FastQCFastQC Report
Fri 27 May 2016
SRR939322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207980
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78080.2433930386099664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.22453381878939394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46900.14619791893964426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.1395582266722361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126350.025.7234271
GTATCAA168850.024.180741
GTACATG114050.023.4907281
TACATGG116450.022.53492
TAGATCG1302.9625717E-421.9017075
ACATGGG117600.021.4671463
GAGTACT95450.021.15537612-13
ATCAACG194400.020.6999663
CAACGCA195150.020.6447285
TCAACGC195150.020.620414
GTACTTT99300.020.28736114-15
AACGCAG199550.020.189526
TATCAAC201750.020.0164012
AGTACTT101650.019.04802112-13
AGAGTAC166800.018.9624510-11
TGCGCCG1801.2434556E-418.4542145
ACGCAGA221100.018.114397
CGCAGAG221100.018.0070768
CATGGGG72350.017.709054
TACTTTT113000.017.07179614-15
Produced by FastQC (version 0.11.5)