.0 38.0 41.0 32.0 41.0 26-27 37.70513126640441 40.0 38.0 41.0 32.0 41.0 28-29 37.572656780902626 40.0 37.5 41.0 32.0 41.0 30-31 37.413933378637026 40.0 37.0 41.0 31.5 41.0 32-33 37.223948091945715 40.0 37.0 41.0 31.0 41.0 34-35 36.930666338318815 40.0 36.5 41.0 30.5 41.0 36-37 36.686931184109625 40.0 36.0 41.0 30.0 41.0 38-39 36.371582272956815 39.5 35.5 41.0 30.0 41.0 40-41 36.25154240363095 39.0 35.0 41.0 30.0 41.0 42-43 36.09830329366143 39.0 35.0 41.0 29.0 41.0 44-45 36.08852252819531 39.0 35.0 41.0 29.0 41.0 46-47 35.92795357202975 39.0 35.0 41.0 28.0 41.0 48-49 35.740165462378194 39.0 35.0 41.0 27.0 41.0 50-51 34.60320840528931 38.0 34.0 40.0 25.5 40.5 52-53 34.72386127719001 38.5 34.5 39.5 25.5 40.5 54-55 35.229292576637015 39.0 35.0 40.5 26.0 41.0 56-57 35.122962892536734 39.0 35.0 41.0 26.0 41.0 58-59 34.91968590826626 38.5 34.5 40.5 26.0 41.0 60-61 34.62319684037931 38.0 34.0 40.0 25.0 41.0 62-63 34.267818222058736 38.0 34.0 40.0 23.5 41.0 64-65 33.89170521636669 37.0 34.0 40.0 23.0 41.0 66-67 33.53184698782411 36.5 33.0 39.5 22.0 41.0 68-69 33.12226214003828 36.0 33.0 39.0 22.0 41.0 70-71 32.71119489523002 35.5 32.5 39.0 20.5 40.5 72-73 32.27634866800915 35.0 32.0 38.0 19.5 40.0 74-75 31.82785257389385 35.0 32.0 37.0 18.0 39.5 76-77 31.385094981888916 35.0 31.0 37.0 16.0 39.0 78-79 30.95921436542622 35.0 31.0 36.0 12.0 39.0 80-81 30.510816619804363 34.5 31.0 36.0 7.5 37.5 82-83 30.08018706475726 34.0 30.0 35.0 6.0 37.0 84-85 29.66381476817187 34.0 30.0 35.0 2.0 36.5 86-87 29.33846220986415 34.0 29.5 35.0 2.0 36.0 88-89 28.939362464853275 34.0 29.0 35.0 2.0 36.0 90-91 28.554109907169 34.0 29.0 35.0 2.0 35.5 92-93 28.150493768664393 34.0 29.0 35.0 2.0 35.0 94-95 27.688106534330014 34.0 27.0 35.0 2.0 35.0 96-97 27.122296117806222 34.0 26.5 35.0 2.0 35.0 98-99 26.39509239459099 33.0 25.0 35.0 2.0 35.0 100-101 23.982171335232763 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25069359891012155 1101 2 0.09747944041078327 1101 3 0.3113883633776737 1101 4 0.3344724261193974 1101 5 0.2924213116197336 1101 6 0.3267876089748398 1101 7 0.330756788469877 1101 8 0.33150697231091186 1101 9 0.4297533195887553 1101 10-11 0.4513426351451102 1101 12-13 0.374079359574786 1101 14-15 0.3573866326568762 1101 16-17 0.3664893036075938 1101 18-19 0.4822850395326981 1101 20-21 0.5363636766750091 1101 22-23 0.48420174279409167 1101 24-25 0.3762093311317045 1101 26-27 0.3412806199421681 1101 28-29 0.4344790778337213 1101 30-31 0.49488311850846856 1101 32-33 0.4723148645748907 1101 34-35 0.4799533781204488 1101 36-37 0.40924107246565455 1101 38-39 0.5708041565125654 1101 40-41 0.42280935514316553 1101 42-43 0.41828054227674016 1101 44-45 0.45862630819162575 1101 46-47 0.34767634611396403 1101 48-49 0.3133238395525524 1101 50-51 0.31807358502967986 1101 52-53 0.4192935560992197 1101 54-55 0.5273088073083301 1101 56-57 0.4361466676009158 1101 58-59 0.32910959257548456 1101 60-61 0.40292875377804904 1101 62-63 0.6032431066881259 1101 64-65 0.5029161250369683 1101 66-67 0.45592638226711557 1101 68-69 0.34165117949537205 1101 70-71 0.3574757563151181 1101 72-73 0.4289206619666075 1101 74-75 0.412208040379749 1101 76-77 0.3637066987524129 1101 78-79 0.2710446421381185 1101 80-81 0.1727654643251455 1101 82-83 0.06021132708239563 1101 84-85 -0.1478791011037579 1101 86-87 -0.05271670004155027 1101 88-89 -0.09471816030531954 1101 90-91 0.016498926478728748 1101 92-93 -0.02281711631758654 1101 94-95 -0.06409486971547906 1101 96-97 -0.018691448671308564 1101 98-99 -0.0017558578453353846 1101 100-101 -0.15930577888781983 1102 1 0.2911181335258455 1102 2 0.32261998631706845 1102 3 0.33612629924340354 1102 4 0.300855965492282 1102 5 0.3296494893885651 1102 6 0.3133141075205259 1102 7 0.34161746482934063 1102 8 0.40528026106558457 1102 9 0.37032101366862946 1102 10-11 0.42578668009244325 1102 12-13 0.37263353691958656 1102 14-15 0.4421485888292196 1102 16-17 0.4299056365367733 1102 18-19 0.46173227692875685 1102 20-21 0.530792707098783 1102 22-23 0.40467496286347426 1102 24-25 0.5200260928797746 1102 26-27 0.4711105725854736 1102 28-29 0.5749067023099173 1102 30-31 0.5007388926149972 1102 32-33 0.5132689499810752 1102 34-35 0.4528690412948819 1102 36-37 0.5047400495142185 1102 38-39 0.6523503373473147 1102 40-41 0.7149377096569012 1102 42-43 0.6652287249502677 1102 44-45 0.7505918649185332 1102 46-47 0.66150681804924 1102 48-49 0.6005131803399806 1102 50-51 0.4609642435387826 1102 52-53 0.5344417863541508 1102 54-55 0.49838551498750405 1102 56-57 0.4938777183293439 1102 58-59 0.5639610936370403 1102 60-61 0.45164602201897 1102 62-63 0.5173545727328843 1102 64-65 0.45721489036291985 1102 66-67 0.44206548802974055 1102 68-69 0.5003815252959427 1102 70-71 0.4359667002149692 1102 72-73 0.33547467126208375 1102 74-75 0.2050542073406625 1102 76-77 0.14638740795966143 1102 78-79 0.1641295466186854 1102 80-81 0.13839430833140298 1102 82-83 0.10284932538040792 1102 84-85 0.17825872474828586 1102 86-87 0.016206606934741075 1102 88-89 -0.09749140167398451 1102 90-91 -0.16254572530254663 1102 92-93 0.02673081144855871 1102 94-95 -0.1146462082967723 1102 96-97 -0.28527685485727616 1102 98-99 -0.3770803542591423 1102 100-101 -0.5338399183042775 1103 1 0.3164120081098254 1103 2 0.4260349613636656 1103 3 0.32835040232106394 1103 4 0.3306743019688412 1103 5 0.36213152383025005 1103 6 0.23085505727748057 1103 7 0.28451704191524385 1103 8 0.3290172618007361 1103 9 0.43041845989299077 1103 10-11 0.4022342307321196 1103 12-13 0.343313857095886 1103 14-15 0.46985229430807607 1103 16-17 0.5178367173460146 1103 18-19 0.6259143747191942 1103 20-21 0.5715020921800829 1103 22-23 0.5079620058513044 1103 24-25 0.5425381411264993 1103 26-27 0.49810018237212006 1103 28-29 0.5750713074186322 1103 30-31 0.5409893804391785 1103 32-33 0.5003019404758575 1103 34-35 0.43630510758135443 1103 36-37 0.5455673209013199 1103 38-39 0.6660883886503726 1103 40-41 0.6286770076015813 1103 42-43 0.5339468311975395 1103 44-45 0.5915301358241649 1103 46-47 0.5570161607205293 1103 48-49 0.4235033217042954 1103 50-51 0.4608910789886238 1103 52-53 0.4699528331724423 1103 54-55 0.5569921351629077 1103 56-57 0.5701738806822618 1103 58-59 0.522689691039325 1103 60-61 0.5990210304610386 1103 62-63 0.4396175105128748 1103 64-65 0.481557383728493 1103 66-67 0.42776989743749994 1103 68-69 0.4104745780162986 1103 70-71 0.3777242083260077 1103 72-73 0.3549743855027714 1103 74-75 0.23577248593916167 1103 76-77 0.46314325186463634 1103 78-79 0.3143370337788802 1103 80-81 0.22346210748121464 1103 82-83 0.23105025932074952 1103 84-85 0.18959021305200352 1103 86-87 0.23763939031756465 1103 88-89 0.15497077606612564 1103 90-91 0.27666790767986527 1103 92-93 0.277549479798072 1103 94-95 0.2055109097001555 1103 96-97 0.08062844056168572 1103 98-99 -0.019382075907547147 1103 100-101 -0.23264506676477126 1104 1 0.2894640197487881 1104 2 0.2922500118178455 1104 3 0.34579230074122336 1104 4 0.373757602660838 1104 5 0.28849429964954965 1104 6 0.3065549808034902 1104 7 0.36383572395131836 1104 8 0.35771622473883014 1104 9 0.3756164529170718 1104 10-11 0.3460045149915345 1104 12-13 0.3519387475142537 1104 14-15 0.3580420757043825 1104 16-17 0.4138284777399477 1104 18-19 0.3986692336153581 1104 20-21 0.3932521807780063 1104 22-23 0.48752083486666464 1104 24-25 0.37102272042629636 1104 26-27 0.3666542177739771 1104 28-29 0.38731682156632274 1104 30-31 0.4818660101745067 1104 32-33 0.4784138256529644 1104 34-35 0.4764404292384157 1104 36-37 0.551775703196661 1104 38-39 0.6673057939438678 1104 40-41 0.47103170702150265 1104 42-43 0.4303086044614872 1104 44-45 0.5752020064525638 1104 46-47 0.6486206922707041 1104 48-49 0.5836124603460391 1104 50-51 0.650231700666204 1104 52-53 0.6963638715400009 1104 54-55 0.7613259721054391 1104 56-57 0.768490170574637 1104 58-59 0.7575213783166603 1104 60-61 0.7685243458882596 1104 62-63 0.6763271238529285 1104 64-65 0.7758632190669346 1104 66-67 0.7160539889824662 1104 68-69 0.5905807323072381 1104 70-71 0.5741836724485196 1104 72-73 0.5921603292104507 1104 74-75 0.6161802228686462 1104 76-77 0.6358962455415416 1104 78-79 0.6734692638350239 1104 80-81 0.5481404737356712 1104 82-83 0.42155524411921164 1104 84-85 0.44183990062777667 1104 86-87 0.4245672343106257 1104 88-89 0.4017952402818956 1104 90-91 0.37661241740698514 1104 92-93 0.47442418835755973 1104 94-95 0.45764297188656755 1104 96-97 0.47727990569141454 1104 98-99 0.38641840625163226 1104 100-101 0.37049829398728207 1105 1 0.3306647512598282 1105 2 0.3195244550466221 1105 3 0.3539450545321472 1105 4 0.314388338783381 1105 5 0.36270932682124624 1105 6 0.34466770041503736 1105 7 0.3864568437328586 1105 8 0.3880582219719315 1105 9 0.4749771719499236 1105 10-11 0.5017197840411072 1105 12-13 0.5054065867988413 1105 14-15 0.5718110868533657 1105 16-17 0.5481459187172248 1105 18-19 0.5665340057980544 1105 20-21 0.6389030131091573 1105 22-23 0.6163053194181245 1105 24-25 0.5709547346586561 1105 26-27 0.5722387710458889 1105 28-29 0.6135400567620337 1105 30-31 0.6105645376581634 1105 32-33 0.5074367972696123 1105 34-35 0.6275890100708352 1105 36-37 0.6364343163096109 1105 38-39 0.7539261609652854 1105 40-41 0.7566066303891645 1105 42-43 0.631614123346047 1105 44-45 0.6456282540450857 1105 46-47 0.6266996054930658 1105 48-49 0.5597609846649405 1105 50-51 0.4881080242129059 1105 52-53 0.4384965110379824 1105 54-55 0.48292950939983825 1105 56-57 0.5190670040993695 1105 58-59 0.6353083426814976 1105 60-61 0.6460667197274788 1105 62-63 0.6187695704815539 1105 64-65 0.5300859659858119 1105 66-67 0.5906909461135683 1105 68-69 0.6159154619013307 1105 70-71 0.4823703908664996 1105 72-73 0.6581500392282678 1105 74-75 0.7744040706709434 1105 76-77 0.6228241591527102 1105 78-79 0.5491731397584552 1105 80-81 0.5962799920596176 1105 82-83 0.5201178226730043 1105 84-85 0.539286280597576 1105 86-87 0.5009078480926945 1105 88-89 0.6345201867070749 1105 90-91 0.61623585650349 1105 92-93 0.507738514309473 1105 94-95 0.44232164730001955 1105 96-97 0.4408203196416629 1105 98-99 0.4405781971063867 1105 100-101 0.2436993555372915 1106 1 0.1054296105025152 1106 2 0.1982487256555423 1106 3 0.10910892571038744 1106 4 0.2563036754611474 1106 5 0.23452789843738486 1106 6 0.17637068960225122 1106 7 0.22015421649862787 1106 8 0.2762946630206571 1106 9 0.3353771914913679 1106 10-11 0.2605647972424947 1106 12-13 0.31909425863162966 1106 14-15 0.40015309970099366 1106 16-17 0.38893617180950457 1106 18-19 0.36040172066564224 1106 20-21 0.43762610163497584 1106 22-23 0.4826224064512843 1106 24-25 0.38271688688740824 1106 26-27 0.45263813193586344 1106 28-29 0.47048989848939726 1106 30-31 0.39047691084049774 1106 32-33 0.4852133076643099 1106 34-35 0.4895240474067819 1106 36-37 0.3077642863038861 1106 38-39 0.5213508184338025 1106 40-41 0.6396691678199815 1106 42-43 0.5350363520917725 1106 44-45 0.5187060058887667 1106 46-47 0.3985825919148098 1106 48-49 0.4853956891419884 1106 50-51 0.4986281822194627 1106 52-53 0.48013091759519 1106 54-55 0.4942675838606121 1106 56-57 0.6237222523957087 1106 58-59 0.6266730838031194 1106 60-61 0.5399885007210656 1106 62-63 0.5284180185285692 1106 64-65 0.3575704233376982 1106 66-67 0.2800775627517993 1106 68-69 0.333543178129851 1106 70-71 0.42355843019192463 1106 72-73 0.3785736982509391 1106 74-75 0.4732875909929639 1106 76-77 0.5536419818254146 1106 78-79 0.5312921698570854 1106 80-81 0.5490454122465387 1106 82-83 0.5260733296754303 1106 84-85 0.356148456249894 1106 86-87 0.4030623363226802 1106 88-89 0.48599482448148024 1106 90-91 0.4128240527711604 1106 92-93 0.44395689740298394 1106 94-95 0.5212932502614223 1106 96-97 0.48988958186041387 1106 98-99 0.582001628000107 1106 100-101 0.5609040517659238 1107 1 0.11545854269716216 1107 2 0.1573750258449671 1107 3 0.10436923842627266 1107 4 0.090684454456742 1107 5 0.056065643749953153 1107 6 -0.024614579047003815 1107 7 0.021200718297308185 1107 8 0.0886534738691509 1107 9 0.17417316996628074 1107 10-11 0.14449935085262666 1107 12-13 0.182661867232504 1107 14-15 0.22763208450909644 1107 16-17 0.21253436783636914 1107 18-19 0.34745583361363686 1107 20-21 0.2766133165864346 1107 22-23 0.1858456727994522 1107 24-25 0.15037107428958052 1107 26-27 0.19538769814032975 1107 28-29 0.141410960818952 1107 30-31 0.1750483433380765 1107 32-33 0.1146949766538512 1107 34-35 0.20049478541509558 1107 36-37 0.21533065067048085 1107 38-39 0.3938040524227233 1107 40-41 0.23572921753105192 1107 42-43 0.23832494282157057 1107 44-45 0.14631174602285313 1107 46-47 0.18079302949134757 1107 48-49 0.21162661910717162 1107 50-51 0.030880260836084972 1107 52-53 0.1260282133011259 1107 54-55 0.2636583587878789 1107 56-57 0.29791869483509004 1107 58-59 0.23672374221042247 1107 60-61 0.08045047474788447 1107 62-63 0.23005283535171372 1107 64-65 0.2806108064019526 1107 66-67 0.01918031076895943 1107 68-69 0.09157261168261499 1107 70-71 0.09267492599845895 1107 72-73 0.036802831821653115 1107 74-75 0.033791814019039634 1107 76-77 0.008054620380772093 1107 78-79 0.12025710685210811 1107 80-81 0.057595671108064295 1107 82-83 0.11149903852044574 1107 84-85 -0.041555331877166424 1107 86-87 0.0449610511552585 1107 88-89 0.23084778372281178 1107 90-91 0.22419435737953464 1107 92-93 0.24459880444602433 1107 94-95 0.13211603765434532 1107 96-97 0.18537484988504715 1107 98-99 0.08739086345812197 1107 100-101 0.15257357431392293 1108 1 0.1644404496670795 1108 2 0.23067710169842215 1108 3 0.1301810499555529 1108 4 0.26233396972558154 1108 5 0.26218223176771005 1108 6 0.09788119073435553 1108 7 0.14929298353648335 1108 8 0.24696041078534137 1108 9 0.3056604504029252 1108 10-11 0.23137927929071367 1108 12-13 0.21053789899131203 1108 14-15 0.271300246666037 1108 16-17 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-1.1528644629731346 2316 86-87 -1.0177582495086703 2316 88-89 -1.2272481937731357 2316 90-91 -1.0955069513820597 2316 92-93 -1.1644533212700736 2316 94-95 -1.1399287479265219 2316 96-97 -0.974878391487497 2316 98-99 -1.0762262259482078 2316 100-101 -0.683041647654953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11521.0 3 3462.0 4 3432.0 5 3828.0 6 4116.0 7 5157.0 8 6132.0 9 7383.0 10 9086.0 11 10924.0 12 13263.0 13 17791.0 14 16541.0 15 17223.0 16 20327.0 17 26029.0 18 22851.0 19 18193.0 20 18423.0 21 19843.0 22 21339.0 23 23839.0 24 26734.0 25 30900.0 26 35438.0 27 41590.0 28 49046.0 29 58146.0 30 69763.0 31 85241.0 32 106816.0 33 137311.0 34 186008.0 35 267741.0 36 420560.0 37 662036.0 38 626558.0 39 103346.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26706993839639 18.614029817433632 14.671736582764433 23.447163661405547 2 15.108730420020688 19.77565817908338 40.54635924337296 24.56925215752297 3 19.175099753600414 23.877976808165673 29.7457199970663 27.201203441167614 4 12.93708388939588 16.41474796477986 36.99609497402683 33.652073171797426 5 14.299710694803775 36.832805692342696 33.946745246315174 14.920738366538352 6 32.29957565729453 35.50040781189813 17.59395658635709 14.60605994445025 7 28.835227317035432 32.23419788742653 20.612735518440804 18.317839277097235 8 26.187322581651433 35.244034064322925 19.720029946657515 18.848613407368127 9 26.240716403980425 17.11497543974354 20.333871006482333 36.3104371497937 10-11 25.261062929594264 26.342604896173714 27.095818002378092 21.300514171853926 12-13 25.82432493554661 23.686874279627112 27.663472981045324 22.825327803780954 14-15 22.843340783574472 25.274705222639316 25.855331195736408 26.026622798049804 16-17 22.608950208895184 28.29597113334918 26.470703830483288 22.624374827272348 18-19 22.438395340393118 27.07738311366651 28.530172526736212 21.95404901920416 20-21 23.203118139510508 26.262938371386962 28.406261547068993 22.12768194203354 22-23 22.88931796159061 26.045201758802904 28.36570409208071 22.699776187525778 24-25 22.71784386100962 26.35606164488809 28.471409389942497 22.454685104159793 26-27 22.5842860519191 26.482719243576412 28.46547631769229 22.467518386812195 28-29 22.402714397093046 26.67909720183485 28.58618547714567 22.332002923926435 30-31 22.57355029787935 26.45080962379678 28.330804014527324 22.64483606379655 32-33 22.43640285716291 26.477483737583356 28.39762261834851 22.688490786905216 34-35 22.929095520308497 26.534087367162783 28.08045874470772 22.456358367821004 36-37 23.00599965398043 26.274304668041776 28.2214938221741 22.498201855803696 38-39 22.65889058357999 26.58277554746837 28.215965679142897 22.54236818980874 40-41 22.719878365159698 26.468930829253406 28.29134258645405 22.519848219132847 42-43 22.625112976285262 26.423540293219887 27.948444496993375 23.002902233501473 44-45 22.73731250601224 26.635232250490954 27.840480635299507 22.786974608197298 46-47 22.831110226790656 26.82545954421623 27.595115547730153 22.74831468126296 48-49 22.948517184143874 26.700556769703216 27.614400608238437 22.736525437914466 50-51 22.746223539434343 26.904905746089327 27.575960176615993 22.77291053786034 52-53 22.914557457059725 26.947361011169164 27.32135594280887 22.81672558896224 54-55 22.66770815036593 26.777324831080847 27.40277812752847 23.152188891024757 56-57 22.712765591467797 27.050626648560776 27.392098042024266 22.84450971794716 58-59 22.863312441280677 26.80718704956446 27.47026881156392 22.859231697590936 60-61 22.841709275828403 26.82690118232512 27.71333636417483 22.618053177671648 62-63 22.61495997023514 26.807673366001904 27.786620107201664 22.790746556561288 64-65 22.506460958912683 26.696212908151136 27.662212307422752 23.135113825513436 66-67 22.54198807434495 26.894395342630258 27.427739397809802 23.135877185214994 68-69 22.44952306981473 26.83456140463423 27.584171766527877 23.13174375902317 70-71 22.620614896342207 26.73020372309981 27.567380145533036 23.08180123502494 72-73 22.696744768587607 26.633963283080963 27.56157775339738 23.107714194934047 74-75 22.51705536487381 27.051286323464556 27.250848993981048 23.180809317680588 76-77 22.719281794063264 26.695605424711026 27.26830627239838 23.316806508827334 78-79 22.67205679969974 26.799480795696233 27.357531590141377 23.170930814462654 80-81 22.64622074025259 27.035090975938203 27.31433125347076 23.004357030338447 82-83 22.722967979573244 26.86339020031829 27.31310729912451 23.100534520983956 84-85 22.747275006706413 26.81954940729392 27.163903155494136 23.269272430505534 86-87 22.622740515478963 26.792333960371618 27.32696660336849 23.25795892078093 88-89 22.59126930742012 26.667641749392267 27.380995585534567 23.360093357653046 90-91 22.582293612720107 26.767024349333607 27.410289870038834 23.240392167907448 92-93 22.690398955731716 26.49574174042523 27.4435532032504 23.37030610059265 94-95 22.610466077768756 26.4185157538457 27.564665311843704 23.406352856541833 96-97 22.562329431358062 26.396053775847182 27.63337955369965 23.408237239095104 98-99 22.591017261547815 26.43329296413535 27.285692673326935 23.689997100989903 100-101 22.69014806664954 25.9104279827229 27.801733779098488 23.59769017152907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2085.0 1 1167.5 2 269.5 3 295.5 4 281.0 5 309.5 6 525.5 7 1079.0 8 1772.0 9 2217.5 10 2315.0 11 2368.5 12 2689.0 13 3037.0 14 3383.5 15 3692.0 16 3959.5 17 4211.0 18 4389.0 19 4647.5 20 5033.0 21 5605.0 22 6511.0 23 8141.5 24 10061.5 25 12403.0 26 15868.0 27 20117.0 28 24038.0 29 28434.0 30 33604.0 31 38749.5 32 44758.0 33 50541.5 34 56260.0 35 61815.0 36 67909.0 37 75089.0 38 81034.0 39 85462.5 40 90157.0 41 96402.0 42 102058.5 43 106707.0 44 110616.5 45 115381.0 46 117878.5 47 122627.5 48 135140.5 49 137022.5 50 127943.5 51 122737.0 52 120093.5 53 117876.5 54 115569.5 55 108767.0 56 96196.0 57 85767.0 58 77255.0 59 65708.5 60 54943.5 61 46367.5 62 38288.0 63 29450.0 64 21715.0 65 16250.0 66 12482.5 67 10549.5 68 8306.5 69 5624.0 70 4055.0 71 3133.0 72 2565.0 73 2078.5 74 1544.0 75 1229.0 76 863.0 77 515.0 78 322.0 79 192.0 80 126.5 81 99.5 82 70.0 83 40.0 84 32.0 85 29.5 86 22.5 87 21.5 88 18.5 89 10.0 90 7.0 91 8.0 92 6.5 93 5.0 94 5.0 95 4.5 96 4.0 97 3.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27989575994862814 2 0.1089470632609929 3 0.11954563307751294 4 0.1216965193049832 5 0.12771276628906664 6 0.0575128273867044 7 0.0663034058815828 8 0.07107276229901682 9 0.06829843078822187 10-11 0.0638407970124502 12-13 0.0677996745615621 14-15 0.06370052182370214 16-17 0.06440189776744244 18-19 0.06907773739237777 20-21 0.07327040692273643 22-23 0.0816089875872044 24-25 0.08979170693084121 26-27 0.09032163542166721 28-29 0.08419628551300196 30-31 0.08015947730347446 32-33 0.07333275145106889 34-35 0.2394497471929376 36-37 0.36250225998915203 38-39 0.33480570327745185 40-41 0.3183311616655964 42-43 0.3416324291298574 44-45 0.35466243555134386 46-47 0.36010199564835194 48-49 0.3698589143323836 50-51 0.363686806027469 52-53 0.36409204546163004 54-55 0.35095293611556183 56-57 0.3271217401604748 58-59 0.3127669125119234 60-61 0.26959332664168734 62-63 0.3404478830915405 64-65 0.36970305301155243 66-67 0.37447240942898646 68-69 0.3861152500950754 70-71 0.3870660041521456 72-73 0.3702797398986278 74-75 0.3738022057494124 76-77 0.3572185612129751 78-79 0.33603700771201817 80-81 0.3345874974282882 82-83 0.3488955666805903 84-85 0.3557534647971621 86-87 0.34930080611475134 88-89 0.36378032281996775 90-91 0.38684779830298194 92-93 0.3818446499043011 94-95 0.3814394104701401 96-97 0.3871283486804781 98-99 0.37598426424104886 100-101 0.3547091939475932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3207980.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.937976343717914 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29577209169716 36.826003285576924 2 14.672828043987082 14.948083356001096 3 5.316223327197687 8.123929742361515 4 2.5775316647788267 5.25176987862751 5 1.3880609160703388 3.535250705321517 6 0.8652115812508601 2.6443276234840245 7 0.5808039286033115 2.0709483742873744 8 0.3899155699482684 1.588920806245775 9 0.2980461151367668 1.3663679365956292 >10 1.4653635195200867 13.25945311436954 >50 0.09607765325707285 3.3508551177537234 >100 0.050795861592755794 4.904698899932636 >500 0.002328174991033618 0.8055241054463912 >1k 9.190164430544502E-4 0.8829837472587723 >5k 1.2253552574059334E-4 0.4408833067375208 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7612 0.23728327483338424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6515 0.20308730104302394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4240 0.13217040006483832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4118 0.1283673838365576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.117226416623545E-5 0.0 5 0.0 0.0 0.0 3.117226416623545E-5 0.0 6 0.0 0.0 0.0 3.117226416623545E-5 0.0 7 0.0 0.0 0.0 3.117226416623545E-5 0.0 8 0.0 0.0 0.0 3.117226416623545E-5 0.0 9 0.0 0.0 0.0 3.117226416623545E-5 0.0 10-11 0.0 0.0 0.0 3.117226416623545E-5 0.0 12-13 0.0 0.0 0.0 3.117226416623545E-5 0.0 14-15 0.0 0.0 0.0 3.117226416623545E-5 0.0 16-17 0.0 0.0 0.0 3.117226416623545E-5 0.0 18-19 0.0 0.0 0.0 3.117226416623545E-5 0.0 20-21 0.0 0.0 0.0 3.117226416623545E-5 0.0 22-23 0.0 0.0 0.0 1.4027518874805953E-4 0.0 24-25 0.0 0.0 0.0 3.740671699948254E-4 0.0 26-27 0.0 0.0 0.0 6.078591512415913E-4 0.0 28-29 0.0 0.0 0.0 0.0017612329253923029 0.0 30-31 0.0 0.0 0.0 0.005361629436592497 0.0 32-33 0.0 0.0 0.0 0.013061178685652652 0.0 34-35 0.0 0.0 0.0 0.024018229540084417 0.0 36-37 0.0 0.0 0.0 0.04399965087064134 0.0 38-39 0.0 0.0 0.0 0.07328599305481955 0.0 40-41 0.0 0.0 0.0 0.10643769599561095 0.0 42-43 0.0 0.0 0.0 0.1430651063909376 0.0 44-45 0.0 0.0 0.0 0.18867012886614004 0.0 46-47 0.0 0.0 0.0 0.24742984681949387 0.0 48-49 0.0 0.0 0.0 0.3125642927948429 0.0 50-51 0.0 0.0 0.0 0.3823745783951271 0.0 52-53 0.0 0.0 0.0 0.4555202962611986 0.0 54-55 0.0 0.0 0.0 0.5372383867729849 0.0 56-57 0.0 0.0 0.0 0.638407970124502 0.0 58-59 0.0 0.0 0.0 0.7508307408400302 0.0 60-61 0.0 0.0 0.0 0.8756756588258031 0.0 62-63 0.0 0.0 0.0 1.002764979831545 0.0 64-65 0.0 0.0 0.0 1.1426349291454436 0.0 66-67 0.0 0.0 0.0 1.3099676431897955 0.0 68-69 0.0 0.0 0.0 1.4983572216784393 0.0 70-71 0.0 0.0 0.0 1.70917524423469 0.0 72-73 0.0 0.0 0.0 1.9246691064158754 0.0 74-75 0.0 0.0 0.0 2.152320151621893 0.0 76-77 0.0 0.0 0.0 2.408789955049595 0.0 78-79 0.0 0.0 0.0 2.7001882804755644 0.0 80-81 0.0 0.0 0.0 3.0190493706319863 0.0 82-83 0.0 0.0 0.0 3.352109427116129 0.0 84-85 0.0 0.0 0.0 3.692354690490589 0.0 86-87 0.0 0.0 0.0 4.0614498843509 0.0 88-89 0.0 0.0 0.0 4.46003404011247 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11380 0.0 27.588062 1 GTATCAA 15705 0.0 25.918203 1 TCAACGC 18000 0.0 22.367222 4 ATCAACG 17945 0.0 22.356499 3 CAACGCA 18070 0.0 22.22809 5 GTACATG 11180 0.0 22.133902 1 GAGTACT 8590 0.0 21.902979 12-13 TATCAAC 18505 0.0 21.8337 2 AACGCAG 18570 0.0 21.716827 6 TACATGG 11680 0.0 20.828182 2 GTACTTT 8920 0.0 20.774534 14-15 ACATGGG 11660 0.0 20.294523 3 ACGCAGA 20495 0.0 19.58458 7 AGTACTT 9040 0.0 19.318565 12-13 CGCAGAG 20775 0.0 19.229382 8 AGAGTAC 15305 0.0 18.902866 10-11 GCAGAGT 21825 0.0 17.804295 9 GAGTACA 10135 0.0 17.01159 1 CATGGGG 7085 0.0 16.800056 4 AGTACAT 9115 0.0 16.752363 2 >>END_MODULE