##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939314_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1225294 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93605371445547 33.0 31.0 34.0 30.0 34.0 2 31.898117513021365 33.0 31.0 34.0 30.0 34.0 3 31.96573557040188 34.0 31.0 34.0 30.0 34.0 4 35.40015947193082 37.0 35.0 37.0 33.0 37.0 5 35.49118252435742 37.0 35.0 37.0 33.0 37.0 6 35.66851629078409 37.0 35.0 37.0 33.0 37.0 7 35.63274120333569 37.0 35.0 37.0 33.0 37.0 8 35.65947029855692 37.0 35.0 37.0 33.0 37.0 9 37.18833602384407 39.0 37.0 39.0 34.0 39.0 10-11 37.112228575345995 39.0 37.0 39.0 33.5 39.0 12-13 37.03387391107766 39.0 37.0 39.0 33.0 39.0 14-15 37.949821430611756 40.0 38.0 41.0 33.0 41.0 16-17 37.95820064409031 40.0 37.5 41.0 33.0 41.0 18-19 38.1127974184155 40.0 38.0 41.0 33.0 41.0 20-21 38.01032609316621 40.0 38.0 41.0 33.0 41.0 22-23 38.002543471199566 40.0 38.0 41.0 33.0 41.0 24-25 38.01669436070037 40.0 38.0 41.0 33.0 41.0 26-27 37.71417308825474 40.0 37.5 41.0 32.5 41.0 28-29 37.54423224140492 40.0 37.0 41.0 32.5 41.0 30-31 37.45173770539968 40.0 37.0 41.0 33.0 41.0 32-33 37.2087898904263 39.5 36.0 41.0 32.0 41.0 34-35 36.89293712366175 39.0 35.0 41.0 31.0 41.0 36-37 36.4832101520125 39.0 35.0 41.0 30.0 41.0 38-39 35.97080741438381 38.5 35.0 40.0 30.0 41.0 40-41 35.8516298945396 38.0 35.0 40.0 30.0 41.0 42-43 35.82156608944466 38.0 35.0 40.0 30.0 41.0 44-45 35.7441103930975 39.0 35.0 41.0 29.0 41.0 46-47 35.35970101869429 39.0 35.0 41.0 26.5 41.0 48-49 35.073162441014155 39.0 35.0 41.0 24.5 41.0 50-51 33.83245082404713 38.0 33.5 39.5 23.0 40.5 52-53 33.83011832262298 37.5 33.5 39.5 23.0 40.5 54-55 34.42690121717727 38.0 34.0 40.0 23.0 41.0 56-57 34.435293488746375 38.0 34.0 40.0 23.0 41.0 58-59 34.289319134836205 38.0 34.0 40.0 22.0 41.0 60-61 33.82753567715177 38.0 33.5 40.0 21.5 41.0 62-63 33.305788651539956 37.0 33.0 40.0 19.5 41.0 64-65 32.731163704384414 36.0 32.0 39.0 14.5 41.0 66-67 32.25729457583242 36.0 32.0 39.0 12.5 40.0 68-69 31.87392046317047 35.5 32.0 38.0 8.0 40.0 70-71 31.195467781609963 35.0 31.0 37.0 6.0 39.0 72-73 30.552363351163066 35.0 30.5 37.0 2.0 39.0 74-75 30.0989207488162 35.0 30.0 36.0 2.0 37.5 76-77 29.450050355261673 34.0 29.5 35.5 2.0 37.0 78-79 28.98858886112231 34.0 29.0 35.0 2.0 36.5 80-81 28.587010546040382 34.0 29.0 35.0 2.0 36.0 82-83 28.13914170803089 34.0 28.0 35.0 2.0 36.0 84-85 27.719043756029166 34.0 27.0 35.0 2.0 35.0 86-87 27.30905480643829 34.0 26.0 35.0 2.0 35.0 88-89 26.662199031416137 33.0 25.0 35.0 2.0 35.0 90-91 26.059280466565575 33.0 23.5 35.0 2.0 35.0 92-93 25.66945769749954 33.0 21.5 35.0 2.0 35.0 94-95 25.171615138897277 32.0 18.5 35.0 2.0 35.0 96-97 24.396148597805915 32.0 6.0 35.0 2.0 35.0 98-99 23.559981114736544 32.0 2.0 34.5 2.0 35.0 100-101 21.078534213013366 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26079696034835465 1101 2 0.179563736963253 1101 3 0.34750159153862725 1101 4 0.3821101706186738 1101 5 0.2682388744852062 1101 6 0.352769966294872 1101 7 0.2876877239257354 1101 8 0.2691772245745483 1101 9 0.43256627388642954 1101 10-11 0.3513930305118791 1101 12-13 0.4158326555924674 1101 14-15 0.4450447061669749 1101 16-17 0.4883837245363125 1101 18-19 0.5280604874552921 1101 20-21 0.5233347099839847 1101 22-23 0.46439462778018736 1101 24-25 0.5366872755818406 1101 26-27 0.5878085346727602 1101 28-29 0.4833250274603529 1101 30-31 0.5147565128270841 1101 32-33 0.6318653043066362 1101 34-35 0.5920703010784436 1101 36-37 0.5855672115731494 1101 38-39 0.6897059327649018 1101 40-41 0.6608400675145631 1101 42-43 0.592119328454892 1101 44-45 0.6406417878620445 1101 46-47 0.7145540976631892 1101 48-49 0.6626805097294906 1101 50-51 0.5208792333478627 1101 52-53 0.6393605305055488 1101 54-55 0.6881919804431291 1101 56-57 0.5829833096300163 1101 58-59 0.583663763914231 1101 60-61 0.7455701979902543 1101 62-63 0.7543996447335743 1101 64-65 0.5376092589214565 1101 66-67 0.4964770021966558 1101 68-69 0.44350941808653843 1101 70-71 0.4387102308039772 1101 72-73 0.5374176357065394 1101 74-75 0.47520386064376297 1101 76-77 0.4164856527560161 1101 78-79 0.48775231995931634 1101 80-81 0.38575030015931944 1101 82-83 0.45862555726047205 1101 84-85 0.5915610820992505 1101 86-87 0.5298557304349458 1101 88-89 0.38191549641516787 1101 90-91 0.44671173536274367 1101 92-93 0.5244178793523098 1101 94-95 0.597507704531612 1101 96-97 0.39092258878912745 1101 98-99 0.17452099125831566 1101 100-101 0.2971665127131864 1102 1 0.36404677669487384 1102 2 0.4278530388079531 1102 3 0.497855124487419 1102 4 0.3024122125732873 1102 5 0.24931576407545464 1102 6 0.37081307044680045 1102 7 0.4543991702989203 1102 8 0.476204861022282 1102 9 0.37863081788164266 1102 10-11 0.5512237462162872 1102 12-13 0.5518174293585858 1102 14-15 0.6833788840795805 1102 16-17 0.7148896830714406 1102 18-19 0.7344925254647592 1102 20-21 0.6091157644270311 1102 22-23 0.5602460147040702 1102 24-25 0.6871887516116644 1102 26-27 0.7393949266300979 1102 28-29 0.7042431588202049 1102 30-31 0.8279107783491995 1102 32-33 0.6493540470285168 1102 34-35 0.530040062873077 1102 36-37 0.7390445541753152 1102 38-39 0.9366816020798083 1102 40-41 0.8397538422057593 1102 42-43 0.8692213955768366 1102 44-45 0.6793935223671284 1102 46-47 0.7069864689697098 1102 48-49 0.8572171588017028 1102 50-51 0.7045199595273246 1102 52-53 0.7762508179572478 1102 54-55 0.7878813849977391 1102 56-57 0.7996136423829157 1102 58-59 0.7939202155929621 1102 60-61 0.7445256688233641 1102 62-63 0.8972357135968281 1102 64-65 0.7921100445502276 1102 66-67 0.7598105849359555 1102 68-69 0.5232260815789509 1102 70-71 0.6651575222552495 1102 72-73 0.7384212679642523 1102 74-75 0.6900537749388036 1102 76-77 0.6189385320344627 1102 78-79 0.43444179593472043 1102 80-81 0.5116855725106255 1102 82-83 0.5019975371810261 1102 84-85 0.4548493466418897 1102 86-87 0.583106737128098 1102 88-89 0.653732849628426 1102 90-91 0.8016157083996909 1102 92-93 0.6681012199410894 1102 94-95 0.4691454004286548 1102 96-97 0.1428428729758835 1102 98-99 0.1293566894136191 1102 100-101 0.0646029652081559 1103 1 0.30942613700252863 1103 2 0.38341717053798874 1103 3 0.40484481953670937 1103 4 0.32402586494031027 1103 5 0.4358340177857798 1103 6 0.3350318035201383 1103 7 0.4711802611883158 1103 8 0.40101494389521264 1103 9 0.6246987547248892 1103 10-11 0.6298827823445521 1103 12-13 0.49268837373081453 1103 14-15 0.7900904741023353 1103 16-17 0.7977355285919714 1103 18-19 0.6767700752968437 1103 20-21 0.7793395687277567 1103 22-23 0.7934437654894637 1103 24-25 0.755804353929939 1103 26-27 0.7541634376256638 1103 28-29 0.6804171919693829 1103 30-31 0.6895209438242276 1103 32-33 0.7081792691063029 1103 34-35 0.777292019594988 1103 36-37 0.8514731236694146 1103 38-39 1.01955960825466 1103 40-41 1.0226252469831323 1103 42-43 0.8872305156613365 1103 44-45 1.1614664965844383 1103 46-47 1.1456027822077175 1103 48-49 1.03406690422473 1103 50-51 0.9395855672621352 1103 52-53 1.1164301389950566 1103 54-55 1.0729999731788666 1103 56-57 1.0281252473011264 1103 58-59 1.141384771166571 1103 60-61 1.117750908504867 1103 62-63 1.2150058292397716 1103 64-65 0.9659155301422615 1103 66-67 0.8603989556433476 1103 68-69 1.0554087405541388 1103 70-71 0.9648051091905714 1103 72-73 1.1921742470564425 1103 74-75 1.1594689926833617 1103 76-77 1.2836053138024184 1103 78-79 1.2222288720911543 1103 80-81 1.173186222494376 1103 82-83 1.2294021385243248 1103 84-85 1.2163687852184246 1103 86-87 1.338614672346381 1103 88-89 1.441858458988193 1103 90-91 1.5805814556101794 1103 92-93 1.3636887781688039 1103 94-95 1.2237519909930583 1103 96-97 1.086061545113246 1103 98-99 0.9872421433189373 1103 100-101 0.6838876784028223 1104 1 0.2313874629693835 1104 2 0.3437635519493796 1104 3 0.424436528325554 1104 4 0.3286103247969052 1104 5 0.2624571908589317 1104 6 0.2388707988573131 1104 7 0.28996185281874176 1104 8 0.27773411634142775 1104 9 0.3568262183822668 1104 10-11 0.38369336970398393 1104 12-13 0.41465704658845937 1104 14-15 0.4366805371876339 1104 16-17 0.2916523030130307 1104 18-19 0.3180311935915867 1104 20-21 0.3903559865797206 1104 22-23 0.40416875667319374 1104 24-25 0.4860975438519688 1104 26-27 0.5099870730631437 1104 28-29 0.6390835843521216 1104 30-31 0.651339152358382 1104 32-33 0.8340315084386205 1104 34-35 0.7642488394688272 1104 36-37 0.902210751505315 1104 38-39 1.0004136108407593 1104 40-41 0.990492395605834 1104 42-43 0.9697018138079656 1104 44-45 1.114123132296207 1104 46-47 1.2001191608762625 1104 48-49 1.2746139047341174 1104 50-51 1.371075810591158 1104 52-53 1.4157057355625966 1104 54-55 1.2663603430703247 1104 56-57 1.2195852214400773 1104 58-59 1.312155900824493 1104 60-61 1.3784332877219825 1104 62-63 1.3635771038763451 1104 64-65 1.339490233327865 1104 66-67 1.2245451489743289 1104 68-69 1.4412160169147832 1104 70-71 1.3820497312665054 1104 72-73 1.1790110677139403 1104 74-75 1.1326969226234098 1104 76-77 1.5195923440912011 1104 78-79 1.5601197266814886 1104 80-81 1.2893503618306106 1104 82-83 1.4162458162799005 1104 84-85 1.4817090932000845 1104 86-87 1.3937163533149963 1104 88-89 1.3260837048641356 1104 90-91 1.2620447603396165 1104 92-93 1.4751386625777165 1104 94-95 1.3693624686389185 1104 96-97 1.4813095761465114 1104 98-99 1.3764559280452424 1104 100-101 1.237595899083832 1105 1 0.3153481951807784 1105 2 0.393996294161461 1105 3 0.3381460183028757 1105 4 0.43245229880264446 1105 5 0.4583893846662903 1105 6 0.31421284510422964 1105 7 0.39983989087413363 1105 8 0.33090204879907503 1105 9 0.4466463378628802 1105 10-11 0.4540704058057159 1105 12-13 0.4704535369238414 1105 14-15 0.5802166797602979 1105 16-17 0.5535548214031252 1105 18-19 0.42521432182280705 1105 20-21 0.5573508794429216 1105 22-23 0.6012721212272751 1105 24-25 0.45903183893516797 1105 26-27 0.6846638723394776 1105 28-29 0.5941346557201186 1105 30-31 0.5882942026445193 1105 32-33 0.5584542072431802 1105 34-35 0.6183303086658114 1105 36-37 0.7215536395189304 1105 38-39 0.7886629559588343 1105 40-41 0.8479326867063506 1105 42-43 0.8776916874801444 1105 44-45 1.023821666742684 1105 46-47 0.807593216872597 1105 48-49 0.7697894507831506 1105 50-51 0.83227405607294 1105 52-53 0.9100752428777952 1105 54-55 0.9583019973303593 1105 56-57 1.0089990628487087 1105 58-59 0.9426328810178504 1105 60-61 0.9764889008610851 1105 62-63 1.0856625651833482 1105 64-65 0.9137384539963804 1105 66-67 0.799315547682788 1105 68-69 0.8115497868360713 1105 70-71 0.7618765409078776 1105 72-73 1.0888175713040127 1105 74-75 1.065969636190303 1105 76-77 1.0288352880132052 1105 78-79 0.9665465997694547 1105 80-81 1.0090381229196304 1105 82-83 0.8111572894344512 1105 84-85 0.9464726761109645 1105 86-87 0.9452651412337616 1105 88-89 1.05227984592916 1105 90-91 1.1429669660196602 1105 92-93 1.1702129924623215 1105 94-95 1.4485018878198908 1105 96-97 1.4782039909616067 1105 98-99 1.3143842710840197 1105 100-101 1.0618256668441681 1106 1 0.2237402359825289 1106 2 0.2580732486757711 1106 3 0.16025517241396514 1106 4 0.21113894297385372 1106 5 0.3498353887349239 1106 6 0.25544409910182964 1106 7 0.3045309683011439 1106 8 0.23057342877528697 1106 9 0.2783208718620642 1106 10-11 0.3642384836292649 1106 12-13 0.45220509836272527 1106 14-15 0.46239888080039293 1106 16-17 0.5224772120796146 1106 18-19 0.6832718174130932 1106 20-21 0.4914836826492035 1106 22-23 0.6594247996399929 1106 24-25 0.5854347030797271 1106 26-27 0.5768687562133934 1106 28-29 0.598003879231868 1106 30-31 0.5443456423891604 1106 32-33 0.594479728089965 1106 34-35 0.6375791923756466 1106 36-37 0.6933330341470665 1106 38-39 0.8828950896189056 1106 40-41 0.9586771029766155 1106 42-43 0.9256036665384002 1106 44-45 0.8901836167067572 1106 46-47 0.85651019891624 1106 48-49 1.0675137988985597 1106 50-51 1.081905251122528 1106 52-53 0.7385535939132666 1106 54-55 0.9599116733137478 1106 56-57 1.0462855851737558 1106 58-59 1.2214384087621681 1106 60-61 1.1994297842002126 1106 62-63 1.2172738971807249 1106 64-65 1.2763660164501331 1106 66-67 0.8971863542831429 1106 68-69 0.9112498052193452 1106 70-71 1.030703065086442 1106 72-73 0.934601648349684 1106 74-75 0.9648289616000909 1106 76-77 0.9604506460996411 1106 78-79 1.0695757380192745 1106 80-81 1.0762665935983051 1106 82-83 1.017868476036842 1106 84-85 1.2615437707665969 1106 86-87 1.4109085482470576 1106 88-89 1.4442370873440957 1106 90-91 1.3249302389717847 1106 92-93 1.2646574453472077 1106 94-95 1.3305454546613262 1106 96-97 1.7053783449262419 1106 98-99 1.6444677402662045 1106 100-101 1.5961769423547594 1107 1 0.1966078486443088 1107 2 0.13917413749535967 1107 3 0.23798790280536153 1107 4 0.16371963843619852 1107 5 0.34180482581584926 1107 6 0.23227241658194941 1107 7 0.30094450330815903 1107 8 0.19937118333633208 1107 9 0.2612617731563134 1107 10-11 0.2649869632799735 1107 12-13 0.2642665360673817 1107 14-15 0.36791112156135597 1107 16-17 0.3638930860855396 1107 18-19 0.5141466030048676 1107 20-21 0.3164330043395296 1107 22-23 0.3177516357541421 1107 24-25 0.3917266101435075 1107 26-27 0.287448827942697 1107 28-29 0.41033820014251887 1107 30-31 0.4066487766476925 1107 32-33 0.36428325218165725 1107 34-35 0.34371516617886044 1107 36-37 0.5080999139225213 1107 38-39 0.594254827651028 1107 40-41 0.36413476709636683 1107 42-43 0.45927745415729504 1107 44-45 0.5063305058381999 1107 46-47 0.5172540005691317 1107 48-49 0.7087971296330124 1107 50-51 0.3452999182050078 1107 52-53 0.4696232960807336 1107 54-55 0.611876432396862 1107 56-57 0.557894816422781 1107 58-59 0.43719546474220294 1107 60-61 0.07165027383064881 1107 62-63 0.2765753161733997 1107 64-65 0.294656987914351 1107 66-67 0.22379636519847423 1107 68-69 0.2339926712726772 1107 70-71 0.24536079157950752 1107 72-73 0.16460320768229053 1107 74-75 0.43436739914881883 1107 76-77 0.3288429741832246 1107 78-79 0.5222577901009835 1107 80-81 0.5660061850531655 1107 82-83 0.6370014871081047 1107 84-85 0.4254140342938051 1107 86-87 0.275355764838352 1107 88-89 0.3197087854573013 1107 90-91 0.3637108408741696 1107 92-93 0.38678437502130336 1107 94-95 0.3818163107349264 1107 96-97 0.3606410924702921 1107 98-99 0.5748279460981109 1107 100-101 0.6140780806675608 1108 1 0.04714050837359096 1108 2 0.11549960906395995 1108 3 0.029859350596471046 1108 4 0.1617139274452839 1108 5 0.23651529505063706 1108 6 0.10984167607509931 1108 7 -0.0522514550675055 1108 8 0.11911391417842765 1108 9 0.21972117844447325 1108 10-11 0.2131212813931498 1108 12-13 0.11736639603827825 1108 14-15 0.10567985388610879 1108 16-17 0.24537629632138902 1108 18-19 0.34397897993026305 1108 20-21 0.49818803577743154 1108 22-23 0.34375105916370785 1108 24-25 0.3396309785024272 1108 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-1.7479081399807654 2316 54-55 -1.7388372661724034 2316 56-57 -1.741529226054773 2316 58-59 -1.4391452888346237 2316 60-61 -1.6274649621064299 2316 62-63 -1.739302671918665 2316 64-65 -1.7771449760063796 2316 66-67 -1.8956290102569646 2316 68-69 -2.098127096789174 2316 70-71 -2.0347456244873214 2316 72-73 -1.944137239309626 2316 74-75 -2.020621825946712 2316 76-77 -1.6718040754924175 2316 78-79 -1.4733894817376374 2316 80-81 -1.5417023149024054 2316 82-83 -1.5872359384672983 2316 84-85 -1.6836063380513444 2316 86-87 -1.447016602530347 2316 88-89 -1.8347633553051637 2316 90-91 -1.891502768829909 2316 92-93 -1.8948138654479045 2316 94-95 -1.7083085448591362 2316 96-97 -2.052785882558588 2316 98-99 -2.2246222005294563 2316 100-101 -1.7405984500353604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3659.0 3 903.0 4 843.0 5 821.0 6 992.0 7 1194.0 8 1593.0 9 2105.0 10 2596.0 11 3815.0 12 5340.0 13 8162.0 14 7718.0 15 9041.0 16 11457.0 17 15508.0 18 12925.0 19 9343.0 20 9298.0 21 9973.0 22 10840.0 23 12236.0 24 13656.0 25 15442.0 26 17711.0 27 20401.0 28 23735.0 29 27892.0 30 33384.0 31 39662.0 32 49120.0 33 62814.0 34 84534.0 35 131511.0 36 174858.0 37 230036.0 38 145430.0 39 14742.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.10085418042628 16.0919342681277 12.9929215229406 17.814290028505418 2 11.87537429950333 24.891233568609252 38.119519295267054 25.11387283662036 3 27.686302579853884 29.457873608159307 29.16543079139324 13.690393020593564 4 11.083936655281178 15.034646750232886 40.70290411675301 33.17851247773292 5 11.132584901374013 43.30881235321325 32.31629285927921 13.242309886133524 6 24.402777335466226 36.29398992509556 18.925354414037905 20.377878325400307 7 24.140944195774157 38.86451185083376 19.404938943073695 17.589605010318387 8 21.848452626541288 27.543280232461274 21.185712582342287 29.42255455865515 9 25.371521718003642 23.764241631829062 23.183510536214637 27.68072611395265 10-11 23.219730934379935 24.171235751722698 26.244881726436343 26.364151587461027 12-13 35.441683762135604 22.500890162156512 23.374351569952047 18.683074505755837 14-15 29.187543562294234 23.508306591988255 30.98066660977453 16.32348323594298 16-17 18.486229710213276 27.954572273554483 33.57522037894276 19.983977637289478 18-19 17.54333167684718 33.82412690453373 31.938958553409837 16.693582865209258 20-21 20.422314645236504 28.818666454325133 26.886358485596507 23.872660414841857 22-23 17.990876717251744 28.269475799206102 25.619884999264908 28.119762484277246 24-25 22.503307141669822 28.058488316672037 27.829285734998198 21.608918806659943 26-27 25.582892243350976 22.021825785793638 32.62293341174933 19.772348559106057 28-29 19.30741368910394 26.531689903574218 34.482416415130324 19.678479992191516 30-31 19.12855197806515 31.147512196237525 30.560344933201456 19.163590892495876 32-33 18.588662766362756 29.980125519168237 29.146802054336103 22.284409660132905 34-35 21.331791096115925 25.669046963659127 29.446082980597016 23.553078959627932 36-37 24.303290016522766 24.901876879797385 32.56303527518493 18.231797828494916 38-39 26.356404133576937 22.718830225399728 33.09604388865395 17.828721752369383 40-41 19.65840688114439 27.110510412667278 34.91728889984045 18.313793806347885 42-43 18.03164860646892 31.147126881009036 29.892138287018767 20.929086225503283 44-45 19.869637619770796 30.14766186689509 26.7360802597912 23.246620253542915 46-47 20.3998534075577 28.72192273595548 25.70989847040204 25.16832538608477 48-49 22.619750136881287 27.245539021506644 27.849290940608878 22.285419901003195 50-51 23.21786731290074 29.125127045108893 29.87523576541965 17.78176987657071 52-53 19.259709706942832 30.26246767525998 30.49469393103646 19.98312868676073 54-55 16.012198022980204 28.188971090701916 32.23296455113347 23.56586633518441 56-57 21.644754589787663 30.542407308783236 25.921774765654646 21.89106333577445 58-59 21.639941568650084 27.272410997176255 27.75403168537772 23.33361574879595 60-61 20.708221231580946 25.254642812722977 31.694643009105604 22.34249294659047 62-63 25.416383155202162 24.952857263338547 29.201558023435858 20.429201558023436 64-65 22.11988379531004 28.31985611192605 31.324076599432082 18.236183493331826 66-67 19.46862281442506 27.29721361975521 31.32288150655703 21.911282059262692 68-69 18.053000204792134 31.659799303706738 29.476182674585296 20.81101781691583 70-71 20.05377896558433 30.459455861722933 25.599442959686254 23.88732221300648 72-73 19.90856296677299 27.111072161461223 26.786734422676776 26.193630449089007 74-75 20.36595803932329 29.993611296538226 27.683194024105067 21.95723664003342 76-77 22.812434100210673 24.955131840526093 29.818213831636402 22.41422022762683 78-79 20.215336370968075 25.57852203922725 32.84792585223668 21.35821573756801 80-81 19.918597839182834 30.76710196599073 29.42710220343417 19.887197991392267 82-83 18.704076894920483 28.964280186931042 28.306484037409135 24.02515888073934 84-85 19.649654965705395 26.79552751368529 28.123990416919682 25.430827103689634 86-87 22.21372420569931 26.788404847690796 29.230306256141503 21.76756469046839 88-89 23.446224153216477 23.6218973110541 29.987653739606518 22.944224796122906 90-91 20.011755405643413 24.762618629245974 32.31036728474705 22.915258680363557 92-93 18.84883399942824 29.464685774948663 30.297666196755067 21.388814028868026 94-95 18.305832664708095 29.95781005473102 28.111257601132383 23.6250996794285 96-97 17.177568095273788 27.25690967200485 27.577536311130718 27.987985921590646 98-99 20.66261650627387 27.004841680884823 26.62039862162399 25.71214319121732 100-101 22.37089556547257 26.01550117165732 28.287038231282658 23.326565031587457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5598.0 1 3577.5 2 2572.0 3 3245.0 4 2278.0 5 2125.5 6 3970.0 7 7911.0 8 11079.5 9 10599.0 10 7639.5 11 5325.5 12 4763.5 13 4549.0 14 4326.5 15 4236.5 16 4026.5 17 3637.5 18 3376.5 19 3174.0 20 3155.5 21 3270.0 22 3376.0 23 3555.5 24 3830.0 25 4300.0 26 4965.0 27 5633.5 28 6154.5 29 6969.0 30 8250.5 31 9169.0 32 10334.5 33 11704.0 34 12702.5 35 13189.5 36 14114.5 37 15640.5 38 15893.5 39 15735.5 40 16031.5 41 16434.5 42 16412.0 43 16777.5 44 20095.5 45 23866.0 46 35615.0 47 74980.5 48 224382.5 49 230511.0 50 102998.0 51 63695.0 52 27238.5 53 15106.5 54 11790.0 55 9641.0 56 8144.5 57 7065.5 58 6206.0 59 5630.5 60 5203.5 61 4791.5 62 4015.5 63 2584.5 64 1575.0 65 1311.0 66 1122.0 67 901.5 68 738.0 69 567.5 70 453.5 71 399.5 72 351.0 73 384.0 74 329.0 75 216.5 76 160.0 77 108.5 78 83.5 79 58.0 80 41.5 81 26.0 82 20.5 83 17.0 84 11.5 85 9.0 86 7.5 87 6.0 88 4.0 89 3.5 90 4.0 91 3.5 92 3.5 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2791982985308016 2 0.10944312140596461 3 0.1195631415807145 4 0.12356218181105921 5 0.12894864416213578 6 0.0544359149722434 7 0.06373980448773926 8 0.0679020708499348 9 0.06553529193809812 10-11 0.062025930103305815 12-13 0.0651272266084711 14-15 0.06157705824071611 16-17 0.06165867130664151 18-19 0.06647384219624025 20-21 0.06949352563548014 22-23 0.07785886489283388 24-25 0.08565291268870981 26-27 0.08602017148537412 28-29 0.08034806340355866 30-31 0.07679789503580366 32-33 0.07014643016288336 34-35 0.23076094390407526 36-37 0.34697794978184826 38-39 0.3207801556197941 40-41 0.3062938364180352 42-43 0.3271051682290128 44-45 0.33946954771671123 46-47 0.3445295578040862 48-49 0.35464957797883606 50-51 0.3485694045673936 52-53 0.34983440708923735 54-55 0.33563373361821736 56-57 0.3149856279390905 58-59 0.3011114067317721 60-61 0.26034568030203364 62-63 0.32669710289938575 64-65 0.3546087714458734 66-67 0.3583629724784419 68-69 0.3708497715650285 70-71 0.37186993488909603 72-73 0.3545679649129107 74-75 0.3581997463465911 76-77 0.34412149247445917 78-79 0.32228999733941405 80-81 0.32241241693830214 82-83 0.3342055049645228 84-85 0.3414282612989209 86-87 0.33412389189859737 88-89 0.3490182764299833 90-91 0.37370622887241756 92-93 0.36701395746653454 94-95 0.36770766852690046 96-97 0.3721147740868722 98-99 0.3621171735110104 100-101 0.33955116078263664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1225294.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.22692026068359 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35456949764935 15.358646772226917 2 12.468482819174934 5.293349811486593 3 5.161466763126214 3.2868613022714626 4 2.704796000951 2.2965795613441093 5 1.6712742543206167 1.7738002665098576 6 1.0999306620756188 1.4008884273696038 7 0.7877544281174277 1.1705120301454328 8 0.5850939486908936 0.9935794075096075 9 0.4252291456902492 0.8123674651276972 >10 2.3896035047764483 9.359344794122029 >50 0.1814989229958902 2.645058189296871 >100 0.11469187204560465 4.522042875496972 >500 0.013902045096436926 2.028304189379275 >1k 0.029348761870255733 13.483501297728617 >5k 0.006564854628872993 10.047499571806735 >10k+ 0.005792518790182053 25.527664038178273 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 53723 4.384498740710393 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 42296 3.4519062363808195 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 31472 2.568526410804264 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26524 2.164704960605373 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 18943 1.5459963078248975 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 18381 1.5001297647748215 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 16910 1.3800769447985546 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16859 1.375914678436359 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14195 1.158497470811087 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13750 1.122179656474283 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 12961 1.0577869474591404 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12504 1.0204897763312315 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11460 0.9352857355051114 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11038 0.9008450216845916 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10405 0.8491839509538119 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9936 0.8109074230347981 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9629 0.7858522117956996 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8745 0.7137062615176439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8492 0.6930581558385172 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8191 0.668492622994971 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7837 0.6396015976573786 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7718 0.6298896428122557 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7228 0.5898992405088085 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6737 0.5498272251394359 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6616 0.5399520441624622 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6576 0.5366875215254461 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6316 0.5154681243848415 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6030 0.4921267875301764 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5950 0.48559774225614427 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5860 0.478252566322858 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5553 0.4531973550837595 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5159 0.4210418071091509 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4547 0.3710946107628047 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4403 0.35934232926954673 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4377 0.3572203895554863 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4188 0.3417955200955852 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4107 0.3351848617556276 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4104 0.33494002255785144 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3876 0.3163322435268597 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3773 0.3079260977365432 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3566 0.29103219308998496 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3491 0.28491121314557977 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3415 0.27870862013524916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3393 0.2769131326848903 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3315 0.2705473135427089 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3177 0.25928471044500345 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3126 0.2551224440828079 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3104 0.25332695663244903 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3100 0.25300050436874744 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3094 0.252510825973195 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 3041 0.24818533347914867 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3013 0.24590016763323738 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2926 0.2387998308977274 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.23137304189851576 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2677 0.2184781774823022 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2595 0.2117859060764192 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2589 0.21129622768086678 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2569 0.20966396636235873 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2482 0.20256362962684876 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2468 0.20142104670389313 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2293 0.1871387601669477 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2216 0.1808545540906917 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2204 0.17987519729958687 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2099 0.17130582537741962 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2087 0.17032646858631478 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2086 0.1702448555203894 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2015 0.16445032783968583 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1902 0.15522805139011533 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1878 0.1532693378079057 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1873 0.1528612724782787 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1867 0.15237159408272627 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1832 0.1495151367753372 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1773 0.14469996588573844 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1694 0.13825253367763166 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1664 0.1358041416998696 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1617 0.13196832760137567 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1571 0.12821412656880718 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1536 0.1253576692614181 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1523 0.12429669940438785 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1515 0.12364379487698463 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1514 0.12356218181105921 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1498 0.12225637275625278 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1490 0.12160346822884956 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1424 0.116217005877773 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1401 0.11433990536148875 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1373 0.11205473951557748 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1337 0.109116669142263 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1326 0.10821892541708357 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1263 0.10307730226378321 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1248 0.1018531062749022 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1240 0.10120020174749897 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1239 0.10111858868157356 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1233 0.10062891028602115 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.161306592540239E-5 0.0 10-11 0.0 0.0 0.0 2.04032664813506E-4 0.0 12-13 0.0 0.0 0.0 2.448391977762072E-4 0.0 14-15 0.0 0.0 0.0 2.8564573073890836E-4 0.0 16-17 0.0 0.0 0.0 5.304849285151156E-4 0.0 18-19 0.0 0.0 0.0 6.120979944405179E-4 0.0 20-21 0.0 0.0 0.0 8.569371922167252E-4 0.0 22-23 0.0 0.0 0.0 0.002081133181097761 0.0 24-25 0.0 0.0 0.0 0.00375420103256851 0.0 26-27 0.0 0.0 0.0 0.007263562867360813 0.0 28-29 0.0 0.0 0.0 0.015302449861012948 0.0 30-31 0.0 0.0 0.0 0.036685073133468374 0.0 32-33 0.0 0.0 0.0 0.07471676185470588 0.0 34-35 0.0 0.0 0.0 0.14653625986906 0.0 36-37 0.0 0.0 0.0 0.24300290379288564 0.0 38-39 0.0 0.0 0.0 0.35354780158884314 0.0 40-41 0.0 0.0 0.0 0.49261646592572883 0.0 42-43 0.0 0.0 0.0 0.6223812407471186 0.0 44-45 0.0 0.0 0.0 0.7590015131062422 0.0 46-47 0.0 0.0 0.0 0.9499352808387211 0.0 48-49 0.0 0.0 0.0 1.1648224834203056 0.0 50-51 0.0 0.0 0.0 1.3699977311567673 0.0 52-53 0.0 0.0 0.0 1.5577485893181553 0.0 54-55 0.0 0.0 0.0 1.7541504324676365 0.0 56-57 0.0 0.0 0.0 1.9903794517887134 0.0 58-59 0.0 0.0 0.0 2.3282575447198797 0.0 60-61 0.0 0.0 0.0 2.806306078377924 0.0 62-63 0.0 0.0 0.0 3.187602322381404 0.0 64-65 0.0 0.0 0.0 3.477940804411023 0.0 66-67 0.0 0.0 0.0 3.819205839578093 0.0 68-69 0.0 0.0 0.0 4.22070131739811 0.0 70-71 0.0 0.0 0.0 4.7136850421205025 0.0 72-73 0.0 0.0 0.0 5.240946254531565 0.0 74-75 0.0 0.0 0.0 5.7548229241308615 0.0 76-77 0.0 0.0 0.0 6.1930442816173095 0.0 78-79 0.0 0.0 0.0 6.744952639937844 0.0 80-81 0.0 0.0 0.0 7.360682415812041 0.0 82-83 0.0 0.0 0.0 7.987185116388393 0.0 84-85 0.0 0.0 0.0 8.936630718831562 0.0 86-87 0.0 0.0 0.0 9.926515595440769 0.0 88-89 0.0 0.0 0.0 10.649076874611318 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCCGG 25 0.0050678747 56.867344 8 TATAACG 55 8.291154E-7 51.727154 2 CGTCACT 75 2.386514E-9 50.664593 1 GCTTATG 375 0.0 37.998447 1 GTAAGCG 270 0.0 36.10471 94-95 ATAACGC 75 3.1097292E-4 31.612331 3 TATGTAC 490 0.0 29.999458 4 CGACCGT 40 4.5525056E-4 29.623247 18-19 GTTCGAC 40 4.5557728E-4 29.619617 16-17 TTAGTAC 65 0.0058934675 29.180613 3 AGTACTC 480 0.0 27.660788 5 GTCACTA 160 6.349728E-8 26.671814 2 AAGTACT 500 0.0 26.554358 4 TTATGTA 540 0.0 26.343607 3 GACCGTC 45 9.062219E-4 26.332851 20-21 GTGTGCA 205 9.840733E-10 25.428486 9 CTTATGT 560 0.0 25.401728 2 CCGACGC 95 1.1785232E-8 25.027655 94-95 CATGGGG 2250 0.0 24.657616 4 ACCACTC 655 0.0 24.320766 94-95 >>END_MODULE