FastQCFastQC Report
Fri 27 May 2016
SRR939318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557298
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46150.8281027385707468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.5960904220004378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.4166532088756823No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16430.2948153411639733No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.19020344591224084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10530.18894738542036757No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.146061891483551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.1428320216473054No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6950.12470886312170508No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG250.005009530657.0298231
CCCTATA1703.6925485E-927.8980142
CGGAAAC350.00834069427.17663634-35
CGGGGAT700.00845140127.100934
GTACAGG1257.351973E-626.6139181
ACTCCCG950.001245954424.930026
GGTATCA38400.024.7525271
GTATCAA51600.024.1308361
CCTATAC2950.024.1152343
GTACATG25400.022.6397911
GAGTACT34100.022.5763212-13
ACACAGT2154.5440174E-822.0311786
CATTCCG1553.9870283E-521.3915659
GTACTTT36150.021.23148314-15
TACATGG26850.021.1962572
AGTACTT35250.020.89899612-13
GTATAGG3201.8189894E-1120.7921261
GTGGTAC1150.003709630620.662981
CATGGGG9200.020.6202724
CAACGCA60450.020.555465
Produced by FastQC (version 0.11.5)