##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2017183 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86628382253866 33.0 31.0 34.0 30.0 34.0 2 31.90621574740616 33.0 31.0 34.0 30.0 34.0 3 31.96372565106884 34.0 31.0 34.0 30.0 34.0 4 35.464573615780026 37.0 35.0 37.0 33.0 37.0 5 35.527424631280354 37.0 35.0 37.0 33.0 37.0 6 35.6470578028865 37.0 35.0 37.0 33.0 37.0 7 35.57258563055509 37.0 35.0 37.0 33.0 37.0 8 35.5617928566719 37.0 35.0 37.0 33.0 37.0 9 37.16137603777149 39.0 37.0 39.0 33.0 39.0 10-11 37.07623577037879 39.0 37.0 39.0 33.0 39.0 12-13 36.98164569104539 39.0 37.0 39.0 33.0 39.0 14-15 38.15035522310073 40.0 38.0 41.0 33.0 41.0 16-17 38.12674333464044 40.0 38.0 41.0 33.0 41.0 18-19 38.116603203576474 40.0 38.0 41.0 33.0 41.0 20-21 38.02211202454116 40.0 38.0 41.0 33.0 41.0 22-23 37.94532920414261 40.0 38.0 41.0 33.0 41.0 24-25 37.94432904699276 40.0 38.0 41.0 33.0 41.0 26-27 37.84292996718692 40.0 38.0 41.0 33.0 41.0 28-29 37.72197688558748 40.0 37.0 41.0 32.0 41.0 30-31 37.54518182039011 40.0 37.0 41.0 32.0 41.0 32-33 37.32517996631937 40.0 36.5 41.0 31.5 41.0 34-35 36.98657137205697 40.0 36.0 41.0 31.0 41.0 36-37 36.715213245402126 39.5 35.5 41.0 30.0 41.0 38-39 36.39461243724541 39.0 35.0 41.0 30.0 41.0 40-41 36.29088238399788 39.0 35.0 41.0 30.0 41.0 42-43 36.09957921517284 39.0 35.0 41.0 29.5 41.0 44-45 36.00634002963538 39.0 35.0 41.0 29.0 41.0 46-47 35.748726069969855 39.0 35.0 41.0 27.5 41.0 48-49 35.49491295534416 39.0 35.0 41.0 26.5 41.0 50-51 34.308522330398375 38.0 34.0 40.0 24.5 40.5 52-53 34.45575165961641 38.0 34.0 39.5 24.5 40.5 54-55 34.97603514405981 39.0 35.0 40.5 26.0 41.0 56-57 34.86383585425814 38.5 35.0 41.0 24.5 41.0 58-59 34.66241486270705 38.0 34.5 40.0 24.0 41.0 60-61 34.33795148977559 38.0 34.0 40.0 23.0 41.0 62-63 34.009174675773096 37.5 34.0 40.0 22.5 41.0 64-65 33.63280946746032 37.0 33.5 40.0 22.0 41.0 66-67 33.233506578233104 36.5 33.0 39.0 21.0 41.0 68-69 32.808018161961506 36.0 33.0 39.0 20.0 41.0 70-71 32.34415048114127 35.0 32.0 38.5 18.0 40.0 72-73 31.897344217158285 35.0 32.0 37.5 16.0 40.0 74-75 31.458426925073233 35.0 31.0 37.0 12.0 39.0 76-77 31.021502015434397 35.0 31.0 36.5 7.5 39.0 78-79 30.585760191316307 35.0 31.0 36.0 6.0 38.5 80-81 30.093171021171607 34.5 30.0 35.5 2.0 37.0 82-83 29.641005302939796 34.0 30.0 35.0 2.0 37.0 84-85 29.215727576526277 34.0 29.0 35.0 2.0 36.5 86-87 28.932695744511033 34.0 29.0 35.0 2.0 36.0 88-89 28.510064530585474 34.0 29.0 35.0 2.0 36.0 90-91 28.080610683314305 34.0 28.5 35.0 2.0 35.0 92-93 27.654302559559543 34.0 27.0 35.0 2.0 35.0 94-95 27.153499707265034 34.0 26.0 35.0 2.0 35.0 96-97 26.566111503021787 33.0 25.0 35.0 2.0 35.0 98-99 25.859056168924685 33.0 23.5 35.0 2.0 35.0 100-101 23.445679445047872 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3066698234300169 1101 2 0.14053449792101347 1101 3 0.3380719229424649 1101 4 0.3748969901389998 1101 5 0.31547371446644945 1101 6 0.3245929778129195 1101 7 0.4020388137600861 1101 8 0.4929518763960914 1101 9 0.510710071623862 1101 10-11 0.4917947133583169 1101 12-13 0.5050194150851155 1101 14-15 0.5517449031075472 1101 16-17 0.603471747996835 1101 18-19 0.593720784587596 1101 20-21 0.6324339574303863 1101 22-23 0.6045037946257423 1101 24-25 0.6566372509292222 1101 26-27 0.47589804721743434 1101 28-29 0.5521374774875696 1101 30-31 0.6919924197108074 1101 32-33 0.7121985294219826 1101 34-35 0.6800329688997948 1101 36-37 0.6359698434651762 1101 38-39 0.7847305024641216 1101 40-41 0.6269604193122404 1101 42-43 0.5490261965744381 1101 44-45 0.622540626711718 1101 46-47 0.5568220799054018 1101 48-49 0.6418829443847613 1101 50-51 0.5739226296953532 1101 52-53 0.6430767437102602 1101 54-55 0.7541342210636586 1101 56-57 0.7043652676458194 1101 58-59 0.6765454026493813 1101 60-61 0.8019444263276654 1101 62-63 0.8198227686114876 1101 64-65 0.7109830910139436 1101 66-67 0.6168715168443057 1101 68-69 0.5781082633754338 1101 70-71 0.46775051672586443 1101 72-73 0.6369091189058871 1101 74-75 0.49260209531397336 1101 76-77 0.5443022635779364 1101 78-79 0.4871606096262937 1101 80-81 0.45726518726761967 1101 82-83 0.4666177535154077 1101 84-85 0.43969258572538195 1101 86-87 0.1866459123630939 1101 88-89 0.3364545593671977 1101 90-91 0.4943345817916516 1101 92-93 0.5242940826914015 1101 94-95 0.2575360621293683 1101 96-97 0.20573036193239247 1101 98-99 0.21463684122186777 1101 100-101 -0.0042910386921164445 1102 1 0.358111627724913 1102 2 0.43624120364391317 1102 3 0.5299529813148176 1102 4 0.3075513361077924 1102 5 0.4333917220849841 1102 6 0.43961035397962434 1102 7 0.4960910900058053 1102 8 0.4277485334914104 1102 9 0.5471300031469895 1102 10-11 0.5041931382617975 1102 12-13 0.6050044799325889 1102 14-15 0.5167555343666592 1102 16-17 0.537025575571306 1102 18-19 0.5604308113745162 1102 20-21 0.5229729397844238 1102 22-23 0.5557225351909025 1102 24-25 0.5803507401020411 1102 26-27 0.5162004760265333 1102 28-29 0.6108759870557066 1102 30-31 0.5963644045765193 1102 32-33 0.5104443716682283 1102 34-35 0.4552579597459925 1102 36-37 0.5346118687360928 1102 38-39 0.5287916113269304 1102 40-41 0.4181968572301855 1102 42-43 0.4826248296065003 1102 44-45 0.6108202256145461 1102 46-47 0.3682302881367576 1102 48-49 0.30323831750661867 1102 50-51 0.17989685130090294 1102 52-53 0.27046122913804993 1102 54-55 0.24263078116077708 1102 56-57 0.3059806730147656 1102 58-59 0.17461282603044737 1102 60-61 0.10295760780422114 1102 62-63 0.20854303481051062 1102 64-65 0.13680532736835005 1102 66-67 0.3146412007329715 1102 68-69 0.3832260423094951 1102 70-71 0.21342333731774943 1102 72-73 0.12522854488213042 1102 74-75 -0.1014660796425062 1102 76-77 0.044787093959566704 1102 78-79 0.05421221780497376 1102 80-81 -0.06121955318191041 1102 82-83 -0.3292288287435561 1102 84-85 -0.10915178677337778 1102 86-87 -0.3038163065187902 1102 88-89 -0.28190074309372903 1102 90-91 -0.3539038731748505 1102 92-93 -0.32966501747369037 1102 94-95 -0.23197078876962962 1102 96-97 -0.2995623028200036 1102 98-99 -0.41688596620138796 1102 100-101 -0.6872985287514055 1103 1 0.34551845693826877 1103 2 0.4411141628810533 1103 3 0.4023972665531268 1103 4 0.3248369513986731 1103 5 0.23865887293992216 1103 6 0.307622486321641 1103 7 0.37614515057052955 1103 8 0.43610852429360847 1103 9 0.44346292612476645 1103 10-11 0.4509395713751445 1103 12-13 0.5010222060413696 1103 14-15 0.6210198429749028 1103 16-17 0.5866069034026395 1103 18-19 0.6163217078945209 1103 20-21 0.6187184859666317 1103 22-23 0.5061330732468221 1103 24-25 0.5680963863963981 1103 26-27 0.4652895336113616 1103 28-29 0.44482276805879906 1103 30-31 0.5595672427561666 1103 32-33 0.5125761349092315 1103 34-35 0.41928703607055695 1103 36-37 0.49038685414944183 1103 38-39 0.6613673250562186 1103 40-41 0.6719735101366453 1103 42-43 0.4226731337222063 1103 44-45 0.508644506879655 1103 46-47 0.6144520709778476 1103 48-49 0.6458779622336053 1103 50-51 0.6151106767897758 1103 52-53 0.6114257159146419 1103 54-55 0.7468079609074678 1103 56-57 0.7096142750345322 1103 58-59 0.6656028883346181 1103 60-61 0.655091013510301 1103 62-63 0.6562610251215304 1103 64-65 0.5393009357295924 1103 66-67 0.5276855019266478 1103 68-69 0.5121476623118966 1103 70-71 0.33593871782998264 1103 72-73 0.4709996665919185 1103 74-75 0.31208003373764015 1103 76-77 0.45441772702817573 1103 78-79 0.5422495971815593 1103 80-81 0.23782750422458676 1103 82-83 0.18227030387845744 1103 84-85 0.35830941585519227 1103 86-87 0.35158607396058983 1103 88-89 0.40428221500151196 1103 90-91 0.5019982859628129 1103 92-93 0.4974154773932149 1103 94-95 0.28788348547197984 1103 96-97 0.2904608950438501 1103 98-99 0.19670504122412424 1103 100-101 -0.0851228100165109 1104 1 0.22164931459482418 1104 2 0.35252900516264063 1104 3 0.37955305248144455 1104 4 0.3917883884597373 1104 5 0.388239565076681 1104 6 0.32051637875603944 1104 7 0.33717049140858535 1104 8 0.45561364205885724 1104 9 0.555289318229562 1104 10-11 0.4546031899342893 1104 12-13 0.42830694883170395 1104 14-15 0.4484598063581444 1104 16-17 0.5198052458428322 1104 18-19 0.4257107002073397 1104 20-21 0.41939022161806605 1104 22-23 0.5053101828731528 1104 24-25 0.46411293927349817 1104 26-27 0.5078982141855022 1104 28-29 0.4207882404457095 1104 30-31 0.49408983330176426 1104 32-33 0.5514105813244967 1104 34-35 0.5731488508779208 1104 36-37 0.6668650746852549 1104 38-39 0.7366699409357764 1104 40-41 0.5453047467562655 1104 42-43 0.6957678024389438 1104 44-45 0.6969964151631771 1104 46-47 0.6489961269290063 1104 48-49 0.6577223517954351 1104 50-51 0.6473896541156634 1104 52-53 0.687576035937532 1104 54-55 0.7773088672079851 1104 56-57 0.6909103729646162 1104 58-59 0.657263966920425 1104 60-61 0.6922671228128578 1104 62-63 0.8168857880080225 1104 64-65 0.8086162879490928 1104 66-67 0.747770185642274 1104 68-69 0.7758500202478373 1104 70-71 0.7719410162427707 1104 72-73 0.5377506502486149 1104 74-75 0.6209089224664233 1104 76-77 0.7191941026452717 1104 78-79 0.76827882301885 1104 80-81 0.6998628850726014 1104 82-83 0.6853264335223308 1104 84-85 0.6573664627116607 1104 86-87 0.635881020591551 1104 88-89 0.748224979500602 1104 90-91 0.7825357402553976 1104 92-93 0.8021834282870621 1104 94-95 0.774966423553856 1104 96-97 0.9884326234903256 1104 98-99 0.7604226162790475 1104 100-101 0.5876514322143152 1105 1 0.31940312187759545 1105 2 0.33584018429813156 1105 3 0.3767296937430906 1105 4 0.29988172762373466 1105 5 0.35208194750325106 1105 6 0.33774054453191127 1105 7 0.416058524208367 1105 8 0.34494908553615744 1105 9 0.4142182697749206 1105 10-11 0.4396514201435906 1105 12-13 0.4663725460811108 1105 14-15 0.5022398446739231 1105 16-17 0.5201547673584344 1105 18-19 0.47447148230258307 1105 20-21 0.5702228550407185 1105 22-23 0.6089462767467992 1105 24-25 0.6062982050544647 1105 26-27 0.6513515619566661 1105 28-29 0.603811993447799 1105 30-31 0.6884602376072024 1105 32-33 0.45344983763757796 1105 34-35 0.48232343636883 1105 36-37 0.6488666688619986 1105 38-39 0.8223526025862213 1105 40-41 0.7719870196959846 1105 42-43 0.5848549511531971 1105 44-45 0.7327741431952361 1105 46-47 0.7772455056859684 1105 48-49 0.8379626583216151 1105 50-51 0.7236588062172515 1105 52-53 0.8201764297385168 1105 54-55 0.7384901429195807 1105 56-57 0.8119660002465565 1105 58-59 0.7783681830435896 1105 60-61 0.8113635786039595 1105 62-63 0.796776370565091 1105 64-65 0.7941747009912632 1105 66-67 0.9848507598235514 1105 68-69 0.8393534665849316 1105 70-71 0.7603002289184317 1105 72-73 0.8513583601408392 1105 74-75 0.7942711603758958 1105 76-77 0.969472331605143 1105 78-79 0.8685054552568516 1105 80-81 0.8263564135017027 1105 82-83 0.6881586134491222 1105 84-85 0.5394712792540766 1105 86-87 0.5333830046359047 1105 88-89 0.7478393055733754 1105 90-91 0.6632906257476954 1105 92-93 0.8559267357526217 1105 94-95 0.8628801228305747 1105 96-97 0.5858651081385666 1105 98-99 0.6367107990101104 1105 100-101 0.7099783228986745 1106 1 0.20811413337736795 1106 2 0.24393149320890117 1106 3 0.22406086586614649 1106 4 0.286612630262546 1106 5 0.1920578895863727 1106 6 0.20070936262560224 1106 7 0.3127351075394742 1106 8 0.2638903593161359 1106 9 0.3007341631196354 1106 10-11 0.37969729027531685 1106 12-13 0.4219471122826235 1106 14-15 0.4815132377740525 1106 16-17 0.5785788737468991 1106 18-19 0.5057526108456756 1106 20-21 0.49227059066155476 1106 22-23 0.4850293832864594 1106 24-25 0.5499674931891505 1106 26-27 0.4694663729174664 1106 28-29 0.5164497372510013 1106 30-31 0.30890991164687875 1106 32-33 0.30315483910506913 1106 34-35 0.44303671358184715 1106 36-37 0.3627136453910751 1106 38-39 0.5760007566636176 1106 40-41 0.6504283913085303 1106 42-43 0.42969778536257763 1106 44-45 0.35590782027662016 1106 46-47 0.40016600783383893 1106 48-49 0.40732149927254824 1106 50-51 0.40276299345098465 1106 52-53 0.31540320117497345 1106 54-55 0.42897070092094935 1106 56-57 0.3560240060631443 1106 58-59 0.4140908544062185 1106 60-61 0.44973106439600485 1106 62-63 0.5200936504640552 1106 64-65 0.3311948767341235 1106 66-67 0.3603843919908982 1106 68-69 0.324827832229694 1106 70-71 0.3687804222711577 1106 72-73 0.5396866017339086 1106 74-75 0.3368830786056627 1106 76-77 0.41537648237401825 1106 78-79 0.49688067871532127 1106 80-81 0.516661873448161 1106 82-83 0.5835603161804315 1106 84-85 0.44503589543914046 1106 86-87 0.5549086701196835 1106 88-89 0.4289398885665143 1106 90-91 0.4759901819436081 1106 92-93 0.5273771924528212 1106 94-95 0.563844329452948 1106 96-97 0.6084538689557348 1106 98-99 0.4626777849666901 1106 100-101 0.2962819306968214 1107 1 0.20623098328173128 1107 2 0.19024554823739592 1107 3 0.0790182244214499 1107 4 0.22630452798721024 1107 5 0.32057226133255057 1107 6 0.21110423357357178 1107 7 0.2784717700961892 1107 8 0.16251852174966075 1107 9 0.2407849469771861 1107 10-11 0.24532797052353317 1107 12-13 0.34049759711294314 1107 14-15 0.38718858237689346 1107 16-17 0.36141655406512996 1107 18-19 0.36336901367892693 1107 20-21 0.4725154188798868 1107 22-23 0.463721621458582 1107 24-25 0.32065288286252525 1107 26-27 0.44173420191557256 1107 28-29 0.40922268445171284 1107 30-31 0.5117814549962105 1107 32-33 0.41143623683160513 1107 34-35 0.40001032810232573 1107 36-37 0.5578398755046976 1107 38-39 0.5759569314024162 1107 40-41 0.5776092458690556 1107 42-43 0.7227244380692923 1107 44-45 0.47763515553506153 1107 46-47 0.47967478544575215 1107 48-49 0.4798278552715587 1107 50-51 0.4422552991781501 1107 52-53 0.4150602417833511 1107 54-55 0.4644302809359928 1107 56-57 0.3103066426550001 1107 58-59 0.4622605618512239 1107 60-61 0.3028445552544241 1107 62-63 0.3646108430605821 1107 64-65 0.4048471029772287 1107 66-67 0.22376368305850036 1107 68-69 0.3428833053901954 1107 70-71 0.2633810699230068 1107 72-73 0.1508962751448557 1107 74-75 0.3156374471023504 1107 76-77 0.1563196180287001 1107 78-79 0.28702408352124564 1107 80-81 0.36533979358677726 1107 82-83 0.4394134203183384 1107 84-85 0.28612910589071916 1107 86-87 0.2961249119752658 1107 88-89 0.27293585450467006 1107 90-91 0.2869489824568987 1107 92-93 0.3725120807277982 1107 94-95 0.1102503373618049 1107 96-97 0.22858385361154987 1107 98-99 0.308263351918594 1107 100-101 0.13463846736814133 1108 1 0.10145440392888005 1108 2 0.2139169582497793 1108 3 0.06197843469970721 1108 4 0.20473287929691963 1108 5 0.20945107942372942 1108 6 0.13634366678907384 1108 7 0.16402722494376576 1108 8 0.14484427177232106 1108 9 0.04812051602760903 1108 10-11 0.22172453839567652 1108 12-13 0.273275022412804 1108 14-15 0.2616107703584021 1108 16-17 0.2935746253059577 1108 18-19 0.27186322800564255 1108 20-21 0.3716740037313926 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-1.34661861107082 2316 94-95 -1.3163953972468327 2316 96-97 -1.1971436807664482 2316 98-99 -1.3349312222627567 2316 100-101 -0.8236266619214447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5657.0 3 1470.0 4 1480.0 5 1581.0 6 1926.0 7 2373.0 8 2997.0 9 3629.0 10 4612.0 11 6048.0 12 8233.0 13 11998.0 14 11340.0 15 12517.0 16 16182.0 17 21699.0 18 17738.0 19 12477.0 20 12425.0 21 13282.0 22 14191.0 23 15777.0 24 17821.0 25 20469.0 26 23805.0 27 27817.0 28 32196.0 29 38171.0 30 46336.0 31 56205.0 32 70520.0 33 89511.0 34 122036.0 35 178532.0 36 264952.0 37 401273.0 38 371818.0 39 56080.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13405618776318 19.02921743799311 14.131605243926085 21.70512113031763 2 14.901489995151298 21.92843278793314 39.2118405556799 23.95823666123566 3 20.878152745350295 25.837472800622606 29.59510649449659 23.689267959530504 4 12.242125467379365 16.19082032927134 38.37169074562799 33.195363457721314 5 12.840940904569308 39.076290149967335 33.58797799234775 14.494790953115599 6 30.38913356510749 36.43032415479233 17.599624611181408 15.580917668918767 7 27.89153070386483 34.04743361427076 20.780010615763914 17.28102506610049 8 26.21680428143659 32.70965168679791 20.26389889332722 20.809645138438277 9 26.750076643519826 17.838940464107516 21.549826225080935 33.861156667291716 10-11 25.191322307182705 26.331703709178683 26.90896319741337 21.56801078622524 12-13 27.259247647503244 24.30432123232186 27.159859651350775 21.276571468824116 14-15 23.314904931271734 25.48107306377765 27.64617468041728 23.557847324533338 16-17 21.248441457201242 29.018795771924825 27.735402279521537 21.997360491352396 18-19 20.932649504131227 28.343061867824055 30.015767890304897 20.70852073773983 20-21 22.547127385639023 26.316499676172473 29.19468272963663 21.941690208551876 22-23 21.70780570986866 26.413240370060375 29.67845708618386 22.200496833887108 24-25 22.53021380012871 26.59201433769296 29.638302718038766 21.239469144139562 26-27 22.023386732037512 25.972356984945964 30.929899594293563 21.074356688722958 28-29 20.803296440226593 27.878859294743386 30.39537188524478 20.92247237978524 30-31 21.28719453657375 26.617706521927875 29.892512595598795 22.202586345899576 32-33 21.389989125516934 27.03254109681465 29.904629989760522 21.67283978790789 34-35 22.57117705418791 26.531057960100053 29.53223791884001 21.36552706687203 36-37 22.336091827634117 25.862221232652644 30.46178761874317 21.339899320970073 38-39 21.528277772099084 26.939383154777847 30.314913729062727 21.21742534406034 40-41 21.363475185240997 27.275521332989182 30.066059632208535 21.294943849561285 42-43 21.18373321295284 27.39847559496939 28.77459374692986 22.64319744514791 44-45 21.513902698152535 26.582326027531515 29.000556461155803 22.903214813160144 46-47 22.52807121590855 27.571163169291026 28.56542255090831 21.335343063892118 48-49 22.289376642336492 27.227494672124823 28.643169447577836 21.839959237960844 50-51 20.948161231940393 27.602977852687093 29.68165336749862 21.767207547873895 52-53 21.944541329800018 28.46701273396984 27.855167201457242 21.7332787347729 54-55 20.914586081418868 27.2821184297488 28.61945717936542 23.18383830946691 56-57 22.06495256668604 27.316649917597562 28.180414456771857 22.43798305894454 58-59 23.05718014776364 26.77999041697116 28.021507360208748 22.14132207505645 60-61 21.875332424664492 27.34936626810503 29.5492594945455 21.226041812684976 62-63 21.50180919890841 27.412024780602923 29.324716631795937 21.76144938869273 64-65 21.056947463119094 28.27502149561728 28.68294064025094 21.985090401012684 66-67 21.50549245218565 27.406329997962303 27.95217905461302 23.13599849523903 68-69 20.92274670101905 27.538773959307157 28.600698576992144 22.937780762681644 70-71 22.111863837411118 27.44691413321389 28.158657285581867 22.282564743793117 72-73 21.81459059654968 26.705772034959473 29.03853488471032 22.44110248378053 74-75 20.85756681873552 28.856308866967616 28.039137825335786 22.246986488961078 76-77 21.348797037146124 27.212165054523634 28.20432706420147 23.23471084412877 78-79 21.4437053645264 27.407396353678966 28.48517632838527 22.663721953409365 80-81 22.040943825970086 27.574209876279156 28.148345638433497 22.236500659317265 82-83 21.674647099061005 26.948796716622102 28.848927305515826 22.52762887880107 84-85 21.12707413760851 27.52797026057815 28.457078436133077 22.887877165680262 86-87 21.30956867163899 27.547357070633577 28.343973205679738 22.799101052047693 88-89 21.113490246828903 27.360027584374524 27.855018836137425 23.671463332659147 90-91 21.271211851638036 26.622116438586254 28.366866275908663 23.739805433867048 92-93 21.917117642565323 27.092618161740205 28.272972773239886 22.71729142245458 94-95 21.419592394509323 26.554821410709334 28.515638413231926 23.509947781549414 96-97 20.549104856662105 26.588267906474904 29.267377446259797 23.595249790603198 98-99 21.261529527358732 27.19442009565735 27.800271550832928 23.743778826150987 100-101 20.877230339381732 25.902359592598636 28.874810448515625 24.345599619504004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3324.0 1 2056.5 2 1090.0 3 1427.0 4 1229.0 5 1159.5 6 2149.5 7 4267.5 8 6341.0 9 6618.0 10 5304.5 11 4209.5 12 4018.5 13 4228.0 14 4375.5 15 4436.5 16 4548.5 17 4551.0 18 4537.5 19 4602.0 20 4703.0 21 5109.5 22 5810.0 23 6607.0 24 7337.5 25 8490.0 26 10432.5 27 12559.5 28 14165.5 29 16298.0 30 20159.0 31 24413.0 32 28708.5 33 33546.0 34 38220.5 35 41810.0 36 46848.5 37 51677.0 38 54293.5 39 55695.0 40 55915.0 41 58847.0 42 61893.0 43 64797.5 44 67618.5 45 69799.0 46 71839.5 47 86095.5 48 123462.5 49 121375.0 50 83931.0 51 66577.5 52 59440.5 53 58005.5 54 57197.5 55 52929.0 56 46073.5 57 40646.0 58 37171.0 59 32722.5 60 28204.5 61 24299.0 62 20638.0 63 16043.5 64 11832.0 65 9242.0 66 7059.5 67 5923.5 68 4602.5 69 2990.0 70 2325.5 71 1832.5 72 1444.5 73 1285.0 74 1023.5 75 800.0 76 575.5 77 332.5 78 202.0 79 137.0 80 91.5 81 64.0 82 49.0 83 35.0 84 27.0 85 20.0 86 14.0 87 11.0 88 7.5 89 6.5 90 5.5 91 4.0 92 4.5 93 5.5 94 4.5 95 1.5 96 0.5 97 2.5 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28321674334951263 2 0.10955872620381987 3 0.12051459882420187 4 0.12353861796376434 5 0.12943793399012385 6 0.05651445605083921 7 0.06509077262697534 8 0.06994903288397732 9 0.06727203233420072 10-11 0.06338046672017363 12-13 0.06672671740739436 14-15 0.06310780925677045 16-17 0.06333089263591851 18-19 0.06828830106143072 20-21 0.07185763512779951 22-23 0.08031001649329783 24-25 0.08903505532219932 26-27 0.08970430545964347 28-29 0.0830613781694571 30-31 0.07921938663968514 32-33 0.07213029259120268 34-35 0.23917016948883665 36-37 0.3634028246321727 38-39 0.334302837174416 40-41 0.31846391725490447 42-43 0.34159022755991897 44-45 0.3549256562245468 46-47 0.36164294464111585 48-49 0.3703184093857622 50-51 0.3641464358959995 52-53 0.3649644082862091 54-55 0.35056313681009604 56-57 0.3283539470638014 58-59 0.31412618488258126 60-61 0.2701291851061604 62-63 0.34211075544459774 64-65 0.37036798347001737 66-67 0.3748544380951059 68-69 0.38729753324314153 70-71 0.3876941259171825 72-73 0.3710372336074615 74-75 0.3751270955585091 76-77 0.3583710550802778 78-79 0.337128559976958 80-81 0.33561655040717675 82-83 0.3498938866726519 84-85 0.35648723987858316 86-87 0.3497699514620141 88-89 0.36434473223302 90-91 0.388685607602285 92-93 0.38392649551379326 94-95 0.3837034121346452 96-97 0.38866082056015744 98-99 0.3774818645606274 100-101 0.3556940545305012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2017183.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.12993112158955 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15053438269055 24.984040442677195 2 14.094459770683626 10.184637214216284 3 5.979321960964415 6.480974718103562 4 3.109742511183907 4.494191309398139 5 1.8036776459296058 3.2583374556493716 6 1.2078542281240385 2.6183814040225335 7 0.816100756430467 2.0639964882676933 8 0.5808231109520043 1.6788079194018626 9 0.4343676772447817 1.412430683426892 >10 2.4109852935536855 16.469302059911154 >50 0.24962315944267655 6.3194564660649295 >100 0.1509512887317703 9.92048720755856 >500 0.005641509247672989 1.3622129166202426 >1k 0.0044031291689155035 3.454276854331021 >5k 9.631845057002665E-4 2.3806536235244535 >10k+ 5.503911461144379E-4 2.9178132368262206 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23548 1.1673705360396156 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12890 0.6390099460485241 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12171 0.6033661794690913 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10083 0.49985549154439635 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9138 0.45300798192330594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8863 0.4393751087531473 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6896 0.3418628850233221 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6606 0.3274864005893367 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5970 0.29595728300307905 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5337 0.26457688766958676 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5077 0.251687625763255 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4648 0.2304203436178076 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4535 0.2248184720969788 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3920 0.19433041028007872 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3295 0.1633466076206274 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3205 0.1588849400376664 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2839 0.14074082520029169 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2816 0.1396006212624239 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2782 0.13791510239774973 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2711 0.13439534241563605 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2652 0.13147047144458385 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2627 0.1302311193382058 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2565 0.12715752611438824 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2220 0.1100544670463711 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2206 0.10936042986679939 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2071 0.10266792849235791 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.436112638268317E-5 0.0 16-17 0.0 0.0 0.0 1.4872225276536637E-4 0.0 18-19 0.0 0.0 0.0 1.4872225276536637E-4 0.0 20-21 0.0 0.0 0.0 2.726574634031716E-4 0.0 22-23 0.0 0.0 0.0 5.701019689339043E-4 0.0 24-25 0.0 0.0 0.0 0.0011154168957402478 0.0 26-27 0.0 0.0 0.0 0.0022308337914804955 0.0 28-29 0.0 0.0 0.0 0.0058249548999768485 0.0 30-31 0.0 0.0 0.0 0.016185938509297372 0.0 32-33 0.0 0.0 0.0 0.03631301671687695 0.0 34-35 0.0 0.0 0.0 0.06427280023676582 0.0 36-37 0.0 0.0 0.0 0.10931085578254426 0.0 38-39 0.0 0.0 0.0 0.1822343337218289 0.0 40-41 0.0 0.0 0.0 0.263263174436826 0.0 42-43 0.0 0.0 0.0 0.34382106135139945 0.0 44-45 0.0 0.0 0.0 0.4473069622339669 0.0 46-47 0.0 0.0 0.0 0.5730268399049565 0.0 48-49 0.0 0.0 0.0 0.7052161355712396 0.0 50-51 0.0 0.0 0.0 0.8588710097199908 0.0 52-53 0.0 0.0 0.0 1.007147095727061 0.0 54-55 0.0 0.0 0.0 1.1711629534851324 0.0 56-57 0.0 0.0 0.0 1.3813074966425951 0.0 58-59 0.0 0.0 0.0 1.6066712836663803 0.0 60-61 0.0 0.0 0.0 1.852162148897745 0.0 62-63 0.0 0.0 0.0 2.1060310343682254 0.0 64-65 0.0 0.0 0.0 2.3608666144816803 0.0 66-67 0.0 0.0 0.0 2.6600462129613422 0.0 68-69 0.0 0.0 0.0 3.0054784320510333 0.0 70-71 0.0 0.0 0.0 3.3886365292588723 0.0 72-73 0.0 0.0 0.0 3.7685723109901286 0.0 74-75 0.0 0.0 0.0 4.170271115709383 0.0 76-77 0.0 0.0 0.0 4.581760802069024 0.0 78-79 0.0 0.0 0.0 5.097653509869952 0.0 80-81 0.0 2.4787042127561058E-5 0.0 5.65786544899496 0.0 82-83 0.0 4.9574084255122115E-5 0.0 6.195570753868142 0.0 84-85 0.0 4.9574084255122115E-5 0.0 6.753527072159541 2.4787042127561058E-5 86-87 0.0 4.9574084255122115E-5 0.0 7.322538411239833 4.9574084255122115E-5 88-89 0.0 4.9574084255122115E-5 0.0 7.95460798549264 4.9574084255122115E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 75 3.109708E-4 31.613594 4 TATAACG 80 4.5391958E-4 29.637747 2 GTACATG 12890 0.0 27.89391 1 ACCGTCC 105 7.0196445E-5 27.07922 8 TACATGG 13025 0.0 27.050558 2 ACATGGG 13335 0.0 25.710493 3 CATGGGG 4515 0.0 24.156569 4 GTATAGG 530 0.0 22.404259 1 GAGTACT 8695 0.0 21.882702 12-13 CATGGGA 8520 0.0 21.762177 4 AGTACTT 8950 0.0 21.418076 12-13 GTAAGGT 450 0.0 21.075731 4 GTACTTT 9310 0.0 20.335875 14-15 TCTACGG 165 6.4078835E-5 20.117744 2 ATGGGAG 3230 0.0 20.113293 5 GTAAGCG 225 0.0 20.076763 94-95 AGTACAT 9750 0.0 20.038157 2 ATGGGGA 2485 0.0 19.464306 5 TAAGGTG 585 0.0 18.643915 5 ATGGGAT 3135 0.0 18.453869 5 >>END_MODULE