##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939321_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2520038 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32757640956208 34.0 31.0 34.0 31.0 34.0 2 32.53852560953446 34.0 31.0 34.0 31.0 34.0 3 32.62937662051128 34.0 31.0 34.0 31.0 34.0 4 36.148782677086615 37.0 35.0 37.0 35.0 37.0 5 36.09124386219573 37.0 35.0 37.0 35.0 37.0 6 36.1150784234206 37.0 36.0 37.0 35.0 37.0 7 36.06363277061695 37.0 35.0 37.0 35.0 37.0 8 36.03293918583768 37.0 35.0 37.0 35.0 37.0 9 37.74805498964698 39.0 38.0 39.0 35.0 39.0 10-11 37.67222974415465 39.0 37.0 39.0 35.0 39.0 12-13 37.5977185661486 39.0 37.0 39.0 35.0 39.0 14-15 38.909805526742055 40.0 38.0 41.0 35.0 41.0 16-17 38.90608336064774 40.0 38.0 41.0 35.0 41.0 18-19 39.00641637149916 40.0 38.5 41.0 35.0 41.0 20-21 38.98627282604468 40.0 38.5 41.0 35.0 41.0 22-23 38.90935731921503 40.0 38.0 41.0 35.0 41.0 24-25 38.84917945681771 40.0 38.0 41.0 35.0 41.0 26-27 38.69675854094264 40.0 38.0 41.0 34.0 41.0 28-29 38.535240738433316 40.0 38.0 41.0 34.0 41.0 30-31 38.341236322626884 40.0 38.0 41.0 34.0 41.0 32-33 38.28901707037751 40.0 38.0 41.0 34.0 41.0 34-35 38.20192989153338 40.0 38.0 41.0 34.0 41.0 36-37 38.05697493450496 40.0 38.0 41.0 33.0 41.0 38-39 37.92630170656157 40.0 37.5 41.0 33.0 41.0 40-41 37.74542149761234 40.0 37.0 41.0 33.0 41.0 42-43 37.5640418914318 40.0 37.0 41.0 33.0 41.0 44-45 37.328762502787654 40.0 37.0 41.0 32.0 41.0 46-47 37.03490304511281 40.0 36.5 41.0 31.0 41.0 48-49 36.73085048717519 40.0 36.0 41.0 30.5 41.0 50-51 36.48909242638405 39.0 36.0 41.0 30.0 41.0 52-53 36.22722633547589 39.0 35.0 41.0 30.0 41.0 54-55 35.978571354876394 39.0 35.0 40.0 29.0 41.0 56-57 35.666861967954446 38.5 35.0 40.0 28.5 41.0 58-59 35.352839520673896 38.0 34.5 40.0 28.0 41.0 60-61 35.019569744583215 38.0 34.0 40.0 27.5 41.0 62-63 34.66530623744563 37.0 34.0 40.0 27.0 41.0 64-65 34.359104108747566 37.0 34.0 40.0 26.0 41.0 66-67 33.94772043119984 36.5 33.5 39.0 26.0 41.0 68-69 33.48057013425988 36.0 33.0 39.0 25.5 40.0 70-71 33.0034598684623 35.0 32.0 38.5 24.5 40.0 72-73 32.51033833616795 35.0 32.0 37.5 22.5 40.0 74-75 31.98758570307273 35.0 31.0 37.0 22.0 39.0 76-77 30.036329412492986 33.5 29.5 35.0 19.0 37.5 78-79 30.996784572296132 34.0 30.5 36.0 20.0 38.0 80-81 31.005432854583937 34.0 31.0 36.0 20.0 37.5 82-83 30.64670770837583 34.0 31.0 35.0 18.0 37.0 84-85 30.192881813686938 34.0 30.0 35.0 15.0 36.5 86-87 29.574475265849166 34.0 29.5 35.0 7.5 36.0 88-89 28.982287965498934 34.0 29.0 35.0 2.0 35.5 90-91 28.494943925448744 33.5 29.0 35.0 2.0 35.0 92-93 28.120822781243774 33.0 28.5 35.0 2.0 35.0 94-95 27.59411088245495 33.0 27.0 35.0 2.0 35.0 96-97 26.863882211299988 33.0 25.5 34.5 2.0 35.0 98-99 25.85812158388088 32.0 24.5 34.0 2.0 35.0 100-101 21.521688958658558 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2910132335821842 1101 2 0.2746770677930783 1101 3 0.27463528139625026 1101 4 0.14449782058947136 1101 5 0.16576611168843414 1101 6 0.17525290104887148 1101 7 0.19162010626369863 1101 8 0.19085205038493314 1101 9 0.27805079462570603 1101 10-11 0.30641663551266163 1101 12-13 0.30970425082211506 1101 14-15 0.25958969157757394 1101 16-17 0.2669232658379812 1101 18-19 0.3055793793098758 1101 20-21 0.3129612996428648 1101 22-23 0.329004363743465 1101 24-25 0.2984307686466181 1101 26-27 0.25125377308300756 1101 28-29 0.28162680160743037 1101 30-31 0.34089967183201964 1101 32-33 0.4790641589117115 1101 34-35 0.43248365527441024 1101 36-37 0.3649826067836628 1101 38-39 0.5062937402259493 1101 40-41 0.47268042849680114 1101 42-43 0.44497872202207134 1101 44-45 0.6321543331721742 1101 46-47 0.6288860271578827 1101 48-49 0.45595840536498145 1101 50-51 0.6029106966572826 1101 52-53 0.673291155100344 1101 54-55 0.6110474931411289 1101 56-57 0.5462059292269856 1101 58-59 0.5873096044747754 1101 60-61 0.503078138102822 1101 62-63 0.4879565750484147 1101 64-65 0.5906310699008444 1101 66-67 0.5572172630662564 1101 68-69 0.5190220014526759 1101 70-71 0.44612614841582143 1101 72-73 0.5276949414466614 1101 74-75 0.5290619024050542 1101 76-77 0.5556560234905916 1101 78-79 0.7084033195340602 1101 80-81 0.775361578949795 1101 82-83 0.8208471938530124 1101 84-85 0.7642109649726798 1101 86-87 0.6827089349906466 1101 88-89 0.8984167206492231 1101 90-91 1.205202971043395 1101 92-93 1.0375072772343223 1101 94-95 0.7110807144170757 1101 96-97 0.5368222654756387 1101 98-99 0.22910873557076172 1101 100-101 0.39729450416073675 1102 1 0.2439302906445846 1102 2 0.22488220935117198 1102 3 0.2096022817177854 1102 4 0.20450194542755895 1102 5 0.2179365496866481 1102 6 0.2013202029042489 1102 7 0.24644072352098334 1102 8 0.24661954973787203 1102 9 0.27526156275119007 1102 10-11 0.24866383873415998 1102 12-13 0.26405779540765906 1102 14-15 0.3165957134906279 1102 16-17 0.2930623336440519 1102 18-19 0.3350941733201722 1102 20-21 0.26758243287493855 1102 22-23 0.3516685768001153 1102 24-25 0.37046752138244443 1102 26-27 0.35533504524695303 1102 28-29 0.3349084920198351 1102 30-31 0.2612272945951233 1102 32-33 0.40091395988535083 1102 34-35 0.36813423357943265 1102 36-37 0.48673544095445465 1102 38-39 0.4519380923235303 1102 40-41 0.5044168509534472 1102 42-43 0.569596351673546 1102 44-45 0.5605555766998478 1102 46-47 0.6658321512804619 1102 48-49 0.5772287425403064 1102 50-51 0.5205909640589752 1102 52-53 0.5800530656676983 1102 54-55 0.40711748451558094 1102 56-57 0.35318483198499706 1102 58-59 0.4177102064908027 1102 60-61 0.5038386429533972 1102 62-63 0.5150162334892343 1102 64-65 0.34168462716471737 1102 66-67 0.2596464187773435 1102 68-69 0.357647935700264 1102 70-71 0.24214277786222027 1102 72-73 0.4301351200961676 1102 74-75 0.4529174497182922 1102 76-77 0.4083442864176625 1102 78-79 0.5178266465256307 1102 80-81 0.5632269791340683 1102 82-83 0.5006293720207005 1102 84-85 0.4598924659307606 1102 86-87 0.3066441551679979 1102 88-89 0.3435080756857971 1102 90-91 0.5520544322741046 1102 92-93 0.30516067865799457 1102 94-95 0.2542514879722262 1102 96-97 0.07287788807982665 1102 98-99 -0.10816679134419971 1102 100-101 -0.2760481162254287 1103 1 0.16388382145265012 1103 2 0.17681617052117815 1103 3 0.24053309597245942 1103 4 0.1386892758072804 1103 5 0.19751832695926907 1103 6 0.1786515877789583 1103 7 0.20979230432402574 1103 8 0.23552454691569835 1103 9 0.28553697583215154 1103 10-11 0.23838310991784795 1103 12-13 0.21001706334391912 1103 14-15 0.3287704871369499 1103 16-17 0.2994351271306286 1103 18-19 0.3596345071870104 1103 20-21 0.3554759905902998 1103 22-23 0.33735426800034674 1103 24-25 0.27695223526057333 1103 26-27 0.27930398588855354 1103 28-29 0.3397579890546183 1103 30-31 0.36782199587013054 1103 32-33 0.4342551218471087 1103 34-35 0.3614931404322803 1103 36-37 0.2924951851806057 1103 38-39 0.3328171023209805 1103 40-41 0.4649076962906733 1103 42-43 0.4613439141672586 1103 44-45 0.5670754710810186 1103 46-47 0.5456347731944291 1103 48-49 0.424910212080178 1103 50-51 0.3098318190611735 1103 52-53 0.15476243991866312 1103 54-55 0.20489309089601448 1103 56-57 0.36302590116301303 1103 58-59 0.2156021791505296 1103 60-61 0.3805880031321678 1103 62-63 0.3589560052412253 1103 64-65 0.31953376466249495 1103 66-67 0.22562368289400325 1103 68-69 0.2497470132752042 1103 70-71 0.2520288858161095 1103 72-73 0.2636470621372524 1103 74-75 0.21578558855370744 1103 76-77 0.4858639449734845 1103 78-79 0.538219547022802 1103 80-81 0.4967674328465783 1103 82-83 0.41878125514411124 1103 84-85 0.32451962991829575 1103 86-87 0.2550446987261026 1103 88-89 0.3546308169815262 1103 90-91 0.4454516124214756 1103 92-93 0.36124226649713975 1103 94-95 0.10590719455910147 1103 96-97 0.08216389783758515 1103 98-99 -0.026682850663934943 1103 100-101 -0.1838345087161315 1104 1 0.06419435230174031 1104 2 0.09680687726218906 1104 3 0.08399268162910545 1104 4 0.0716304789443214 1104 5 0.07748795386891771 1104 6 0.10452764743714482 1104 7 0.14086578906948688 1104 8 0.1123027326902104 1104 9 0.14573689423280456 1104 10-11 0.043107372123756704 1104 12-13 0.06883885943621237 1104 14-15 0.15780625485081146 1104 16-17 0.1156716972380778 1104 18-19 0.11566945890739078 1104 20-21 0.13905128865457783 1104 22-23 0.2064864816093106 1104 24-25 0.1484258968046177 1104 26-27 0.21984866153114524 1104 28-29 0.14330302736097877 1104 30-31 0.22502823822019025 1104 32-33 0.1739719041128538 1104 34-35 0.2135257575046836 1104 36-37 0.3040562260256152 1104 38-39 0.27508896414608586 1104 40-41 0.2833383821785205 1104 42-43 0.18964472109414032 1104 44-45 0.1202754121481604 1104 46-47 0.07849947694347037 1104 48-49 0.1845746347647932 1104 50-51 0.2632448250904602 1104 52-53 0.19734539925498495 1104 54-55 0.0800171899032236 1104 56-57 -0.02115175874904196 1104 58-59 -0.04543809919391606 1104 60-61 0.11819510227537222 1104 62-63 0.14888131917204817 1104 64-65 0.13149316898726227 1104 66-67 0.11886623695356491 1104 68-69 0.06378890094010359 1104 70-71 0.006673248661201114 1104 72-73 0.13403783234442557 1104 74-75 0.29018492669232643 1104 76-77 0.2221597879400754 1104 78-79 0.36238768701101165 1104 80-81 0.4734543860176217 1104 82-83 0.34421928497320664 1104 84-85 0.3979375092419275 1104 86-87 0.1266747590189503 1104 88-89 0.31618335789453056 1104 90-91 0.21567181732650553 1104 92-93 0.24993395696361276 1104 94-95 0.23256468129920904 1104 96-97 0.17293353834712732 1104 98-99 0.12266945451762368 1104 100-101 0.06948443139901528 1105 1 0.10445536465577732 1105 2 0.13789232764996484 1105 3 0.13593102426512615 1105 4 0.11963358043579575 1105 5 0.12274537587052947 1105 6 0.08965570930939037 1105 7 0.1474824235930967 1105 8 0.07000497876455114 1105 9 0.13760138842287972 1105 10-11 0.11765463853149072 1105 12-13 0.15549796935113847 1105 14-15 0.22828173927978668 1105 16-17 0.25354525953024165 1105 18-19 0.22842120886845407 1105 20-21 0.21982338454295558 1105 22-23 0.2224460905921788 1105 24-25 0.2234548613684595 1105 26-27 0.2156181340448029 1105 28-29 0.2299465581905551 1105 30-31 0.24975063503561046 1105 32-33 0.14948426799730896 1105 34-35 0.3028055560868026 1105 36-37 0.2259333804609085 1105 38-39 0.2553387130004907 1105 40-41 0.3284955250100907 1105 42-43 0.28209244610000894 1105 44-45 0.17643873110217356 1105 46-47 0.20802444018936228 1105 48-49 0.3847913989350431 1105 50-51 0.4305438772732302 1105 52-53 0.5262969805321518 1105 54-55 0.5243641307617963 1105 56-57 0.49852668534800415 1105 58-59 0.5761809259134836 1105 60-61 0.4257415574962451 1105 62-63 0.48127381900640387 1105 64-65 0.5013693958402285 1105 66-67 0.5634729187695555 1105 68-69 0.7253698020558872 1105 70-71 0.595742640056173 1105 72-73 0.4544739213851656 1105 74-75 0.47004663097263943 1105 76-77 0.5484802049581674 1105 78-79 0.5697045745604576 1105 80-81 0.5980681769104841 1105 82-83 0.654004383130399 1105 84-85 0.631791577979719 1105 86-87 0.6573922385736424 1105 88-89 0.7317877816384879 1105 90-91 0.887382257641594 1105 92-93 0.8885391990572273 1105 94-95 0.9262946366570688 1105 96-97 0.6858208442576874 1105 98-99 0.5950477361982287 1105 100-101 0.6764597430254895 1106 1 0.0937157719667212 1106 2 0.07889937190611107 1106 3 0.10290299484720578 1106 4 0.08154506148085261 1106 5 0.021641661770424037 1106 6 0.03684822256688847 1106 7 0.1652285785233687 1106 8 0.04079087157210637 1106 9 0.17030801896343917 1106 10-11 0.171207914106418 1106 12-13 0.15704241854901824 1106 14-15 0.16183147896671102 1106 16-17 0.07192362122990659 1106 18-19 0.15200841729320302 1106 20-21 0.18347412220091286 1106 22-23 0.3260476093561451 1106 24-25 0.17681157826308436 1106 26-27 0.22503994130055105 1106 28-29 0.2077610291458214 1106 30-31 0.2677198678062851 1106 32-33 0.19170994271396324 1106 34-35 0.21159632408335227 1106 36-37 0.14290422522957869 1106 38-39 0.24893433583797986 1106 40-41 0.2684610667806808 1106 42-43 0.24329416434143525 1106 44-45 0.42121416468626904 1106 46-47 0.3481716131885193 1106 48-49 0.22780964628542932 1106 50-51 0.2519146509905781 1106 52-53 0.3317239848761204 1106 54-55 0.25244650360950516 1106 56-57 0.42279905546875085 1106 58-59 0.3178994008449578 1106 60-61 0.33018635219374914 1106 62-63 0.4573792218266135 1106 64-65 0.4692458532473722 1106 66-67 0.5416873453594278 1106 68-69 0.6004310337958216 1106 70-71 0.4568360300304306 1106 72-73 0.564027073537595 1106 74-75 0.5429337085528587 1106 76-77 0.6742830975480629 1106 78-79 0.7142698563098584 1106 80-81 0.7054535962092565 1106 82-83 0.7258428697703252 1106 84-85 0.6079938841459231 1106 86-87 0.6858559702864717 1106 88-89 1.0589880171419708 1106 90-91 1.0416364563001252 1106 92-93 1.0502282694105567 1106 94-95 1.2660701244469976 1106 96-97 1.0616482962584257 1106 98-99 0.9012588070302385 1106 100-101 0.9859886276446268 1107 1 -0.012724948379457146 1107 2 -0.005261507731844972 1107 3 -0.0063351957213058085 1107 4 -0.046365579345746255 1107 5 0.002897907836533875 1107 6 0.03137686450205024 1107 7 -0.002119549843072832 1107 8 -0.01480968843353736 1107 9 -0.014687129668750742 1107 10-11 0.01778174850315395 1107 12-13 -0.002748559361712921 1107 14-15 -0.004773316573199793 1107 16-17 1.6014863217606035E-4 1107 18-19 0.030263226254504616 1107 20-21 0.016010085392871076 1107 22-23 0.04978119772002998 1107 24-25 0.08041844112997154 1107 26-27 0.050806052134277024 1107 28-29 0.08762216923404509 1107 30-31 0.10800147256106385 1107 32-33 0.04772304084747958 1107 34-35 0.06924636915974958 1107 36-37 -0.02658996791736712 1107 38-39 0.07139873475998826 1107 40-41 0.2024164367548309 1107 42-43 0.18453769724992242 1107 44-45 0.12945974733562338 1107 46-47 0.01591710766752641 1107 48-49 0.023976817913215598 1107 50-51 0.21386230929632433 1107 52-53 0.1984620511428119 1107 54-55 0.206741087232011 1107 56-57 0.2341056281808349 1107 58-59 0.24208744622414713 1107 60-61 0.17774892556579402 1107 62-63 0.03631566834877731 1107 64-65 0.1381645010291166 1107 66-67 0.32476685519301896 1107 68-69 0.22828148216964195 1107 70-71 0.09404604509533243 1107 72-73 0.18497169696065896 1107 74-75 0.11409885378133566 1107 76-77 0.06730863881250926 1107 78-79 0.20196357728578462 1107 80-81 0.22248406350253447 1107 82-83 0.31764123640455466 1107 84-85 0.26204051962762165 1107 86-87 0.33478928488260706 1107 88-89 0.5398689596678494 1107 90-91 0.6899556959663293 1107 92-93 0.6566895335288052 1107 94-95 0.8504326048364881 1107 96-97 0.6210066079408598 1107 98-99 0.8054718310782825 1107 100-101 0.7931133037204994 1108 1 0.0892803126807209 1108 2 0.06352143367907104 1108 3 0.06318923632333906 1108 4 0.06325769685991389 1108 5 0.1036867480118957 1108 6 0.05037088602456663 1108 7 0.10095019906086833 1108 8 0.10732805568763126 1108 9 0.20885627407776042 1108 10-11 0.0771673204441683 1108 12-13 0.05506969492286373 1108 14-15 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-0.41292201320776556 2316 84-85 -0.36549698401472597 2316 86-87 -0.6117485570377426 2316 88-89 -1.0499991119131096 2316 90-91 -1.098263019180152 2316 92-93 -0.8365809113570002 2316 94-95 -0.6957724854100533 2316 96-97 -0.646770570686666 2316 98-99 -0.23041829220897725 2316 100-101 0.22703103183207318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 19.0 5 35.0 6 105.0 7 253.0 8 602.0 9 1245.0 10 2100.0 11 3315.0 12 4799.0 13 6628.0 14 8571.0 15 11490.0 16 14517.0 17 19802.0 18 19097.0 19 14011.0 20 13476.0 21 14036.0 22 15049.0 23 16689.0 24 19631.0 25 22989.0 26 27642.0 27 33181.0 28 40118.0 29 49335.0 30 60103.0 31 73698.0 32 90988.0 33 117393.0 34 160151.0 35 231213.0 36 366587.0 37 581296.0 38 448544.0 39 31321.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51017060184651 19.29270239915563 14.914189651178802 23.28293734781906 2 15.608772566127971 20.275130771837567 40.1941558024125 23.921940859621955 3 19.38125536202232 24.0904700643403 29.757765557503497 26.770509016133882 4 13.089802614087564 16.85303951765807 36.72242243966162 33.334735428592744 5 13.8193154230214 37.31150085832039 34.26801500612292 14.601168712535287 6 32.48046259619895 36.197112900678476 17.212557905872846 14.109866597249724 7 28.902818132107534 32.479788003196774 20.36937538243471 18.24801848226098 8 26.164803864068713 36.010409366842886 19.34891457985951 18.47587218922889 9 26.13754237039283 17.078512308147733 20.14287086147114 36.6410744599883 10-11 25.01698387087814 26.830448588473665 27.14756682240506 21.00500071824314 12-13 25.66947403174079 23.87965975116248 27.831564444663137 22.61930177243359 14-15 22.449221330910056 25.536735046160626 26.053638782462734 25.960404840466587 16-17 22.140840722151854 28.741154846144322 26.7884155071539 22.329588924549924 18-19 21.957527979268544 27.248782804845405 29.130165031405316 21.663524184480735 20-21 22.631940470738936 26.439859240217807 29.28844723770038 21.63975305134288 22-23 22.26182700419597 26.39845113446702 29.15037392293291 22.189347938404104 24-25 22.14464623152508 26.52708014720413 29.298824065351397 22.029449555919395 26-27 21.964472325885886 26.886863300140018 29.04941220074082 22.099252173233275 28-29 21.875761397248773 26.997946062718103 29.201146966831455 21.925145573201675 30-31 22.134705905228415 26.70461715259849 29.070672743823707 22.09000419834939 32-33 21.87613837569116 26.74673953329275 29.020078268661027 22.35704382235506 34-35 22.336999126392183 26.958291228664017 28.811062021789997 21.893647623153804 36-37 22.47485971442858 26.528735723413206 28.893271848436026 22.10313271372219 38-39 22.048421307438794 26.851642780752904 28.892821924114028 22.207113987694274 40-41 22.404564362692625 26.679497570891574 28.92197586665648 21.99396219975932 42-43 22.188287501619524 26.72749123077482 28.64478641066224 22.43943485694343 44-45 22.19339736354064 27.038023879681354 28.636391504235004 22.132187252542998 46-47 22.463135935981853 27.112733134116564 28.145595567998942 22.27853536190264 48-49 22.41164445761888 27.148529690997908 28.081553742482978 22.358272108900227 50-51 22.525815588061686 27.3400495032316 27.89133098217784 22.24280392652887 52-53 22.625734736090237 27.276776635268323 27.799447428857704 22.298041199783732 54-55 22.392856774381972 27.293953503875734 27.88763502772577 22.42555469401652 56-57 22.316820293184357 27.55507548853312 27.749974851375097 22.378129366907427 58-59 22.361799772617694 27.152899719641187 27.899174003019862 22.586126504721257 60-61 22.342267589596034 27.26274538748609 28.092540553753654 22.302446469164227 62-63 22.294459733214815 27.218477741115592 28.153376679808172 22.333685845861417 64-65 22.070326334429545 27.177135260884018 28.11364910244391 22.638889302242525 66-67 22.10450752749274 27.372224684715928 27.848905707037495 22.67436208075383 68-69 22.242713840731383 27.181323971959532 28.00971666473876 22.566245522570334 70-71 22.2518866778993 27.151951676919158 27.833350132021817 22.762811513159722 72-73 22.225180731401668 27.199232709982944 27.7828548617124 22.792731696902983 74-75 22.160805575473695 27.46955699459968 27.556797777175497 22.81283965275113 76-77 22.22862329774437 27.242833751713025 27.71884964450491 22.809693306037698 78-79 22.23694423412999 27.282705485159735 27.739960857702034 22.740389423008242 80-81 22.194365477318577 27.38839841970512 27.60992303284556 22.807313070130743 82-83 22.25853340306773 27.372503906687122 27.591191085213794 22.77777160503135 84-85 22.2166887959626 27.23280363232618 27.54718381230759 23.003323759403628 86-87 22.18656159958749 27.1416335849175 27.595680840473076 23.07612397502194 88-89 22.166593108239237 27.181279566106188 27.52954021468439 23.12258711097019 90-91 22.06592825860346 27.20676294610054 27.612504693229432 23.11480410206657 92-93 22.09538110139609 27.029969389350477 27.596786238937664 23.277863270315766 94-95 21.870146402554248 26.984454202674723 27.707419491293383 23.437979903477647 96-97 21.99373580874574 26.747592695030793 27.668332779108884 23.590338717114584 98-99 21.795332207687593 26.80425073461281 27.525907378270542 23.874509679429057 100-101 21.886024792160253 26.264701954996177 28.16896462165835 23.680308631185216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 429.0 1 298.0 2 239.0 3 329.0 4 294.0 5 276.0 6 493.0 7 1107.5 8 1807.0 9 2081.5 10 1942.0 11 1786.5 12 1855.0 13 1969.0 14 2118.5 15 2360.5 16 2602.5 17 2811.0 18 3078.0 19 3422.5 20 3941.5 21 4623.0 22 5582.5 23 7046.0 24 8615.5 25 10734.0 26 14032.5 27 18052.0 28 21718.5 29 25698.0 30 30286.5 31 35491.0 32 42057.5 33 46814.0 34 50061.0 35 54601.0 36 61123.5 37 66866.0 38 70532.0 39 73015.0 40 75870.5 41 80418.5 42 83593.5 43 86158.0 44 88911.0 45 92265.5 46 93203.0 47 93111.0 48 95048.5 49 94341.5 50 91979.5 51 89988.5 52 87988.0 53 86828.0 54 85037.0 55 78345.5 56 70054.5 57 63381.5 58 58098.5 59 51669.0 60 42660.0 61 35423.5 62 29314.0 63 21966.5 64 15666.0 65 11568.5 66 8498.0 67 6807.5 68 5243.5 69 3753.0 70 2857.0 71 2095.5 72 1598.5 73 1265.5 74 986.0 75 762.0 76 527.5 77 311.0 78 176.0 79 107.5 80 71.0 81 40.5 82 23.5 83 18.5 84 13.0 85 7.5 86 5.0 87 3.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.293805093415258 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013095040630339702 16-17 5.555471782568358E-4 18-19 9.920485326014926E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.920485326014926E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0011309353271657014 36-37 0.007063385552122626 38-39 0.0028967817151963585 40-41 0.003194396274976806 42-43 1.3888679456420895E-4 44-45 0.0011706172684697613 46-47 9.920485326014926E-5 48-49 2.5793261847638805E-4 50-51 1.984097065202985E-5 52-53 1.984097065202985E-5 54-55 0.0 56-57 7.737978554291641E-4 58-59 0.002103142889115164 60-61 7.341159141251044E-4 62-63 0.0013095040630339702 64-65 1.984097065202985E-5 66-67 3.571374717365373E-4 68-69 0.0014880727989022389 70-71 0.0 72-73 0.0 74-75 3.96819413040597E-5 76-77 0.26076987727962836 78-79 7.93638826081194E-5 80-81 7.93638826081194E-5 82-83 0.0 84-85 0.0 86-87 0.1106729342970225 88-89 0.37943872274941887 90-91 0.2821187617012124 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1904582391217909E-4 100-101 2.9761455978044777E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2520038.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.47892213434223 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8695067467202 41.536795350379 2 14.286488600761334 15.851979773096117 3 4.818842646751626 8.02032587930343 4 2.1206445278172414 4.706042905335668 5 1.0868360230386245 3.014824554747901 6 0.6276819932187709 2.089387225614775 7 0.3990530029436706 1.5497321334451135 8 0.29122583690963905 1.2925516423534809 9 0.20857090543076343 1.041416011969432 >10 1.161435425403535 11.889468318608849 >50 0.08801037095255147 3.338968207557159 >100 0.03833183604052931 3.6659205365273846 >500 0.0027263657966636865 0.9891335574225416 >1k 5.022252783301919E-4 0.4840584785418855 >5k 1.4349293666576912E-4 0.5293954250972024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7150 0.28372588032402685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6150 0.24404393901996715 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4032 0.1599975873379687 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.190458239121791E-4 0.0 4 0.0 0.0 0.0 1.190458239121791E-4 0.0 5 0.0 0.0 0.0 1.190458239121791E-4 0.0 6 3.96819413040597E-5 0.0 0.0 1.190458239121791E-4 0.0 7 3.96819413040597E-5 0.0 0.0 1.587277652162388E-4 0.0 8 3.96819413040597E-5 0.0 0.0 1.587277652162388E-4 0.0 9 3.96819413040597E-5 0.0 0.0 1.587277652162388E-4 0.0 10-11 3.96819413040597E-5 0.0 0.0 2.1825067717232838E-4 0.0 12-13 3.96819413040597E-5 0.0 0.0 2.380916478243582E-4 0.0 14-15 1.3888679456420895E-4 0.0 0.0 2.380916478243582E-4 0.0 16-17 1.587277652162388E-4 0.0 0.0 2.380916478243582E-4 0.0 18-19 2.1825067717232838E-4 0.0 0.0 2.380916478243582E-4 0.0 20-21 2.380916478243582E-4 0.0 0.0 2.5793261847638805E-4 0.0 22-23 2.380916478243582E-4 0.0 0.0 3.7697844238856717E-4 3.96819413040597E-5 24-25 2.380916478243582E-4 0.0 0.0 6.150700902129254E-4 3.96819413040597E-5 26-27 2.380916478243582E-4 0.0 0.0 8.531617380372836E-4 3.96819413040597E-5 28-29 2.9761455978044777E-4 0.0 0.0 0.002301552595635463 3.96819413040597E-5 30-31 3.571374717365373E-4 0.0 0.0 0.006825293904298269 3.96819413040597E-5 32-33 3.571374717365373E-4 0.0 0.0 0.014900568959674418 3.96819413040597E-5 34-35 3.571374717365373E-4 0.0 0.0 0.027102765910672774 3.96819413040597E-5 36-37 3.571374717365373E-4 0.0 0.0 0.04974131342463883 3.96819413040597E-5 38-39 3.9681941304059703E-4 0.0 0.0 0.08370905518091393 3.96819413040597E-5 40-41 3.9681941304059703E-4 0.0 0.0 0.12063310156434148 3.96819413040597E-5 42-43 4.3650135434465675E-4 0.0 0.0 0.1596007679249281 3.96819413040597E-5 44-45 4.761832956487164E-4 0.0 0.0 0.21053253958868873 3.96819413040597E-5 46-47 5.753881489088657E-4 0.0 0.0 0.2714046375491163 3.96819413040597E-5 48-49 5.952291195608955E-4 0.0 0.0 0.34005439600513965 3.96819413040597E-5 50-51 5.952291195608955E-4 0.0 0.0 0.41548976642415714 3.96819413040597E-5 52-53 5.952291195608955E-4 0.0 0.0 0.4908457729605665 7.93638826081194E-5 54-55 6.54752031516985E-4 0.0 0.0 0.5786023861544944 7.93638826081194E-5 56-57 7.142749434730746E-4 0.0 0.0 0.6838984174048168 7.93638826081194E-5 58-59 7.142749434730746E-4 0.0 0.0 0.8032220149061244 7.93638826081194E-5 60-61 7.142749434730746E-4 0.0 0.0 0.9353430384779913 1.190458239121791E-4 62-63 7.142749434730746E-4 0.0 0.0 1.07413062818894 1.190458239121791E-4 64-65 7.142749434730746E-4 0.0 0.0 1.2191284417139743 1.190458239121791E-4 66-67 7.539568847771343E-4 0.0 0.0 1.393054390449668 1.190458239121791E-4 68-69 7.539568847771343E-4 0.0 0.0 1.5874760618689083 1.190458239121791E-4 70-71 7.737978554291642E-4 0.0 0.0 1.8056275341879766 1.190458239121791E-4 72-73 7.936388260811941E-4 0.0 0.0 2.0325685565058937 1.190458239121791E-4 74-75 7.936388260811941E-4 0.0 0.0 2.270263384917212 1.190458239121791E-4 76-77 7.936388260811941E-4 0.0 0.0 2.5355371625348506 1.190458239121791E-4 78-79 8.134797967332239E-4 0.0 0.0 2.84108811057611 1.190458239121791E-4 80-81 8.531617380372835E-4 0.0 0.0 3.1674323958606974 1.190458239121791E-4 82-83 9.126846499933731E-4 0.0 0.0 3.5100065951386448 1.190458239121791E-4 84-85 9.325256206454029E-4 0.0 0.0 3.858017220375248 1.190458239121791E-4 86-87 9.523665912974328E-4 0.0 0.0 4.2328925198747 1.190458239121791E-4 88-89 9.523665912974328E-4 0.0 0.0 4.649671949391239 1.190458239121791E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4895 0.0 46.77611 1 GTATCAA 8205 0.0 37.942936 1 ATCAACG 10555 0.0 28.954872 3 TATCAAC 10620 0.0 28.867025 2 TCAACGC 10720 0.0 28.46494 4 CAACGCA 10850 0.0 28.167624 5 AACGCAG 11315 0.0 27.05199 6 GTACCGT 295 0.0 24.130331 6 GTACATG 9585 0.0 23.739277 1 TACATGG 9555 0.0 23.392912 2 ACGCAGA 13145 0.0 23.1776 7 CGCAGAG 13305 0.0 22.827541 8 ACATGGG 9750 0.0 22.000263 3 GTGTAGC 1660 0.0 20.646961 1 TATACCG 115 0.0037443973 20.633183 5 GCAGAGT 14805 0.0 20.322798 9 GAGTACT 8510 0.0 20.076723 12-13 GGACCGA 480 0.0 19.773466 6 GTACTTT 8855 0.0 19.08013 14-15 AGTACTT 9000 0.0 19.03639 12-13 >>END_MODULE