##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939318_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557298 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82654163481656 33.0 31.0 34.0 30.0 34.0 2 31.908618010471955 33.0 31.0 34.0 30.0 34.0 3 31.934406726742246 34.0 31.0 34.0 30.0 34.0 4 35.457537977886155 37.0 35.0 37.0 33.0 37.0 5 35.50394761868874 37.0 35.0 37.0 33.0 37.0 6 35.61872283769186 37.0 35.0 37.0 33.0 37.0 7 35.55369479165545 37.0 35.0 37.0 33.0 37.0 8 35.54511589849596 37.0 35.0 37.0 33.0 37.0 9 37.173749053468704 39.0 37.0 39.0 34.0 39.0 10-11 37.095706426364345 39.0 37.0 39.0 33.5 39.0 12-13 37.027412623049074 39.0 37.0 39.0 33.0 39.0 14-15 38.22698358867249 40.0 38.0 41.0 33.0 41.0 16-17 38.17755491675908 40.0 38.0 41.0 33.0 41.0 18-19 38.19438881890838 40.0 38.0 41.0 33.5 41.0 20-21 38.135295658696066 40.0 38.0 41.0 33.5 41.0 22-23 38.042869344587636 40.0 38.0 41.0 33.0 41.0 24-25 38.037832542015224 40.0 38.0 41.0 33.0 41.0 26-27 37.935346080552954 40.0 38.0 41.0 33.0 41.0 28-29 37.78462330745849 40.0 38.0 41.0 33.0 41.0 30-31 37.552264677066844 40.0 37.0 41.0 33.0 41.0 32-33 37.27542445872764 40.0 36.5 41.0 32.5 41.0 34-35 36.94562424412074 40.0 36.0 41.0 31.0 41.0 36-37 36.67358307404656 40.0 35.0 41.0 30.5 41.0 38-39 36.342376071688754 39.0 35.0 41.0 30.0 41.0 40-41 36.16950177463404 39.0 35.0 41.0 30.0 41.0 42-43 35.90424243402991 39.0 35.0 41.0 29.5 41.0 44-45 35.639189446220875 39.0 35.0 41.0 27.5 41.0 46-47 35.263486500938455 39.0 35.0 41.0 24.5 41.0 48-49 34.93420755143568 39.0 35.0 41.0 22.5 41.0 50-51 33.76669573549519 38.0 34.0 40.0 17.5 40.5 52-53 33.87150770323956 38.5 34.5 39.5 18.0 40.5 54-55 34.37203614583221 39.0 35.0 40.5 16.0 41.0 56-57 34.292587628163034 39.0 35.0 41.0 13.5 41.0 58-59 34.13231789814426 39.0 35.0 41.0 9.0 41.0 60-61 33.869994150346855 39.0 34.0 41.0 7.0 41.0 62-63 33.53368843957811 38.0 34.0 40.0 2.0 41.0 64-65 33.204757598268785 38.0 34.0 40.0 2.0 41.0 66-67 32.85894620113476 37.5 33.5 40.0 2.0 41.0 68-69 32.446455038417504 37.0 33.0 40.0 2.0 41.0 70-71 32.04822285384121 36.5 32.5 39.0 2.0 41.0 72-73 31.593216735032243 36.0 32.0 39.0 2.0 40.5 74-75 31.11795305204756 35.0 31.5 38.5 2.0 40.0 76-77 30.640436534852093 35.0 31.0 37.5 2.0 39.5 78-79 30.169352841747145 35.0 31.0 37.0 2.0 39.0 80-81 29.65109959124203 35.0 30.0 36.5 2.0 39.0 82-83 29.15739245430631 35.0 29.5 36.0 2.0 37.5 84-85 28.664176975334563 34.0 29.0 35.5 2.0 37.0 86-87 28.281159272059114 34.0 29.0 35.0 2.0 37.0 88-89 27.815017279803623 34.0 28.0 35.0 2.0 36.0 90-91 27.353696765464797 34.0 26.5 35.0 2.0 36.0 92-93 26.896118414205688 34.0 25.5 35.0 2.0 35.5 94-95 26.401917824933875 34.0 25.0 35.0 2.0 35.0 96-97 25.838365649975415 34.0 22.0 35.0 2.0 35.0 98-99 25.161725863003277 33.0 10.0 35.0 2.0 35.0 100-101 22.89717350501886 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2796984542943868 1101 2 0.1657941767408886 1101 3 0.18765802504674056 1101 4 0.17934490996833574 1101 5 0.32658152916525296 1101 6 0.2970166567365311 1101 7 0.17013090531199992 1101 8 0.12692863187727 1101 9 0.23810355406993722 1101 10-11 0.2664852147521799 1101 12-13 0.3583196270887328 1101 14-15 0.34969867065044724 1101 16-17 0.6003076199469675 1101 18-19 0.48437492613532385 1101 20-21 0.5976840432783987 1101 22-23 0.6568155191598564 1101 24-25 0.5221920094360897 1101 26-27 0.5755611689650593 1101 28-29 0.5322506286306989 1101 30-31 0.5960951392996563 1101 32-33 0.6798633849536415 1101 34-35 0.8204324011574826 1101 36-37 0.7571645014145858 1101 38-39 0.8594675576222315 1101 40-41 0.7217475522220909 1101 42-43 0.7263358323362823 1101 44-45 0.7668828517564066 1101 46-47 0.9598056645358071 1101 48-49 0.9255701481095073 1101 50-51 0.7810244656030036 1101 52-53 1.166311307294606 1101 54-55 1.0120259472019413 1101 56-57 0.8681738991189221 1101 58-59 1.0149706586747271 1101 60-61 1.0336752593128793 1101 62-63 1.0982803453629373 1101 64-65 1.2206678527764154 1101 66-67 1.0515846765314407 1101 68-69 1.2614370935888104 1101 70-71 1.2035454750639119 1101 72-73 1.2272679835998446 1101 74-75 1.2244295034298922 1101 76-77 0.8268855528638532 1101 78-79 0.7335010074746791 1101 80-81 0.8293753401820005 1101 82-83 0.8785423166494049 1101 84-85 1.0273775639185487 1101 86-87 1.0762881424766384 1101 88-89 0.98390287861546 1101 90-91 1.2548975699868912 1101 92-93 0.892488253736694 1101 94-95 0.6175236779948428 1101 96-97 0.5046596019890011 1101 98-99 0.15888715358256178 1101 100-101 0.02440059198702471 1102 1 0.2195045893616019 1102 2 0.33359327345618084 1102 3 0.3961581203392903 1102 4 0.3983360499774662 1102 5 0.38016696669101435 1102 6 0.3788485784104836 1102 7 0.23327662313081987 1102 8 0.24692632712726947 1102 9 0.21221057424979506 1102 10-11 0.13444963295792434 1102 12-13 0.3247788662553006 1102 14-15 0.3751550776338419 1102 16-17 0.4360011059256763 1102 18-19 0.43654471529502104 1102 20-21 0.3092733900147451 1102 22-23 0.38705117984746806 1102 24-25 0.4384232113188844 1102 26-27 0.3423005239009953 1102 28-29 0.619538602711124 1102 30-31 0.6493725381233375 1102 32-33 0.6490927563330828 1102 34-35 0.7277770189013992 1102 36-37 0.786301860082439 1102 38-39 0.876039596572511 1102 40-41 0.7473768154555742 1102 42-43 0.809958367472646 1102 44-45 0.9005472712918277 1102 46-47 1.0535358580904841 1102 48-49 0.9698900474096561 1102 50-51 0.6915713561850012 1102 52-53 0.5007571257246326 1102 54-55 0.569225853239054 1102 56-57 0.7143251882276473 1102 58-59 0.7827028289957596 1102 60-61 0.7879357226562718 1102 62-63 0.7232620624903348 1102 64-65 0.6821076123732652 1102 66-67 0.5763784678895192 1102 68-69 0.4271198092934583 1102 70-71 0.6243573675739285 1102 72-73 0.5113151246017935 1102 74-75 0.39547519509866547 1102 76-77 0.439621069504593 1102 78-79 0.30239087145010757 1102 80-81 0.517225761906932 1102 82-83 0.2706933132321758 1102 84-85 0.47373007543122014 1102 86-87 0.4860926376255854 1102 88-89 0.4270907910881476 1102 90-91 0.4110776241485148 1102 92-93 0.42652320615690087 1102 94-95 0.4919879344248699 1102 96-97 0.48002093803485124 1102 98-99 0.3370930829951604 1102 100-101 0.11031901270140665 1103 1 0.35466232007039267 1103 2 0.39239156986823076 1103 3 0.35757351653728975 1103 4 0.24999871154912512 1103 5 0.3244720717645748 1103 6 0.40351821278689215 1103 7 0.4158826924911807 1103 8 0.3813005474468909 1103 9 0.6384577345717801 1103 10-11 0.6840405880089406 1103 12-13 0.5333655273192122 1103 14-15 0.6792818574936845 1103 16-17 0.6056398594875745 1103 18-19 0.5199557430943216 1103 20-21 0.7185481395321602 1103 22-23 0.5432498850856078 1103 24-25 0.5914134633991281 1103 26-27 0.5512307914763568 1103 28-29 0.7156236770570743 1103 30-31 0.8568605337812087 1103 32-33 0.8021858715091028 1103 34-35 0.369581456459116 1103 36-37 0.6346532054004186 1103 38-39 0.8144202250441381 1103 40-41 0.6980747525033522 1103 42-43 0.8312422739941638 1103 44-45 0.637307477718366 1103 46-47 0.7211895727613893 1103 48-49 0.9023921593859825 1103 50-51 0.8239167660834923 1103 52-53 0.6232692613813597 1103 54-55 0.6138202881267176 1103 56-57 0.35659141542814865 1103 58-59 0.37013427183670444 1103 60-61 0.5706363385217017 1103 62-63 0.5942958147172064 1103 64-65 0.7814514771367413 1103 66-67 0.5576070471969601 1103 68-69 0.3343110339771016 1103 70-71 0.26448535552800223 1103 72-73 0.16302068227297184 1103 74-75 0.5119928197052701 1103 76-77 0.46511360864728246 1103 78-79 0.40249198368468697 1103 80-81 0.5472223914991119 1103 82-83 0.2854136788119952 1103 84-85 0.33496119348741615 1103 86-87 0.17358360974749942 1103 88-89 0.38412684569750866 1103 90-91 0.416264550467897 1103 92-93 0.4843147573761577 1103 94-95 0.71445454769027 1103 96-97 0.5449932087705172 1103 98-99 0.5182306175199578 1103 100-101 0.3868040712895997 1104 1 -0.024094189120901888 1104 2 -0.16887325226615957 1104 3 -0.06444794026677414 1104 4 -0.12292305267698111 1104 5 -0.09373484209451277 1104 6 -0.2316282832291634 1104 7 -0.04381992373317445 1104 8 0.021039552403287587 1104 9 0.03631205416522931 1104 10-11 -0.005155722926495798 1104 12-13 -0.03836419223659249 1104 14-15 0.11043814082388081 1104 16-17 0.1714720948850328 1104 18-19 0.06630745901126289 1104 20-21 0.07168823615051423 1104 22-23 0.11124662264756324 1104 24-25 0.11270087164306375 1104 26-27 -0.05626654210793447 1104 28-29 -0.14510786193533676 1104 30-31 -0.007240099884647577 1104 32-33 0.10359122943620491 1104 34-35 0.1855172115253083 1104 36-37 0.15207587934482802 1104 38-39 0.5063896083178676 1104 40-41 0.23325889203531602 1104 42-43 0.3738296383206645 1104 44-45 0.3098026154308897 1104 46-47 0.16030118578603947 1104 48-49 -0.18672259059832896 1104 50-51 -0.36614534571775437 1104 52-53 -0.07685621700499468 1104 54-55 -0.0202972851212877 1104 56-57 0.08578396201200178 1104 58-59 -0.028196865624863676 1104 60-61 0.16914314572415634 1104 62-63 0.09974785060402525 1104 64-65 -0.07311907308597654 1104 66-67 0.10254713126747816 1104 68-69 0.10405192110929562 1104 70-71 -0.23422849101193322 1104 72-73 -0.18605391462771337 1104 74-75 -0.030763940442795956 1104 76-77 -0.13919030000445431 1104 78-79 -0.33040217529642746 1104 80-81 -0.13393702879074354 1104 82-83 0.2806768697273547 1104 84-85 0.12977893613124536 1104 86-87 -0.1974873482799815 1104 88-89 -0.057663730875678 1104 90-91 -0.12223221846365462 1104 92-93 0.39361270580453933 1104 94-95 0.04773141574974815 1104 96-97 -0.0742426278566235 1104 98-99 -0.12558016107343306 1104 100-101 -0.2874728595090694 1105 1 0.15929613434724743 1105 2 0.2603214181736462 1105 3 0.33991475281187533 1105 4 0.42274766225443017 1105 5 0.3422436195648828 1105 6 0.37039191879103583 1105 7 0.441476621485549 1105 8 0.4021817133119825 1105 9 0.445862814688752 1105 10-11 0.38650210044338706 1105 12-13 0.3833667357200099 1105 14-15 0.4849065604284917 1105 16-17 0.51593422592304 1105 18-19 0.5279432723980406 1105 20-21 0.630745737231365 1105 22-23 0.4353396934332423 1105 24-25 0.505673398467728 1105 26-27 0.4091188291140142 1105 28-29 0.5207589776669082 1105 30-31 0.7109259968806683 1105 32-33 0.552439635677608 1105 34-35 0.6212893295931465 1105 36-37 0.6968858467228856 1105 38-39 0.7451546910518232 1105 40-41 0.8258148013227427 1105 42-43 0.49847688903795273 1105 44-45 0.42750493040946935 1105 46-47 0.23759159965233323 1105 48-49 0.12102610255892188 1105 50-51 0.23774570486590818 1105 52-53 0.07821000946201195 1105 54-55 0.0492070972235652 1105 56-57 0.0787800709614217 1105 58-59 -0.03151801486040995 1105 60-61 -0.03700196295013569 1105 62-63 0.10567089427026843 1105 64-65 0.1189376812275853 1105 66-67 0.23450484065710242 1105 68-69 0.054183587972474356 1105 70-71 0.1497376293355117 1105 72-73 0.44608655622871396 1105 74-75 0.6203581756598666 1105 76-77 0.4963970914195137 1105 78-79 0.6154847091118576 1105 80-81 0.35244429416645673 1105 82-83 0.3875235466129432 1105 84-85 0.2495977468060886 1105 86-87 0.33042799189216865 1105 88-89 0.36535635323389926 1105 90-91 0.3094660875537194 1105 92-93 0.175248126155914 1105 94-95 0.002495004949135904 1105 96-97 -0.28220749049074456 1105 98-99 -0.06668773486583035 1105 100-101 -0.34362386371454434 1106 1 0.43769931847413446 1106 2 0.47622274588327684 1106 3 0.5573309170082688 1106 4 0.6214288634306584 1106 5 0.5435386562476907 1106 6 0.47344535390553943 1106 7 0.5084932846869066 1106 8 0.6242240693282639 1106 9 0.5251392574962495 1106 10-11 0.6087003955097714 1106 12-13 0.6241329162528828 1106 14-15 0.8070357967726878 1106 16-17 0.8530261854085879 1106 18-19 0.7853355259400914 1106 20-21 0.7823964634618008 1106 22-23 0.8074028413228618 1106 24-25 0.8906386463365621 1106 26-27 0.7712278676383804 1106 28-29 0.6217072356049513 1106 30-31 0.6271495986271916 1106 32-33 0.7679177002512247 1106 34-35 0.9324657040844855 1106 36-37 0.7553741290171203 1106 38-39 0.8139460891567865 1106 40-41 0.6491024420391724 1106 42-43 0.3923603654391741 1106 44-45 0.5545766920752015 1106 46-47 0.42201180341268696 1106 48-49 0.3784443459426541 1106 50-51 0.22335847107613915 1106 52-53 0.10568869779182677 1106 54-55 0.23477827968709875 1106 56-57 0.3632648780425356 1106 58-59 0.5169010601982507 1106 60-61 0.4492456979641162 1106 62-63 0.7953373332164162 1106 64-65 0.9292712422814304 1106 66-67 0.6340318255384361 1106 68-69 0.47098267907058755 1106 70-71 0.6505544529313099 1106 72-73 0.7007164608831218 1106 74-75 0.7559100513838679 1106 76-77 0.889040481088923 1106 78-79 1.029068485510109 1106 80-81 1.1570935055604004 1106 82-83 0.9580228486000557 1106 84-85 0.741422085178975 1106 86-87 1.0577462857704454 1106 88-89 0.9948711600179934 1106 90-91 1.0461481501075554 1106 92-93 1.359267343787426 1106 94-95 1.1205223017085721 1106 96-97 1.231158713804259 1106 98-99 0.988707756267921 1106 100-101 1.1842038310607528 1107 1 0.32120788825664803 1107 2 0.3452491033656315 1107 3 0.3419557189670748 1107 4 0.14307656615282127 1107 5 0.3127450511778349 1107 6 0.330769277281604 1107 7 0.3305082638025638 1107 8 0.33342758365924396 1107 9 0.40938238006574323 1107 10-11 0.38535313928047543 1107 12-13 0.20706176545350985 1107 14-15 0.3097615637322093 1107 16-17 0.5361566765507391 1107 18-19 0.5002030183785138 1107 20-21 0.4979985086872105 1107 22-23 0.5955995862458039 1107 24-25 0.41181465094552294 1107 26-27 0.5345758440174748 1107 28-29 0.4840325992805887 1107 30-31 0.5427408416057276 1107 32-33 0.5293392759389732 1107 34-35 0.4750445604866158 1107 36-37 0.4012944804502254 1107 38-39 0.7630314989220182 1107 40-41 0.6132569861843606 1107 42-43 0.6596691656696194 1107 44-45 0.411536939806723 1107 46-47 0.20592725782721288 1107 48-49 0.2238929992625387 1107 50-51 0.23574144673507647 1107 52-53 0.11620648549167356 1107 54-55 0.2655898885017365 1107 56-57 0.01785943371011456 1107 58-59 0.3499811408550286 1107 60-61 0.34897924254138957 1107 62-63 0.43664512523715615 1107 64-65 0.5530577898833329 1107 66-67 0.35085092391089745 1107 68-69 0.7397389803812615 1107 70-71 0.9161240914161155 1107 72-73 0.7217124280442917 1107 74-75 0.8091255202013805 1107 76-77 0.569023917643726 1107 78-79 0.684444403701093 1107 80-81 0.7000252353601972 1107 82-83 0.610932253756328 1107 84-85 0.5994949643378362 1107 86-87 0.5862462137554658 1107 88-89 0.6132529834372633 1107 90-91 0.5512288068841649 1107 92-93 0.6782324885375317 1107 94-95 0.5456159211814224 1107 96-97 0.6002570862657315 1107 98-99 0.5832058118634826 1107 100-101 0.6532265877941548 1108 1 0.3501546281658925 1108 2 0.4677643717132369 1108 3 0.41769354693800764 1108 4 0.46673482812013845 1108 5 0.37979612763898984 1108 6 0.3541170544149068 1108 7 0.5851570065095828 1108 8 0.5438919461417555 1108 9 0.6878326854326957 1108 10-11 0.6708320024283125 1108 12-13 0.7379413587525008 1108 14-15 0.6629414743398669 1108 16-17 0.7099326498012815 1108 18-19 0.6387150795201464 1108 20-21 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-2.3890842176580165 2316 74-75 -1.9023645620434166 2316 76-77 -1.5279496887638437 2316 78-79 -1.772412017306273 2316 80-81 -1.7051621000158157 2316 82-83 -1.5827004936500053 2316 84-85 -1.6366416802893724 2316 86-87 -1.5707714715641075 2316 88-89 -1.9668711900831397 2316 90-91 -1.7507869970184302 2316 92-93 -1.5829995708077327 2316 94-95 -1.7590284910101595 2316 96-97 -2.095351148965147 2316 98-99 -2.089177674670946 2316 100-101 -1.5806093526455633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3153.0 3 523.0 4 555.0 5 505.0 6 546.0 7 615.0 8 878.0 9 994.0 10 1269.0 11 1838.0 12 2726.0 13 4095.0 14 4127.0 15 5007.0 16 6808.0 17 9301.0 18 7129.0 19 4270.0 20 3997.0 21 3848.0 22 4130.0 23 4529.0 24 4769.0 25 5226.0 26 6061.0 27 6954.0 28 8161.0 29 9569.0 30 11360.0 31 13730.0 32 16876.0 33 21202.0 34 28278.0 35 40613.0 36 57714.0 37 97858.0 38 129808.0 39 28271.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.45632289190449 23.02506914664848 15.777672605690448 20.74093535575658 2 14.665893050568265 23.330453230056783 40.96117608807727 21.042477631297682 3 17.284658156894803 28.766562165949534 32.567034664894045 21.381745012261614 4 11.52923675601093 19.661666765478945 41.104110411041106 27.704986067469022 5 10.925012936238716 39.62262116943598 36.47444374173518 12.977922152590121 6 25.909488338189803 40.9135548457869 19.23646607024555 13.940490745777744 7 23.53951951736273 36.045534527956335 24.434409451646495 15.980536503034436 8 22.93105027176597 35.753340288339466 22.769983821530474 18.545625618364088 9 25.030569316486247 18.08823714248264 24.455094078081487 32.42609946294963 10-11 21.719470012469554 28.896960534619847 30.176952778179267 19.20661667473133 12-13 23.262373908765426 28.048992886102226 29.75717225256671 18.931460952565633 14-15 20.964791008330938 29.477879919563343 28.94929617925883 20.608032892846882 16-17 17.986754681511766 32.182245671301715 30.33361073630289 19.497388910883622 18-19 17.638102078376804 30.652870673789113 33.888045966692104 17.820981281141986 20-21 18.792029463046394 29.689208674432262 33.691805186200064 17.826956676321284 22-23 18.85871497685177 29.52028029293557 33.17299576543605 18.448008964776616 24-25 18.46379448294317 29.88727560409877 33.650562454595494 17.998367458362555 26-27 18.176462876057162 29.956698760948786 34.03439673705139 17.832441625942668 28-29 17.92333387209741 29.73869942352244 34.199576172260834 18.13839053211932 30-31 18.476812510325903 29.773710070180226 33.691806799652326 18.057670619841538 32-33 17.98472983625515 30.401883272311338 33.49550999007008 18.117876901363434 34-35 18.406670737552847 29.954018171500618 33.535168949270705 18.104142141675833 36-37 18.748137296655823 29.558074475719355 33.03154912989806 18.66223909772676 38-39 17.978012560907437 30.37067909374952 33.244727056360254 18.40658128898279 40-41 18.1014053830265 30.076096788274526 33.690221720210424 18.132276108488547 42-43 18.093924641355073 29.956663737973148 32.99011205853546 18.959299562136316 44-45 18.22676907591291 30.13945171859005 32.614271810657534 19.0195073948395 46-47 18.671588709314367 30.208616575878988 32.08103362895602 19.038761085850627 48-49 18.59638159316515 30.15152934057543 31.978381766053392 19.273707300206024 50-51 18.290022339054808 30.428205735401505 31.67220565976746 19.609566265776227 52-53 18.28254124324918 30.79588981150831 31.782108146363335 19.13946079887917 54-55 18.6783259719939 30.120602327419654 31.565201421011597 19.63587027957485 56-57 18.552072447683624 30.627616493891434 31.31529702128119 19.50501403714375 58-59 18.47485953577282 29.834934846018086 31.841618090298496 19.8485875279106 60-61 18.63460036776745 30.22017467154624 31.475693882177673 19.669531078508633 62-63 18.352161071457186 29.93002724913378 32.01957760691932 19.69823407248972 64-65 18.4396019990095 29.846742605015532 31.73940840124263 19.97424699473234 66-67 18.303016659162537 29.90751913552454 31.222962629446194 20.566501575866727 68-69 18.143559095510334 29.63817531421902 31.684876780034077 20.53338881023657 70-71 18.53189427534953 29.531519620734255 31.657504440017437 20.279081663898786 72-73 18.519255566681313 28.91126447048993 31.743770575649954 20.825709387178804 74-75 18.243289485058927 29.746977926980378 31.109162032100972 20.900570555859723 76-77 18.37014360959798 29.357853509206322 31.51154729212623 20.760455589069462 78-79 18.27915078247015 29.134354422745417 31.52789842515787 21.058596369626567 80-81 18.495724187595645 29.15852011882258 31.187145557655953 21.158610135925827 82-83 18.657727546492396 28.92922316260838 31.229063003872078 21.183986287027142 84-85 18.438628240103 28.564495943962765 31.244361613051346 21.752514202882892 86-87 18.55434637780843 28.51217720415858 31.394697703562592 21.5387787144704 88-89 19.087790383576444 28.072573383756527 31.296686475778856 21.54294975688817 90-91 18.988229848039044 27.7357340156401 31.483828086794176 21.792208049526682 92-93 19.343540955969495 27.886226013407633 31.26551226031419 21.504720770308676 94-95 19.267003541963668 27.244301833301666 31.477727415591307 22.010967209143363 96-97 19.600954294550633 26.950715495758544 31.007367953679427 22.4409622560114 98-99 19.584124285441558 27.109005507548112 30.903414391666278 22.40345581534405 100-101 19.977077683783676 26.381821324776627 31.492885618902537 22.148215372537155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1752.0 1 1051.5 2 391.5 3 408.5 4 322.5 5 359.5 6 620.0 7 1032.5 8 1423.0 9 1491.5 10 1291.0 11 1160.5 12 1223.5 13 1269.5 14 1283.5 15 1345.0 16 1445.0 17 1546.5 18 1680.0 19 1922.5 20 2203.5 21 2464.0 22 2753.0 23 3140.5 24 3609.0 25 4339.0 26 5730.0 27 7653.5 28 8682.0 29 9779.0 30 11913.0 31 13797.5 32 16507.0 33 19825.5 34 22826.5 35 25282.0 36 27806.0 37 29742.5 38 28645.5 39 26170.0 40 24135.5 41 22537.0 42 20706.0 43 19579.0 44 18987.0 45 17877.0 46 16463.0 47 15463.5 48 16553.5 49 15213.0 50 11041.5 51 8327.0 52 6792.0 53 5885.0 54 5097.0 55 4351.5 56 3830.5 57 3380.0 58 2969.0 59 2751.5 60 2477.0 61 2141.0 62 1995.5 63 1889.5 64 1717.0 65 1606.0 66 1430.5 67 1224.0 68 1115.5 69 965.0 70 813.0 71 689.5 72 564.0 73 457.0 74 350.5 75 265.0 76 192.0 77 140.5 78 95.0 79 65.0 80 50.5 81 34.0 82 22.0 83 12.0 84 9.5 85 7.5 86 4.0 87 3.5 88 2.5 89 0.5 90 1.0 91 1.0 92 1.5 93 1.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28620235493398505 2 0.1085595139404771 3 0.12255561656420802 4 0.12327336541670703 5 0.12955366787607348 6 0.05544609885554945 7 0.06423852229866248 8 0.06908332705303087 9 0.06585345721678527 10-11 0.06127780828210401 12-13 0.06459739672491199 14-15 0.06064977803616736 16-17 0.06217499434772779 18-19 0.06693008049553381 20-21 0.07123657361052795 22-23 0.0811056203323895 24-25 0.08927001352956586 26-27 0.0898980437755025 28-29 0.08397661574238557 30-31 0.08002899705364096 32-33 0.07087769918427843 34-35 0.2364085282918654 36-37 0.3569903355117011 38-39 0.33097193960861154 40-41 0.31500202764050833 42-43 0.33626533739579184 44-45 0.3489156609210871 46-47 0.35402962149514267 48-49 0.3635397937907547 50-51 0.35752864715107535 52-53 0.35797724018388727 54-55 0.34434001198640585 56-57 0.32433276272299555 58-59 0.31033666009926464 60-61 0.27130906624463036 62-63 0.33509899551048095 64-65 0.3630014821513804 66-67 0.36748741247949934 68-69 0.37897139411948366 70-71 0.3804068918244817 72-73 0.3642575426432537 74-75 0.36784628690574883 76-77 0.35402962149514267 78-79 0.3311513768217363 80-81 0.3315999698545482 82-83 0.34299423288797015 84-85 0.35044087723264755 86-87 0.3438017003470316 88-89 0.3585155518232615 90-91 0.3808554848572936 92-93 0.37538264985698855 94-95 0.3763695545291747 96-97 0.38103492207041834 98-99 0.36964065903699633 100-101 0.34882594231452474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.99912998579191 #Duplication Level Percentage of deduplicated Percentage of total 1 78.14030212772155 35.162456125743944 2 11.207842350910774 10.08686309617795 3 3.874417764530099 5.230362858160538 4 1.8404949898297818 3.3128269314219647 5 1.0557148076489549 2.375312392866032 6 0.6567560792244918 1.7732071306789172 7 0.44120871093805186 1.389780569405556 8 0.33909715409433966 1.2207261531922642 9 0.248948248337299 1.0082209127998791 >10 1.8098924052491794 16.611526366265192 >50 0.23525546351732626 7.384430693256125 >100 0.144895073929238 11.141220622069351 >500 0.002786443729408423 0.7926284004735986 >1k 0.002388380339492934 2.510437747488697 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4615 0.8281027385707468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3322 0.5960904220004378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2322 0.4166532088756823 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1643 0.2948153411639733 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1060 0.19020344591224084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1053 0.18894738542036757 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 0.146061891483551 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 796 0.1428320216473054 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 695 0.12470886312170508 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.971860656237776E-5 0.0 0.0 0.0 0.0 12-13 1.794372131247555E-4 0.0 0.0 1.794372131247555E-4 0.0 14-15 1.794372131247555E-4 0.0 0.0 1.794372131247555E-4 0.0 16-17 1.794372131247555E-4 0.0 0.0 1.794372131247555E-4 0.0 18-19 1.794372131247555E-4 0.0 0.0 2.6915581968713325E-4 0.0 20-21 1.794372131247555E-4 0.0 0.0 6.280302459366443E-4 0.0 22-23 1.794372131247555E-4 0.0 0.0 0.0017943721312475552 1.794372131247555E-4 24-25 1.794372131247555E-4 0.0 0.0 0.006190583852804066 1.794372131247555E-4 26-27 1.794372131247555E-4 0.0 0.0 0.013726946804043797 1.794372131247555E-4 28-29 1.794372131247555E-4 0.0 0.0 0.03086320065745795 1.794372131247555E-4 30-31 1.794372131247555E-4 0.0 0.0 0.07652997139770823 1.794372131247555E-4 32-33 1.794372131247555E-4 0.0 0.0 0.16597942214039885 1.794372131247555E-4 34-35 1.794372131247555E-4 0.0 0.0 0.31643752534550634 1.794372131247555E-4 36-37 1.794372131247555E-4 0.0 0.0 0.5354406439642705 1.794372131247555E-4 38-39 1.794372131247555E-4 0.0 0.0 0.8049553380776533 1.794372131247555E-4 40-41 1.794372131247555E-4 0.0 0.0 1.0896324767000778 1.794372131247555E-4 42-43 1.794372131247555E-4 0.0 0.0 1.374668489748752 1.794372131247555E-4 44-45 1.794372131247555E-4 0.0 0.0 1.7284289554242076 1.794372131247555E-4 46-47 1.794372131247555E-4 0.0 0.0 2.150644717906757 1.794372131247555E-4 48-49 1.794372131247555E-4 0.0 0.0 2.569810047766186 1.794372131247555E-4 50-51 1.794372131247555E-4 0.0 0.0 2.9909491869699876 1.794372131247555E-4 52-53 1.794372131247555E-4 0.0 0.0 3.3890306442872573 1.794372131247555E-4 54-55 1.794372131247555E-4 0.0 0.0 3.8383414259516453 1.794372131247555E-4 56-57 1.794372131247555E-4 0.0 0.0 4.38185674450653 1.794372131247555E-4 58-59 1.794372131247555E-4 0.0 0.0 4.953184831095751 1.794372131247555E-4 60-61 1.794372131247555E-4 0.0 0.0 5.547750036784629 1.794372131247555E-4 62-63 1.794372131247555E-4 0.0 0.0 6.132356477145082 1.794372131247555E-4 64-65 1.794372131247555E-4 0.0 0.0 6.756797978819231 1.794372131247555E-4 66-67 1.794372131247555E-4 0.0 0.0 7.4880046223026095 1.794372131247555E-4 68-69 1.794372131247555E-4 0.0 0.0 8.292152492921202 1.794372131247555E-4 70-71 1.794372131247555E-4 0.0 0.0 9.165383690592824 1.794372131247555E-4 72-73 1.794372131247555E-4 0.0 0.0 9.993217273343884 1.794372131247555E-4 74-75 1.794372131247555E-4 0.0 0.0 10.874702582819246 1.794372131247555E-4 76-77 1.794372131247555E-4 0.0 0.0 11.828052496151072 1.794372131247555E-4 78-79 1.794372131247555E-4 0.0 0.0 12.888795581538066 2.6915581968713325E-4 80-81 1.794372131247555E-4 0.0 0.0 14.013956626436844 3.58874426249511E-4 82-83 1.794372131247555E-4 0.0 0.0 15.094617242480684 3.58874426249511E-4 84-85 1.794372131247555E-4 0.0 0.0 16.201924284673552 3.58874426249511E-4 86-87 1.794372131247555E-4 0.0 0.0 17.39724527990411 3.58874426249511E-4 88-89 1.794372131247555E-4 0.0 0.0 18.64092460407179 3.58874426249511E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 25 0.0050095306 57.029823 1 CCCTATA 170 3.6925485E-9 27.898014 2 CGGAAAC 35 0.008340694 27.176636 34-35 CGGGGAT 70 0.008451401 27.10093 4 GTACAGG 125 7.351973E-6 26.613918 1 ACTCCCG 95 0.0012459544 24.93002 6 GGTATCA 3840 0.0 24.752527 1 GTATCAA 5160 0.0 24.130836 1 CCTATAC 295 0.0 24.115234 3 GTACATG 2540 0.0 22.639791 1 GAGTACT 3410 0.0 22.57632 12-13 ACACAGT 215 4.5440174E-8 22.031178 6 CATTCCG 155 3.9870283E-5 21.391565 9 GTACTTT 3615 0.0 21.231483 14-15 TACATGG 2685 0.0 21.196257 2 AGTACTT 3525 0.0 20.898996 12-13 GTATAGG 320 1.8189894E-11 20.792126 1 GTGGTAC 115 0.0037096306 20.66298 1 CATGGGG 920 0.0 20.620272 4 CAACGCA 6045 0.0 20.55546 5 >>END_MODULE