##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939311_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1981632 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90959572715822 33.0 31.0 34.0 30.0 34.0 2 31.93581653909505 33.0 31.0 34.0 30.0 34.0 3 31.989865424054518 34.0 31.0 34.0 30.0 34.0 4 35.478490960985695 37.0 35.0 37.0 33.0 37.0 5 35.52974820753803 37.0 35.0 37.0 33.0 37.0 6 35.64425685495591 37.0 35.0 37.0 33.0 37.0 7 35.590515797080386 37.0 35.0 37.0 33.0 37.0 8 35.58183911038982 37.0 35.0 37.0 33.0 37.0 9 37.18288965862481 39.0 37.0 39.0 34.0 39.0 10-11 37.08573261836708 39.0 37.0 39.0 33.0 39.0 12-13 36.99178530625262 39.0 37.0 39.0 33.0 39.0 14-15 38.16060600555502 40.0 38.0 41.0 33.0 41.0 16-17 38.12293654926848 40.0 38.0 41.0 33.0 41.0 18-19 38.09164895399347 40.0 38.0 41.0 33.0 41.0 20-21 38.07618493241934 40.0 38.0 41.0 33.0 41.0 22-23 38.072570487355875 40.0 38.0 41.0 33.0 41.0 24-25 37.96861576720602 40.0 38.0 41.0 33.0 41.0 26-27 37.8555475991506 40.0 38.0 41.0 33.0 41.0 28-29 37.725072061815716 40.0 37.5 41.0 32.5 41.0 30-31 37.54647911418467 40.0 37.0 41.0 32.0 41.0 32-33 37.30468850926913 40.0 36.5 41.0 31.5 41.0 34-35 36.97928374188548 40.0 36.0 41.0 31.0 41.0 36-37 36.71149663509673 39.5 35.5 41.0 30.0 41.0 38-39 36.12301754311598 38.5 35.0 40.5 29.5 41.0 40-41 36.12205596195459 39.0 35.0 40.0 30.0 41.0 42-43 36.25749735571489 39.0 35.0 41.0 30.0 41.0 44-45 36.115206809336954 39.0 35.0 41.0 29.5 41.0 46-47 35.774287304605494 39.0 35.0 41.0 27.5 41.0 48-49 35.4645277226044 39.0 35.0 41.0 26.0 41.0 50-51 34.28708155701967 38.0 34.0 40.0 24.5 40.5 52-53 34.419938212544004 38.0 34.0 39.5 24.0 40.5 54-55 34.927777205858604 39.0 35.0 40.0 25.0 41.0 56-57 34.826834144785714 38.5 35.0 40.5 24.5 41.0 58-59 34.612713409957045 38.0 34.0 40.0 24.0 41.0 60-61 34.26632669436101 38.0 34.0 40.0 23.0 41.0 62-63 33.907894351726256 37.5 34.0 40.0 22.5 41.0 64-65 33.51843127280948 37.0 33.0 40.0 22.0 41.0 66-67 33.115073838129376 36.0 33.0 39.0 20.5 41.0 68-69 32.68762060766076 36.0 32.5 39.0 19.0 40.5 70-71 32.2157249681071 35.0 32.0 38.5 17.5 40.0 72-73 31.763017300891384 35.0 31.5 37.5 14.0 40.0 74-75 31.312184855714886 35.0 31.0 37.0 9.5 39.0 76-77 30.858055380615575 35.0 31.0 36.5 7.0 39.0 78-79 30.453434845622194 35.0 31.0 36.0 4.0 38.5 80-81 30.0160087745858 34.0 30.0 35.5 2.0 37.0 82-83 29.573973371443337 34.0 30.0 35.0 2.0 37.0 84-85 29.13948124576107 34.0 29.0 35.0 2.0 36.5 86-87 28.747888356667637 34.0 29.0 35.0 2.0 36.0 88-89 28.294721976633404 34.0 29.0 35.0 2.0 36.0 90-91 27.846439197590673 34.0 27.5 35.0 2.0 35.0 92-93 27.385749725478796 34.0 26.5 35.0 2.0 35.0 94-95 26.864367602057293 33.0 25.5 35.0 2.0 35.0 96-97 26.227867232664792 33.0 24.5 35.0 2.0 35.0 98-99 25.450326044391694 33.0 21.0 35.0 2.0 35.0 100-101 22.771686670380777 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5334477441453451 1101 2 0.5716124978880117 1101 3 0.6280934114576979 1101 4 0.549239918049345 1101 5 0.5283999100841115 1101 6 0.568525762996849 1101 7 0.48596219901413207 1101 8 0.4035561919353725 1101 9 0.5411019341416718 1101 10-11 0.6924187893071476 1101 12-13 0.744601073760883 1101 14-15 0.8922159098630758 1101 16-17 0.8863842924340659 1101 18-19 0.9745967551100208 1101 20-21 1.0718318262166733 1101 22-23 1.1005464861978353 1101 24-25 1.0303392164897858 1101 26-27 1.0106801813283042 1101 28-29 1.181668692130451 1101 30-31 1.0785772072239297 1101 32-33 1.0567324499033433 1101 34-35 1.0359934733649254 1101 36-37 0.9315181202937666 1101 38-39 1.341399186321219 1101 40-41 1.1067703163253597 1101 42-43 0.9959100808999963 1101 44-45 0.989734996726682 1101 46-47 1.0757806151112277 1101 48-49 1.1432304410743939 1101 50-51 1.0067683180028055 1101 52-53 0.9340553006218428 1101 54-55 0.9575488762932451 1101 56-57 0.9301035465271426 1101 58-59 0.9220748330401491 1101 60-61 0.9262008313199033 1101 62-63 0.9451887682658437 1101 64-65 0.8821614459600369 1101 66-67 0.9145288266345162 1101 68-69 0.9880737121568828 1101 70-71 1.025288732875552 1101 72-73 0.9759352344085208 1101 74-75 0.9516127760944357 1101 76-77 0.9179240665909454 1101 78-79 0.9584534277159058 1101 80-81 0.9209410130147084 1101 82-83 0.8856276633517979 1101 84-85 0.8150548527544785 1101 86-87 0.9539068720197577 1101 88-89 0.7939270478749272 1101 90-91 0.8609895517729278 1101 92-93 0.9521007895129934 1101 94-95 1.0096773162207775 1101 96-97 0.8136867805733807 1101 98-99 0.9702578249776863 1101 100-101 0.6516382035592123 1102 1 0.27678617034206 1102 2 0.27399167718409245 1102 3 0.34152038903562243 1102 4 0.27818451170690395 1102 5 0.20688866123444427 1102 6 0.20478057187309417 1102 7 0.3243951358011401 1102 8 0.2322886870668981 1102 9 0.42353602693305703 1102 10-11 0.3750715891819496 1102 12-13 0.29508091749644905 1102 14-15 0.6366375147824712 1102 16-17 0.4982648675823995 1102 18-19 0.5370418687394007 1102 20-21 0.47370214659360954 1102 22-23 0.5661251081393672 1102 24-25 0.5851902428382019 1102 26-27 0.47622165068349886 1102 28-29 0.2946661463563274 1102 30-31 0.2670604972728583 1102 32-33 0.12321371530070024 1102 34-35 0.2180523250343782 1102 36-37 0.257566587650949 1102 38-39 0.7492214825417491 1102 40-41 0.55235081891108 1102 42-43 0.3597639351191688 1102 44-45 0.5298653792898236 1102 46-47 0.5075556440359961 1102 48-49 0.483931000763306 1102 50-51 0.47393134979851936 1102 52-53 0.37425887049221274 1102 54-55 0.4519922631493145 1102 56-57 0.26450049593813674 1102 58-59 0.2893322587078515 1102 60-61 0.42751613151115464 1102 62-63 0.33826346041720967 1102 64-65 0.4833624608503371 1102 66-67 0.5268121621213808 1102 68-69 0.3858056289574492 1102 70-71 0.4631161811405775 1102 72-73 0.3631424481914074 1102 74-75 0.29752209550837705 1102 76-77 0.1301391465298316 1102 78-79 0.22085047313181505 1102 80-81 0.2111373989557741 1102 82-83 0.0068287703015990076 1102 84-85 -0.015476583758491813 1102 86-87 -0.02150666421272618 1102 88-89 -0.09789859405107748 1102 90-91 0.03425425474457455 1102 92-93 0.022068479914242545 1102 94-95 0.10607414003630922 1102 96-97 0.09882646936621953 1102 98-99 0.31646173481065887 1102 100-101 -0.015406893433695501 1103 1 0.24326720905154176 1103 2 0.3951744501025871 1103 3 0.46874111480057934 1103 4 0.40346825009254417 1103 5 0.290269914828464 1103 6 0.3848447345800352 1103 7 0.3662370897371616 1103 8 0.38025636359000714 1103 9 0.47842238025165784 1103 10-11 0.4670028261299848 1103 12-13 0.4205235493780535 1103 14-15 0.5655742903037932 1103 16-17 0.5783925876877305 1103 18-19 0.5426031410979775 1103 20-21 0.7250489581307917 1103 22-23 0.7038933354932624 1103 24-25 0.6585990379715199 1103 26-27 0.5447879773685287 1103 28-29 0.5796756799660869 1103 30-31 0.5536287004144071 1103 32-33 0.6215642494855018 1103 34-35 0.6598897391562275 1103 36-37 0.5197635550550856 1103 38-39 0.8776528254187781 1103 40-41 0.8967486882249673 1103 42-43 0.7128782476399778 1103 44-45 0.5977356011354047 1103 46-47 0.5857917749037043 1103 48-49 0.5993784751133546 1103 50-51 0.6229961480794444 1103 52-53 0.7259355751924303 1103 54-55 0.6882895907120385 1103 56-57 0.5443195086798838 1103 58-59 0.5310924959888084 1103 60-61 0.5777612814763486 1103 62-63 0.42594997111603305 1103 64-65 0.3707069722150038 1103 66-67 0.42199541928672346 1103 68-69 0.36779013305841346 1103 70-71 0.3419107091512288 1103 72-73 0.2724596601401963 1103 74-75 0.2693147167970764 1103 76-77 0.23561416241917854 1103 78-79 0.3314706117474735 1103 80-81 0.30024638476129084 1103 82-83 0.20897912894692183 1103 84-85 0.25359820211360784 1103 86-87 0.3132592327032242 1103 88-89 0.23542969504428513 1103 90-91 0.301422318665157 1103 92-93 0.32475302336026246 1103 94-95 0.17896842289699677 1103 96-97 0.3822622007302847 1103 98-99 0.3405378826270109 1103 100-101 0.02088135450068762 1104 1 0.30109004096083325 1104 2 0.40115524138928293 1104 3 0.34961809641865216 1104 4 0.2213440749523201 1104 5 0.21490701962066794 1104 6 0.2930283531834661 1104 7 0.3791759760848876 1104 8 0.4930775425477165 1104 9 0.4004366171821019 1104 10-11 0.495094692069145 1104 12-13 0.5225058908070537 1104 14-15 0.4681674235058395 1104 16-17 0.4653165402181543 1104 18-19 0.5168483393813759 1104 20-21 0.5765226398788244 1104 22-23 0.6665230860360865 1104 24-25 0.6058194013499971 1104 26-27 0.6390274565010188 1104 28-29 0.6571947997216583 1104 30-31 0.6008932656265813 1104 32-33 0.6668058728378625 1104 34-35 0.6642494374263848 1104 36-37 0.5480722746978728 1104 38-39 1.1253899439394033 1104 40-41 0.9258734180427979 1104 42-43 0.8470959151990982 1104 44-45 0.6936892864786799 1104 46-47 0.5810046793766475 1104 48-49 0.7260518192529446 1104 50-51 0.5789092822418596 1104 52-53 0.5354307508516243 1104 54-55 0.7536679811866094 1104 56-57 0.6961211027777665 1104 58-59 0.6845131823238475 1104 60-61 0.7783112495562179 1104 62-63 0.7987356470131672 1104 64-65 0.6724748300944157 1104 66-67 0.6804303973427963 1104 68-69 0.6491014069269383 1104 70-71 0.7986952596869159 1104 72-73 0.6526953150137551 1104 74-75 0.7758270997745669 1104 76-77 0.751462181986188 1104 78-79 0.7112520897016559 1104 80-81 0.6595003279896368 1104 82-83 0.7020756705535902 1104 84-85 0.8645651203038938 1104 86-87 0.8649618343936112 1104 88-89 0.6530219017315666 1104 90-91 0.6379817291924574 1104 92-93 0.7612175046012659 1104 94-95 0.859628643155105 1104 96-97 0.9541455866892434 1104 98-99 0.9868382568650986 1104 100-101 0.6854149851395768 1105 1 0.1483967690189978 1105 2 0.23269895556509113 1105 3 0.2398176138744006 1105 4 0.23001100593257462 1105 5 0.12928651399565183 1105 6 0.13507455303695792 1105 7 0.06987395555980669 1105 8 0.18751148159150688 1105 9 0.22870864008011438 1105 10-11 0.24022289644152295 1105 12-13 0.21789887549209652 1105 14-15 0.43636047657833643 1105 16-17 0.2657410140842771 1105 18-19 0.17370248691847223 1105 20-21 0.2533391448376463 1105 22-23 0.35291206622288485 1105 24-25 0.29201383101482037 1105 26-27 0.1998015022822912 1105 28-29 0.26170630841674125 1105 30-31 0.24315612401591125 1105 32-33 0.3027449549426038 1105 34-35 0.14459187582240673 1105 36-37 0.18155559862301374 1105 38-39 0.6959008011850116 1105 40-41 0.4339938389204221 1105 42-43 0.21515646757519136 1105 44-45 0.20945288990807853 1105 46-47 0.16637654353912268 1105 48-49 0.10760150259250167 1105 50-51 -0.04293653057401059 1105 52-53 -0.02243933361572914 1105 54-55 0.17384390202223443 1105 56-57 0.1830316847049076 1105 58-59 0.2499763378448847 1105 60-61 0.2997489484362248 1105 62-63 0.08440596936275568 1105 64-65 0.08330495653751768 1105 66-67 0.06513238868529214 1105 68-69 -0.04962458192767372 1105 70-71 -0.05097422550587538 1105 72-73 -0.1502839609340505 1105 74-75 -0.1875406581927912 1105 76-77 -0.2169753857196035 1105 78-79 -0.1548324483956094 1105 80-81 -0.1167306093754128 1105 82-83 -0.14767915157270295 1105 84-85 -0.05030045560249974 1105 86-87 -0.19567381522089988 1105 88-89 -0.23354556382046 1105 90-91 -0.12081468105563786 1105 92-93 -0.06394008869087031 1105 94-95 0.032060539418406364 1105 96-97 -0.3223731970162653 1105 98-99 -0.2171758215181292 1105 100-101 -0.19533202991008736 1106 1 0.10545717944835431 1106 2 0.25872261902922133 1106 3 0.24245415239773394 1106 4 0.16321124566869827 1106 5 0.17135824762591056 1106 6 0.1539343535083333 1106 7 0.2683525532431972 1106 8 0.16863240164174442 1106 9 0.19019047755411123 1106 10-11 0.24856015319527813 1106 12-13 0.22722972391487417 1106 14-15 0.3870903724831223 1106 16-17 0.3926242908132451 1106 18-19 0.36591259970341383 1106 20-21 0.420976025641572 1106 22-23 0.34186967512049904 1106 24-25 0.38986975742868424 1106 26-27 0.368000421793262 1106 28-29 0.48831604008272933 1106 30-31 0.4318176951257442 1106 32-33 0.24178422221066143 1106 34-35 0.17189455105958729 1106 36-37 0.20042336235719915 1106 38-39 0.9023942133313696 1106 40-41 0.6598206561160467 1106 42-43 0.5820948165533082 1106 44-45 0.48354713861613874 1106 46-47 0.43322450249897315 1106 48-49 0.3888632466588149 1106 50-51 0.28074397773362847 1106 52-53 0.46207086285333787 1106 54-55 0.41469851379920186 1106 56-57 0.5396517406712817 1106 58-59 0.4545233609118 1106 60-61 0.619069919619001 1106 62-63 0.4777749464467149 1106 64-65 0.585233044083111 1106 66-67 0.5211300527948666 1106 68-69 0.45959603817299666 1106 70-71 0.5078354483216785 1106 72-73 0.3753584438595503 1106 74-75 0.5289357516889197 1106 76-77 0.5759676266569826 1106 78-79 0.7165337541199435 1106 80-81 0.7434653707136363 1106 82-83 0.6901777807269909 1106 84-85 0.7639870349498565 1106 86-87 0.8797278056523439 1106 88-89 0.7790971636948463 1106 90-91 0.8384651046906804 1106 92-93 0.8768246918933009 1106 94-95 0.9740966479255455 1106 96-97 1.249807353901815 1106 98-99 1.059196758592993 1106 100-101 1.1093527436328792 1107 1 0.15330270493864973 1107 2 0.13045027742044368 1107 3 0.21074180853496216 1107 4 0.21493122046398128 1107 5 0.1865532300639643 1107 6 0.13129302392498232 1107 7 0.14447550236425855 1107 8 0.12168979083588738 1107 9 0.31638419317311417 1107 10-11 0.26843494059955475 1107 12-13 0.2668131455691878 1107 14-15 0.32671265975639585 1107 16-17 0.3662289795446654 1107 18-19 0.34694783654802563 1107 20-21 0.32139867608193384 1107 22-23 0.1673741053007589 1107 24-25 0.30427731610695474 1107 26-27 0.343291525542611 1107 28-29 0.3418206998755906 1107 30-31 0.34044372966612 1107 32-33 0.40612549078340976 1107 34-35 0.48960242221637174 1107 36-37 0.3799968727520451 1107 38-39 0.8900761029798687 1107 40-41 0.5813113158599279 1107 42-43 0.3852164946422363 1107 44-45 0.5551241269045804 1107 46-47 0.6019525039925142 1107 48-49 0.5454536417425828 1107 50-51 0.5180771695778574 1107 52-53 0.590537269239725 1107 54-55 0.5044148974744829 1107 56-57 0.3670036043382723 1107 58-59 0.4414304396944644 1107 60-61 0.359445873386818 1107 62-63 0.1997069784839951 1107 64-65 0.33201642697713396 1107 66-67 0.31220615383599437 1107 68-69 0.2550855988308811 1107 70-71 0.09260331182646553 1107 72-73 0.2929504291159013 1107 74-75 0.312484403886657 1107 76-77 0.18145789563813608 1107 78-79 0.38008322786734894 1107 80-81 0.3701654453114003 1107 82-83 0.3072139823658233 1107 84-85 0.40159647287032385 1107 86-87 0.469882217492561 1107 88-89 0.4896573157447328 1107 90-91 0.32962607286590284 1107 92-93 0.38413832716895513 1107 94-95 0.5359108876458443 1107 96-97 0.7675866630154076 1107 98-99 0.9899052319562216 1107 100-101 0.8081832992769407 1108 1 0.007365663653981613 1108 2 0.001860211796422817 1108 3 0.11421754842612586 1108 4 0.02754596644232521 1108 5 0.13205250825283343 1108 6 0.06168343388489461 1108 7 0.0773219154646938 1108 8 0.017790696265862493 1108 9 0.07395726975688177 1108 10-11 0.20475972249530372 1108 12-13 0.18601617811123106 1108 14-15 0.19300166580566014 1108 16-17 0.2475608784400407 1108 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72-73 -1.7104417444401996 2316 74-75 -1.708152593325046 2316 76-77 -1.6560056804107788 2316 78-79 -1.3826574052357223 2316 80-81 -1.3937293473653547 2316 82-83 -1.4357516515780766 2316 84-85 -1.2877256110963273 2316 86-87 -1.166707244512864 2316 88-89 -1.3754125862291993 2316 90-91 -1.324313265325447 2316 92-93 -1.4919445648492022 2316 94-95 -1.374740770320141 2316 96-97 -1.3712229834205267 2316 98-99 -1.4750591920094536 2316 100-101 -0.6840229578422168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6939.0 3 1395.0 4 1441.0 5 1752.0 6 2005.0 7 2482.0 8 3199.0 9 3968.0 10 4468.0 11 5991.0 12 8213.0 13 11111.0 14 11535.0 15 12277.0 16 16238.0 17 20567.0 18 17935.0 19 12425.0 20 12135.0 21 13127.0 22 14248.0 23 15696.0 24 17720.0 25 20349.0 26 23443.0 27 27500.0 28 31941.0 29 38057.0 30 45700.0 31 55561.0 32 69308.0 33 89172.0 34 121790.0 35 178452.0 36 267108.0 37 399909.0 38 350664.0 39 45807.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14012107691856 18.975886846423005 14.116817666986853 21.76717440967158 2 14.933188548225978 21.92144599070625 39.187676684340154 23.957688776727615 3 20.923177407849845 25.796128917452734 29.655028074877798 23.625665599819627 4 12.305234123563038 16.154715464214654 38.29943444262133 33.240615969600974 5 12.824950099093819 39.10359918661649 33.590604722620746 14.48084599166894 6 30.394137943228372 36.41790139984138 17.5844527180969 15.603507938833348 7 27.90644564278832 33.979920301655255 20.835857484767935 17.27777657078849 8 26.215213648695563 32.752392276195785 20.243378762620814 20.78901531248784 9 26.750743594174953 17.858792401038556 21.498992875847314 33.89147112893917 10-11 25.217177553292057 26.278926059335845 26.927616577090767 21.576279810281328 12-13 27.299675904524456 24.2866741943714 27.18842754331588 21.22522235778827 14-15 23.300692473990857 25.488697052924277 27.613565709235704 23.597044763849166 16-17 21.253705104614347 29.04213494852951 27.71452350491846 21.98963644193768 18-19 21.033166607166724 28.306510354884946 29.965838695276158 20.69448434267217 20-21 22.526755046921632 26.299768228648716 29.160606603575463 22.012870120854192 22-23 21.667735309779715 26.446907813546083 29.662845414560657 22.222511462113548 24-25 22.522853888308305 26.57608533533951 29.629438629327904 21.27162214702428 26-27 22.04208768803318 25.966517791135658 30.905518925710258 21.0858755951209 28-29 20.848126018385855 27.830660898456745 30.425698239587284 20.89551484357012 30-31 21.271442640077005 26.609507352177474 29.885469938164732 22.233580069580785 32-33 21.39911361552467 26.98107132422889 29.96662916111918 21.653185899127266 34-35 22.561663564161098 26.490645895071168 29.567190345299753 21.380500195467977 36-37 22.381843983713004 25.847267070951663 30.414612666683905 21.356276278651432 38-39 21.600725782642797 26.93434125215245 30.291440757104827 21.17349220809993 40-41 21.393965685002595 27.239860372340424 30.03900168655942 21.32717225609756 42-43 21.140827500138133 27.382840597517895 28.817809151742154 22.658522750601822 44-45 21.514922017611493 26.6359522458938 28.96161026902872 22.88751546746598 46-47 22.549955706172874 27.53266273704168 28.56395483677186 21.353426720013584 48-49 22.289408141148733 27.240869251097973 28.62804595952874 21.84167664822455 50-51 20.940800421794936 27.590483758740397 29.690510501147642 21.778205318317028 52-53 21.96564292388523 28.39211864918072 27.853232833249596 21.789005593684454 54-55 20.91012754701812 27.324863317134916 28.616478690500724 23.148530445346243 56-57 22.04869701546272 27.2952439297214 28.180757070537783 22.475301984278097 58-59 23.072359234496695 26.80518090821945 28.006802424674778 22.11565743260908 60-61 21.907263156322156 27.333696101625087 29.53593089947714 21.22310984257561 62-63 21.539501638449973 27.34957934156289 29.279089616508656 21.83182940347848 64-65 21.0874700536607 28.279002163005934 28.647192262152366 21.986335521181008 66-67 21.517274702809807 27.423381564523964 27.895316446839384 23.164027285826844 68-69 20.933758537336093 27.505548729686986 28.62404180778298 22.936650925193945 70-71 22.089058590617622 27.431519377797137 28.144624949010467 22.334797082574774 72-73 21.848395669258903 26.679196466981637 28.995654707839247 22.476753155920218 74-75 20.876229136028314 28.84184945313001 28.011587665884402 22.27033374495727 76-77 21.364641081420572 27.165638020447208 28.130489672097948 23.339231226034265 78-79 21.442174670990692 27.394309141911087 28.489034374696686 22.67448181240153 80-81 22.06129617479858 27.55210879347531 28.088357193485546 22.29823783824056 82-83 21.711066550150342 26.963703880926264 28.783223879628633 22.542005689294758 84-85 21.18481023181147 27.46500701925219 28.479864922164744 22.870317826771597 86-87 21.331629112471987 27.505019645175338 28.329047435836998 22.83430380651568 88-89 21.148860603946822 27.331843270689195 27.794349049793578 23.72494707557041 90-91 21.28811190867694 26.557142067617157 28.340510411707232 23.814235611998676 92-93 21.94438599204428 27.046621352675864 28.261960839990568 22.74703181528929 94-95 21.447200060643105 26.520888279528208 28.465346597407105 23.56656506242158 96-97 20.53949603735265 26.550104843275086 29.24099350506622 23.669405614306044 98-99 21.260618622457063 27.189453539430964 27.72856989664106 23.821357941470918 100-101 20.900425294194136 25.81476700321322 28.853748629054195 24.431059073538457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3922.0 1 2438.5 2 1226.5 3 1477.5 4 1246.0 5 1221.5 6 2162.0 7 4142.0 8 6064.5 9 6364.0 10 5175.0 11 4253.0 12 4130.5 13 4212.0 14 4345.0 15 4372.0 16 4360.0 17 4353.5 18 4353.5 19 4479.5 20 4651.0 21 5026.5 22 5696.5 23 6451.5 24 7222.0 25 8338.5 26 10211.5 27 12441.5 28 13997.5 29 16156.0 30 19974.0 31 23912.0 32 28170.5 33 32725.0 34 37165.0 35 40927.5 36 46014.5 37 50475.5 38 52951.0 39 54411.0 40 54585.0 41 57827.0 42 60807.0 43 63273.0 44 66240.5 45 68809.0 46 70745.5 47 84092.5 48 121053.5 49 119536.0 50 82449.5 51 65469.0 52 58568.0 53 56987.0 54 56300.5 55 52030.0 56 45120.5 57 40058.5 58 36883.0 59 32445.0 60 27751.0 61 23859.5 62 20357.0 63 15829.5 64 11651.5 65 9154.5 66 6919.0 67 5822.5 68 4645.5 69 2994.0 70 2227.5 71 1793.5 72 1461.0 73 1210.0 74 967.5 75 806.0 76 575.0 77 344.0 78 214.0 79 136.0 80 102.5 81 73.0 82 51.5 83 41.5 84 26.5 85 20.0 86 13.5 87 9.5 88 6.0 89 5.5 90 7.0 91 4.5 92 4.0 93 4.0 94 4.0 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28754077447275783 2 0.17803507412072472 3 0.17808553757710816 4 0.1986746277815457 5 0.2126025417433711 6 0.10708345444562865 7 0.12207110099150599 8 0.1341823305235281 9 0.1377652359267513 10-11 0.1257044698511126 12-13 0.1149052901850596 14-15 0.1296153877208281 16-17 0.10594802667700158 18-19 0.13314782966766786 20-21 0.16014577883280043 22-23 0.16483888027645902 24-25 0.18878379033039433 26-27 0.1694310548073507 28-29 0.1674125165520137 30-31 0.15484711591254077 32-33 0.16440994089719987 34-35 0.3473904337435003 36-37 0.46734206956690244 38-39 0.4334558586054323 40-41 0.42308561831863833 42-43 0.44766132157736654 44-45 0.4512946904369731 46-47 0.4546505102864709 48-49 0.4665094225365759 50-51 0.45969685592481346 52-53 0.4657272389626328 54-55 0.449553701191745 56-57 0.41094915705842455 58-59 0.3998724283822627 60-61 0.3533198898685528 62-63 0.46368346897910406 64-65 0.4615135403546168 66-67 0.4706221942318251 68-69 0.48238017956916324 70-71 0.4750377466653748 72-73 0.4676700820333947 74-75 0.47246411038982006 76-77 0.4487967493459936 78-79 0.4352725430352356 80-81 0.43259797984691406 82-83 0.44428026999967707 84-85 0.44627357652682237 86-87 0.44826688305396767 88-89 0.45457481510189585 90-91 0.47339768433291346 92-93 0.4777375415818881 94-95 0.476248869618577 96-97 0.47448264864515716 98-99 0.47084927978555047 100-101 0.4540449488098699 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1981632.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.44854068402131 #Duplication Level Percentage of deduplicated Percentage of total 1 69.51692300856234 25.33790396505562 2 13.971933983902307 10.185132084934464 3 5.899307953029687 6.45063497800719 4 3.022032443944392 4.405946899261582 5 1.815624013033902 3.308842285297611 6 1.202172961918285 2.629047006702541 7 0.7973474369521197 2.0343505346534623 8 0.569706715494973 1.6611982718142941 9 0.43241356293065025 1.4184759006720373 >10 2.363191527494591 16.36059361773498 >50 0.2523564019189617 6.423756226014545 >100 0.14571714845563188 9.707902600012766 >500 0.005288493947641198 1.3018258965882623 >1k 0.004453468587487325 3.48080162967139 >5k 0.0011133671468718312 2.8975061190390483 >10k+ 4.1751268007693664E-4 2.3960819845403347 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23021 1.1617192294028356 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12243 0.6178240965022769 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11972 0.6041484998223686 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9949 0.5020609275586991 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9160 0.4622452604721765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8603 0.4341371152666086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6707 0.338458401963634 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6514 0.3287189548816329 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5938 0.2996520040047799 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5227 0.2637724865161645 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5023 0.2534779414139457 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4594 0.2318291186254562 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4517 0.22794343248393242 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3913 0.19746350482834352 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3308 0.16693311371637115 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3160 0.15946452217162418 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2851 0.14387131414914575 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2814 0.14200416626295903 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2727 0.137613845557601 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2706 0.13655411297354905 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2621 0.13226471918095792 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2536 0.12797532538836676 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2526 0.12747069082453252 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.11510714401059328 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2069 0.1044088912573071 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1998 0.1008259858540839 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1985 0.10016996092109937 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5231728191712688E-5 0.0 12-13 0.0 0.0 0.0 7.569518457513806E-5 0.0 14-15 0.0 0.0 0.0 1.0092691276685075E-4 0.0 16-17 0.0 0.0 0.0 1.5139036915027613E-4 0.0 18-19 0.0 0.0 0.0 1.5139036915027613E-4 0.0 20-21 0.0 0.0 0.0 1.5139036915027613E-4 0.0 22-23 0.0 0.0 0.0 4.2893937925911574E-4 0.0 24-25 0.0 0.0 0.0 0.0011606594968187837 0.0 26-27 0.0 0.0 0.0 0.002094233439912153 0.0 28-29 0.0 0.0 0.0 0.005525748473985079 0.0 30-31 0.0 0.0 0.0 0.015164268643219327 0.0 32-33 0.0 0.0 0.0 0.0351982608274392 0.0 34-35 0.0 0.0 0.0 0.06736871427187288 2.5231728191712688E-5 36-37 0.0 0.0 0.0 0.11546038820527726 5.0463456383425376E-5 38-39 0.0 0.0 0.0 0.18938935180699545 5.0463456383425376E-5 40-41 0.0 0.0 0.0 0.2722251154603882 5.0463456383425376E-5 42-43 0.0 0.0 0.0 0.35763451538933566 5.0463456383425376E-5 44-45 0.0 0.0 0.0 0.4617658576365339 5.0463456383425376E-5 46-47 0.0 0.0 0.0 0.594535211381326 5.0463456383425376E-5 48-49 0.0 0.0 0.0 0.7295501889351808 5.0463456383425376E-5 50-51 0.0 0.0 0.0 0.8803349966088557 5.0463456383425376E-5 52-53 0.0 0.0 0.0 1.0289246439298518 5.0463456383425376E-5 54-55 0.0 0.0 0.0 1.1935364386525853 5.0463456383425376E-5 56-57 0.0 0.0 0.0 1.4076024206310758 5.0463456383425376E-5 58-59 5.0463456383425376E-5 0.0 0.0 1.6289099086005878 5.0463456383425376E-5 60-61 5.0463456383425376E-5 0.0 0.0 1.8812776539740983 5.0463456383425376E-5 62-63 5.0463456383425376E-5 0.0 0.0 2.1338472531731423 5.0463456383425376E-5 64-65 5.0463456383425376E-5 0.0 0.0 2.386341157187611 5.0463456383425376E-5 66-67 5.0463456383425376E-5 0.0 0.0 2.6925281787940447 5.0463456383425376E-5 68-69 5.0463456383425376E-5 0.0 0.0 3.0404989422859545 5.0463456383425376E-5 70-71 5.0463456383425376E-5 0.0 0.0 3.415947057778639 5.0463456383425376E-5 72-73 5.0463456383425376E-5 0.0 0.0 3.7985609840777705 5.0463456383425376E-5 74-75 5.0463456383425376E-5 0.0 0.0 4.20252095242709 5.0463456383425376E-5 76-77 5.0463456383425376E-5 0.0 0.0 4.613747658495624 5.0463456383425376E-5 78-79 5.0463456383425376E-5 0.0 0.0 5.123403336240028 5.0463456383425376E-5 80-81 5.0463456383425376E-5 0.0 0.0 5.685238227884895 5.0463456383425376E-5 82-83 5.0463456383425376E-5 0.0 0.0 6.220024706908245 5.0463456383425376E-5 84-85 5.0463456383425376E-5 0.0 0.0 6.780623243871718 5.0463456383425376E-5 86-87 5.0463456383425376E-5 0.0 0.0 7.36302199399283 5.0463456383425376E-5 88-89 5.0463456383425376E-5 0.0 0.0 7.995682346671834 5.0463456383425376E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12290 0.0 27.719084 1 TACATGG 12625 0.0 26.736427 2 ACATGGG 12715 0.0 25.42862 3 CATGGGG 4445 0.0 24.956743 4 GAGTACT 8545 0.0 23.31376 12-13 AGTACTT 8920 0.0 22.094637 12-13 GTACTTT 9285 0.0 21.486631 14-15 CATGGGA 7985 0.0 21.195179 4 ATGGGGG 2210 0.0 20.59315 5 TACCTGG 1190 0.0 19.919474 2 GCGTAAA 120 0.0047957147 19.769472 1 AGTACAT 9205 0.0 18.64402 2 TACTTTT 10810 0.0 18.214342 14-15 ACTTTTT 11270 0.0 17.981153 16-17 GGACAGT 825 0.0 17.802792 6 GTGTAAG 670 0.0 17.704004 1 ATGGGGA 2465 0.0 17.693539 5 GGTTCGC 135 0.009542525 17.547554 8 ATGGGAT 3340 0.0 17.316383 5 TACCCGG 165 0.0014833821 17.239399 2 >>END_MODULE