##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939313_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683846 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.258442874229594 33.0 31.0 34.0 31.0 34.0 2 32.33312606972372 34.0 31.0 34.0 31.0 34.0 3 32.389773174031355 34.0 31.0 34.0 31.0 34.0 4 35.88289724832318 37.0 35.0 37.0 35.0 37.0 5 35.90980588486121 37.0 35.0 37.0 35.0 37.0 6 35.99968465049654 37.0 37.0 37.0 35.0 37.0 7 35.947839648043825 37.0 37.0 37.0 35.0 37.0 8 35.93772649042727 37.0 37.0 37.0 35.0 37.0 9 37.63023518777845 39.0 38.0 39.0 35.0 39.0 10-11 37.54062099503162 39.0 38.0 39.0 35.0 39.0 12-13 37.449177953328274 39.0 37.0 39.0 35.0 39.0 14-15 38.75629303392353 40.0 38.0 41.0 35.0 41.0 16-17 38.687085992424485 40.0 38.0 41.0 35.0 41.0 18-19 38.61406268744291 40.0 38.0 41.0 35.0 41.0 20-21 38.58392186696408 40.0 38.0 41.0 34.5 41.0 22-23 38.59030012245775 40.0 38.0 41.0 34.5 41.0 24-25 38.50788759779695 40.0 38.0 41.0 34.0 41.0 26-27 38.416706753467956 40.0 38.0 41.0 34.0 41.0 28-29 38.26474689490607 40.0 38.0 41.0 34.0 41.0 30-31 38.07040014348105 40.0 38.0 41.0 34.0 41.0 32-33 37.84160546748337 40.0 37.5 41.0 33.5 41.0 34-35 37.55516270490294 40.0 37.0 41.0 33.0 41.0 36-37 37.31940242753791 40.0 37.0 41.0 32.5 41.0 38-39 36.72635027193698 39.5 35.5 40.5 31.0 41.0 40-41 36.67780604639617 39.0 36.0 40.5 31.0 41.0 42-43 36.84856572394388 40.0 36.0 41.0 31.0 41.0 44-45 36.72993581360765 40.0 36.0 41.0 31.0 41.0 46-47 36.42057973235082 40.0 35.5 41.0 30.0 41.0 48-49 36.145328016932666 39.0 35.0 41.0 30.0 41.0 50-51 34.982366558462 38.0 34.0 40.0 27.5 40.5 52-53 35.07142428701912 38.5 34.5 39.5 28.0 40.5 54-55 35.5293580885663 39.0 35.0 40.5 28.0 41.0 56-57 35.41334421318814 39.0 35.0 40.5 28.0 41.0 58-59 35.178865228767954 38.0 35.0 40.0 28.0 41.0 60-61 34.86551175107462 38.0 35.0 40.0 27.0 41.0 62-63 34.46287101076939 37.0 34.0 40.0 26.0 41.0 64-65 34.04921174501706 36.5 34.0 39.5 26.0 41.0 66-67 33.67821196237661 36.0 34.0 39.0 25.5 41.0 68-69 33.27629575388723 35.5 33.0 39.0 24.5 40.5 70-71 32.85642333087468 35.0 33.0 38.0 23.5 40.0 72-73 32.428479207718524 35.0 33.0 37.0 22.5 39.5 74-75 31.98686310980933 35.0 32.0 37.0 21.0 39.0 76-77 31.546544933444032 35.0 32.0 36.0 20.0 39.0 78-79 31.177051523714162 35.0 31.5 36.0 18.5 38.0 80-81 30.80869568832304 35.0 31.0 35.0 17.0 37.0 82-83 30.414801591119378 34.0 31.0 35.0 11.5 37.0 84-85 30.033149112210975 34.0 31.0 35.0 7.0 36.0 86-87 29.62320069650075 34.0 30.0 35.0 2.0 36.0 88-89 29.21995033987669 34.0 29.5 35.0 2.0 35.5 90-91 28.833170313674763 34.0 29.0 35.0 2.0 35.0 92-93 28.404210658219338 34.0 29.0 35.0 2.0 35.0 94-95 27.940855339502544 34.0 28.5 35.0 2.0 35.0 96-97 27.355308026981092 33.5 27.0 35.0 2.0 35.0 98-99 26.575427622240987 33.0 25.5 35.0 2.0 35.0 100-101 23.841049894111457 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25630732992281224 1101 2 0.33129608936989996 1101 3 0.31711612889618834 1101 4 0.13999381674853595 1101 5 0.11163629919101226 1101 6 0.2233347021137817 1101 7 0.21391818478512192 1101 8 0.04594439793693539 1101 9 0.24340473397536755 1101 10-11 0.3173741006652193 1101 12-13 0.36537279317902005 1101 14-15 0.4806991412569346 1101 16-17 0.46716835917019495 1101 18-19 0.45455532706588997 1101 20-21 0.5283416582103442 1101 22-23 0.5366156117947796 1101 24-25 0.49263013163349 1101 26-27 0.5239603117352587 1101 28-29 0.5357180554329446 1101 30-31 0.43668111783580343 1101 32-33 0.4816309500507643 1101 34-35 0.4115868109507872 1101 36-37 0.3650721614628125 1101 38-39 0.6344954992914111 1101 40-41 0.5292985853003032 1101 42-43 0.4426879478956991 1101 44-45 0.5842076376695431 1101 46-47 0.5479488620852422 1101 48-49 0.5771966403712625 1101 50-51 0.6476583891084715 1101 52-53 0.7029290422545813 1101 54-55 0.6781805754320587 1101 56-57 0.5450199845558217 1101 58-59 0.619659873066496 1101 60-61 0.5213985239757974 1101 62-63 0.6407551103561175 1101 64-65 0.4531011322465659 1101 66-67 0.6649677694522111 1101 68-69 0.7539255263659399 1101 70-71 0.6122402161657661 1101 72-73 0.7526073062353618 1101 74-75 0.6513480930231239 1101 76-77 0.6070798794198282 1101 78-79 0.619490157740163 1101 80-81 0.6451230098335721 1101 82-83 0.6318587615900171 1101 84-85 0.7158563519086307 1101 86-87 0.6837057812427041 1101 88-89 0.5539549536673327 1101 90-91 0.6097478716976887 1101 92-93 0.42431013520278427 1101 94-95 0.6616490464983507 1101 96-97 0.6963738121257563 1101 98-99 0.6473863038787151 1101 100-101 0.3942466727169247 1102 1 0.2795319892254611 1102 2 0.15570167043293281 1102 3 0.2555084677220876 1102 4 0.16225555187943286 1102 5 0.16511106708649947 1102 6 0.1537565294775547 1102 7 0.23291563945014815 1102 8 0.33575737467980105 1102 9 0.40716679589966986 1102 10-11 0.4053533807454386 1102 12-13 0.3199401877221746 1102 14-15 0.458239300309927 1102 16-17 0.5167350026974518 1102 18-19 0.4901745904207786 1102 20-21 0.4741205428908444 1102 22-23 0.5043550133365926 1102 24-25 0.40854151987623055 1102 26-27 0.43481188257636205 1102 28-29 0.43901805989845144 1102 30-31 0.4201035581909309 1102 32-33 0.30243571433326366 1102 34-35 0.21159411813253115 1102 36-37 0.2980563738907023 1102 38-39 0.6508130450526366 1102 40-41 0.5625821892023382 1102 42-43 0.4224159989485301 1102 44-45 0.4979938538890565 1102 46-47 0.48839248920787526 1102 48-49 0.3722490694002616 1102 50-51 0.4590078631943655 1102 52-53 0.5151517245586135 1102 54-55 0.46719199493330166 1102 56-57 0.43036725836810774 1102 58-59 0.5114807009701892 1102 60-61 0.42780265984066546 1102 62-63 0.4333304047746509 1102 64-65 0.2575252248366766 1102 66-67 0.34497247852488755 1102 68-69 0.2564262063397962 1102 70-71 0.33675276625040595 1102 72-73 0.3550117749829482 1102 74-75 0.20059565628099563 1102 76-77 0.25536654827864425 1102 78-79 0.15621199362897897 1102 80-81 0.14194479173043462 1102 82-83 0.14422088927895516 1102 84-85 0.19369610541303217 1102 86-87 -0.045818480630451575 1102 88-89 0.0362370677162005 1102 90-91 0.1384180052161348 1102 92-93 0.08140276996954299 1102 94-95 -0.018375757324115227 1102 96-97 0.0230706006193806 1102 98-99 0.042441980544044355 1102 100-101 -0.13745147572441496 1103 1 0.3888880780795745 1103 2 0.35503212672513484 1103 3 0.3371699793504632 1103 4 0.2585443318265419 1103 5 0.2915900333077559 1103 6 0.3547655314376925 1103 7 0.2785656429140104 1103 8 0.34470432057431566 1103 9 0.26346959429081096 1103 10-11 0.4141336538605884 1103 12-13 0.38199156694961545 1103 14-15 0.5392862824730855 1103 16-17 0.5276816144313798 1103 18-19 0.4641308479852384 1103 20-21 0.5105955969881819 1103 22-23 0.6190819185613137 1103 24-25 0.525972884514708 1103 26-27 0.5238184068580196 1103 28-29 0.5209477150202773 1103 30-31 0.4419022386641345 1103 32-33 0.4912401122125658 1103 34-35 0.5359829506488012 1103 36-37 0.49865419532669364 1103 38-39 0.8374953194636774 1103 40-41 0.760564389038521 1103 42-43 0.7491254137192485 1103 44-45 0.5852529628213361 1103 46-47 0.5396449801305252 1103 48-49 0.42723254475184547 1103 50-51 0.3949955318537661 1103 52-53 0.5089829979142024 1103 54-55 0.5572672218161117 1103 56-57 0.6035796011190655 1103 58-59 0.6225695347089939 1103 60-61 0.7108886328388593 1103 62-63 0.6148054792476358 1103 64-65 0.5384018666043033 1103 66-67 0.5055663878369714 1103 68-69 0.4781376790506897 1103 70-71 0.34902318366588503 1103 72-73 0.39006725786537544 1103 74-75 0.3247888826014389 1103 76-77 0.3512510754210467 1103 78-79 0.1448134500801146 1103 80-81 0.10509665956584868 1103 82-83 0.0627920675210305 1103 84-85 0.20002111325765526 1103 86-87 0.049424621194798846 1103 88-89 0.04525532836960622 1103 90-91 0.04252288273812965 1103 92-93 0.11604870237945164 1103 94-95 0.22776142782122477 1103 96-97 -0.016987510721296673 1103 98-99 0.23188759998403796 1103 100-101 -0.2483658939386082 1104 1 0.10949273211503652 1104 2 0.13939499199438643 1104 3 0.22303858060598714 1104 4 0.11567044750912459 1104 5 0.1357100139238625 1104 6 0.13535613848303996 1104 7 0.18967448412442423 1104 8 0.3029366961151325 1104 9 0.32632710365019335 1104 10-11 0.23847006422906958 1104 12-13 0.3100064833644822 1104 14-15 0.28403350791539594 1104 16-17 0.26297443953922794 1104 18-19 0.28273938830184164 1104 20-21 0.27185892341679363 1104 22-23 0.3515849433359648 1104 24-25 0.3986334351187111 1104 26-27 0.3134017797826445 1104 28-29 0.2993364524064859 1104 30-31 0.37742485162600303 1104 32-33 0.3044549329748705 1104 34-35 0.2507199773745086 1104 36-37 0.22670894058049385 1104 38-39 0.7703690770889651 1104 40-41 0.5692101768418709 1104 42-43 0.3332404770969859 1104 44-45 0.37801017160363415 1104 46-47 0.4352231475921755 1104 48-49 0.40356962183767564 1104 50-51 0.34875844653295474 1104 52-53 0.2728001605805588 1104 54-55 0.3196735488211786 1104 56-57 0.3635690777538656 1104 58-59 0.21647849374308947 1104 60-61 0.19191905953795185 1104 62-63 0.2967767533904109 1104 64-65 0.15573342941509338 1104 66-67 0.18572735019040465 1104 68-69 0.130178728885042 1104 70-71 0.11457998766682209 1104 72-73 -0.0129089382667118 1104 74-75 0.02911894252681435 1104 76-77 -0.06459506173585794 1104 78-79 -0.05148492255945314 1104 80-81 -0.18719178832884253 1104 82-83 -0.20650670736606003 1104 84-85 -0.22950539865457031 1104 86-87 -0.20249179525490746 1104 88-89 -0.4190665836680445 1104 90-91 -0.4177464647340443 1104 92-93 -0.2852409047482496 1104 94-95 -0.031748400320900316 1104 96-97 0.053710459987481585 1104 98-99 0.27619718329362186 1104 100-101 0.08793084753976288 1105 1 0.14671474277764673 1105 2 0.18405835833102202 1105 3 0.27185731632571475 1105 4 0.23737951072313734 1105 5 0.15625266434274465 1105 6 0.1880652016970572 1105 7 0.13407585503074415 1105 8 0.15447173038779027 1105 9 0.2206111859045734 1105 10-11 0.3082284419899253 1105 12-13 0.36725914901565915 1105 14-15 0.4461334698346704 1105 16-17 0.42372177388805454 1105 18-19 0.36633725578089127 1105 20-21 0.3371906065851107 1105 22-23 0.373322186291027 1105 24-25 0.37258659581939213 1105 26-27 0.40338807071942284 1105 28-29 0.4194942970371116 1105 30-31 0.5160663215422261 1105 32-33 0.558957027415083 1105 34-35 0.5547513939777033 1105 36-37 0.503516442484532 1105 38-39 0.8595038828772346 1105 40-41 0.6947105576491808 1105 42-43 0.385266562397824 1105 44-45 0.3571824869120874 1105 46-47 0.5850588292372692 1105 48-49 0.7187409851278446 1105 50-51 0.5912557808817738 1105 52-53 0.4783864134366098 1105 54-55 0.622416884693223 1105 56-57 0.6193167929099985 1105 58-59 0.5912827587849847 1105 60-61 0.7063533702767231 1105 62-63 0.6088904243728877 1105 64-65 0.6198495756451052 1105 66-67 0.6698994750951996 1105 68-69 0.5535315468591477 1105 70-71 0.4941746182837221 1105 72-73 0.4931617014895622 1105 74-75 0.3487916386425809 1105 76-77 0.388226910846015 1105 78-79 0.4810801954908115 1105 80-81 0.5759359725241637 1105 82-83 0.48993769476646065 1105 84-85 0.5581155076668303 1105 86-87 0.5444607942348121 1105 88-89 0.5528740103913599 1105 90-91 0.6680113418158413 1105 92-93 0.5649774882448995 1105 94-95 0.7537839683005778 1105 96-97 0.6232548240392717 1105 98-99 0.7223371299805983 1105 100-101 0.31215361981698564 1106 1 0.07754722347711152 1106 2 0.139594029431386 1106 3 0.11727188241258801 1106 4 0.021994151221697678 1106 5 0.09120569196808503 1106 6 0.020670168676872436 1106 7 0.14209855059394982 1106 8 0.17565524820008704 1106 9 0.1243280494521457 1106 10-11 0.270115894319936 1106 12-13 0.31883035169447993 1106 14-15 0.25774522688053025 1106 16-17 0.22996677435312307 1106 18-19 0.2634372464794339 1106 20-21 0.29689798264315215 1106 22-23 0.39714955118731865 1106 24-25 0.2637152350157521 1106 26-27 0.4161260485819511 1106 28-29 0.36757149915784026 1106 30-31 0.2587637450377187 1106 32-33 0.2400322762291509 1106 34-35 0.1957313958380027 1106 36-37 0.20681529840491208 1106 38-39 0.7488101799658793 1106 40-41 0.40880096784358244 1106 42-43 0.22092761718342757 1106 44-45 0.217382096250752 1106 46-47 0.21584610483953526 1106 48-49 0.1671281119067487 1106 50-51 -0.002530657589062457 1106 52-53 0.038247635648502865 1106 54-55 0.08149008718601891 1106 56-57 0.17838980937358428 1106 58-59 0.1674165137845236 1106 60-61 0.2518289539433596 1106 62-63 0.08553929424545714 1106 64-65 0.2547306099674884 1106 66-67 0.17955569312143638 1106 68-69 0.2211055399760653 1106 70-71 0.169495239621277 1106 72-73 0.2395337599027343 1106 74-75 0.291666906059568 1106 76-77 0.3488307616505928 1106 78-79 0.5557229263995644 1106 80-81 0.5141476737612578 1106 82-83 0.5131924617648806 1106 84-85 0.5587917238327691 1106 86-87 0.5280148822266248 1106 88-89 0.3562815073683865 1106 90-91 0.35039437595220235 1106 92-93 0.3590212051084478 1106 94-95 0.2524531153566656 1106 96-97 0.361540936958999 1106 98-99 0.42046976880488884 1106 100-101 0.12059618329945465 1107 1 0.11784249408238878 1107 2 0.11826484800653958 1107 3 0.1818786460284585 1107 4 0.1617144324385933 1107 5 0.14371806030371914 1107 6 0.13191634760129745 1107 7 0.08180991448161734 1107 8 0.13786360694781052 1107 9 0.12742307607928893 1107 10-11 0.241893974097934 1107 12-13 0.30166895160245843 1107 14-15 0.39279015238516024 1107 16-17 0.3283916173189496 1107 18-19 0.36971914822473195 1107 20-21 0.2657923764966128 1107 22-23 0.18334623893455415 1107 24-25 0.2844241270197543 1107 26-27 0.37243095703664864 1107 28-29 0.3435687240849816 1107 30-31 0.3175820919075534 1107 32-33 0.39053860218250236 1107 34-35 0.3950354874653428 1107 36-37 0.27299953756054407 1107 38-39 0.726493671849326 1107 40-41 0.5789519917313228 1107 42-43 0.4139581893549362 1107 44-45 0.5607204946270841 1107 46-47 0.6795466894596416 1107 48-49 0.6543479740091698 1107 50-51 0.5117604325722667 1107 52-53 0.5005048613603691 1107 54-55 0.5576726142144395 1107 56-57 0.4039566839923623 1107 58-59 0.5119880004459745 1107 60-61 0.5283992064464513 1107 62-63 0.5397195598391917 1107 64-65 0.5210503925273358 1107 66-67 0.49065009910246005 1107 68-69 0.7171612269136105 1107 70-71 0.6491562384334841 1107 72-73 0.5668562782991913 1107 74-75 0.6098454167229121 1107 76-77 0.7006308137277983 1107 78-79 0.8969612937435869 1107 80-81 0.767119997938277 1107 82-83 0.6088358113990928 1107 84-85 0.6222044570655143 1107 86-87 0.943299006170367 1107 88-89 0.8666675207158434 1107 90-91 0.7976687051015219 1107 92-93 0.8650564535274015 1107 94-95 0.8415861012708525 1107 96-97 0.8781783547858133 1107 98-99 1.0603148926309238 1107 100-101 0.8886255962181657 1108 1 0.057377012142111994 1108 2 0.05883471986057032 1108 3 0.043261897839215635 1108 4 0.11525169184444906 1108 5 0.14118180241209188 1108 6 0.13102660645683528 1108 7 0.14594750134503442 1108 8 0.11778717739732514 1108 9 0.24729270832190053 1108 10-11 0.23018565742802366 1108 12-13 0.2618485063558893 1108 14-15 0.3175321684709118 1108 16-17 0.25116717900436925 1108 18-19 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-0.7281907135372663 2316 74-75 -0.6720964551757476 2316 76-77 -0.6696717176572626 2316 78-79 -0.6864006234318651 2316 80-81 -0.6821471123496217 2316 82-83 -0.6554663998469366 2316 84-85 -0.5566091991508983 2316 86-87 -0.3394111994046263 2316 88-89 -0.5840562221667795 2316 90-91 -0.5016567162243106 2316 92-93 -0.6436124541124855 2316 94-95 -0.5271637558099371 2316 96-97 -0.5715803975866471 2316 98-99 -0.6201108922813816 2316 100-101 5.050410056206545E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5126.0 3 955.0 4 930.0 5 1127.0 6 1290.0 7 1465.0 8 1849.0 9 2343.0 10 2670.0 11 3559.0 12 5050.0 13 7184.0 14 7403.0 15 7885.0 16 10817.0 17 14385.0 18 12292.0 19 8107.0 20 8222.0 21 8787.0 22 9103.0 23 10114.0 24 11567.0 25 13244.0 26 15531.0 27 18429.0 28 22388.0 29 27380.0 30 33547.0 31 42808.0 32 56065.0 33 74822.0 34 106530.0 35 167018.0 36 256174.0 37 372732.0 38 296928.0 39 38018.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.468908229638515 18.0748794313232 13.947375003424401 23.508837335613887 2 15.814225211595815 19.98159338027993 39.35197276248619 24.852208645638065 3 19.260036610881773 24.205480209956328 29.06608936410456 27.46839381505734 4 13.072122547876214 15.765591008839785 35.357351968125364 35.80493447515863 5 14.66255887054253 36.802405643478686 32.724285311316116 15.810750174662665 6 33.43532952180295 34.382722911807875 16.917832528480954 15.26411503790822 7 29.911544309005688 32.27262214014374 19.68851134369766 18.12732220715292 8 27.402518475879674 33.771434280100756 19.374757678758744 19.451289565260826 9 27.584912953629782 16.310899008370132 19.4556888631194 36.6484991748807 10-11 26.003235230897438 25.705071927548907 26.28147935853119 22.01021348302247 12-13 26.66628219355387 23.125284824717422 27.257885241315837 22.950547740412873 14-15 23.48066938968705 24.440941398373443 25.563072370936506 26.515316841003 16-17 23.205123746491424 27.79368520300322 26.001194320172328 22.999996730333027 18-19 22.973197574416908 25.798772936819773 28.798432498888744 22.42959698987457 20-21 23.59423782789023 25.214647989193693 28.600509980425176 22.590604202490898 22-23 23.120829770623445 25.07275363396409 28.812699356853415 22.993717238559057 24-25 23.04934010707977 25.337680858494423 28.865101978042638 22.74787705638317 26-27 22.824288216007346 25.656910122251254 28.651476865533144 22.867324796208262 28-29 22.603333130099738 25.79293074245198 28.994633146011438 22.60910298143684 30-31 22.663560781990626 25.55375241364769 28.78929677168209 22.993390032679592 32-33 22.499942748085928 25.660159181140003 28.670836588761432 23.169061482012637 34-35 23.140871859592924 25.74642207214132 28.21968816700691 22.893017901258844 36-37 23.280122153944635 25.500351827939284 28.29036677503385 22.929159243082236 38-39 22.93026963053612 25.61458783410847 28.44018751254253 23.01495502281287 40-41 22.810003303585166 25.601860741362675 28.654039497521115 22.93409645753104 42-43 22.926330430799347 25.71128385684915 28.0362237195817 23.32616199276981 44-45 22.78491054466504 26.051290310074048 27.949580533660196 23.214218611600714 46-47 22.884313869934562 26.245271691168025 27.534616603980446 23.335797834916967 48-49 23.237839964516233 26.102491887443442 27.415653721873017 23.244014426167308 50-51 23.124014422869 26.397435404152613 27.26544420056366 23.213105972414727 52-53 23.356763085091313 26.29715964594379 27.187520040565833 23.158557228399065 54-55 22.74066867952078 26.333297148085542 27.24629866667303 23.67973550572065 56-57 22.991980443013446 26.680350594758966 27.13907521688579 23.1885937453418 58-59 23.0044660183756 26.071179664383877 27.34910122199945 23.575253095241074 60-61 23.027701904846754 26.11832164673345 27.74341107283613 23.110565375583672 62-63 23.01680996607464 26.28753220582858 27.503166148370813 23.192491679725972 64-65 22.75654084377317 26.091105025140916 27.532449946341785 23.619904184744126 66-67 22.78689629745412 26.55605125408812 27.284962754996723 23.372089693461035 68-69 22.78766764478101 26.283879490266788 27.57557514436037 23.352877720591835 70-71 22.64452568941221 26.48200804468367 27.424382717890772 23.44908354801335 72-73 22.897512947123953 26.382483187083476 27.189556871595432 23.530446994197135 74-75 22.734323403793276 26.70958973903114 26.909896747181232 23.64619010999435 76-77 22.811767754834364 26.4736608701902 26.99281775320902 23.721753621766418 78-79 22.814093547668556 26.564045464480614 27.199310106892284 23.42255088095855 80-81 22.684074461709113 27.027018162192462 27.054480613775354 23.234426762323068 82-83 22.787425751491234 26.690903733852313 26.856026333729567 23.665644180926893 84-85 22.80338708484532 26.510642942519517 26.71092875837109 23.975041214264074 86-87 22.84644161247878 26.377690456848313 26.946171180037386 23.829696750635524 88-89 22.79889159068547 26.256511874898038 26.989605754757136 23.954990779659358 90-91 22.61258369611843 26.575527413070287 27.129414796859237 23.682474093952045 92-93 22.715245182649724 26.123561149040285 27.139743579799735 24.021450088510253 94-95 22.642349205629472 26.018625302561233 27.176874746063344 24.162150745745944 96-97 22.849944306262458 25.898065246690145 27.117868472094365 24.134121974953032 98-99 22.66196632302016 25.927975327371993 26.90677277241028 24.503285577197566 100-101 22.831755526301194 25.45155542883525 27.47331765368334 24.243371391180226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3853.0 1 2401.0 2 1256.5 3 1564.5 4 1260.5 5 1180.0 6 2034.5 7 4034.0 8 6003.0 9 6071.5 10 4479.5 11 3295.5 12 3125.5 13 3129.5 14 3267.0 15 3319.0 16 3238.5 17 3215.0 18 3260.0 19 3317.0 20 3464.5 21 3835.5 22 4330.0 23 5091.5 24 5885.5 25 6588.0 26 7740.0 27 9218.0 28 10339.0 29 11814.5 30 13848.5 31 15824.5 32 18259.0 33 19706.5 34 20941.0 35 22837.5 36 25431.5 37 28980.0 38 32333.0 39 33842.0 40 35359.5 41 40173.5 42 44503.5 43 48010.5 44 52075.0 45 55745.0 46 57085.0 47 58842.5 48 68159.0 49 71528.0 50 67055.0 51 65537.5 52 65166.5 53 66921.5 54 68450.5 55 65391.0 56 57904.5 57 51806.5 58 47731.0 59 42292.5 60 36575.0 61 31277.5 62 26504.0 63 21406.0 64 16723.0 65 12943.0 66 9992.0 67 8716.5 68 6923.0 69 4780.5 70 3633.5 71 2874.5 72 2430.5 73 2052.0 74 1550.0 75 1239.0 76 913.0 77 554.5 78 371.0 79 286.5 80 188.0 81 131.0 82 103.0 83 76.5 84 52.5 85 31.0 86 17.5 87 13.0 88 11.0 89 8.5 90 6.0 91 6.0 92 6.5 93 4.0 94 3.5 95 5.0 96 4.5 97 3.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.280310669740582 2 0.1739470236589332 3 0.1737094722438988 4 0.19372317896054628 5 0.20571952541978303 6 0.10434445905385646 7 0.11800366541833399 8 0.13029695114636375 9 0.1348698158857758 10-11 0.12171540627824635 12-13 0.11227273753062927 14-15 0.12679306777460647 16-17 0.10199863883039186 18-19 0.12997031795069144 20-21 0.1547053590411475 22-23 0.15788260921723246 24-25 0.18071723898741332 26-27 0.16141618651586903 28-29 0.15996118409878338 30-31 0.14974647325230456 32-33 0.159129754146163 34-35 0.33494749519849204 36-37 0.45137738249222314 38-39 0.41877345077875294 40-41 0.4085884338591534 42-43 0.43145275755621354 44-45 0.4341845988291091 46-47 0.43795572754278006 48-49 0.4505459525396028 50-51 0.4442508400411914 52-53 0.4488830926343621 54-55 0.43281867819266134 56-57 0.39766106876757135 58-59 0.38679309152974795 60-61 0.3435290400666094 62-63 0.44653727241089747 64-65 0.44511196392069113 66-67 0.4541983055457566 68-69 0.46512567063733856 70-71 0.45776157677127244 72-73 0.45164462783413684 74-75 0.45544545047468715 76-77 0.4324326571432304 78-79 0.41954549287761467 80-81 0.4163682427015297 82-83 0.4283645891607664 84-85 0.4300868369197658 86-87 0.4318684725325238 88-89 0.438846545349159 90-91 0.4568707589648935 92-93 0.46138423585054694 94-95 0.459602600237789 96-97 0.4581772917475826 98-99 0.45470310230270466 100-101 0.4372727672245562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1683846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.43228674294285 #Duplication Level Percentage of deduplicated Percentage of total 1 68.21247365095385 28.261987677517535 2 15.100501108392198 12.512965837700637 3 6.18762060632915 7.691018136539136 4 3.178328616817842 5.2674169046119115 5 1.8589911845578604 3.8511127905602134 6 1.153642812195288 2.867883587628608 7 0.8216876024300857 2.383107744990317 8 0.5870593588292781 1.9458569352114259 9 0.4422679852199051 1.6491756582769246 >10 2.1621240045882346 16.19574384411871 >50 0.18112568712092958 5.224494163096199 >100 0.10699657192628109 8.274013010320086 >500 0.004595718809040123 1.255701961429649 >1k 0.0021542431917375574 1.2768256972163867 >5k 2.8723242556500766E-4 0.7464116667854579 >10k+ 1.4361621278250383E-4 0.5962843839968968 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10021 0.5951256825149094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6827 0.4054408776099477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5717 0.3395203599379041 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2597 0.1542302562110787 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1829 0.10862038452447553 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1727 0.10256282344109854 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9693926879298938E-5 0.0 12-13 0.0 0.0 0.0 5.9387853758597875E-5 0.0 14-15 0.0 0.0 0.0 5.9387853758597875E-5 0.0 16-17 0.0 0.0 0.0 5.9387853758597875E-5 0.0 18-19 0.0 0.0 0.0 5.9387853758597875E-5 0.0 20-21 0.0 0.0 0.0 8.908178063789681E-5 0.0 22-23 0.0 0.0 0.0 3.5632712255158724E-4 0.0 24-25 0.0 0.0 0.0 8.908178063789681E-4 0.0 26-27 0.0 0.0 0.0 0.0016925538321200394 0.0 28-29 0.0 0.0 0.0 0.0037117408599123673 0.0 30-31 0.0 0.0 0.0 0.009888077650806546 0.0 32-33 5.9387853758597875E-5 0.0 0.0 0.0195089099596994 0.0 34-35 5.9387853758597875E-5 0.0 0.0 0.03429648554559027 0.0 36-37 5.9387853758597875E-5 0.0 0.0 0.05903152663604629 0.0 38-39 5.9387853758597875E-5 0.0 0.0 0.09840567367799669 0.0 40-41 5.9387853758597875E-5 0.0 0.0 0.14158064336049736 0.0 42-43 5.9387853758597875E-5 0.0 0.0 0.18656694258263523 0.0 44-45 5.9387853758597875E-5 0.0 0.0 0.24322295506833758 0.0 46-47 5.9387853758597875E-5 0.0 0.0 0.3096779634242086 0.0 48-49 5.9387853758597875E-5 0.0 0.0 0.3851005376976279 0.0 50-51 5.9387853758597875E-5 0.0 0.0 0.4694906778885955 0.0 52-53 5.9387853758597875E-5 0.0 0.0 0.559433582405992 0.0 54-55 5.9387853758597875E-5 0.0 0.0 0.6600663005999361 0.0 56-57 5.9387853758597875E-5 0.0 0.0 0.7801782348267003 0.0 58-59 5.9387853758597875E-5 0.0 0.0 0.9150183567855967 0.0 60-61 5.9387853758597875E-5 0.0 0.0 1.0662792203087457 0.0 62-63 5.9387853758597875E-5 0.0 0.0 1.2260028529924947 0.0 64-65 5.9387853758597875E-5 0.0 0.0 1.3945158880325161 0.0 66-67 5.9387853758597875E-5 0.0 0.0 1.594860812687146 0.0 68-69 5.9387853758597875E-5 0.0 0.0 1.8167932221830263 0.0 70-71 5.9387853758597875E-5 0.0 0.0 2.073140892932014 0.0 72-73 5.9387853758597875E-5 0.0 0.0 2.3436822607293064 0.0 74-75 5.9387853758597875E-5 0.0 0.0 2.6270514049384563 0.0 76-77 5.9387853758597875E-5 0.0 0.0 2.9299294591073055 0.0 78-79 5.9387853758597875E-5 0.0 0.0 3.2672227745292624 0.0 80-81 5.9387853758597875E-5 0.0 0.0 3.635635325320724 0.0 82-83 5.9387853758597875E-5 0.0 0.0 4.020943720506507 0.0 84-85 5.9387853758597875E-5 0.0 0.0 4.4328875681030215 0.0 86-87 5.9387853758597875E-5 0.0 0.0 4.868853802544889 0.0 88-89 5.9387853758597875E-5 0.0 0.0 5.33763182618838 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9085 0.0 34.31671 1 GGTATCA 6005 0.0 29.39647 1 GTACATG 8765 0.0 27.827642 1 TACATGG 8670 0.0 27.345945 2 TATCAAC 11365 0.0 27.328377 2 ATCAACG 11395 0.0 27.089977 3 TCAACGC 11635 0.0 26.571936 4 CAACGCA 11720 0.0 26.338762 5 AACGCAG 11905 0.0 25.87193 6 ACATGGG 8955 0.0 25.575464 3 CATGGGG 4175 0.0 24.191628 4 ACGCAGA 13470 0.0 22.760487 7 AGTACTT 6900 0.0 22.660912 12-13 CGCAGAG 13610 0.0 22.527029 8 ATGGGGA 2715 0.0 21.656776 5 GCAGAGT 14230 0.0 21.31052 9 GAGTACT 6775 0.0 21.15576 12-13 TACTTTT 7575 0.0 20.459448 14-15 ATGGGAG 1945 0.0 19.99108 5 GTACTTT 7165 0.0 19.74499 14-15 >>END_MODULE