##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939315_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207416 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.150645676386596 33.0 31.0 34.0 31.0 34.0 2 32.306290458301035 34.0 31.0 34.0 30.0 34.0 3 32.41710976167286 34.0 31.0 34.0 30.0 34.0 4 35.866861959755376 37.0 35.0 37.0 35.0 37.0 5 35.84295139372014 37.0 35.0 37.0 35.0 37.0 6 35.96754142731254 37.0 35.0 37.0 35.0 37.0 7 35.92836354661525 37.0 35.0 37.0 35.0 37.0 8 35.96751906550849 37.0 35.0 37.0 35.0 37.0 9 37.56342718665315 39.0 37.0 39.0 35.0 39.0 10-11 37.4692599733646 39.0 37.0 39.0 34.5 39.0 12-13 37.395908700895134 39.0 37.0 39.0 34.0 39.0 14-15 38.27600263703645 40.0 37.5 41.0 34.0 41.0 16-17 38.34349304630716 40.0 37.5 41.0 33.5 41.0 18-19 38.74273945351064 40.0 38.0 41.0 34.0 41.0 20-21 38.74643950386611 40.0 38.0 41.0 34.0 41.0 22-23 38.73785008646564 40.0 38.0 41.0 34.5 41.0 24-25 38.624450893478304 40.0 38.0 41.0 34.0 41.0 26-27 38.17016049149589 40.0 37.5 41.0 33.5 41.0 28-29 37.95359718605684 40.0 37.0 41.0 33.0 41.0 30-31 37.86677623950652 40.0 37.0 41.0 33.0 41.0 32-33 37.74959872984953 40.0 36.0 41.0 33.0 41.0 34-35 37.64834282467683 40.0 36.0 41.0 33.0 41.0 36-37 37.356930420004375 40.0 35.5 41.0 33.0 41.0 38-39 37.04381050110318 39.0 35.0 41.0 31.5 41.0 40-41 36.82266882333843 39.0 35.0 41.0 31.0 41.0 42-43 36.77516903867433 39.0 35.0 41.0 31.0 41.0 44-45 36.553420693447826 39.0 35.0 41.0 30.5 41.0 46-47 36.08986215190125 39.0 35.0 41.0 30.0 41.0 48-49 35.72077767728769 39.0 35.0 41.0 28.0 41.0 50-51 35.47090812114466 39.0 35.0 40.0 27.0 41.0 52-53 35.00519000907724 38.0 34.5 40.0 26.5 41.0 54-55 34.88790565968978 38.0 34.0 40.0 26.0 41.0 56-57 34.74143045975869 38.0 34.0 40.0 25.5 41.0 58-59 34.55311342569587 38.0 34.0 40.0 25.5 41.0 60-61 34.00022030518065 37.5 33.5 40.0 22.5 41.0 62-63 33.490918622910414 37.0 33.0 39.0 22.0 41.0 64-65 32.98069016809451 36.0 32.0 39.0 22.0 40.0 66-67 32.50939775520616 36.0 32.0 39.0 20.5 40.0 68-69 32.110943535616556 35.0 32.0 38.0 20.0 39.5 70-71 31.470604580360042 35.0 31.0 37.0 16.5 39.0 72-73 30.803537057650388 34.5 30.5 36.5 12.5 38.5 74-75 30.28225234716121 34.0 30.0 36.0 7.5 37.0 76-77 28.18329432440849 31.5 27.0 34.0 4.0 36.0 78-79 29.037909883586103 33.5 29.0 35.0 2.0 36.0 80-81 29.17247659464509 34.0 29.0 35.0 2.0 36.0 82-83 28.892413633743466 34.0 29.0 35.0 2.0 35.5 84-85 28.520280499844297 34.0 29.0 35.0 2.0 35.0 86-87 27.910698549629956 33.0 27.5 35.0 2.0 35.0 88-89 27.11662633259788 33.0 25.5 35.0 2.0 35.0 90-91 26.421055377765413 32.0 25.0 34.0 2.0 35.0 92-93 26.191383085862704 32.0 25.0 34.0 2.0 35.0 94-95 25.790739894120996 32.0 24.0 34.0 2.0 35.0 96-97 24.811477982733372 32.0 19.0 34.0 2.0 35.0 98-99 23.81704979890941 31.0 2.0 34.0 2.0 35.0 100-101 19.75194133587761 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1592671182621217 1101 2 0.19238994610206106 1101 3 0.2743591476850398 1101 4 0.21843263255334477 1101 5 0.1982016076642381 1101 6 0.1856872172245403 1101 7 0.3025673685407071 1101 8 0.27240274963729405 1101 9 0.442129633943118 1101 10-11 0.3932113312231209 1101 12-13 0.3956471012362499 1101 14-15 0.46460840143333115 1101 16-17 0.34351406810663576 1101 18-19 0.32422364670168946 1101 20-21 0.4272996697707754 1101 22-23 0.3241677920897743 1101 24-25 0.33629770838746964 1101 26-27 0.30892405442290993 1101 28-29 0.384740702959391 1101 30-31 0.3234876508136253 1101 32-33 0.36562690934544406 1101 34-35 0.36596753488095857 1101 36-37 0.542652653514395 1101 38-39 0.6616039786269994 1101 40-41 0.6715363926138593 1101 42-43 0.703539794010176 1101 44-45 0.7112308817016313 1101 46-47 0.6346777418341176 1101 48-49 0.7100418205140144 1101 50-51 0.7781779123694932 1101 52-53 0.6458659483615534 1101 54-55 0.5708588161498866 1101 56-57 0.7640123057950063 1101 58-59 0.7936224810003125 1101 60-61 0.8727980345748705 1101 62-63 0.9267417533653841 1101 64-65 1.060278288165371 1101 66-67 0.9970775835343844 1101 68-69 1.1740039884978444 1101 70-71 1.1131663442485866 1101 72-73 1.2748532226414788 1101 74-75 1.240932804880881 1101 76-77 1.0968623526999899 1101 78-79 1.3271139750555712 1101 80-81 1.2361446271113365 1101 82-83 1.1960839128679375 1101 84-85 1.1087711486310248 1101 86-87 1.047607184224404 1101 88-89 1.0899727999226236 1101 90-91 1.3433638903843388 1101 92-93 1.0937708606072434 1101 94-95 0.7467393202764931 1101 96-97 0.7099889660452696 1101 98-99 0.7524857154318347 1101 100-101 0.9540465071603492 1102 1 0.2281112890803172 1102 2 0.301083574139561 1102 3 0.264762698058135 1102 4 0.2848663785015262 1102 5 0.289030245600145 1102 6 0.2768352147009949 1102 7 0.23042866033699738 1102 8 0.34070305202813955 1102 9 0.3377138574077776 1102 10-11 0.3733127082979806 1102 12-13 0.3645617574026261 1102 14-15 0.39099863790732314 1102 16-17 0.3357493673430483 1102 18-19 0.35482777575079183 1102 20-21 0.40704470425203 1102 22-23 0.5218087083992984 1102 24-25 0.48892633327053403 1102 26-27 0.37858446297686754 1102 28-29 0.44961332897370454 1102 30-31 0.42175901940414207 1102 32-33 0.5522542724537587 1102 34-35 0.7122259376481566 1102 36-37 0.6439823030455898 1102 38-39 0.5123055688982845 1102 40-41 0.6936975081967773 1102 42-43 0.74479939328792 1102 44-45 0.8783163995731442 1102 46-47 0.8331270641740574 1102 48-49 0.8781472004605035 1102 50-51 0.9342781581980191 1102 52-53 0.8364130799994669 1102 54-55 0.6988519416529684 1102 56-57 0.7083684743507135 1102 58-59 0.963865046318908 1102 60-61 0.9326957873105286 1102 62-63 0.9626810141394131 1102 64-65 0.8327246083515973 1102 66-67 0.7716140988606028 1102 68-69 0.7483930067065572 1102 70-71 0.5880900938950653 1102 72-73 0.6704039438110101 1102 74-75 0.7692948907250745 1102 76-77 1.0237677413483688 1102 78-79 0.849862468540028 1102 80-81 1.0725490041644399 1102 82-83 0.9887055762351373 1102 84-85 1.0057254854378677 1102 86-87 0.8297395835543604 1102 88-89 1.0098279133735772 1102 90-91 0.9228926827788833 1102 92-93 0.7876399405994121 1102 94-95 0.8586317648563018 1102 96-97 0.590394474344702 1102 98-99 0.37726566626612623 1102 100-101 0.6436768941771227 1103 1 0.1589992383403569 1103 2 0.10302860585203888 1103 3 0.19590434710676163 1103 4 0.16750908463260572 1103 5 0.1210598439088173 1103 6 0.17909737114919722 1103 7 0.21256396689091162 1103 8 0.14317662496682715 1103 9 0.2818787622703596 1103 10-11 0.18016260258973915 1103 12-13 0.14528057347028067 1103 14-15 0.2901318302963247 1103 16-17 0.16491427220562827 1103 18-19 0.1341301013321825 1103 20-21 0.17086077190531057 1103 22-23 0.20937741875870586 1103 24-25 0.17119906054326606 1103 26-27 0.2674745264018128 1103 28-29 0.2350255911301531 1103 30-31 0.21801694752253553 1103 32-33 0.20378704200978603 1103 34-35 0.2278707579441388 1103 36-37 0.2692148611851195 1103 38-39 0.2732146598073726 1103 40-41 0.18161505576548365 1103 42-43 0.1992179979390798 1103 44-45 0.46647707610591027 1103 46-47 0.36412072167934184 1103 48-49 0.3137708791074374 1103 50-51 0.14932044149612267 1103 52-53 -0.04095478469398017 1103 54-55 0.25629380211808694 1103 56-57 0.20297735384331617 1103 58-59 0.07238513088550036 1103 60-61 0.2973575209257504 1103 62-63 0.04419264204639006 1103 64-65 0.11918338213595092 1103 66-67 0.045779003723176004 1103 68-69 -0.19008479456194038 1103 70-71 -0.029055783483368458 1103 72-73 0.21844834127401214 1103 74-75 0.05489742772295969 1103 76-77 0.1484717582616888 1103 78-79 -0.15029609382783704 1103 80-81 0.14632278851962255 1103 82-83 0.3412531449454974 1103 84-85 0.20324134167465147 1103 86-87 0.09163176114421034 1103 88-89 0.3103775962488413 1103 90-91 0.5116664671340629 1103 92-93 0.2175870864767866 1103 94-95 -0.08844011675046204 1103 96-97 -0.16856958485191598 1103 98-99 -0.3861064267571308 1103 100-101 -0.21641824324359504 1104 1 0.039870958220120656 1104 2 0.0697033966555125 1104 3 0.09744387103234686 1104 4 0.06931345541821088 1104 5 0.037349550627475026 1104 6 0.15488663104033407 1104 7 0.09442683146154707 1104 8 0.147163138899721 1104 9 0.15163991264812893 1104 10-11 0.151991345785774 1104 12-13 0.2004077162113589 1104 14-15 0.1669431065982181 1104 16-17 0.08862090962539781 1104 18-19 0.01769578497830082 1104 20-21 0.10324479985234802 1104 22-23 0.19997941729636182 1104 24-25 0.2524115879622215 1104 26-27 0.3880493090811541 1104 28-29 0.13286457073856894 1104 30-31 0.21283618547850125 1104 32-33 0.2067381347061925 1104 34-35 0.3022781021638181 1104 36-37 0.3550548969313212 1104 38-39 0.10622871456405392 1104 40-41 0.2448399318723915 1104 42-43 0.14928055379252214 1104 44-45 0.13554523761375492 1104 46-47 0.2835682803762296 1104 48-49 0.3961699367075653 1104 50-51 0.31383299328749814 1104 52-53 0.6157659074824764 1104 54-55 0.576463596577419 1104 56-57 0.40642280026463595 1104 58-59 0.3608756662661179 1104 60-61 0.6067128706698384 1104 62-63 0.6127315160410305 1104 64-65 0.6423440775620577 1104 66-67 0.6033238895790518 1104 68-69 0.5936360600972463 1104 70-71 0.4630548672599879 1104 72-73 0.4790312480901626 1104 74-75 0.6129110762728018 1104 76-77 0.630994708948176 1104 78-79 0.7464960907525544 1104 80-81 0.9619020394742073 1104 82-83 0.904963032019996 1104 84-85 1.1256456799637107 1104 86-87 0.7842197049251851 1104 88-89 0.7925662387055787 1104 90-91 0.7077774428428931 1104 92-93 0.46962177654097204 1104 94-95 0.19359944779580118 1104 96-97 -0.07750044844765824 1104 98-99 0.22328141953419944 1104 100-101 0.297453314153433 1105 1 0.10229417869412316 1105 2 0.14358804641148026 1105 3 0.16223507520219727 1105 4 0.08766128537303786 1105 5 0.002585964846701927 1105 6 0.19465064140835153 1105 7 0.12492513513443271 1105 8 0.12356741065173082 1105 9 0.21635428674588297 1105 10-11 0.16077140555147906 1105 12-13 0.15401771867801273 1105 14-15 0.3177459808516545 1105 16-17 0.20452554328748818 1105 18-19 0.25806675291590153 1105 20-21 0.35296073076997914 1105 22-23 0.28913060674718594 1105 24-25 0.3756231658497242 1105 26-27 0.4232314165704665 1105 28-29 0.4643139498302773 1105 30-31 0.43375944032384695 1105 32-33 0.2862119700229542 1105 34-35 0.30340346053566947 1105 36-37 0.3381967616376045 1105 38-39 0.41321054627385223 1105 40-41 0.5809836283693528 1105 42-43 0.4375890386630701 1105 44-45 0.35756716249012044 1105 46-47 0.6156715238183779 1105 48-49 0.5537338573057085 1105 50-51 0.7377675825903154 1105 52-53 0.6386523728543523 1105 54-55 0.6523571505343781 1105 56-57 0.5871772715726422 1105 58-59 0.6611017084256119 1105 60-61 0.8124726998644576 1105 62-63 0.7351654927251232 1105 64-65 0.867770383370015 1105 66-67 0.7226527182439497 1105 68-69 0.9050530088111586 1105 70-71 0.8400968286104238 1105 72-73 0.7642669344455477 1105 74-75 0.7996979213469828 1105 76-77 0.9199752547651876 1105 78-79 0.982523733403756 1105 80-81 1.2350661040276414 1105 82-83 1.1349162465497749 1105 84-85 1.2493643363270586 1105 86-87 1.1912759404942719 1105 88-89 1.3040592087539835 1105 90-91 1.3202514116013582 1105 92-93 1.5353596080728416 1105 94-95 1.7270164854708447 1105 96-97 1.673894421729738 1105 98-99 1.27450443297138 1105 100-101 1.0256446938193413 1106 1 0.18457236087000695 1106 2 0.08579540427101051 1106 3 0.22977786489332175 1106 4 0.1654932743559243 1106 5 0.17485431031197862 1106 6 0.14506575059583326 1106 7 0.18003432262213437 1106 8 -0.05045973866953801 1106 9 0.17401314962213377 1106 10-11 0.20166457887432188 1106 12-13 0.1344505339445874 1106 14-15 0.22198953780620911 1106 16-17 0.2324834421661066 1106 18-19 0.23586923176696217 1106 20-21 0.22016907230056404 1106 22-23 0.3830928337784698 1106 24-25 0.3993611158792234 1106 26-27 0.2693124710658452 1106 28-29 0.40079634211829784 1106 30-31 0.35623829139604624 1106 32-33 0.22143526335366204 1106 34-35 0.523641508932279 1106 36-37 0.4911713829242501 1106 38-39 0.6101316558548078 1106 40-41 0.7229391664374205 1106 42-43 0.5253049544608217 1106 44-45 0.7956470365802275 1106 46-47 0.569456690058793 1106 48-49 0.6908064522299711 1106 50-51 0.6461386233143003 1106 52-53 0.6348357291400148 1106 54-55 0.6293372803789552 1106 56-57 0.6150722155033961 1106 58-59 0.5696183324665327 1106 60-61 0.7869425011629048 1106 62-63 0.6587578594376922 1106 64-65 0.7990845679067036 1106 66-67 0.7105220867271314 1106 68-69 0.7305871421969456 1106 70-71 0.751062793591732 1106 72-73 0.7321835191396247 1106 74-75 0.6845021708059633 1106 76-77 0.5807444855344137 1106 78-79 0.7889726808757196 1106 80-81 0.7294650810097387 1106 82-83 0.7929725125344298 1106 84-85 0.6625101163782112 1106 86-87 0.7464736583774112 1106 88-89 0.950970481624335 1106 90-91 1.0963304987546145 1106 92-93 1.204879576595367 1106 94-95 1.6322515828542805 1106 96-97 1.4320628262152795 1106 98-99 1.5219370515037376 1106 100-101 1.4142734563611477 1107 1 0.09990832924945181 1107 2 0.14987545269888614 1107 3 0.1250198262222426 1107 4 0.04743537455889424 1107 5 0.07199350484247447 1107 6 0.022922104973929436 1107 7 0.08697019129713368 1107 8 0.16351241530261973 1107 9 0.11268138307298159 1107 10-11 0.0872551496822851 1107 12-13 -0.0073484191587098735 1107 14-15 0.16050857067306623 1107 16-17 0.19762925992061753 1107 18-19 0.22822510542719243 1107 20-21 0.13059459663913486 1107 22-23 0.1668548962361811 1107 24-25 0.057362041706241484 1107 26-27 0.2098946738219638 1107 28-29 0.18097153707609692 1107 30-31 0.23429049379608102 1107 32-33 0.2000401132628582 1107 34-35 0.30626551633890386 1107 36-37 0.42993967690993884 1107 38-39 0.38855454514725807 1107 40-41 0.3975978567482841 1107 42-43 0.34793216665325133 1107 44-45 0.3441511137799509 1107 46-47 0.4141780517366769 1107 48-49 0.3532876686242261 1107 50-51 0.3663269935026747 1107 52-53 0.44567502693583094 1107 54-55 0.5719612877855695 1107 56-57 0.5985465594125259 1107 58-59 0.50364639214677 1107 60-61 0.5987457273139611 1107 62-63 0.5957937886475335 1107 64-65 0.5420867400393092 1107 66-67 0.46435394229811067 1107 68-69 0.6576465731694228 1107 70-71 0.5601415957140112 1107 72-73 0.6558234436491155 1107 74-75 0.601752464577995 1107 76-77 0.32035955514948355 1107 78-79 0.5797899012581595 1107 80-81 0.5468838040806325 1107 82-83 0.5678256265179797 1107 84-85 0.3898711533044654 1107 86-87 0.30537868989113903 1107 88-89 0.7042678501390931 1107 90-91 0.628103813571407 1107 92-93 0.6580907719804756 1107 94-95 0.988648335337988 1107 96-97 0.963641717359387 1107 98-99 1.1400156043649012 1107 100-101 0.8686800368546841 1108 1 0.011858475877019714 1108 2 0.018277872714207888 1108 3 0.04612428845573646 1108 4 0.052597061172193094 1108 5 0.0334938615041267 1108 6 -0.026371837061944348 1108 7 0.029866019676831 1108 8 0.07244348713691551 1108 9 -0.019998070574217763 1108 10-11 0.004788702296480096 1108 12-13 0.11247998696001105 1108 14-15 0.11060103850747538 1108 16-17 0.05973098774814645 1108 18-19 0.07493655294508983 1108 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3555.0 13 4826.0 14 5855.0 15 7868.0 16 10392.0 17 14855.0 18 13934.0 19 9010.0 20 8234.0 21 9050.0 22 9576.0 23 10603.0 24 12226.0 25 14027.0 26 16352.0 27 19299.0 28 23370.0 29 28330.0 30 34203.0 31 41049.0 32 50670.0 33 64798.0 34 90170.0 35 136701.0 36 194522.0 37 242165.0 38 121093.0 39 4875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.26137373636688 16.629038019055216 13.48418018556821 17.625408059009693 2 12.289550577431473 25.759390301271473 37.405914779992976 24.545144341304074 3 27.812452377639524 29.2169393150331 29.45091004260338 13.519698264724006 4 11.331802792078289 15.65251744220716 40.198986927454996 32.816692838259556 5 10.882413352150378 43.68105110417619 32.26601270813042 13.17052283554301 6 24.431264783637122 36.981537432003556 18.120598037461818 20.466599746897508 7 23.975083980997436 38.6909731194551 19.263865974941528 18.070076924605935 8 21.91150357457579 28.168998920007688 20.76053323792297 29.15896426749356 9 25.023438483505274 24.022540698483372 22.683979672291905 28.270041145719453 10-11 23.378437920319094 24.487127883016292 26.002347161210388 26.132087035454227 12-13 35.6057895538903 22.61105534463681 23.283938592829646 18.49921650864325 14-15 28.937947370491614 23.893564904826682 30.851228152997862 16.31725957168384 16-17 18.335219604632567 28.24011094836586 33.46029947610937 19.964369970892204 18-19 17.614434461693403 33.76760785015273 31.863583056709533 16.754374631444342 20-21 20.393882472983627 28.769289126531373 27.086480550199767 23.750347850285237 22-23 18.030736713775532 28.085142154816566 25.86345551160495 28.020665619802955 24-25 22.692427464933377 28.11677996647386 27.972794794834588 21.217997773758174 26-27 25.535285906135876 22.0691634321408 32.97224526271197 19.423305399011358 28-29 19.386234735998197 26.545407713662893 34.54070510909247 19.527652441246428 30-31 19.169076772214382 31.12435978983217 30.66635691426981 19.040206523683633 32-33 18.69529640157162 29.88435634445792 29.39326628104978 22.027080972920686 34-35 21.444952513783715 25.493310849815682 29.72242904813057 23.33930758827003 36-37 24.294737121102 24.922235654026 32.61418655243145 18.16884067244055 38-39 26.235354725000953 22.601636924728297 33.55993857805209 17.60306977221866 40-41 19.76213824622199 26.98851730438254 34.91136193847515 18.337982510920323 42-43 18.046043126142163 31.053953022643892 30.17645599698197 20.723547854231967 44-45 20.055151542508057 29.85347446849658 26.928016014570144 23.16335797442522 46-47 20.55749864793347 28.759511052988586 25.7313789587804 24.951611340297546 48-49 22.852361680567594 27.074568902865504 27.963866562697863 22.109202853869032 50-51 23.26464118414863 29.0958128764237 29.809030193404755 17.83051574602291 52-53 19.467838095368244 30.246530913783133 30.502987996251495 19.782642994597133 54-55 16.06977214149887 28.166472864364895 32.122855751456 23.640899242680234 56-57 21.820470719289055 30.453057480593753 25.82371734480695 21.90275445531024 58-59 21.85007406474878 27.17979574942469 27.667914952051515 23.302215233775016 60-61 20.741299022906517 25.47023270933798 31.459304335942917 22.32916393181258 62-63 25.487544946730644 24.970856835940655 29.184782361128427 20.356815856200278 64-65 22.127405294536384 28.482762133467837 31.034744075046305 18.355088496949474 66-67 19.528163589832783 27.46991494190043 31.101614198987914 21.90030726927887 68-69 18.300785949236932 31.675091840398345 29.15921776951756 20.864904440847166 70-71 20.28291006699848 30.666907953393014 25.3208195521757 23.72936242743281 72-73 20.179333386339092 27.140687219649234 26.5051978771194 26.17478151689227 74-75 20.6564522788156 30.367740324470176 27.11976707253391 21.856040324180302 76-77 22.977568087610454 25.194937822898527 29.573799891649315 22.253694197841703 78-79 20.348902696175465 25.771566339300357 32.46044852883932 21.41908243568486 80-81 20.05036381458066 31.007288714853104 29.078497897780753 19.863849572785487 82-83 18.88201746539718 29.37835841168247 28.020210101572285 23.71941402134807 84-85 19.834713139464775 27.191870904477 27.60523299343391 25.368182962624314 86-87 22.22583788941945 27.26981799007686 28.563872188882083 21.94047193162161 88-89 23.528553676589766 24.079221335150883 29.651604001379788 22.740620986879563 90-91 20.354612078683292 25.198225777052937 31.707363656330873 22.739798487932898 92-93 18.954362042576875 30.067640316179343 29.829031584805897 21.14896605643788 94-95 18.562326488964864 30.674432010177107 27.463028483969072 23.30021301688896 96-97 17.319507112710117 28.415641336540183 26.80037369059214 27.46447786015756 98-99 20.927775838371993 27.813397967309488 25.860671708017417 25.3981544863011 100-101 22.53832991555492 27.20910509420148 27.39350776702567 22.859057223217924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4853.0 1 3196.0 2 2509.5 3 3153.5 4 2285.5 5 2154.5 6 3797.0 7 7581.5 8 10547.0 9 10060.0 10 7379.5 11 5145.5 12 4572.5 13 4308.0 14 4096.0 15 3771.5 16 3350.5 17 3188.5 18 3143.0 19 3105.5 20 3093.5 21 3151.5 22 3268.0 23 3422.5 24 3729.0 25 4203.0 26 4788.5 27 5469.5 28 6019.0 29 6764.5 30 8001.5 31 8933.5 32 10216.0 33 11620.5 34 12618.5 35 13070.5 36 13964.5 37 15448.5 38 15767.5 39 15672.0 40 15894.5 41 16242.0 42 16097.5 43 16435.0 44 19752.5 45 23418.5 46 33776.5 47 75173.5 48 227384.5 49 231481.0 50 104373.0 51 64446.0 52 24809.0 53 12228.0 54 9680.0 55 8418.5 56 7364.0 57 6520.0 58 5894.0 59 5407.5 60 4954.5 61 4588.5 62 3833.5 63 2416.0 64 1398.5 65 1136.5 66 999.0 67 830.5 68 689.5 69 520.0 70 401.0 71 331.0 72 277.5 73 317.0 74 299.0 75 192.0 76 129.5 77 92.0 78 71.0 79 58.0 80 30.0 81 21.0 82 18.5 83 11.0 84 7.0 85 3.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2936850265360075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011180902025482518 16-17 6.211612236379177E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.1410748242527845E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001076679454305724 36-37 0.006832773460017095 38-39 0.002484644894551671 40-41 0.0028159308804918937 42-43 3.726967341827506E-4 44-45 0.0012837331955183632 46-47 4.141074824252785E-4 48-49 2.0705374121263924E-4 50-51 0.0 52-53 1.2423224472758355E-4 54-55 0.0 56-57 7.039827201229734E-4 58-59 0.002194769656853976 60-61 7.039827201229734E-4 62-63 0.0012009116990333076 64-65 1.2423224472758355E-4 66-67 4.969289789103342E-4 68-69 0.001863483670913753 70-71 4.1410748242527845E-5 72-73 0.0 74-75 1.2423224472758355E-4 76-77 0.2479261497280142 78-79 1.6564299297011138E-4 80-81 1.2423224472758355E-4 82-83 0.0 84-85 0.0 86-87 0.10315417387213685 88-89 0.3586999012767762 90-91 0.2625855546058691 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.2423224472758355E-4 100-101 3.3128598594022276E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207416.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.92807997699899 #Duplication Level Percentage of deduplicated Percentage of total 1 70.62880869265942 14.074965483074783 2 13.318022654622855 5.308052411936174 3 5.389375118303468 3.221996951507997 4 2.956869097261696 2.3569889540699154 5 1.756129732204045 1.7498146876674014 6 1.1391368425370807 1.362048606169503 7 0.818129681606012 1.1412627608620929 8 0.5925348775618697 0.9446465943371396 9 0.47880687283322654 0.8587531489821592 >10 2.541834291511369 9.300582274390983 >50 0.19537213701283618 2.6667247116608515 >100 0.12553699004408286 4.761038020709192 >500 0.014133303514896745 2.0355810850625846 >1k 0.03159209020976919 13.592824020951225 >5k 0.0070666517574483724 10.080819150470886 >10k+ 0.006650966359951409 26.543901138147113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 53341 4.417781444009355 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 43651 3.615241143069166 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28825 2.38732963618173 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28786 2.384099597818813 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 19683 1.6301755153153512 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 18245 1.511078203369841 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 16730 1.3856036361949815 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15612 1.2930092031246894 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 14408 1.1932921213566823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13813 1.1440133309480742 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 12692 1.051170433388327 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12505 1.0356828135456215 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10975 0.9089659239234861 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 10529 0.8720275364911514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10341 0.8564570951519609 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10295 0.8526473063136484 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9523 0.7887091110271853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9029 0.7477952917635677 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8890 0.736283103752145 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 8354 0.6918907816361552 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8334 0.6902343517064541 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7812 0.647001530541255 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7529 0.6235630470359843 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6947 0.5753609360816819 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6820 0.5648426060280798 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6658 0.5514255235975007 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6619 0.5481954852345836 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6613 0.5476985562556733 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6228 0.5158122801089268 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6115 0.5064534510061156 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5878 0.4868247563391573 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5185 0.42942945927501375 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5159 0.4272761003664023 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4638 0.38412610069768827 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4253 0.3522398245509418 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4219 0.34942389367044996 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4160 0.34453742537783166 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4073 0.3373319551836318 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3966 0.32847005505973087 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3962 0.32813876907379064 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3769 0.31215422025217493 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3667 0.3037064276106992 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3458 0.2863967348453226 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3411 0.2825041245105249 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3403 0.2818415525386445 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3320 0.27496736833038493 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3237 0.26809318412212524 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3221 0.26676804017836436 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3152 0.26105335692089554 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3050 0.2526055642794199 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2994 0.24796756047625676 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 2989 0.24755345299383147 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2942 0.24366084265903384 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.22891861628469395 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.22196161057994926 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2673 0.22138186010455385 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2611 0.2162469273224804 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2454 0.20324395237432666 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2446 0.2025813804024462 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2430 0.20125623645868532 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2383 0.19736362612388772 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2293 0.1899096914402327 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2247 0.18609990260192014 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2235 0.18510604464409947 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.17152331922055034 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2021 0.16738224439629754 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1993 0.165063242494716 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1920 0.15901727325130693 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1870 0.15487619842705413 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1864 0.1543792694481438 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1826 0.15123205258171168 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1802 0.14924433666607034 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1730 0.14328118891914635 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1721 0.14253579545078085 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1716 0.14212168796835556 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1683 0.13938857858434872 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1632 0.1351646822636109 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1632 0.1351646822636109 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1624 0.13450211029173043 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1623 0.1344192887952454 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1622 0.13433646729876034 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1593 0.1319346439006937 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1592 0.13185182240420867 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1586 0.13135489342529832 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1566 0.12969846349559722 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1426 0.11810345398768941 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1365 0.11305134270210102 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1356 0.11230594923373552 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1350 0.11180902025482518 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1313 0.10874462488487811 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1257 0.10410662108171499 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1221 0.101125047208253 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1211 0.10029683224340245 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.282149648505569E-5 0.0 12-13 0.0 0.0 0.0 8.282149648505569E-5 0.0 14-15 0.0 0.0 0.0 1.2423224472758352E-4 0.0 16-17 0.0 0.0 0.0 2.4846448945516704E-4 0.0 18-19 0.0 0.0 0.0 4.141074824252784E-4 0.0 20-21 0.0 0.0 0.0 0.0011595009507907796 0.0 22-23 0.0 0.0 0.0 0.00269169863576431 0.0 24-25 0.0 0.0 0.0 0.00517634353031598 0.0 26-27 0.0 0.0 0.0 0.008530614137960735 0.0 28-29 0.0 0.0 0.0 0.017723800247801916 0.0 30-31 0.0 0.0 0.0 0.03776660239718539 0.0 32-33 0.0 0.0 0.0 0.07789361744419487 0.0 34-35 0.0 0.0 0.0 0.15516607366475182 0.0 36-37 0.0 0.0 0.0 0.25885858726404154 0.0 38-39 0.0 0.0 0.0 0.37592677254566775 0.0 40-41 0.0 0.0 0.0 0.512292366508312 0.0 42-43 0.0 0.0 0.0 0.6450552253738562 0.0 44-45 0.0 0.0 0.0 0.783035838517959 0.0 46-47 0.0 0.0 0.0 0.977128015530687 0.0 48-49 0.0 0.0 0.0 1.194037514825048 0.0 50-51 0.0 0.0 0.0 1.4018780602542953 0.0 52-53 0.0 0.0 0.0 1.5963843447494483 0.0 54-55 0.0 0.0 0.0 1.7992556003895923 0.0 56-57 0.0 0.0 0.0 2.0423781033214734 0.0 58-59 0.0 0.0 0.0 2.379337361770922 0.0 60-61 0.0 0.0 0.0 2.8623109185235247 0.0 62-63 0.0 0.0 0.0 3.2512820767655883 0.0 64-65 0.0 0.0 0.0 3.5551127366210156 0.0 66-67 0.0 0.0 0.0 3.9009338951943655 0.0 68-69 0.0 0.0 0.0 4.31218403599091 0.0 70-71 0.0 0.0 0.0 4.809692765376639 0.0 72-73 0.0 0.0 0.0 5.352049335109026 0.0 74-75 0.0 0.0 0.0 5.881609983634473 0.0 76-77 0.0 0.0 0.0 6.328431957171348 0.0 78-79 0.0 0.0 0.0 6.882424947159885 0.0 80-81 0.0 0.0 0.0 7.507106084398417 0.0 82-83 0.0 0.0 0.0 8.140980407746792 0.0 84-85 0.0 0.0 0.0 9.095622386981786 0.0 86-87 0.0 0.0 0.0 10.086126074194809 0.0 88-89 0.0 0.0 0.0 10.81284329510293 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 5.4139073E-4 47.454323 2 GTTCGAC 50 6.4392225E-10 42.708893 16-17 TAGTCAA 115 5.638867E-11 41.26634 9 GTAAGCG 210 0.0 39.546906 94-95 GATAGTC 130 2.0736479E-10 36.503323 7 GACCGTC 55 7.077688E-8 34.512234 20-21 CGACCGT 55 7.077688E-8 34.512234 18-19 AGATAGT 170 3.6379788E-12 33.49717 6 GGCGCGA 60 0.003969681 31.636217 8 AGTACTC 550 0.0 31.061012 5 ATGGGCG 140 1.7222192E-8 30.50635 5 TTCGACC 70 1.7236744E-8 30.50635 16-17 GTCGGGA 220 0.0 30.198206 2 TAGGGTC 95 3.5139885E-5 29.971151 4 CCTCTAG 255 0.0 29.849428 1 ACCACTC 575 0.0 29.711763 94-95 AAGTACT 560 0.0 29.65895 4 GCGACTG 40 4.5204881E-4 29.65895 48-49 CGATCCG 50 4.904063E-5 28.543518 70-71 TCGACCG 70 5.8596015E-7 27.116755 18-19 >>END_MODULE