FastQCFastQC Report
Fri 27 May 2016
SRR939320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565624
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73040.2846870780753532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58220.22692335275940667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.14967119110204768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.044.7161871
GTATCAA84200.037.4576071
ATCAACG101600.030.942653
TCAACGC103600.030.299534
CAACGCA103650.030.2849145
TATCAAC106950.029.4391292
AACGCAG108000.029.0914126
ACGCAGA121550.025.6144737
CGCAGAG122850.025.3819968
GTACATG93200.023.443691
TACATGG91500.023.164562
GCAGAGT136250.022.6070259
GTACCGT2103.5372068E-822.566066
ACATGGG93400.021.5764353
GAGTACT74800.021.41445712-13
GTCCTAC6200.020.6850281
AGAGTAC121600.019.62151110-11
GTACTTT81200.019.58128514-15
CAGAGTA130100.019.46870210-11
AGTACTT80200.018.61350412-13
Produced by FastQC (version 0.11.5)