FastQCFastQC Report
Fri 27 May 2016
SRR939312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712805
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103780.6059066852327031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68230.39835241022766743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56800.33161976990959274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26880.1569355530839763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.10795157650754171No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89800.035.123531
GGTATCA58450.031.0445461
ATCAACG112250.028.0975513
TCAACGC114350.027.6230264
GTACATG86700.027.6132721
TATCAAC114450.027.5988882
CAACGCA115400.027.3305875
TACATGG86450.027.2113342
AACGCAG117000.026.9823886
ACATGGG89500.025.5951233
CATGGGG45950.023.842934
ACGCAGA132600.023.807997
CGCAGAG133850.023.5856518
AGTACTT71100.022.73404912-13
GAGTACT69350.022.31663512-13
GCAGAGT141950.022.0387959
TAGGCCG1100.002903441721.5580985
GGGCGTA5300.020.569869
GTACTTT75100.020.54515514-15
ATGGGGA26450.020.4414795
Produced by FastQC (version 0.11.5)