FastQCFastQC Report
Fri 27 May 2016
SRR939319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546684
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43610.7977186089221562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.5946762663622861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.4095601846770712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16940.30986822369046835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.1931646069758764No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9890.18090889801055088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.15840961140256526No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.13810537714657828No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6990.12786179950391816No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6020.1101184596585962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50550.030.7828641
GTATGGT1102.7564238E-630.1899011
TCAACGC59400.026.0176944
TATCAAC59700.025.8869512
ATCAACG59800.025.7643873
CAACGCA60050.025.7360735
AACGCAG60350.025.677276
GGTATCA33550.024.463011
GGTACTC1401.8097026E-523.6945468
ATCGATA1000.001676960823.6902039
GAGTACT34700.023.48323612-13
ACGCAGA67200.022.848317
AGTACTT35300.022.61435512-13
CGCAGAG67950.022.5961258
GTACTTT36800.022.02249314-15
TAGACAG1302.9781891E-421.879915
GCAGAGT68650.021.6714489
TAAGGTG1100.0029085221.5483975
CATTCCG1150.003775546620.6001789
GTGTAAG1856.6120792E-620.5151461
Produced by FastQC (version 0.11.5)