##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939320_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2565624 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824104389419492 33.0 31.0 34.0 30.0 34.0 2 31.9065147504077 33.0 31.0 34.0 30.0 34.0 3 31.9595096553509 34.0 31.0 34.0 30.0 34.0 4 35.44857898117573 37.0 35.0 37.0 33.0 37.0 5 35.49536876798783 37.0 35.0 37.0 33.0 37.0 6 35.578914525277284 37.0 35.0 37.0 33.0 37.0 7 35.49524949875742 37.0 35.0 37.0 33.0 37.0 8 35.47329928313736 37.0 35.0 37.0 33.0 37.0 9 37.10101830977571 39.0 37.0 39.0 33.0 39.0 10-11 37.01454655865396 39.0 37.0 39.0 33.0 39.0 12-13 36.913678699606805 39.0 37.0 39.0 33.0 39.0 14-15 38.15508858663623 40.0 38.0 41.0 33.0 41.0 16-17 38.08756115471324 40.0 38.0 41.0 33.0 41.0 18-19 38.024009558688256 40.0 38.0 41.0 33.0 41.0 20-21 37.92231597459332 40.0 38.0 41.0 32.5 41.0 22-23 37.84398746659682 40.0 38.0 41.0 32.0 41.0 24-25 37.870328621808966 40.0 38.0 41.0 32.0 41.0 26-27 37.78874885797763 40.0 38.0 41.0 32.0 41.0 28-29 37.65457428680118 40.0 38.0 41.0 32.0 41.0 30-31 37.4741977000527 40.0 37.0 41.0 32.0 41.0 32-33 37.274287853559215 40.0 37.0 41.0 31.0 41.0 34-35 36.98061836028974 40.0 36.5 41.0 30.5 41.0 36-37 36.7372535882109 40.0 36.0 41.0 30.0 41.0 38-39 36.417328883733546 39.5 35.5 41.0 30.0 41.0 40-41 36.28685399731216 39.0 35.0 41.0 30.0 41.0 42-43 36.127746700217955 39.0 35.0 41.0 29.5 41.0 44-45 36.10257894375793 39.0 35.0 41.0 29.0 41.0 46-47 35.931195685727914 39.0 35.0 41.0 27.5 41.0 48-49 35.73852501379781 39.0 35.0 41.0 27.0 41.0 50-51 34.60124612959655 38.0 34.0 40.0 25.0 40.5 52-53 34.70930268815695 38.5 34.5 39.5 25.5 40.5 54-55 35.21288329856596 39.0 35.0 40.5 26.0 41.0 56-57 35.10932135807897 39.0 35.0 41.0 26.0 41.0 58-59 34.909137309286166 38.5 35.0 41.0 26.0 41.0 60-61 34.62552638266558 38.0 34.0 40.0 25.0 41.0 62-63 34.272886634986264 38.0 34.0 40.0 23.5 41.0 64-65 33.90082763491455 37.0 34.0 40.0 23.0 41.0 66-67 33.55194603729931 37.0 33.5 39.5 22.0 41.0 68-69 33.15161886543001 36.0 33.0 39.0 21.5 41.0 70-71 32.747943385312894 35.5 33.0 39.0 20.0 40.5 72-73 32.314123971400335 35.0 32.0 38.5 18.5 40.0 74-75 31.873931838804126 35.0 32.0 37.5 18.0 39.5 76-77 31.431646453260495 35.0 31.0 37.0 14.5 39.0 78-79 31.00374860073027 35.0 31.0 36.0 10.0 39.0 80-81 30.553928790812684 35.0 31.0 36.0 7.0 37.5 82-83 30.120711374698708 34.0 30.0 35.5 4.0 37.0 84-85 29.70628120098658 34.0 30.0 35.0 2.0 37.0 86-87 29.381620806478267 34.0 30.0 35.0 2.0 36.0 88-89 28.98255239271226 34.0 29.0 35.0 2.0 36.0 90-91 28.59224247200681 34.0 29.0 35.0 2.0 36.0 92-93 28.192665215167928 34.0 29.0 35.0 2.0 35.0 94-95 27.741102359503962 34.0 27.5 35.0 2.0 35.0 96-97 27.19966370754249 34.0 26.5 35.0 2.0 35.0 98-99 26.489960337134356 33.5 25.0 35.0 2.0 35.0 100-101 24.048071151501546 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3444820968968223 1101 2 0.13981277864682085 1101 3 0.3503580052995936 1101 4 0.2808645695579486 1101 5 0.28100304189255354 1101 6 0.3163879324259753 1101 7 0.264764342443776 1101 8 0.18101844700208147 1101 9 0.34975920844097885 1101 10-11 0.27025980310326503 1101 12-13 0.3636824048698628 1101 14-15 0.40480311085701004 1101 16-17 0.49146871923813507 1101 18-19 0.5226712035916066 1101 20-21 0.45615958556948755 1101 22-23 0.45929149479426457 1101 24-25 0.5186464561110213 1101 26-27 0.45192123703892406 1101 28-29 0.43066398763391334 1101 30-31 0.49631472763321227 1101 32-33 0.44916898487293366 1101 34-35 0.43116415395624585 1101 36-37 0.4511449757511059 1101 38-39 0.515305026264187 1101 40-41 0.6049607831677761 1101 42-43 0.5408420407678918 1101 44-45 0.6011047215255374 1101 46-47 0.6422293950257227 1101 48-49 0.6250869578728171 1101 50-51 0.6537092805727625 1101 52-53 0.6740192884052618 1101 54-55 0.8344824139111395 1101 56-57 0.6164560414249323 1101 58-59 0.7035386405563031 1101 60-61 0.7338360119962033 1101 62-63 0.8145387270898468 1101 64-65 0.7719150695325396 1101 66-67 0.6048452210192465 1101 68-69 0.6738385238714883 1101 70-71 0.6214473392239697 1101 72-73 0.6479524162992192 1101 74-75 0.5339864566412871 1101 76-77 0.5792298202868444 1101 78-79 0.5233102879506788 1101 80-81 0.3872015707829064 1101 82-83 0.20949086942928474 1101 84-85 0.15843361209635276 1101 86-87 0.25578064282291635 1101 88-89 0.20372080621371325 1101 90-91 0.2445667784357859 1101 92-93 0.2924282883125109 1101 94-95 0.38951602556462817 1101 96-97 0.30502918018633096 1101 98-99 0.27880031267789107 1101 100-101 0.07816418663341906 1102 1 0.33232136601288076 1102 2 0.36690741014691497 1102 3 0.3556069496766803 1102 4 0.3778296853847678 1102 5 0.3297350261254266 1102 6 0.3605885309364041 1102 7 0.40435289329658985 1102 8 0.433981828963681 1102 9 0.413938150546457 1102 10-11 0.47016161086632025 1102 12-13 0.5271271035318605 1102 14-15 0.5695552543732489 1102 16-17 0.5899771780314964 1102 18-19 0.5253548616864592 1102 20-21 0.5961537007993698 1102 22-23 0.5021978948199646 1102 24-25 0.4836918235173968 1102 26-27 0.5164651588999973 1102 28-29 0.5984963348173054 1102 30-31 0.5949094287472008 1102 32-33 0.4908744746757705 1102 34-35 0.6189855545653487 1102 36-37 0.5326191670320668 1102 38-39 0.6867254485584482 1102 40-41 0.6201022290287312 1102 42-43 0.5743728295581931 1102 44-45 0.5754431217361216 1102 46-47 0.5435202678261959 1102 48-49 0.5144741357253366 1102 50-51 0.3627984320216058 1102 52-53 0.4010428968177777 1102 54-55 0.4406515018450534 1102 56-57 0.5129001521736001 1102 58-59 0.49630764472806277 1102 60-61 0.4949290009464775 1102 62-63 0.4140074067537469 1102 64-65 0.5101673717233766 1102 66-67 0.5234832011226374 1102 68-69 0.5442459089197698 1102 70-71 0.43470565864861754 1102 72-73 0.2999071576821777 1102 74-75 0.1302024479967656 1102 76-77 0.2164092229150434 1102 78-79 0.09366626581176973 1102 80-81 0.1748681861551198 1102 82-83 -0.0422465187586738 1102 84-85 -0.041208341354213474 1102 86-87 -0.06419865902013555 1102 88-89 -0.09420836347566208 1102 90-91 -0.29777679180464034 1102 92-93 -0.19920648842415645 1102 94-95 -0.1540915865320649 1102 96-97 -0.06614423959224425 1102 98-99 -0.13178866600447137 1102 100-101 -0.4653715568627561 1103 1 0.23317306256091896 1103 2 0.342127561693097 1103 3 0.28415128045962845 1103 4 0.28502639438633537 1103 5 0.23650312644895877 1103 6 0.20799750008590223 1103 7 0.19619810028399343 1103 8 0.3755026698275046 1103 9 0.3774260254718058 1103 10-11 0.30924236648588277 1103 12-13 0.3516500852009159 1103 14-15 0.4519061822979822 1103 16-17 0.39844495982679007 1103 18-19 0.4343955392238996 1103 20-21 0.4731357361646218 1103 22-23 0.5741219001512761 1103 24-25 0.5452336322609455 1103 26-27 0.4059560210301285 1103 28-29 0.38931496993181014 1103 30-31 0.41696623189346127 1103 32-33 0.42488086165382555 1103 34-35 0.33509805147388505 1103 36-37 0.29557432128456185 1103 38-39 0.5511988436726085 1103 40-41 0.4360427788788286 1103 42-43 0.35772671671063705 1103 44-45 0.34482992409530056 1103 46-47 0.38064104333558646 1103 48-49 0.24572231954735457 1103 50-51 0.31838409090206454 1103 52-53 0.37637747457108617 1103 54-55 0.4031542109577799 1103 56-57 0.37560828756917886 1103 58-59 0.27856632293470796 1103 60-61 0.2806413549108484 1103 62-63 0.31086127357618665 1103 64-65 0.44019299498717146 1103 66-67 0.2575186469483981 1103 68-69 0.13586716880546845 1103 70-71 0.13655124985441347 1103 72-73 0.15117043419967757 1103 74-75 0.17452819506840456 1103 76-77 0.2121041117285749 1103 78-79 0.03664676355737839 1103 80-81 0.15684274791300368 1103 82-83 0.10916854267710008 1103 84-85 0.04184167753963308 1103 86-87 -0.03347396343860609 1103 88-89 0.0023844213504737866 1103 90-91 0.06454479377142874 1103 92-93 0.015588661620586919 1103 94-95 -0.05036371685894636 1103 96-97 -0.09542056237598473 1103 98-99 -0.5273820726675673 1103 100-101 -0.6076054772295763 1104 1 0.16126320411534678 1104 2 0.2033897428735898 1104 3 0.23837489634746234 1104 4 0.2623640038163586 1104 5 0.2860007772765272 1104 6 0.21609687748515682 1104 7 0.2676035898983997 1104 8 0.1797285964960622 1104 9 0.239510808198375 1104 10-11 0.33177047704236884 1104 12-13 0.269148845203226 1104 14-15 0.19986214209257724 1104 16-17 0.3311110840361593 1104 18-19 0.2012354901843949 1104 20-21 0.3313686111870524 1104 22-23 0.277335049840012 1104 24-25 0.3624709829199162 1104 26-27 0.3337329335286867 1104 28-29 0.2971315587172825 1104 30-31 0.3456563576545193 1104 32-33 0.36346449499442457 1104 34-35 0.4475834908260836 1104 36-37 0.44402724449495423 1104 38-39 0.701677884891474 1104 40-41 0.5619251968943288 1104 42-43 0.44562799651925644 1104 44-45 0.4163068295705443 1104 46-47 0.4721888079008778 1104 48-49 0.553270493866826 1104 50-51 0.5220357430738929 1104 52-53 0.5879582887592036 1104 54-55 0.5500327101075158 1104 56-57 0.557559161462784 1104 58-59 0.7311018862138923 1104 60-61 0.6898519631046938 1104 62-63 0.5811623009820437 1104 64-65 0.5989370802073068 1104 66-67 0.5511644730415952 1104 68-69 0.500798305720501 1104 70-71 0.3611020031507337 1104 72-73 0.3623483173717048 1104 74-75 0.2643509709237648 1104 76-77 0.31958962204175734 1104 78-79 0.37486154534871474 1104 80-81 0.27993676320928174 1104 82-83 0.3385924959753126 1104 84-85 0.34365684128301055 1104 86-87 0.45408484775023084 1104 88-89 0.34259639090347704 1104 90-91 0.2131859455373224 1104 92-93 0.2836209390423683 1104 94-95 0.16034027431423326 1104 96-97 0.2314073470516007 1104 98-99 0.16132310409988193 1104 100-101 0.12294584593801972 1105 1 0.1646563745917966 1105 2 0.11496799261552582 1105 3 0.2171220325874934 1105 4 0.2528426474097003 1105 5 0.25418230095130667 1105 6 0.1456319195617084 1105 7 0.1961307438245825 1105 8 0.30872804020677336 1105 9 0.2695038223630064 1105 10-11 0.266032565741817 1105 12-13 0.2607867631947798 1105 14-15 0.27658734065573043 1105 16-17 0.3058542967683593 1105 18-19 0.3735238093980584 1105 20-21 0.27975949453948346 1105 22-23 0.32678627248478875 1105 24-25 0.3047213222810967 1105 26-27 0.2804941417162894 1105 28-29 0.30485358143528885 1105 30-31 0.3114521576438918 1105 32-33 0.15626059281306226 1105 34-35 0.37165247422903036 1105 36-37 0.3688086701173461 1105 38-39 0.43792252436180945 1105 40-41 0.42978115627797564 1105 42-43 0.15159238517744456 1105 44-45 0.15826724881952714 1105 46-47 0.3372325139492176 1105 48-49 0.2125511593801903 1105 50-51 0.10462466506080403 1105 52-53 0.21676009176500344 1105 54-55 0.2973009165768232 1105 56-57 0.24425777430573703 1105 58-59 0.31702315936252035 1105 60-61 0.28043540305572634 1105 62-63 0.2752005428254165 1105 64-65 0.14622881995572357 1105 66-67 0.3141116163786819 1105 68-69 0.27821276631143377 1105 70-71 0.16490524940151374 1105 72-73 0.21211719514852945 1105 74-75 0.22612360413880594 1105 76-77 0.15806490918571825 1105 78-79 0.23841204453493603 1105 80-81 0.313419428998408 1105 82-83 0.29747625587654625 1105 84-85 0.18208640360109385 1105 86-87 0.20283425610805494 1105 88-89 0.16682618798941107 1105 90-91 0.09217457594461465 1105 92-93 0.17831816566395986 1105 94-95 0.17784908693584 1105 96-97 0.10480165976597888 1105 98-99 0.07805651900818944 1105 100-101 -0.10731925742215509 1106 1 0.2171678856843009 1106 2 0.2878406346981919 1106 3 0.2960974103268619 1106 4 0.29253043012348456 1106 5 0.33807149740277964 1106 6 0.3645479189675882 1106 7 0.33362956233414565 1106 8 0.4178017179501978 1106 9 0.38328793367966085 1106 10-11 0.47162894835567926 1106 12-13 0.4003765665947796 1106 14-15 0.510106730601585 1106 16-17 0.5097537215512986 1106 18-19 0.5564113551386214 1106 20-21 0.5777269090333377 1106 22-23 0.5108845083507774 1106 24-25 0.5094340344430179 1106 26-27 0.5648723908149833 1106 28-29 0.5064913803523652 1106 30-31 0.4004433673550736 1106 32-33 0.4619612841537517 1106 34-35 0.5549084762004313 1106 36-37 0.5831851301019526 1106 38-39 0.8063898105795602 1106 40-41 0.7363104943732779 1106 42-43 0.5711058811738852 1106 44-45 0.6938098567402378 1106 46-47 0.620615019789156 1106 48-49 0.616495058838737 1106 50-51 0.6194873083587211 1106 52-53 0.6590966916380765 1106 54-55 0.7504558336024374 1106 56-57 0.8190288183381966 1106 58-59 0.7615115064911677 1106 60-61 0.80984565366672 1106 62-63 0.7452584527350794 1106 64-65 0.7821430910098783 1106 66-67 0.6761465311088273 1106 68-69 0.6861926370901443 1106 70-71 0.6839104513351089 1106 72-73 0.726005699584185 1106 74-75 0.6356671775059937 1106 76-77 0.703220967193527 1106 78-79 0.6340217948691311 1106 80-81 0.705976630013815 1106 82-83 0.7527678983507897 1106 84-85 0.7629981597684719 1106 86-87 0.7315847287042274 1106 88-89 0.7474970045005733 1106 90-91 0.7001151199703237 1106 92-93 0.7731718465130122 1106 94-95 0.6412052455391155 1106 96-97 0.5913928404462467 1106 98-99 0.6059870372436471 1106 100-101 0.4062732836599565 1107 1 0.20298277502032036 1107 2 0.2620682585055505 1107 3 0.21386936463335005 1107 4 0.21941512539186903 1107 5 0.23780862987503326 1107 6 0.12240473729691814 1107 7 0.27079286472915953 1107 8 0.11982433753869515 1107 9 0.2836976500423489 1107 10-11 0.31286913138936967 1107 12-13 0.30998542944931984 1107 14-15 0.39605711029911816 1107 16-17 0.2914154662128112 1107 18-19 0.3387055233738394 1107 20-21 0.3294116140375536 1107 22-23 0.44113658532878475 1107 24-25 0.36960514173191683 1107 26-27 0.4828191844801992 1107 28-29 0.5131624551884713 1107 30-31 0.4451113754033571 1107 32-33 0.4586097231749804 1107 34-35 0.47815562035568604 1107 36-37 0.5770592619900583 1107 38-39 0.6871749244888221 1107 40-41 0.6909459685084087 1107 42-43 0.6267721367719901 1107 44-45 0.719905138105851 1107 46-47 0.5282852346265656 1107 48-49 0.4794822562165493 1107 50-51 0.45057950671012037 1107 52-53 0.5597145114602355 1107 54-55 0.5915150923809165 1107 56-57 0.6478927276002224 1107 58-59 0.5781914247101554 1107 60-61 0.5173734819266045 1107 62-63 0.6222206290185923 1107 64-65 0.6678330371382657 1107 66-67 0.5247303728690724 1107 68-69 0.5013509255304669 1107 70-71 0.39744447807620986 1107 72-73 0.4701094348993067 1107 74-75 0.6291157723005654 1107 76-77 0.48072064266571246 1107 78-79 0.4429260267117989 1107 80-81 0.411433970832924 1107 82-83 0.4934137898286899 1107 84-85 0.4698347437887236 1107 86-87 0.5526072347079918 1107 88-89 0.529265032020529 1107 90-91 0.5240072631519901 1107 92-93 0.6136278892208757 1107 94-95 0.40396770740710153 1107 96-97 0.4869058377851232 1107 98-99 0.6214098158507788 1107 100-101 0.4863310496104951 1108 1 0.14426415897622746 1108 2 0.25404730670780395 1108 3 0.10601025344447734 1108 4 0.2327954070820084 1108 5 0.3634045969820221 1108 6 0.2446971876512194 1108 7 0.28018836818467463 1108 8 0.2586802993968007 1108 9 0.39584553736629147 1108 10-11 0.2905930837994859 1108 12-13 0.322875815242341 1108 14-15 0.2756209061175099 1108 16-17 0.2605476506236286 1108 18-19 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-1.5559136267945703 2316 74-75 -1.5663549022439796 2316 76-77 -1.3451766080183738 2316 78-79 -1.3634816203253806 2316 80-81 -1.2373233146186386 2316 82-83 -1.1827976656220116 2316 84-85 -1.2005606152116037 2316 86-87 -1.0424627036904397 2316 88-89 -1.2364433020390067 2316 90-91 -1.1492304951269077 2316 92-93 -1.1946696341290455 2316 94-95 -1.3205512381559181 2316 96-97 -1.1815412902832563 2316 98-99 -1.0811858673275374 2316 100-101 -0.6114334649713804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8671.0 3 2567.0 4 2497.0 5 2718.0 6 3063.0 7 3786.0 8 4641.0 9 5781.0 10 7059.0 11 8513.0 12 10765.0 13 14755.0 14 13951.0 15 14677.0 16 17358.0 17 21980.0 18 18790.0 19 14893.0 20 14847.0 21 15594.0 22 17194.0 23 18966.0 24 21129.0 25 24583.0 26 27897.0 27 32855.0 28 38495.0 29 45752.0 30 54724.0 31 66586.0 32 82947.0 33 108112.0 34 145751.0 35 210440.0 36 327618.0 37 521047.0 38 520235.0 39 94309.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.527027993607135 18.623227803103173 14.37171410960505 23.47803009368464 2 15.203530147621446 19.80978906595381 40.97405260338242 24.012628183042324 3 19.384016826857412 23.924809858852637 29.437556827715927 27.25361648657402 4 12.747062257659673 16.24491904818397 37.16006012849891 33.84795856565745 5 13.876978206410579 37.080128116317816 34.27248094431732 14.770412732954282 6 32.374567592126766 35.8171387566153 17.57028699792912 14.23800665332881 7 28.989552256852154 32.26990256651675 20.489659710215246 18.250885466415852 8 26.109396196712513 35.53734287860668 19.675424821611198 18.677836103069605 9 26.314397655093597 17.06882799534295 20.23035624089506 36.386418108668394 10-11 24.987582769349576 26.574804186074175 27.334249228006097 21.103363816570152 12-13 25.746876411436116 23.750194041994195 27.835021791208476 22.667907755361213 14-15 22.54299324272805 25.35520481158481 26.123385980030644 25.9784159656565 16-17 22.207420876177284 28.53641014120367 26.84186078076223 22.414308201856816 18-19 22.05156848133859 27.121183961438994 29.07942344929965 21.747824107922764 20-21 22.750115018163996 26.3529968399503 29.215491284070776 21.681396857814924 22-23 22.35601510124057 26.318179300315432 29.060584404590916 22.265221193853087 24-25 22.148822814684138 26.437753104254618 29.275212602209695 22.13821147885155 26-27 21.96989454371034 26.759833409374945 28.976321246585684 22.29395080032903 28-29 21.92738146624658 26.9371018162533 29.11966026854966 22.015856448950462 30-31 22.152973321836203 26.661039028670725 28.975106088712927 22.210881560780148 32-33 21.87441613186458 26.685080006755808 28.95790922647199 22.48259463490762 34-35 22.411394651612053 26.837877124722333 28.723512945883765 22.02721527778185 36-37 22.492032026412893 26.497622076983486 28.766990927414426 22.2433549691892 38-39 22.08665780748567 26.79805134518503 28.792090560748612 22.32320028658069 40-41 22.45100287530086 26.625027883986196 28.80022318919292 22.123746051520026 42-43 22.217113969082053 26.662030780277952 28.600928989393065 22.51992626124693 44-45 22.206519643065988 26.98858735851544 28.57024952758619 22.23464347083238 46-47 22.484033565452062 27.042474344076982 28.06706831558785 22.406423774883102 48-49 22.38942020673537 27.030618304788746 28.130900060169367 22.449061428306518 50-51 22.528560450043102 27.260596759716282 27.895755897661783 22.31508689257883 52-53 22.54879006964603 27.183252561775717 27.89248532097477 22.375472047603484 54-55 22.342657930731825 27.224078606203527 27.945043559073362 22.48821990399129 56-57 22.340219398623702 27.43497571090453 27.824441996036708 22.400362894435062 58-59 22.361039537878902 27.07599083202286 27.963249111080007 22.599720519018234 60-61 22.30606737445211 27.13817840023293 28.164740872752997 22.391013352561963 62-63 22.26663621284872 27.147453710131543 28.25202385566455 22.333886221355193 64-65 22.1206145078081 27.110077081459565 28.111650946920868 22.65765746381146 66-67 22.099238171807954 27.28995930200145 27.918298841266072 22.692503684924525 68-69 22.210292578426866 27.05426053272555 28.159661152901815 22.575785735945768 70-71 22.255561694348625 27.04807222768279 27.964358828280044 22.73200724968854 72-73 22.206738606795412 27.14796368070664 27.899966628940025 22.745331083557925 74-75 22.107730792734692 27.416259069700843 27.77675840500024 22.69925173256423 76-77 22.262006232879042 27.177058123337776 27.8006980793235 22.760237564459683 78-79 22.279830739809388 27.18280335235294 27.83968384949609 22.697682058341577 80-81 22.269370691450412 27.373157179995328 27.698140730391728 22.659331398162532 82-83 22.326414407282495 27.32127693943811 27.73198102089915 22.620327632380246 84-85 22.305575988169792 27.24714370958963 27.630381538243693 22.816898763996885 86-87 22.268323415253562 27.18222689492233 27.761686036403642 22.787763653420463 88-89 22.336804711367332 27.133823667192104 27.665826058943725 22.863545562496835 90-91 22.288814605578708 27.137835277563983 27.79595732018198 22.77739279667533 92-93 22.286446513811722 26.962966585804836 27.85337272087018 22.897214179513263 94-95 22.179063610945693 26.90968214481626 27.910868457769684 23.000385786468364 96-97 22.339711233712876 26.66881871894197 27.89631020855343 23.09515983879172 98-99 22.15710224627401 26.764264469871662 27.79943082184974 23.27920246200458 100-101 22.346283054665843 26.30917589250947 28.240495998206956 23.104045054617732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1951.0 1 1113.5 2 341.0 3 421.0 4 377.5 5 386.0 6 646.5 7 1299.5 8 2140.5 9 2504.0 10 2329.0 11 2183.0 12 2281.0 13 2535.0 14 2777.0 15 2966.0 16 3135.5 17 3313.0 18 3571.5 19 3847.5 20 4217.0 21 4830.0 22 5758.5 23 7204.0 24 8931.5 25 11022.0 26 14193.0 27 18350.0 28 22132.0 29 25693.0 30 30074.0 31 35549.5 32 42266.5 33 47143.0 34 50656.0 35 55592.5 36 62167.5 37 68299.5 38 71933.5 39 74341.5 40 77364.0 41 81388.0 42 84405.5 43 87034.5 44 90231.5 45 93629.0 46 94148.0 47 94121.5 48 96046.0 49 95008.0 50 92797.0 51 91287.5 52 89219.5 53 87682.0 54 85490.5 55 78927.5 56 70927.0 57 64557.0 58 59251.0 59 52408.5 60 43600.5 61 36464.5 62 30011.5 63 22684.5 64 16475.0 65 12354.5 66 9162.5 67 7214.5 68 5666.0 69 4189.5 70 3198.0 71 2326.0 72 1786.5 73 1417.5 74 1049.0 75 806.0 76 600.0 77 376.5 78 230.5 79 158.0 80 99.5 81 60.0 82 57.0 83 57.5 84 39.0 85 21.5 86 20.5 87 18.0 88 9.5 89 8.5 90 9.5 91 7.0 92 4.0 93 3.5 94 3.0 95 4.0 96 4.0 97 2.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2785677090641497 2 0.1085505904216674 3 0.11903536917334731 4 0.12129602778895114 5 0.12725948930942338 6 0.05823144778814043 7 0.06707919788714169 8 0.0717953994817635 9 0.06895008777591728 10-11 0.06417542087227124 12-13 0.06805361970421231 14-15 0.06413644399958841 16-17 0.06501342363495197 18-19 0.06978809053859801 20-21 0.0739975927883431 22-23 0.08286483132368577 24-25 0.090718671169275 26-27 0.09099150927805477 28-29 0.08526190899367951 30-31 0.08128626798003137 32-33 0.07417298871541582 34-35 0.23941544045425206 36-37 0.3623484968958819 38-39 0.33434361387327216 40-41 0.3177589545467302 42-43 0.3414179162652049 44-45 0.3536371658512705 46-47 0.3603606763890578 48-49 0.37051415172293367 50-51 0.36386859493051205 52-53 0.36406347929392613 54-55 0.3500512935644506 56-57 0.3276590802081677 58-59 0.3128283801523528 60-61 0.2704605195461221 62-63 0.3409307053566696 64-65 0.37016335986878823 66-67 0.37423644306414344 68-69 0.38583206268728387 70-71 0.3869623919950858 72-73 0.37094289732244473 74-75 0.37363230153755966 76-77 0.3578466681010156 78-79 0.33574678128985386 80-81 0.33409026420083376 82-83 0.3475567737127498 84-85 0.3553716366856562 86-87 0.3480439846212851 88-89 0.3639855255485605 90-91 0.38754704508532817 92-93 0.38290879723607196 94-95 0.38220721352778114 96-97 0.3868064845043545 98-99 0.37643863637072306 100-101 0.35400744614175733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2565624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.913670593715146 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39569487901292 43.47959413122112 2 13.574563258427125 15.451564434873399 3 4.360661208271773 7.445437067351146 4 1.9305563360423643 4.39500189488499 5 1.0169970310549061 2.894051701012261 6 0.6263681285028654 2.138934560160835 7 0.3832665529279788 1.5269174440052171 8 0.2717273966107855 1.2372002833595226 9 0.20879436529072 1.0694928355181883 >10 1.1081640943703226 11.655882227361095 >50 0.08393580994482161 3.2479858633393395 >100 0.03625220376270693 3.5643083610842696 >500 0.0023326594669557947 0.8671194232923429 >1k 5.488610510417821E-4 0.5139772580126943 >5k 1.3721526276044553E-4 0.5125325145236364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7304 0.2846870780753532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5822 0.22692335275940667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3840 0.14967119110204768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.795374536564983E-5 0.0 4 3.897687268282492E-5 0.0 0.0 7.795374536564983E-5 0.0 5 3.897687268282492E-5 0.0 0.0 7.795374536564983E-5 0.0 6 7.795374536564983E-5 0.0 0.0 7.795374536564983E-5 0.0 7 1.1693061804847475E-4 0.0 0.0 7.795374536564983E-5 0.0 8 1.1693061804847475E-4 0.0 0.0 7.795374536564983E-5 0.0 9 1.1693061804847475E-4 0.0 0.0 1.1693061804847475E-4 0.0 10-11 1.1693061804847475E-4 0.0 0.0 1.1693061804847475E-4 0.0 12-13 1.5590749073129967E-4 0.0 0.0 1.3641905438988722E-4 0.0 14-15 1.948843634141246E-4 0.0 0.0 1.5590749073129967E-4 0.0 16-17 1.948843634141246E-4 0.0 0.0 1.5590749073129967E-4 0.0 18-19 1.948843634141246E-4 0.0 0.0 1.5590749073129967E-4 0.0 20-21 1.948843634141246E-4 0.0 0.0 2.1437279975553705E-4 0.0 22-23 1.948843634141246E-4 0.0 0.0 4.0925716316966165E-4 0.0 24-25 1.948843634141246E-4 0.0 0.0 5.846530902423738E-4 0.0 26-27 1.948843634141246E-4 0.0 0.0 8.185143263393233E-4 0.0 28-29 3.5079185414542424E-4 0.0 0.0 0.001695493961702884 0.0 30-31 4.67722472193899E-4 0.0 0.0 0.005398296866571251 0.0 32-33 5.06699344876724E-4 0.0 0.0 0.01284287954899081 0.0 34-35 6.236299629251987E-4 0.0 0.0 0.02636785436993106 0.0 36-37 6.626068356080236E-4 0.0 0.0 0.04731792343694945 0.0 38-39 7.405605809736735E-4 0.0 0.0 0.07970770463637697 0.0 40-41 7.405605809736735E-4 0.0 0.0 0.1158977309223799 0.0 42-43 7.600490173150859E-4 0.0 0.0 0.15352990149764734 0.0 44-45 8.769796353635607E-4 0.0 0.0 0.2005360099531342 0.0 46-47 9.159565080463856E-4 0.0 0.0 0.26159328101077944 0.0 48-49 9.35444944387798E-4 0.0 0.0 0.32606102842817186 0.0 50-51 9.35444944387798E-4 0.0 0.0 0.39998066747114935 0.0 52-53 9.35444944387798E-4 0.0 0.0 0.47526449705802565 0.0 54-55 9.35444944387798E-4 0.0 0.0 0.5593181229985376 0.0 56-57 9.35444944387798E-4 0.0 0.0 0.6633084193163145 0.0 58-59 9.35444944387798E-4 0.0 0.0 0.7797323380199125 0.0 60-61 9.35444944387798E-4 0.0 0.0 0.909739696853475 0.0 62-63 9.35444944387798E-4 0.0 0.0 1.0447166069540978 0.0 64-65 9.35444944387798E-4 0.0 0.0 1.1923025353676142 7.795374536564983E-5 66-67 9.74421817070623E-4 0.0 0.0 1.3620857927739995 7.795374536564983E-5 68-69 9.74421817070623E-4 0.0 0.0 1.5607509128383583 7.795374536564983E-5 70-71 9.74421817070623E-4 0.0 0.0 1.7813989890958302 7.795374536564983E-5 72-73 0.001013398689753448 0.0 0.0 2.0062175907303645 7.795374536564983E-5 74-75 0.0010523755624362728 0.0 0.0 2.236980165449029 7.795374536564983E-5 76-77 0.0010523755624362728 0.0 0.0 2.502880390891261 7.795374536564983E-5 78-79 0.0010523755624362728 0.0 0.0 2.8030412874216957 7.795374536564983E-5 80-81 0.0011693061804847475 0.0 0.0 3.1386321612208183 7.795374536564983E-5 82-83 0.0011693061804847475 0.0 0.0 3.484922186571376 7.795374536564983E-5 84-85 0.0011693061804847475 0.0 0.0 3.8340185467550976 7.795374536564983E-5 86-87 0.0011693061804847475 0.0 0.0 4.21002843752631 7.795374536564983E-5 88-89 0.0011693061804847475 0.0 0.0 4.6241187329086415 7.795374536564983E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 44.716187 1 GTATCAA 8420 0.0 37.457607 1 ATCAACG 10160 0.0 30.94265 3 TCAACGC 10360 0.0 30.29953 4 CAACGCA 10365 0.0 30.284914 5 TATCAAC 10695 0.0 29.439129 2 AACGCAG 10800 0.0 29.091412 6 ACGCAGA 12155 0.0 25.614473 7 CGCAGAG 12285 0.0 25.381996 8 GTACATG 9320 0.0 23.44369 1 TACATGG 9150 0.0 23.16456 2 GCAGAGT 13625 0.0 22.607025 9 GTACCGT 210 3.5372068E-8 22.56606 6 ACATGGG 9340 0.0 21.576435 3 GAGTACT 7480 0.0 21.414457 12-13 GTCCTAC 620 0.0 20.685028 1 AGAGTAC 12160 0.0 19.621511 10-11 GTACTTT 8120 0.0 19.581285 14-15 CAGAGTA 13010 0.0 19.468702 10-11 AGTACTT 8020 0.0 18.613504 12-13 >>END_MODULE