FastQCFastQC Report
Fri 27 May 2016
SRR939321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520038
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71500.28372588032402685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61500.24404393901996715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40320.1599975873379687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.046.776111
GTATCAA82050.037.9429361
ATCAACG105550.028.9548723
TATCAAC106200.028.8670252
TCAACGC107200.028.464944
CAACGCA108500.028.1676245
AACGCAG113150.027.051996
GTACCGT2950.024.1303316
GTACATG95850.023.7392771
TACATGG95550.023.3929122
ACGCAGA131450.023.17767
CGCAGAG133050.022.8275418
ACATGGG97500.022.0002633
GTGTAGC16600.020.6469611
TATACCG1150.003744397320.6331835
GCAGAGT148050.020.3227989
GAGTACT85100.020.07672312-13
GGACCGA4800.019.7734666
GTACTTT88550.019.0801314-15
AGTACTT90000.019.0363912-13
Produced by FastQC (version 0.11.5)