FastQCFastQC Report
Fri 27 May 2016
SRR939313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683846
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.5951256825149094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.4054408776099477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57170.3395203599379041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25970.1542302562110787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.10862038452447553No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG17270.10256282344109854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90850.034.316711
GGTATCA60050.029.396471
GTACATG87650.027.8276421
TACATGG86700.027.3459452
TATCAAC113650.027.3283772
ATCAACG113950.027.0899773
TCAACGC116350.026.5719364
CAACGCA117200.026.3387625
AACGCAG119050.025.871936
ACATGGG89550.025.5754643
CATGGGG41750.024.1916284
ACGCAGA134700.022.7604877
AGTACTT69000.022.66091212-13
CGCAGAG136100.022.5270298
ATGGGGA27150.021.6567765
GCAGAGT142300.021.310529
GAGTACT67750.021.1557612-13
TACTTTT75750.020.45944814-15
ATGGGAG19450.019.991085
GTACTTT71650.019.7449914-15
Produced by FastQC (version 0.11.5)