##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939315_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207416 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9727285376374 33.0 31.0 34.0 30.0 34.0 2 31.923979804806297 33.0 31.0 34.0 30.0 34.0 3 31.99251956243747 34.0 31.0 34.0 30.0 34.0 4 35.41556679719334 37.0 35.0 37.0 33.0 37.0 5 35.49430602211665 37.0 35.0 37.0 33.0 37.0 6 35.67070835569514 37.0 35.0 37.0 33.0 37.0 7 35.64654849695548 37.0 35.0 37.0 33.0 37.0 8 35.67704005910142 37.0 35.0 37.0 33.0 37.0 9 37.2067671788348 39.0 37.0 39.0 34.0 39.0 10-11 37.12292573562053 39.0 37.0 39.0 33.5 39.0 12-13 37.042092369158595 39.0 37.0 39.0 33.0 39.0 14-15 37.950740672643064 40.0 38.0 41.0 33.0 41.0 16-17 37.95511571819489 40.0 37.5 41.0 33.0 41.0 18-19 38.091104060241044 40.0 38.0 41.0 33.0 41.0 20-21 38.08070996243217 40.0 38.0 41.0 33.0 41.0 22-23 38.17329776978275 40.0 38.0 41.0 33.5 41.0 24-25 38.05320991273928 40.0 38.0 41.0 33.0 41.0 26-27 37.74103457300549 40.0 37.5 41.0 32.5 41.0 28-29 37.55442159123285 40.0 37.0 41.0 32.5 41.0 30-31 37.46202137457181 40.0 37.0 41.0 33.0 41.0 32-33 37.197793469690644 40.0 36.0 41.0 32.0 41.0 34-35 36.90166893597567 39.0 35.0 41.0 31.0 41.0 36-37 36.50301511657954 39.0 35.0 41.0 30.0 41.0 38-39 35.70249400372366 38.5 34.5 40.0 28.5 41.0 40-41 35.69984040297628 38.0 35.0 40.0 30.0 41.0 42-43 35.98160493152319 39.0 35.0 40.5 30.0 41.0 44-45 35.851347008818834 39.0 35.0 41.0 29.5 41.0 46-47 35.393872120296564 39.0 35.0 41.0 26.5 41.0 48-49 35.05918341317326 39.0 35.0 40.0 24.5 41.0 50-51 33.82963079833297 37.5 33.5 39.5 23.0 40.5 52-53 33.8194768828639 37.5 33.5 39.5 23.0 40.5 54-55 34.395933133236596 38.0 34.0 40.0 23.0 41.0 56-57 34.418682541891116 38.0 34.0 40.0 23.0 41.0 58-59 34.27144579829984 38.0 34.0 40.0 22.0 41.0 60-61 33.79522260761826 38.0 33.5 40.0 21.0 41.0 62-63 33.247664847906606 37.0 33.0 40.0 19.0 41.0 64-65 32.63576182525327 36.0 32.0 39.0 13.0 41.0 66-67 32.15267687358789 36.0 32.0 39.0 12.5 40.0 68-69 31.78277453669655 35.5 31.5 38.0 8.0 40.0 70-71 31.088059956137734 35.0 31.0 37.0 4.5 39.0 72-73 30.443611812333117 35.0 30.5 37.0 2.0 39.0 74-75 29.989010001523916 35.0 30.0 36.0 2.0 37.5 76-77 29.322547489846087 34.0 29.0 35.5 2.0 37.0 78-79 28.883255232662147 34.0 29.0 35.0 2.0 36.0 80-81 28.531624560217853 34.0 29.0 35.0 2.0 36.0 82-83 28.08934617397815 34.0 28.0 35.0 2.0 36.0 84-85 27.662622907100783 34.0 27.0 35.0 2.0 35.0 86-87 27.14045697588901 33.5 26.0 35.0 2.0 35.0 88-89 26.461671867856644 33.0 24.5 35.0 2.0 35.0 90-91 25.829438238353642 32.0 23.0 35.0 2.0 35.0 92-93 25.386413630430603 32.0 20.0 35.0 2.0 35.0 94-95 24.864488295666117 32.0 17.5 35.0 2.0 35.0 96-97 24.023555261815318 31.5 4.0 34.0 2.0 35.0 98-99 23.121290425172432 31.0 2.0 34.0 2.0 35.0 100-101 20.41761455869394 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37025726939206294 1101 2 0.4586121314179188 1101 3 0.5072382709701486 1101 4 0.38885705196960174 1101 5 0.3676319567402615 1101 6 0.41683356678245076 1101 7 0.45702730122946633 1101 8 0.420188637308307 1101 9 0.5441024528164036 1101 10-11 0.6278461332936374 1101 12-13 0.5737773279385934 1101 14-15 0.7729698418418209 1101 16-17 0.8208764609577628 1101 18-19 0.7360971127474869 1101 20-21 0.6776993777735996 1101 22-23 0.8315807894561615 1101 24-25 0.8893314386454207 1101 26-27 0.7779379057190994 1101 28-29 0.8143071414255516 1101 30-31 0.637722265784582 1101 32-33 0.6001568187748205 1101 34-35 0.5890564206650524 1101 36-37 0.5399159169307524 1101 38-39 1.0977883607695915 1101 40-41 0.9769368276644812 1101 42-43 0.9955293891886612 1101 44-45 0.7372052069452906 1101 46-47 0.7677418416251882 1101 48-49 0.7515242105758944 1101 50-51 1.0204722866661697 1101 52-53 1.1042309810596365 1101 54-55 1.0257061259497746 1101 56-57 1.0860025730584724 1101 58-59 0.9954055181193624 1101 60-61 1.2601631314657809 1101 62-63 1.293833755353731 1101 64-65 1.3001057268218474 1101 66-67 1.0279119713684608 1101 68-69 1.074590716937056 1101 70-71 1.0764314426909571 1101 72-73 1.0720557583196637 1101 74-75 0.6710369216573397 1101 76-77 0.7576495086084272 1101 78-79 0.8596392260568244 1101 80-81 0.9734316292729517 1101 82-83 0.8204600967680626 1101 84-85 0.9699818846545156 1101 86-87 0.9419399921414815 1101 88-89 1.06923262739598 1101 90-91 1.245815038632685 1101 92-93 1.2896135313198407 1101 94-95 1.2861306963534638 1101 96-97 1.3364998167260502 1101 98-99 1.487581319928399 1101 100-101 1.0058301475466571 1102 1 0.32074783871353674 1102 2 0.33349802782186444 1102 3 0.5145000022121309 1102 4 0.48564213692184666 1102 5 0.47715835611371915 1102 6 0.343674996503772 1102 7 0.4423063057326928 1102 8 0.4510960273924951 1102 9 0.4708638251052193 1102 10-11 0.5299902801816287 1102 12-13 0.5342641989547658 1102 14-15 0.751905165008445 1102 16-17 0.7310872643601982 1102 18-19 0.6996579281150304 1102 20-21 0.6876878477629376 1102 22-23 0.7395900134646922 1102 24-25 0.7237500432965831 1102 26-27 0.7561118130874291 1102 28-29 0.7696607646770133 1102 30-31 0.7471194420197023 1102 32-33 0.7563697541414953 1102 34-35 0.6795426390391057 1102 36-37 0.81373931197502 1102 38-39 1.2436355990057137 1102 40-41 1.19272598075257 1102 42-43 0.8351325827840625 1102 44-45 0.8836367774197598 1102 46-47 0.6951823968060822 1102 48-49 0.6306457542920398 1102 50-51 0.8006863532791684 1102 52-53 0.8953624449358912 1102 54-55 0.9935321750719694 1102 56-57 0.8538447206861193 1102 58-59 0.8934736974654172 1102 60-61 0.9983766324556171 1102 62-63 0.9959352629570049 1102 64-65 0.8860452051432475 1102 66-67 0.8565390299104578 1102 68-69 0.7771825763144342 1102 70-71 0.8345801145642575 1102 72-73 0.7937887406765753 1102 74-75 0.7998441094224766 1102 76-77 0.6157246494703514 1102 78-79 0.6647546349371076 1102 80-81 0.6729304001303049 1102 82-83 0.8153647022459047 1102 84-85 0.6910361192140897 1102 86-87 0.5647628868267986 1102 88-89 0.6374824587967716 1102 90-91 0.6816899053849177 1102 92-93 0.49967248665736363 1102 94-95 0.7520192685900717 1102 96-97 0.618877609490422 1102 98-99 0.8545802539462812 1102 100-101 0.7949236276519507 1103 1 0.29799942433721327 1103 2 0.24869887348782171 1103 3 0.4358742094002892 1103 4 0.2510332574292491 1103 5 0.21474820812218098 1103 6 0.3465291191253357 1103 7 0.3576974262400938 1103 8 0.33109602739249766 1103 9 0.5218786242595499 1103 10-11 0.5480663901182012 1103 12-13 0.4260083850012748 1103 14-15 0.49706372737630744 1103 16-17 0.46319085844054086 1103 18-19 0.6031674418571029 1103 20-21 0.5762396448031097 1103 22-23 0.5179303940143711 1103 24-25 0.6352405295545154 1103 26-27 0.6857629758781272 1103 28-29 0.5530434285247949 1103 30-31 0.47714058366874923 1103 32-33 0.6177016780315583 1103 34-35 0.5511705460158467 1103 36-37 0.5319105593323101 1103 38-39 1.0242064235300319 1103 40-41 0.911129786249397 1103 42-43 0.7498894538200105 1103 44-45 0.6352858260455534 1103 46-47 0.6315988872923342 1103 48-49 0.4014068536577966 1103 50-51 0.3333713427083467 1103 52-53 0.42861825888937943 1103 54-55 0.543479320949352 1103 56-57 0.5033267502844296 1103 58-59 0.5011058327719695 1103 60-61 0.5194759982061399 1103 62-63 0.5658084130627117 1103 64-65 0.5209289260734806 1103 66-67 0.6098054146884735 1103 68-69 0.4554806735660186 1103 70-71 0.20079575938455818 1103 72-73 0.4286090366596582 1103 74-75 0.3865671538199358 1103 76-77 0.20080921606654556 1103 78-79 0.3452408928652275 1103 80-81 0.07544625636709057 1103 82-83 0.1014746388209602 1103 84-85 0.1735625462753987 1103 86-87 0.3946783202306037 1103 88-89 0.5067213594310189 1103 90-91 0.38006199840817345 1103 92-93 0.3271460595960569 1103 94-95 0.5746514038966275 1103 96-97 0.6017634445855613 1103 98-99 0.7456479072232369 1103 100-101 0.3675029088358812 1104 1 0.3286826642429759 1104 2 0.2988142362305304 1104 3 0.2868507306449075 1104 4 0.2554341023394784 1104 5 0.26948539229636026 1104 6 0.23776398792052333 1104 7 0.21048969206184864 1104 8 0.3777805671601513 1104 9 0.38286083239993474 1104 10-11 0.3520415302595623 1104 12-13 0.4177107978699084 1104 14-15 0.4032608189353297 1104 16-17 0.47068740568239065 1104 18-19 0.4960604469753065 1104 20-21 0.7092573554213502 1104 22-23 0.7232244877669132 1104 24-25 0.6873653975372847 1104 26-27 0.566267890980896 1104 28-29 0.7644497166066841 1104 30-31 0.7069951616198438 1104 32-33 0.6794605536510332 1104 34-35 0.6222161224233531 1104 36-37 0.4886808087433181 1104 38-39 0.9829967395589563 1104 40-41 0.7412601786452129 1104 42-43 0.7662579646657335 1104 44-45 0.5311725755784167 1104 46-47 0.8124141444212682 1104 48-49 0.81464350976308 1104 50-51 0.6410421526847898 1104 52-53 0.640173218674903 1104 54-55 0.6738518126221109 1104 56-57 0.5696956673369868 1104 58-59 0.42095878589569935 1104 60-61 0.47716874335197446 1104 62-63 0.45855762095938246 1104 64-65 0.6486679787991321 1104 66-67 0.6870874847185249 1104 68-69 0.4936125283064605 1104 70-71 0.8231533838869538 1104 72-73 0.7688308255945842 1104 74-75 0.5693667313648234 1104 76-77 0.60804254127574 1104 78-79 0.6262393492792313 1104 80-81 0.6159783873281768 1104 82-83 0.48908126811659614 1104 84-85 0.5661488652290103 1104 86-87 0.6782716259418713 1104 88-89 0.5247626300460162 1104 90-91 0.7504730797307815 1104 92-93 0.868652680767557 1104 94-95 0.8639547591651286 1104 96-97 0.9039419318712838 1104 98-99 0.888563253717674 1104 100-101 0.7780634742758039 1105 1 0.24597145230924156 1105 2 0.3717470018300695 1105 3 0.4401234895319206 1105 4 0.2687337921886055 1105 5 0.3408856001419238 1105 6 0.26672245520101967 1105 7 0.29779615104470025 1105 8 0.35625439843322226 1105 9 0.6514425897058658 1105 10-11 0.5177373074361711 1105 12-13 0.5132708284401915 1105 14-15 0.5409633571582901 1105 16-17 0.5406589785557117 1105 18-19 0.6428548133091851 1105 20-21 0.6934691799738175 1105 22-23 0.6493721875149845 1105 24-25 0.6024536105911196 1105 26-27 0.7813059623034704 1105 28-29 0.6573215033911097 1105 30-31 0.5640677741253555 1105 32-33 0.6260151292861877 1105 34-35 0.5140664736176532 1105 36-37 0.4681158246552286 1105 38-39 1.0142722400664326 1105 40-41 0.7677422913913148 1105 42-43 0.6977380757862761 1105 44-45 0.5448826997600733 1105 46-47 0.8012183854412527 1105 48-49 0.784858108285512 1105 50-51 0.7923100510588199 1105 52-53 0.7162653177129101 1105 54-55 0.8831522950340869 1105 56-57 0.7829092266316096 1105 58-59 0.6823193251619344 1105 60-61 0.9031288159766078 1105 62-63 0.7518018314090753 1105 64-65 0.81712802109611 1105 66-67 0.911753172812702 1105 68-69 0.8005793983706333 1105 70-71 0.78689201606905 1105 72-73 0.9848862888192649 1105 74-75 0.8731241262592633 1105 76-77 0.6848754439563045 1105 78-79 0.6003581285706971 1105 80-81 0.609872418440311 1105 82-83 0.8264643549871487 1105 84-85 0.9376740230339351 1105 86-87 0.9344356217526091 1105 88-89 1.0540393355725222 1105 90-91 1.1303334916208456 1105 92-93 1.242446347543403 1105 94-95 1.456702000358984 1105 96-97 1.6073928152149328 1105 98-99 1.4917832424762203 1105 100-101 1.2914889228218982 1106 1 0.12677412789452802 1106 2 0.28668306321114656 1106 3 0.2993129445781548 1106 4 0.18111230881265072 1106 5 0.19285097492059577 1106 6 0.2492563918526045 1106 7 0.25081995588436 1106 8 0.19022646217510442 1106 9 0.24768890093939433 1106 10-11 0.3479478130430991 1106 12-13 0.3527475154360573 1106 14-15 0.4949688657162241 1106 16-17 0.3488627951994303 1106 18-19 0.3223374023314136 1106 20-21 0.37266256970429623 1106 22-23 0.4343928446072596 1106 24-25 0.4110705690802021 1106 26-27 0.5364151497911749 1106 28-29 0.5741501478923823 1106 30-31 0.39065837023002103 1106 32-33 0.40627875313037265 1106 34-35 0.5137792416680256 1106 36-37 0.51631767395682 1106 38-39 0.8909257911189599 1106 40-41 0.609884726960864 1106 42-43 0.48486969097415766 1106 44-45 0.4540210039111727 1106 46-47 0.5175079782014294 1106 48-49 0.623264561167673 1106 50-51 0.6440432794672475 1106 52-53 0.56861825888938 1106 54-55 0.5803370284592333 1106 56-57 0.7267061969247379 1106 58-59 0.785374607475525 1106 60-61 1.0619661167832177 1106 62-63 0.8277451719559963 1106 64-65 0.9013637086821831 1106 66-67 0.8950425688386687 1106 68-69 0.8936032032102936 1106 70-71 0.7563293562224978 1106 72-73 0.7484904595845592 1106 74-75 0.4930493672586742 1106 76-77 0.6964021014420396 1106 78-79 0.7078891142091024 1106 80-81 0.84362807454891 1106 82-83 0.869419302852581 1106 84-85 0.9091554316508947 1106 86-87 0.9336506522464134 1106 88-89 0.8809506084428769 1106 90-91 0.715714172321217 1106 92-93 0.7319879568292542 1106 94-95 0.8613114829480075 1106 96-97 1.051071744980817 1106 98-99 1.1906874329149382 1106 100-101 1.169716347571061 1107 1 0.08171768805547686 1107 2 0.09966529445424399 1107 3 0.25260639613247804 1107 4 0.13779001418600956 1107 5 0.22002257339654108 1107 6 0.09549717809339597 1107 7 0.11230840085107019 1107 8 0.042938779235250024 1107 9 0.1936804260613556 1107 10-11 0.17539644744825722 1107 12-13 0.32951780351069004 1107 14-15 0.45301786333043736 1107 16-17 0.48733090758058495 1107 18-19 0.30241396110362473 1107 20-21 0.2082912418547025 1107 22-23 0.24895824004221367 1107 24-25 0.28368033299431517 1107 26-27 0.4309022060173575 1107 28-29 0.39890747438884233 1107 30-31 0.3279350144631863 1107 32-33 0.37741502774490954 1107 34-35 0.4369199317652317 1107 36-37 0.4291791916009444 1107 38-39 0.9310450803686905 1107 40-41 0.611518811638426 1107 42-43 0.4117403956709538 1107 44-45 0.4550483158080425 1107 46-47 0.5543630150564667 1107 48-49 0.356419138670077 1107 50-51 0.23859656793356976 1107 52-53 0.39714405741518277 1107 54-55 0.5431476256176566 1107 56-57 0.5074340393917112 1107 58-59 0.5207045223706572 1107 60-61 0.41674053547068723 1107 62-63 0.46765116490546177 1107 64-65 0.5008306459751992 1107 66-67 0.43149665137971027 1107 68-69 0.4486706817560311 1107 70-71 0.5504272090160072 1107 72-73 0.34999314804377235 1107 74-75 0.5577547050074863 1107 76-77 0.46928996954730096 1107 78-79 0.5699665275908607 1107 80-81 0.45938565030648704 1107 82-83 0.2218268091731339 1107 84-85 0.311813979526832 1107 86-87 0.4026635782158614 1107 88-89 0.3915125142221747 1107 90-91 0.5061144144605905 1107 92-93 0.18418537163536897 1107 94-95 0.145425359670579 1107 96-97 0.29876180658392215 1107 98-99 0.49217124874657614 1107 100-101 0.4921630234959977 1108 1 0.10062405776830019 1108 2 0.2221739468016075 1108 3 0.15077157010410502 1108 4 0.08462563279582014 1108 5 0.15482152190516985 1108 6 0.10441768217518899 1108 7 0.18483818870343782 1108 8 0.12577344674733837 1108 9 0.3442246653152665 1108 10-11 0.3667100851328584 1108 12-13 0.4247180624206308 1108 14-15 0.3488819091944819 1108 16-17 0.40380669421766413 1108 18-19 0.42649588760490076 1108 20-21 0.32267659494973344 1108 22-23 0.5089054673281552 1108 24-25 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2149.0 10 2591.0 11 3658.0 12 5346.0 13 7473.0 14 7751.0 15 8754.0 16 11455.0 17 14865.0 18 13019.0 19 9116.0 20 9067.0 21 9889.0 22 11048.0 23 12260.0 24 13427.0 25 14907.0 26 17620.0 27 20200.0 28 23617.0 29 27633.0 30 32877.0 31 39823.0 32 49236.0 33 63336.0 34 85604.0 35 133422.0 36 177362.0 37 222238.0 38 134331.0 39 12297.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.03051697787229 16.147335371121006 13.025450196026314 17.796697454980396 2 11.806453218727443 24.94611255382522 38.06478109002813 25.18265313741921 3 27.716326249537452 29.43094004244636 29.16021591555863 13.69251779245756 4 11.078959379278869 15.004605618024147 40.73847558192606 33.177959420770925 5 11.078595248331371 43.322748617706964 32.31702357575264 13.281632558209019 6 24.45930013713372 36.303375612085674 18.886978265880234 20.35034598490037 7 24.146229012292444 38.80039205800601 19.381496984934625 17.67188194476692 8 21.793747931831508 27.533200583524152 21.193471097889578 29.479580386754765 9 25.385137886620385 23.809918746791382 23.087124306544464 27.717819060043773 10-11 23.176380296237266 24.184223870887223 26.236149769693213 26.4032460631823 12-13 35.448782342315596 22.496474988692185 23.366357814455675 18.688384854536537 14-15 29.201939567238 23.487480884631076 30.95304834156552 16.357531206565405 16-17 18.485196454776847 27.992214769552206 33.541492210624064 19.981096565046887 18-19 17.579224191337275 33.80298510433435 31.931883491860503 16.685907212467875 20-21 20.390991589611883 28.856710661827073 26.84140298397269 23.910894764588356 22-23 17.952965849304228 28.25778026284571 25.59232368415377 28.196930203696297 24-25 22.518837093422274 28.047551499395713 27.791397730794586 21.642213676387424 26-27 25.571526703877982 22.04754066807813 32.59913155152617 19.78180107651771 28-29 19.340023552513852 26.511676911609218 34.50181953332175 19.646480002555187 30-31 19.141956762298822 31.13738281010218 30.523126081481717 19.197534346117283 32-33 18.551407063773727 29.98250399446499 29.160880292147624 22.30520864961366 34-35 21.340250009245594 25.601285823570745 29.381550609149777 23.67691355803388 36-37 24.306022713988376 24.910295180339446 32.52188173566189 18.26180037001028 38-39 26.372120936372283 22.706824078976208 33.084948830740736 17.836106153910777 40-41 19.68569883977211 27.11178109535493 34.91878404790618 18.283736016966774 42-43 18.024296879971814 31.171373821478205 29.909653074628036 20.894676223921948 44-45 19.90027070331283 30.114495688213673 26.686030744043116 23.29920286443038 46-47 20.403990823684545 28.790710540491638 25.65795138549468 25.147347250329137 48-49 22.640851647677326 27.248881627912102 27.806725999774518 22.303540724636054 50-51 23.235762110084302 29.119779024370967 29.862285425467523 17.78217344007721 52-53 19.309651081376906 30.23928118711625 30.507238508641322 19.943829222865524 54-55 15.991238498032988 28.175399745606978 32.27534509020637 23.558016666153666 56-57 21.62247010110032 30.544571211399212 25.91775754718001 21.915201140320455 58-59 21.619728206232647 27.242905597275247 27.778327511240203 23.359038685251907 60-61 20.66160317772996 25.264748492049442 31.682712407625818 22.390935922594778 62-63 25.415975575410172 24.875873978235358 29.186827395044613 20.52132305130986 64-65 22.06805203514215 28.34967595556604 31.289059948740285 18.293212060551525 66-67 19.509976943321842 27.27440276047764 31.2991701759247 21.916450120275815 68-69 18.04524542717114 31.62370011246656 29.492327683376047 20.838726776986256 70-71 20.083435162175174 30.471974081595377 25.530560510047046 23.914030246182406 72-73 19.922719398529274 27.08477901641963 26.77983349059037 26.212668094460724 74-75 20.38186165461457 30.004039785437886 27.60080429590201 22.013294264045534 76-77 22.825354979782883 24.97617662118561 29.761358583549868 22.43710981548164 78-79 20.274745155776262 25.53312455030548 32.824227149279864 21.367903144638394 80-81 19.893017125827146 30.76685061366265 29.377661939274617 19.962470321235585 82-83 18.64210866508084 28.89336344159191 28.404887586915134 24.05964030641212 84-85 19.698828180079186 26.73939744839155 28.044774809511154 25.51699956201811 86-87 22.16875458061339 26.76779440917678 29.19846232812041 21.864988682089418 88-89 23.49623192239969 23.541406424513035 29.963382031113834 22.998979621973444 90-91 20.022849211843646 24.737683392800086 32.22537677068911 23.014090624667162 92-93 18.860074308609565 29.40727858239477 30.308592921127193 21.424054187868474 94-95 18.317798304384496 29.963682024453576 28.02962188781078 23.68889778335115 96-97 17.168610662123395 27.257291803183204 27.570328118141667 28.003769416551734 98-99 20.724150613076297 26.924725080878144 26.570758390691356 25.780365915354196 100-101 22.332504035909505 26.01613199098352 28.229736323351958 23.421627649755017 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5840.0 1 3747.0 2 2615.0 3 3233.5 4 2287.5 5 2123.5 6 3941.0 7 7708.0 8 10850.5 9 10429.5 10 7527.5 11 5336.5 12 4811.0 13 4640.0 14 4366.0 15 4098.0 16 3846.5 17 3521.0 18 3270.0 19 3163.5 20 3165.0 21 3249.5 22 3300.5 23 3420.0 24 3733.5 25 4189.5 26 4814.5 27 5577.5 28 6069.0 29 6783.0 30 8125.5 31 8991.0 32 10097.5 33 11562.0 34 12581.5 35 13072.5 36 13805.5 37 15170.5 38 15454.0 39 15281.0 40 15709.0 41 16079.5 42 16006.0 43 16540.5 44 19728.0 45 23319.5 46 34935.0 47 74089.5 48 220867.0 49 227193.5 50 102412.5 51 63208.0 52 26832.0 53 14694.0 54 11563.5 55 9579.5 56 8090.0 57 6949.0 58 6094.0 59 5508.5 60 5005.0 61 4701.5 62 4037.0 63 2571.0 64 1545.5 65 1312.0 66 1115.5 67 904.0 68 746.0 69 555.5 70 462.0 71 383.5 72 326.0 73 361.0 74 313.0 75 216.0 76 156.5 77 107.5 78 87.5 79 64.5 80 45.0 81 36.5 82 25.0 83 15.0 84 11.5 85 10.5 86 6.5 87 4.0 88 2.0 89 2.5 90 2.0 91 1.5 92 1.5 93 1.0 94 2.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2895439517117547 2 0.1760785015272284 3 0.1764097875131686 4 0.19595566068364179 5 0.20986967209313112 6 0.10717101645166206 7 0.12191324282600198 8 0.13574443273900627 9 0.13905729259840852 10-11 0.1262613713914674 12-13 0.11524611235895499 14-15 0.13085796444638798 16-17 0.10650844447978162 18-19 0.13491621777415572 20-21 0.16088075692222067 22-23 0.16514606399120105 24-25 0.18887442273416952 26-27 0.16961842480139405 28-29 0.16767211963399525 30-31 0.15686391434269548 32-33 0.16547734997714125 34-35 0.3429224061963731 36-37 0.46028046671569695 38-39 0.42905676254083097 40-41 0.4195737011932921 42-43 0.4412315225241342 44-45 0.4440888641528686 46-47 0.4479400637394237 48-49 0.46028046671569695 50-51 0.45332346101095233 52-53 0.4592866087578763 54-55 0.4421839697337123 56-57 0.4097179431115705 58-59 0.39861986258257304 60-61 0.35555268441034404 62-63 0.4565534993738695 64-65 0.4553111769265937 66-67 0.46338627283388656 68-69 0.47419447812518634 70-71 0.4674859369098968 72-73 0.4606531634498798 74-75 0.46541539949777044 76-77 0.4423082019784399 78-79 0.43158281818362515 80-81 0.4279800830865253 82-83 0.4397407355874032 84-85 0.4399063785803733 86-87 0.4421839697337123 88-89 0.4481057067323938 90-91 0.46578809623195316 92-93 0.46968070656675076 94-95 0.4695564743220232 96-97 0.4669890079309865 98-99 0.46446295228819234 100-101 0.4467805627886329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207416.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.4337723003298 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7002619416501 15.582408606316603 2 12.482230842144698 5.350825873413666 3 5.015863428075481 3.2252662382076456 4 2.694261829894161 2.3099277831768537 5 1.6058850134057925 1.7210086858925908 6 1.0765640973096264 1.3844897837066772 7 0.753121005364954 1.1299556900511516 8 0.552075963911586 0.9466456402372819 9 0.41930737305696764 0.8088604882157239 >10 2.3474597977829554 9.213811533653791 >50 0.1811388865433415 2.6344396544029003 >100 0.11752694293608716 4.682681550169568 >500 0.01318784178160714 2.0242985009334897 >1k 0.028702949759968482 13.347533780704374 >5k 0.00659392089080357 10.038023134470018 >10k+ 0.005818165491885503 25.59982305644765 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 52580 4.354754285184228 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 42097 3.4865365375313893 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 31102 2.575914183678202 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26146 2.165450847098266 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 18655 1.5450350169287137 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 18364 1.5209339614515627 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 16749 1.3871772446281978 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16674 1.3809656323918185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14014 1.1606604517415704 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13679 1.1329152504190767 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 12793 1.0595354045333174 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12322 1.0205264796888562 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11406 0.9446619889085452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10950 0.9068953865113598 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10393 0.8607638129691838 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9858 0.8164543123496789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9254 0.7664301284727054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8487 0.7029060406686677 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8316 0.6887435647697231 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7951 0.6585137185526777 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7754 0.6421978837451218 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7694 0.6372285939560185 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7014 0.5809099763461806 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6730 0.5573886713444248 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6563 0.5435574814314205 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6428 0.532376579405938 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6325 0.5238459652679772 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5976 0.4949412629946928 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5899 0.4885640077653435 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5833 0.48309778899732986 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5561 0.4605703419533947 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5098 0.42222398908081393 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4541 0.37609241553863787 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4347 0.3600250452205371 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4259 0.3527367535298522 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4091 0.3388227421203628 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4054 0.33575834675041577 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4018 0.33277677287695373 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3877 0.3210989418725609 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3863 0.3199394409217701 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3527 0.29211141810279145 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3450 0.2857341628734421 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3366 0.27877715716869744 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3345 0.27703790574251125 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3215 0.266271111199454 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3120 0.25840306903337373 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 3083 0.2553386736634267 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3058 0.2532681362513003 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3053 0.252854028768875 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3039 0.25169452781808427 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3034 0.251280420335659 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2986 0.2473049885043763 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2978 0.24664241653249586 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.22610268540420203 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2640 0.21864875072054704 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2595 0.21492178337871953 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2551 0.21127763753337708 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2482 0.20556295427590823 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2431 0.20133905795517038 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2366 0.19595566068364179 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2353 0.19487898122933606 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2223 0.1841121866862788 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2181 0.18063368383390646 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2142 0.1774036454709893 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2060 0.17061228275921472 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2016 0.16696813691387227 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1930 0.15984548821615746 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1907 0.1579405937970012 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 0.15446209094462887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.1534682329868082 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1793 0.14849894319770485 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1769 0.1465112272820635 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1759 0.14568301231721295 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1682 0.13930575708786366 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1603 0.13276285886554426 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1595 0.13210028689366382 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1546 0.12804203356589608 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1531 0.12679971111862026 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1506 0.12472917370649386 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1496 0.12390095874164331 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1457 0.12067092037872613 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1445 0.11967706242090546 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1441 0.11934577643496526 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1425 0.11802063249120436 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1397 0.1157016305896228 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1388 0.11495623712125731 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1342 0.11114644828294473 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1295 0.10725383794814712 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1286 0.10650844447978162 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1256 0.10402379958522996 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1247 0.10327840611686445 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1215 0.10062811822934266 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1212 0.1003796537398875 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.282149648505569E-5 0.0 12-13 0.0 0.0 0.0 8.282149648505569E-5 0.0 14-15 0.0 0.0 0.0 2.070537412126392E-4 0.0 16-17 0.0 0.0 0.0 3.3128598594022276E-4 0.0 18-19 0.0 0.0 0.0 4.969289789103341E-4 0.0 20-21 0.0 0.0 0.0 0.0012423224472758354 0.0 22-23 0.0 0.0 0.0 0.0027331093840068377 0.0 24-25 0.0 0.0 0.0 0.00517634353031598 0.0 26-27 0.0 0.0 0.0 0.008406381893233152 0.0 28-29 0.0 0.0 0.0 0.017351103513619168 0.0 30-31 0.0 0.0 0.0 0.037269673418275064 0.0 32-33 0.0 0.0 0.0 0.0773138669687995 0.0 34-35 0.0 0.0 0.0 0.1537995189727484 0.0 36-37 0.0 0.0 0.0 0.2562082993765198 0.0 38-39 0.0 0.0 0.0 0.37186851921790004 0.0 40-41 0.0 0.0 0.0 0.5067847369920557 0.0 42-43 0.0 0.0 0.0 0.6379325766761414 0.0 44-45 0.0 0.0 0.0 0.7740497061493304 0.0 46-47 0.0 0.0 0.0 0.9664440424841149 0.0 48-49 0.0 0.0 0.0 1.1816971118487745 0.0 50-51 0.0 0.0 0.0 1.3877155843553508 0.0 52-53 0.0 0.0 0.0 1.581145189396198 0.0 54-55 0.0 0.0 0.0 1.783395283812704 0.0 56-57 0.0 0.0 0.0 2.025275464297309 0.0 58-59 0.0 0.0 0.0 2.3595844348592365 0.0 60-61 0.0 0.0 0.0 2.8399491144725593 0.0 62-63 0.0 0.0 0.0 3.226021520337647 0.0 64-65 0.0 0.0 0.0 3.5288583222352528 0.0 66-67 0.0 0.0 0.0 3.8738512658437525 0.0 68-69 0.0 0.0 0.0 4.284066137934233 0.0 70-71 0.0 0.0 0.0 4.781906153305902 0.0 72-73 0.0 0.0 0.0 5.3222335963744065 0.0 74-75 0.0 0.0 0.0 5.849019724767603 0.0 76-77 0.0 0.0 0.0 6.292073320214408 0.0 78-79 0.0 0.0 0.0 6.843043325581241 0.0 80-81 0.0 0.0 0.0 7.466150854386558 0.0 82-83 0.0 0.0 0.0 8.097416300595652 0.0 84-85 0.0 0.0 0.0 9.049697867180821 0.0 86-87 0.0 0.0 0.0 10.041485287589364 0.0 88-89 0.0 0.0 0.0 10.767664168770333 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATAGCG 20 0.002088247 71.128006 5 TAGCGTA 30 1.3163766E-4 63.185562 7 GATAGTC 85 1.8189894E-12 55.75197 7 GTTCGAC 55 1.6552804E-9 38.781807 16-17 GCTTATG 355 0.0 38.77177 1 TATAACG 50 0.0016291754 37.93651 2 AGATAGT 125 1.382432E-10 37.91134 6 GTAAGCG 155 0.0 36.808983 94-95 TAGTCAA 135 3.1650416E-10 35.10018 9 CTGGTCG 125 5.7880243E-9 34.11737 9 CGTCACT 60 0.0039665354 31.641335 1 CGACCGT 70 1.7391358E-8 30.478373 18-19 ACCGTCT 70 1.7391358E-8 30.478373 20-21 TTCGACC 70 1.7429556E-8 30.471418 16-17 CGATCCG 55 2.7154438E-6 30.25524 70-71 TATGTAC 480 0.0 29.637897 4 AAGTACT 615 0.0 28.529488 4 GACCGTC 75 3.4013283E-8 28.446482 20-21 TCGACCG 75 3.4013283E-8 28.446482 18-19 AGTACTC 605 0.0 28.216068 5 >>END_MODULE