FastQCFastQC Report
Fri 27 May 2016
SRR939309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.2294900072725969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.17991090695406614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35920.13410250628325496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.11860903743371408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131350.025.9068011
GTACATG106100.025.1835961
GGTATCA94900.024.7050321
TACATGG108400.024.191052
ACATGGG110700.022.7460383
ATCAACG150850.022.350293
TCAACGC152200.022.1512174
CAACGCA153400.021.9466115
TATCAAC154800.021.779982
AACGCAG156900.021.5340086
CATGGGG50500.020.9390074
TACCGTA1150.003776346620.6029997
GAGTACT70000.020.17120612-13
ACGCAGA175450.019.1492237
CGCAGAG175750.018.9817248
AGAGTAC134200.018.67689110-11
AGTACTT74950.018.52292612-13
GTACCGT2051.623677E-518.4924496
GTACTTT75350.018.49162914-15
GTGTACG3609.822543E-1118.4565371
Produced by FastQC (version 0.11.5)