##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2565624 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28990569155886 33.0 31.0 34.0 31.0 34.0 2 32.49484647789388 34.0 31.0 34.0 31.0 34.0 3 32.58308660972925 34.0 31.0 34.0 31.0 34.0 4 36.1182355637459 37.0 35.0 37.0 35.0 37.0 5 36.051718022594116 37.0 35.0 37.0 35.0 37.0 6 36.086334942298635 37.0 35.0 37.0 35.0 37.0 7 36.033787491853836 37.0 35.0 37.0 35.0 37.0 8 36.01058455954575 37.0 35.0 37.0 35.0 37.0 9 37.724797554123285 39.0 37.0 39.0 35.0 39.0 10-11 37.642055499948555 39.0 37.0 39.0 35.0 39.0 12-13 37.56367281409902 39.0 37.0 39.0 35.0 39.0 14-15 38.87773247365943 40.0 38.0 41.0 35.0 41.0 16-17 38.81902141545293 40.0 38.0 41.0 35.0 41.0 18-19 38.927749155760935 40.0 38.0 41.0 35.0 41.0 20-21 38.93018579495671 40.0 38.0 41.0 35.0 41.0 22-23 38.87223946299224 40.0 38.0 41.0 35.0 41.0 24-25 38.8135983682722 40.0 38.0 41.0 34.0 41.0 26-27 38.65123435858099 40.0 38.0 41.0 34.0 41.0 28-29 38.50437262046192 40.0 38.0 41.0 34.0 41.0 30-31 38.31547646888242 40.0 38.0 41.0 34.0 41.0 32-33 38.11775848682426 40.0 38.0 41.0 33.5 41.0 34-35 38.08039038456142 40.0 38.0 41.0 33.0 41.0 36-37 38.00068384923122 40.0 38.0 41.0 33.0 41.0 38-39 37.89968541766058 40.0 37.0 41.0 33.0 41.0 40-41 37.72733280480694 40.0 37.0 41.0 33.0 41.0 42-43 37.5542355388007 40.0 37.0 41.0 32.5 41.0 44-45 37.344070097566906 40.0 37.0 41.0 32.0 41.0 46-47 37.072693816397106 40.0 37.0 41.0 31.0 41.0 48-49 36.77246061776784 40.0 36.0 41.0 30.5 41.0 50-51 36.537245714882616 39.0 36.0 41.0 30.0 41.0 52-53 36.3080798277534 39.0 35.0 41.0 30.0 41.0 54-55 36.02659060719731 39.0 35.0 40.5 29.0 41.0 56-57 35.78936098976311 39.0 35.0 40.0 28.5 41.0 58-59 35.49808720997309 38.0 35.0 40.0 28.0 41.0 60-61 35.17289906860865 38.0 34.0 40.0 28.0 41.0 62-63 34.78860698216106 37.5 34.0 40.0 27.0 41.0 64-65 34.37650177890447 37.0 34.0 40.0 26.0 41.0 66-67 34.00751902850924 36.5 33.5 39.0 26.0 41.0 68-69 33.62175400604298 36.0 33.0 39.0 26.0 40.0 70-71 33.22847911463254 35.5 33.0 38.5 25.0 40.0 72-73 32.73396452480956 35.0 32.0 38.0 24.0 40.0 74-75 32.22627770086342 35.0 32.0 37.0 22.5 39.0 76-77 30.228502110987424 33.5 29.5 35.5 20.0 37.5 78-79 31.215639743002093 34.0 31.0 36.0 20.0 38.5 80-81 31.26165427981653 35.0 31.0 36.0 20.5 37.5 82-83 30.947781904129364 34.0 31.0 35.0 20.0 37.0 84-85 30.568907602984694 34.0 31.0 35.0 18.0 37.0 86-87 30.02417969273752 34.0 30.0 35.0 13.0 36.0 88-89 29.516351967396623 34.0 30.0 35.0 7.0 36.0 90-91 29.095846078770702 34.0 29.0 35.0 2.0 35.0 92-93 28.737163161866274 34.0 29.0 35.0 2.0 35.0 94-95 28.332477596093582 34.0 29.0 35.0 2.0 35.0 96-97 27.781336820983903 33.5 28.5 35.0 2.0 35.0 98-99 27.025354260795815 33.0 27.0 35.0 2.0 35.0 100-101 23.65263674646012 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27298151095389045 1101 2 0.19360785238675504 1101 3 0.2009348383900189 1101 4 0.08448069730651042 1101 5 0.16007355316060057 1101 6 0.13769652217754924 1101 7 0.18159868828182368 1101 8 0.219458773573443 1101 9 0.2544134304319172 1101 10-11 0.25350377815997405 1101 12-13 0.23116617356955516 1101 14-15 0.3660805130763336 1101 16-17 0.3482029995895033 1101 18-19 0.3298414542324153 1101 20-21 0.28380929947847733 1101 22-23 0.27251627592779926 1101 24-25 0.3335822689970982 1101 26-27 0.32531975939496505 1101 28-29 0.305113017028539 1101 30-31 0.26732081977959865 1101 32-33 0.13310799035034648 1101 34-35 0.2981077307772253 1101 36-37 0.5199018837780329 1101 38-39 0.46222646502292974 1101 40-41 0.4441914369008657 1101 42-43 0.5154343412651485 1101 44-45 0.5991037368647838 1101 46-47 0.5605429255295249 1101 48-49 0.5223495452934088 1101 50-51 0.5913164692606614 1101 52-53 0.6033779765308509 1101 54-55 0.528684827102083 1101 56-57 0.48325024179231235 1101 58-59 0.46828347722623676 1101 60-61 0.5332576885410489 1101 62-63 0.7724883195652197 1101 64-65 0.7147495186129547 1101 66-67 0.5377445490827313 1101 68-69 0.5200832301432214 1101 70-71 0.580602310946162 1101 72-73 0.3784299330278458 1101 74-75 0.3734082841252331 1101 76-77 0.7280600752790676 1101 78-79 0.8497490134597321 1101 80-81 0.8781978051179635 1101 82-83 0.845323151465756 1101 84-85 0.769503732559766 1101 86-87 0.8850230188123689 1101 88-89 0.9205667372685618 1101 90-91 0.9295136450999557 1101 92-93 0.7967227283257934 1101 94-95 0.9656340979688025 1101 96-97 1.022944494574162 1101 98-99 0.6702169956225816 1101 100-101 0.635318615916777 1102 1 0.13249671637689886 1102 2 0.12860143494348364 1102 3 0.1311067424457164 1102 4 0.025865794069837023 1102 5 0.07203479935649426 1102 6 0.07380110874708379 1102 7 0.09958787811695657 1102 8 0.17108472315494794 1102 9 0.224192542992796 1102 10-11 0.20856251030767226 1102 12-13 0.23368157458788374 1102 14-15 0.2676563237400629 1102 16-17 0.34013708790953956 1102 18-19 0.2363384320873081 1102 20-21 0.25492657719735945 1102 22-23 0.20770827218950672 1102 24-25 0.1691054808640402 1102 26-27 0.21081081358614284 1102 28-29 0.24786643557830246 1102 30-31 0.263125102697245 1102 32-33 0.30397911106648223 1102 34-35 0.43592211362886957 1102 36-37 0.4269227978226269 1102 38-39 0.4204423425159902 1102 40-41 0.5430448649767499 1102 42-43 0.5335469196230562 1102 44-45 0.610510270199697 1102 46-47 0.6035189394545597 1102 48-49 0.5761858911893682 1102 50-51 0.5423226578517628 1102 52-53 0.566578998361436 1102 54-55 0.5758283743445602 1102 56-57 0.7205955904282959 1102 58-59 0.6259116104858435 1102 60-61 0.5679705044317274 1102 62-63 0.49232760446418666 1102 64-65 0.620988615018895 1102 66-67 0.45437133572205113 1102 68-69 0.5558086887071738 1102 70-71 0.5019639700905856 1102 72-73 0.7504556298573419 1102 74-75 0.774824011004263 1102 76-77 0.7753011383481336 1102 78-79 0.8236302730034559 1102 80-81 0.8073731312464005 1102 82-83 0.8065941490752095 1102 84-85 0.9291571511658994 1102 86-87 0.8712910430346241 1102 88-89 0.7892901423633951 1102 90-91 0.958824478770083 1102 92-93 0.8089471545184317 1102 94-95 0.7722232931024813 1102 96-97 0.6980575537471054 1102 98-99 0.6242501205651543 1102 100-101 0.7330959878483405 1103 1 0.15312234329636 1103 2 0.16563411999219824 1103 3 0.16880546822083886 1103 4 0.0988682221062902 1103 5 0.1925450861512843 1103 6 0.12995164327693942 1103 7 0.11179184810016807 1103 8 0.033482499612205174 1103 9 0.11685535180833284 1103 10-11 0.2213591278428808 1103 12-13 0.17757860107321477 1103 14-15 0.2964214122389919 1103 16-17 0.2256598692627847 1103 18-19 0.2331477407665048 1103 20-21 0.16647763702463436 1103 22-23 0.2100948093260797 1103 24-25 0.1604843474346893 1103 26-27 0.22229968971748804 1103 28-29 0.19594948419834424 1103 30-31 0.29182183389396243 1103 32-33 0.2523315788490237 1103 34-35 0.4500885816583562 1103 36-37 0.37223397182618356 1103 38-39 0.25144872986707867 1103 40-41 0.2818393965417769 1103 42-43 0.18257572369294195 1103 44-45 0.32345361812959084 1103 46-47 0.3534807973855152 1103 48-49 0.2336811155039129 1103 50-51 0.2772171861714554 1103 52-53 0.2173780980220883 1103 54-55 0.22978244793618074 1103 56-57 0.26863118994142354 1103 58-59 0.17153325533599428 1103 60-61 0.23048024511415832 1103 62-63 0.1819217579284782 1103 64-65 0.2928602286279869 1103 66-67 0.1629847661320838 1103 68-69 0.04137531945128359 1103 70-71 0.04500631580731351 1103 72-73 0.25412817954181577 1103 74-75 0.2784294157261229 1103 76-77 0.2538928995866172 1103 78-79 0.28555879942891593 1103 80-81 0.3240240561496073 1103 82-83 0.3047399222811613 1103 84-85 0.24384339581155245 1103 86-87 0.10613838336670156 1103 88-89 0.3813113810611277 1103 90-91 0.3184276259290897 1103 92-93 0.31806572021250545 1103 94-95 0.332343680290176 1103 96-97 0.09537117704968878 1103 98-99 -0.025208784293042186 1103 100-101 0.03461053573705897 1104 1 0.10364743637351381 1104 2 0.14436038126908102 1104 3 0.1699399341884984 1104 4 0.1329715245576466 1104 5 0.1434717456102561 1104 6 0.11522687545581789 1104 7 0.1505158022353612 1104 8 0.20349355133038927 1104 9 0.17410048634125985 1104 10-11 0.18396769359768683 1104 12-13 0.2330735466497842 1104 14-15 0.21491853868308652 1104 16-17 0.18241670125883758 1104 18-19 0.10120816604477056 1104 20-21 0.12928935133644615 1104 22-23 0.19993565293935234 1104 24-25 0.13249142242661804 1104 26-27 0.18880410440036854 1104 28-29 0.21872914234108265 1104 30-31 0.09291028439357518 1104 32-33 0.14972345341620752 1104 34-35 0.17806438505495237 1104 36-37 0.18975835056277646 1104 38-39 0.29073527722952974 1104 40-41 0.2897333509935578 1104 42-43 0.39061385630555634 1104 44-45 0.2953230470101289 1104 46-47 0.24159334077634043 1104 48-49 0.24179432119723288 1104 50-51 0.2681902213619196 1104 52-53 0.26772063942236457 1104 54-55 0.23025279188588144 1104 56-57 0.35185650488991627 1104 58-59 0.3746721689197088 1104 60-61 0.3321594185023997 1104 62-63 0.38113370892245513 1104 64-65 0.31091752434496556 1104 66-67 0.2828254932965635 1104 68-69 0.27086732251243717 1104 70-71 0.19038953968485117 1104 72-73 0.36798007268986055 1104 74-75 0.2401740770038785 1104 76-77 0.4682822221057421 1104 78-79 0.5611265873640221 1104 80-81 0.38869889655108025 1104 82-83 0.4592479097585489 1104 84-85 0.44638005079816523 1104 86-87 0.5095715848824298 1104 88-89 0.8195898821759435 1104 90-91 0.8449050790775559 1104 92-93 0.709110498776031 1104 94-95 0.6092393483587166 1104 96-97 0.7084694281707797 1104 98-99 0.6750849577852556 1104 100-101 0.44366721318373337 1105 1 0.1323774566989897 1105 2 0.04739373797092128 1105 3 0.08466966604152759 1105 4 0.08285128945082931 1105 5 0.02139069756193379 1105 6 0.07973893771784901 1105 7 0.1356656499046025 1105 8 0.11264114700782102 1105 9 0.0822604963971898 1105 10-11 0.05643647581140243 1105 12-13 0.12463606281456663 1105 14-15 0.05292112999195098 1105 16-17 0.0475274056524313 1105 18-19 8.342287718150487E-4 1105 20-21 0.12011093112388238 1105 22-23 0.05801647430552492 1105 24-25 0.10893236793127414 1105 26-27 0.10432042683913068 1105 28-29 0.11813805628113272 1105 30-31 0.1436982078844693 1105 32-33 0.14021666186720694 1105 34-35 0.22727233044079753 1105 36-37 0.24986787123066279 1105 38-39 0.18592859173035237 1105 40-41 0.33002310162378024 1105 42-43 0.34711798328003596 1105 44-45 0.46089149398495977 1105 46-47 0.48504096440064615 1105 48-49 0.4291468520133819 1105 50-51 0.5107947604071228 1105 52-53 0.5064681393424735 1105 54-55 0.5529553091699526 1105 56-57 0.5396274443444256 1105 58-59 0.5838918474017945 1105 60-61 0.35206560669554676 1105 62-63 0.3304961124170376 1105 64-65 0.36605168963473744 1105 66-67 0.3580976486960026 1105 68-69 0.4632848046492981 1105 70-71 0.5885427863505157 1105 72-73 0.5159211579288794 1105 74-75 0.5443222897016398 1105 76-77 0.5800774793996872 1105 78-79 0.6359805989407015 1105 80-81 0.6551638502374786 1105 82-83 0.6555974621054403 1105 84-85 0.6533636264053975 1105 86-87 0.6449273972934151 1105 88-89 0.6464435604380618 1105 90-91 0.6921076289339467 1105 92-93 0.7158035363680142 1105 94-95 0.6720800005183172 1105 96-97 0.5057550330246059 1105 98-99 0.6764161857757678 1105 100-101 0.39895742547580326 1106 1 0.04776316269015979 1106 2 0.08324454611037879 1106 3 0.11630052792192203 1106 4 0.06817852854398154 1106 5 0.07366827354864824 1106 6 0.11002762646600672 1106 7 0.08839674943335751 1106 8 0.12990356616011667 1106 9 0.11589651632724696 1106 10-11 0.12635665769341387 1106 12-13 0.10599627899355824 1106 14-15 0.17311102926144883 1106 16-17 0.19995013617808866 1106 18-19 0.07326296802025212 1106 20-21 0.15947371957633294 1106 22-23 0.11713108225323765 1106 24-25 0.11307382946869637 1106 26-27 0.0894731429168516 1106 28-29 0.1755387435709963 1106 30-31 0.19273613710306847 1106 32-33 0.11744123956876962 1106 34-35 0.18555314371129583 1106 36-37 0.23709435619535668 1106 38-39 0.3373019055747264 1106 40-41 0.2768187307843135 1106 42-43 0.15817951867749258 1106 44-45 0.24878830598620993 1106 46-47 0.2501507923756421 1106 48-49 0.27020575437501293 1106 50-51 0.3309582449262223 1106 52-53 0.4486141816870912 1106 54-55 0.4001465688413006 1106 56-57 0.44735920213212665 1106 58-59 0.4841706791440572 1106 60-61 0.36776854286806326 1106 62-63 0.47325779566957493 1106 64-65 0.4718380390318444 1106 66-67 0.5905415447788585 1106 68-69 0.5261922500644332 1106 70-71 0.5467180250115788 1106 72-73 0.6062754700313917 1106 74-75 0.4581848013477625 1106 76-77 0.5403610033468098 1106 78-79 0.7898241561045829 1106 80-81 0.827548367986843 1106 82-83 0.8449584783222406 1106 84-85 0.8017727495369549 1106 86-87 0.8217196338885628 1106 88-89 1.0150541445669283 1106 90-91 1.0609644694259828 1106 92-93 0.9617174360966096 1106 94-95 1.022260599770263 1106 96-97 0.9597904135995492 1106 98-99 0.8776421508846681 1106 100-101 0.5682645826072736 1107 1 0.07763594778784721 1107 2 0.06274899214140106 1107 3 0.09797444237639041 1107 4 0.006209942836186144 1107 5 0.05448705721840952 1107 6 0.10961547614472522 1107 7 0.11871657159211679 1107 8 0.1460983153001223 1107 9 0.14556809418147054 1107 10-11 0.16843858473956885 1107 12-13 0.14909154413278003 1107 14-15 0.15526402789843274 1107 16-17 0.13972588745997427 1107 18-19 0.1386228693637932 1107 20-21 0.1745451968269336 1107 22-23 0.15414713015354664 1107 24-25 0.0719456085301644 1107 26-27 0.09238578002985065 1107 28-29 0.10753799715703138 1107 30-31 0.1136097659730595 1107 32-33 0.14247693762809632 1107 34-35 0.23387898586096867 1107 36-37 0.19048688881039766 1107 38-39 0.16132142592261545 1107 40-41 0.13350156975361216 1107 42-43 0.1725962610673193 1107 44-45 0.16203228599529496 1107 46-47 0.14177188992869816 1107 48-49 0.12296383511753106 1107 50-51 0.03278614782750111 1107 52-53 0.07040176200773374 1107 54-55 0.08475178075796208 1107 56-57 -0.049738302248989896 1107 58-59 0.01413433202910852 1107 60-61 -0.14945536509585367 1107 62-63 -0.15608844305307912 1107 64-65 -0.071363508965824 1107 66-67 -0.029826632023898014 1107 68-69 0.060072142321203614 1107 70-71 -0.17237719144846864 1107 72-73 -0.06868071871398485 1107 74-75 -0.02212082271921645 1107 76-77 -0.0014698203047771585 1107 78-79 0.19783999306180888 1107 80-81 0.15813024635382078 1107 82-83 0.19606917475892516 1107 84-85 0.17806234193002624 1107 86-87 0.08293653205173612 1107 88-89 0.19520561792319668 1107 90-91 0.20062217371624058 1107 92-93 0.10633433101450507 1107 94-95 0.2627470669605003 1107 96-97 0.3157921498233449 1107 98-99 0.4984821099941641 1107 100-101 0.3826448156182458 1108 1 -0.019748358047166903 1108 2 -0.005374969016848752 1108 3 0.05031675713450312 1108 4 0.02041254978221474 1108 5 0.01280872636371555 1108 6 0.06008022113641687 1108 7 0.04391668531794579 1108 8 0.14639575209480427 1108 9 0.13728251111787415 1108 10-11 0.1601737785108881 1108 12-13 0.0782638606405257 1108 14-15 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-0.9715391726584421 2316 100-101 -0.14000503923754692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 20.0 5 48.0 6 118.0 7 294.0 8 684.0 9 1291.0 10 2168.0 11 3297.0 12 4829.0 13 6991.0 14 8593.0 15 11281.0 16 14499.0 17 19710.0 18 18783.0 19 14002.0 20 12728.0 21 13585.0 22 14360.0 23 15959.0 24 18598.0 25 21637.0 26 26299.0 27 32462.0 28 39496.0 29 48009.0 30 58507.0 31 71881.0 32 88892.0 33 113842.0 34 154109.0 35 220344.0 36 349383.0 37 581269.0 38 526166.0 39 51467.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45844379227583 19.38261805183451 14.902587035393369 23.256351120496294 2 15.656970779818089 20.3223465324615 40.144346950293574 23.87633573742684 3 19.388655547344428 24.11432852202817 29.73171438994958 26.76530154067782 4 13.139883318833936 16.868644820909065 36.761076447679 33.230395412578 5 13.791693560708818 37.334387267970676 34.297309348524955 14.576609822795547 6 32.35770323320955 36.25963898061446 17.22594581279252 14.156711973383473 7 28.886189090841057 32.45218317259271 20.415345350682717 18.24628238588351 8 26.102616751324437 36.01583864198339 19.385030698184924 18.496513908507247 9 26.110490079606365 17.087772799131905 20.157825152867296 36.643911968394434 10-11 25.03652132970381 26.817140781345984 27.155382082487534 20.990955806462676 12-13 25.698777373457688 23.876783971462693 27.827460298157487 22.596978356922136 14-15 22.42212899576973 25.558543427411667 26.058766186056282 25.960561390762315 16-17 22.1184066541745 28.773789443630797 26.814078147183462 22.29372575501124 18-19 21.955691870671618 27.27726276336673 29.117282968977527 21.649762396984126 20-21 22.630323071502293 26.45674112808424 29.282291559480267 21.6306442409332 22-23 22.27746544310468 26.409520646828998 29.173000408477627 22.140013501588697 24-25 22.116655486795995 26.563801462646698 29.30356486514192 22.015978185415396 26-27 21.938601305273792 26.86114939553912 29.092193926392035 22.10805537279505 28-29 21.868286233680383 27.059206649142663 29.161424277290827 21.911082839886127 30-31 22.105791807373176 26.70968154335943 29.071095374848383 22.11343127441901 32-33 21.87421071832817 26.766256474058554 29.03215747903824 22.327375328575037 34-35 22.302569508555642 26.918458743117164 28.823094803626308 21.95587694470089 36-37 22.44733265706638 26.56850009863309 28.879165493855506 22.10500175044502 38-39 22.056064566048253 26.869692664207044 28.887576570834966 22.186666198909734 40-41 22.42182552360131 26.671100466699038 28.911228553100443 21.995845456599213 42-43 22.202732669207325 26.68675260000382 28.70898862082958 22.401526109959278 44-45 22.203025988971422 27.029504707949286 28.61821225577178 22.149257047307508 46-47 22.470349283857594 27.050507459987273 28.17144465219142 22.30769860396372 48-49 22.38176755440392 27.17457819228383 28.071513986460992 22.37214026685126 50-51 22.519570287774044 27.299265207996182 27.92147251506846 22.25969198916131 52-53 22.63110260895595 27.25613729837264 27.805146038546567 22.307614054124844 54-55 22.41513175742042 27.29271709338547 27.88598407249075 22.40616707670337 56-57 22.312767698138035 27.5218533666092 27.78321288825365 22.38216604699911 58-59 22.383637537203715 27.143491357200876 27.921670686512805 22.5512004190826 60-61 22.331316943269858 27.323816627061902 28.02187126116984 22.322995168498398 62-63 22.290653738605897 27.255628547440697 28.15107839079008 22.30263932316332 64-65 22.11951597730532 27.143051192039675 28.098939692437337 22.63849313821767 66-67 22.154220571681588 27.38630056469693 27.788931659510514 22.670547204110967 68-69 22.250060511901452 27.150374706064166 28.032835063460514 22.56672971857386 70-71 22.239701187645032 27.141277743986986 27.8808640472774 22.738157021090586 72-73 22.23396725319065 27.268629386067484 27.754534569367923 22.74286879137395 74-75 22.151959913065983 27.478402914846445 27.582217815237154 22.787419356850418 76-77 22.220082194012484 27.228451418939624 27.751252063005644 22.800214324042248 78-79 22.21510244681216 27.246003311475103 27.74993529839135 22.78895894332139 80-81 22.131708239219364 27.432486421278497 27.633453448420347 22.802351891081795 82-83 22.245621338122813 27.36215439206992 27.633141099397264 22.759083170410005 84-85 22.23402571849967 27.272916842062596 27.55216664639869 22.940890793039042 86-87 22.17795010607945 27.176960031214183 27.64333894213183 23.001750920574537 88-89 22.165397798672945 27.191562394566603 27.55382787402268 23.089211932737772 90-91 22.08195303116215 27.236637172191884 27.63822466719698 23.043185129448986 92-93 22.046995194931135 27.059070230088274 27.64590602520089 23.248028549779704 94-95 21.90904082379975 26.979016991386306 27.69630851915724 23.41563366565671 96-97 21.990478729541042 26.792351490319703 27.706144781932192 23.511024998207063 98-99 21.807774638840296 26.895308899511384 27.530105736460214 23.766810725188105 100-101 21.842065106667178 26.4637151129717 28.10988608798534 23.58433369237578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 419.0 1 287.0 2 229.5 3 340.0 4 310.5 5 289.0 6 553.0 7 1158.0 8 1829.0 9 2149.0 10 2007.0 11 1811.5 12 1888.0 13 2074.5 14 2264.5 15 2431.5 16 2646.5 17 2894.0 18 3150.5 19 3482.0 20 4001.0 21 4696.5 22 5662.0 23 7139.5 24 8916.0 25 11026.0 26 14330.0 27 18519.5 28 22140.5 29 26056.5 30 30667.5 31 36014.5 32 42963.5 33 47639.5 34 51027.0 35 56087.5 36 62405.5 37 68128.0 38 71965.5 39 74793.0 40 77819.5 41 81939.5 42 84913.5 43 87653.5 44 90603.5 45 93739.5 46 94302.5 47 94874.5 48 97105.0 49 95740.5 50 93177.5 51 91468.5 52 89780.5 53 88454.5 54 86670.0 55 79872.5 56 71152.5 57 64577.5 58 59101.5 59 52238.0 60 43383.0 61 36058.5 62 29554.0 63 21996.5 64 15782.5 65 11690.0 66 8454.5 67 6912.0 68 5399.0 69 3954.5 70 3056.5 71 2128.5 72 1595.0 73 1274.5 74 996.5 75 751.0 76 505.5 77 288.5 78 186.5 79 118.0 80 69.0 81 38.5 82 20.0 83 16.0 84 12.0 85 6.5 86 3.0 87 2.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2720975481988008 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0026114504697492694 16-17 6.431183992666111E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.8976872682824924E-5 26-27 0.0012472599258503976 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0036833144685269547 36-37 0.021905002447747606 38-39 0.007366628937053909 40-41 0.018728387324097373 42-43 0.005729600284375263 44-45 6.431183992666111E-4 46-47 1.1693061804847476E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.8976872682824924E-5 58-59 7.795374536564984E-4 60-61 0.002552985160725032 62-63 0.0016370286526786466 64-65 1.9488436341412462E-5 66-67 0.0 68-69 5.06699344876724E-4 70-71 1.9488436341412462E-5 72-73 0.0 74-75 0.0 76-77 0.23185392715378406 78-79 0.0 80-81 0.0011303293078019226 82-83 0.0 84-85 0.0 86-87 0.10471136846240915 88-89 0.3590354627178417 90-91 0.36129612133344563 92-93 0.0 94-95 1.9488436341412462E-5 96-97 0.0 98-99 0.0 100-101 1.3641905438988722E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2565624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.39112359191329 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96176311949326 41.52216285619579 2 14.245078197281085 15.781017740041323 3 4.745041164730026 7.884994848128313 4 2.0966374316862892 4.645404124238677 5 1.0737577773962987 2.9738324877768254 6 0.6675681046780807 2.2186408435345726 7 0.4160598676968412 1.6132216487261577 8 0.28474708720337627 1.261796887977562 9 0.21443570674708123 1.0690051261452167 >10 1.1641735180093342 11.971708282838614 >50 0.08881488567018786 3.381194689574811 >100 0.03840272093367314 3.6264791503097213 >500 0.0028163347795596228 1.0025336648612453 >1k 5.632669559064065E-4 0.5281378234436789 >5k 1.4081673897660163E-4 0.519869826207668 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7217 0.28129609015194745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6113 0.23826562271010873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4044 0.15762247312934397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.795374536564983E-5 0.0 4 0.0 0.0 0.0 7.795374536564983E-5 0.0 5 0.0 0.0 0.0 1.1693061804847475E-4 0.0 6 0.0 0.0 0.0 1.1693061804847475E-4 0.0 7 3.897687268282492E-5 0.0 0.0 1.1693061804847475E-4 0.0 8 3.897687268282492E-5 0.0 0.0 1.1693061804847475E-4 0.0 9 3.897687268282492E-5 0.0 0.0 1.1693061804847475E-4 0.0 10-11 3.897687268282492E-5 0.0 0.0 1.1693061804847475E-4 0.0 12-13 3.897687268282492E-5 0.0 0.0 1.5590749073129967E-4 3.897687268282492E-5 14-15 3.897687268282492E-5 0.0 0.0 1.5590749073129967E-4 3.897687268282492E-5 16-17 3.897687268282492E-5 0.0 0.0 1.5590749073129967E-4 3.897687268282492E-5 18-19 5.8465309024237376E-5 0.0 0.0 1.5590749073129967E-4 3.897687268282492E-5 20-21 7.795374536564983E-5 0.0 0.0 1.9488436341412457E-4 3.897687268282492E-5 22-23 7.795374536564983E-5 0.0 0.0 3.7028029048683675E-4 3.897687268282492E-5 24-25 1.5590749073129967E-4 0.0 0.0 5.261877812181364E-4 3.897687268282492E-5 26-27 1.5590749073129967E-4 0.0 0.0 7.990258899979108E-4 3.897687268282492E-5 28-29 1.7539592707271212E-4 0.0 0.0 0.0017344708343857089 3.897687268282492E-5 30-31 2.338612360969495E-4 0.0 0.0 0.005593181229985375 3.897687268282492E-5 32-33 2.338612360969495E-4 0.0 0.0 0.013037763912404935 7.795374536564983E-5 34-35 2.338612360969495E-4 0.0 0.0 0.026738134660417898 7.795374536564983E-5 36-37 2.338612360969495E-4 0.0 0.0 0.047785645909143346 7.795374536564983E-5 38-39 3.507918541454243E-4 0.0 0.0 0.0805457073990577 7.795374536564983E-5 40-41 3.507918541454243E-4 0.0 0.0 0.11718396772091312 7.795374536564983E-5 42-43 3.897687268282492E-4 0.0 0.0 0.15512795327764317 7.795374536564983E-5 44-45 3.897687268282492E-4 0.0 0.0 0.2027382032597138 7.795374536564983E-5 46-47 4.6772247219389906E-4 0.0 0.0 0.2644580811529671 1.3641905438988722E-4 48-49 5.06699344876724E-4 0.0 0.0 0.3296663891513332 2.338612360969495E-4 50-51 5.06699344876724E-4 0.0 0.0 0.404073239102846 2.338612360969495E-4 52-53 5.06699344876724E-4 0.0 0.0 0.4796688836711849 2.338612360969495E-4 54-55 5.06699344876724E-4 0.0 0.0 0.5644825586290119 2.338612360969495E-4 56-57 5.456762175595489E-4 0.0 0.0 0.6690769964733725 2.338612360969495E-4 58-59 5.456762175595489E-4 0.0 0.0 0.7861635220125787 2.338612360969495E-4 60-61 5.456762175595489E-4 0.0 0.0 0.916541161136628 2.338612360969495E-4 62-63 5.456762175595489E-4 0.0 0.0 1.0527263542904182 2.7283810877977443E-4 64-65 5.456762175595489E-4 0.0 0.0 1.20079949361247 2.7283810877977443E-4 66-67 5.846530902423738E-4 0.0 0.0 1.3714402422178775 2.7283810877977443E-4 68-69 5.846530902423738E-4 0.0 0.0 1.5696376398100425 2.7283810877977443E-4 70-71 6.041415265837862E-4 0.0 0.0 1.790655996358001 2.7283810877977443E-4 72-73 6.236299629251987E-4 0.0 0.0 2.016585438863996 2.7283810877977443E-4 74-75 6.236299629251987E-4 0.0 0.0 2.248653738817535 2.923265451211869E-4 76-77 6.236299629251987E-4 0.0 0.0 2.515216571095375 3.1181498146259934E-4 78-79 6.236299629251987E-4 0.0 0.0 2.81648830849727 3.1181498146259934E-4 80-81 6.236299629251987E-4 0.0 0.0 3.153774676258095 3.1181498146259934E-4 82-83 6.236299629251987E-4 0.0 0.0 3.501214519352797 3.1181498146259934E-4 84-85 6.431183992666111E-4 0.0 0.0 3.851636093207734 3.1181498146259934E-4 86-87 6.626068356080236E-4 0.0 0.0 4.228269613941871 3.1181498146259934E-4 88-89 6.626068356080236E-4 0.0 0.0 4.643646146122737 3.1181498146259934E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 46.116486 1 GTATCAA 8680 0.0 38.42619 1 ATCAACG 10725 0.0 30.88226 3 TCAACGC 10970 0.0 30.36557 4 TATCAAC 11000 0.0 30.239616 2 CAACGCA 11075 0.0 29.991987 5 AACGCAG 11645 0.0 28.605436 6 ACGCAGA 13200 0.0 25.163733 7 CGCAGAG 13280 0.0 24.833487 8 TACATGG 9175 0.0 23.27327 2 GTACATG 9480 0.0 23.238157 1 ACATGGG 9360 0.0 21.900742 3 GCAGAGT 14955 0.0 21.544395 9 GAGTACT 8170 0.0 21.170683 12-13 CGTATAG 160 4.9276663E-5 20.816471 1 GTGGTAT 3000 0.0 20.77682 1 TGGTATC 2840 0.0 20.384142 2 GTACTTT 8635 0.0 20.03063 14-15 CAGAGTA 14065 0.0 19.399178 10-11 AGAGTAC 13235 0.0 19.163681 10-11 >>END_MODULE