##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628185 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970543709257623 33.0 31.0 34.0 30.0 34.0 2 31.928259987105708 33.0 31.0 34.0 30.0 34.0 3 31.956638569848053 34.0 31.0 34.0 30.0 34.0 4 35.47942883067886 37.0 35.0 37.0 33.0 37.0 5 35.546942381623246 37.0 35.0 37.0 33.0 37.0 6 35.71161680078321 37.0 35.0 37.0 35.0 37.0 7 35.67526126857534 37.0 35.0 37.0 35.0 37.0 8 35.69209866520213 37.0 35.0 37.0 35.0 37.0 9 37.26771572068738 39.0 37.0 39.0 34.0 39.0 10-11 37.18282671506006 39.0 37.0 39.0 34.0 39.0 12-13 37.10123132516695 39.0 37.0 39.0 33.5 39.0 14-15 38.11703717853817 40.0 38.0 41.0 33.0 41.0 16-17 38.11084712306088 40.0 38.0 41.0 33.0 41.0 18-19 38.18871669969833 40.0 38.0 41.0 33.5 41.0 20-21 38.185487555417595 40.0 38.0 41.0 34.0 41.0 22-23 38.11425137499303 40.0 38.0 41.0 34.0 41.0 24-25 37.98193923764496 40.0 38.0 41.0 33.0 41.0 26-27 37.74163502789783 40.0 37.0 41.0 33.0 41.0 28-29 37.49488765252274 40.0 36.5 41.0 33.0 41.0 30-31 37.11096651464139 39.0 35.5 41.0 33.0 41.0 32-33 36.629235018346506 39.0 35.0 41.0 32.0 41.0 34-35 36.21668377945987 38.5 35.0 41.0 31.0 41.0 36-37 35.81060276829278 38.0 35.0 41.0 30.0 41.0 38-39 35.03363499606007 38.0 34.5 40.0 27.5 41.0 40-41 34.79689024729976 38.0 35.0 40.0 26.5 41.0 42-43 34.60111432141805 38.0 35.0 40.0 24.0 41.0 44-45 34.118676822910444 38.0 35.0 41.0 19.5 41.0 46-47 33.316758598183654 38.0 34.0 40.0 5.0 41.0 48-49 32.69298614261722 38.0 33.0 40.0 2.0 41.0 50-51 31.407543160056353 36.5 31.5 39.0 2.0 40.5 52-53 31.377008365370074 36.5 31.5 39.5 2.0 40.5 54-55 31.73738309574409 37.0 33.0 40.0 2.0 41.0 56-57 31.60370352682729 37.0 33.0 40.0 2.0 41.0 58-59 31.359591521605896 36.5 32.0 40.0 2.0 41.0 60-61 30.988292461615607 36.0 31.5 40.0 2.0 41.0 62-63 30.507777963498015 35.5 31.0 39.5 2.0 41.0 64-65 30.029686318520817 35.0 30.0 39.0 2.0 41.0 66-67 29.598900801515477 35.0 29.0 39.0 2.0 40.5 68-69 29.15329401370615 35.0 29.0 38.0 2.0 40.0 70-71 28.647066548866974 35.0 27.5 37.0 2.0 39.5 72-73 28.104294117178856 35.0 26.0 37.0 2.0 39.0 74-75 27.59436471740013 35.0 26.0 36.0 2.0 39.0 76-77 27.02143874813948 34.0 24.5 35.5 2.0 37.5 78-79 26.54785692112992 34.0 21.5 35.0 2.0 37.0 80-81 26.077869576637454 34.0 19.0 35.0 2.0 37.0 82-83 25.531470028733573 34.0 6.0 35.0 2.0 36.0 84-85 24.994488088699985 33.5 2.0 35.0 2.0 36.0 86-87 24.407384767226215 33.0 2.0 35.0 2.0 35.0 88-89 23.79992756910783 33.0 2.0 35.0 2.0 35.0 90-91 23.17086925030047 32.0 2.0 35.0 2.0 35.0 92-93 22.577630793476445 32.0 2.0 35.0 2.0 35.0 94-95 21.964059154548423 31.0 2.0 35.0 2.0 35.0 96-97 21.226110142712734 31.0 2.0 35.0 2.0 35.0 98-99 20.404953158703247 30.0 2.0 34.0 2.0 35.0 100-101 18.338741771930245 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3067817452982986 1101 2 0.130283392092867 1101 3 0.4267756740426627 1101 4 0.38128974868531884 1101 5 0.22766081734423693 1101 6 0.4727458773696753 1101 7 0.5104135560886078 1101 8 0.22002301293357363 1101 9 0.32251332910639974 1101 10-11 0.39540898762680854 1101 12-13 0.5587974319709161 1101 14-15 0.4320899435702614 1101 16-17 0.5034627702347834 1101 18-19 0.22102397515403283 1101 20-21 0.2759613628693032 1101 22-23 0.27473634900399446 1101 24-25 0.26212561234188314 1101 26-27 0.09796746771426257 1101 28-29 0.06757510511963005 1101 30-31 0.018821657485993626 1101 32-33 0.26254648529313585 1101 34-35 0.027133147019398507 1101 36-37 -0.08456895812757637 1101 38-39 0.43113417038104984 1101 40-41 0.3426344051096635 1101 42-43 0.2784601125728372 1101 44-45 0.24703774919742472 1101 46-47 0.30126926559435674 1101 48-49 0.33423887658742757 1101 50-51 0.5058210760385613 1101 52-53 0.555540607066181 1101 54-55 0.09854078137903954 1101 56-57 -0.0784735998428836 1101 58-59 -0.12845921859911158 1101 60-61 -0.08201396002752048 1101 62-63 0.20020670408959518 1101 64-65 0.28182088595892196 1101 66-67 0.2141427333099628 1101 68-69 0.19683835225066915 1101 70-71 0.1914906869996038 1101 72-73 -0.050153231884628724 1101 74-75 0.011288303648452569 1101 76-77 -0.034864100622481686 1101 78-79 0.020265942425314876 1101 80-81 -0.11305298027457056 1101 82-83 -0.049655359059656234 1101 84-85 -0.020460167281338926 1101 86-87 0.22181247251298686 1101 88-89 -0.1557443771000635 1101 90-91 -0.06631570394064212 1101 92-93 -0.14244261149890036 1101 94-95 0.08211970953705006 1101 96-97 0.02390853848688579 1101 98-99 0.015308684946287343 1101 100-101 -0.1901827482359515 1102 1 0.2632730009580406 1102 2 0.4566546710795798 1102 3 0.3562312250114523 1102 4 0.4248869520690377 1102 5 0.271015577423654 1102 6 0.4435478405051896 1102 7 0.36311614367944145 1102 8 0.29128496309509444 1102 9 0.5309785319397093 1102 10-11 0.5503450022520369 1102 12-13 0.507378155762467 1102 14-15 0.7654494869905477 1102 16-17 0.7515117568872967 1102 18-19 0.7556737101045243 1102 20-21 0.8377172421194317 1102 22-23 0.793365168305094 1102 24-25 0.5109297771247086 1102 26-27 0.5887873847593426 1102 28-29 0.32122158346682284 1102 30-31 0.5898247830388641 1102 32-33 0.5088099097720473 1102 34-35 0.6814057188186595 1102 36-37 0.4920349353808362 1102 38-39 0.7547189894986914 1102 40-41 0.16723977948799984 1102 42-43 0.08407464082403493 1102 44-45 0.39077222791155464 1102 46-47 0.44608654852569174 1102 48-49 0.30867093676579316 1102 50-51 0.2497468883874454 1102 52-53 0.18645507699993757 1102 54-55 0.25946743900322744 1102 56-57 0.12960008981658078 1102 58-59 0.06672566080172615 1102 60-61 -0.15340532249336647 1102 62-63 -0.20775821370902037 1102 64-65 -0.36644629966746933 1102 66-67 -0.43476823757718464 1102 68-69 -0.3371299204390752 1102 70-71 -0.17672499580592316 1102 72-73 -0.39566770312888977 1102 74-75 -0.5238528704323926 1102 76-77 -0.6412115284300768 1102 78-79 -0.5687959330374532 1102 80-81 -0.32588216258538694 1102 82-83 -0.4699783328561189 1102 84-85 -0.27954687680149704 1102 86-87 -0.4005165558135637 1102 88-89 -0.5385967553378279 1102 90-91 -0.5062045076467498 1102 92-93 -0.3960940042373977 1102 94-95 -0.5828367489560016 1102 96-97 -0.7106225958201513 1102 98-99 -0.7734330998295853 1102 100-101 -0.732154926228656 1103 1 0.07163929462634755 1103 2 -0.0037598009919150854 1103 3 0.10215507504358712 1103 4 -0.07291348386865337 1103 5 -0.12205380822220491 1103 6 0.005556334002562835 1103 7 0.07269749773637812 1103 8 0.113892109028221 1103 9 0.13331126238048085 1103 10-11 0.29387550132782536 1103 12-13 0.14233830522682211 1103 14-15 0.28705755382549114 1103 16-17 0.281404627517162 1103 18-19 0.25622691371658135 1103 20-21 0.1817320428004976 1103 22-23 0.33575646545163096 1103 24-25 0.3161507604358391 1103 26-27 0.26848249337938057 1103 28-29 0.2293616267955798 1103 30-31 0.248998356469194 1103 32-33 0.2782598200006774 1103 34-35 0.20659801504428543 1103 36-37 0.03928084266789256 1103 38-39 0.37469626508684684 1103 40-41 0.2373111328161599 1103 42-43 0.0810651469225192 1103 44-45 -0.09101014775013283 1103 46-47 -0.15955215320149563 1103 48-49 0.029601246361060873 1103 50-51 0.04452656759560014 1103 52-53 0.10262485419469769 1103 54-55 0.01590215766440295 1103 56-57 0.13684639461520476 1103 58-59 0.22215765650859964 1103 60-61 0.3387587305346784 1103 62-63 0.21303926076742385 1103 64-65 0.09939751803372232 1103 66-67 0.156346384886195 1103 68-69 0.39264170915963703 1103 70-71 0.18028015196900427 1103 72-73 0.2552210665065253 1103 74-75 0.3520970166080666 1103 76-77 0.5024491556873514 1103 78-79 0.9198087350735058 1103 80-81 0.9874430880125367 1103 82-83 0.7276495168049983 1103 84-85 0.4879287259254319 1103 86-87 0.4287621594290165 1103 88-89 0.18743992173039103 1103 90-91 0.07008955816792195 1103 92-93 0.17132395931079003 1103 94-95 0.4048006409829803 1103 96-97 0.3951419845732538 1103 98-99 0.14154083003612428 1103 100-101 -0.6485052399315414 1104 1 0.03553068213412658 1104 2 0.10332057106616688 1104 3 0.1263230349514579 1104 4 -0.15937118911509884 1104 5 0.0365552243674756 1104 6 -0.08503974311086182 1104 7 -0.12914038747651801 1104 8 0.11123972897016188 1104 9 0.10772320830487558 1104 10-11 0.2388476760488274 1104 12-13 0.28555364883359147 1104 14-15 0.020763538319776842 1104 16-17 0.05511706998027677 1104 18-19 0.15518111815083557 1104 20-21 0.41223375449173005 1104 22-23 0.26250629494148114 1104 24-25 0.40645524048697723 1104 26-27 0.4094704967138014 1104 28-29 0.40495742384402433 1104 30-31 0.14646327421245786 1104 32-33 0.3795955651876781 1104 34-35 0.31196175354926226 1104 36-37 0.2202324705043779 1104 38-39 0.732518809328873 1104 40-41 0.6637034676025308 1104 42-43 0.138849141044453 1104 44-45 0.2923065464287973 1104 46-47 0.3970094396762889 1104 48-49 0.291257715367756 1104 50-51 0.3117760988986298 1104 52-53 0.33372952930285393 1104 54-55 0.49542281822236234 1104 56-57 0.42395253265098987 1104 58-59 0.27095485981270784 1104 60-61 0.4604897956712044 1104 62-63 0.4847599470254593 1104 64-65 0.5690088207971407 1104 66-67 0.3127017181470393 1104 68-69 0.3810288973402365 1104 70-71 0.41487410717837747 1104 72-73 -0.03883562523419215 1104 74-75 0.013877373180203989 1104 76-77 0.22282734225016299 1104 78-79 0.26086691939579865 1104 80-81 0.2038937751601928 1104 82-83 0.14811435876598367 1104 84-85 0.3519291740293937 1104 86-87 0.07700055073580003 1104 88-89 0.1442324858405577 1104 90-91 0.1273463711603 1104 92-93 0.15985447486098536 1104 94-95 0.5886600022371447 1104 96-97 0.773842548986547 1104 98-99 0.6507566151570963 1104 100-101 0.46143179487230057 1105 1 0.15140731523836948 1105 2 0.3096069442355933 1105 3 0.3560425813593149 1105 4 0.34065756138803494 1105 5 0.10095332501844467 1105 6 0.16925997029201767 1105 7 0.2585132385671969 1105 8 0.3404266344778506 1105 9 0.4676885866463678 1105 10-11 0.3877813350194401 1105 12-13 0.5683572163170751 1105 14-15 0.390048619229745 1105 16-17 0.33696733130722123 1105 18-19 0.3503728234793613 1105 20-21 0.5762099793388202 1105 22-23 0.6447139704179037 1105 24-25 0.6354817484508715 1105 26-27 0.683297854482035 1105 28-29 0.4789748375698224 1105 30-31 0.7146125589302059 1105 32-33 0.667459221222714 1105 34-35 0.4980440889374975 1105 36-37 0.41709624456778016 1105 38-39 0.8288087838771148 1105 40-41 0.7846704529458322 1105 42-43 0.7929503246572764 1105 44-45 0.6675596265155903 1105 46-47 0.7166015456960366 1105 48-49 0.8548886315403621 1105 50-51 0.7696959546013638 1105 52-53 0.6611296666999955 1105 54-55 0.7563548187428957 1105 56-57 0.8155555699146682 1105 58-59 1.0713945911922202 1105 60-61 0.9596393860521921 1105 62-63 0.8350167344139763 1105 64-65 0.6805259697156671 1105 66-67 0.6528095779293253 1105 68-69 0.5992028469633013 1105 70-71 0.6710395769162041 1105 72-73 0.6485692332982005 1105 74-75 0.7852397536008091 1105 76-77 0.9951778320679452 1105 78-79 1.2400137255175352 1105 80-81 1.104979938904897 1105 82-83 0.8397273830465402 1105 84-85 0.7447881543247057 1105 86-87 0.7231393581649286 1105 88-89 0.6767871344942797 1105 90-91 0.9624334851847927 1105 92-93 0.9505387065718836 1105 94-95 1.047327371021364 1105 96-97 1.1107318656022223 1105 98-99 1.2817923293347242 1105 100-101 1.006196328251633 1106 1 0.2024488077088371 1106 2 0.28523695577151287 1106 3 0.33223663426582206 1106 4 0.3784086519152581 1106 5 0.25163740232896004 1106 6 0.3044031489344121 1106 7 0.32783930833259944 1106 8 0.29116229748155575 1106 9 0.441776696144089 1106 10-11 0.2836505114340042 1106 12-13 0.3445036516606521 1106 14-15 0.5420095841656405 1106 16-17 0.4091221110618193 1106 18-19 0.4705150526048669 1106 20-21 0.36412475523867016 1106 22-23 0.5330507853131294 1106 24-25 0.3331229784578724 1106 26-27 0.4494063374941888 1106 28-29 0.5207252365086248 1106 30-31 0.31116141936823283 1106 32-33 0.42836520204530615 1106 34-35 0.10679211550129963 1106 36-37 0.3920190786064097 1106 38-39 0.785533489172586 1106 40-41 0.5883089449429661 1106 42-43 0.5831666160994331 1106 44-45 0.6223753777251559 1106 46-47 1.0711157190069613 1106 48-49 0.9260768881423402 1106 50-51 0.8272281744017711 1106 52-53 0.8572729275393662 1106 54-55 1.0495117781347538 1106 56-57 1.067218641644292 1106 58-59 0.8571963377364078 1106 60-61 1.1719712011033074 1106 62-63 0.9389363967827791 1106 64-65 1.1232049111318965 1106 66-67 1.14595321574107 1106 68-69 1.048162771682204 1106 70-71 1.0928192266436803 1106 72-73 0.7910293606751821 1106 74-75 0.7064383556852114 1106 76-77 1.1327217295087841 1106 78-79 0.9457145114907632 1106 80-81 0.7561302715192433 1106 82-83 0.6754028580175593 1106 84-85 0.729401729298278 1106 86-87 0.8623498703088224 1106 88-89 0.8115731814145839 1106 90-91 0.628355182637538 1106 92-93 0.48975517091114895 1106 94-95 0.5596767592207108 1106 96-97 0.7051719482527048 1106 98-99 0.6535494457511639 1106 100-101 0.6953287532610553 1107 1 0.37376816430152715 1107 2 0.3689935714075041 1107 3 0.46378157140874166 1107 4 0.40047488887866933 1107 5 0.4138362662292465 1107 6 0.28724962792694697 1107 7 0.34224138052602626 1107 8 0.23548302843248337 1107 9 0.4046007154452127 1107 10-11 0.49919933940424954 1107 12-13 0.5403797572266669 1107 14-15 0.5937379147985311 1107 16-17 0.47605991520347857 1107 18-19 0.5661231173281323 1107 20-21 0.5128942537755066 1107 22-23 0.33153926447507587 1107 24-25 0.49464969100933587 1107 26-27 0.3516738919835305 1107 28-29 0.4029006570813749 1107 30-31 0.2922428179894965 1107 32-33 0.40137395367445805 1107 34-35 0.377435495493593 1107 36-37 0.48739626207366626 1107 38-39 0.4898101204374541 1107 40-41 0.07359812030069435 1107 42-43 0.12150520004593801 1107 44-45 0.06143228927168565 1107 46-47 -0.07966886741503743 1107 48-49 -0.16555018261662013 1107 50-51 -0.1617018207620191 1107 52-53 -0.17720488463531225 1107 54-55 -0.08714396834943372 1107 56-57 -0.0747200505021901 1107 58-59 -0.08174854858532044 1107 60-61 0.012684993047873405 1107 62-63 -0.1269385338845339 1107 64-65 0.04347573894365908 1107 66-67 -0.0989667135464245 1107 68-69 0.03167320505588478 1107 70-71 0.18498593110747308 1107 72-73 0.05615344910406961 1107 74-75 0.12853847344831237 1107 76-77 0.27611948190024904 1107 78-79 0.3607543077687936 1107 80-81 0.2656770065304066 1107 82-83 0.4769665662164364 1107 84-85 0.25301036234462515 1107 86-87 0.17968864278559238 1107 88-89 0.15128967562615614 1107 90-91 0.35106132954359026 1107 92-93 0.3300468190207937 1107 94-95 0.1810000776811016 1107 96-97 0.2176928163662275 1107 98-99 0.6236933571801977 1107 100-101 0.6196803048104194 1108 1 -0.3064939099841304 1108 2 -0.020219059030228692 1108 3 -0.09732218457236641 1108 4 -0.1652495682606201 1108 5 -0.03963095363586433 1108 6 -0.16559993476134593 1108 7 0.07388367101775373 1108 8 0.02675399489656627 1108 9 -0.05513429138289894 1108 10-11 0.2462048748635013 1108 12-13 0.11589812014846501 1108 14-15 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#Quality Count 2 3008.0 3 495.0 4 433.0 5 539.0 6 610.0 7 823.0 8 1113.0 9 1460.0 10 1942.0 11 2868.0 12 4613.0 13 6955.0 14 7857.0 15 9792.0 16 12887.0 17 16776.0 18 13666.0 19 7824.0 20 7336.0 21 6920.0 22 7047.0 23 7530.0 24 8188.0 25 8815.0 26 9798.0 27 11162.0 28 12769.0 29 14777.0 30 16975.0 31 19738.0 32 23341.0 33 28555.0 34 37975.0 35 61274.0 36 70802.0 37 92831.0 38 77419.0 39 11271.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.38698883808843 18.91490638808715 13.884862455620548 17.81324231820388 2 14.385764607016599 24.229604552927462 39.70719790532169 21.67743293473425 3 22.559014372668805 31.7268730475384 29.23202778535241 16.482084794440375 4 12.283902866940469 17.02125284102237 38.22449060967343 32.47035368236373 5 11.676849207995023 41.137839174336804 30.91451450812742 16.270797109540748 6 25.384120164834638 36.03797698306716 19.89265988673196 18.685242965366243 7 23.71427296459091 38.748713077680705 21.41024374229042 16.12677021543797 8 24.94863567752291 29.58441521180909 22.31323549804664 23.153713612621356 9 29.256959775565665 18.690215268866908 22.82407887081477 29.22874608475265 10-11 23.06739715111067 28.194122920609622 26.21408506823389 22.52439486004582 12-13 29.335388042897108 26.388144092085835 27.03642939498949 17.24003847002756 14-15 24.999641386828856 26.215180653377256 31.254572317932094 17.530605641861793 16-17 18.15700322203348 30.27580096692874 31.77192719670339 19.795268614334386 18-19 17.41408673850379 29.042392190522186 37.31473888313756 16.22878218783646 20-21 19.42417425436967 27.2927724039852 35.41510886912268 17.867944472522442 22-23 18.12528997881062 27.905488033341786 34.32719335587794 19.642028631969655 24-25 18.319073097992934 28.172749527538336 35.71666653376606 17.791510840702674 26-27 19.473461405692063 26.7159780060159 36.76346900329495 17.047091584997087 28-29 17.495906285426376 27.79349183090687 37.60254216884759 17.10805971481917 30-31 17.361609865167015 30.1478260522991 36.08916593265126 16.401398149882624 32-33 16.135906323842203 29.96434846663329 35.61773137621934 18.282013833305164 34-35 17.228573299704657 28.648532951683002 35.866052874117685 18.25684087449465 36-37 18.38676894020868 28.5676933201027 35.60716852985648 17.438369209832143 38-39 19.154538246089704 27.559038124592345 36.06633116343313 17.22009246588482 40-41 17.241897430323334 28.96968369767457 36.44565891522433 17.34275995677777 42-43 16.36012614967683 30.465306840302343 35.01684793687719 18.15771907314363 44-45 16.90998062921862 30.252475710575105 33.611623428172614 19.225920232033662 46-47 17.004992744730433 30.42040589535623 32.39807645415189 20.176524905761443 48-49 17.759561312257173 30.117483942208743 32.41151123545644 19.711443510077647 50-51 18.197045579562754 30.97168387253585 32.09822056772972 18.733049980171675 52-53 17.011246107039913 31.25410285010655 32.27188952988634 19.462761512967205 54-55 15.882902198921405 30.500781046394316 33.03610400947809 20.580212745206197 56-57 17.47311892403774 31.177253275475586 30.70767853789323 20.64194926259345 58-59 17.282563618624653 29.585507091198348 31.681903280992678 21.45002600918432 60-61 17.49662354548889 29.102761624308236 32.29887233208179 21.101742498121087 62-63 19.002493805463985 28.601286160778894 31.948854200730953 20.447365833026172 64-65 17.97329052055741 29.331095683515933 32.69828426414609 19.99732953178057 66-67 17.225828067164937 29.348157234208095 32.28764274228306 21.13837195634391 68-69 17.17572055656452 30.25949712785006 31.945280805337568 20.619501510247854 70-71 17.162080899710205 30.14291471816885 31.03253324974251 21.662471132378435 72-73 17.332113689173045 29.191580617252953 30.946285016127767 22.53002067744623 74-75 17.363209063474066 29.66499754509587 31.407259799225624 21.56453359220444 76-77 17.79730527002461 28.534734580129605 32.12634884393942 21.541611305906375 78-79 17.56612014170429 28.19570027240987 32.9081192208822 21.330060365003646 80-81 17.175968814418777 29.669224700681244 31.977609942042584 21.177196542857395 82-83 16.996299573839448 28.99282697689812 31.291583828761958 22.719289620500476 84-85 17.650237665458487 27.795187214509664 31.334348054788563 23.220227065243286 86-87 18.291150371732353 27.503477496202734 31.39251738748101 22.8128547445839 88-89 18.922326402435516 26.541224025122638 31.778251776428977 22.75819779601287 90-91 18.57198832513694 26.585822238215183 32.096197673023866 22.745991763624005 92-93 18.618262484226282 27.133350073330785 31.726800042862923 22.521587399580007 94-95 18.886292648053697 26.852380518277908 31.38031516337925 22.88101167028914 96-97 19.213186015287835 25.966684712247616 30.77925723109352 24.040872041371028 98-99 20.025140073549107 25.469357336879384 30.6500995927532 23.85540299681831 100-101 21.01632746712801 24.718648393036023 31.811259180852502 22.453764958983466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4245.0 1 2973.5 2 2457.0 3 3117.5 4 2415.0 5 2229.0 6 3840.0 7 6422.0 8 8556.5 9 7895.5 10 5628.0 11 4451.0 12 4093.5 13 3981.5 14 3868.0 15 3743.0 16 3671.0 17 3627.0 18 3709.0 19 3917.5 20 4246.5 21 4622.0 22 4908.5 23 5200.0 24 5660.0 25 6195.5 26 6679.0 27 7218.0 28 7756.0 29 8351.0 30 8993.0 31 9527.5 32 10062.5 33 10616.5 34 11349.0 35 11961.0 36 12304.5 37 12992.0 38 13144.5 39 12753.0 40 12740.0 41 12935.5 42 13028.0 43 13379.5 44 13953.5 45 14488.0 46 18042.5 47 24815.5 48 42125.5 49 45170.0 50 30845.5 51 22360.5 52 14832.5 53 11257.5 54 9731.5 55 8798.5 56 8435.0 57 7943.5 58 7319.5 59 6892.0 60 6699.5 61 6588.0 62 5669.0 63 3542.0 64 1828.5 65 1355.0 66 1072.0 67 826.0 68 647.0 69 505.5 70 460.5 71 411.0 72 386.0 73 454.5 74 379.5 75 278.0 76 226.5 77 155.5 78 110.0 79 73.0 80 44.5 81 32.0 82 23.0 83 20.0 84 16.5 85 8.5 86 5.0 87 5.0 88 4.5 89 1.0 90 0.5 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2816049412195452 2 0.1706503657362083 3 0.17383414121636143 4 0.19261841654926493 5 0.2061494623399158 6 0.10299513678295405 7 0.11445672851150537 8 0.12798777430215622 9 0.1321266824263553 10-11 0.1189936085707236 12-13 0.10880552703423355 14-15 0.12225697843788055 16-17 0.10044811639883156 18-19 0.1263162921750758 20-21 0.15345797814338133 22-23 0.15664175362353447 24-25 0.18306709010880554 26-27 0.16133782245676034 28-29 0.1598255291036876 30-31 0.14764758789210186 32-33 0.15966634032967994 34-35 0.3393904661843247 36-37 0.45663299824096404 38-39 0.4229645725383446 40-41 0.4108662257137627 42-43 0.43577926884596097 44-45 0.4391222331001218 46-47 0.44214681980626724 48-49 0.4561554319189411 50-51 0.44843477637956974 52-53 0.45488192172687986 54-55 0.4364956183289954 56-57 0.39996179469423815 58-59 0.3891369580617175 60-61 0.3436089686955276 62-63 0.4513797686987114 64-65 0.4499470697326424 66-67 0.4598167737211172 68-69 0.4706416103536379 70-71 0.46435365378033544 72-73 0.4562350263059449 74-75 0.4626025772662512 76-77 0.4364956183289954 78-79 0.4229645725383446 80-81 0.42017876899321055 82-83 0.43243630459180016 84-85 0.43394859794487295 86-87 0.4353812969109418 88-89 0.44143047032323285 90-91 0.4632393323622818 92-93 0.46737824048648086 94-95 0.46514959765037367 96-97 0.4642740593933316 98-99 0.4598167737211172 100-101 0.440395743292183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 628185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.38342052556355 #Duplication Level Percentage of deduplicated Percentage of total 1 83.58796878722556 40.44271844710015 2 9.817269778308656 9.499861841936276 3 2.824687450759972 4.100041223502054 4 1.145212244361784 2.2163714243992256 5 0.6160699892089434 1.490378668053785 6 0.36057299254815756 1.0467452837171038 7 0.2556307831666222 0.8657804176860882 8 0.18912440627488805 0.7320388544356351 9 0.1418968338937149 0.6178908766973081 >10 0.8874002146787099 8.257376485537051 >50 0.07934618414492932 2.698478588555139 >100 0.07276143795004827 7.602254505756788 >500 0.01020635554955428 3.344106295351443 >1k 0.01020635554955428 10.367956096758157 >5k 0.0013169491031682944 4.509676998113625 >10k+ 3.292372757920736E-4 2.208323992400183 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13863 2.206833974068149 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7821 1.2450154015138852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7227 1.150457269753337 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6672 1.0621075001790874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6590 1.0490540207104595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4970 0.7911682068180551 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4480 0.7131657075543032 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3498 0.5568423314787841 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3093 0.492370878005683 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3076 0.48966466884755283 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3013 0.47963577608507046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2977 0.4739049802207948 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2694 0.4288545571766279 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2665 0.4242380827304058 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2585 0.4115029808097933 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2503 0.3984495013411654 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2345 0.37329767504795563 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2215 0.35260313442696023 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1830 0.29131545643401224 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1772 0.28208250754156816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1689 0.2688698392989326 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1668 0.26552687504477185 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1635 0.26027364550251914 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1533 0.24403639055373816 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1457 0.23193804372915622 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1455 0.2316196661811409 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1398 0.22254590606270447 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1383 0.2201580744525896 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1267 0.2016921766677014 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1237 0.19691651344747166 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1197 0.1905489624871654 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1194 0.19007139616514243 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1158 0.1843406003008668 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1076 0.17128712083223893 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1017 0.16189498316578715 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1006 0.16014390665170292 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.14900069247116693 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 905 0.14406584047692955 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 864 0.13753910074261563 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 859 0.13674315687257735 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 859 0.13674315687257735 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 853 0.13578802422853142 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 833 0.13260424874837826 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 817 0.13005722836425576 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 778 0.12384886617795712 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 753 0.11986914682776571 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 719 0.11445672851150537 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 715 0.11381997341547473 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 712 0.11334240709345177 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 702 0.1117505193533752 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 687 0.10936268774326034 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 677 0.10777080000318377 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 630 0.1002889276248239 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 630 0.1002889276248239 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5918877400765698E-4 0.0 8 0.0 0.0 0.0 1.5918877400765698E-4 0.0 9 0.0 0.0 0.0 1.5918877400765698E-4 0.0 10-11 0.0 0.0 0.0 3.1837754801531396E-4 0.0 12-13 0.0 0.0 0.0 3.1837754801531396E-4 0.0 14-15 0.0 0.0 0.0 6.367550960306279E-4 0.0 16-17 0.0 0.0 0.0 8.755382570421134E-4 0.0 18-19 0.0 0.0 0.0 0.0012735101920612558 0.0 20-21 0.0 0.0 0.0 0.002308237223111026 7.959438700382849E-5 22-23 0.0 0.0 0.0 0.005332823929256509 1.5918877400765698E-4 24-25 0.0 0.0 0.0 0.013053479468627872 1.5918877400765698E-4 26-27 0.0 0.0 0.0 0.027460063516320826 1.5918877400765698E-4 28-29 0.0 0.0 0.0 0.07052062688539204 1.5918877400765698E-4 30-31 0.0 0.0 0.0 0.17661994476149542 1.5918877400765698E-4 32-33 0.0 0.0 0.0 0.3732180806609518 1.5918877400765698E-4 34-35 0.0 0.0 0.0 0.7361684853984096 1.5918877400765698E-4 36-37 0.0 0.0 0.0 1.278126666507478 1.5918877400765698E-4 38-39 0.0 0.0 0.0 1.978238894593153 1.5918877400765698E-4 40-41 0.0 0.0 0.0 2.781585042622794 1.5918877400765698E-4 42-43 0.0 0.0 0.0 3.505734775583626 1.5918877400765698E-4 44-45 0.0 0.0 0.0 4.2837699085460486 1.5918877400765698E-4 46-47 0.0 0.0 0.0 5.150871160565757 1.5918877400765698E-4 48-49 0.0 0.0 0.0 6.029991165023042 1.5918877400765698E-4 50-51 0.0 0.0 0.0 6.936412044222641 1.5918877400765698E-4 52-53 0.0 0.0 0.0 7.754403559460987 1.5918877400765698E-4 54-55 0.0 0.0 0.0 8.596512173961493 1.5918877400765698E-4 56-57 0.0 0.0 0.0 9.522911244299053 1.5918877400765698E-4 58-59 0.0 0.0 0.0 10.563050693665083 1.5918877400765698E-4 60-61 0.0 0.0 0.0 11.812205003303166 1.5918877400765698E-4 62-63 0.0 0.0 0.0 12.926208043808751 1.5918877400765698E-4 64-65 0.0 0.0 0.0 13.930211641475044 1.5918877400765698E-4 66-67 0.0 0.0 0.0 15.034504166766158 1.5918877400765698E-4 68-69 0.0 0.0 0.0 16.237175354394008 1.5918877400765698E-4 70-71 0.0 0.0 0.0 17.58478792075583 1.5918877400765698E-4 72-73 0.0 0.0 0.0 18.877400765698003 1.5918877400765698E-4 74-75 0.0 0.0 0.0 20.153696761304392 1.5918877400765698E-4 76-77 0.0 0.0 0.0 21.389717997086848 1.5918877400765698E-4 78-79 0.0 0.0 0.0 22.71010928309336 1.5918877400765698E-4 80-81 0.0 0.0 0.0 24.088922849160674 1.5918877400765698E-4 82-83 0.0 0.0 0.0 25.45858306072256 1.5918877400765698E-4 84-85 0.0 0.0 0.0 26.9708764137953 1.5918877400765698E-4 86-87 0.0 0.0 0.0 28.50537660084211 1.5918877400765698E-4 88-89 0.0 0.0 0.0 29.874798029242978 1.5918877400765698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 75 2.402885E-9 50.61958 1 CTATACT 70 2.0732035E-4 33.870007 4 TATCACG 60 0.003983136 31.61201 2 ATTAGAC 100 5.0081944E-5 28.450808 3 TCGCAAT 35 0.008464514 27.094925 24-25 CGGGAGT 685 0.0 26.997116 4 TACCTGG 480 0.0 26.672632 2 GTCGGGA 660 0.0 26.582823 2 GTGCAGG 90 8.9916575E-4 26.364367 1 CTAGACT 90 9.0336474E-4 26.343338 4 GTCACTA 200 7.239578E-10 26.100723 1 CTTAACG 55 9.584063E-5 25.86643 20-21 TCGGGAG 680 0.0 25.800974 3 ATGGGCA 315 0.0 25.590672 5 CATGGGT 510 0.0 25.103653 4 ATGGGAG 1375 0.0 24.829794 5 TGGGAGT 370 0.0 24.343964 6 ACCTGGG 565 0.0 23.49919 3 GTCTTAG 125 2.2685477E-4 22.778812 1 GGTCGGG 775 0.0 22.656347 1 >>END_MODULE