##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2017183 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.222716035183716 33.0 31.0 34.0 31.0 34.0 2 32.40379330977903 34.0 31.0 34.0 31.0 34.0 3 32.49522775077918 34.0 31.0 34.0 30.0 34.0 4 36.03696442018399 37.0 35.0 37.0 35.0 37.0 5 35.981119710011434 37.0 35.0 37.0 35.0 37.0 6 36.042068567898895 37.0 35.0 37.0 35.0 37.0 7 35.98705075345172 37.0 35.0 37.0 35.0 37.0 8 35.97252852121002 37.0 35.0 37.0 35.0 37.0 9 37.64310724411221 39.0 37.0 39.0 35.0 39.0 10-11 37.562024615515796 39.0 37.0 39.0 35.0 39.0 12-13 37.49297931818779 39.0 37.0 39.0 35.0 39.0 14-15 38.70028971094838 40.0 38.0 41.0 34.0 41.0 16-17 38.6807671886983 40.0 38.0 41.0 34.0 41.0 18-19 38.840217273296474 40.0 38.0 41.0 35.0 41.0 20-21 38.844774123121205 40.0 38.0 41.0 34.5 41.0 22-23 38.788008822204034 40.0 38.0 41.0 34.0 41.0 24-25 38.69761642845493 40.0 38.0 41.0 34.0 41.0 26-27 38.49914583852828 40.0 38.0 41.0 34.0 41.0 28-29 38.359175394597315 40.0 38.0 41.0 34.0 41.0 30-31 38.16582878201928 40.0 37.5 41.0 33.5 41.0 32-33 37.93608958631914 40.0 37.0 41.0 33.0 41.0 34-35 37.839967172041405 40.0 37.0 41.0 33.0 41.0 36-37 37.7217371948901 40.0 37.0 41.0 33.0 41.0 38-39 37.600638613353375 40.0 37.0 41.0 33.0 41.0 40-41 37.45061429726505 40.0 36.5 41.0 32.5 41.0 42-43 37.247576446955975 40.0 36.0 41.0 32.0 41.0 44-45 36.97874040183761 40.0 36.0 41.0 31.0 41.0 46-47 36.625703270352766 40.0 36.0 41.0 30.5 41.0 48-49 36.254866068175275 39.0 35.0 41.0 30.0 41.0 50-51 35.99901446720501 39.0 35.0 41.0 29.0 41.0 52-53 35.801337062626445 39.0 35.0 41.0 28.5 41.0 54-55 35.53770753570697 39.0 35.0 40.0 28.0 41.0 56-57 35.28408181111976 38.0 35.0 40.0 27.5 41.0 58-59 34.99995414397206 38.0 34.0 40.0 27.0 41.0 60-61 34.61641531779715 38.0 34.0 40.0 26.0 41.0 62-63 34.28233829057652 37.0 34.0 40.0 26.0 41.0 64-65 33.860912718380035 36.5 33.5 39.5 25.5 41.0 66-67 33.45880244876147 36.0 33.0 39.0 24.5 40.0 68-69 33.06444432656829 36.0 33.0 39.0 23.0 40.0 70-71 32.642678180412986 35.0 32.0 38.0 22.5 40.0 72-73 32.136653441953456 35.0 32.0 37.0 21.5 39.5 74-75 31.650818988658937 35.0 31.0 37.0 20.0 39.0 76-77 29.659901456635318 33.0 28.5 35.0 16.5 37.0 78-79 30.64853238402267 34.0 30.5 36.0 17.5 37.5 80-81 30.677016413483557 34.0 31.0 35.5 17.5 37.0 82-83 30.36091123115751 34.0 31.0 35.0 13.0 37.0 84-85 29.989025784968444 34.0 30.5 35.0 7.5 36.0 86-87 29.510676522655604 34.0 30.0 35.0 3.5 36.0 88-89 29.002784080571764 34.0 29.0 35.0 2.0 35.5 90-91 28.576090766182347 34.0 29.0 35.0 2.0 35.0 92-93 28.248306425346634 34.0 29.0 35.0 2.0 35.0 94-95 27.853114219185866 33.5 28.5 35.0 2.0 35.0 96-97 27.287765909191187 33.0 27.0 35.0 2.0 35.0 98-99 26.58268758957417 33.0 25.5 35.0 2.0 35.0 100-101 23.292731001599755 29.5 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16408935908077638 1101 2 0.19597171813834535 1101 3 0.22703352644790442 1101 4 0.18089472853825583 1101 5 0.186193647131617 1101 6 0.19995090348815125 1101 7 0.2385255986803756 1101 8 0.26102696777928713 1101 9 0.310077909406246 1101 10-11 0.2736020191378685 1101 12-13 0.26952396940699686 1101 14-15 0.4285695856094236 1101 16-17 0.3696189164587196 1101 18-19 0.296171422711744 1101 20-21 0.37294173597077673 1101 22-23 0.28259422557877656 1101 24-25 0.3150860185642159 1101 26-27 0.3780928259193317 1101 28-29 0.3209266063851075 1101 30-31 0.3386690275942499 1101 32-33 0.31137738608116905 1101 34-35 0.3345074365065557 1101 36-37 0.4409977508414684 1101 38-39 0.5651406967363641 1101 40-41 0.41719776385085794 1101 42-43 0.6134892931481701 1101 44-45 0.6730240758419939 1101 46-47 0.4959093485208612 1101 48-49 0.5730634467175904 1101 50-51 0.6018333591299481 1101 52-53 0.46524927606429145 1101 54-55 0.4551814966720471 1101 56-57 0.33317583545803586 1101 58-59 0.324133062946089 1101 60-61 0.29937773137385904 1101 62-63 0.4197530883363072 1101 64-65 0.2913507890500924 1101 66-67 0.4078009025947509 1101 68-69 0.3912260450519156 1101 70-71 0.37062717291181 1101 72-73 0.15659504228013787 1101 74-75 0.2262520478888952 1101 76-77 0.38295805940506966 1101 78-79 0.5700945205580901 1101 80-81 0.5956110307740445 1101 82-83 0.47911354342747003 1101 84-85 0.450214976123096 1101 86-87 0.4971707106281471 1101 88-89 0.5766332214944221 1101 90-91 0.6307608154588671 1101 92-93 0.7199222925330844 1101 94-95 0.7479053538406149 1101 96-97 0.4942577836689459 1101 98-99 0.5483577347656983 1101 100-101 0.3535897249208766 1102 1 0.2006872276899756 1102 2 0.24264679988019822 1102 3 0.24021179114377134 1102 4 0.1994586413555055 1102 5 0.22121449210794708 1102 6 0.220198152529413 1102 7 0.2655797442290577 1102 8 0.2478548056188643 1102 9 0.3032893205051579 1102 10-11 0.32467462003847203 1102 12-13 0.3588774539547259 1102 14-15 0.41459510063129557 1102 16-17 0.3950695405232665 1102 18-19 0.19466232993393362 1102 20-21 0.3362627678774004 1102 22-23 0.30265069009092826 1102 24-25 0.33545676365167765 1102 26-27 0.4246065116580269 1102 28-29 0.4856809311556205 1102 30-31 0.31513813270560576 1102 32-33 0.20470233645792035 1102 34-35 0.40322118262823636 1102 36-37 0.4839388838574763 1102 38-39 0.39022725638383093 1102 40-41 0.40902389940422523 1102 42-43 0.4157995845924134 1102 44-45 0.45505917505637683 1102 46-47 0.4465112512271716 1102 48-49 0.4504569253555104 1102 50-51 0.42836589849130036 1102 52-53 0.4460786427495904 1102 54-55 0.39367163304765285 1102 56-57 0.3132417428408161 1102 58-59 0.10550057690162618 1102 60-61 0.05122712079912617 1102 62-63 0.06321807584216543 1102 64-65 -0.044760000874944694 1102 66-67 -0.07488829206328518 1102 68-69 0.1209492468916622 1102 70-71 0.1079674631355445 1102 72-73 0.3447627078355069 1102 74-75 0.17811589068758238 1102 76-77 0.3457796469636065 1102 78-79 0.4886187668949624 1102 80-81 0.6155200708773378 1102 82-83 0.4700933536707339 1102 84-85 0.4575730279368351 1102 86-87 0.5212026557952569 1102 88-89 0.6643631104282797 1102 90-91 0.7906578432029185 1102 92-93 0.8417030444939257 1102 94-95 0.8256945337240893 1102 96-97 0.5855409541609369 1102 98-99 0.7164973607766285 1102 100-101 0.7129346338003906 1103 1 0.159106581872706 1103 2 0.09543687876545448 1103 3 0.12333554071946651 1103 4 0.08258958649247461 1103 5 0.06755985089270666 1103 6 0.0401555110075833 1103 7 0.0813067383771866 1103 8 0.08339211851471617 1103 9 0.12391148627524728 1103 10-11 0.20967469042982856 1103 12-13 0.17776157882587285 1103 14-15 0.23350972842525408 1103 16-17 0.2079714947438589 1103 18-19 0.11854205143443153 1103 20-21 0.10971490097025338 1103 22-23 0.1217902573874099 1103 24-25 0.2498486683323904 1103 26-27 0.20500767984175639 1103 28-29 0.1976218086699646 1103 30-31 0.16555727415001087 1103 32-33 0.08734163704936293 1103 34-35 0.3039435544103739 1103 36-37 0.24562596141154813 1103 38-39 0.28273073293490114 1103 40-41 0.1849708587321004 1103 42-43 0.17707307379829018 1103 44-45 0.2844109644829729 1103 46-47 0.22129909948842652 1103 48-49 0.2321888030997954 1103 50-51 0.23518885483457552 1103 52-53 0.12328357389902322 1103 54-55 0.030250112375277638 1103 56-57 0.08793417170252837 1103 58-59 -0.039565379802994016 1103 60-61 -0.140066719331152 1103 62-63 -0.16161740390623436 1103 64-65 0.12924665971198834 1103 66-67 0.2289040815803176 1103 68-69 0.09729352634223432 1103 70-71 0.17408591145969154 1103 72-73 0.14000127979815602 1103 74-75 0.2543387841479081 1103 76-77 0.1794046612374096 1103 78-79 0.21569300475205821 1103 80-81 0.2439017109334607 1103 82-83 0.2575399148963591 1103 84-85 0.2577175648633627 1103 86-87 0.23713206421062338 1103 88-89 0.37571270051592265 1103 90-91 0.49589496041079073 1103 92-93 0.4484271400822628 1103 94-95 0.4983202722467759 1103 96-97 0.47073233262712577 1103 98-99 0.4048031658739042 1103 100-101 0.33755268245802483 1104 1 0.16539134170381686 1104 2 0.18983533469126002 1104 3 0.2072987601356715 1104 4 0.1329902391843092 1104 5 0.14687512239272138 1104 6 0.13179754577257086 1104 7 0.21594685052347273 1104 8 0.23251809084825936 1104 9 0.21235493644157089 1104 10-11 0.28788500177687126 1104 12-13 0.26104466178428964 1104 14-15 0.3781136154574227 1104 16-17 0.3441122420329492 1104 18-19 0.19001303262768232 1104 20-21 0.2872430728202531 1104 22-23 0.3086753131302018 1104 24-25 0.3022069181783493 1104 26-27 0.29120727761952026 1104 28-29 0.3083033293175603 1104 30-31 0.22515606240094854 1104 32-33 0.25622774094969714 1104 34-35 0.3248811441921262 1104 36-37 0.35716520139866503 1104 38-39 0.42521408902415914 1104 40-41 0.31409528842646495 1104 42-43 0.4274828746991801 1104 44-45 0.6027652016733072 1104 46-47 0.6563997312828818 1104 48-49 0.5106691061984989 1104 50-51 0.43717951708419633 1104 52-53 0.4428335059302171 1104 54-55 0.39216716442768984 1104 56-57 0.5117113536176561 1104 58-59 0.5222564830414314 1104 60-61 0.40829830736458206 1104 62-63 0.4110380610873605 1104 64-65 0.40908215338792075 1104 66-67 0.35173508157224376 1104 68-69 0.44146992892562054 1104 70-71 0.4121354654545968 1104 72-73 0.4276411524766104 1104 74-75 0.5157992329423031 1104 76-77 0.5540725528472734 1104 78-79 0.6089193538662521 1104 80-81 0.6928361687989373 1104 82-83 0.7842501441785821 1104 84-85 0.7874742075543466 1104 86-87 0.8060823550549117 1104 88-89 1.2008833998187747 1104 90-91 1.0699135384502476 1104 92-93 1.0876923296220937 1104 94-95 0.9719042206676214 1104 96-97 0.9184743842236216 1104 98-99 0.9810131286323234 1104 100-101 0.928431320104341 1105 1 0.07984027483168887 1105 2 0.13090979593356167 1105 3 0.21156387704968438 1105 4 0.155068372354755 1105 5 0.1539679363344746 1105 6 0.14414460661043194 1105 7 0.16163522714714418 1105 8 0.17989579736240557 1105 9 0.1839877297446435 1105 10-11 0.27384730956173087 1105 12-13 0.2388408628310259 1105 14-15 0.2929839410952084 1105 16-17 0.22800457841581334 1105 18-19 0.23919318269778955 1105 20-21 0.2560908289412964 1105 22-23 0.2890916963619645 1105 24-25 0.31913058510878756 1105 26-27 0.24771824018760213 1105 28-29 0.32144444172437403 1105 30-31 0.3539151901618425 1105 32-33 0.34455155318390496 1105 34-35 0.44012404212315914 1105 36-37 0.4919800027165806 1105 38-39 0.4350659104115735 1105 40-41 0.6142586394831611 1105 42-43 0.5271579657454097 1105 44-45 0.5784891464856372 1105 46-47 0.6565582234554483 1105 48-49 0.7142199093026349 1105 50-51 0.8465623404660718 1105 52-53 0.9135366038159063 1105 54-55 0.8218961312438253 1105 56-57 0.8207096310275404 1105 58-59 0.7775387842089572 1105 60-61 0.6265842550089502 1105 62-63 0.6504133011393733 1105 64-65 0.9449625542689972 1105 66-67 0.9814043824482326 1105 68-69 1.0153375486277056 1105 70-71 0.880940272510621 1105 72-73 0.7316593628801726 1105 74-75 1.0439794593663088 1105 76-77 0.9067566814179742 1105 78-79 0.8305602453095275 1105 80-81 0.9618296335997911 1105 82-83 1.0902628108267365 1105 84-85 1.1289499316437706 1105 86-87 1.163821024599887 1105 88-89 1.2986265059877056 1105 90-91 1.3944207666188184 1105 92-93 1.427372580340517 1105 94-95 1.526844172958004 1105 96-97 1.5454853259679169 1105 98-99 1.7587079004730057 1105 100-101 1.6342128716154534 1106 1 0.07878635130118283 1106 2 0.09466060749915073 1106 3 0.09437102683133958 1106 4 0.0903215585834829 1106 5 0.12285495751078201 1106 6 0.08775024799182773 1106 7 0.12332766380055915 1106 8 0.07615659229645644 1106 9 0.050903202409330106 1106 10-11 0.15529619871743705 1106 12-13 0.14517757805822384 1106 14-15 0.2443528187778199 1106 16-17 0.2369006713389581 1106 18-19 0.18126991233305034 1106 20-21 0.29013016874936426 1106 22-23 0.21945492199053973 1106 24-25 0.21638137888663067 1106 26-27 0.20263577178307202 1106 28-29 0.21636809897243126 1106 30-31 0.1986790912798 1106 32-33 0.2983851444394574 1106 34-35 0.21312470026950336 1106 36-37 0.08667608807994043 1106 38-39 0.2651349157212124 1106 40-41 0.2541147366753691 1106 42-43 0.3069185205449898 1106 44-45 0.261540266223804 1106 46-47 0.2658638790670338 1106 48-49 -0.03244155445040775 1106 50-51 -0.06823466808094025 1106 52-53 8.296545910084774E-4 1106 54-55 0.15353962938056043 1106 56-57 0.1268696917726757 1106 58-59 0.11495440362249099 1106 60-61 0.11349803783844692 1106 62-63 0.10475884575547667 1106 64-65 -0.016513928116694387 1106 66-67 -0.017816014307285855 1106 68-69 -0.021932489260187538 1106 70-71 0.05823733664431785 1106 72-73 0.0784560690356173 1106 74-75 0.10022178840389628 1106 76-77 0.19308091187592424 1106 78-79 0.32626339462870035 1106 80-81 0.256398758976669 1106 82-83 0.19817685380191463 1106 84-85 0.10392980926412676 1106 86-87 0.08368920633721544 1106 88-89 0.38736897955067917 1106 90-91 0.5287754496857815 1106 92-93 0.49905772402097526 1106 94-95 0.4032480146649142 1106 96-97 0.4472430880924847 1106 98-99 0.4365458230429766 1106 100-101 0.26202611369768647 1107 1 -0.0028707994048033925 1107 2 0.05309448506740466 1107 3 0.07064606134373008 1107 4 -0.010655851632179747 1107 5 0.06269997642245073 1107 6 0.06628896413614171 1107 7 0.060997987738723225 1107 8 0.09074318914199608 1107 9 0.01286153955065572 1107 10-11 0.16943906479330906 1107 12-13 0.20107442728916425 1107 14-15 0.075622089199463 1107 16-17 0.14050968159888555 1107 18-19 0.14161085683156927 1107 20-21 0.10297599415273595 1107 22-23 0.10264443364351195 1107 24-25 0.11151581287537482 1107 26-27 0.20227768733179374 1107 28-29 0.1652117266992903 1107 30-31 0.17541201960359132 1107 32-33 0.18322758995316235 1107 34-35 0.11349892524381033 1107 36-37 0.1339978836115776 1107 38-39 0.10896880836109091 1107 40-41 0.11847288098554287 1107 42-43 0.14929442156830675 1107 44-45 0.18377234022221955 1107 46-47 0.146480259987662 1107 48-49 0.216210150699645 1107 50-51 0.02395844953590398 1107 52-53 0.1375560518819654 1107 54-55 0.05719567162811501 1107 56-57 -0.06261884605093826 1107 58-59 -0.08634450764043322 1107 60-61 -0.16641911638425455 1107 62-63 0.12492701717851418 1107 64-65 0.13670025395160934 1107 66-67 0.2222865581820983 1107 68-69 0.3493388227772769 1107 70-71 0.25261332638707046 1107 72-73 0.23939685843546243 1107 74-75 0.18452729429973047 1107 76-77 0.20296765029843655 1107 78-79 0.5093543796121374 1107 80-81 0.30825633542903574 1107 82-83 0.38025571575608197 1107 84-85 0.3860721644015932 1107 86-87 0.44360015554427434 1107 88-89 0.6104367531793109 1107 90-91 0.4663748248016901 1107 92-93 0.5728108285330009 1107 94-95 0.7499680947048084 1107 96-97 0.7420788689597657 1107 98-99 0.9116132578275185 1107 100-101 0.8122999681347558 1108 1 -0.04276626452460164 1108 2 -0.024702427226017676 1108 3 0.0405091992028801 1108 4 -0.012921781116453701 1108 5 -0.007997023486915111 1108 6 -0.023275675278945585 1108 7 -0.09166523471512988 1108 8 -0.024230072890091492 1108 9 -0.017743614683610076 1108 10-11 -0.006736952609031732 1108 12-13 -0.01335047980771975 1108 14-15 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-0.8161014421220045 2316 60-61 -0.8667206467249926 2316 62-63 -0.8128622575012301 2316 64-65 -1.011256595166934 2316 66-67 -0.9922782020577685 2316 68-69 -0.7955999842386703 2316 70-71 -0.8948024197620086 2316 72-73 -0.748599124530692 2316 74-75 -0.7289382590241367 2316 76-77 -0.809335305849153 2316 78-79 -0.7895737793568998 2316 80-81 -0.933308904714508 2316 82-83 -0.9329077594880104 2316 84-85 -1.0170893642622225 2316 86-87 -0.9346823286460832 2316 88-89 -1.2433613746486536 2316 90-91 -1.4869163073454068 2316 92-93 -1.4432851151223502 2316 94-95 -1.2212143011310275 2316 96-97 -1.2920947803263019 2316 98-99 -1.4311006332137062 2316 100-101 -0.5189299419559923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 27.0 5 47.0 6 135.0 7 302.0 8 641.0 9 1224.0 10 1991.0 11 3206.0 12 4577.0 13 6882.0 14 8178.0 15 11268.0 16 14679.0 17 20743.0 18 19464.0 19 12107.0 20 11021.0 21 11454.0 22 12328.0 23 13464.0 24 15486.0 25 18213.0 26 21769.0 27 26552.0 28 32070.0 29 39427.0 30 47860.0 31 58072.0 32 71977.0 33 91643.0 34 125952.0 35 182433.0 36 278693.0 37 448037.0 38 375387.0 39 29864.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.291174673685184 19.573569904221998 14.646672277370948 21.488583144721872 2 15.290828843986887 22.45839866784521 38.533588672916636 23.717183815251268 3 20.880901732762965 25.870335016703986 30.020330331953026 23.22843291858002 4 12.5800187687483 16.71097763564337 37.90295674710723 32.806046848501104 5 12.738457542027671 39.31874301934926 33.58426082313801 14.358538615485061 6 30.271175198283945 36.904931282883105 17.18723586308233 15.636657655750618 7 27.764957368766247 34.27686035426632 20.59629691505431 17.361885361913124 8 26.207141345133287 33.19044429781532 19.8185291071757 20.783885249875695 9 26.45193817318508 17.98785732380255 21.378625538684393 34.181578964327976 10-11 25.175157633194413 26.604229760016818 26.708781503710867 21.511831103077906 12-13 27.29241719764642 24.376494348802265 27.126418376518146 21.204670077033168 14-15 23.095914207073793 25.77833125778331 27.606195120210042 23.519559414932857 16-17 21.08074156349203 29.204744963303796 27.7859258798401 21.92858759336407 18-19 20.84173820620142 28.364283260368545 30.08904001273062 20.70493852069941 20-21 22.391795885648452 26.290921547524444 29.427126839756234 21.89015572707087 22-23 21.562669326481533 26.417632906880534 29.90727663280922 22.11242113382871 24-25 22.47919249685576 26.648823283582384 29.87622330370948 20.995760915852372 26-27 21.94276159123758 26.002975966947584 31.156658657880055 20.897603783934784 28-29 20.721868070472535 27.911275278445235 30.55161579291517 20.81524085816706 30-31 21.294027363903027 26.552697499433613 30.085346743453616 22.067928393209737 32-33 21.31616219252294 27.045984424814208 30.095435069599535 21.542418313063315 34-35 22.491230688117387 26.373325923426943 29.88189346243492 21.253549926020742 36-37 22.30757101624043 25.80630685799033 30.730400340745067 21.155721785024177 38-39 21.47469298919699 26.881999771941917 30.56930239014789 21.07400484871321 40-41 21.29880207455301 27.193056395712063 30.221189793833858 21.28695173590107 42-43 21.086032073790292 27.38968824791436 29.05636524310664 22.467914435188707 44-45 21.560263963673886 26.514503104966536 29.143902737075155 22.781330194284422 46-47 22.54061484814699 27.534266880364232 28.708405443041553 21.216712828447225 48-49 22.291334003905448 27.24933731842872 28.743574579004484 21.715754098661353 50-51 20.91746261990112 27.570626958486166 29.756497055547264 21.755413366065447 52-53 21.957997861374004 28.40476050016285 27.900790359625276 21.736451278837865 54-55 20.963863962763917 27.240934511147476 28.618449590344554 23.176751935744054 56-57 22.066248343915337 27.28431859784625 28.19271434290155 22.456718715336862 58-59 23.049102358051414 26.77357035139259 28.09375889239104 22.083568398164957 60-61 21.872794681381887 27.435347215526136 29.439595308308963 21.25226279478301 62-63 21.38801396322693 27.41164476412453 29.281679077294736 21.918662195353804 64-65 21.009819138868412 28.385153949839946 28.569123376510706 22.03590353478093 66-67 21.514780761091085 27.452367980495573 27.885422393506193 23.14742886490715 68-69 20.97075437487157 27.56998840208837 28.44638797802965 23.012869245010407 70-71 22.088829344890705 27.553605090268235 28.046247674665032 22.311317890176024 72-73 21.89372506113724 26.7531503091192 28.86475842796613 22.488366201777428 74-75 20.90239209828756 28.972904292768675 27.778391945599385 22.346311663344377 76-77 21.378617457207636 27.224186822103313 28.074816888083724 23.322378832605324 78-79 21.453606341120267 27.47393270714655 28.31800585271639 22.754455099016795 80-81 22.030120550615447 27.67262999829959 27.95592419246654 22.341325258618426 82-83 21.71466346880774 27.05195810196695 28.643980243735946 22.58939818548937 84-85 21.11970009661989 27.65351482736073 28.211173701146596 23.01561137487278 86-87 21.3089571377812 27.658418308100607 28.020855457871242 23.01176909624695 88-89 21.114714102777135 27.542414198993264 27.49251514135413 23.850356556875475 90-91 21.33936164893801 26.726538559985112 28.03945336218474 23.894646428892134 92-93 21.969102456247153 27.329052445910957 27.873450252158577 22.828394845683313 94-95 21.424258483241232 26.757116235859613 28.0926420656926 23.725983215206554 96-97 20.489886143200692 26.82664388902742 28.89420543401367 23.789264533758217 98-99 21.187343934586004 27.438115431272227 27.400116895690672 23.974423738451097 100-101 20.755208514072628 26.27960943456651 28.46506356900581 24.500118482355052 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2056.0 1 1389.5 2 1002.0 3 1335.5 4 1168.5 5 1104.5 6 2075.5 7 4211.0 8 6124.5 9 6383.0 10 5184.5 11 4080.5 12 3922.5 13 3993.0 14 4057.5 15 4093.5 16 4053.5 17 4138.0 18 4286.0 19 4435.5 20 4666.5 21 5055.5 22 5710.0 23 6510.0 24 7316.0 25 8507.0 26 10331.5 27 12610.0 28 14259.5 29 16396.5 30 20330.0 31 24432.0 32 28935.5 33 33848.0 34 38443.0 35 42233.5 36 47211.0 37 51905.5 38 54581.5 39 55534.5 40 55474.0 41 58797.0 42 62049.5 43 64877.0 44 67784.5 45 70319.5 46 71196.0 47 86176.0 48 126200.5 49 121664.0 50 81408.0 51 66172.5 52 61134.5 53 59570.0 54 57965.5 55 53081.5 56 46151.5 57 40888.5 58 37668.5 59 33080.0 60 28132.0 61 24047.0 62 20370.0 63 15813.5 64 11684.0 65 9181.0 66 6952.5 67 5755.5 68 4468.0 69 2805.5 70 2069.0 71 1681.5 72 1385.0 73 1218.0 74 1008.0 75 843.5 76 555.0 77 272.0 78 151.5 79 94.0 80 63.5 81 40.0 82 24.5 83 11.5 84 5.0 85 3.0 86 2.5 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.275235315784438 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023299819599907393 16-17 6.196760531890264E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.914816851024424E-5 26-27 0.0011649909799953697 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0031479543502002548 36-37 0.02111855989268202 38-39 0.007584834891033685 40-41 0.01799539258460933 42-43 0.005725806731466605 44-45 5.701019689339043E-4 46-47 9.914816851024424E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.478704212756106E-5 58-59 4.4616675829609905E-4 60-61 0.00210689858084269 62-63 0.0013632873170158584 64-65 0.0 66-67 0.0 68-69 4.7095380042366016E-4 70-71 2.478704212756106E-5 72-73 0.0 74-75 0.0 76-77 0.2288339729216437 78-79 0.0 80-81 0.0010906298536126867 82-83 0.0 84-85 0.0 86-87 0.1028662248293784 88-89 0.3532153503177451 90-91 0.35264524834881117 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.4787042127561056E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2017183.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.58398561134057 #Duplication Level Percentage of deduplicated Percentage of total 1 67.53840437261123 23.357472050352875 2 14.692011164919707 10.162166054584764 3 6.270412700356716 6.505675878189115 4 3.3058382344374935 4.573162477328232 5 1.9529305782252138 3.377006150864391 6 1.237070970774684 2.566970679208727 7 0.8589724612092049 2.079468386729786 8 0.6425500938414985 1.7777554559983935 9 0.47250882855280557 1.4707114675111457 >10 2.586216759934964 16.901461401779365 >50 0.2663188400459663 6.435634311445587 >100 0.16429294835269953 10.326094433955891 >500 0.006307701798476536 1.4797203738784908 >1k 0.004444062630744833 3.2742147427229575 >5k 0.001290211731506564 3.221016995581913 >10k+ 4.300705771688547E-4 2.491469139868518 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 25442 1.2612638516188168 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13132 0.6510068744382637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11679 0.5789757300155712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9742 0.4829507288133997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9561 0.4739778195632226 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8682 0.43040219950297026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7764 0.3848931901567681 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6785 0.3363601616710036 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5840 0.28951265204991317 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5727 0.28391078052908436 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5709 0.2830184470124922 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5158 0.2557031265879199 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4491 0.22263721238975345 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4450 0.22060467493529345 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3556 0.17628544361121426 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3163 0.15680282849895127 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2914 0.14445888151942585 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2878 0.14267421448624146 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2810 0.13930317675689313 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2697 0.13370130523606436 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2558 0.12681050752460238 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2557 0.12676093344034725 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2437 0.1208120433297326 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.11912652446505845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.11302891210167842 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2213 0.10970744845658524 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2191 0.10861681860297256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.436112638268317E-5 0.0 16-17 0.0 0.0 0.0 1.4872225276536637E-4 0.0 18-19 0.0 0.0 0.0 1.4872225276536637E-4 0.0 20-21 0.0 0.0 0.0 2.726574634031716E-4 0.0 22-23 0.0 0.0 0.0 5.701019689339043E-4 0.0 24-25 0.0 0.0 0.0 0.0011649909799953699 0.0 26-27 0.0 0.0 0.0 0.0022804078757356176 0.0 28-29 0.0 0.0 0.0 0.00584974194210441 0.0 30-31 0.0 0.0 0.0 0.016359447804190297 0.0 32-33 0.0 0.0 0.0 0.036560887138152566 0.0 34-35 0.0 0.0 0.0 0.06509077262697535 4.9574084255122115E-5 36-37 0.0 0.0 0.0 0.11059978197317745 4.9574084255122115E-5 38-39 0.0 0.0 0.0 0.18374634329161013 4.9574084255122115E-5 40-41 0.0 0.0 0.0 0.265692304565327 4.9574084255122115E-5 42-43 0.0 0.0 0.0 0.34691944161734456 4.9574084255122115E-5 44-45 0.0 0.0 0.0 0.4513224630586318 4.9574084255122115E-5 46-47 0.0 0.0 0.0 0.5776372297406829 4.9574084255122115E-5 48-49 0.0 0.0 0.0 0.7101239699124968 4.9574084255122115E-5 50-51 0.0 0.0 0.0 0.8648198998306054 4.9574084255122115E-5 52-53 0.0 0.0 0.0 1.0136660878066095 4.9574084255122115E-5 54-55 0.0 0.0 0.0 1.1783264086599976 4.9574084255122115E-5 56-57 0.0 0.0 0.0 1.389784665050221 4.9574084255122115E-5 58-59 0.0 0.0 0.0 1.616288656011874 4.9574084255122115E-5 60-61 0.0 0.0 0.0 1.8636881234870608 4.9574084255122115E-5 62-63 0.0 0.0 0.0 2.118598064726899 4.9574084255122115E-5 64-65 0.0 0.0 0.0 2.375119163705028 4.9574084255122115E-5 66-67 0.0 0.0 0.0 2.676083429217875 4.9574084255122115E-5 68-69 0.0 0.0 0.0 3.022556704076923 4.9574084255122115E-5 70-71 0.0 0.0 0.0 3.4068054311383746 4.9574084255122115E-5 72-73 0.0 0.0 0.0 3.7875591852598403 4.9574084255122115E-5 74-75 0.0 0.0 0.0 4.191662333065468 4.9574084255122115E-5 76-77 0.0 0.0 0.0 4.605630723637866 4.9574084255122115E-5 78-79 0.0 0.0 0.0 5.123754265230274 4.9574084255122115E-5 80-81 0.0 0.0 0.0 5.687188519831865 4.9574084255122115E-5 82-83 0.0 0.0 0.0 6.227719547507588 4.9574084255122115E-5 84-85 0.0 0.0 0.0 6.787559681000682 4.9574084255122115E-5 86-87 0.0 0.0 0.0 7.361330132169465 4.9574084255122115E-5 88-89 0.0 0.0 0.0 7.996820318235876 4.9574084255122115E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12450 0.0 26.177923 1 GTATAGG 500 0.0 25.69258 1 TACATGG 12595 0.0 25.505243 2 ACATGGG 12585 0.0 24.281284 3 AGTACTT 10180 0.0 22.678083 12-13 GAGTACT 9975 0.0 21.62182 12-13 CATGGGA 8100 0.0 21.61625 4 CATGGGG 4260 0.0 21.386053 4 GTACTTT 10450 0.0 20.457369 14-15 GATAGTC 260 1.5097612E-8 20.075129 7 AGTACAT 9560 0.0 19.406975 2 TACTTTT 11905 0.0 19.272512 14-15 TAGGACA 740 0.0 19.236609 4 ATGGGAG 2910 0.0 19.077957 5 GATATAG 1025 0.0 19.03154 1 ATATAGG 985 0.0 18.787428 2 CATATAG 460 0.0 18.567509 3 ATGGGAT 3205 0.0 18.358307 5 AACCGAT 130 0.0076108607 18.250116 7 ATGGGGA 2370 0.0 18.219313 5 >>END_MODULE