FastQCFastQC Report
Fri 27 May 2016
SRR939312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712805
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105760.6174666701696924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77790.45416728699414116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64910.3789690011414026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29530.17240725009560343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.1113962184837153No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18270.10666713373676513No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.10532430720368051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99500.032.7972031
GGTATCA66850.031.6660841
GTACATG84300.026.8041361
TACATGG85350.026.0217932
ATCAACG126100.025.7039893
TATCAAC128150.025.3668732
TCAACGC128300.025.2632374
CAACGCA129400.025.048485
ACATGGG86850.024.643463
AACGCAG132650.024.506336
CATGGGG42350.023.7562264
GAGTACT80950.022.60034812-13
TATAACG1050.002208407322.5982952
AGTACTT83300.022.0767112-13
ACGCAGA149450.021.6245027
CGCAGAG150250.021.4461948
GTACTTT85600.021.28948214-15
GTTTACG1404.8049176E-420.3872661
TATACGG1404.883208E-420.3384655
GCAGAGT157850.020.2037649
Produced by FastQC (version 0.11.5)