##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557298 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18336868246432 33.0 31.0 34.0 30.0 34.0 2 32.38539883509361 34.0 31.0 34.0 30.0 34.0 3 32.46299466353728 34.0 31.0 34.0 30.0 34.0 4 36.03550165261673 37.0 35.0 37.0 35.0 37.0 5 35.97294625137718 37.0 35.0 37.0 35.0 37.0 6 36.01656026039928 37.0 35.0 37.0 35.0 37.0 7 35.965045630883296 37.0 35.0 37.0 35.0 37.0 8 35.954035363485964 37.0 35.0 37.0 35.0 37.0 9 37.628527287017 39.0 37.0 39.0 35.0 39.0 10-11 37.56678653072504 39.0 37.0 39.0 35.0 39.0 12-13 37.51503683845985 39.0 37.0 39.0 35.0 39.0 14-15 38.758435343388996 40.0 38.0 41.0 34.0 41.0 16-17 38.72380665281412 40.0 38.0 41.0 34.0 41.0 18-19 38.86066431245043 40.0 38.0 41.0 35.0 41.0 20-21 38.877156386708734 40.0 38.0 41.0 35.0 41.0 22-23 38.805988896425255 40.0 38.0 41.0 34.0 41.0 24-25 38.73616269930989 40.0 38.0 41.0 34.0 41.0 26-27 38.53820397704639 40.0 38.0 41.0 34.0 41.0 28-29 38.369296139587796 40.0 38.0 41.0 34.0 41.0 30-31 38.11124479183489 40.0 37.5 41.0 33.5 41.0 32-33 37.81483694540444 40.0 36.5 41.0 33.0 41.0 34-35 37.65446493617418 40.0 36.0 41.0 33.0 41.0 36-37 37.503384185839536 40.0 36.0 41.0 33.0 41.0 38-39 37.327600314373996 40.0 36.0 41.0 33.0 41.0 40-41 37.09719575523329 40.0 36.0 41.0 32.0 41.0 42-43 36.81791788235378 40.0 36.0 41.0 31.0 41.0 44-45 36.44304752574027 40.0 35.0 41.0 30.5 41.0 46-47 35.93936457694089 40.0 35.0 41.0 29.0 41.0 48-49 35.483460374880224 39.5 35.0 41.0 26.5 41.0 50-51 35.18223822802163 39.0 35.0 41.0 24.5 41.0 52-53 34.95948846039282 39.0 35.0 41.0 24.0 41.0 54-55 34.704424742238444 39.0 35.0 40.5 23.0 41.0 56-57 34.46375009420454 39.0 35.0 40.0 22.5 41.0 58-59 34.179879525855114 38.0 34.0 40.0 20.0 41.0 60-61 33.85299606314754 38.0 34.0 40.0 17.0 41.0 62-63 33.48982949876009 38.0 34.0 40.0 12.5 41.0 64-65 33.08624290774415 37.5 33.0 40.0 7.5 41.0 66-67 32.712094785913465 37.0 33.0 39.5 4.0 41.0 68-69 32.31168064482557 36.5 32.0 39.0 2.0 40.5 70-71 31.966360008469437 36.0 32.0 39.0 2.0 40.0 72-73 31.484070461404848 35.5 31.5 38.5 2.0 40.0 74-75 30.98895205078791 35.0 31.0 37.5 2.0 40.0 76-77 28.973080829287024 33.5 28.5 35.5 2.0 38.5 78-79 29.894143707675248 34.5 30.0 36.0 2.0 39.0 80-81 29.869271915564024 35.0 30.0 36.0 2.0 38.5 82-83 29.527558864377767 34.0 30.0 36.0 2.0 37.0 84-85 29.132273038840978 34.0 29.5 35.0 2.0 37.0 86-87 28.612078277689854 34.0 29.0 35.0 2.0 36.5 88-89 28.08523895653672 34.0 29.0 35.0 2.0 36.0 90-91 27.66998715229554 34.0 27.5 35.0 2.0 36.0 92-93 27.34036816927389 34.0 27.0 35.0 2.0 35.0 94-95 26.986884036906645 33.5 26.0 35.0 2.0 35.0 96-97 26.496452526296522 33.0 25.5 35.0 2.0 35.0 98-99 25.83772954505489 33.0 24.0 35.0 2.0 35.0 100-101 22.874323073113487 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.336031125155408 1101 2 0.32141625607450663 1101 3 0.22202965917750817 1101 4 0.3042815744851808 1101 5 0.2830126632943788 1101 6 0.2973536299937436 1101 7 0.2548115476227437 1101 8 0.17831176096554913 1101 9 0.27489996939561934 1101 10-11 0.2838400745005316 1101 12-13 0.28484634554354216 1101 14-15 0.23426598046060576 1101 16-17 0.4085861751301678 1101 18-19 0.3340079398915563 1101 20-21 0.32479649300071145 1101 22-23 0.3888678299372117 1101 24-25 0.4309561357475573 1101 26-27 0.3984784541147519 1101 28-29 0.3202600228444439 1101 30-31 0.35382254562853177 1101 32-33 0.3737974425154462 1101 34-35 0.6068199850732228 1101 36-37 0.7479550307278942 1101 38-39 0.7657594004329482 1101 40-41 0.6825431239535291 1101 42-43 0.5989188122217897 1101 44-45 0.5057299429063207 1101 46-47 0.6441123820224632 1101 48-49 0.3741987034252716 1101 50-51 0.2872659590366382 1101 52-53 0.26501529815081426 1101 54-55 0.13364395921770011 1101 56-57 0.32103799285700774 1101 58-59 0.08144319017550572 1101 60-61 0.6554556697364333 1101 62-63 0.5633202672516475 1101 64-65 0.42626717629070754 1101 66-67 0.5069181490831198 1101 68-69 0.286575833049973 1101 70-71 0.24839658148195554 1101 72-73 0.4575473325325419 1101 74-75 0.5879246939603497 1101 76-77 0.4922524964607433 1101 78-79 0.42820266997109613 1101 80-81 0.5233894187748618 1101 82-83 0.40292296854624965 1101 84-85 0.44808021903557815 1101 86-87 0.7121005951683053 1101 88-89 0.6572713534607146 1101 90-91 0.8647580621225934 1101 92-93 0.7375882055950846 1101 94-95 1.2061661766161471 1101 96-97 1.0573759714746132 1101 98-99 1.44150552392205 1101 100-101 1.2736511783824263 1102 1 0.06363183608213774 1102 2 0.1457854415669928 1102 3 0.09276864180766609 1102 4 0.19318376000869364 1102 5 0.1563888117911958 1102 6 0.1226535799097519 1102 7 0.13415788506473802 1102 8 0.22180062060184014 1102 9 0.3185705497050719 1102 10-11 0.17916453454635928 1102 12-13 0.1110172250272754 1102 14-15 0.3444596237828108 1102 16-17 0.38878305396679025 1102 18-19 0.39191256756361526 1102 20-21 0.23907973791141757 1102 22-23 0.3082769274946173 1102 24-25 0.36074990494457637 1102 26-27 0.3251851879737444 1102 28-29 0.30713624447275123 1102 30-31 0.4203300959008871 1102 32-33 0.45073841875436216 1102 34-35 0.6514434908639046 1102 36-37 0.6697796835749301 1102 38-39 0.6830831424605037 1102 40-41 0.8932526766990634 1102 42-43 0.9939485302001643 1102 44-45 0.9326582873423419 1102 46-47 0.9601246326551589 1102 48-49 1.0623704604868962 1102 50-51 1.2511390027692713 1102 52-53 1.3355339998674083 1102 54-55 1.4642204569727753 1102 56-57 1.5392712246926905 1102 58-59 1.4104573333625297 1102 60-61 1.5995987237961202 1102 62-63 1.563790081674945 1102 64-65 1.1951472894007509 1102 66-67 1.0492413370842328 1102 68-69 0.8299583196092968 1102 70-71 0.9936618715037113 1102 72-73 1.2555293466181148 1102 74-75 1.4865489354981456 1102 76-77 1.486113883477021 1102 78-79 1.4280807841537282 1102 80-81 1.5216586401682584 1102 82-83 1.4649273918933687 1102 84-85 1.3327585069609071 1102 86-87 1.1687624307243922 1102 88-89 1.4152304973347363 1102 90-91 1.0237725155644277 1102 92-93 1.1221778492895993 1102 94-95 1.0851402082887311 1102 96-97 0.9824206260058865 1102 98-99 0.7691877875411244 1102 100-101 0.8486043077453829 1103 1 0.12436921127856237 1103 2 0.14048470926928758 1103 3 0.16760044970317978 1103 4 0.2190308485453656 1103 5 0.16570339340102436 1103 6 0.0860693350343027 1103 7 0.27883592357493114 1103 8 0.1434145644604783 1103 9 0.056506021715371446 1103 10-11 0.18812018960919374 1103 12-13 0.24671334555656443 1103 14-15 0.30635212965914604 1103 16-17 0.1353984204001648 1103 18-19 0.09625776735877167 1103 20-21 0.24625826885132795 1103 22-23 0.11698220940515824 1103 24-25 0.11172491577803356 1103 26-27 0.2965148357442473 1103 28-29 0.2443000244070106 1103 30-31 0.17382853544584265 1103 32-33 0.3130528821810614 1103 34-35 0.20690201952768916 1103 36-37 0.6837012187300289 1103 38-39 0.6786140692351168 1103 40-41 0.5961074558935806 1103 42-43 0.487586338297028 1103 44-45 0.7336685342575393 1103 46-47 0.3859121919108688 1103 48-49 0.5227331513854807 1103 50-51 0.7492371297854916 1103 52-53 0.7302678470785082 1103 54-55 0.635623203198584 1103 56-57 0.8089541875000279 1103 58-59 0.7083882531336911 1103 60-61 0.8022061938454499 1103 62-63 0.644410674429011 1103 64-65 0.7559350018568125 1103 66-67 0.6062384350317842 1103 68-69 0.4164311659405868 1103 70-71 0.298053469120795 1103 72-73 0.4473646431045708 1103 74-75 0.5168607201072071 1103 76-77 0.5758039372722301 1103 78-79 0.5821084214978463 1103 80-81 0.4405281090882873 1103 82-83 0.6312001147889461 1103 84-85 0.4932577650334693 1103 86-87 0.27472673941872827 1103 88-89 0.5806212238983619 1103 90-91 0.5245494602240797 1103 92-93 0.5023576627353386 1103 94-95 0.5123109478962746 1103 96-97 0.37400292304824134 1103 98-99 0.4653345797445425 1103 100-101 0.8447086415645764 1104 1 0.04775210857449963 1104 2 -0.02034093851288077 1104 3 0.012195468211238847 1104 4 -0.14123014649823773 1104 5 -0.0038179065550565383 1104 6 -0.12921393242287849 1104 7 -0.11324639024651617 1104 8 0.029693502913325176 1104 9 -0.08763100068252072 1104 10-11 -0.13272177036319732 1104 12-13 -0.04698136552945442 1104 14-15 -0.1183545646127726 1104 16-17 -0.13252303012998823 1104 18-19 -0.0730627671791595 1104 20-21 -0.005106308600211662 1104 22-23 -0.0837832087515622 1104 24-25 -0.12170252634506795 1104 26-27 -0.012118077236493718 1104 28-29 0.0696215627720207 1104 30-31 -0.08721042559312053 1104 32-33 -0.31916996743309056 1104 34-35 -0.6103136150792565 1104 36-37 -0.19366875429211206 1104 38-39 -0.15802561957848837 1104 40-41 -0.13310391244822029 1104 42-43 -0.034052409428674935 1104 44-45 -0.17349628160292951 1104 46-47 0.12667384571600593 1104 48-49 -0.1445358115356541 1104 50-51 -0.10486287648841852 1104 52-53 -0.4932656242810509 1104 54-55 -0.3464208397150301 1104 56-57 -0.5557487424202989 1104 58-59 -0.6459438881549744 1104 60-61 -0.7523392607000048 1104 62-63 -0.7892049852736065 1104 64-65 -0.5915334716986109 1104 66-67 -0.6134553977055219 1104 68-69 -0.8888577480439821 1104 70-71 -1.0799815393489993 1104 72-73 -1.3126642169242935 1104 74-75 -1.378684797177442 1104 76-77 -1.2783211646789567 1104 78-79 -0.905111604852614 1104 80-81 -0.9292692433177621 1104 82-83 -1.1056152437655484 1104 84-85 -1.1469455099524168 1104 86-87 -0.9207372169147909 1104 88-89 -0.6721549853995867 1104 90-91 -0.7311252997985065 1104 92-93 -0.5368543329983986 1104 94-95 -0.8037251898789926 1104 96-97 -1.045063517757331 1104 98-99 -1.0796494217612 1104 100-101 -0.9769301061611237 1105 1 0.0598929724663364 1105 2 0.054740616335209324 1105 3 0.026600216079025074 1105 4 0.02720124917050981 1105 5 -0.028736351706967866 1105 6 0.10154894931276459 1105 7 0.020161619815191045 1105 8 0.08244671597782371 1105 9 0.15462816686846992 1105 10-11 0.08487442505401077 1105 12-13 0.18303867927658501 1105 14-15 0.19414003115031164 1105 16-17 -0.024986576095471946 1105 18-19 0.27131879159929184 1105 20-21 0.32899344378748197 1105 22-23 0.17263309435735152 1105 24-25 0.20268728617704568 1105 26-27 0.32659394571676614 1105 28-29 0.24957872191206576 1105 30-31 0.12403235238117105 1105 32-33 0.16706396724442385 1105 34-35 0.09954567824308214 1105 36-37 -0.012673981663105849 1105 38-39 -0.13530227720534782 1105 40-41 -0.12637121583225763 1105 42-43 -0.11626362665935375 1105 44-45 -0.03885807070012248 1105 46-47 -0.06188489342638803 1105 48-49 0.03582617407924715 1105 50-51 0.16239137811991355 1105 52-53 0.12997259448246012 1105 54-55 0.13830383897852272 1105 56-57 0.24744860066031293 1105 58-59 0.121532431461425 1105 60-61 -0.013293157646110387 1105 62-63 -0.056437622934261356 1105 64-65 -0.07405911725928149 1105 66-67 -0.1077210110372846 1105 68-69 0.1235228674494735 1105 70-71 -0.08204497526457288 1105 72-73 -0.1231838097396043 1105 74-75 0.001191789703035795 1105 76-77 0.10822130279736086 1105 78-79 0.12889971456726457 1105 80-81 0.17212013686539152 1105 82-83 0.2552384291503813 1105 84-85 0.18409446174903366 1105 86-87 0.32711091420182115 1105 88-89 0.23045083938522737 1105 90-91 0.20137676363740908 1105 92-93 0.19128307218181106 1105 94-95 0.22663090020472154 1105 96-97 0.326973977821023 1105 98-99 0.18918680038241575 1105 100-101 0.48145530436436346 1106 1 0.19419362295494835 1106 2 0.30540892654996554 1106 3 0.14454954331340275 1106 4 0.10762867068118709 1106 5 0.18758081346961575 1106 6 0.15219943792178725 1106 7 0.1118018015955684 1106 8 0.24186097920294003 1106 9 0.23489964932904428 1106 10-11 0.31725702522628296 1106 12-13 0.2647581672024515 1106 14-15 0.15806613089189625 1106 16-17 0.29992357330898756 1106 18-19 0.13945747339452197 1106 20-21 0.19458140826308323 1106 22-23 0.3185512040790357 1106 24-25 0.3626139205668082 1106 26-27 0.5329644271971574 1106 28-29 0.6918033038468963 1106 30-31 0.43873220683806124 1106 32-33 0.5388857808898351 1106 34-35 0.6440217230347187 1106 36-37 0.6897089155359382 1106 38-39 0.567036065979373 1106 40-41 0.4302606491987788 1106 42-43 0.3418073330341187 1106 44-45 0.6960715339572587 1106 46-47 0.5418851187517006 1106 48-49 0.3538044614229392 1106 50-51 0.565003728492691 1106 52-53 0.7124383621481272 1106 54-55 0.4694998172361622 1106 56-57 0.2535079466073853 1106 58-59 0.3482306819737957 1106 60-61 0.2843944890108432 1106 62-63 0.5907799788716588 1106 64-65 0.6865373124790466 1106 66-67 0.6023920076456974 1106 68-69 0.5392303851968236 1106 70-71 0.5692493188513268 1106 72-73 0.753616915005697 1106 74-75 1.0259898115047967 1106 76-77 0.9061205810223285 1106 78-79 1.1525393319967954 1106 80-81 1.1020997905321899 1106 82-83 1.0069686100396815 1106 84-85 0.877811603163476 1106 86-87 0.8043957925693626 1106 88-89 1.171732361350358 1106 90-91 1.1252302403356573 1106 92-93 1.1921147471157205 1106 94-95 1.1835174656842717 1106 96-97 1.3330593136097768 1106 98-99 1.6348717440958431 1106 100-101 1.8054817991515435 1107 1 0.10856781278643268 1107 2 0.16900535460700183 1107 3 0.06563552919846671 1107 4 0.07556040048098822 1107 5 0.16854725448934715 1107 6 0.2197854958218315 1107 7 0.3107863903900423 1107 8 0.21688618444563446 1107 9 0.13443875346270318 1107 10-11 0.23719437219445183 1107 12-13 0.16042286545968665 1107 14-15 0.3114148274207693 1107 16-17 0.32105996967942474 1107 18-19 0.2051819440844227 1107 20-21 0.2103310841956727 1107 22-23 0.17251562867934922 1107 24-25 0.28472972065322466 1107 26-27 0.5658788734019566 1107 28-29 0.3136562492595445 1107 30-31 0.5256254806390146 1107 32-33 0.32222511547142574 1107 34-35 0.3734866517398885 1107 36-37 0.34879360515137847 1107 38-39 0.5559061509570498 1107 40-41 0.644912096699862 1107 42-43 0.7781640952406548 1107 44-45 0.6582456661767608 1107 46-47 0.6350977069700505 1107 48-49 0.7054356006999072 1107 50-51 0.49135400935110596 1107 52-53 0.7021222163898102 1107 54-55 0.5703315902449475 1107 56-57 0.3913734226263941 1107 58-59 0.2212754752908097 1107 60-61 0.23270933849953934 1107 62-63 0.17097225886590195 1107 64-65 0.46987304631167603 1107 66-67 0.38427663964915837 1107 68-69 0.5613137785426403 1107 70-71 0.8599269798048041 1107 72-73 0.5598534121971106 1107 74-75 0.6166458679645466 1107 76-77 0.7511623497102136 1107 78-79 0.8128986867425816 1107 80-81 0.6417332342884912 1107 82-83 0.8317489643533804 1107 84-85 0.7858160680921813 1107 86-87 0.9641970312270054 1107 88-89 1.0620931563957257 1107 90-91 0.9697895086634709 1107 92-93 1.0452401972093384 1107 94-95 1.263713346427469 1107 96-97 0.9581097240951593 1107 98-99 1.1223231339849384 1107 100-101 1.2878021217786504 1108 1 0.08115302551972547 1108 2 0.11236404988831339 1108 3 0.12558980945367892 1108 4 -0.018618861861639857 1108 5 0.08765742542660604 1108 6 0.12019151445092291 1108 7 0.06912341997139748 1108 8 0.14244488655078413 1108 9 0.2415048423773669 1108 10-11 0.3185942834845008 1108 12-13 0.19931706178163466 1108 14-15 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-1.369632936632783 2316 50-51 -1.0995533711789207 2316 52-53 -1.2408439718593982 2316 54-55 -1.109448552742741 2316 56-57 -1.1908821275536852 2316 58-59 -1.2486245408356211 2316 60-61 -1.3281356864964309 2316 62-63 -1.3056256016942243 2316 64-65 -1.7619685921337336 2316 66-67 -1.4329840172341406 2316 68-69 -1.0577906669768993 2316 70-71 -1.1886968980643573 2316 72-73 -1.17270047696055 2316 74-75 -1.2317280502206955 2316 76-77 -1.5026542390120312 2316 78-79 -1.211586611327622 2316 80-81 -1.357772221820742 2316 82-83 -1.3731884113387132 2316 84-85 -1.5839307469376536 2316 86-87 -1.4551842513618247 2316 88-89 -1.6291609424055409 2316 90-91 -1.8867067553799615 2316 92-93 -1.6521741983182636 2316 94-95 -1.7068720430258466 2316 96-97 -1.62651909921291 2316 98-99 -1.666608758720539 2316 100-101 -1.1479609271919458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 8.0 5 27.0 6 56.0 7 153.0 8 287.0 9 590.0 10 913.0 11 1481.0 12 2173.0 13 3066.0 14 3504.0 15 5061.0 16 6537.0 17 9308.0 18 8418.0 19 4758.0 20 3855.0 21 3890.0 22 4122.0 23 4336.0 24 4799.0 25 5396.0 26 5920.0 27 6678.0 28 8108.0 29 9669.0 30 11903.0 31 14146.0 32 17238.0 33 21896.0 34 29601.0 35 41978.0 36 61004.0 37 110559.0 38 130705.0 39 15151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.20964604652084 23.64008341865332 16.33753245608195 20.812738078743898 2 14.614622697371962 23.966172496581724 40.59892552996781 20.82027927607851 3 17.200851250139063 28.793033529637647 32.91937168265452 21.08674353756877 4 11.509820598674319 20.388015029660973 40.50472099307731 27.5974433785874 5 10.787047504207802 40.08752947256225 36.34123933694361 12.784183686286333 6 25.67279983061127 41.419491905587314 18.91932144023485 13.988386823566564 7 23.420862805895588 36.318809685303016 23.897448043954938 16.362879464846454 8 23.00654228079053 36.31234994563052 22.3314994850152 18.349608288563747 9 24.687151218916988 18.100908311172837 24.033820325929753 33.17812014398042 10-11 21.69243385047138 29.248086302122022 29.81358267928469 19.245897168121903 12-13 23.273455135313604 28.024324508611194 29.63881083370118 19.063409522374027 14-15 20.84491473872529 29.7383574352174 28.69311503767359 20.723612788383726 16-17 17.93194280956619 32.41517972495765 30.06920918147627 19.583668283999884 18-19 17.643612573524397 30.761549476222775 33.72118686950249 17.873651080750335 20-21 18.679234449073924 29.603551421322166 33.736169876798414 17.9810442528055 22-23 18.70202297514077 29.65971526903021 33.221274793736924 18.416986962092093 24-25 18.441945280572764 30.07953561607579 33.612567793682906 17.865951309668535 26-27 18.143948263966458 30.073363891901977 34.08653117180357 17.696156672327987 28-29 17.772448492547973 29.924474876995788 34.256178920433946 18.046897710022286 30-31 18.45529680709423 29.856378454614944 33.77833762188273 17.909987116408097 32-33 17.7809717601714 30.59539061686925 33.57700906875675 18.0466285542026 34-35 18.358731881944973 30.104480223226105 33.61140893532454 17.925378959504382 36-37 18.50697276668219 29.738237466203998 33.334858808316895 18.41993095879692 38-39 17.840774503822274 30.268097476940746 33.47530775580519 18.415820263431794 40-41 17.90034277920353 30.032752463164698 33.93200050250355 18.13490425512823 42-43 18.047698559317244 29.879744217574768 33.25449669415481 18.818060528953172 44-45 18.08964388635083 30.070124566207422 32.873402548744465 18.966828998697277 46-47 18.675499778394645 30.085304604187712 32.17036876207839 19.068826855339253 48-49 18.586465409888426 30.114050292662093 31.959831185469895 19.339653111979587 50-51 18.197355813227396 30.434255999483224 31.696596793815875 19.67179139347351 52-53 18.235217065196718 30.826146872947685 31.89379829103998 19.044837770815615 54-55 18.530480999393504 30.267648547096886 31.455792053802455 19.74607839970716 56-57 18.607459563824023 30.699284763268487 30.919364505165998 19.773891167741496 58-59 18.28313550274092 30.236768677271463 31.564521483235985 19.915574336751632 60-61 18.47503384716424 30.815140202176806 30.98776298282116 19.722062967837793 62-63 18.03983211583218 30.59086039738807 31.480524467556332 19.888783019223418 64-65 18.18138590125929 30.35126628841302 31.148146951900063 20.319200858427628 66-67 18.096422380844718 30.499660863667195 30.61961464064109 20.784302114846994 68-69 18.0291819525154 30.088471756058038 30.900068455481062 20.9822778359455 70-71 18.225258299868294 30.078521724463393 31.001636467383697 20.694583508284616 72-73 18.40245254782899 29.443493427214882 30.813406830815833 21.340647194140296 74-75 17.974315357313323 30.31762181095213 30.060757440364043 21.647305391370505 76-77 17.916068673047665 29.84599641023981 30.771402776129175 21.466532140583354 78-79 17.805464939762928 29.85736535928713 30.587585098098323 21.749584602851616 80-81 18.043356164014853 29.7945776981864 30.1797445140869 21.982321623711847 82-83 18.0564078823179 29.79016612297191 29.98925171093383 22.164174283776365 84-85 17.735663863857397 29.439366371313014 30.047748242412496 22.77722152241709 86-87 17.67878151071836 29.630358056955036 29.9617425977315 22.729117834595108 88-89 18.040268142180896 29.30045716693345 29.835589158355496 22.823685532530156 90-91 17.895830482350313 28.834872263956612 30.030664573144822 23.238632680548253 92-93 18.068340456990693 29.175234793593376 29.742974135920104 23.01345061349583 94-95 17.840650639918536 28.560867400266467 29.900546835397613 23.697935124417388 96-97 17.92057391198246 28.53428506831175 29.51266647287448 24.032474546831317 98-99 17.813629332960105 28.809003441605746 29.197933601053656 24.179433624380493 100-101 17.817429485534646 28.515854203413976 29.755947008507132 23.910769302544242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1389.0 1 851.0 2 339.5 3 353.5 4 293.0 5 309.5 6 553.0 7 962.5 8 1305.0 9 1346.0 10 1160.0 11 1073.0 12 1091.5 13 1126.5 14 1145.0 15 1134.0 16 1237.5 17 1400.0 18 1545.5 19 1762.5 20 2043.5 21 2304.5 22 2638.5 23 3081.5 24 3552.5 25 4290.5 26 5724.5 27 7656.0 28 8784.5 29 9788.0 30 11817.5 31 13884.0 32 16698.0 33 20024.0 34 22831.0 35 25276.0 36 27863.0 37 29545.5 38 28190.0 39 25785.0 40 24017.0 41 22459.5 42 20801.5 43 20018.5 44 19903.0 45 18809.5 46 17156.0 47 16181.5 48 17135.5 49 15634.0 50 11854.0 51 9291.0 52 7107.0 53 5634.5 54 4763.0 55 4245.0 56 3674.5 57 3204.0 58 2888.0 59 2615.0 60 2250.5 61 1939.5 62 1864.5 63 1786.0 64 1610.0 65 1432.5 66 1285.5 67 1113.5 68 1007.0 69 907.5 70 750.0 71 626.5 72 491.0 73 381.5 74 301.0 75 217.0 76 148.5 77 119.5 78 88.0 79 51.0 80 30.5 81 18.5 82 12.0 83 7.5 84 1.0 85 0.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27758936870399675 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0019738093443723106 16-17 3.588744262495111E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.971860656237777E-5 26-27 0.0013457790984356664 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0027812768034337102 36-37 0.016956816640289397 38-39 0.006818614098740709 40-41 0.015790474754978485 42-43 0.004934523360930777 44-45 7.177488524990222E-4 46-47 1.7943721312475554E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.383116393742665E-4 60-61 0.0024224023771841994 62-63 0.0016149349181227997 64-65 0.0 66-67 0.0 68-69 2.6915581968713325E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22824413509468902 78-79 0.0 80-81 0.0012560604918732887 82-83 0.0 84-85 0.0 86-87 0.09743440672674225 88-89 0.34469888641265534 90-91 0.3470315701832772 92-93 0.0 94-95 8.971860656237777E-5 96-97 0.0 98-99 0.0 100-101 1.7943721312475554E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.31224215094679 #Duplication Level Percentage of deduplicated Percentage of total 1 77.09574004831991 33.39189361779278 2 11.681113680978617 10.118704486865667 3 4.036252857029224 5.244574833783016 4 1.9219157481977986 3.3296992111864454 5 1.1212796860542256 2.4282568640659106 6 0.6701430896127057 1.7415239863853489 7 0.5042797787066462 1.5289041521017686 8 0.3529615374416459 1.2230044463714431 9 0.27249522838078744 1.0622141384945594 >10 1.926326778938101 16.853013348772773 >50 0.25860478590475766 7.793394406580095 >100 0.15309403323223555 11.642331597272092 >500 0.002896373601690943 0.8449848143762378 >1k 0.002896373601690943 2.797500095951871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4610 0.8272055525051228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3442 0.6176228875754084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 0.43513524182753216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1691 0.3034283273939616 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1241 0.2226815814878216 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1106 0.19845755771597962 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.1964837483716073 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.16562054771414933 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 782 0.14031990066355882 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 698 0.12524717476107936 RNA PCR Primer, Index 17 (95% over 21bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 692 0.12417055148233082 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 565 0.10138202541548687 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.794372131247555E-4 0.0 14-15 0.0 0.0 1.794372131247555E-4 1.794372131247555E-4 0.0 16-17 0.0 0.0 1.794372131247555E-4 1.794372131247555E-4 0.0 18-19 0.0 0.0 1.794372131247555E-4 2.6915581968713325E-4 0.0 20-21 0.0 0.0 1.794372131247555E-4 6.280302459366443E-4 0.0 22-23 0.0 0.0 1.794372131247555E-4 0.0017046535246851772 0.0 24-25 0.0 0.0 1.794372131247555E-4 0.00601114663967931 0.0 26-27 0.0 0.0 1.794372131247555E-4 0.013547509590919042 0.0 28-29 0.0 1.794372131247555E-4 1.794372131247555E-4 0.03095291926402033 0.0 30-31 0.0 1.794372131247555E-4 1.794372131247555E-4 0.07796546910270627 0.0 32-33 0.0 1.794372131247555E-4 1.794372131247555E-4 0.16920929197664447 0.0 34-35 0.0 1.794372131247555E-4 1.794372131247555E-4 0.3213720487064371 0.0 36-37 0.0 1.794372131247555E-4 1.794372131247555E-4 0.5425284138826983 0.0 38-39 0.0 1.794372131247555E-4 1.794372131247555E-4 0.8177850988160733 0.0 40-41 0.0 1.794372131247555E-4 1.794372131247555E-4 1.105064077028807 0.0 42-43 0.0 1.794372131247555E-4 1.794372131247555E-4 1.3942271459793503 0.0 44-45 0.0 1.794372131247555E-4 1.794372131247555E-4 1.752742697802612 0.0 46-47 0.0 1.794372131247555E-4 1.794372131247555E-4 2.179982702252655 0.0 48-49 0.0 1.794372131247555E-4 1.794372131247555E-4 2.600852685636769 0.0 50-51 0.0 1.794372131247555E-4 1.794372131247555E-4 3.023427322545568 0.0 52-53 0.0 1.794372131247555E-4 1.794372131247555E-4 3.4235723078137728 0.0 54-55 0.0 1.794372131247555E-4 1.794372131247555E-4 3.8746774616094077 0.0 56-57 0.0 1.794372131247555E-4 1.794372131247555E-4 4.422050680246475 0.0 58-59 0.0 1.794372131247555E-4 1.794372131247555E-4 4.995532013393193 0.0 60-61 0.0 1.794372131247555E-4 1.794372131247555E-4 5.5919813098198805 1.794372131247555E-4 62-63 0.0 1.794372131247555E-4 1.794372131247555E-4 6.178382122311582 1.794372131247555E-4 64-65 0.0 1.794372131247555E-4 1.794372131247555E-4 6.806591805461351 1.794372131247555E-4 66-67 0.0 1.794372131247555E-4 1.794372131247555E-4 7.5404002885350385 1.794372131247555E-4 68-69 0.0 1.794372131247555E-4 1.794372131247555E-4 8.348136903416126 1.794372131247555E-4 70-71 0.0 1.794372131247555E-4 1.794372131247555E-4 9.229442775678361 1.794372131247555E-4 72-73 0.0 1.794372131247555E-4 1.794372131247555E-4 10.063018349249415 1.794372131247555E-4 74-75 0.0 1.794372131247555E-4 1.794372131247555E-4 10.946387749462586 3.58874426249511E-4 76-77 0.0 1.794372131247555E-4 1.794372131247555E-4 11.901980627958471 3.58874426249511E-4 78-79 0.0 1.794372131247555E-4 1.794372131247555E-4 12.963082587771712 3.58874426249511E-4 80-81 0.0 1.794372131247555E-4 1.794372131247555E-4 14.089858567588616 3.58874426249511E-4 82-83 0.0 1.794372131247555E-4 1.794372131247555E-4 15.17635089305901 3.58874426249511E-4 84-85 0.0 1.794372131247555E-4 1.794372131247555E-4 16.28608033762906 3.58874426249511E-4 86-87 0.0 1.794372131247555E-4 1.794372131247555E-4 17.488399384171487 3.58874426249511E-4 88-89 0.0 1.794372131247555E-4 1.794372131247555E-4 18.73450111071635 3.58874426249511E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 45 9.659665E-4 42.187645 4 ACGCCGA 70 2.0618773E-4 33.90079 6 AGGTAAC 60 0.0039044956 31.740412 1 ACACGGA 60 0.0039650896 31.640738 6 TACGCCG 75 3.0912456E-4 31.640734 5 CATGGGG 795 0.0 29.252756 4 CGGAGGT 65 0.0058623813 29.209454 9 TACACGG 65 0.0058649527 29.206835 5 CGCCGAA 105 6.93814E-5 27.120632 7 GTACATG 2460 0.0 25.740702 1 GTGCTAG 115 1.2605233E-4 24.840322 1 ACAGTCC 115 1.2877377E-4 24.762314 8 GTATCAA 5900 0.0 24.612268 1 GGTACTC 155 1.4185407E-6 24.496054 8 ACATGGG 2330 0.0 24.443487 3 ATGGGGA 525 0.0 24.408567 5 GGTATCA 4370 0.0 24.186632 1 TACATGG 2535 0.0 23.964582 2 GTGTAGC 100 0.0016303494 23.805311 1 TGTTCGC 100 0.0016604785 23.730553 8 >>END_MODULE