##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939314_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1225294 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11510706818119 33.0 31.0 34.0 30.0 34.0 2 32.260899016889006 33.0 31.0 34.0 30.0 34.0 3 32.372537529768366 34.0 31.0 34.0 30.0 34.0 4 35.839402625002656 37.0 35.0 37.0 35.0 37.0 5 35.80212504101056 37.0 35.0 37.0 35.0 37.0 6 35.93138544708454 37.0 35.0 37.0 35.0 37.0 7 35.90102620269094 37.0 35.0 37.0 35.0 37.0 8 35.94321118033713 37.0 35.0 37.0 35.0 37.0 9 37.545669855561194 39.0 37.0 39.0 35.0 39.0 10-11 37.438924862114725 39.0 37.0 39.0 34.5 39.0 12-13 37.3660423539167 39.0 37.0 39.0 34.0 39.0 14-15 38.246482068793284 40.0 37.5 41.0 33.0 41.0 16-17 38.259252473283965 40.0 37.5 41.0 33.5 41.0 18-19 38.665405200711014 40.0 38.0 41.0 34.0 41.0 20-21 38.69965698028392 40.0 38.0 41.0 34.0 41.0 22-23 38.70492469562407 40.0 38.0 41.0 34.0 41.0 24-25 38.58286297003005 40.0 38.0 41.0 34.0 41.0 26-27 38.122734217257246 40.0 37.5 41.0 33.5 41.0 28-29 37.917760145728295 40.0 37.0 41.0 33.0 41.0 30-31 37.827934356978815 40.0 37.0 41.0 33.0 41.0 32-33 37.590260378325524 40.0 36.0 41.0 33.0 41.0 34-35 37.53652755991623 40.0 36.0 41.0 33.0 41.0 36-37 37.297227032859055 39.5 35.0 41.0 32.5 41.0 38-39 37.02205185041305 39.0 35.0 41.0 31.5 41.0 40-41 36.817461768359266 39.0 35.0 41.0 31.0 41.0 42-43 36.77154870586162 39.0 35.0 41.0 31.0 41.0 44-45 36.57071119257909 39.0 35.0 41.0 30.5 41.0 46-47 36.130422576132744 39.0 35.0 41.0 30.0 41.0 48-49 35.767371341082224 39.0 35.0 41.0 28.0 41.0 50-51 35.53919345071469 39.0 35.0 40.0 28.0 41.0 52-53 35.10916522891649 38.0 34.5 40.0 27.0 41.0 54-55 34.9663721523161 38.0 34.0 40.0 26.0 41.0 56-57 34.87942689672846 38.0 34.5 40.0 26.0 41.0 58-59 34.70837733637805 38.0 34.0 40.0 26.0 41.0 60-61 34.17360364124855 38.0 33.5 40.0 24.0 41.0 62-63 33.638248861089664 37.0 33.0 39.0 22.0 41.0 64-65 32.9838659129972 36.0 32.0 39.0 22.0 40.0 66-67 32.52452146178794 36.0 32.0 39.0 21.0 40.0 68-69 32.22578989205856 35.0 32.0 38.0 20.5 39.5 70-71 31.6868571950895 35.0 31.5 37.0 19.0 39.0 72-73 31.017467644500016 35.0 31.0 36.5 15.5 39.0 74-75 30.517572109224396 34.0 30.5 36.0 10.0 37.0 76-77 28.375036929912334 32.5 27.0 34.0 4.5 36.0 78-79 29.268913011897553 34.0 29.0 35.0 3.5 36.0 80-81 29.439961348051977 34.0 29.5 35.0 2.0 36.0 82-83 29.1970824961193 34.0 29.0 35.0 2.0 36.0 84-85 28.896319577179028 34.0 29.0 35.0 2.0 35.0 86-87 28.352139976201627 34.0 29.0 35.0 2.0 35.0 88-89 27.666944015069035 33.0 27.0 35.0 2.0 35.0 90-91 27.066315104783015 33.0 25.5 35.0 2.0 35.0 92-93 26.857511748200842 33.0 25.0 35.0 2.0 35.0 94-95 26.588273916300906 33.0 25.0 35.0 2.0 35.0 96-97 25.837076244558446 32.0 24.0 34.0 2.0 35.0 98-99 25.08055576865634 32.0 19.0 34.0 2.0 35.0 100-101 21.902659688205446 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20730826412567183 1101 2 0.17508649850896774 1101 3 0.23274926638381999 1101 4 0.16502943863584818 1101 5 0.18835165703300305 1101 6 0.18833716033032744 1101 7 0.22357162012639265 1101 8 0.2243417965654757 1101 9 0.20061759556298142 1101 10-11 0.18470177469168192 1101 12-13 0.24980942322356015 1101 14-15 0.28327237317612486 1101 16-17 0.33614701370077427 1101 18-19 0.205006245584336 1101 20-21 0.4161962411832434 1101 22-23 0.34748913625369227 1101 24-25 0.3859258058403512 1101 26-27 0.48891175802516074 1101 28-29 0.4756954964110989 1101 30-31 0.39506459984718134 1101 32-33 0.5338334210400006 1101 34-35 0.44891794040971433 1101 36-37 0.5779613059471203 1101 38-39 0.5605921687879913 1101 40-41 0.5021906704017525 1101 42-43 0.5000018382019462 1101 44-45 0.6824697566547115 1101 46-47 0.6682897113995168 1101 48-49 0.5531714498097458 1101 50-51 0.4647860623360103 1101 52-53 0.36422784625616345 1101 54-55 0.4753573084846039 1101 56-57 0.5070337638863904 1101 58-59 0.4634346389656727 1101 60-61 0.5813662094416756 1101 62-63 0.6239572859652611 1101 64-65 0.4268888282530483 1101 66-67 0.49097235340374823 1101 68-69 0.6177120920793584 1101 70-71 0.6274471553338081 1101 72-73 0.26699698086473944 1101 74-75 -0.00555956343576014 1101 76-77 0.32778441881305653 1101 78-79 0.4424779079494634 1101 80-81 0.28835828137633257 1101 82-83 0.10179339069143367 1101 84-85 0.17202623902396752 1101 86-87 0.38793727613424167 1101 88-89 0.548036334066353 1101 90-91 0.321235309204134 1101 92-93 0.5237768368478974 1101 94-95 0.8802381602883251 1101 96-97 0.47801562374324646 1101 98-99 0.41188923466667404 1101 100-101 0.29118860097637267 1102 1 0.19960332044607298 1102 2 0.303574214131757 1102 3 0.28752561725719517 1102 4 0.23846362267023835 1102 5 0.22323646548922227 1102 6 0.22053263565274506 1102 7 0.1721333412995989 1102 8 0.188177365483142 1102 9 0.2902813056353537 1102 10-11 0.26049582091794576 1102 12-13 0.3495243352059276 1102 14-15 0.28606671188755683 1102 16-17 0.4437756051466053 1102 18-19 0.3499564550853407 1102 20-21 0.32106416372229063 1102 22-23 0.30383827512293493 1102 24-25 0.3234674679723639 1102 26-27 0.30278703350538905 1102 28-29 0.5736576229823598 1102 30-31 0.48941912588516345 1102 32-33 0.47866213016009596 1102 34-35 0.5770872383092609 1102 36-37 0.6191285938613618 1102 38-39 0.5253753386470521 1102 40-41 0.5473052327623122 1102 42-43 0.4773278605396527 1102 44-45 0.578563974290887 1102 46-47 0.41578275637422024 1102 48-49 0.509161359071328 1102 50-51 0.5564716611823926 1102 52-53 0.6258507308401562 1102 54-55 0.7307665836655417 1102 56-57 0.6156044296642591 1102 58-59 0.6906093172319814 1102 60-61 0.48876957030035584 1102 62-63 0.5969495963260414 1102 64-65 0.6387352429290019 1102 66-67 0.6906899861784126 1102 68-69 0.5937618927813233 1102 70-71 0.43585321317784675 1102 72-73 0.6927065975051043 1102 74-75 0.6453406787147529 1102 76-77 0.7702154297234358 1102 78-79 0.7654355282084815 1102 80-81 0.6604857198864238 1102 82-83 0.6558091950936777 1102 84-85 0.7246535891083106 1102 86-87 0.9150325412754476 1102 88-89 1.048486115042575 1102 90-91 1.2182273882975636 1102 92-93 1.1395337952741933 1102 94-95 0.9985722191662738 1102 96-97 0.8817153060907437 1102 98-99 0.8252672853550358 1102 100-101 0.7069486202939999 1103 1 0.1854831005349027 1103 2 0.16745339840215223 1103 3 0.2095952738491107 1103 4 0.1290496913518595 1103 5 0.17358887855390037 1103 6 0.21071855808801843 1103 7 0.14650976313905062 1103 8 0.24095130655128116 1103 9 0.3710688692795969 1103 10-11 0.2516998819622458 1103 12-13 0.3071560584753428 1103 14-15 0.3884027602571791 1103 16-17 0.3360154085265208 1103 18-19 0.33898797929723656 1103 20-21 0.3478565277673624 1103 22-23 0.37617097201384553 1103 24-25 0.47803665061780265 1103 26-27 0.4574044571322915 1103 28-29 0.3513090150308855 1103 30-31 0.5167810720448358 1103 32-33 0.4857366277768165 1103 34-35 0.679086446141568 1103 36-37 0.6963446322104048 1103 38-39 0.5577535005471645 1103 40-41 0.7335670475711069 1103 42-43 0.7264756032002495 1103 44-45 0.89428934938568 1103 46-47 0.9766772626573896 1103 48-49 0.8264659915596795 1103 50-51 1.2086389507501494 1103 52-53 1.1433292001284272 1103 54-55 1.0073786787798298 1103 56-57 0.9577210069581668 1103 58-59 0.856203679977078 1103 60-61 0.9126309138709345 1103 62-63 1.0163643062707877 1103 64-65 1.1514276990550982 1103 66-67 0.856397757546631 1103 68-69 0.9337093185748415 1103 70-71 1.0397372046197297 1103 72-73 1.1480625313150945 1103 74-75 1.1010436090581628 1103 76-77 1.199176776002279 1103 78-79 1.343344713291355 1103 80-81 1.4300148540539084 1103 82-83 1.3314495306477845 1103 84-85 1.3110413382687476 1103 86-87 1.1748220751829344 1103 88-89 1.3317046856199752 1103 90-91 1.4674648422570478 1103 92-93 1.4204774227251704 1103 94-95 1.4970149664309211 1103 96-97 1.3058407055599268 1103 98-99 1.2272373793318536 1103 100-101 1.1134372862023518 1104 1 0.1762798953313407 1104 2 0.16309914112382984 1104 3 0.13207088217166785 1104 4 0.19721414415543137 1104 5 0.12159248342831575 1104 6 0.08769732067568725 1104 7 0.17704833921365548 1104 8 0.23848764917108412 1104 9 0.20720871489692883 1104 10-11 0.24369421995841378 1104 12-13 0.25310498289053385 1104 14-15 0.3244186883164275 1104 16-17 0.2208602421929129 1104 18-19 0.1577891626364405 1104 20-21 0.21961899789000228 1104 22-23 0.2559072676135443 1104 24-25 0.31261405745922133 1104 26-27 0.33621749345532237 1104 28-29 0.46010422289892006 1104 30-31 0.23826533990268217 1104 32-33 0.31850425051887754 1104 34-35 0.4366414988432652 1104 36-37 0.373443883807127 1104 38-39 0.5557355545419114 1104 40-41 0.7972107135716513 1104 42-43 0.7104936667557524 1104 44-45 0.6928767871820014 1104 46-47 0.46487080914851475 1104 48-49 0.5438629391714542 1104 50-51 0.40481073085281594 1104 52-53 0.5845099924171251 1104 54-55 0.4829698585925257 1104 56-57 0.6592462214873791 1104 58-59 0.6212746019629023 1104 60-61 0.5550210043846278 1104 62-63 0.5537869190210785 1104 64-65 0.25471028986267186 1104 66-67 0.3948807715351137 1104 68-69 0.3524869918635005 1104 70-71 0.31984231413738584 1104 72-73 0.24693453868157533 1104 74-75 0.09714241004558488 1104 76-77 0.06672213080812739 1104 78-79 0.36544660976876386 1104 80-81 0.40457012226439915 1104 82-83 0.40943677644536436 1104 84-85 0.31300295811123036 1104 86-87 0.4344220124894669 1104 88-89 0.8569902347755729 1104 90-91 0.6145593918436631 1104 92-93 0.7542540184698545 1104 94-95 0.6508980431128712 1104 96-97 0.6024567276593729 1104 98-99 0.7820133481418523 1104 100-101 0.8116172164558684 1105 1 0.08566041157304483 1105 2 0.13701772218156805 1105 3 0.1785470834167313 1105 4 0.13339927629395731 1105 5 0.10658830600399227 1105 6 0.14612236888881824 1105 7 0.1663385853990107 1105 8 0.18781753377366783 1105 9 -0.06363678950349794 1105 10-11 0.10179253391226695 1105 12-13 0.09929842276389422 1105 14-15 0.28136569784597754 1105 16-17 0.22350568251857084 1105 18-19 0.13621967605131857 1105 20-21 0.21700889292043257 1105 22-23 0.26124055069214336 1105 24-25 0.3357139575779442 1105 26-27 0.22772263828316852 1105 28-29 0.21971347667901853 1105 30-31 0.21735623186130226 1105 32-33 0.369938002222824 1105 34-35 0.2856272170775611 1105 36-37 0.1655761919439982 1105 38-39 0.2846003002215838 1105 40-41 0.4574055134636055 1105 42-43 0.31366426899585775 1105 44-45 0.4884452623907194 1105 46-47 0.6180020713588448 1105 48-49 0.6166431721021226 1105 50-51 0.6019097766411576 1105 52-53 0.47273012697416306 1105 54-55 0.5024981394410801 1105 56-57 0.45635610345158284 1105 58-59 0.3747303997881275 1105 60-61 0.2984019069595547 1105 62-63 0.41942108658290067 1105 64-65 0.610549104102347 1105 66-67 0.5145145087908247 1105 68-69 0.5565612369432529 1105 70-71 0.9247643721941579 1105 72-73 0.5269943261040737 1105 74-75 0.613397066460287 1105 76-77 0.5742752594790765 1105 78-79 0.571244042380318 1105 80-81 0.6425254772198272 1105 82-83 0.5532575649420579 1105 84-85 0.5909065035823389 1105 86-87 0.8535228925754019 1105 88-89 1.1390130975361927 1105 90-91 1.2281711991921611 1105 92-93 1.283335409143831 1105 94-95 0.9035050071618933 1105 96-97 0.9797633411816626 1105 98-99 0.9684823409742727 1105 100-101 0.8272699939719033 1106 1 0.1353218402725247 1106 2 0.17086497692057634 1106 3 0.10027155611300742 1106 4 0.15623175908432785 1106 5 0.19388303243503913 1106 6 0.17993695913170882 1106 7 0.13547899259614127 1106 8 0.07995480973700353 1106 9 0.09073030170033292 1106 10-11 0.24123612840457298 1106 12-13 0.2079857354178145 1106 14-15 0.17256671819367853 1106 16-17 0.3066189736260725 1106 18-19 0.13002405674006212 1106 20-21 0.2239254100647372 1106 22-23 0.24416499543369952 1106 24-25 0.3413721319820482 1106 26-27 0.33008861129773237 1106 28-29 0.4260186359981972 1106 30-31 0.40761134917242003 1106 32-33 0.5523042639003819 1106 34-35 0.5122721023492147 1106 36-37 0.5433069058379516 1106 38-39 0.699608040947524 1106 40-41 0.6940180077093387 1106 42-43 0.7561413316828265 1106 44-45 0.9510060525372808 1106 46-47 0.7805975770280327 1106 48-49 0.7376172729548927 1106 50-51 0.8901823109654643 1106 52-53 0.7915599176023349 1106 54-55 0.8978946484420476 1106 56-57 0.7601108492990107 1106 58-59 0.760434669113728 1106 60-61 0.6193670440668768 1106 62-63 0.8869233313174618 1106 64-65 0.6729751342114199 1106 66-67 0.8185742498753186 1106 68-69 0.9435618309813023 1106 70-71 0.9548046938558876 1106 72-73 1.0819474031755725 1106 74-75 1.0901589362064286 1106 76-77 0.9647018274128705 1106 78-79 0.9950533455326678 1106 80-81 1.0390132082831585 1106 82-83 1.1596078468442563 1106 84-85 1.0498107692616792 1106 86-87 0.9367941886874185 1106 88-89 1.302826305235289 1106 90-91 1.4324614148841874 1106 92-93 1.3131303672649075 1106 94-95 1.438071260635347 1106 96-97 1.5923438059898452 1106 98-99 1.8492455355316082 1106 100-101 1.3926939411496129 1107 1 0.10820504626036609 1107 2 0.1089126924983077 1107 3 0.111966550655346 1107 4 0.08824485747821598 1107 5 0.07113236971868275 1107 6 0.1297420425560034 1107 7 0.09317429900499263 1107 8 0.04101827457404994 1107 9 0.18131193276223456 1107 10-11 0.1503270374954795 1107 12-13 0.15508537677131073 1107 14-15 0.2051957529668016 1107 16-17 0.17367807233183186 1107 18-19 0.15749170058850126 1107 20-21 0.1922319748550052 1107 22-23 0.13916733443260654 1107 24-25 0.057277324559628084 1107 26-27 0.08312930987874267 1107 28-29 0.22300132740627276 1107 30-31 0.18932037770820642 1107 32-33 0.29258182510418607 1107 34-35 0.43366614569866613 1107 36-37 0.4189189438856644 1107 38-39 0.47874417068399566 1107 40-41 0.2736687172056733 1107 42-43 0.2281201247593927 1107 44-45 0.10952000856409683 1107 46-47 0.14676005948554405 1107 48-49 0.18629963690312223 1107 50-51 0.25360036803703423 1107 52-53 0.07305843623635866 1107 54-55 0.26510968040837213 1107 56-57 0.07770791775371322 1107 58-59 -0.06349328971989365 1107 60-61 0.05230794536112171 1107 62-63 0.168398459962404 1107 64-65 0.2401199962104812 1107 66-67 0.387403191088481 1107 68-69 0.2452848935105365 1107 70-71 0.31073015775733026 1107 72-73 0.28112875355760636 1107 74-75 0.1569139650540805 1107 76-77 0.49213828460919373 1107 78-79 0.7219050530018727 1107 80-81 0.5045129743832675 1107 82-83 0.46740450987247684 1107 84-85 0.5214776958171115 1107 86-87 0.54477017496529 1107 88-89 0.8046039444057236 1107 90-91 0.9550171610248377 1107 92-93 0.681358965424895 1107 94-95 0.740441446195927 1107 96-97 0.7026977744913303 1107 98-99 0.9873244377614512 1107 100-101 1.2075699742074661 1108 1 -0.1040475003473631 1108 2 -0.040297927748248696 1108 3 0.0040343137590497236 1108 4 -0.002595591713188128 1108 5 0.019085473697160182 1108 6 0.05622127157222678 1108 7 1.8810971859295478E-4 1108 8 0.08757520601470503 1108 9 0.0015379012968779193 1108 10-11 0.019664095786033897 1108 12-13 -0.036943260553307766 1108 14-15 0.029884805985659568 1108 16-17 -0.06901570323720563 1108 18-19 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-0.6757751075052632 2316 72-73 -0.9015869666947687 2316 74-75 -0.917277917110674 2316 76-77 -0.9125160297958566 2316 78-79 -0.9045073481356063 2316 80-81 -0.9710518749902342 2316 82-83 -1.0451860215413653 2316 84-85 -0.9644721619985823 2316 86-87 -1.3069343704892553 2316 88-89 -1.4475310418201737 2316 90-91 -1.9312181762185645 2316 92-93 -1.759630104156546 2316 94-95 -1.432049222958966 2316 96-97 -1.3642771433083674 2316 98-99 -1.3127733139492008 2316 100-101 -0.6887760001993577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 41.0 6 154.0 7 308.0 8 509.0 9 903.0 10 1488.0 11 2350.0 12 3479.0 13 5100.0 14 5783.0 15 7915.0 16 10325.0 17 14877.0 18 13518.0 19 8875.0 20 8157.0 21 8950.0 22 9479.0 23 10511.0 24 11722.0 25 13501.0 26 15616.0 27 18507.0 28 22665.0 29 27260.0 30 32997.0 31 40042.0 32 49259.0 33 62063.0 34 84381.0 35 130018.0 36 186985.0 37 258786.0 38 150821.0 39 7933.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.12595061584931 16.658087630557517 13.58248272667682 17.63347902691635 2 12.220332426340127 25.823190189456568 37.43248559121321 24.523991792990092 3 27.74811596237311 29.24742959648868 29.42240800983274 13.582046431305466 4 11.38224785235217 15.634288587065633 40.18407010888815 32.799393451694044 5 10.940884391827595 43.63891441564229 32.27372369406852 13.146477498461595 6 24.44254195319654 36.98353211555757 18.098268660419457 20.47565727082643 7 23.964044547675904 38.633585082437364 19.386041227656385 18.016329142230354 8 22.029161980716463 28.129738658640296 20.84365058508407 28.99744877555917 9 24.994164665786332 23.94853806515008 22.697491377579585 28.359805891484 10-11 23.37781789513374 24.524522277918607 26.001351512371723 26.09630831457593 12-13 35.492910272963066 22.621917678532665 23.38491823186925 18.50025381663503 14-15 28.91885693578874 23.92048107026381 30.848230754414548 16.312431239532902 16-17 18.32118789934456 28.24178622349503 33.455943863039664 19.981082014120748 18-19 17.61103865684481 33.71766286295371 31.90083359585536 16.770464884346122 20-21 20.38261837567147 28.73738874098788 27.14332233733292 23.736670546007733 22-23 18.094881718183554 28.087422283957974 25.864404787748903 27.953291210109573 24-25 22.65566085471871 28.119582923907117 27.98557405092011 21.239182170454065 26-27 25.522654339728586 22.120259466376773 32.98329203678494 19.3737941571097 28-29 19.39310075785893 26.487928611418976 34.584679268812216 19.534291361909876 30-31 19.137896700710197 31.11791945443298 30.68259536078688 19.061588484069947 32-33 18.714732953886987 29.848469020496303 29.42285688169533 22.01394114392138 34-35 21.422205427587144 25.51558486817889 29.731836289306994 23.330373414926967 36-37 24.259244822968522 24.923339894967594 32.65093969595539 18.16647558610849 38-39 26.205566743999558 22.616259495724243 33.51015835899194 17.668015401284258 40-41 19.76282665310582 26.957327000221614 34.94474512395156 18.335101222721004 42-43 18.082546310333612 31.025893304941604 30.18764902698615 20.70391135773863 44-45 20.07822669827836 29.880558400698614 26.92932664645368 23.111888254569347 46-47 20.581012525993803 28.733110148438083 25.791239965657166 24.89463735991094 48-49 22.78249138574089 27.1270405306808 28.03053797700797 22.05993010657034 50-51 23.285023839176556 29.06216793683802 29.82847381934458 17.824334404640847 52-53 19.4736199939035 30.264789619793138 30.47714690398668 19.784443482316686 54-55 16.062757183173996 28.18756151584844 32.16277073094294 23.586910570034618 56-57 21.83145432851218 30.482602542736682 25.83979844837239 21.84614468037875 58-59 21.814032141041242 27.23059985130015 27.66486545601153 23.290502551647084 60-61 20.68544920117574 25.534341628515193 31.4893580555135 22.29085111479556 62-63 25.451722357905215 25.03002368841471 29.168987560011672 20.3492663936684 64-65 22.120700060842566 28.47052563324624 31.066638319716866 18.34213598619433 66-67 19.55383769119901 27.46544910854048 31.102453778440115 21.878259421820395 68-69 18.2783518364209 31.688676404217446 29.16658165284818 20.866390106513474 70-71 20.262892007958907 30.647338516307105 25.410105656275146 23.679663819458842 72-73 20.17217908518282 27.196411636717393 26.56219650141109 26.0692127766887 74-75 20.64451470422609 30.33059820745062 27.167479804846838 21.857407283476455 76-77 22.931761451177053 25.28574005293323 29.597700091657682 22.18479840423204 78-79 20.35164621715278 25.806949189337413 32.491671386622315 21.34973320688749 80-81 20.047099361616347 31.018043193322026 29.133007748420365 19.801849696641263 82-83 18.86510502785454 29.364993217954222 28.08893212567759 23.680969628513644 84-85 19.78814880347084 27.21995700623687 27.652383836042617 25.339510354249672 86-87 22.19597498129152 27.257639431262277 28.745412746600614 21.80097284084559 88-89 23.527487085195734 24.10415382693855 29.70730538725985 22.661053700605866 90-91 20.28507510177305 25.254335361900903 31.80867936442675 22.651910171899296 92-93 19.00380643339476 29.971867976175513 29.92118626223584 21.103139328193887 94-95 18.57521541768751 30.73303223552878 27.47438573925931 23.217366607524397 96-97 17.324168730117016 28.420730045197317 26.873713574048352 27.38138765063732 98-99 20.90726796997292 27.809488987949017 25.96201401459568 25.321229027482385 100-101 22.49389940340654 27.338058745276626 27.464192150429696 22.703849700887137 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5070.0 1 3358.5 2 2594.5 3 3246.5 4 2363.0 5 2187.0 6 3877.5 7 7889.0 8 10997.0 9 10404.5 10 7527.5 11 5142.5 12 4573.5 13 4336.0 14 4144.0 15 3832.0 16 3398.0 17 3170.0 18 3190.0 19 3173.0 20 3062.0 21 3121.0 22 3301.0 23 3506.0 24 3781.0 25 4287.0 26 4895.5 27 5540.0 28 6108.0 29 6928.0 30 8214.0 31 9142.0 32 10383.5 33 11777.5 34 12757.0 35 13323.0 36 14268.0 37 15728.0 38 15977.5 39 15882.0 40 16152.0 41 16620.0 42 16667.5 43 16882.0 44 20153.5 45 23731.5 46 34244.5 47 76134.0 48 232024.5 49 234803.5 50 104109.5 51 65013.5 52 24904.0 53 12313.0 54 9845.0 55 8589.5 56 7512.0 57 6649.0 58 5887.0 59 5361.5 60 4978.0 61 4576.0 62 3889.0 63 2462.5 64 1387.5 65 1169.0 66 1028.0 67 829.5 68 666.5 69 481.0 70 395.5 71 341.5 72 290.5 73 333.0 74 291.5 75 199.0 76 138.0 77 93.0 78 85.5 79 65.5 80 35.5 81 17.0 82 10.0 83 7.5 84 4.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27120021807011213 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0021219397140604624 16-17 4.0806532962701193E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.6322613185080478E-4 26-27 8.161306592540239E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002856457307389084 36-37 0.019872781552835485 38-39 0.0058353342136662715 40-41 0.016200193586192378 42-43 0.00444791209293443 44-45 7.345175933286215E-4 46-47 1.6322613185080478E-4 48-49 0.0 50-51 0.0 52-53 4.0806532962701196E-5 54-55 0.0 56-57 0.0 58-59 5.712914614778167E-4 60-61 0.0019995201151723585 62-63 9.385502581421275E-4 64-65 4.0806532962701196E-5 66-67 0.0 68-69 2.448391977762072E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22937352178334344 78-79 0.0 80-81 9.793567911048288E-4 82-83 0.0 84-85 0.0 86-87 0.102016332406753 88-89 0.3518747337373724 90-91 0.3523644121329248 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.2645226370160956E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1225294.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.874309104755604 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65333745535895 14.041862678704106 2 13.057287737442552 5.190091451273364 3 5.542881413190685 3.304828156102687 4 2.9571102675343877 2.3508209405529983 5 1.7453670894946383 1.7343982518942036 6 1.2067800841900969 1.4390352248794187 7 0.7925229029293016 1.1025591601790614 8 0.6229075746368131 0.9903886145620516 9 0.46626193726252835 0.8339970478443889 >10 2.566521656147229 9.319944201981398 >50 0.19821199490246624 2.695006012579215 >100 0.13035934084178136 4.742311559987581 >500 0.014804215363735425 2.0470603643775775 >1k 0.032075799954760086 13.821151364230932 >5k 0.006990879477319506 10.037681669332093 >10k+ 0.006579651272771299 26.348863301518932 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 53595 4.374052268271941 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 44110 3.5999523379694995 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28936 2.3615556756174434 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28664 2.339356921685734 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 19759 1.612592569620026 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 18419 1.5032310612799866 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 16794 1.3706098291512079 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15701 1.2814067480947429 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 14745 1.2033846570700584 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13959 1.1392367872526918 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 12769 1.0421172388014632 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12300 1.0038407108824494 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11158 0.9106385895956399 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 10834 0.8841959562358096 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10390 0.8479597549649309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10261 0.8374316694605539 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9584 0.7821796238290565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9446 0.7709170207313509 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8884 0.7250504776812748 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 8492 0.6930581558385172 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8273 0.675184894400854 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 8101 0.6611474470616847 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7471 0.6097312155286813 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6928 0.5654153207311878 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6892 0.5624772503578732 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6795 0.5545607829631092 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6722 0.5486030291505549 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6609 0.5393807527009844 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6266 0.5113874710885714 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6132 0.5004513202545675 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5846 0.47710998339990235 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5249 0.42838698304243716 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5127 0.4184301889995381 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4662 0.38048011334422593 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4473 0.3650552438843249 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4408 0.35975039459917374 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4369 0.35656748502808305 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4189 0.34187713316151064 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4097 0.3343687310963736 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3824 0.31208836409873875 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3818 0.31159868570318633 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3742 0.3053960926928557 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3548 0.2895631579033277 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3428 0.2797695899922794 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3424 0.2794431377285778 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3381 0.2759337758937855 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3302 0.2694863436856787 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3255 0.26565052958718477 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3214 0.2623043938842433 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3134 0.2557753486102111 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3124 0.25495921795095705 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 3044 0.24843017267692488 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2965 0.24198274046881807 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2933 0.2393711223592052 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2714 0.2214978609215421 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2668 0.2177436598889736 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2636 0.21513204177936068 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2533 0.20672589598904426 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2484 0.20272685575869953 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2451 0.20003362458316126 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2435 0.19872781552835483 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2380 0.1942390969024577 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2311 0.18860779535360492 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2274 0.18558811191436506 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.1800384234314377 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2054 0.16763323741077651 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1993 0.16265484038932698 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1972 0.16094096600489352 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1901 0.15514643832418995 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1883 0.1536774031375327 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1860 0.15180030262124844 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1848 0.15082094583014363 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1751 0.1429044784353796 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1730 0.14119060405094613 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1726 0.1408641517872445 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1721 0.1404560864576175 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1711 0.1396399557983635 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.13531446330431715 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1622 0.13237639293100267 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1614 0.13172348840359946 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1611 0.13147864920582325 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1570 0.12813251350288177 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1563 0.12756122204140394 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1549 0.1264186391184483 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1481 0.12086895063552094 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1465 0.1195631415807145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1460 0.1191550762510875 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1358 0.11083054352669645 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1318 0.10756602088968034 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1299 0.10601537263709772 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1290 0.10528085504376908 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 1279 0.10438311131858967 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1252 0.10217955853860379 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.161306592540239E-5 0.0 10-11 0.0 0.0 0.0 2.04032664813506E-4 0.0 12-13 0.0 0.0 0.0 2.448391977762072E-4 0.0 14-15 0.0 0.0 0.0 2.8564573073890836E-4 0.0 16-17 0.0 0.0 0.0 5.304849285151156E-4 0.0 18-19 0.0 0.0 0.0 6.120979944405179E-4 0.0 20-21 0.0 0.0 0.0 8.977437251794263E-4 0.0 22-23 0.0 0.0 0.0 0.002244359312948566 0.0 24-25 0.0 0.0 0.0 0.003999040230344717 0.0 26-27 0.0 0.0 0.0 0.0077940477958759295 0.0 28-29 0.0 0.0 0.0 0.01607777398730427 0.0 30-31 0.0 0.0 0.0 0.03790926912234941 0.0 32-33 0.0 0.0 0.0 0.07675708850284095 0.0 34-35 0.0 0.0 0.0 0.14943352370941176 0.0 36-37 0.0 0.0 0.0 0.24732839628693196 0.0 38-39 0.0 0.0 0.0 0.35921990967065864 0.0 40-41 0.0 0.0 0.0 0.5002880941227167 0.0 42-43 0.0 0.0 0.0 0.6313586779989129 0.0 44-45 0.0 0.0 0.0 0.7690807267480295 0.0 46-47 0.0 0.0 0.0 0.9616059492660537 0.0 48-49 0.0 0.0 0.0 1.177758154369482 0.0 50-51 0.0 0.0 0.0 1.3850145352870413 0.0 52-53 0.0 0.0 0.0 1.5738263633054597 0.0 54-55 0.0 0.0 0.0 1.7712075632460453 0.0 56-57 0.0 0.0 0.0 2.0088240046878543 0.0 58-59 0.0 0.0 0.0 2.3493545222615957 0.0 60-61 0.0 0.0 0.0 2.8291577368370366 0.0 62-63 0.0 0.0 0.0 3.2132288250819805 0.0 64-65 0.0 0.0 0.0 3.505036342298257 0.0 66-67 0.0 0.0 0.0 3.846301377465327 0.0 68-69 0.0 0.0 0.0 4.247960081417194 0.0 70-71 0.0 0.0 0.0 4.742820906655872 0.0 72-73 0.0 0.0 0.0 5.272204058780995 0.0 74-75 0.0 0.0 0.0 5.786774439440657 0.0 76-77 0.0 0.0 0.0 6.226872897443389 0.0 78-79 0.0 0.0 0.0 6.781148034675759 0.0 80-81 0.0 0.0 0.0 7.399489428659571 0.0 82-83 0.0 0.0 0.0 8.029215845339976 0.0 84-85 0.0 0.0 0.0 8.982578874947563 0.0 86-87 0.0 0.0 0.0 9.978503118435249 0.0 88-89 0.0 0.0 0.0 10.704247307176889 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGC 40 5.413977E-4 47.454254 4 CTTAACG 30 8.3387145E-5 39.54683 20-21 CGTCACT 85 3.5895027E-7 39.18872 1 GCTTATG 445 0.0 38.496784 1 GTAAGCG 205 0.0 38.19489 94-95 ATCACGC 50 0.0016015107 38.069042 1 TATGTAC 525 0.0 33.44395 4 GGGGTGC 100 1.3091048E-6 33.217976 7 CTTATGT 505 0.0 31.9494 2 AGTACTC 605 0.0 30.590347 5 TACCTGG 710 0.0 28.071533 2 TAGACTA 85 6.443623E-4 27.914268 5 ATACTGG 85 6.443623E-4 27.914268 6 AAGTACT 615 0.0 27.7781 4 TTATGTA 595 0.0 27.116716 3 CGATCAG 35 0.008432931 27.116716 18-19 CTCCGAC 130 0.0 25.55229 94-95 ACTGTCC 130 1.0096968E-5 25.55229 8 CTGGTCG 135 1.352885E-5 24.60591 9 AGTATCG 100 0.0016630082 23.727127 5 >>END_MODULE