FastQCFastQC Report
Fri 27 May 2016
SRR939313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683846
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102660.6096757066857658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.448972174415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63430.3766971563907863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28350.16836456540562497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.117112847611955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.10196894490351255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.032.4579161
GTATCAA98900.031.1321541
GTACATG79550.026.5609721
TACATGG80100.026.1864722
ATCAACG122950.024.8567643
TATCAAC124700.024.6982122
ACATGGG82550.024.6619533
TCAACGC126450.024.1312264
CAACGCA127550.023.9231175
AACGCAG129700.023.526556
TAAGGTG5400.022.8489825
CATGGGA44100.021.7370224
AGTACTT78250.021.6209812-13
GAGTACT76850.021.33557512-13
CATGGGG40500.021.208544
ACGCAGA144700.020.9565417
CGCAGAG147200.020.600628
ATGGGAG15500.020.5130565
TAGCCCG1404.8837444E-420.3381025
CATTGCG2151.0891345E-619.8651269
Produced by FastQC (version 0.11.5)