FastQCFastQC Report
Fri 27 May 2016
SRR939318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557298
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.8272055525051228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.6176228875754084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.43513524182753216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16910.3034283273939616No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT12410.2226815814878216No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11060.19845755771597962No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.1964837483716073No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.16562054771414933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.14031990066355882No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT6980.12524717476107936RNA PCR Primer, Index 17 (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6920.12417055148233082No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5650.10138202541548687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT459.659665E-442.1876454
ACGCCGA702.0618773E-433.900796
AGGTAAC600.003904495631.7404121
ACACGGA600.003965089631.6407386
TACGCCG753.0912456E-431.6407345
CATGGGG7950.029.2527564
CGGAGGT650.005862381329.2094549
TACACGG650.005864952729.2068355
CGCCGAA1056.93814E-527.1206327
GTACATG24600.025.7407021
GTGCTAG1151.2605233E-424.8403221
ACAGTCC1151.2877377E-424.7623148
GTATCAA59000.024.6122681
GGTACTC1551.4185407E-624.4960548
ACATGGG23300.024.4434873
ATGGGGA5250.024.4085675
GGTATCA43700.024.1866321
TACATGG25350.023.9645822
GTGTAGC1000.001630349423.8053111
TGTTCGC1000.001660478523.7305538
Produced by FastQC (version 0.11.5)