##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939316_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 637831 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933079452080566 33.0 31.0 34.0 30.0 34.0 2 31.90813083716533 33.0 31.0 34.0 30.0 34.0 3 31.942809302150568 33.0 31.0 34.0 30.0 34.0 4 35.47805766731313 37.0 35.0 37.0 33.0 37.0 5 35.552511558704424 37.0 35.0 37.0 33.0 37.0 6 35.71990072605439 37.0 35.0 37.0 35.0 37.0 7 35.66451928488895 37.0 35.0 37.0 33.0 37.0 8 35.67777985077552 37.0 35.0 37.0 33.0 37.0 9 37.25238973960187 39.0 37.0 39.0 34.0 39.0 10-11 37.18575218200432 39.0 37.0 39.0 34.0 39.0 12-13 37.096417389559306 39.0 37.0 39.0 33.5 39.0 14-15 38.11574852899906 40.0 38.0 41.0 33.0 41.0 16-17 38.126294425952956 40.0 38.0 41.0 33.0 41.0 18-19 38.209718561813396 40.0 38.0 41.0 33.5 41.0 20-21 38.17350677530568 40.0 38.0 41.0 34.0 41.0 22-23 38.047012453141974 40.0 38.0 41.0 33.5 41.0 24-25 37.976155909637505 40.0 37.5 41.0 33.0 41.0 26-27 37.75020185597752 40.0 37.0 41.0 33.0 41.0 28-29 37.516410303042655 40.0 36.0 41.0 33.0 41.0 30-31 37.13026099389964 39.0 35.5 41.0 33.0 41.0 32-33 36.67046756899555 39.0 35.0 41.0 32.0 41.0 34-35 36.23377979433424 38.5 35.0 41.0 31.0 41.0 36-37 35.82477568509527 38.0 35.0 41.0 30.0 41.0 38-39 35.32275007015965 38.0 35.0 40.0 28.5 41.0 40-41 34.97670150870685 38.0 35.0 40.0 27.5 41.0 42-43 34.58859948795214 38.0 35.0 40.0 24.5 41.0 44-45 34.03584335035456 38.0 35.0 40.0 19.5 41.0 46-47 33.2960870512722 38.0 33.5 40.0 5.0 41.0 48-49 32.738395437035834 38.0 33.0 40.0 2.0 41.0 50-51 31.4508459137295 36.5 31.5 39.0 2.0 40.5 52-53 31.425652719921107 36.5 32.0 39.5 2.0 40.5 54-55 31.808311292489705 37.0 33.0 40.0 2.0 41.0 56-57 31.67657341835063 37.0 33.0 40.0 2.0 41.0 58-59 31.43858796452352 36.5 32.5 40.0 2.0 41.0 60-61 31.06847738664317 36.0 31.5 40.0 2.0 41.0 62-63 30.625786454405635 35.5 31.0 39.5 2.0 41.0 64-65 30.16899460828966 35.0 30.0 39.0 2.0 41.0 66-67 29.739055486484666 35.0 29.5 39.0 2.0 41.0 68-69 29.291709716210093 35.0 29.0 38.0 2.0 40.0 70-71 28.782960533432835 35.0 28.5 37.0 2.0 39.5 72-73 28.244957520095447 35.0 26.5 37.0 2.0 39.0 74-75 27.748460015270503 35.0 26.0 36.0 2.0 39.0 76-77 27.20938696927556 34.5 24.5 35.5 2.0 38.0 78-79 26.698409923631807 34.0 22.5 35.0 2.0 37.0 80-81 26.14648080761205 34.0 19.0 35.0 2.0 37.0 82-83 25.59440274931761 34.0 10.0 35.0 2.0 36.0 84-85 25.062403677463152 33.5 2.0 35.0 2.0 36.0 86-87 24.613211022982576 33.0 2.0 35.0 2.0 35.0 88-89 24.05633937516364 33.0 2.0 35.0 2.0 35.0 90-91 23.45451067759328 33.0 2.0 35.0 2.0 35.0 92-93 22.89029068828577 32.0 2.0 35.0 2.0 35.0 94-95 22.285441598166287 32.0 2.0 35.0 2.0 35.0 96-97 21.599031718433253 31.0 2.0 35.0 2.0 35.0 98-99 20.828692083012584 31.0 2.0 35.0 2.0 35.0 100-101 18.940595549604833 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31144713756415143 1101 2 0.0893980794918896 1101 3 0.41468460026250753 1101 4 0.5014964564021369 1101 5 0.3775267759812735 1101 6 0.4536849901899771 1101 7 0.4532379256426893 1101 8 0.4860932630649799 1101 9 0.46996752796016494 1101 10-11 0.715050628435506 1101 12-13 0.5277596296575737 1101 14-15 0.6026457358478368 1101 16-17 0.7489813166421655 1101 18-19 0.7868161029520664 1101 20-21 0.8418954377395806 1101 22-23 0.7825839147028901 1101 24-25 0.7452231197928825 1101 26-27 0.7085612061875963 1101 28-29 0.6513528675975238 1101 30-31 0.6905095203743699 1101 32-33 0.7323570668413879 1101 34-35 0.6386674238761927 1101 36-37 0.7700503103993483 1101 38-39 0.7312822971179997 1101 40-41 0.5833406404900003 1101 42-43 0.2451311021810909 1101 44-45 0.4960680044838597 1101 46-47 0.47867181807005466 1101 48-49 0.7092328004201249 1101 50-51 0.7378603105729518 1101 52-53 0.6818007678166964 1101 54-55 0.6297653418474738 1101 56-57 0.6140811372523451 1101 58-59 0.41101683612448525 1101 60-61 0.5601853056492914 1101 62-63 0.6491473160011658 1101 64-65 0.6851019429929828 1101 66-67 0.4643623667293184 1101 68-69 0.40576824311401083 1101 70-71 0.5658483859107513 1101 72-73 0.33565397471339153 1101 74-75 0.5319430638137419 1101 76-77 0.2410111387071261 1101 78-79 0.15776796216988842 1101 80-81 0.16370256797961602 1101 82-83 0.18737394988639977 1101 84-85 0.2476437531038087 1101 86-87 -0.15515757308339673 1101 88-89 -0.30722418136455687 1101 90-91 0.10278668885798581 1101 92-93 0.24309475976779993 1101 94-95 0.1854533165890011 1101 96-97 0.047378721021729575 1101 98-99 -0.27681157281149993 1101 100-101 -0.2200315994472284 1102 1 0.598238484537724 1102 2 0.5454136378891654 1102 3 0.7123987946359058 1102 4 0.5691648280986428 1102 5 0.4132524793060881 1102 6 0.5702930464559657 1102 7 0.5248491788396166 1102 8 0.5823741667307942 1102 9 0.6883711937743158 1102 10-11 0.7810272908395888 1102 12-13 0.8597076262475198 1102 14-15 0.9453833743815068 1102 16-17 0.9251721734196607 1102 18-19 0.982907854870227 1102 20-21 0.8904488264863843 1102 22-23 0.7050450187097113 1102 24-25 0.8069904897843614 1102 26-27 0.8672488660341457 1102 28-29 0.6520055743323923 1102 30-31 0.7290991410052285 1102 32-33 0.628310285085206 1102 34-35 0.4224216864507824 1102 36-37 0.7066178722066851 1102 38-39 0.7370900550037618 1102 40-41 0.7175051758693698 1102 42-43 0.6543782249432368 1102 44-45 0.5257728211931507 1102 46-47 0.21928083341532556 1102 48-49 0.387981201954652 1102 50-51 0.3755015083564146 1102 52-53 0.4052529204168671 1102 54-55 0.34417284397876813 1102 56-57 0.3131566913870394 1102 58-59 0.11953665709635786 1102 60-61 0.1602892059050447 1102 62-63 0.1824220389338187 1102 64-65 0.10427287223424386 1102 66-67 -0.04991725816411474 1102 68-69 0.12874085607223407 1102 70-71 0.190981591366846 1102 72-73 0.2635898229657343 1102 74-75 0.261509346848694 1102 76-77 0.361362268289394 1102 78-79 0.03100965014942858 1102 80-81 0.09855177940331572 1102 82-83 0.03530660120780027 1102 84-85 0.1825862083467733 1102 86-87 0.15498469034371354 1102 88-89 -0.06899954368340033 1102 90-91 0.05045026515807294 1102 92-93 0.29214100870215276 1102 94-95 0.5876538707236953 1102 96-97 0.4630863084045096 1102 98-99 0.5877184783393972 1102 100-101 0.6738436136815018 1103 1 0.31282315720460474 1103 2 0.3699575948161957 1103 3 0.31105745208796165 1103 4 0.36839872930939777 1103 5 0.22595672767209862 1103 6 0.08854481437624884 1103 7 0.21891860989072853 1103 8 0.1846657664307827 1103 9 0.42839985551223236 1103 10-11 0.35853284907381777 1103 12-13 0.3190253697383554 1103 14-15 0.3234671898749184 1103 16-17 0.3660221814556621 1103 18-19 0.49640666286337165 1103 20-21 0.4168588098786472 1103 22-23 0.28359860544495774 1103 24-25 0.3295364958917233 1103 26-27 0.5226500633983306 1103 28-29 0.4482711775682233 1103 30-31 0.7359280679311979 1103 32-33 0.828485402946562 1103 34-35 0.775754617984056 1103 36-37 0.6350271457521686 1103 38-39 0.6871061270063308 1103 40-41 0.6119054223067479 1103 42-43 0.8216090858668466 1103 44-45 0.8176705228967833 1103 46-47 1.0313070441728556 1103 48-49 1.2618779259447948 1103 50-51 1.269196930514351 1103 52-53 1.2945452835369764 1103 54-55 1.3417218635866135 1103 56-57 1.4562695034517539 1103 58-59 1.2673019790721405 1103 60-61 1.2448095235949665 1103 62-63 1.294480728529873 1103 64-65 1.4473029804523954 1103 66-67 1.2803222146742002 1103 68-69 1.1680385765265378 1103 70-71 1.3745272090674803 1103 72-73 1.3996091897288316 1103 74-75 1.4585487500416647 1103 76-77 1.468336512905271 1103 78-79 1.2605982068835964 1103 80-81 0.8345071929895163 1103 82-83 0.9262915470987245 1103 84-85 0.8984465962177666 1103 86-87 0.6412113189733084 1103 88-89 0.9006042814532123 1103 90-91 0.803170786426687 1103 92-93 1.0559461892443167 1103 94-95 1.0929877665771208 1103 96-97 0.7454513035829109 1103 98-99 0.6492842931899254 1103 100-101 0.5358437074348466 1104 1 0.07903589609493622 1104 2 0.12795915609644837 1104 3 0.07157440932622805 1104 4 0.020638009963981574 1104 5 0.1095546112699779 1104 6 -0.039595816496621694 1104 7 0.13966918091452385 1104 8 0.17113467093247436 1104 9 0.11565229139854694 1104 10-11 0.0936067207597091 1104 12-13 0.23230203438114216 1104 14-15 0.20907449897949704 1104 16-17 0.05625261485549515 1104 18-19 -0.1731946324209872 1104 20-21 0.044233256925224396 1104 22-23 0.12775970643665602 1104 24-25 -0.07918643468914155 1104 26-27 0.03365130664001015 1104 28-29 0.02517868616971697 1104 30-31 0.059691331585170815 1104 32-33 0.30665639204651285 1104 34-35 0.47322765698714164 1104 36-37 0.5974552256665859 1104 38-39 0.45485123354924895 1104 40-41 0.4282959736699752 1104 42-43 0.45600929403754975 1104 44-45 0.8033009620791418 1104 46-47 0.7741150356147202 1104 48-49 0.4658273867248539 1104 50-51 0.5182500429554402 1104 52-53 0.6888841970015775 1104 54-55 0.351422039953448 1104 56-57 0.5033102991264293 1104 58-59 0.5697422022773928 1104 60-61 0.24220738984528722 1104 62-63 0.10163370508175618 1104 64-65 -0.026964808023041797 1104 66-67 -0.32278010700735393 1104 68-69 -0.1505493322253031 1104 70-71 -0.17810817416899738 1104 72-73 -0.48190698686930844 1104 74-75 -0.20797335363890923 1104 76-77 -0.016498829853567543 1104 78-79 -0.15928498622363918 1104 80-81 -0.09883481412298423 1104 82-83 -0.03631000839900622 1104 84-85 -0.23375625456431948 1104 86-87 -0.48149374362246533 1104 88-89 -0.7428311133483021 1104 90-91 -0.4105366759146527 1104 92-93 -0.4170060760576746 1104 94-95 -0.4480247525774743 1104 96-97 -0.39390741501077997 1104 98-99 -0.8264291251464435 1104 100-101 -1.2374526177740428 1105 1 0.12075608665497128 1105 2 0.1550429947252745 1105 3 0.33034858490855257 1105 4 0.07852765642181936 1105 5 0.10522479030179 1105 6 0.073997605529172 1105 7 0.17577797140928197 1105 8 0.09667432652306474 1105 9 0.2155046702548873 1105 10-11 0.3089644100845703 1105 12-13 0.23490112469952606 1105 14-15 0.46085388771417257 1105 16-17 0.24561341230910472 1105 18-19 0.21074760070064968 1105 20-21 0.2045264630588406 1105 22-23 0.3393969237331831 1105 24-25 0.28984246971045735 1105 26-27 0.291420879916096 1105 28-29 0.49300446327676184 1105 30-31 0.5317166757101219 1105 32-33 0.192937207585949 1105 34-35 0.06133172839625445 1105 36-37 0.24018361174528735 1105 38-39 0.38824355545317246 1105 40-41 0.3596641192429857 1105 42-43 0.034323358769263734 1105 44-45 -0.09007345364954489 1105 46-47 -0.15555531708901782 1105 48-49 -0.10535714569340371 1105 50-51 -0.2293387617923912 1105 52-53 -0.3791383084855582 1105 54-55 -0.6330942088525546 1105 56-57 -0.7562483945013021 1105 58-59 -0.6697528914904822 1105 60-61 -0.5194817793141695 1105 62-63 -0.5252486142170874 1105 64-65 -0.7685424678236821 1105 66-67 -0.48503909977002735 1105 68-69 -0.46689575383488346 1105 70-71 -0.5061340333210573 1105 72-73 -0.4035210578891544 1105 74-75 -0.41918387696035353 1105 76-77 -0.6954123621909822 1105 78-79 -0.5294367695050219 1105 80-81 -0.6105217375246745 1105 82-83 -0.5999113654355632 1105 84-85 -0.7926834411089345 1105 86-87 -0.5573314861268734 1105 88-89 -0.10921601227423139 1105 90-91 -0.22256824827446664 1105 92-93 -0.24272928491614465 1105 94-95 -0.3251563509880775 1105 96-97 -0.3201642159138771 1105 98-99 -0.1462429967429948 1105 100-101 -0.09501468303276894 1106 1 0.06461893206557079 1106 2 0.11854608854384097 1106 3 0.038474864598779135 1106 4 0.22272159964446558 1106 5 0.22392645819996915 1106 6 0.17300826044854034 1106 7 0.295466728184941 1106 8 0.21266291013513694 1106 9 0.09401659146827512 1106 10-11 0.3185462228309959 1106 12-13 0.3246895492492783 1106 14-15 0.3689530929666134 1106 16-17 0.2300047169021724 1106 18-19 0.36142248746745054 1106 20-21 0.2603646707310574 1106 22-23 0.35215575253813114 1106 24-25 0.2790535882795737 1106 26-27 0.33079867549280806 1106 28-29 0.42299870995873334 1106 30-31 0.4187824271103793 1106 32-33 0.2709933757633394 1106 34-35 0.41023803392000247 1106 36-37 0.5072517885639272 1106 38-39 0.3863962891253152 1106 40-41 0.5791306400085148 1106 42-43 0.47861911315899164 1106 44-45 0.5467384748964932 1106 46-47 0.14487505366156483 1106 48-49 0.40130993461050934 1106 50-51 0.14362430980257912 1106 52-53 0.24556768004164553 1106 54-55 0.31126782150465715 1106 56-57 0.394219203601228 1106 58-59 0.38553113628337954 1106 60-61 0.3650386929091525 1106 62-63 0.21703094063403228 1106 64-65 0.34655350541774865 1106 66-67 0.14924729137663206 1106 68-69 0.39230165830010577 1106 70-71 0.2118983883181862 1106 72-73 0.06180533009882083 1106 74-75 0.3479308340482241 1106 76-77 0.1867024334252143 1106 78-79 0.16292288055787196 1106 80-81 0.28353260312815465 1106 82-83 0.49618455704129616 1106 84-85 0.22741227830964306 1106 86-87 0.19516020822528546 1106 88-89 0.31492902774516907 1106 90-91 0.16726981274454644 1106 92-93 0.10313255648405573 1106 94-95 -0.004093002444175653 1106 96-97 0.332211283976136 1106 98-99 0.29997053032298027 1106 100-101 0.15965988786559038 1107 1 0.23032797492324875 1107 2 0.2784077413276371 1107 3 0.20453967272627338 1107 4 0.16272834046010587 1107 5 0.2903689659973807 1107 6 0.15195391128499125 1107 7 0.1468772171080701 1107 8 0.05312224857222958 1107 9 0.11710923488448088 1107 10-11 0.21384289896690234 1107 12-13 0.25914046701667104 1107 14-15 0.30665137191868297 1107 16-17 0.4182647304907974 1107 18-19 0.4808579709069818 1107 20-21 0.5350315085930433 1107 22-23 0.6178294863615079 1107 24-25 0.49542440803764975 1107 26-27 0.633681979134451 1107 28-29 0.5415501300773968 1107 30-31 0.6293178939653572 1107 32-33 0.45407606845169113 1107 34-35 0.641888035792455 1107 36-37 0.5026388772270494 1107 38-39 0.6609320555721041 1107 40-41 0.23601777414539526 1107 42-43 0.3229290087833476 1107 44-45 0.47855592718916995 1107 46-47 0.34400998232126767 1107 48-49 0.16454681008195848 1107 50-51 -0.3035044237265616 1107 52-53 -0.20245849144256312 1107 54-55 -0.11399472256476173 1107 56-57 -0.395278766129298 1107 58-59 -0.4437536952764809 1107 60-61 -0.5046295091655253 1107 62-63 -0.4243228933386085 1107 64-65 -0.49736585088785645 1107 66-67 -0.7137777298890384 1107 68-69 -0.548639969446377 1107 70-71 -0.5512007284209695 1107 72-73 -0.6531745277020704 1107 74-75 -0.5344820569592059 1107 76-77 -0.6268431286036638 1107 78-79 -0.278524308361515 1107 80-81 -0.1772797186548445 1107 82-83 -0.2801179985838047 1107 84-85 -0.604510031115197 1107 86-87 -0.44299976309949685 1107 88-89 -0.4042451153420181 1107 90-91 -0.13916178135135127 1107 92-93 0.20625901097552912 1107 94-95 0.12516748728143057 1107 96-97 0.11330062118276274 1107 98-99 0.4529589585894662 1107 100-101 0.6150126839666186 1108 1 0.35476898135183177 1108 2 0.39902142715871847 1108 3 0.5333454425258317 1108 4 0.48109113708377294 1108 5 0.45759810217061414 1108 6 0.41719430696016957 1108 7 0.39018762190915623 1108 8 0.32498082477863477 1108 9 0.2631414865067896 1108 10-11 0.4732411956322835 1108 12-13 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sequence quality scores pass #Quality Count 2 2506.0 3 540.0 4 478.0 5 512.0 6 567.0 7 776.0 8 1046.0 9 1331.0 10 1813.0 11 2984.0 12 4630.0 13 7276.0 14 7626.0 15 9744.0 16 13028.0 17 17884.0 18 13523.0 19 7842.0 20 7428.0 21 6878.0 22 7145.0 23 7658.0 24 8028.0 25 8892.0 26 10114.0 27 11236.0 28 12734.0 29 14454.0 30 16920.0 31 19771.0 32 23200.0 33 28694.0 34 37734.0 35 61090.0 36 71628.0 37 94531.0 38 82090.0 39 13493.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.31356967350346 18.96868028994666 13.896500172138143 17.821249864411737 2 14.362122027737714 24.262103665665908 39.70476299587403 21.671011310722353 3 22.5968722140606 31.741502492434805 29.276377745131278 16.385247548373325 4 12.32946804703637 16.890432631638426 38.34214375851482 32.43795556281038 5 11.654292306967836 41.00585492959957 31.00130283956237 16.338549923870218 6 25.363006511137637 36.054856060095084 19.97245589570886 18.609681533058414 7 23.770387649676763 38.6986102592018 21.49713627742734 16.0338658136941 8 25.033533146436703 29.576784237930813 22.213140600757104 23.17654201487538 9 29.222377530226236 18.65782509181213 22.941972850905884 29.17782452705575 10-11 23.149631126261017 28.156290148255398 26.194663056624435 22.49941566885915 12-13 29.325068103434077 26.386560817758898 27.07257594523174 17.215795133575288 14-15 25.01747134190102 26.203718591782394 31.270603829969133 17.50820623634745 16-17 18.140975161339913 30.3064124968234 31.766785782635843 19.785826559200846 18-19 17.359488956625004 29.025117618728636 37.392323367966604 16.223070056679756 20-21 19.474461188976846 27.229998337051438 35.46551578075435 17.830024693217368 22-23 18.07590237917615 27.932354803105504 34.41227391001821 19.57946890770014 24-25 18.342878212570998 28.08838296670537 35.820362757711735 17.748376063011893 26-27 19.41745287829554 26.75734577673734 36.80427527579514 17.020926069171978 28-29 17.486214911664824 27.77335424205744 37.68818440051241 17.052246445765316 30-31 17.378203996783746 30.12649291051362 36.112882665568435 16.3824204271342 32-33 16.14814408082583 30.054751968874527 35.59089768127765 18.206206269021997 34-35 17.23466575317496 28.66782110228698 35.87578922226447 18.22172392227359 36-37 18.323210758536277 28.635021722696774 35.64850686449804 17.393260654268904 38-39 19.15924634777186 27.49600706499814 36.09039171262507 17.254354874604932 40-41 17.161224680750017 29.008286621654666 36.499191309386866 17.33129738820845 42-43 16.38136463659972 30.442825032756122 34.99478576001284 18.181024570631322 44-45 16.870426609845133 30.291760956275578 33.633644262904994 19.204168170974295 46-47 16.99150935826984 30.353532890420855 32.45141518561578 20.203542565693528 48-49 17.763390275849318 30.120244028115938 32.48555458968742 19.630811106347327 50-51 18.26856288343679 31.00887091508795 32.126368651537526 18.596197549937738 52-53 16.98213690112836 31.300305846963244 32.20195653952411 19.515600712384284 54-55 15.889810178652612 30.58661041398263 32.92653959474786 20.5970398126169 56-57 17.495207104965584 31.1897546069197 30.74844653582601 20.566591752288716 58-59 17.30373545573409 29.671914225489093 31.656908246212534 21.36744207256428 60-61 17.43005704207973 29.22459552622851 32.2693285255982 21.07601890609355 62-63 19.025725680301935 28.72765194084926 31.95871748309715 20.28790489575166 64-65 18.026205861390252 29.406623525172954 32.721532828914555 19.84563778452224 66-67 17.29172648994379 29.419284795512567 32.30519901975053 20.983789694793114 68-69 17.17438991382946 30.30501161319561 31.989711778738727 20.530886694236205 70-71 17.12295085191844 30.26244044832429 31.06624546109028 21.548363238666987 72-73 17.318146131322777 29.19637055346385 30.97789574545414 22.507587569759227 74-75 17.365548340605102 29.616497499067755 31.48539169209032 21.532562468236822 76-77 17.787007102909598 28.53379584837687 32.19678913876238 21.482407909951153 78-79 17.447167923899162 28.249353558104275 32.982377773723314 21.321100744273245 80-81 17.206698374950456 29.69325066530774 32.04589207859125 21.05415888115056 82-83 16.951112180780402 29.0264200449733 31.371355357509433 22.65111241673686 84-85 17.561993546976346 27.935756075076572 31.321941696254825 23.180308681692257 86-87 18.261972759313107 27.600445805316472 31.452477452145537 22.685103983224884 88-89 18.910318938716944 26.459940718178498 31.92486564045764 22.704874702646915 90-91 18.461838661910104 26.644798441475874 32.14975301861763 22.74360987799639 92-93 18.559614541855396 27.181832987544524 31.856394181936054 22.40215828866403 94-95 18.8434086424532 26.94582652781843 31.469172984387438 22.741591845340935 96-97 19.1262325367676 26.033552695766023 30.84772407542039 23.992490692045987 98-99 19.89031340376185 25.534665118372153 30.780614219763414 23.794407258102584 100-101 20.896708116403257 24.79936507886567 31.919810530472205 22.384116274258872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4071.0 1 2898.5 2 2602.0 3 3316.0 4 2486.0 5 2194.0 6 3839.0 7 6554.0 8 8674.0 9 7975.0 10 5691.0 11 4506.5 12 4188.5 13 4067.5 14 3910.5 15 3735.5 16 3640.0 17 3723.5 18 3912.5 19 4123.5 20 4369.5 21 4689.0 22 5025.5 23 5434.5 24 5762.5 25 6162.5 26 6794.5 27 7411.0 28 7953.0 29 8534.0 30 9154.5 31 9665.5 32 10199.0 33 10917.5 34 11684.5 35 12158.0 36 12429.5 37 13120.5 38 13377.5 39 12898.5 40 12803.0 41 13072.0 42 13252.0 43 13561.0 44 14226.0 45 14957.5 46 18447.0 47 25158.0 48 42987.0 49 46155.5 50 31109.5 51 22325.0 52 14885.0 53 11309.5 54 9759.5 55 8866.5 56 8393.5 57 7905.5 58 7471.5 59 7087.0 60 6870.5 61 6752.5 62 5797.5 63 3564.5 64 1804.5 65 1339.0 66 1057.5 67 819.5 68 649.0 69 517.5 70 458.5 71 421.0 72 392.0 73 427.0 74 400.0 75 313.5 76 226.5 77 139.0 78 101.0 79 79.0 80 54.5 81 27.5 82 19.5 83 19.5 84 12.0 85 7.5 86 5.0 87 3.0 88 1.5 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26872321978705954 2 0.10159430946441925 3 0.1102172832615536 4 0.11084440862861793 5 0.1193106010839862 6 0.04828865326395237 7 0.05863622182051358 8 0.0630260993899638 9 0.060831160605238695 10-11 0.05487346961812768 12-13 0.05981208188375917 14-15 0.056206111023139355 16-17 0.05597093901049024 18-19 0.061144723288770846 20-21 0.06412356878232635 22-23 0.07000286909855433 24-25 0.07697963880714484 26-27 0.07713642014891092 28-29 0.07172746385798119 30-31 0.06953252507325608 32-33 0.06443713146585851 34-35 0.22654903885198432 36-37 0.34578124926508746 38-39 0.3167767010383628 40-41 0.3020392549123514 42-43 0.32485094013931587 44-45 0.33809896351854957 46-47 0.34335113846771326 48-49 0.35252284696102887 50-51 0.3471922813409822 52-53 0.3478977973789295 54-55 0.3336306952782163 56-57 0.3126219954815617 58-59 0.2954544385581761 60-61 0.25618071244577323 62-63 0.3238318614178364 64-65 0.3524444562901458 66-67 0.35722628721401123 68-69 0.36796580912498766 70-71 0.36898488784646716 72-73 0.35307158165721014 74-75 0.3555800831254674 76-77 0.3411561996829881 78-79 0.3203042812280996 80-81 0.31905003049397096 82-83 0.33245483521497077 84-85 0.33864769821473084 86-87 0.3335523046073333 88-89 0.34852492274599384 90-91 0.36969040388441454 92-93 0.3649085729605491 94-95 0.3632623688720053 96-97 0.3689064971755841 98-99 0.3594996166696194 100-101 0.33888287022738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 637831.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07168743062522 #Duplication Level Percentage of deduplicated Percentage of total 1 83.41420986881046 40.0986182408603 2 9.910128152554295 9.527931658940588 3 2.8282352269698463 4.078741194335334 4 1.1649441668352418 2.2400332744893543 5 0.6199226234330599 1.4900363292423617 6 0.39150839242698066 1.1292281440329819 7 0.2779781244783305 0.935401425873161 8 0.18081250404710583 0.6953569742480903 9 0.1428923695076444 0.6182169590873594 >10 0.8967805664498457 8.372593272066466 >50 0.07648786601244052 2.5733591479837896 >100 0.0738728955622454 7.686451032932314 >500 0.011113621456267002 3.7163410139600157 >1k 0.00915239414045518 9.345298949907319 >5k 0.0016343560965098533 5.290494507590368 >10k+ 3.268712193019707E-4 2.201897874450201 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14013 2.1969769421680665 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7987 1.2522125766856738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7427 1.1644150252966696 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6620 1.0378924824914437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6608 1.0360111063902506 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5027 0.7881398050580796 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4607 0.7222916415163264 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3672 0.5757010869650424 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3157 0.49495869595551173 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3144 0.4929205385125527 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3144 0.4929205385125527 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3125 0.4899416930189972 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2741 0.4297376577808228 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2716 0.4258181242366708 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2618 0.41045355274359513 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2452 0.38442785001042595 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2328 0.3649869636314322 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2313 0.362635243504941 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1835 0.28769376214075515 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1821 0.28549882335603005 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1708 0.2677825317364631 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1634 0.25618071244577323 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1623 0.2544561176863464 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1550 0.24301107973742261 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1545 0.2422271730285922 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1455 0.2281168522696451 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1450 0.22733294556081468 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1443 0.22623547616845213 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1304 0.20444286966296715 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1273 0.19958264806821868 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1242 0.19472242647347024 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1238 0.1940953011064059 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1208 0.18939186085342358 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1128 0.17684935351213724 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1000 0.1567813417660791 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 990 0.1552135283484183 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 932 0.14612021052598573 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.14361170905772846 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 865 0.1356158606276584 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 854 0.13389126586823155 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 846 0.13263701513410292 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 825 0.12934460695701525 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 823 0.1290310442734831 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 803 0.12589541743816152 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 760 0.11915381974222013 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 745 0.11680209961572893 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 743 0.11648853693219678 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 724 0.11350969143864127 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 708 0.111001189970384 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 707 0.11084440862861793 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 702 0.11006050191978753 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 697 0.10927659521095714 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 640 0.10034005873029062 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.703440252982373E-4 0.0 18-19 0.0 0.0 0.0 9.406880505964746E-4 0.0 20-21 0.0 0.0 0.0 0.001959766772075989 0.0 22-23 0.0 0.0 0.0 0.005095393607397572 0.0 24-25 0.0 0.0 0.0 0.011915381974222011 0.0 26-27 0.0 0.0 0.0 0.023830763948444023 0.0 28-29 0.0 0.0 0.0 0.06130150463053693 0.0 30-31 0.0 0.0 0.0 0.1575652484749095 0.0 32-33 0.0 0.0 0.0 0.348916876100409 0.0 34-35 0.0 0.0 0.0 0.7046537405676425 0.0 36-37 0.0 0.0 0.0 1.237083177205247 0.0 38-39 0.0 0.0 0.0 1.926293955608931 0.0 40-41 0.0 0.0 0.0 2.725878798615934 0.0 42-43 0.0 0.0 0.0 3.4237909414876353 0.0 44-45 0.0 0.0 0.0 4.166777720117084 0.0 46-47 0.0 0.0 0.0 5.02186315810928 0.0 48-49 0.0 0.0 0.0 5.914576118125334 0.0 50-51 0.0 0.0 0.0 6.816147223951172 0.0 52-53 0.0 0.0 0.0 7.632664451868912 0.0 54-55 0.0 0.0 0.0 8.446986741001925 0.0 56-57 0.0 0.0 0.0 9.36211943289053 0.0 58-59 0.0 0.0 0.0 10.386136766635676 0.0 60-61 0.0 0.0 0.0 11.629647978853333 0.0 62-63 0.0 0.0 0.0 12.724844041760278 0.0 64-65 0.0 0.0 0.0 13.72040556197488 0.0 66-67 0.0 0.0 0.0 14.808860027185885 0.0 68-69 0.0 0.0 0.0 15.994754096304508 0.0 70-71 0.0 0.0 0.0 17.301604970595662 0.0 72-73 0.0 0.0 0.0 18.614256754532157 0.0 74-75 0.0 0.0 0.0 19.915777063203265 0.0 76-77 0.0 0.0 0.0 21.147294502775814 0.0 78-79 0.0 0.0 0.0 22.476329936926867 0.0 80-81 0.0 0.0 0.0 23.8780335229865 0.0 82-83 0.0 0.0 0.0 25.246656245933487 0.0 84-85 0.0 0.0 0.0 26.79753727868354 0.0 86-87 0.0 0.0 0.0 28.362842194876073 0.0 88-89 0.0 0.0 0.0 29.70700702850755 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 105 0.0 58.822716 1 TGTTAGG 65 0.005882979 29.189129 2 AACCGAG 65 0.005903318 29.168522 7 TAGCACA 100 4.998021E-5 28.4594 4 TACCTGG 485 0.0 28.361603 2 ATGGGCG 105 6.968375E-5 27.104193 5 ATGGGAC 690 0.0 26.122156 5 TGGACCA 110 9.5623465E-5 25.872183 5 GTCACTA 260 0.0 25.540487 2 CATGGGG 1670 0.0 25.278309 4 TCACTAC 285 0.0 24.964386 3 TAAGGTG 95 0.0012361843 24.964386 5 TGGGACT 535 0.0 23.920914 6 CACTACC 300 0.0 23.716166 4 ATGGGTG 225 2.8539944E-9 23.18914 5 TACCTCC 455 0.0 22.934315 2 CGGGAGT 705 0.0 22.875168 4 ATGGGGA 770 0.0 22.79216 5 CTTAGAG 125 2.2762336E-4 22.76752 2 CTACCTC 460 0.0 22.722486 1 >>END_MODULE