##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939319_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 546684 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22552699548551 33.0 31.0 34.0 30.0 34.0 2 32.436116659715665 34.0 31.0 34.0 31.0 34.0 3 32.50423462182906 34.0 31.0 34.0 30.0 34.0 4 36.06014443444476 37.0 35.0 37.0 35.0 37.0 5 36.01016309238975 37.0 35.0 37.0 35.0 37.0 6 36.044431518025036 37.0 35.0 37.0 35.0 37.0 7 35.99579647474592 37.0 35.0 37.0 35.0 37.0 8 35.97857263062391 37.0 35.0 37.0 35.0 37.0 9 37.65206591010529 39.0 37.0 39.0 35.0 39.0 10-11 37.59673138412684 39.0 37.0 39.0 35.0 39.0 12-13 37.54594884796336 39.0 37.0 39.0 35.0 39.0 14-15 38.79547416789224 40.0 38.0 41.0 34.5 41.0 16-17 38.78933808196325 40.0 38.0 41.0 34.5 41.0 18-19 38.91749621353469 40.0 38.0 41.0 35.0 41.0 20-21 38.93240427742535 40.0 38.0 41.0 35.0 41.0 22-23 38.83582654696315 40.0 38.0 41.0 34.5 41.0 24-25 38.764528502754786 40.0 38.0 41.0 34.0 41.0 26-27 38.58347143871048 40.0 38.0 41.0 34.0 41.0 28-29 38.39404299375873 40.0 38.0 41.0 34.0 41.0 30-31 38.131452539309734 40.0 37.5 41.0 34.0 41.0 32-33 37.921176584644876 40.0 37.0 41.0 33.0 41.0 34-35 37.76119385239005 40.0 37.0 41.0 33.0 41.0 36-37 37.548643274725436 40.0 36.0 41.0 33.0 41.0 38-39 37.35535702526505 40.0 36.0 41.0 33.0 41.0 40-41 37.11429088833769 40.0 36.0 41.0 32.0 41.0 42-43 36.81990418596484 40.0 36.0 41.0 31.0 41.0 44-45 36.4195229785397 40.0 35.0 41.0 30.5 41.0 46-47 35.911538475609305 40.0 35.0 41.0 29.0 41.0 48-49 35.442104579610884 39.5 35.0 41.0 26.5 41.0 50-51 35.121608644116165 39.0 35.0 41.0 24.5 41.0 52-53 34.87523962654843 39.0 35.0 41.0 23.5 41.0 54-55 34.644401518976224 39.0 35.0 40.0 23.0 41.0 56-57 34.328257274769335 38.5 34.5 40.0 21.0 41.0 58-59 34.02391052234929 38.0 34.0 40.0 19.0 41.0 60-61 33.703480438425125 38.0 34.0 40.0 16.0 41.0 62-63 33.36327018899401 38.0 33.0 40.0 10.5 41.0 64-65 33.09390525422364 37.5 33.0 40.0 7.5 41.0 66-67 32.687689780567936 37.0 33.0 39.5 2.0 41.0 68-69 32.211502440166534 36.5 32.0 39.0 2.0 40.5 70-71 31.76874300327063 36.0 32.0 39.0 2.0 40.0 72-73 31.30392603405258 35.0 31.0 38.5 2.0 40.0 74-75 30.770901471416757 35.0 31.0 37.5 2.0 39.5 76-77 28.803062646794125 33.5 28.5 35.5 2.0 38.5 78-79 29.685762707523907 34.0 29.5 36.0 2.0 39.0 80-81 29.637856604546684 34.0 30.0 36.0 2.0 38.5 82-83 29.27374040579201 34.0 29.5 36.0 2.0 37.0 84-85 28.82202424069481 34.0 29.0 35.0 2.0 37.0 86-87 28.244317558223763 34.0 29.0 35.0 2.0 36.0 88-89 27.662250953018564 34.0 27.0 35.0 2.0 36.0 90-91 27.19767635416438 33.0 26.0 35.0 2.0 35.5 92-93 26.866705445924886 33.0 26.0 35.0 2.0 35.0 94-95 26.37976509281413 33.0 25.0 35.0 2.0 35.0 96-97 25.745692209759202 32.5 23.5 34.5 2.0 35.0 98-99 24.91165097204235 32.0 11.0 34.0 2.0 35.0 100-101 21.060037425642605 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2866594389453425 1101 2 0.40760618683754046 1101 3 0.36829885015443864 1101 4 0.2283105451469538 1101 5 0.3603041475615072 1101 6 0.3754303410389497 1101 7 0.3643037422124422 1101 8 0.36895036075722487 1101 9 0.4381486989996475 1101 10-11 0.5150814575810401 1101 12-13 0.3084843446175043 1101 14-15 0.5024984042553484 1101 16-17 0.5406603923307145 1101 18-19 0.48145459583161454 1101 20-21 0.5763076141457475 1101 22-23 0.5277506666911407 1101 24-25 0.47009077810948696 1101 26-27 0.5514161624829228 1101 28-29 0.40304165784683477 1101 30-31 0.5079600928021009 1101 32-33 0.7229367784371874 1101 34-35 0.6285934282092569 1101 36-37 0.6943997710001568 1101 38-39 0.7113434198972826 1101 40-41 0.8673029019431766 1101 42-43 0.848016287932694 1101 44-45 0.7856998854171451 1101 46-47 0.651835085308619 1101 48-49 0.3783298012710361 1101 50-51 0.21153037192014068 1101 52-53 0.17539620746567408 1101 54-55 0.40083380237622634 1101 56-57 0.20132108217698175 1101 58-59 0.22126143697921918 1101 60-61 0.1429856611645448 1101 62-63 0.09594311513892961 1101 64-65 0.15097598834363168 1101 66-67 -0.01561614760360186 1101 68-69 0.048485313231701355 1101 70-71 0.014921759806771462 1101 72-73 0.15619817119489454 1101 74-75 0.11537658638668091 1101 76-77 0.3571829516819527 1101 78-79 0.019739064101162995 1101 80-81 0.22051898139608284 1101 82-83 0.1826442968224491 1101 84-85 0.12918832354053578 1101 86-87 0.22413693020728687 1101 88-89 0.48062724415060387 1101 90-91 0.5317806043500539 1101 92-93 0.653162601540668 1101 94-95 0.3705695322305651 1101 96-97 0.28707973104193485 1101 98-99 -0.04316282526759707 1101 100-101 -0.2663196395574374 1102 1 0.14128721007251244 1102 2 0.08594841598039693 1102 3 0.1575401711146469 1102 4 0.07654773473120713 1102 5 0.09480248691038895 1102 6 0.0060952315439877225 1102 7 0.1261510703193025 1102 8 0.11495624729290199 1102 9 0.12457816408096534 1102 10-11 0.0443971253068014 1102 12-13 0.24045615405216125 1102 14-15 0.22257293103728415 1102 16-17 0.14030621010012823 1102 18-19 0.13088844431182878 1102 20-21 0.1595291873089053 1102 22-23 0.1776593083775353 1102 24-25 0.15961877515722023 1102 26-27 0.22138537968169203 1102 28-29 0.29559888055410255 1102 30-31 0.3321628452525758 1102 32-33 0.43397223266352114 1102 34-35 0.37388599983327 1102 36-37 0.4207501188818128 1102 38-39 0.4401622424101319 1102 40-41 0.4940456855364772 1102 42-43 0.2037601606251087 1102 44-45 0.34211701937633876 1102 46-47 0.25631209271629274 1102 48-49 0.266449620174555 1102 50-51 0.3094300307891018 1102 52-53 0.45301747100065626 1102 54-55 0.3848415970855399 1102 56-57 0.21677998893749617 1102 58-59 0.26338752045281666 1102 60-61 0.3159476507055956 1102 62-63 0.4497698133684622 1102 64-65 0.5796557482017803 1102 66-67 0.4870525952520026 1102 68-69 0.4628785230137922 1102 70-71 0.27682309282807793 1102 72-73 0.568026147540742 1102 74-75 0.6631484336247802 1102 76-77 0.7945462517422577 1102 78-79 0.70290123185843 1102 80-81 0.9189915423991373 1102 82-83 0.7809579933688369 1102 84-85 0.694401507940313 1102 86-87 0.798542201086935 1102 88-89 0.4862685075055708 1102 90-91 0.26086945143513773 1102 92-93 0.5391964806236622 1102 94-95 0.4829537591952189 1102 96-97 0.12270227269322831 1102 98-99 -0.049236127938140584 1102 100-101 0.3441271611014223 1103 1 0.26893904350835385 1103 2 0.2625914915441143 1103 3 0.2014291514419213 1103 4 0.16511399050265396 1103 5 0.2040766490219852 1103 6 0.24446787856968655 1103 7 0.2731204654110826 1103 8 0.24292859632572572 1103 9 0.2819166718643018 1103 10-11 0.2531610612383304 1103 12-13 0.29141380268043804 1103 14-15 0.26985764417582203 1103 16-17 0.35401431567970576 1103 18-19 0.3222427526478313 1103 20-21 0.46183603334880274 1103 22-23 0.5347699902094973 1103 24-25 0.5412553970960801 1103 26-27 0.42065295822966675 1103 28-29 0.3460107864091455 1103 30-31 0.3988322550707508 1103 32-33 0.46356145294436146 1103 34-35 0.4103966244921864 1103 36-37 0.47716302959656076 1103 38-39 0.4975778471884169 1103 40-41 0.4239805429070387 1103 42-43 0.6894452410572001 1103 44-45 0.6592931903410886 1103 46-47 0.6293619285603356 1103 48-49 0.6522464479364061 1103 50-51 0.5691581122866864 1103 52-53 0.43975072406071547 1103 54-55 0.6315974594890577 1103 56-57 0.592077765252256 1103 58-59 0.7164384824327357 1103 60-61 0.6703361156091319 1103 62-63 0.9990945649688783 1103 64-65 1.0632163299809037 1103 66-67 0.9330024042420249 1103 68-69 0.988069316419832 1103 70-71 1.0475548212733976 1103 72-73 0.9316251045656649 1103 74-75 0.7945165154688709 1103 76-77 1.2880969581759594 1103 78-79 1.2337876740486813 1103 80-81 1.4937019955388884 1103 82-83 1.1520219318991423 1103 84-85 1.258355197767841 1103 86-87 1.3293203609809048 1103 88-89 1.3026715428748972 1103 90-91 1.5654892073235303 1103 92-93 1.0697775667384057 1103 94-95 0.8810124741875534 1103 96-97 0.6303903157289419 1103 98-99 0.27056697153430775 1103 100-101 0.019070204613580444 1104 1 0.1697485531159586 1104 2 0.09146116049728903 1104 3 0.23428672035674936 1104 4 0.1558770598222381 1104 5 0.1133476643174518 1104 6 0.1540925217463709 1104 7 0.33826894651542716 1104 8 0.16230996056244607 1104 9 0.2525229519546244 1104 10-11 0.2549526338172612 1104 12-13 0.08346995708374294 1104 14-15 0.22482121624348395 1104 16-17 0.24001406004508397 1104 18-19 0.25961653305770227 1104 20-21 0.20684902810829442 1104 22-23 0.27429323165639374 1104 24-25 0.24435922735469973 1104 26-27 0.3254035014532235 1104 28-29 0.16044349059729512 1104 30-31 0.31823279190344067 1104 32-33 0.34493421081577935 1104 34-35 0.4304106843958948 1104 36-37 0.6685907489097573 1104 38-39 0.6438924481853903 1104 40-41 0.817167880472546 1104 42-43 0.8927770121790104 1104 44-45 0.7197230825484979 1104 46-47 0.5372461260806816 1104 48-49 0.5641547603218982 1104 50-51 0.5456354673205581 1104 52-53 0.4437237120032833 1104 54-55 0.8572440020309244 1104 56-57 0.9234867380271723 1104 58-59 1.0058424277269253 1104 60-61 0.8533513258686085 1104 62-63 0.8099654268835721 1104 64-65 0.6578842180129385 1104 66-67 0.9623045217487416 1104 68-69 1.2353063323117794 1104 70-71 1.1727795667039338 1104 72-73 1.0412838323574043 1104 74-75 1.0543712297971908 1104 76-77 0.7984331176919568 1104 78-79 0.7529986152101138 1104 80-81 0.9490660618334665 1104 82-83 1.1021028828599029 1104 84-85 1.0008161069749484 1104 86-87 0.8023232155674158 1104 88-89 1.0944614112230724 1104 90-91 0.9715477476498897 1104 92-93 1.1135592547789663 1104 94-95 0.8949530817458182 1104 96-97 0.9448379319361777 1104 98-99 0.980025452212594 1104 100-101 1.1380851592384396 1105 1 0.05673322650338264 1105 2 0.03053172848351693 1105 3 -0.010709591420969389 1105 4 0.14631227005091318 1105 5 0.13330504486003036 1105 6 -0.0018619082183803926 1105 7 0.02148343713094647 1105 8 -0.1031027105300879 1105 9 -0.057377875846377435 1105 10-11 -0.1557145246123781 1105 12-13 0.07610580660427502 1105 14-15 -0.0531232145408822 1105 16-17 0.04936985055405785 1105 18-19 0.1552931169442644 1105 20-21 0.014696745508501863 1105 22-23 -0.06792052131468296 1105 24-25 0.02316424333699274 1105 26-27 -0.009663296350240103 1105 28-29 0.19830514128165788 1105 30-31 0.10997117400327028 1105 32-33 0.014780944690052422 1105 34-35 0.23009923451473924 1105 36-37 0.08919151933841363 1105 38-39 0.26677991642569765 1105 40-41 0.19115361934640873 1105 42-43 0.42712665565085217 1105 44-45 0.20768718385166807 1105 46-47 -0.002090813514435297 1105 48-49 -0.04529528475637079 1105 50-51 -0.19863959210080395 1105 52-53 -0.06527145232751508 1105 54-55 -0.4154508590460395 1105 56-57 -0.06018263333583462 1105 58-59 -0.20627952948969863 1105 60-61 -0.37651098049972376 1105 62-63 -0.1968331961801013 1105 64-65 -0.3617822027722397 1105 66-67 -0.06164843292710742 1105 68-69 -0.1867615309781101 1105 70-71 0.0389462743508453 1105 72-73 0.20315171203939997 1105 74-75 0.14587602448614945 1105 76-77 0.14858064658303505 1105 78-79 0.2653418657633466 1105 80-81 0.14182895012130814 1105 82-83 0.20208649076089458 1105 84-85 0.24116116065907178 1105 86-87 0.3330911854059586 1105 88-89 0.5766882778733446 1105 90-91 0.841119913865775 1105 92-93 0.5660549518529798 1105 94-95 0.6762455987526188 1105 96-97 0.39216601979782695 1105 98-99 0.469190274768124 1105 100-101 0.9848477196843 1106 1 -0.006169814230986503 1106 2 0.049556398592528694 1106 3 0.13088535981253102 1106 4 -0.0016739605859257267 1106 5 -0.12787682547846657 1106 6 -0.031213600702606925 1106 7 -0.00662901266824889 1106 8 -0.0036764339953805347 1106 9 -0.03713691199095592 1106 10-11 0.06699345014379077 1106 12-13 0.10231349449870919 1106 14-15 0.014685161821716974 1106 16-17 0.12967392399066568 1106 18-19 -0.07725421524162357 1106 20-21 -0.032606754204628885 1106 22-23 0.10184425206455217 1106 24-25 0.15640004368122362 1106 26-27 0.0211858143783914 1106 28-29 0.013164579032668655 1106 30-31 0.12918517285581999 1106 32-33 0.05717910877496024 1106 34-35 0.26149911977004336 1106 36-37 0.19920299380771667 1106 38-39 0.07661353662076209 1106 40-41 0.22968488727526903 1106 42-43 -0.07633863407949093 1106 44-45 0.09870267438522973 1106 46-47 -0.0596926494295289 1106 48-49 -0.15149149818149965 1106 50-51 -0.36429650546855896 1106 52-53 -0.11729669615998262 1106 54-55 -0.1443206238194179 1106 56-57 0.07518741829927222 1106 58-59 -0.02708274234110064 1106 60-61 -0.15379153127425127 1106 62-63 0.06853685545500099 1106 64-65 -0.20885047586460814 1106 66-67 -0.10388595444173632 1106 68-69 -0.14210863367461002 1106 70-71 -0.10048573941851302 1106 72-73 -0.1551787526766084 1106 74-75 -0.02147911033886274 1106 76-77 0.04618821973277676 1106 78-79 0.08660405738698174 1106 80-81 0.15688919108516686 1106 82-83 0.42108247469663596 1106 84-85 0.40408141309739776 1106 86-87 0.48334362373068274 1106 88-89 0.6072505268693291 1106 90-91 0.44134366601723585 1106 92-93 0.379273540493255 1106 94-95 0.3491027416097623 1106 96-97 0.0942256870382181 1106 98-99 0.15934518010374887 1106 100-101 0.607745023184016 1107 1 0.23076749306603261 1107 2 0.18349610397108052 1107 3 0.21707926183377424 1107 4 0.18906601885909424 1107 5 0.27745719302102145 1107 6 0.21391486795103987 1107 7 0.1138745844540523 1107 8 0.07832082534000762 1107 9 0.25166404724166114 1107 10-11 0.2228464820040159 1107 12-13 0.24870266887349857 1107 14-15 0.26720486034860613 1107 16-17 0.36926453721437724 1107 18-19 0.3531637361115827 1107 20-21 0.3456392204442267 1107 22-23 0.4575482602865435 1107 24-25 0.4354031269289109 1107 26-27 0.4490600863893519 1107 28-29 0.4854067852676778 1107 30-31 0.3485513941644882 1107 32-33 0.4646559844172984 1107 34-35 0.6147999077111237 1107 36-37 0.7480210821941498 1107 38-39 0.668187705856802 1107 40-41 0.7830319239316594 1107 42-43 0.5337401600280742 1107 44-45 0.5761538562968411 1107 46-47 0.5637914204574273 1107 48-49 0.6124002455663629 1107 50-51 0.4654122989165046 1107 52-53 0.4354833655049717 1107 54-55 0.4633737920764389 1107 56-57 0.44771225557231986 1107 58-59 0.5224736125749629 1107 60-61 0.7786853443680926 1107 62-63 0.7909777598743304 1107 64-65 0.7317610349267554 1107 66-67 0.8529776974938628 1107 68-69 0.9539379576237721 1107 70-71 0.7373405709617522 1107 72-73 0.8895917166622063 1107 74-75 0.6116058502640804 1107 76-77 0.5703865753340054 1107 78-79 0.7524810700625579 1107 80-81 0.8730162894065074 1107 82-83 0.7919098128261339 1107 84-85 0.7855870657181576 1107 86-87 1.1852552372969747 1107 88-89 1.2745047235120168 1107 90-91 1.2242676125742769 1107 92-93 1.229258124254816 1107 94-95 1.4409338481531009 1107 96-97 1.3858855020433545 1107 98-99 0.9557155124093342 1107 100-101 1.2065185757705201 1108 1 0.05791698504568643 1108 2 0.04589154024412068 1108 3 0.01672020007775643 1108 4 0.10183417684626761 1108 5 0.09823529025390343 1108 6 -0.0016295139033317696 1108 7 -0.11693642677312965 1108 8 0.0822789608207728 1108 9 0.05409877215612369 1108 10-11 0.13972401072244622 1108 12-13 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-0.9118540182058439 2316 46-47 -0.7304253551192517 2316 48-49 -0.7003999440813544 2316 50-51 -0.5795414229197746 2316 52-53 -0.5282954681616232 2316 54-55 -0.5021443378322488 2316 56-57 -0.3889072740189121 2316 58-59 -0.2256118731911485 2316 60-61 -0.36549844899835904 2316 62-63 -0.5804766525269898 2316 64-65 -0.9397387280136087 2316 66-67 -0.6546623222954722 2316 68-69 -0.718754833701901 2316 70-71 -0.8256064925908468 2316 72-73 -0.7454420901032677 2316 74-75 -0.8463392549697808 2316 76-77 -0.6675981085521201 2316 78-79 -0.6061412011748963 2316 80-81 -0.909968497549027 2316 82-83 -1.058946178598493 2316 84-85 -0.9934707768330178 2316 86-87 -0.818903613273001 2316 88-89 -1.1764935036941822 2316 90-91 -1.4350132721735136 2316 92-93 -1.1165062384670428 2316 94-95 -1.1141077918873066 2316 96-97 -1.2793682548365268 2316 98-99 -1.4809972949951735 2316 100-101 -0.8799927309434246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 8.0 5 29.0 6 66.0 7 132.0 8 279.0 9 559.0 10 946.0 11 1435.0 12 2092.0 13 2948.0 14 3538.0 15 4838.0 16 6600.0 17 9180.0 18 8572.0 19 4931.0 20 4045.0 21 3939.0 22 4244.0 23 4469.0 24 4875.0 25 5464.0 26 5989.0 27 6902.0 28 8335.0 29 9931.0 30 11950.0 31 14364.0 32 17338.0 33 22110.0 34 29531.0 35 42843.0 36 63999.0 37 115316.0 38 115129.0 39 9754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.17234901643524 23.773227005974952 16.269590118673513 20.78483385891629 2 14.690204944721266 23.78577020728611 40.76980485984591 20.754219988146716 3 17.146834368666358 28.799635621309566 32.822800740464324 21.230729269559745 4 11.441344542734011 20.415267320792267 40.45097350571811 27.692414630755614 5 10.714416372163809 40.18445756597961 36.33817708219008 12.7629489796665 6 25.614065895471605 41.47295329660279 18.833549180147948 14.079431627777655 7 23.422489043030343 36.37640757732072 23.808086572864763 16.393016806784175 8 22.83805635431072 36.452868567582 22.369229756129684 18.339845321977595 9 24.580379158709604 18.17759436895903 24.029603939387286 33.212422532944075 10-11 21.744188598898084 29.20105582018131 29.75850765707429 19.296247923846316 12-13 23.24377519737179 27.998167131286085 29.607231965815718 19.15082570552641 14-15 20.833725855324474 29.6445717181122 28.75565803301209 20.766044393551237 16-17 18.00899795949912 32.38442268276019 30.104659128428995 19.50192022931169 18-19 17.62252050544739 30.66561304153771 33.788257933285045 17.92360851972986 20-21 18.658127913017392 29.575495167226407 33.73978386051174 18.026593059244465 22-23 18.66809710911605 29.65973030123435 33.30717562613869 18.36499696351091 24-25 18.446579742593528 30.10258211325007 33.651067161285134 17.799770982871273 26-27 18.167275946685788 29.993314230263028 34.12897956495852 17.710430258092664 28-29 17.701908232178003 29.93466060832218 34.32010082607137 18.043330333428454 30-31 18.514809286534817 29.777257062580944 33.78633726247705 17.921596388407195 32-33 17.830410255284587 30.52586137512713 33.66249972561845 17.98122864396983 34-35 18.23158183564275 30.04774317464673 33.794576302190514 17.926098687520007 36-37 18.426367338277423 29.685664945869906 33.35970628157894 18.528261434273734 38-39 17.753601317053093 30.262587460557004 33.543878904284995 18.43993231810491 40-41 17.820338688508098 29.983125025724043 34.03540543383318 18.161130851934676 42-43 18.06147255356395 29.86822344942133 33.1924533962095 18.87785060080522 44-45 18.09542885731036 30.007984568654944 32.91005058722707 18.986535986807628 46-47 18.6413162277625 30.0600804670344 32.23327574364326 19.06532756155984 48-49 18.51989043000279 30.101750101750103 32.094771645333445 19.283587822913663 50-51 18.124730191481735 30.384280498423223 31.8151802503823 19.67580905971274 52-53 18.192776814393692 30.86389943733491 31.850209627499616 19.09311412077178 54-55 18.580020633492108 30.294832115079277 31.349737691243934 19.775409560184677 56-57 18.599878173925926 30.754041205017185 30.856294621543462 19.789785999513427 58-59 18.24104383488224 30.262047115615907 31.530804781255462 19.966104268246397 60-61 18.49987744246198 30.81123944084495 30.93288237037254 19.756000746320527 62-63 18.091329646831138 30.513793286370976 31.50688063744903 19.88799642934885 64-65 18.152534187940383 30.372482092031227 31.22013814196135 20.254845578067037 66-67 18.060574465068846 30.517896585312315 30.65783155670796 20.763697392910878 68-69 17.965188012354588 30.151578758669384 30.924700853059246 20.958532375916786 70-71 18.25588456951365 30.088680115020743 30.907343181801554 20.748092133664052 72-73 18.361795845497582 29.508911912549113 30.77445105399097 21.354841187962332 74-75 17.910255284588537 30.358031330713903 30.054839724594096 21.67687366010346 76-77 17.956558694541915 29.93074587780723 30.6010062157248 21.51168921192605 78-79 17.794665469142565 29.751492408313034 30.601801590865644 21.852040531678753 80-81 18.008758258884473 29.72256367481031 30.11154524368739 22.157132822617818 82-83 18.01534341594047 29.7447885798743 30.021273715711455 22.21859428847378 84-85 17.710871362615332 29.28355320441059 30.11822186125806 22.88735357171602 86-87 17.62838119932321 29.64578435034828 29.942703641038037 22.78313080929047 88-89 18.05997440788399 29.250742153005604 29.789021092383134 22.900262346727274 90-91 17.858302290647575 28.84346966437217 29.889016666758373 23.409211378221883 92-93 18.10671247009241 29.133649420872022 29.666955681893015 23.092682427142556 94-95 17.93376063685786 28.55635065229639 29.769665839863613 23.740222870982137 96-97 17.969338777063165 28.50467546150976 29.383611007455862 24.142374753971215 98-99 17.84688947078154 28.758779074180946 29.1574558222445 24.236875632793016 100-101 17.778310282192788 28.418114336827742 29.757373102876805 24.046202278102665 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1293.0 1 813.5 2 353.5 3 356.0 4 290.5 5 292.5 6 537.0 7 919.5 8 1259.0 9 1347.0 10 1124.0 11 971.0 12 977.0 13 996.0 14 1084.5 15 1210.0 16 1315.0 17 1403.0 18 1539.5 19 1665.5 20 1882.5 21 2208.0 22 2515.0 23 2919.0 24 3486.5 25 4268.5 26 5675.5 27 7639.5 28 8730.5 29 9770.5 30 11848.0 31 13782.0 32 16250.5 33 19536.0 34 22556.0 35 24836.0 36 27079.0 37 28778.0 38 27707.5 39 25362.0 40 23536.5 41 22038.5 42 20387.0 43 19521.0 44 19237.5 45 18413.0 46 16881.0 47 15784.5 48 16696.5 49 15276.0 50 11761.0 51 9139.0 52 7009.0 53 5715.5 54 4827.0 55 4125.0 56 3584.5 57 3155.5 58 2824.5 59 2586.5 60 2296.5 61 2011.5 62 1833.0 63 1702.0 64 1555.5 65 1400.0 66 1263.5 67 1135.5 68 1004.5 69 867.5 70 749.0 71 617.0 72 470.0 73 388.0 74 300.0 75 224.5 76 157.5 77 97.0 78 68.0 79 47.0 80 26.0 81 13.5 82 11.0 83 7.0 84 3.5 85 3.5 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2881006211998156 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010060656613326893 16-17 8.231446319994732E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.146051466660813E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016462892639989464 36-37 0.0069509991146622175 38-39 0.0016462892639989464 40-41 0.0030181969839980687 42-43 1.8292102933321625E-4 44-45 8.231446319994732E-4 46-47 9.146051466660813E-5 48-49 2.7438154399982444E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.487630879996489E-4 58-59 0.001371907719999122 60-61 3.658420586664325E-4 62-63 9.146051466660813E-4 64-65 0.0 66-67 2.7438154399982444E-4 68-69 8.231446319994732E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.25124203378917254 78-79 9.146051466660813E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.10536251289593257 88-89 0.3630982432264343 90-91 0.2683451500318283 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.146051466660813E-5 100-101 1.8292102933321625E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 546684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.3809308115367 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03520250000902 33.418587897056106 2 11.61962622130193 10.081404023244334 3 4.159096735440008 5.4127646315583355 4 1.9794180241155444 3.434759854050605 5 1.0856287143710668 2.3547792072574394 6 0.6852356432537824 1.7835696017754705 7 0.4910783815577601 1.491240610537903 8 0.34103172691659606 1.1835418999926186 9 0.27793291119288943 1.085128955163694 >10 1.9077015941415965 16.75955178982248 >50 0.2594341491850864 7.844657428274148 >100 0.15312942462990825 11.603475716223901 >500 0.002531064869915839 0.743528661425674 >1k 0.002952909014901812 2.80300972361729 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4480 0.8194862114128088 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3369 0.6162609478236056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2318 0.4240109459943953 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1721 0.3148070914824652 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1246 0.22791960254918744 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1101 0.2013960532958711 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.19792055373854 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.170848241397224 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.1371907719999122 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 700 0.1280447205332514 RNA PCR Primer, Index 17 (95% over 21bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 596 0.1090209334825969 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 566 0.10353330260260041 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.8292102933321625E-4 3 0.0 0.0 0.0 0.0 1.8292102933321625E-4 4 0.0 0.0 0.0 0.0 1.8292102933321625E-4 5 0.0 0.0 0.0 0.0 1.8292102933321625E-4 6 0.0 0.0 0.0 0.0 1.8292102933321625E-4 7 0.0 0.0 0.0 0.0 1.8292102933321625E-4 8 0.0 0.0 0.0 0.0 1.8292102933321625E-4 9 0.0 0.0 0.0 0.0 1.8292102933321625E-4 10-11 0.0 0.0 0.0 0.0 1.8292102933321625E-4 12-13 0.0 0.0 0.0 1.8292102933321625E-4 1.8292102933321625E-4 14-15 0.0 0.0 0.0 1.8292102933321625E-4 1.8292102933321625E-4 16-17 0.0 0.0 0.0 1.8292102933321625E-4 1.8292102933321625E-4 18-19 0.0 0.0 0.0 8.231446319994732E-4 1.8292102933321625E-4 20-21 0.0 0.0 0.0 0.0010060656613326893 1.8292102933321625E-4 22-23 0.0 0.0 0.0 0.0022865128666652032 1.8292102933321625E-4 24-25 0.0 0.0 0.0 0.006310775511995961 1.8292102933321625E-4 26-27 0.0 0.0 0.0 0.014999524405323733 1.8292102933321625E-4 28-29 0.0 0.0 0.0 0.03548667969064395 1.8292102933321625E-4 30-31 0.0 0.0 0.0 0.08295468680261357 1.8292102933321625E-4 32-33 0.0 0.0 0.0 0.17624441176255387 1.8292102933321625E-4 34-35 0.0 0.0 0.0 0.33264189184245374 1.8292102933321625E-4 36-37 0.0 0.0 0.0 0.5528788111596461 1.8292102933321625E-4 38-39 0.0 0.0 0.0 0.846284142210125 1.8292102933321625E-4 40-41 0.0 0.0 0.0 1.1367627367912725 1.8292102933321625E-4 42-43 0.0 0.0 0.0 1.4264181867404204 1.8292102933321625E-4 44-45 0.0 0.0 0.0 1.7913456402601868 1.8292102933321625E-4 46-47 0.0 0.0 0.0 2.211149402579918 1.8292102933321625E-4 48-49 0.0 0.0 0.0 2.63040440181165 1.8292102933321625E-4 50-51 0.0 0.0 0.0 3.0470985066327163 1.8292102933321625E-4 52-53 0.0 0.0 0.0 3.4579391385151204 1.8292102933321625E-4 54-55 0.0 0.0 0.0 3.923656079197489 1.8292102933321625E-4 56-57 0.0 0.0 0.0 4.478181179621133 1.8292102933321625E-4 58-59 0.0 0.0 0.0 5.077064629658084 1.8292102933321625E-4 60-61 0.0 0.0 0.0 5.6681739359483725 1.8292102933321625E-4 62-63 0.0 0.0 0.0 6.260289307899994 1.8292102933321625E-4 64-65 0.0 0.0 0.0 6.91103086975291 1.8292102933321625E-4 66-67 0.0 0.0 0.0 7.648934302083104 1.8292102933321625E-4 68-69 0.0 0.0 0.0 8.44427493762393 1.8292102933321625E-4 70-71 0.0 0.0 0.0 9.304186696519379 1.8292102933321625E-4 72-73 0.0 0.0 0.0 10.128245933665518 1.8292102933321625E-4 74-75 0.0 0.0 0.0 11.026754029750276 1.8292102933321625E-4 76-77 0.0 0.0 0.0 11.986632131176329 1.8292102933321625E-4 78-79 0.0 0.0 0.0 13.051872745498313 1.8292102933321625E-4 80-81 0.0 0.0 0.0 14.186348969422921 1.8292102933321625E-4 82-83 0.0 0.0 0.0 15.294210183579544 1.8292102933321625E-4 84-85 0.0 0.0 0.0 16.4107601466295 1.8292102933321625E-4 86-87 0.0 0.0 0.0 17.56993070951409 1.8292102933321625E-4 88-89 0.0 0.0 0.0 18.79275779060664 1.8292102933321625E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGA 70 2.0250966E-4 34.00466 1 GGTATCA 4110 0.0 29.768555 1 CGCTTCT 70 0.008423648 27.119127 7 TACGCTC 110 9.524179E-5 25.88644 5 CTACGCT 110 9.524179E-5 25.88644 4 CATTCCG 150 1.0665099E-6 25.311186 9 GTATCAA 5510 0.0 23.67366 1 GTGTAGC 165 2.3792236E-6 23.081951 1 GATATAG 435 0.0 22.98246 1 GAGTACT 3545 0.0 22.626844 12-13 ACAGTCC 105 0.002205942 22.599274 8 CCCTATA 170 3.1679374E-6 22.333399 2 GTACTTT 3660 0.0 21.91589 14-15 AGTATCG 110 0.002890087 21.572033 5 GACGTCT 155 3.9280043E-5 21.432858 7 GGACGTC 155 3.9280043E-5 21.432858 6 CTGACCA 135 3.814896E-4 21.092655 9 CTTGGAC 115 0.0037397183 20.634119 3 ATCAACG 6365 0.0 20.355324 3 CAATGCA 140 4.8759344E-4 20.339346 9 >>END_MODULE