##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939313_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683846 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12310626981327 33.0 31.0 34.0 30.0 34.0 2 32.34565274971702 34.0 31.0 34.0 30.0 34.0 3 32.4281739541502 34.0 31.0 34.0 30.0 34.0 4 36.01796185636929 37.0 35.0 37.0 35.0 37.0 5 35.949240607513985 37.0 35.0 37.0 35.0 37.0 6 35.98300379013283 37.0 35.0 37.0 35.0 37.0 7 35.91551246372887 37.0 35.0 37.0 35.0 37.0 8 35.88317458960024 37.0 35.0 37.0 35.0 37.0 9 37.552408593184886 39.0 37.0 39.0 35.0 39.0 10-11 37.45552710877361 39.0 37.0 39.0 34.5 39.0 12-13 37.360864354578744 39.0 37.0 39.0 34.0 39.0 14-15 38.56902976875558 40.0 38.0 41.0 34.0 41.0 16-17 38.57103618739481 40.0 38.0 41.0 34.0 41.0 18-19 38.68037278943561 40.0 38.0 41.0 34.0 41.0 20-21 38.65423500723938 40.0 38.0 41.0 34.0 41.0 22-23 38.55492069939888 40.0 38.0 41.0 34.0 41.0 24-25 38.45958834715289 40.0 38.0 41.0 34.0 41.0 26-27 38.264582093611885 40.0 38.0 41.0 33.5 41.0 28-29 38.05793641461274 40.0 37.5 41.0 33.0 41.0 30-31 37.80634660176762 40.0 37.0 41.0 33.0 41.0 32-33 37.70956667058626 40.0 37.0 41.0 33.0 41.0 34-35 37.581824881847865 40.0 36.5 41.0 33.0 41.0 36-37 37.38753870603368 40.0 36.0 41.0 32.0 41.0 38-39 37.22032656193025 40.0 36.0 41.0 31.5 41.0 40-41 36.98001361169608 39.5 35.5 41.0 31.0 41.0 42-43 36.72961036816906 39.0 35.0 41.0 31.0 41.0 44-45 36.41478199312763 39.0 35.0 41.0 30.0 41.0 46-47 36.02329666727242 39.0 35.0 40.0 29.5 41.0 48-49 35.631482332707385 39.0 35.0 40.0 28.0 41.0 50-51 35.32369676324319 38.0 35.0 40.0 27.0 41.0 52-53 35.008892737221814 38.0 34.0 40.0 26.5 41.0 54-55 34.70409170434826 38.0 34.0 40.0 26.0 41.0 56-57 34.32744413681536 37.5 33.5 40.0 25.5 41.0 58-59 33.94774551829562 37.0 33.0 40.0 24.0 41.0 60-61 33.55765105597543 36.0 33.0 39.0 23.5 41.0 62-63 33.13529087576892 36.0 32.0 39.0 22.5 40.0 64-65 32.757608177945016 35.0 32.0 39.0 22.0 40.0 66-67 32.28610751814596 35.0 31.0 38.0 20.5 40.0 68-69 31.77308791896646 35.0 31.0 37.5 19.5 40.0 70-71 31.249551027825586 35.0 30.5 37.0 18.0 39.0 72-73 30.722499266560007 34.0 30.0 36.5 14.0 39.0 74-75 30.16219862148914 34.0 29.0 36.0 9.0 38.5 76-77 28.309159804400167 31.5 27.0 34.5 7.0 36.0 78-79 29.291827756219988 33.5 29.0 35.0 7.0 37.0 80-81 29.29750879831053 34.0 29.0 35.0 2.0 36.5 82-83 28.91479327681985 34.0 29.0 35.0 2.0 36.0 84-85 28.41712335926207 33.5 28.5 35.0 2.0 36.0 86-87 27.725816969010232 33.0 26.5 35.0 2.0 35.0 88-89 27.05306453202965 32.5 25.5 35.0 2.0 35.0 90-91 26.457343486280813 32.0 25.0 34.0 2.0 35.0 92-93 25.97431623794575 32.0 24.0 34.0 2.0 35.0 94-95 25.294368665543047 31.5 21.5 34.0 2.0 35.0 96-97 24.35419361390531 31.0 12.5 34.0 2.0 35.0 98-99 23.101586784064576 31.0 2.0 34.0 2.0 35.0 100-101 18.840161451819228 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3286821800775286 1101 2 0.3118447831098692 1101 3 0.3972409661713385 1101 4 0.30598432991424573 1101 5 0.28557183952337795 1101 6 0.3065964116222446 1101 7 0.3286022636804944 1101 8 0.32620239431153664 1101 9 0.42996291127231956 1101 10-11 0.467134620523872 1101 12-13 0.4578556293968177 1101 14-15 0.5524110259526012 1101 16-17 0.5475752375016896 1101 18-19 0.5289431655065258 1101 20-21 0.5159296339443742 1101 22-23 0.4257317310546611 1101 24-25 0.5197765664882326 1101 26-27 0.4742607707371178 1101 28-29 0.49369592236340054 1101 30-31 0.451923323825433 1101 32-33 0.6690267265961864 1101 34-35 0.7055875396838971 1101 36-37 0.6322387220876848 1101 38-39 0.7401602719481275 1101 40-41 0.719222720636111 1101 42-43 0.7521469451125711 1101 44-45 0.7132696017728222 1101 46-47 0.562384124957994 1101 48-49 0.7275285798847619 1101 50-51 0.8411133072891701 1101 52-53 0.8221260374613664 1101 54-55 0.8498654181996415 1101 56-57 0.6898318226695608 1101 58-59 0.7272792164796442 1101 60-61 0.6813961777157331 1101 62-63 0.7826246150499543 1101 64-65 0.6441196782752954 1101 66-67 0.544645819626183 1101 68-69 0.8442865217527036 1101 70-71 0.5967707861113247 1101 72-73 0.6783348199770494 1101 74-75 0.7158729828921224 1101 76-77 0.7375727887631633 1101 78-79 0.6895715205731001 1101 80-81 0.8631104336325635 1101 82-83 0.9695753471321282 1101 84-85 1.102479393025046 1101 86-87 1.0626900124833405 1101 88-89 0.9625545623524694 1101 90-91 1.3188303745212266 1101 92-93 1.2455169804366157 1101 94-95 1.0968874172608345 1101 96-97 0.8549421867445623 1101 98-99 0.4384969716488243 1101 100-101 0.21675232083683582 1102 1 0.24112671653833928 1102 2 0.23689786166613658 1102 3 0.23683249471197598 1102 4 0.18857740514168597 1102 5 0.18987292788750665 1102 6 0.20640642489696148 1102 7 0.16283641014077688 1102 8 0.2152351264261938 1102 9 0.2573522582132526 1102 10-11 0.1741761050184394 1102 12-13 0.3012694229202211 1102 14-15 0.2688785719168081 1102 16-17 0.2822033628442142 1102 18-19 0.23421286140597886 1102 20-21 0.3498930087256653 1102 22-23 0.31808314154371686 1102 24-25 0.3201029539393687 1102 26-27 0.436206997998589 1102 28-29 0.3376068072225209 1102 30-31 0.43168648994608816 1102 32-33 0.4725889776953096 1102 34-35 0.572898050252924 1102 36-37 0.6815705209128495 1102 38-39 0.5959002661835697 1102 40-41 0.5251273294380141 1102 42-43 0.7174264651119273 1102 44-45 0.6405558362602832 1102 46-47 0.6428739497074147 1102 48-49 0.5900118850042517 1102 50-51 0.7333374510295556 1102 52-53 0.6680648682310988 1102 54-55 0.5497986386776148 1102 56-57 0.5598571542329296 1102 58-59 0.5363813262253956 1102 60-61 0.5573431681717409 1102 62-63 0.6934893794136414 1102 64-65 0.43683685380597836 1102 66-67 0.6593842022221068 1102 68-69 0.563620918686933 1102 70-71 0.5681798137284098 1102 72-73 0.784741594546432 1102 74-75 0.7461752566435074 1102 76-77 0.8034425666654315 1102 78-79 0.7175821809393561 1102 80-81 1.0253992865268202 1102 82-83 1.0536980590708431 1102 84-85 0.8154949045480677 1102 86-87 0.734760543025331 1102 88-89 0.8319287428317388 1102 90-91 1.0405877111346697 1102 92-93 0.853300143877977 1102 94-95 0.879075146878872 1102 96-97 0.31303042502364065 1102 98-99 0.03778675417897759 1102 100-101 0.07146403221939224 1103 1 0.18458267050567656 1103 2 0.18580511580341152 1103 3 0.2520380238471063 1103 4 0.1319428912434546 1103 5 0.24813341828853908 1103 6 0.2066537852320991 1103 7 0.2547150853978124 1103 8 0.1792488840619626 1103 9 0.24441739100133475 1103 10-11 0.22508037616122323 1103 12-13 0.23427409709326952 1103 14-15 0.21355991719841683 1103 16-17 0.19364245392742419 1103 18-19 0.1868826822225529 1103 20-21 0.26037301817996905 1103 22-23 0.32915445222747763 1103 24-25 0.3031581597130071 1103 26-27 0.21192330381257563 1103 28-29 0.2460052407429032 1103 30-31 0.32948632775595854 1103 32-33 0.389720005320612 1103 34-35 0.3597366058641711 1103 36-37 0.33780139327573266 1103 38-39 0.3587970309385966 1103 40-41 0.3966882261294131 1103 42-43 0.3772697356475163 1103 44-45 0.4362150087645702 1103 46-47 0.544012948285193 1103 48-49 0.5636816609944049 1103 50-51 0.37614118058768753 1103 52-53 0.1805753107493615 1103 54-55 0.17464408940726628 1103 56-57 0.46165611996516986 1103 58-59 0.3822748186517657 1103 60-61 0.33307842333595516 1103 62-63 0.34414343763094024 1103 64-65 0.3508402198625973 1103 66-67 0.32594104416007497 1103 68-69 0.26934729006974223 1103 70-71 0.18463192484903246 1103 72-73 0.37260749019867845 1103 74-75 0.18807553008466726 1103 76-77 0.24589440882753166 1103 78-79 0.3521688202211557 1103 80-81 0.509136668908404 1103 82-83 0.5051249655181067 1103 84-85 0.41239230502322854 1103 86-87 0.361215669741469 1103 88-89 0.6628074221639082 1103 90-91 0.7974265927562456 1103 92-93 0.3470413568808972 1103 94-95 0.2599018683152714 1103 96-97 3.732778585039398E-4 1103 98-99 -0.260997431060094 1103 100-101 -0.34075524232612864 1104 1 0.09989650275262818 1104 2 0.09876722535409499 1104 3 0.13143582447170132 1104 4 0.07612233797891577 1104 5 0.1546172048629728 1104 6 0.12465388658580423 1104 7 0.18450428552704068 1104 8 0.18803735887873785 1104 9 0.2647772124921204 1104 10-11 0.22183210867613212 1104 12-13 0.15309314430509602 1104 14-15 0.16883442737496068 1104 16-17 0.1120612331853863 1104 18-19 0.07773701477673 1104 20-21 0.19950477324606908 1104 22-23 0.2265427335220167 1104 24-25 0.22991526080699742 1104 26-27 0.3208029177816485 1104 28-29 0.22710198285473382 1104 30-31 0.2271238362284862 1104 32-33 0.25650105122750233 1104 34-35 0.25375744988715354 1104 36-37 0.31119505581857254 1104 38-39 0.4264561720926352 1104 40-41 0.4455228960647446 1104 42-43 0.45293641339974755 1104 44-45 0.4261107173853347 1104 46-47 0.3713842290793039 1104 48-49 0.3040403897655608 1104 50-51 0.3130932371177195 1104 52-53 0.31273638554206684 1104 54-55 0.3239200834654099 1104 56-57 0.210976139539099 1104 58-59 0.15713359651611825 1104 60-61 0.27151277780786387 1104 62-63 0.10237149772101617 1104 64-65 0.22400852877601807 1104 66-67 0.1501812476909805 1104 68-69 0.20485901542654616 1104 70-71 0.1603674122653338 1104 72-73 0.03585703252335293 1104 74-75 -0.12106712842302159 1104 76-77 -0.07985857793947204 1104 78-79 0.30368453321279887 1104 80-81 0.38804512117397394 1104 82-83 0.2900672493338199 1104 84-85 0.14914684060154926 1104 86-87 0.20166553766492257 1104 88-89 0.5514288058953731 1104 90-91 0.47258895664138834 1104 92-93 0.3212098209921841 1104 94-95 0.5202367789656499 1104 96-97 0.31956539380714233 1104 98-99 0.4732080192354182 1104 100-101 0.01358226076199287 1105 1 0.2142967312370132 1105 2 0.16210119336628281 1105 3 0.1528638518898262 1105 4 0.1814201292965123 1105 5 0.16074210966114322 1105 6 0.22862494523013055 1105 7 0.26513528324219493 1105 8 0.13405335278581987 1105 9 0.2533914546855698 1105 10-11 0.28380373853142515 1105 12-13 0.22889607652139432 1105 14-15 0.2584581897512024 1105 16-17 0.3149627944953579 1105 18-19 0.4029811122633973 1105 20-21 0.3445818867266439 1105 22-23 0.24329235271471106 1105 24-25 0.30574332630585843 1105 26-27 0.27568372509315253 1105 28-29 0.2891697665561779 1105 30-31 0.3754787486428057 1105 32-33 0.2414532599099104 1105 34-35 0.34893528690162157 1105 36-37 0.24408595452383253 1105 38-39 0.31441276005912044 1105 40-41 0.4260739242444842 1105 42-43 0.5025318056312784 1105 44-45 0.2745039009344623 1105 46-47 0.38925513540070256 1105 48-49 0.42200992518063174 1105 50-51 0.5705574412304415 1105 52-53 0.6202441920919455 1105 54-55 0.6428677224600818 1105 56-57 0.5068331463936389 1105 58-59 0.5215915173082024 1105 60-61 0.45447888595714403 1105 62-63 0.5467662044993844 1105 64-65 0.5073160306800517 1105 66-67 0.4466757259849672 1105 68-69 0.6340589392650848 1105 70-71 0.7055595295539874 1105 72-73 0.617893970832565 1105 74-75 0.28490431102861535 1105 76-77 0.7365087107124708 1105 78-79 0.7423082955890372 1105 80-81 0.8356864006865408 1105 82-83 0.7539253986102885 1105 84-85 0.84298633031975 1105 86-87 0.8857306557151858 1105 88-89 0.8375563763447254 1105 90-91 0.942771934554564 1105 92-93 1.1475451218299604 1105 94-95 1.1925459945025843 1105 96-97 1.1473224387424032 1105 98-99 1.278224774757124 1105 100-101 0.6101391914550653 1106 1 0.038309381223065486 1106 2 0.06839456142506606 1106 3 0.08979809056529575 1106 4 0.04180409357890369 1106 5 -0.032457034604505 1106 6 0.07119512474831424 1106 7 0.0205894735500749 1106 8 0.025366905013520125 1106 9 0.06364389631890788 1106 10-11 0.13199925522754086 1106 12-13 0.13564930877885928 1106 14-15 0.15464440498698906 1106 16-17 0.06397386323315146 1106 18-19 0.007021108542815568 1106 20-21 0.08993952787630377 1106 22-23 0.1417509147089433 1106 24-25 0.11170909693184683 1106 26-27 0.23467433061821197 1106 28-29 0.15718297462984765 1106 30-31 0.1438184379740335 1106 32-33 0.052571109412284045 1106 34-35 0.2857493507096436 1106 36-37 0.163876299607999 1106 38-39 0.3205727451767828 1106 40-41 0.2675722034743231 1106 42-43 0.26336335618471196 1106 44-45 0.37762751734224054 1106 46-47 0.24337996099831827 1106 48-49 0.20418944336499578 1106 50-51 0.47068524329535677 1106 52-53 0.5775897413431821 1106 54-55 0.5696890414071518 1106 56-57 0.6613436105365054 1106 58-59 0.4728514931244021 1106 60-61 0.5469499119082144 1106 62-63 0.6619491642240618 1106 64-65 0.6632291550405824 1106 66-67 0.611657402108122 1106 68-69 0.6019499261500236 1106 70-71 0.550842572991808 1106 72-73 0.6420494912492707 1106 74-75 0.5833690119866652 1106 76-77 0.6437528740461147 1106 78-79 0.8374086583459892 1106 80-81 0.7291608681779422 1106 82-83 0.9436841187293474 1106 84-85 0.6589606582892387 1106 86-87 0.7738824555477635 1106 88-89 0.9456164637784532 1106 90-91 0.7529395468696976 1106 92-93 0.9333423499947813 1106 94-95 1.1805951062131257 1106 96-97 1.2614569378587674 1106 98-99 1.4483043952575407 1106 100-101 1.1156506787585663 1107 1 0.0388758099753872 1107 2 0.0327887359122343 1107 3 0.10166348883105769 1107 4 0.039059339643685576 1107 5 0.11320682488692313 1107 6 0.1434204977601965 1107 7 0.16234789953445983 1107 8 0.12647087037147742 1107 9 0.21589837549337432 1107 10-11 0.16208828584252188 1107 12-13 0.13226072367364594 1107 14-15 0.17990951718483217 1107 16-17 0.16918925240324256 1107 18-19 0.13392914947691992 1107 20-21 0.20833954499757823 1107 22-23 0.23721021066796055 1107 24-25 0.25108392081459385 1107 26-27 0.30063403142398926 1107 28-29 0.33349016592082137 1107 30-31 0.4066428058245677 1107 32-33 0.27208543599341084 1107 34-35 0.4084578646188888 1107 36-37 0.3923074203736121 1107 38-39 0.1436916345769319 1107 40-41 0.20597771367039286 1107 42-43 0.37019688391582406 1107 44-45 0.21017987098211677 1107 46-47 0.21724968951915002 1107 48-49 0.13221732250308094 1107 50-51 0.3374437584530412 1107 52-53 0.21600185036288622 1107 54-55 0.325847527315986 1107 56-57 0.2455315807026892 1107 58-59 0.3335207208480213 1107 60-61 0.5273157364223096 1107 62-63 0.51434596397295 1107 64-65 0.5127401287059214 1107 66-67 0.6467383536228226 1107 68-69 0.713743985781985 1107 70-71 0.7006845579179561 1107 72-73 0.7585048172133249 1107 74-75 0.7978174038355128 1107 76-77 0.7150709979827212 1107 78-79 0.8199956112232556 1107 80-81 0.8474649348367578 1107 82-83 1.124533975873728 1107 84-85 0.9011392688266717 1107 86-87 1.2750748808116263 1107 88-89 1.492112310086501 1107 90-91 1.4495302735606899 1107 92-93 1.3712782736339086 1107 94-95 1.6012394367535485 1107 96-97 1.5525913291440396 1107 98-99 1.3998567239288953 1107 100-101 0.7533722298031442 1108 1 0.06822667151689643 1108 2 -0.0025623238812428895 1108 3 0.026392686901857587 1108 4 0.06517732690770828 1108 5 0.09274175312312849 1108 6 0.08189778594677222 1108 7 0.004369796122134062 1108 8 -0.03725175462125918 1108 9 0.06391431768583544 1108 10-11 0.04978555509261895 1108 12-13 0.012934404358098561 1108 14-15 -2.6255184787515873E-4 1108 16-17 -0.016743061641548707 1108 18-19 0.1421687403942471 1108 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2316 44-45 -1.1731820461933395 2316 46-47 -1.0112266773601846 2316 48-49 -0.9318219173953963 2316 50-51 -0.9421748187576355 2316 52-53 -1.144090532614868 2316 54-55 -1.0278466484403452 2316 56-57 -0.9207852197363593 2316 58-59 -0.906181092510927 2316 60-61 -0.9231724756011985 2316 62-63 -0.8366979179414855 2316 64-65 -0.7012842475946428 2316 66-67 -0.6186774841124283 2316 68-69 -0.38494801760234054 2316 70-71 -0.47606877495804056 2316 72-73 -0.3681970647468411 2316 74-75 -0.3945701473295671 2316 76-77 -0.6370567832251197 2316 78-79 -0.6703842108709104 2316 80-81 -0.4997212717443844 2316 82-83 -0.292650630012254 2316 84-85 -0.19495046157053153 2316 86-87 -0.48657703659250373 2316 88-89 -1.0434962970800434 2316 90-91 -1.0593604446940965 2316 92-93 -0.7806528507401076 2316 94-95 -0.4076535682086053 2316 96-97 -0.18269942569806474 2316 98-99 0.0932229858483602 2316 100-101 0.6177567625144924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 21.0 5 56.0 6 149.0 7 339.0 8 799.0 9 1315.0 10 2233.0 11 3155.0 12 4652.0 13 6360.0 14 7879.0 15 10135.0 16 13448.0 17 18009.0 18 17153.0 19 12571.0 20 12156.0 21 13216.0 22 13935.0 23 15741.0 24 17978.0 25 21507.0 26 25035.0 27 29551.0 28 35907.0 29 42793.0 30 51653.0 31 62740.0 32 77751.0 33 99395.0 34 134130.0 35 191129.0 36 265889.0 37 320308.0 38 150142.0 39 4607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.259923542965836 19.16863248631867 14.734029216616154 22.837414754099335 2 16.526808271065168 20.983272817110354 38.26044662041541 24.22947229140907 3 19.569782509801964 24.636457253216744 29.542962955044583 26.250797281936713 4 13.78754351644984 16.720947165002027 34.96911237726015 34.52239694128798 5 14.492002237734331 37.18053788766906 32.785836709532816 15.54162316506379 6 33.30981574324493 35.15196757898288 16.443012009411788 15.09520466836041 7 29.611496538282005 32.68838124151496 19.599476436681265 18.10064578352177 8 27.186809245025973 34.53219593715815 18.981427042615536 19.299567775200344 9 27.30540678898189 16.38190190789419 19.337160286629537 36.97553101649439 10-11 25.94073329746307 26.19657617145511 26.01271137621849 21.84997915486333 12-13 26.6779741140223 23.430141473745223 27.189244147030074 22.7026402652024 14-15 23.394477705277676 24.721304835586036 25.567857543729204 26.31635991540709 16-17 22.98681727547128 28.110100024794626 26.056926683622034 22.846156016112054 18-19 22.744277904356426 25.93272363764965 29.104035970045945 22.218962487947973 20-21 23.46055502122968 25.248634747071506 28.940214526807832 22.350595704890978 22-23 23.082633447476788 25.093862502865466 29.152012713751734 22.671491335906016 24-25 23.02870927626398 25.394899533567795 29.12338776824009 22.453003421928134 26-27 22.75893995056555 25.68744410118265 29.036800277460053 22.516815670791747 28-29 22.558119923080852 25.80607133906545 29.334184955156235 22.30162378269747 30-31 22.69376178106549 25.60142079501332 29.011233806416975 22.693583617504213 32-33 22.483914799809483 25.637974019001735 28.991695202530398 22.88641597865838 34-35 23.095399859129667 25.69829399614448 28.59044148476854 22.61586465995731 36-37 23.222750644772848 25.476552837223252 28.614877957157685 22.685818560846215 38-39 22.926476409254864 25.5371386201443 28.80105285256946 22.735332118031376 40-41 22.804002740236125 25.54079427056654 28.973334960608366 22.681868028588966 42-43 22.960874263328385 25.682687879285478 28.332493487358214 23.02394437002792 44-45 22.86295276021937 25.970758829082246 28.1368396765815 23.029448734116887 46-47 22.91822003436784 26.247749565502453 27.738596536550503 23.095433863579203 48-49 23.308612046638665 26.08598565539817 27.55982014907601 23.045582148887156 50-51 23.158092592224165 26.392445150104333 27.387964038268358 23.061498219403145 52-53 23.446628565387975 26.337155041837267 27.164814111508452 23.051402281266302 54-55 22.848526527960395 26.31256064984565 27.27669276168961 23.562220060504345 56-57 23.07772481985282 26.667810882502195 27.117469889356506 23.136994408288476 58-59 23.10845417054676 26.096532383739714 27.33678661700146 23.45822682871206 60-61 23.099949698219007 26.202001737697888 27.687181122638354 23.01086744144475 62-63 23.06645468885109 26.318635439333327 27.469913720198786 23.144996151616795 64-65 22.84574208263169 26.117805938846526 27.47668951812978 23.559762460392 66-67 22.88233533431997 26.558881031169307 27.29654194351469 23.262241690996035 68-69 22.81724285276929 26.28368352489716 27.56048536167688 23.33858826065667 70-71 22.728355205879872 26.470740198331676 27.40701940676285 23.3938851890256 72-73 22.98045664508512 26.3470649928794 27.087215814272803 23.58526254776268 74-75 22.830582040087428 26.729516888287485 26.728032190620926 23.711868881004154 76-77 22.774345056777573 26.475968323511456 27.08123656542943 23.668450054281546 78-79 22.718418522391932 26.59974831382736 27.202506409445537 23.479326754335172 80-81 22.665869680959258 27.011193729587262 26.99150663852868 23.331429950924804 82-83 22.827770473071766 26.675479824164444 26.835025293286918 23.661724409476875 84-85 22.805678191473568 26.45280506649658 26.711082842492722 24.030433899537133 86-87 22.79224720577316 26.306276153612473 26.908489182458656 23.992987458155707 88-89 22.602561741474023 26.294504312978034 26.934760880487623 24.16817306506032 90-91 22.47847814469177 26.531330432491412 27.09352610087844 23.896665321938375 92-93 22.51963659384528 26.15978539605166 27.051078305260695 24.269499704842367 94-95 22.41627203437844 26.041217546022615 27.125045877117028 24.417464542481916 96-97 22.586269765762427 25.883869427489216 27.112069631070774 24.417791175677586 98-99 22.303329581998344 26.016319791904834 26.84059399766606 24.839756628430763 100-101 22.53762318231205 25.349536816393286 27.557442517395554 24.55539748389911 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2529.0 1 1675.5 2 1093.5 3 1406.5 4 1185.5 5 1071.0 6 1910.5 7 3826.0 8 5637.0 9 5737.0 10 4341.0 11 3143.0 12 2839.0 13 2891.5 14 3089.5 15 3123.5 16 3052.5 17 3105.5 18 3165.0 19 3278.0 20 3464.5 21 3864.5 22 4400.5 23 5147.0 24 5974.0 25 6737.5 26 7967.5 27 9484.0 28 10574.5 29 12078.5 30 14255.0 31 16340.0 32 18768.0 33 20088.5 34 21102.0 35 23153.5 36 25883.5 37 29309.0 38 32637.5 39 34249.0 40 35816.0 41 40546.0 42 45004.0 43 48286.0 44 52207.5 45 56261.5 46 57572.5 47 59517.5 48 68697.5 49 72030.5 50 68250.0 51 66302.0 52 65140.0 53 66797.5 54 68206.0 55 64575.5 56 57188.0 57 51648.5 58 47549.5 59 41884.0 60 36088.0 61 30563.0 62 25663.0 63 20845.5 64 16599.5 65 13104.5 66 9998.0 67 8575.0 68 6704.5 69 4584.5 70 3428.0 71 2647.5 72 2172.0 73 1859.0 74 1552.0 75 1282.0 76 893.5 77 507.5 78 330.5 79 231.0 80 153.0 81 106.0 82 72.0 83 43.0 84 22.0 85 8.0 86 7.0 87 6.0 88 3.0 89 1.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2810827118394438 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001009593513896164 16-17 8.017360257410713E-4 18-19 2.9693926879298938E-5 20-21 2.9693926879298938E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016628599052407405 36-37 0.0067405214016008595 38-39 0.0023458202234646164 40-41 0.002939698761050595 42-43 3.266331956722883E-4 44-45 0.0012768388558098544 46-47 2.6724534191369044E-4 48-49 4.157149763101851E-4 50-51 2.9693926879298938E-5 52-53 2.9693926879298938E-5 54-55 0.0 56-57 8.611238794996692E-4 58-59 0.002256738442826719 60-61 5.344906838273809E-4 62-63 0.0013065327826891533 64-65 2.9693926879298938E-5 66-67 5.938785375859787E-4 68-69 0.0017519416858786374 70-71 0.0 72-73 0.0 74-75 8.908178063789682E-5 76-77 0.2480927590765426 78-79 1.7816356127579365E-4 80-81 8.908178063789682E-5 82-83 0.0 84-85 0.0 86-87 0.10196894490351255 88-89 0.3565052861128631 90-91 0.26418686744512265 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.9387853758597875E-5 100-101 6.235724644652777E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1683846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.823054412771576 #Duplication Level Percentage of deduplicated Percentage of total 1 67.73345488103944 27.65086514173681 2 15.065852324569045 12.300682184413265 3 6.412919858489379 7.853849289835659 4 3.2783341019097625 5.353264457020274 5 1.9230378640997254 3.925213968198156 6 1.2193687670861675 2.9867014516795685 7 0.8275086988207228 2.3647002847300116 8 0.5653954624421321 1.8464935782407443 9 0.4320376944320075 1.5873388477349597 >10 2.244450272332992 16.576717017808242 >50 0.18192420620415856 5.191911221950732 >100 0.10843829109511353 8.401241205651026 >500 0.00465764958684904 1.2104754805901576 >1k 0.0021832732438354873 1.3144580100930428 >5k 2.91103099178065E-4 0.8260966329592069 >10k+ 1.455515495890325E-4 0.6099912273580679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10266 0.6096757066857658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7560 0.448972174415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6343 0.3766971563907863 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2835 0.16836456540562497 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1972 0.117112847611955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1717 0.10196894490351255 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9693926879298938E-5 0.0 12-13 0.0 0.0 0.0 5.9387853758597875E-5 0.0 14-15 0.0 0.0 0.0 5.9387853758597875E-5 0.0 16-17 0.0 0.0 0.0 5.9387853758597875E-5 0.0 18-19 0.0 0.0 0.0 5.9387853758597875E-5 0.0 20-21 0.0 0.0 0.0 8.908178063789681E-5 0.0 22-23 0.0 0.0 0.0 3.5632712255158724E-4 0.0 24-25 0.0 0.0 0.0 8.908178063789681E-4 0.0 26-27 0.0 0.0 0.0 0.0016331659783614416 0.0 28-29 0.0 0.0 0.0 0.0036523530061537694 0.0 30-31 0.0 0.0 0.0 0.009828689797047948 0.0 32-33 0.0 0.0 0.0 0.019419828179061505 0.0 34-35 0.0 0.0 0.0 0.034266791618710976 0.0 36-37 0.0 0.0 0.0 0.059269078051080684 0.0 38-39 0.0 0.0 0.0 0.09849475545863458 0.0 40-41 0.0 0.0 0.0 0.14149156157985943 0.0 42-43 0.0 0.0 0.0 0.18686388185142822 0.0 44-45 0.0 0.0 0.0 0.2435495882640099 0.0 46-47 0.0 0.0 0.0 0.3096779634242086 0.0 48-49 0.0 0.0 0.0 0.38501145591699004 0.0 50-51 0.0 0.0 0.0 0.4693719021810783 0.0 52-53 0.0 0.0 0.0 0.558839703868406 0.0 54-55 0.0 0.0 0.0 0.6595021159892294 0.0 56-57 0.0 0.0 0.0 0.7799109894847867 0.0 58-59 0.0 0.0 0.0 0.9150480507124761 0.0 60-61 0.0 0.0 0.0 1.0663683020893835 0.0 62-63 0.0 0.0 0.0 1.2256168319430638 0.0 64-65 0.0 0.0 0.0 1.394634663740033 0.0 66-67 0.0 0.0 0.0 1.5935542799044569 0.0 68-69 0.0 0.0 0.0 1.8130220934693553 0.0 70-71 0.0 0.0 0.0 2.0662222079691372 0.0 72-73 0.0 0.0 0.0 2.3342395919816896 0.0 74-75 0.0 0.0 0.0 2.6146987313566683 0.0 76-77 0.0 0.0 0.0 2.91362749325057 0.0 78-79 0.0 0.0 0.0 3.246912128543822 0.0 80-81 0.0 0.0 0.0 3.6107518145958717 0.0 82-83 0.0 5.9387853758597875E-5 0.0 3.991012242212174 0.0 84-85 0.0 5.9387853758597875E-5 0.0 4.39565138379638 0.0 86-87 0.0 5.9387853758597875E-5 0.0 4.822234337344389 0.0 88-89 0.0 5.9387853758597875E-5 0.0 5.282816837169195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6715 0.0 32.457916 1 GTATCAA 9890 0.0 31.132154 1 GTACATG 7955 0.0 26.560972 1 TACATGG 8010 0.0 26.186472 2 ATCAACG 12295 0.0 24.856764 3 TATCAAC 12470 0.0 24.698212 2 ACATGGG 8255 0.0 24.661953 3 TCAACGC 12645 0.0 24.131226 4 CAACGCA 12755 0.0 23.923117 5 AACGCAG 12970 0.0 23.52655 6 TAAGGTG 540 0.0 22.848982 5 CATGGGA 4410 0.0 21.737022 4 AGTACTT 7825 0.0 21.62098 12-13 GAGTACT 7685 0.0 21.335575 12-13 CATGGGG 4050 0.0 21.20854 4 ACGCAGA 14470 0.0 20.956541 7 CGCAGAG 14720 0.0 20.60062 8 ATGGGAG 1550 0.0 20.513056 5 TAGCCCG 140 4.8837444E-4 20.338102 5 CATTGCG 215 1.0891345E-6 19.865126 9 >>END_MODULE