FastQCFastQC Report
Fri 27 May 2016
SRR939321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520038
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.28344810673489845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.21944113541145013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.14225975957505405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.046.9074131
GTATCAA80500.036.7217181
ATCAACG100050.029.1441763
TATCAAC102350.028.8135192
TCAACGC101500.028.7278334
CAACGCA102800.028.4106645
AACGCAG107950.027.1280026
GTACATG95850.024.900421
TACATGG93350.024.5824322
ACGCAGA122600.023.7317567
CGCAGAG122450.023.6456748
ACATGGG94000.023.4036663
GCAGAGT136650.020.9098559
GAGTACT77950.020.48457312-13
TGGTATC26100.019.8006652
GTGGTAT28600.019.4111231
GTGTAGC15400.019.4111211
CAGAGTA130750.019.1698410-11
GTATAGG7050.018.8451561
CATGGGA47150.018.804154
Produced by FastQC (version 0.11.5)