##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939316_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 637831 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.002773461935845 33.0 31.0 34.0 30.0 34.0 2 32.13556255497146 33.0 31.0 34.0 30.0 34.0 3 32.215542988660005 34.0 31.0 34.0 30.0 34.0 4 35.82011065627102 37.0 35.0 37.0 35.0 37.0 5 35.76597876239944 37.0 35.0 37.0 33.0 37.0 6 35.86704942218236 37.0 35.0 37.0 35.0 37.0 7 35.799243059681956 37.0 35.0 37.0 33.0 37.0 8 35.83181751906069 37.0 35.0 37.0 33.0 37.0 9 37.40525938689088 39.0 37.0 39.0 34.0 39.0 10-11 37.33924346731344 39.0 37.0 39.0 34.0 39.0 12-13 37.26563384344756 39.0 37.0 39.0 33.5 39.0 14-15 38.27109218586114 40.0 37.5 41.0 33.0 41.0 16-17 38.28834048517554 40.0 37.5 41.0 33.5 41.0 18-19 38.50354796176417 40.0 38.0 41.0 34.0 41.0 20-21 38.51526344752764 40.0 38.0 41.0 34.0 41.0 22-23 38.42258607687616 40.0 38.0 41.0 34.0 41.0 24-25 38.30034052907432 40.0 37.5 41.0 34.0 41.0 26-27 37.92620537415083 40.0 36.5 41.0 33.0 41.0 28-29 37.64906534803106 40.0 36.0 41.0 33.0 41.0 30-31 37.2261421912701 39.0 35.5 41.0 33.0 41.0 32-33 36.739706912959704 38.5 35.0 41.0 32.0 41.0 34-35 36.409743333265396 38.5 35.0 41.0 31.0 41.0 36-37 36.08531648665556 38.0 35.0 41.0 30.5 41.0 38-39 35.74879552734188 38.0 35.0 41.0 30.0 41.0 40-41 35.32851100056284 38.0 35.0 40.5 28.5 41.0 42-43 34.91323093421298 38.0 35.0 40.0 26.0 41.0 44-45 34.30700530391279 38.0 34.5 40.0 23.0 41.0 46-47 33.47466727079744 38.0 33.5 40.0 11.0 41.0 48-49 32.76616298047602 38.0 33.0 40.0 2.0 41.0 50-51 32.36249414029735 37.0 33.0 40.0 2.0 41.0 52-53 31.975200327359445 37.0 33.0 40.0 2.0 41.0 54-55 31.644671550928066 36.5 32.0 40.0 2.0 41.0 56-57 31.37768938794132 36.0 31.5 40.0 2.0 41.0 58-59 31.043905046948172 36.0 31.0 40.0 2.0 41.0 60-61 30.61198264116984 35.0 30.5 39.5 2.0 41.0 62-63 30.138535913118055 35.0 29.5 39.0 2.0 40.5 64-65 29.62457061510024 35.0 29.0 39.0 2.0 40.0 66-67 29.192285260515717 35.0 28.0 38.0 2.0 40.0 68-69 28.80179310820578 35.0 28.0 37.0 2.0 40.0 70-71 28.39061052222297 35.0 26.5 37.0 2.0 39.0 72-73 27.873671082151855 34.0 26.0 36.0 2.0 39.0 74-75 27.386718425413626 34.0 25.5 35.5 2.0 38.5 76-77 25.507026939737955 31.5 21.5 34.5 2.0 36.0 78-79 26.312082667665887 33.0 23.0 35.0 2.0 36.5 80-81 26.30788406333339 34.0 23.0 35.0 2.0 36.0 82-83 25.983893852760367 33.0 20.5 35.0 2.0 36.0 84-85 25.628225188176806 33.0 19.0 35.0 2.0 35.5 86-87 25.086726734824744 33.0 11.0 35.0 2.0 35.0 88-89 24.559594939725415 33.0 2.0 35.0 2.0 35.0 90-91 24.086994047012453 32.0 2.0 35.0 2.0 35.0 92-93 23.72918766883391 32.0 2.0 35.0 2.0 35.0 94-95 23.35313664591404 32.0 2.0 34.5 2.0 35.0 96-97 22.82951361724344 31.0 2.0 34.0 2.0 35.0 98-99 22.17422561775768 31.0 2.0 34.0 2.0 35.0 100-101 19.574377852440534 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19638133203670805 1101 2 0.17056898905303086 1101 3 0.29562997025063 1101 4 0.1719283848841826 1101 5 0.2723950426912012 1101 6 0.0882447907577486 1101 7 0.15482287503323988 1101 8 0.29995353075354103 1101 9 0.33840663479037403 1101 10-11 0.3842480868473004 1101 12-13 0.48455631422496737 1101 14-15 0.49412022348952434 1101 16-17 0.572675027748403 1101 18-19 0.39651952952342384 1101 20-21 0.47057011720945496 1101 22-23 0.4267821396171172 1101 24-25 0.46572605094313957 1101 26-27 0.4500595859288339 1101 28-29 0.46552540803941866 1101 30-31 0.2749071038632209 1101 32-33 0.16110838991792065 1101 34-35 0.34794904773521296 1101 36-37 0.4048432966073179 1101 38-39 0.42273223082637656 1101 40-41 0.5078938960583272 1101 42-43 0.33645736295039086 1101 44-45 0.12719243654602508 1101 46-47 0.20293353480658283 1101 48-49 0.29618131586262564 1101 50-51 0.5866526988070788 1101 52-53 0.5924667946993836 1101 54-55 0.5627518292743048 1101 56-57 0.32085449617254014 1101 58-59 0.394538405018384 1101 60-61 0.43537842788888526 1101 62-63 0.4855782806076405 1101 64-65 0.3280428934528281 1101 66-67 0.3379197366850839 1101 68-69 0.36709261638081614 1101 70-71 0.32812311547072426 1101 72-73 0.20912160716785166 1101 74-75 0.06736175174176395 1101 76-77 0.14806218931980197 1101 78-79 0.32283872561144733 1101 80-81 0.4270573556573538 1101 82-83 0.49518374404818033 1101 84-85 0.5398763527623736 1101 86-87 0.4661933229328348 1101 88-89 0.48735855992948274 1101 90-91 0.6286146649594322 1101 92-93 0.5776602549451901 1101 94-95 1.0569195653457726 1101 96-97 1.0033754135135275 1101 98-99 0.46278985333966816 1101 100-101 0.17805662681199408 1102 1 0.3835882800332939 1102 2 0.41998288504620973 1102 3 0.24842195661039312 1102 4 0.236186696904646 1102 5 0.32090846980117504 1102 6 0.4261603917807619 1102 7 0.42842688185335476 1102 8 0.39410314712183236 1102 9 0.6053237277144987 1102 10-11 0.5588537986972568 1102 12-13 0.4068309305932516 1102 14-15 0.6666745713156175 1102 16-17 0.6409295034517228 1102 18-19 0.7070480887732131 1102 20-21 0.5820764045069851 1102 22-23 0.658386466130338 1102 24-25 0.6640156928868706 1102 26-27 0.8219292577105861 1102 28-29 0.5512964012193038 1102 30-31 0.49320420531250164 1102 32-33 0.5075624606766596 1102 34-35 0.7900179948792854 1102 36-37 0.7164241789602599 1102 38-39 0.49963174063029214 1102 40-41 0.5574862873626714 1102 42-43 0.29179357352158064 1102 44-45 0.257027794602287 1102 46-47 0.3275472389839038 1102 48-49 -0.05080557672049224 1102 50-51 -0.18548604799599389 1102 52-53 0.009173423002877712 1102 54-55 0.23647838511402952 1102 56-57 0.5596918789517389 1102 58-59 0.2857095473883753 1102 60-61 0.2505158959195768 1102 62-63 0.2633249771123296 1102 64-65 0.3377935328390187 1102 66-67 0.1420411134966777 1102 68-69 0.20975033164083712 1102 70-71 0.07146284266594805 1102 72-73 0.17606001296495322 1102 74-75 0.09867835446981132 1102 76-77 0.14634383893616842 1102 78-79 0.1946018330283259 1102 80-81 0.620298510815072 1102 82-83 0.30885595206181904 1102 84-85 0.2171861353710689 1102 86-87 0.217392172037691 1102 88-89 0.4910749921545481 1102 90-91 0.4880163060506497 1102 92-93 0.427497744288754 1102 94-95 0.5614219097618012 1102 96-97 0.9352807843575199 1102 98-99 1.0405285575169927 1102 100-101 1.2050973343993796 1103 1 0.31057116335055923 1103 2 0.2926189613346466 1103 3 0.3914761728323981 1103 4 0.2429369379846804 1103 5 0.23623245458635012 1103 6 0.21927353867886978 1103 7 0.36965022911576284 1103 8 0.32446449931298105 1103 9 0.3699659917239302 1103 10-11 0.3928902821430924 1103 12-13 0.3248687399793013 1103 14-15 0.4474691979094203 1103 16-17 0.40905738301885464 1103 18-19 0.3715383384249904 1103 20-21 0.4469995937280231 1103 22-23 0.5299192664089176 1103 24-25 0.5593300344705057 1103 26-27 0.6098709029479181 1103 28-29 0.6284302943940574 1103 30-31 0.7832770650574972 1103 32-33 0.8164847659375667 1103 34-35 0.9388999862004042 1103 36-37 1.1255855552594127 1103 38-39 1.0296311379301955 1103 40-41 1.0181249485648536 1103 42-43 1.0638272845469672 1103 44-45 1.363734172508643 1103 46-47 1.0675440915500687 1103 48-49 1.099054128090387 1103 50-51 1.057479102545102 1103 52-53 1.0829188023021459 1103 54-55 0.739897033994346 1103 56-57 0.8085099170959573 1103 58-59 0.7814672083762666 1103 60-61 0.8345825143702363 1103 62-63 0.513648091330694 1103 64-65 0.6147944971591741 1103 66-67 0.7107171153181717 1103 68-69 0.3197832792461064 1103 70-71 0.374942431222685 1103 72-73 0.477574129540546 1103 74-75 0.5774047152326993 1103 76-77 0.6117907075743325 1103 78-79 0.3519182773656233 1103 80-81 0.589709806754211 1103 82-83 0.6956420872809375 1103 84-85 0.7895051244421083 1103 86-87 0.7817571940027612 1103 88-89 0.9758752305559177 1103 90-91 1.0553094459842214 1103 92-93 0.9393257336812333 1103 94-95 0.9199313654566446 1103 96-97 0.8951945312770491 1103 98-99 0.715947768917399 1103 100-101 0.3471437021267967 1104 1 0.09858400054372396 1104 2 0.12113834723108141 1104 3 0.10321757856287661 1104 4 0.06096105109076433 1104 5 0.10531621438049399 1104 6 0.13820261607789064 1104 7 0.12184035835507245 1104 8 -3.6853043808093844E-4 1104 9 0.040998460570605744 1104 10-11 0.11754116253167979 1104 12-13 0.055706916172660215 1104 14-15 0.12473233145671259 1104 16-17 0.13110612965776625 1104 18-19 0.10564363810406263 1104 20-21 0.026480323481976598 1104 22-23 0.05004243407306319 1104 24-25 0.04695544286093423 1104 26-27 -0.0026424691282969093 1104 28-29 -0.020671969462306095 1104 30-31 0.18676681554178032 1104 32-33 0.36744321853535666 1104 34-35 0.23479150613564315 1104 36-37 0.20389532482601425 1104 38-39 -0.007492636898497551 1104 40-41 0.18411216919688655 1104 42-43 0.507303156984598 1104 44-45 0.10107768307620546 1104 46-47 -0.16100998946303235 1104 48-49 -0.23977259871448098 1104 50-51 -0.25270969671473864 1104 52-53 -0.255488691843059 1104 54-55 -0.21901816535801188 1104 56-57 -0.01708929648139801 1104 58-59 0.035421006045911696 1104 60-61 0.2399371273751214 1104 62-63 0.19007275955864245 1104 64-65 0.003293065985612742 1104 66-67 -0.00833917750861346 1104 68-69 0.04498349898184628 1104 70-71 0.11733119000488657 1104 72-73 0.21193964259458298 1104 74-75 -0.017414147345728992 1104 76-77 -0.22307499341158632 1104 78-79 0.10974636306256258 1104 80-81 0.003810480417723028 1104 82-83 0.07673903121941095 1104 84-85 -0.2418361309428647 1104 86-87 -0.40633370720289363 1104 88-89 -0.18720627405564016 1104 90-91 -0.07910521848508623 1104 92-93 -0.025111771739883437 1104 94-95 0.1442786635744362 1104 96-97 -0.06548433650564434 1104 98-99 0.07757207862914228 1104 100-101 0.2889137313285204 1105 1 0.14457711954193542 1105 2 0.2327075929259621 1105 3 0.2328546940517171 1105 4 0.13491834576112893 1105 5 -0.037978102654626866 1105 6 0.1014949568461816 1105 7 0.020251260161558093 1105 8 0.09345024589342898 1105 9 0.02823956212976242 1105 10-11 0.060507898367688995 1105 12-13 0.22690701917809264 1105 14-15 0.26923187182495667 1105 16-17 0.3214385916370901 1105 18-19 0.3178228316075362 1105 20-21 0.2851077762237537 1105 22-23 0.36847741040278237 1105 24-25 0.21791749554341777 1105 26-27 0.3579558606267952 1105 28-29 0.3494603505851259 1105 30-31 0.26272882330910363 1105 32-33 0.11942173112507959 1105 34-35 -0.028104383528777532 1105 36-37 -0.23490627894446447 1105 38-39 -0.0882680521391066 1105 40-41 -0.04180806747604038 1105 42-43 -0.13584892872550114 1105 44-45 0.29732696817664106 1105 46-47 0.12436294107119039 1105 48-49 0.2250124223806722 1105 50-51 0.3399652403291924 1105 52-53 0.14818177464574944 1105 54-55 0.19045384202005167 1105 56-57 0.19111790012421537 1105 58-59 0.06251532487764067 1105 60-61 -0.15886753062591552 1105 62-63 -0.18769962840050525 1105 64-65 -0.15089617054657722 1105 66-67 -0.1553644467749713 1105 68-69 0.09629157802054067 1105 70-71 -0.08729327438188861 1105 72-73 0.04495856984101465 1105 74-75 0.0899974521428355 1105 76-77 0.23509252814020343 1105 78-79 0.19596100360657331 1105 80-81 0.011404136002326482 1105 82-83 -0.007595161490559832 1105 84-85 0.10738668715375255 1105 86-87 0.08331334950698377 1105 88-89 0.4026165506285295 1105 90-91 0.38783872440678735 1105 92-93 0.1082143751686111 1105 94-95 0.04256829446168808 1105 96-97 0.16267267489981307 1105 98-99 0.3771097519642126 1105 100-101 -0.1381096215578772 1106 1 0.13242182507921996 1106 2 0.08293236716463781 1106 3 0.12786792104267874 1106 4 0.09596781865566584 1106 5 -0.11040504397642792 1106 6 0.020282924514788192 1106 7 0.09003280329169883 1106 8 0.006583604421024347 1106 9 0.1311506282421533 1106 10-11 0.17675735546880844 1106 12-13 0.17499037460341071 1106 14-15 0.20124683045182934 1106 16-17 0.23618795566982698 1106 18-19 0.09349278292994967 1106 20-21 0.16492829526328734 1106 22-23 0.33923278721690764 1106 24-25 0.2484340532542717 1106 26-27 0.1766262237062861 1106 28-29 0.13870418409989327 1106 30-31 0.316293906309177 1106 32-33 0.2994907145363399 1106 34-35 0.5887556585494096 1106 36-37 0.5848682135508412 1106 38-39 0.7890805143258177 1106 40-41 0.7485680979939033 1106 42-43 0.7213940474320779 1106 44-45 0.8826904143013294 1106 46-47 0.9102647736722034 1106 48-49 0.8715640978935397 1106 50-51 0.8254761857347148 1106 52-53 0.8386419836829688 1106 54-55 0.7387563311761767 1106 56-57 0.5491815701770655 1106 58-59 0.7022927504370351 1106 60-61 0.5928246217354847 1106 62-63 0.8056135495180357 1106 64-65 0.8624661926005963 1106 66-67 0.8358222596760818 1106 68-69 0.8907035268724144 1106 70-71 1.0544851213448645 1106 72-73 0.936961175755652 1106 74-75 0.8626666532777065 1106 76-77 0.9117123409881529 1106 78-79 0.9611367900343808 1106 80-81 0.8884029909792268 1106 82-83 0.9563789147352608 1106 84-85 0.9250868828793735 1106 86-87 0.9677280622077156 1106 88-89 1.2402474809174926 1106 90-91 1.050391110623643 1106 92-93 1.5061113118087839 1106 94-95 1.3857269224645528 1106 96-97 1.465205544959435 1106 98-99 2.05737192277007 1106 100-101 2.4383333613683043 1107 1 0.004956211263760224 1107 2 0.14943372335254423 1107 3 0.09393914899459332 1107 4 0.1387158244977087 1107 5 0.1365254774738105 1107 6 0.10837361523439881 1107 7 0.08940419548412137 1107 8 0.12845594621248324 1107 9 0.12665140129600871 1107 10-11 0.155383352547787 1107 12-13 0.20899270713962181 1107 14-15 0.30984083540579377 1107 16-17 0.24739322420573018 1107 18-19 0.24827073725290205 1107 20-21 0.2525749481273323 1107 22-23 0.1654093537878083 1107 24-25 0.10753361938114381 1107 26-27 0.23518438464058988 1107 28-29 0.2175382904870844 1107 30-31 0.15463737761523788 1107 32-33 -0.1197893556537366 1107 34-35 0.07671313791234269 1107 36-37 0.2634383046588482 1107 38-39 0.12029664306469812 1107 40-41 0.09052272053497035 1107 42-43 0.12148151753976322 1107 44-45 0.05180998888096866 1107 46-47 0.017043840764721097 1107 48-49 0.32214749943750576 1107 50-51 -0.05364520779517079 1107 52-53 0.05311494284752882 1107 54-55 -0.2525662061524301 1107 56-57 -6.02830719568459E-4 1107 58-59 -0.010856119941362152 1107 60-61 -0.1608373531095033 1107 62-63 -6.33474625853836E-4 1107 64-65 -0.08630895839901953 1107 66-67 -0.24148847587530398 1107 68-69 -0.46885746413448004 1107 70-71 -0.743032279054237 1107 72-73 -0.9249363638466406 1107 74-75 -0.9122074914144349 1107 76-77 -0.7368009346737594 1107 78-79 -0.5156274579634328 1107 80-81 -0.5243315332315355 1107 82-83 -0.4342647261611603 1107 84-85 -0.7444432929703133 1107 86-87 -0.637872699611453 1107 88-89 -0.35966237404797496 1107 90-91 -0.4967879115301024 1107 92-93 -0.8269291170354869 1107 94-95 -0.9402623992274961 1107 96-97 -0.8694104157940394 1107 98-99 -0.6163164268535688 1107 100-101 -0.4742179303538663 1108 1 0.06520996716969307 1108 2 0.08032202070047134 1108 3 0.06974664033003819 1108 4 0.12555782997325338 1108 5 0.07434542150493684 1108 6 0.11783321475611075 1108 7 0.03974256197155768 1108 8 0.1367906067210498 1108 9 0.19098780103613677 1108 10-11 0.11251180035945652 1108 12-13 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-0.5694208841812376 2316 60-61 -0.6497482926704343 2316 62-63 -0.5645136646257711 2316 64-65 -0.5564201827979893 2316 66-67 -0.6802735849957848 2316 68-69 -0.7923003630088843 2316 70-71 -0.7262019518950922 2316 72-73 -0.8661824493466028 2316 74-75 -0.8077702488408569 2316 76-77 -0.9018613413771632 2316 78-79 -0.6800425284851244 2316 80-81 -0.8766577779818583 2316 82-83 -0.6715238883165426 2316 84-85 -0.7496009249304372 2316 86-87 -0.6103032905694619 2316 88-89 -0.8762256655457215 2316 90-91 -0.8254582357761286 2316 92-93 -1.006829221422155 2316 94-95 -0.9829081966526267 2316 96-97 -1.1306370400586836 2316 98-99 -1.0740701714218197 2316 100-101 -0.7012265645069178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 25.0 5 63.0 6 194.0 7 361.0 8 702.0 9 1120.0 10 1955.0 11 3172.0 12 4664.0 13 6918.0 14 7604.0 15 10057.0 16 12782.0 17 17701.0 18 15502.0 19 8680.0 20 7316.0 21 7111.0 22 7205.0 23 7511.0 24 7979.0 25 8463.0 26 9458.0 27 10912.0 28 12520.0 29 14431.0 30 16977.0 31 19926.0 32 23572.0 33 29501.0 34 39616.0 35 62513.0 36 75463.0 37 104802.0 38 75270.0 39 5776.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.10909542470099 19.71872607780539 14.379960294069102 17.792218203424518 2 14.500235955919358 24.802337923368416 39.016604711906446 21.68082140880578 3 22.542648444493917 31.75935318289641 29.544189605083478 16.153808767526197 4 12.420688238734085 17.49899267987915 37.46478299110579 32.61553609028097 5 11.508377610997272 41.53075030846729 30.928725634219724 16.032146446315718 6 25.52447278354298 36.47047572162532 19.256668302418667 18.748383192413037 7 23.40541616823265 38.80306852442105 21.1505869109529 16.640928396393402 8 25.26327506816069 30.01766925721704 21.923362144517906 22.79569353010437 9 28.772982184936136 18.610102048975357 22.761828760282896 29.855087005805615 10-11 23.233113473631732 28.348026358079174 26.01355217918226 22.40530798910683 12-13 29.276328682676134 26.470648181101264 27.020323565333136 17.23269957088947 14-15 24.736269016969562 26.461681497165728 31.232317657766277 17.56973182809843 16-17 17.99803395272707 30.444963218924226 31.840245332597505 19.716757495751207 18-19 17.350520749226675 28.851059293135645 37.54670124217857 16.25171871545911 20-21 19.29782340463226 27.14645415478395 35.66414928092237 17.891573159661416 22-23 17.89274901972466 27.91609376151363 34.661140646973884 19.530016571787826 24-25 18.28518705204596 28.123223959970556 36.10144074326957 17.490148244713914 26-27 19.36636224669776 26.717594951593306 37.18080978324775 16.735233018461177 28-29 17.284437413672276 27.6393746932965 38.06306059128515 17.013127301746074 30-31 17.171084503575397 29.996190213395085 36.559292351735806 16.273432931293712 32-33 16.00713982230403 29.963971647662156 35.969402553340935 18.059485976692883 34-35 17.113141667359137 28.527102856488835 36.333333855954194 18.026421620197834 36-37 18.297526013591124 28.434495774237078 36.009741326385395 17.2582368857864 38-39 19.046655895036977 27.36048818844551 36.400347138109694 17.192508778407824 40-41 17.16005466378869 28.744669457352916 36.86834196700258 17.226933911855816 42-43 16.39824270104751 30.09350937680798 35.50910399390402 17.999143928240493 44-45 16.836906675028416 30.13640105044487 33.91745384705836 19.10923842746835 46-47 17.060893968612326 30.139692394525184 32.60508285906903 20.194330777793457 48-49 17.677253065467184 29.99399527461036 32.505867541715595 19.82288411820686 50-51 18.14289365051244 30.71268094526606 32.182114070968645 18.962311333252853 52-53 16.904869785256597 31.086839617390815 32.220447109030445 19.78784348832214 54-55 15.835856206424587 30.263894354460664 32.78235143792008 21.117898001194675 56-57 17.38806988057965 31.020050765798462 30.508630028957512 21.083249324664372 58-59 17.02111147432264 29.602549901736907 31.41455736142239 21.96178126251806 60-61 17.25917970587313 29.377645730770436 31.656423442350505 21.706751121005926 62-63 18.623162502508528 29.12762768412603 31.20680626630544 21.042403547060005 64-65 17.503696120132133 29.647743681319973 31.872470920980632 20.976089277567254 66-67 16.928935721217687 29.719941489203254 31.294653285901752 22.056469503677306 68-69 16.857417897273407 30.446012959586348 30.90483499495946 21.791734148180783 70-71 16.745971895376673 30.368075556064223 29.92054321599295 22.96540933256615 72-73 16.654568373127052 29.31779734757326 29.656366655117104 24.371267624182583 74-75 16.4551425064006 29.875546970906086 30.00034491895189 23.66896560374143 76-77 16.466524151053978 28.864911069157785 31.304882063223822 23.363682716564416 78-79 15.98119251024174 28.506532294604686 31.7566095094155 23.755665685738073 80-81 15.674254456173578 29.838215304245846 30.659524664366135 23.82800557521444 82-83 15.246044798700598 29.32626354002863 30.100841759023943 25.326849902246835 84-85 15.654225021988582 27.937102461310285 30.026213840343292 26.382458676357846 86-87 15.961299283189279 28.042718309472335 30.135397580812857 25.86058482652553 88-89 16.404015011063226 27.07922182220338 30.472678590671652 26.044084576061742 90-91 15.75813875927773 27.185359514962354 30.561367519216187 26.49513420654373 92-93 15.278733708458825 27.62714574863875 30.317591964015545 26.776528578886882 94-95 15.17517963222233 27.33921681448534 30.187620231691465 27.29798332160086 96-97 15.041523538366746 26.844414899871595 30.017198913191738 28.09686264856992 98-99 15.52417489899362 26.426827796077646 29.93912180499223 28.109875499936503 100-101 15.963488732508088 26.22632501895096 30.714586398737598 27.09559984980336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3373.0 1 2506.5 2 2399.0 3 3018.5 4 2313.5 5 2102.0 6 3763.0 7 6525.0 8 8567.0 9 7783.5 10 5504.5 11 4314.5 12 4012.0 13 3864.5 14 3641.5 15 3471.0 16 3393.0 17 3484.0 18 3593.0 19 3693.0 20 3919.0 21 4208.0 22 4490.5 23 4884.0 24 5257.5 25 5684.0 26 6378.0 27 7115.0 28 7650.5 29 8303.0 30 9069.5 31 9663.5 32 10316.5 33 10963.5 34 11871.0 35 12383.5 36 12732.5 37 13437.0 38 13557.0 39 13286.0 40 13273.5 41 13543.0 42 13851.5 43 14247.0 44 14944.0 45 15621.0 46 18348.5 47 24452.5 48 39774.5 49 43597.5 50 35282.0 51 28513.5 52 17116.0 53 10689.5 54 8871.0 55 8312.0 56 8254.0 57 7919.5 58 7627.0 59 7430.0 60 7066.5 61 6727.5 62 5683.0 63 3454.5 64 1671.5 65 1248.5 66 994.5 67 741.0 68 576.5 69 471.5 70 427.5 71 386.5 72 353.5 73 385.0 74 339.5 75 275.0 76 221.0 77 136.5 78 80.5 79 51.0 80 32.5 81 18.0 82 9.5 83 4.5 84 2.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2593163392810948 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0019597667720759886 16-17 4.7034402529823734E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.839067088303955E-5 26-27 9.406880505964747E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0031356268353215817 36-37 0.02124387180930372 38-39 0.00697676970859052 40-41 0.017951463632216058 42-43 0.005330565620046689 44-45 9.406880505964747E-4 46-47 1.567813417660791E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.919533544151977E-4 60-61 0.0017245947594268702 62-63 0.0010974693923625537 64-65 0.0 66-67 0.0 68-69 3.135626835321582E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22098330121928847 78-79 0.0 80-81 0.0012542507341286328 82-83 0.0 84-85 0.0 86-87 0.09932098000881111 88-89 0.3396667769362104 90-91 0.34005873029062555 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.839067088303955E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 637831.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.16968315827211 #Duplication Level Percentage of deduplicated Percentage of total 1 83.04458950280824 39.171869748562344 2 10.065969875687271 9.496172194337605 3 2.92636130637635 4.141066068851992 4 1.2118715096208965 2.286543805494184 5 0.6422229465537945 1.5146726452957207 6 0.3869449062806811 1.0951241177380213 7 0.25834241430853355 0.8530150880493614 8 0.191997440514627 0.7245166749019322 9 0.14784950337006322 0.627661280616639 >10 0.9358136515772282 8.459740511433765 >50 0.0848662312303752 2.7531999938850777 >100 0.07920848186428554 7.9694919629383145 >500 0.01231392365117044 3.9817632696680336 >1k 0.009984262419867922 10.213433146372902 >5k 0.001331234989315723 4.592120295076854 >10k+ 3.3280874732893076E-4 2.119609196777235 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13502 2.1168616765256 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7758 1.2163096494212418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7614 1.1937331362069263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7131 1.1180077481339101 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6749 1.0581172755792678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4776 0.7487876882747938 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4404 0.6904650291378124 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3461 0.5426202238523998 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3249 0.509382579397991 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3237 0.507501203296798 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3094 0.48508147142424873 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3043 0.4770856229941787 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2667 0.418135838490133 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2661 0.4171951504395365 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2575 0.4037119550476537 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2538 0.39791104540230876 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2466 0.3866227887951511 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2193 0.3438214824930115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1827 0.2864395114066265 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1817 0.2848716979889657 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1804 0.2828335405460067 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1733 0.2717020652806151 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1665 0.2610409340405217 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1563 0.24504923718038163 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1476 0.23140926044673277 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1466 0.22984144702907194 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1442 0.22607869482668608 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1385 0.21714215834601958 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1350 0.2116548113842068 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1344 0.21071412333361034 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1258 0.1972309279417275 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1234 0.1934681757393416 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1228 0.19252748768874514 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1091 0.1710484438667923 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1013 0.15881949920903812 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 976 0.1530185895636932 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 975 0.15286180822192713 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 922 0.14455239710832493 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 904 0.1417303329565355 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.14141677027300334 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 879 0.13781079941238353 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 858 0.13451839123529585 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 844 0.13232345245057078 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 843 0.1321666711088047 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 821 0.12871748158995094 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 802 0.12573863609639543 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 797 0.12495472938756505 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 777 0.12181910255224346 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 762 0.11946738242575228 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 752 0.1178995690080915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 682 0.10692487508446595 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 677 0.10614096837563555 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 674 0.10567062435033732 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 665 0.1042595922744426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.05516037947356E-4 0.0 18-19 0.0 0.0 0.0 0.0010974693923625537 0.0 20-21 0.0 0.0 0.0 0.0025085014682572656 0.0 22-23 0.0 0.0 0.0 0.005722518974461887 0.0 24-25 0.0 0.0 0.0 0.012385725999520248 0.0 26-27 0.0 0.0 0.0 0.023987545290210105 0.0 28-29 0.0 0.0 0.0 0.06232058335201644 0.0 30-31 0.0 0.0 0.0 0.1597601872596346 0.0 32-33 0.0 0.0 0.0 0.35213089360661365 0.0 34-35 0.0 0.0 0.0 0.7112385569218178 0.0 36-37 0.0 0.0 0.0 1.2470387924073933 0.0 38-39 0.0 0.0 0.0 1.9411097924058254 0.0 40-41 0.0 0.0 0.0 2.747593014450536 0.0 42-43 0.0 0.0 0.0 3.4513060669675824 0.0 44-45 0.0 0.0 0.0 4.197585253774118 0.0 46-47 0.0 0.0 0.0 5.056355053297818 0.0 48-49 0.0 0.0 0.0 5.954555360275684 0.0 50-51 0.0 0.0 0.0 6.860908297025388 0.0 52-53 0.0 0.0 0.0 7.686597233436443 0.0 54-55 0.0 0.0 0.0 8.510796747100722 0.0 56-57 0.0 0.0 0.0 9.433454943394096 0.0 58-59 0.0 0.0 0.0 10.467035938986974 0.0 60-61 0.0 0.0 0.0 11.717288748900572 0.0 62-63 0.0 0.0 0.0 12.81773698675668 0.0 64-65 0.0 0.0 0.0 13.820432058021638 0.0 66-67 0.0 0.0 0.0 14.915392948915937 0.0 68-69 0.0 0.0 0.0 16.107715053046967 0.0 70-71 0.0 0.0 0.0 17.42412958918585 0.0 72-73 0.0 0.0 0.0 18.742582282767692 0.0 74-75 0.0 0.0 0.0 20.049433157058843 0.0 76-77 0.0 0.0 0.0 21.289651961099413 0.0 78-79 0.0 0.0 0.0 22.6314650746044 0.0 80-81 0.0 0.0 0.0 24.03802888225878 0.0 82-83 0.0 0.0 0.0 25.41660722040791 0.0 84-85 0.0 0.0 0.0 26.97477858555009 0.0 86-87 0.0 0.0 0.0 28.546119583400618 0.0 88-89 0.0 0.0 0.0 29.89710440539892 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGGGC 100 1.3098015E-6 33.211475 8 CGTCACT 175 3.6379788E-12 32.636032 1 TACCTGG 325 0.0 29.196903 2 GTGCAAG 70 0.008308112 27.196692 1 ATAGGAC 70 0.008436387 27.111408 3 GTACTTG 155 1.3848785E-6 24.564754 1 ATGGGGA 765 0.0 24.18763 5 GTTCGGC 50 0.0016641811 23.724342 10-11 CGGGAGT 780 0.0 23.722485 4 GTCGGGA 780 0.0 23.722485 2 CTTAACG 60 1.7244599E-4 23.722483 20-21 GGATATG 165 2.3856173E-6 23.075983 1 ATACAGT 145 2.353228E-5 22.904467 6 GTGTAGC 125 2.2243199E-4 22.845222 1 TGTGTAC 125 2.2721378E-4 22.773584 3 CTAGACA 105 0.0022099952 22.592844 4 CAGGGTA 105 0.0022099952 22.592844 4 TCGGGAG 820 0.0 22.565289 3 TCACTAC 360 0.0 22.404568 3 AGTACTT 8700 0.0 22.306335 12-13 >>END_MODULE