FastQCFastQC Report
Fri 27 May 2016
SRR939320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565624
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.28129609015194745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61130.23826562271010873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.15762247312934397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.046.1164861
GTATCAA86800.038.426191
ATCAACG107250.030.882263
TCAACGC109700.030.365574
TATCAAC110000.030.2396162
CAACGCA110750.029.9919875
AACGCAG116450.028.6054366
ACGCAGA132000.025.1637337
CGCAGAG132800.024.8334878
TACATGG91750.023.273272
GTACATG94800.023.2381571
ACATGGG93600.021.9007423
GCAGAGT149550.021.5443959
GAGTACT81700.021.17068312-13
CGTATAG1604.9276663E-520.8164711
GTGGTAT30000.020.776821
TGGTATC28400.020.3841422
GTACTTT86350.020.0306314-15
CAGAGTA140650.019.39917810-11
AGAGTAC132350.019.16368110-11
Produced by FastQC (version 0.11.5)