FastQCFastQC Report
Fri 27 May 2016
SRR939319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546684
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44800.8194862114128088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.6162609478236056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.4240109459943953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.3148070914824652No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT12460.22791960254918744No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11010.2013960532958711No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.19792055373854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.170848241397224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.1371907719999122No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT7000.1280447205332514RNA PCR Primer, Index 17 (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5960.1090209334825969No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5660.10353330260260041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA702.0250966E-434.004661
GGTATCA41100.029.7685551
CGCTTCT700.00842364827.1191277
TACGCTC1109.524179E-525.886445
CTACGCT1109.524179E-525.886444
CATTCCG1501.0665099E-625.3111869
GTATCAA55100.023.673661
GTGTAGC1652.3792236E-623.0819511
GATATAG4350.022.982461
GAGTACT35450.022.62684412-13
ACAGTCC1050.00220594222.5992748
CCCTATA1703.1679374E-622.3333992
GTACTTT36600.021.9158914-15
AGTATCG1100.00289008721.5720335
GACGTCT1553.9280043E-521.4328587
GGACGTC1553.9280043E-521.4328586
CTGACCA1353.814896E-421.0926559
CTTGGAC1150.003739718320.6341193
ATCAACG63650.020.3553243
CAATGCA1404.8759344E-420.3393469
Produced by FastQC (version 0.11.5)