##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3207980 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28356660577684 33.0 31.0 34.0 31.0 34.0 2 32.48713084246161 34.0 31.0 34.0 31.0 34.0 3 32.574685939438524 34.0 31.0 34.0 31.0 34.0 4 36.11004152145587 37.0 35.0 37.0 35.0 37.0 5 36.041875884513 37.0 35.0 37.0 35.0 37.0 6 36.07901015592367 37.0 35.0 37.0 35.0 37.0 7 36.024168479853365 37.0 35.0 37.0 35.0 37.0 8 36.004236934145474 37.0 35.0 37.0 35.0 37.0 9 37.711763789051055 39.0 37.0 39.0 35.0 39.0 10-11 37.63122229565022 39.0 37.0 39.0 35.0 39.0 12-13 37.5513243536431 39.0 37.0 39.0 35.0 39.0 14-15 38.858976832773266 40.0 38.0 41.0 35.0 41.0 16-17 38.80276825291928 40.0 38.0 41.0 35.0 41.0 18-19 38.90765466118866 40.0 38.0 41.0 35.0 41.0 20-21 38.91023369846445 40.0 38.0 41.0 35.0 41.0 22-23 38.85139620571201 40.0 38.0 41.0 35.0 41.0 24-25 38.7878079040393 40.0 38.0 41.0 34.0 41.0 26-27 38.62638856850728 40.0 38.0 41.0 34.0 41.0 28-29 38.48154009688339 40.0 38.0 41.0 34.0 41.0 30-31 38.30913082375825 40.0 38.0 41.0 34.0 41.0 32-33 38.126311573014796 40.0 38.0 41.0 33.0 41.0 34-35 38.09176382022332 40.0 38.0 41.0 33.0 41.0 36-37 38.01756525913503 40.0 38.0 41.0 33.0 41.0 38-39 37.921874824656015 40.0 37.0 41.0 33.0 41.0 40-41 37.76258065823353 40.0 37.0 41.0 33.0 41.0 42-43 37.60319765085817 40.0 37.0 41.0 33.0 41.0 44-45 37.39362698645253 40.0 37.0 41.0 32.0 41.0 46-47 37.1341813228262 40.0 37.0 41.0 31.0 41.0 48-49 36.844600028678485 40.0 36.0 41.0 31.0 41.0 50-51 36.60422119215207 39.0 36.0 41.0 30.0 41.0 52-53 36.378974931265155 39.0 35.5 41.0 30.0 41.0 54-55 36.1041734985879 39.0 35.0 40.5 29.5 41.0 56-57 35.85948182345277 39.0 35.0 40.0 29.0 41.0 58-59 35.56470224253268 38.0 35.0 40.0 28.0 41.0 60-61 35.222853945473474 38.0 34.0 40.0 28.0 41.0 62-63 34.84148545190432 37.5 34.0 40.0 27.5 41.0 64-65 34.4239959413712 37.0 34.0 39.5 26.0 41.0 66-67 34.047341941034546 36.0 33.5 39.0 26.0 40.5 68-69 33.65671279122688 36.0 33.0 39.0 26.0 40.0 70-71 33.250964313991986 35.0 33.0 38.5 25.5 40.0 72-73 32.7523134495851 35.0 32.0 37.5 24.5 40.0 74-75 32.242493251204806 35.0 32.0 37.0 23.0 39.0 76-77 30.251247514011933 33.5 29.5 35.0 20.0 37.5 78-79 31.24280279178798 34.0 31.0 36.0 21.0 38.0 80-81 31.291547952293968 34.0 31.0 36.0 21.0 37.0 82-83 30.98159153111927 34.0 31.0 35.0 20.0 37.0 84-85 30.605395918927176 34.0 31.0 35.0 19.0 36.5 86-87 30.06849684224964 34.0 30.0 35.0 15.5 36.0 88-89 29.569540022069965 34.0 30.0 35.0 7.0 36.0 90-91 29.144911439597504 34.0 29.0 35.0 4.5 35.0 92-93 28.794236871800948 34.0 29.0 35.0 2.0 35.0 94-95 28.38704215737006 34.0 29.0 35.0 2.0 35.0 96-97 27.8205676157582 33.5 28.5 35.0 2.0 35.0 98-99 27.047902106621613 33.0 27.0 35.0 2.0 35.0 100-101 23.639132569405046 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18489745244825428 1101 2 0.18276682608235006 1101 3 0.20792692226151388 1101 4 0.15145840493143226 1101 5 0.1494623027755253 1101 6 0.1533877408820814 1101 7 0.19954834256023446 1101 8 0.2048637608900279 1101 9 0.24116257494096516 1101 10-11 0.2487473864067269 1101 12-13 0.23616560139104337 1101 14-15 0.2790006805694887 1101 16-17 0.25571865192666365 1101 18-19 0.26143621485574187 1101 20-21 0.2492131677316749 1101 22-23 0.25258372786922934 1101 24-25 0.29393910256818145 1101 26-27 0.3399046206613079 1101 28-29 0.35897419588949475 1101 30-31 0.3487137234730042 1101 32-33 0.38558155056527 1101 34-35 0.3690419519078034 1101 36-37 0.3690165481050016 1101 38-39 0.44651092149399574 1101 40-41 0.5097867622610508 1101 42-43 0.4731222153992789 1101 44-45 0.5536516704494687 1101 46-47 0.45772519310181536 1101 48-49 0.48536003623466684 1101 50-51 0.5559594337474252 1101 52-53 0.5211543880978624 1101 54-55 0.4164094410362722 1101 56-57 0.446081175480991 1101 58-59 0.41751149685478595 1101 60-61 0.34710792398873735 1101 62-63 0.421508681606376 1101 64-65 0.36060571935603747 1101 66-67 0.3714036519398718 1101 68-69 0.433990106930473 1101 70-71 0.4047259471292435 1101 72-73 0.40465101130776304 1101 74-75 0.282841171461655 1101 76-77 0.41269389464182993 1101 78-79 0.5217339893391291 1101 80-81 0.48914036715060405 1101 82-83 0.5125835541618535 1101 84-85 0.48995164260007584 1101 86-87 0.5057249798402665 1101 88-89 0.5865858199524219 1101 90-91 0.5855499407128804 1101 92-93 0.5421738240130445 1101 94-95 0.9171598596144506 1101 96-97 0.5800973717451399 1101 98-99 0.42978757213227325 1101 100-101 0.34673786342369795 1102 1 0.1267219747533872 1102 2 0.18713061281994214 1102 3 0.20152883489735984 1102 4 0.09557931642317641 1102 5 0.16039711136050272 1102 6 0.17378326256288545 1102 7 0.16584822365556562 1102 8 0.1696118725334017 1102 9 0.23703498544248447 1102 10-11 0.17730999552886573 1102 12-13 0.25823373904298563 1102 14-15 0.27526347601143897 1102 16-17 0.28126337112307453 1102 18-19 0.2516316479198295 1102 20-21 0.23042077069364808 1102 22-23 0.1706011195091648 1102 24-25 0.22157371545385018 1102 26-27 0.2983005043320972 1102 28-29 0.26476312886418896 1102 30-31 0.26345854984970885 1102 32-33 0.23209645663839495 1102 34-35 0.310478837172127 1102 36-37 0.33044550232034453 1102 38-39 0.3378297814160902 1102 40-41 0.45643922208309107 1102 42-43 0.33494713584453706 1102 44-45 0.39207938578954327 1102 46-47 0.31828430884970516 1102 48-49 0.19790140128372968 1102 50-51 0.20145461258805852 1102 52-53 0.2572100187762416 1102 54-55 0.15936529193743354 1102 56-57 0.14343239564612986 1102 58-59 0.086361746339648 1102 60-61 -0.030289160453122577 1102 62-63 -0.09176984110324327 1102 64-65 0.0802885091507548 1102 66-67 -0.04741118017200563 1102 68-69 0.06749363498934002 1102 70-71 0.012121009116974335 1102 72-73 0.14108383700836669 1102 74-75 0.14480525775693565 1102 76-77 0.26394514157761506 1102 78-79 0.3425170137189788 1102 80-81 0.3760126369596648 1102 82-83 0.27291809933927524 1102 84-85 0.2555092732608699 1102 86-87 0.2834128164885037 1102 88-89 0.4394180251406503 1102 90-91 0.48063739231320923 1102 92-93 0.4102762295610205 1102 94-95 0.35515918350901643 1102 96-97 0.10968577790473333 1102 98-99 0.23902380014785507 1102 100-101 0.38293539563885304 1103 1 0.1843603901000037 1103 2 0.1838435940387413 1103 3 0.14486643101887609 1103 4 0.14316193305860736 1103 5 0.09557368301855007 1103 6 0.09045700966989045 1103 7 0.11739077928059061 1103 8 0.1259929711224217 1103 9 0.16661135240924096 1103 10-11 0.18704420911222996 1103 12-13 0.1966749383461277 1103 14-15 0.26152596935764194 1103 16-17 0.24941663978112416 1103 18-19 0.22209501614219107 1103 20-21 0.21096027119944694 1103 22-23 0.3040238479405062 1103 24-25 0.2935226689889774 1103 26-27 0.29388678778838795 1103 28-29 0.11008316355713532 1103 30-31 0.16224892016037273 1103 32-33 0.20610204207704186 1103 34-35 0.2082257805399763 1103 36-37 0.21660630359865252 1103 38-39 0.24612803328562904 1103 40-41 0.2601144063379408 1103 42-43 0.1852778226666132 1103 44-45 0.2436711777073839 1103 46-47 0.30963775983211406 1103 48-49 0.2450737759956354 1103 50-51 0.2827254197025866 1103 52-53 0.1623706673106895 1103 54-55 0.22315061879597664 1103 56-57 0.24422839448325107 1103 58-59 0.1567784199657254 1103 60-61 0.16572562883175834 1103 62-63 0.13934008872414694 1103 64-65 0.22514766164261601 1103 66-67 0.16632080736931698 1103 68-69 0.05049767338202571 1103 70-71 0.11028205536641167 1103 72-73 0.153388734567109 1103 74-75 0.1384567049868437 1103 76-77 0.1776738491200902 1103 78-79 0.3739660300703207 1103 80-81 0.3572383350124113 1103 82-83 0.24049839697920206 1103 84-85 0.14248335691270242 1103 86-87 0.14307933622090374 1103 88-89 0.4024598210404804 1103 90-91 0.4054544325245395 1103 92-93 0.2935629772279249 1103 94-95 0.29049388500127904 1103 96-97 0.11211830251864541 1103 98-99 0.13207084404585956 1103 100-101 0.08311306022641318 1104 1 0.14148576081232989 1104 2 0.07245759623969406 1104 3 0.10987678298575076 1104 4 0.09272920219109437 1104 5 0.11280830894882854 1104 6 0.10251914944942087 1104 7 0.16994659596684158 1104 8 0.15322727063933428 1104 9 0.12501976081765065 1104 10-11 0.15603960202066247 1104 12-13 0.20613614644863532 1104 14-15 0.22578510426895093 1104 16-17 0.1793131900581102 1104 18-19 0.09472688714362221 1104 20-21 0.21257846548068215 1104 22-23 0.1469764667083453 1104 24-25 0.08442506206528577 1104 26-27 0.16911912416202313 1104 28-29 0.24474360039148735 1104 30-31 0.29415291266219157 1104 32-33 0.2508416574688255 1104 34-35 0.27219965348124475 1104 36-37 0.3248082555506002 1104 38-39 0.3465960867428137 1104 40-41 0.4398745624567866 1104 42-43 0.44668993353524655 1104 44-45 0.42868460728603 1104 46-47 0.45742829104656124 1104 48-49 0.32882724996650126 1104 50-51 0.29023397639592474 1104 52-53 0.28862502726070005 1104 54-55 0.17452225440673885 1104 56-57 0.28542234464458716 1104 58-59 0.327228334165639 1104 60-61 0.30229453469414835 1104 62-63 0.25855697641929254 1104 64-65 0.21325572312024832 1104 66-67 0.22284534432863978 1104 68-69 0.26116299709082824 1104 70-71 0.34488630247065544 1104 72-73 0.2888907350147534 1104 74-75 0.28091154336559043 1104 76-77 0.37279326978298855 1104 78-79 0.4914796228556533 1104 80-81 0.4658921363292521 1104 82-83 0.539581408562217 1104 84-85 0.5363685853088036 1104 86-87 0.5025630435306994 1104 88-89 0.694478897570427 1104 90-91 0.7933893783399206 1104 92-93 0.5970889410799742 1104 94-95 0.43171464768943224 1104 96-97 0.526785337566114 1104 98-99 0.4868313498933219 1104 100-101 0.3587226371946848 1105 1 0.08034615432729453 1105 2 0.07254338376693426 1105 3 0.14256330021559194 1105 4 0.0976965147696447 1105 5 0.1353232963350095 1105 6 0.15583683230457268 1105 7 0.16929849889017845 1105 8 0.15031241617295876 1105 9 0.12047923836144037 1105 10-11 0.1201354308705973 1105 12-13 0.15879934619907488 1105 14-15 0.18159763309085264 1105 16-17 0.1805481800665163 1105 18-19 0.17108875001017054 1105 20-21 0.17917100524359597 1105 22-23 0.15709380008387797 1105 24-25 0.20873311049511045 1105 26-27 0.24459898048204565 1105 28-29 0.15588354595766418 1105 30-31 0.1533515622150574 1105 32-33 0.16089360469876368 1105 34-35 0.26425650834548264 1105 36-37 0.2715484990560739 1105 38-39 0.26979856113088374 1105 40-41 0.3831634121127365 1105 42-43 0.32481886122506154 1105 44-45 0.4393306246650681 1105 46-47 0.4451931699267746 1105 48-49 0.5551749844524068 1105 50-51 0.6467942356347578 1105 52-53 0.619047160668778 1105 54-55 0.5026294809038987 1105 56-57 0.47632135045869717 1105 58-59 0.4929648070888817 1105 60-61 0.391886608785633 1105 62-63 0.3581059985092949 1105 64-65 0.40351019220960893 1105 66-67 0.4587287185024138 1105 68-69 0.4341992652090667 1105 70-71 0.5363442835363657 1105 72-73 0.3056341658828998 1105 74-75 0.35329355443320054 1105 76-77 0.502892484923187 1105 78-79 0.6031016852827484 1105 80-81 0.626265023853783 1105 82-83 0.6704080657647502 1105 84-85 0.6302715109008368 1105 86-87 0.5280529027442107 1105 88-89 0.6970116641108071 1105 90-91 0.8106668619962107 1105 92-93 0.6895026788391441 1105 94-95 0.6832686000191472 1105 96-97 0.6721298100915014 1105 98-99 0.7519609240978369 1105 100-101 0.7841145584736076 1106 1 0.006391589065614767 1106 2 0.06517095823272001 1106 3 0.09794718715784967 1106 4 0.057555306000246276 1106 5 0.0510372810761055 1106 6 0.05428586075002784 1106 7 0.11971491056566919 1106 8 0.043530399713645807 1106 9 0.11279417147341775 1106 10-11 0.16750054144770843 1106 12-13 0.0888758042942257 1106 14-15 0.17592957729478087 1106 16-17 0.07463609608016952 1106 18-19 0.018680779105984868 1106 20-21 0.12270941618366038 1106 22-23 0.156485038187256 1106 24-25 0.19651234491528413 1106 26-27 0.23708773651623005 1106 28-29 0.2752802648264563 1106 30-31 0.373404075493859 1106 32-33 0.29218825178151775 1106 34-35 0.22453698918722864 1106 36-37 0.21734873442583336 1106 38-39 0.34751027505106435 1106 40-41 0.27626084280493757 1106 42-43 0.22445209922113918 1106 44-45 0.3159824331431764 1106 46-47 0.5092013891120288 1106 48-49 0.29916359021810024 1106 50-51 0.25439607689277466 1106 52-53 0.37313734270521337 1106 54-55 0.2760704943024379 1106 56-57 0.30856996260153124 1106 58-59 0.3113100935651971 1106 60-61 0.19107298883277224 1106 62-63 0.3095462642729885 1106 64-65 0.20764638170582117 1106 66-67 0.25685024231658815 1106 68-69 0.2341509459262383 1106 70-71 0.3041030648653873 1106 72-73 0.3573390154333751 1106 74-75 0.24200359206357547 1106 76-77 0.31804107625960754 1106 78-79 0.5420004745417586 1106 80-81 0.4827040835437444 1106 82-83 0.4896602520732607 1106 84-85 0.4491612753260661 1106 86-87 0.48608519018843666 1106 88-89 0.6262001967705402 1106 90-91 0.6614363937386329 1106 92-93 0.7538884230913219 1106 94-95 0.5613243502864265 1106 96-97 0.5712986660836812 1106 98-99 0.5000603220205981 1106 100-101 0.3921445777140846 1107 1 -0.0894817005807127 1107 2 0.02814926283443242 1107 3 0.014156565802736054 1107 4 0.0354696270618291 1107 5 0.05444684943757494 1107 6 0.04240483919537752 1107 7 0.06326860689505054 1107 8 0.06546377654798619 1107 9 0.022213041610420703 1107 10-11 0.09344195167150104 1107 12-13 0.10549365031149449 1107 14-15 0.12513359840345828 1107 16-17 0.008658522695121462 1107 18-19 0.09442400920979566 1107 20-21 0.10808185572816598 1107 22-23 0.1145659921168587 1107 24-25 0.0026698159216635986 1107 26-27 0.026074472595730924 1107 28-29 0.07999440533249214 1107 30-31 0.16903869951899964 1107 32-33 0.19386240897728158 1107 34-35 0.19642907893032913 1107 36-37 0.12455193687166144 1107 38-39 0.11381076429164949 1107 40-41 0.04901512069308467 1107 42-43 -0.05469907524357609 1107 44-45 0.08774893350941682 1107 46-47 0.039509559875270384 1107 48-49 0.053032667456811566 1107 50-51 -0.08029628118519838 1107 52-53 -0.07389515021476711 1107 54-55 -0.10804321988262444 1107 56-57 0.04562464811381517 1107 58-59 0.05181296950362935 1107 60-61 -0.09183743462745753 1107 62-63 0.02133956897672107 1107 64-65 -0.020814321852860473 1107 66-67 -0.06993349327613885 1107 68-69 0.013210093692379132 1107 70-71 -0.060065163687298195 1107 72-73 -0.06304721978769834 1107 74-75 -0.10223341291294474 1107 76-77 0.008622747456126234 1107 78-79 0.2695846342068329 1107 80-81 0.2235175495898467 1107 82-83 0.2518138142352164 1107 84-85 0.17041818305410317 1107 86-87 0.25682081455627426 1107 88-89 0.4707324367212067 1107 90-91 0.5134121778081884 1107 92-93 0.4382646944443245 1107 94-95 0.4670124434890681 1107 96-97 0.4623828821723599 1107 98-99 0.7676027552814624 1107 100-101 0.6182837932884979 1108 1 0.054567156161169805 1108 2 0.06548870874755153 1108 3 0.07455308719693932 1108 4 0.020101923749855644 1108 5 0.06126839694380237 1108 6 0.10247986597990177 1108 7 0.019270920291170057 1108 8 0.05138094757207767 1108 9 0.12404553077365676 1108 10-11 0.16248209102129607 1108 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-0.033220134938439116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 17.0 5 59.0 6 126.0 7 370.0 8 772.0 9 1566.0 10 2622.0 11 3767.0 12 5640.0 13 8097.0 14 9949.0 15 12978.0 16 17007.0 17 23056.0 18 22910.0 19 16484.0 20 15621.0 21 16908.0 22 17880.0 23 19720.0 24 22830.0 25 27458.0 26 33034.0 27 40489.0 28 49833.0 29 60675.0 30 73842.0 31 90915.0 32 112731.0 33 144682.0 34 196474.0 35 280281.0 36 445774.0 37 739415.0 38 638227.0 39 55754.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.214411412603496 19.325689923013687 15.203312005551266 23.256586658831544 2 15.52936739007101 20.399628426611137 39.6604405264372 24.410563656880655 3 19.21567466131335 23.992138354977275 30.107575483637678 26.684611500071696 4 13.311086727473365 17.051976633270783 36.567559648127485 33.06937699112837 5 14.20192769281604 36.994245600034915 34.04460127556905 14.759225431579997 6 32.30512659056478 35.943771469896944 17.222208367882594 14.528893571655685 7 28.720347383711868 32.39945386193181 20.513750085723725 18.3664486686326 8 26.147357527166626 35.65374472409429 19.453519036901724 18.745378711837354 9 26.037007712018156 17.157338886152658 20.208822997649612 36.59683040417958 10-11 25.243330694081635 26.558332657934276 26.92445090056671 21.273885747417378 12-13 25.784294166422484 23.780166958646877 27.651621892904572 22.783916982026074 14-15 22.731754547900206 25.374987258055015 25.840508568042296 26.052749626002488 16-17 22.45096954290194 28.493074248236027 26.520181860406396 22.535774348455636 18-19 22.342954133130505 27.17135081889538 28.57996309203923 21.905731955934886 20-21 23.09420569953678 26.29308786214378 28.548401174570913 22.064305263748526 22-23 22.759898752485988 26.17580221821832 28.470579617079906 22.593719412215787 24-25 22.684124605631524 26.453852381111208 28.538998253416402 22.32302475984086 26-27 22.49031819838922 26.60537417725923 28.56667253229982 22.337635092051737 28-29 22.307963266603906 26.750384977462456 28.70900067955536 22.232651076378282 30-31 22.552182370214275 26.459391891470645 28.447714761313975 22.5407109770011 32-33 22.390694455701095 26.56286822237046 28.46674542858746 22.579691893340982 34-35 22.879926979652872 26.527811418968273 28.221425848217805 22.37083575316105 36-37 22.940948955416957 26.34339111482003 28.30768520846841 22.4079747212946 38-39 22.569711483756407 26.611601190489182 28.388574945047274 22.430112380707143 40-41 22.65838580967903 26.465101378921695 28.39747801761397 22.479034793785306 42-43 22.57555376479005 26.504832728820084 28.059037070404617 22.86057643598524 44-45 22.725750944525494 26.610696517412936 27.94166697839125 22.721885559670323 46-47 22.78565138444034 26.883812997295802 27.635642422400426 22.694893195863433 48-49 22.8770441212227 26.78266385700659 27.641522079314708 22.698769942456 50-51 22.71661606369117 26.96863758502235 27.599049869388214 22.715696481898267 52-53 22.903680820952747 26.99033348088205 27.328801925199038 22.77718377296617 54-55 22.657903104134068 26.84530452184864 27.374391361542155 23.12240101247514 56-57 22.711160917462077 27.096911452066408 27.35787006153405 22.834057568937464 58-59 22.863362195334645 26.859102175786436 27.470642227716528 22.806893401162387 60-61 22.82588094466196 26.92009152347939 27.67381979600489 22.58020773585376 62-63 22.57091578168873 26.958619114899975 27.701151127241214 22.769313976170082 64-65 22.48891318941196 26.76246115739242 27.62527866068928 23.12334699250634 66-67 22.56024040050125 26.928020124813745 27.384974345226592 23.126765129458413 68-69 22.447864063142934 26.859405977630647 27.527494108405342 23.165235850821084 70-71 22.61870750732665 26.716115237020688 27.522775628709596 23.142401626943066 72-73 22.64432758308967 26.664661251005306 27.501885921982055 23.189125243922966 74-75 22.477976795366555 27.088993696968185 27.13166852661176 23.3013609810535 76-77 22.573481958189927 26.696795325350305 27.308575403847495 23.421147312612277 78-79 22.538778296622798 26.839412963921223 27.28558158093255 23.33622715852343 80-81 22.457423241664475 27.055402974150244 27.25348950409476 23.233684280090518 82-83 22.570277869562776 26.884846538943506 27.28162270338344 23.263252888110276 84-85 22.50777747990948 26.83236803221965 27.162716101721333 23.49713838614954 86-87 22.39374552598781 26.791898441561525 27.24048544943578 23.573870583014887 88-89 22.33283481021004 26.707496445656837 27.26937126801789 23.690297476115234 90-91 22.31670219833318 26.749017541208676 27.347276012456966 23.58700424800118 92-93 22.317018809344198 26.56699854737249 27.310534978397623 23.80544766488569 94-95 22.156840129925996 26.419912218904106 27.51879687529224 23.904450775877656 96-97 22.081902006870365 26.434142357495993 27.58248181098386 23.90147382464978 98-99 22.05121291279871 26.5146447297053 27.216612946464753 24.21752941103124 100-101 21.98105751185289 26.050807464587844 27.759939413464608 24.20819561009466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 187.0 2 157.0 3 210.5 4 196.5 5 197.0 6 372.0 7 895.5 8 1534.5 9 1927.0 10 1950.5 11 1952.5 12 2194.5 13 2472.0 14 2787.5 15 3100.0 16 3285.0 17 3491.5 18 3926.5 19 4222.0 20 4612.0 21 5459.0 22 6496.5 23 8107.0 24 10097.0 25 12541.5 26 16075.5 27 20281.5 28 24109.5 29 28514.0 30 33689.0 31 39156.0 32 45292.5 33 50809.0 34 56860.5 35 62526.5 36 68498.5 37 75791.0 38 81623.0 39 85746.5 40 89877.0 41 96533.0 42 103020.5 43 107576.5 44 111557.5 45 116680.5 46 118979.0 47 123493.0 48 135856.5 49 138058.5 50 128925.5 51 123377.5 52 120881.5 53 118982.5 54 116323.0 55 108657.5 56 95751.5 57 85738.5 58 77582.0 59 65624.5 60 54388.0 61 45418.0 62 37274.5 63 28655.0 64 21293.0 65 15807.5 66 11939.0 67 10070.0 68 7849.0 69 5202.5 70 3668.5 71 2892.5 72 2341.0 73 1846.5 74 1465.0 75 1184.0 76 796.0 77 404.0 78 223.0 79 140.5 80 72.0 81 37.0 82 25.5 83 16.5 84 9.0 85 4.5 86 2.5 87 1.0 88 0.5 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2715104208879108 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002213230755802717 16-17 7.793066041558862E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.351679249870635E-5 26-27 8.884095287377104E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003335432265787193 36-37 0.021680309727616757 38-39 0.006951414909070506 40-41 0.018157843876832152 42-43 0.00514342358742885 44-45 6.23445283324709E-4 46-47 1.5586132083117725E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.2082556624417855E-4 60-61 0.001870335849974127 62-63 0.0011689599062338293 64-65 4.6758396249353176E-5 66-67 0.0 68-69 6.23445283324709E-4 70-71 1.5586132083117724E-5 72-73 0.0 74-75 0.0 76-77 0.233277638888023 78-79 0.0 80-81 0.001200132170400065 82-83 0.0 84-85 0.0 86-87 0.10480115212688358 88-89 0.35968117008210776 90-91 0.35958765328960907 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.0261971708053042E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3207980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.296904165752224 #Duplication Level Percentage of deduplicated Percentage of total 1 70.80264767907263 34.90351337316763 2 15.220983094843552 15.006946898700747 3 5.739532209559179 8.488235078726609 4 2.7311594002899007 5.3855081286993824 5 1.5416628980949034 3.7999604071640145 6 0.9267403016547062 2.7411256702327416 7 0.5929074933274315 2.0459952714403142 8 0.40969174405962405 1.6157227715525735 9 0.3038995287640588 1.3483175350949161 >10 1.569640720122781 13.786748506604162 >50 0.10055297243273376 3.3902135434852885 >100 0.056909289852053964 5.214116065499433 >500 0.002342908850219401 0.7722144080964973 >1k 0.0012031153546590964 1.0328032133406893 >5k 1.266437215430628E-4 0.46857912819491887 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7808 0.2433930386099664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7203 0.22453381878939394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4690 0.14619791893964426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4477 0.1395582266722361 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.117226416623545E-5 0.0 5 0.0 0.0 0.0 3.117226416623545E-5 0.0 6 0.0 0.0 0.0 3.117226416623545E-5 0.0 7 0.0 0.0 0.0 3.117226416623545E-5 0.0 8 0.0 0.0 0.0 3.117226416623545E-5 0.0 9 0.0 0.0 0.0 3.117226416623545E-5 0.0 10-11 0.0 0.0 0.0 3.117226416623545E-5 0.0 12-13 0.0 0.0 0.0 3.117226416623545E-5 0.0 14-15 0.0 0.0 0.0 3.117226416623545E-5 0.0 16-17 0.0 0.0 0.0 3.117226416623545E-5 0.0 18-19 0.0 0.0 0.0 3.117226416623545E-5 0.0 20-21 0.0 0.0 0.0 3.117226416623545E-5 0.0 22-23 0.0 0.0 0.0 1.4027518874805953E-4 0.0 24-25 0.0 0.0 0.0 3.740671699948254E-4 0.0 26-27 0.0 0.0 0.0 5.766868870753559E-4 0.0 28-29 0.0 0.0 0.0 0.0016833022649767144 0.0 30-31 0.0 0.0 0.0 0.005299284908260027 0.0 32-33 0.0 0.0 0.0 0.013076764817735773 0.0 34-35 0.0 0.0 0.0 0.024205263125081828 0.0 36-37 0.0 0.0 0.0 0.0444984070973011 0.0 38-39 0.0 0.0 0.0 0.07400295513064295 0.0 40-41 0.0 0.0 0.0 0.10737286392059801 0.0 42-43 0.0 0.0 0.0 0.14387558525925973 0.0 44-45 0.0 0.0 0.0 0.18962088292321025 0.0 46-47 0.0 0.0 0.0 0.24878584031072512 0.0 48-49 0.0 0.0 0.0 0.3144502147769001 0.0 50-51 0.0 0.0 0.0 0.38455663688676367 0.0 52-53 0.0 0.0 0.0 0.45781145767741693 0.0 54-55 0.0 0.0 0.0 0.5398568569629487 0.0 56-57 0.0 0.0 0.0 0.6412758184277957 0.0 58-59 0.0 0.0 0.0 0.7538700365962381 0.0 60-61 0.0 0.0 0.0 0.8788084713745099 0.0 62-63 0.0 0.0 0.0 1.0063342040785792 0.0 64-65 0.0 0.0 0.0 1.147341941034545 0.0 66-67 0.0 1.5586132083117724E-5 0.0 1.315485133947219 0.0 68-69 0.0 3.117226416623545E-5 0.0 1.504124090549193 3.117226416623545E-5 70-71 0.0 3.117226416623545E-5 0.0 1.714910940841277 3.117226416623545E-5 72-73 0.0 3.117226416623545E-5 0.0 1.9309035592491224 3.117226416623545E-5 74-75 0.0 3.117226416623545E-5 0.0 2.1600664592672025 3.117226416623545E-5 76-77 0.0 3.117226416623545E-5 0.0 2.416972674393232 3.117226416623545E-5 78-79 0.0 3.117226416623545E-5 0.0 2.7095399597254346 3.117226416623545E-5 80-81 0.0 3.117226416623545E-5 0.0 3.030193455071416 3.117226416623545E-5 82-83 0.0 3.117226416623545E-5 0.0 3.363580820329304 3.117226416623545E-5 84-85 0.0 3.117226416623545E-5 0.0 3.704402770590839 3.117226416623545E-5 86-87 0.0 3.117226416623545E-5 0.0 4.075477403225706 3.117226416623545E-5 88-89 0.0 3.117226416623545E-5 0.0 4.475261691157676 3.117226416623545E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12635 0.0 25.723427 1 GTATCAA 16885 0.0 24.18074 1 GTACATG 11405 0.0 23.490728 1 TACATGG 11645 0.0 22.5349 2 TAGATCG 130 2.9625717E-4 21.901707 5 ACATGGG 11760 0.0 21.467146 3 GAGTACT 9545 0.0 21.155376 12-13 ATCAACG 19440 0.0 20.699966 3 CAACGCA 19515 0.0 20.644728 5 TCAACGC 19515 0.0 20.62041 4 GTACTTT 9930 0.0 20.287361 14-15 AACGCAG 19955 0.0 20.18952 6 TATCAAC 20175 0.0 20.016401 2 AGTACTT 10165 0.0 19.048021 12-13 AGAGTAC 16680 0.0 18.96245 10-11 TGCGCCG 180 1.2434556E-4 18.454214 5 ACGCAGA 22110 0.0 18.11439 7 CGCAGAG 22110 0.0 18.007076 8 CATGGGG 7235 0.0 17.70905 4 TACTTTT 11300 0.0 17.071796 14-15 >>END_MODULE