##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2678548 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851811130508022 33.0 31.0 34.0 30.0 34.0 2 31.917865948267494 33.0 31.0 34.0 30.0 34.0 3 31.974439883100843 34.0 31.0 34.0 30.0 34.0 4 35.44874723170912 37.0 35.0 37.0 33.0 37.0 5 35.48021204025464 37.0 35.0 37.0 33.0 37.0 6 35.56277020236337 37.0 35.0 37.0 33.0 37.0 7 35.4939803206812 37.0 35.0 37.0 33.0 37.0 8 35.478822108097376 37.0 35.0 37.0 33.0 37.0 9 37.09917014740822 39.0 37.0 39.0 33.0 39.0 10-11 36.993411729041256 39.0 37.0 39.0 33.0 39.0 12-13 36.89372451044372 39.0 37.0 39.0 33.0 39.0 14-15 38.135113315124464 40.0 38.0 41.0 33.0 41.0 16-17 38.05444946291797 40.0 38.0 41.0 33.0 41.0 18-19 37.96850495118997 40.0 38.0 41.0 33.0 41.0 20-21 37.934602814659286 40.0 38.0 41.0 33.0 41.0 22-23 37.93872445070986 40.0 38.0 41.0 33.0 41.0 24-25 37.86874063858478 40.0 38.0 41.0 32.0 41.0 26-27 37.78376250864274 40.0 38.0 41.0 32.0 41.0 28-29 37.641882841001916 40.0 38.0 41.0 32.0 41.0 30-31 37.47441020284124 40.0 37.0 41.0 32.0 41.0 32-33 37.26824085288 40.0 37.0 41.0 31.0 41.0 34-35 36.987632105155484 40.0 36.5 41.0 30.5 41.0 36-37 36.76242464200753 40.0 36.0 41.0 30.0 41.0 38-39 36.18544506202615 39.5 35.5 40.5 29.0 41.0 40-41 36.173635118728505 39.0 35.0 40.0 30.0 41.0 42-43 36.37992542974776 39.5 36.0 41.0 30.0 41.0 44-45 36.30399641895534 40.0 36.0 41.0 29.5 41.0 46-47 36.05949753373843 39.5 35.5 41.0 28.5 41.0 48-49 35.842601663289216 39.0 35.0 41.0 27.5 41.0 50-51 34.7320077892948 38.0 34.0 40.0 25.5 40.5 52-53 34.83309595347927 38.5 34.5 39.5 26.0 40.5 54-55 35.331720208112756 39.0 35.0 40.5 26.0 41.0 56-57 35.24860950783783 39.0 35.0 41.0 26.0 41.0 58-59 35.04817983474629 39.0 35.0 40.5 26.0 41.0 60-61 34.742565188303516 38.0 34.0 40.0 26.0 41.0 62-63 34.36131553363987 38.0 34.0 40.0 24.5 41.0 64-65 33.97454199066061 37.0 34.0 40.0 23.0 41.0 66-67 33.60753512724058 37.0 33.5 39.5 22.5 41.0 68-69 33.19674988090563 36.0 33.0 39.0 22.0 41.0 70-71 32.77802749848052 36.0 32.5 39.0 20.5 40.0 72-73 32.32800550895485 35.0 32.0 38.0 20.0 40.0 74-75 31.85653047845325 35.0 32.0 37.0 18.0 39.5 76-77 31.382449185155536 35.0 31.0 37.0 16.0 39.0 78-79 30.983629563479916 35.0 31.0 36.0 12.0 39.0 80-81 30.56945199414011 35.0 31.0 36.0 7.5 37.5 82-83 30.139047349534152 34.0 30.0 35.5 6.0 37.0 84-85 29.7166246414102 34.0 30.0 35.0 2.0 37.0 86-87 29.289364610975795 34.0 29.5 35.0 2.0 36.0 88-89 28.860600780721498 34.0 29.0 35.0 2.0 36.0 90-91 28.450995464707 34.0 29.0 35.0 2.0 35.5 92-93 27.999556102784044 34.0 28.0 35.0 2.0 35.0 94-95 27.518093944928374 34.0 27.0 35.0 2.0 35.0 96-97 26.907193001581454 33.5 25.5 35.0 2.0 35.0 98-99 26.118427035841805 33.0 24.5 35.0 2.0 35.0 100-101 23.397612251115156 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27682369293836473 1101 2 0.33691142029138277 1101 3 0.3671299873215581 1101 4 0.29618667691295997 1101 5 0.2881066388640221 1101 6 0.36391364585117714 1101 7 0.22017734853070436 1101 8 0.16879399766050796 1101 9 0.4844203757342882 1101 10-11 0.5345846606336409 1101 12-13 0.42695952483159516 1101 14-15 0.5074418934684815 1101 16-17 0.6132529000392211 1101 18-19 0.6970139422380583 1101 20-21 0.7387666849539656 1101 22-23 0.6716513241639746 1101 24-25 0.6382949315473425 1101 26-27 0.5005989978135617 1101 28-29 0.6076020676624196 1101 30-31 0.6517285886903608 1101 32-33 0.5894755750678016 1101 34-35 0.609289745703947 1101 36-37 0.5013081621695576 1101 38-39 0.71685455967976 1101 40-41 0.6445359079816484 1101 42-43 0.4827138993635174 1101 44-45 0.4524981807568196 1101 46-47 0.5886761289371307 1101 48-49 0.505189898799749 1101 50-51 0.6036878126652994 1101 52-53 0.5325621901864466 1101 54-55 0.5658701919052476 1101 56-57 0.48283518276620896 1101 58-59 0.49866028488025194 1101 60-61 0.5051557620607454 1101 62-63 0.6389640153827756 1101 64-65 0.43655360189772097 1101 66-67 0.3146714795741943 1101 68-69 0.4582585488628297 1101 70-71 0.42926413287105447 1101 72-73 0.4019141231693979 1101 74-75 0.378876702559225 1101 76-77 0.47286885326090555 1101 78-79 0.477932081875192 1101 80-81 0.36757345148171083 1101 82-83 0.46750773242365895 1101 84-85 0.5788661821319181 1101 86-87 0.6208996001138161 1101 88-89 0.5079657890500151 1101 90-91 0.5998930298717546 1101 92-93 0.7072549543388185 1101 94-95 0.6682548824325281 1101 96-97 0.727935742785931 1101 98-99 1.1274800142562924 1101 100-101 1.1966741944482138 1102 1 0.28375026665649017 1102 2 0.3106337851599399 1102 3 0.30941860318568004 1102 4 0.32061298466675225 1102 5 0.25120121290417785 1102 6 0.2757349695772078 1102 7 0.311935042088038 1102 8 0.25746147324210966 1102 9 0.4083693134583797 1102 10-11 0.4111899710068272 1102 12-13 0.3645576441043801 1102 14-15 0.4976093000431163 1102 16-17 0.4933040658255692 1102 18-19 0.4679773225440442 1102 20-21 0.41212906350890677 1102 22-23 0.5337071277931642 1102 24-25 0.4802050154770612 1102 26-27 0.3752899041337372 1102 28-29 0.4160594098593222 1102 30-31 0.395556958314657 1102 32-33 0.3298633007421259 1102 34-35 0.2576602828943919 1102 36-37 0.3624411773999654 1102 38-39 0.725183017000397 1102 40-41 0.5793741082225949 1102 42-43 0.44899717006344986 1102 44-45 0.40924195791034634 1102 46-47 0.40427030043667145 1102 48-49 0.36709133529465987 1102 50-51 0.343826466339479 1102 52-53 0.33837839877148923 1102 54-55 0.4341612417023981 1102 56-57 0.39032937468032003 1102 58-59 0.49132254468570835 1102 60-61 0.44131246249290257 1102 62-63 0.4114782552567178 1102 64-65 0.3808008293992913 1102 66-67 0.40405436326008726 1102 68-69 0.35097788317568046 1102 70-71 0.392913637889869 1102 72-73 0.4266369452090686 1102 74-75 0.31544888240286895 1102 76-77 0.19899603884075034 1102 78-79 0.205635824927775 1102 80-81 0.19199494312467635 1102 82-83 0.20792978187289535 1102 84-85 0.26697879861710305 1102 86-87 0.06860562183375762 1102 88-89 0.05436021127485802 1102 90-91 0.07902705001603749 1102 92-93 0.11465372256456163 1102 94-95 0.11146323169975858 1102 96-97 0.18345302902968186 1102 98-99 0.536964086587087 1102 100-101 0.19291493242015179 1103 1 0.35529544591915396 1103 2 0.43452374055098986 1103 3 0.36299325048745246 1103 4 0.2991932890926279 1103 5 0.23575310316270048 1103 6 0.35674303899109816 1103 7 0.41863814098802976 1103 8 0.38446417485085504 1103 9 0.4828919637333726 1103 10-11 0.41131347160334997 1103 12-13 0.33220721616374504 1103 14-15 0.5194989176002807 1103 16-17 0.5191790786182864 1103 18-19 0.45162858802234496 1103 20-21 0.5412002009148722 1103 22-23 0.5900094119256281 1103 24-25 0.5834302667542985 1103 26-27 0.5863222345536983 1103 28-29 0.5137912306600398 1103 30-31 0.4457554799903818 1103 32-33 0.5439566753379452 1103 34-35 0.41636005444927093 1103 36-37 0.41631505912198463 1103 38-39 0.8798013373002078 1103 40-41 0.7179309767503241 1103 42-43 0.5911414874601562 1103 44-45 0.42574801182990996 1103 46-47 0.397982836375796 1103 48-49 0.430479100962593 1103 50-51 0.2927465310874382 1103 52-53 0.414028755568701 1103 54-55 0.42093959540314785 1103 56-57 0.5518234468287488 1103 58-59 0.444545210860845 1103 60-61 0.780975577654246 1103 62-63 0.48362154923629674 1103 64-65 0.4601279627370829 1103 66-67 0.5265507538555525 1103 68-69 0.461846183556041 1103 70-71 0.36393048248592663 1103 72-73 0.4681074716810514 1103 74-75 0.2221901735303362 1103 76-77 0.21374608248648386 1103 78-79 0.3887125780253804 1103 80-81 0.3282527528774537 1103 82-83 0.26730692100061404 1103 84-85 0.28340278439833355 1103 86-87 0.20783939757367165 1103 88-89 0.2197482262457875 1103 90-91 0.18657154652845165 1103 92-93 0.2495453235678653 1103 94-95 0.26018681098253893 1103 96-97 0.2923385915177974 1103 98-99 0.1923746698534039 1103 100-101 -0.2932005019495918 1104 1 0.27458159632891466 1104 2 0.23346807198305797 1104 3 0.2500330318673072 1104 4 0.20957600730111636 1104 5 0.2775518306740068 1104 6 0.19674665947814418 1104 7 0.24525976634125612 1104 8 0.20554154588634077 1104 9 0.3909827295481989 1104 10-11 0.3637066237691471 1104 12-13 0.38163197804512805 1104 14-15 0.37148900932951534 1104 16-17 0.3786821923394186 1104 18-19 0.4413986666400973 1104 20-21 0.37575774978377297 1104 22-23 0.5012471378097629 1104 24-25 0.40398476377762194 1104 26-27 0.4068976976743315 1104 28-29 0.5392847265675655 1104 30-31 0.44615306878691285 1104 32-33 0.41304973310516147 1104 34-35 0.5055355140925997 1104 36-37 0.35966491987641547 1104 38-39 0.7942018756686764 1104 40-41 0.6619908722880226 1104 42-43 0.6189762827952521 1104 44-45 0.6447769951582814 1104 46-47 0.5108295312495343 1104 48-49 0.5935352522621358 1104 50-51 0.45593001307551617 1104 52-53 0.5129884791843935 1104 54-55 0.5569272366558309 1104 56-57 0.5069866806805052 1104 58-59 0.5317879542900243 1104 60-61 0.505455746283026 1104 62-63 0.4624805639933598 1104 64-65 0.443345113209233 1104 66-67 0.5111135698172049 1104 68-69 0.45884675322025004 1104 70-71 0.48354743700177494 1104 72-73 0.3959452329518598 1104 74-75 0.4612844790360384 1104 76-77 0.44350638374373474 1104 78-79 0.3020788741546312 1104 80-81 0.20468547880760823 1104 82-83 0.11950769643939196 1104 84-85 0.3190213712015577 1104 86-87 0.1475040831998271 1104 88-89 0.16138730459660522 1104 90-91 0.3047812156834375 1104 92-93 0.40625590653787214 1104 94-95 0.42527769645753466 1104 96-97 0.37810552624368654 1104 98-99 0.4316034652028158 1104 100-101 0.052354276326255444 1105 1 0.21965408072392734 1105 2 0.2624089016477136 1105 3 0.40771688765823555 1105 4 0.3150350815908851 1105 5 0.37643827001344476 1105 6 0.31034136845133276 1105 7 0.33045374064325017 1105 8 0.42884073075761364 1105 9 0.4026569788452079 1105 10-11 0.525263930478232 1105 12-13 0.526263027165534 1105 14-15 0.6252639457590305 1105 16-17 0.5994222596423242 1105 18-19 0.6084296997529037 1105 20-21 0.6511161537231942 1105 22-23 0.6311679808936859 1105 24-25 0.63377678767025 1105 26-27 0.6309510307236579 1105 28-29 0.5651685411131098 1105 30-31 0.7360820157162493 1105 32-33 0.6350054579023166 1105 34-35 0.4622364195867945 1105 36-37 0.5609345384599393 1105 38-39 0.9732403807141381 1105 40-41 0.8103031843561013 1105 42-43 0.5838499373584369 1105 44-45 0.43580291423149475 1105 46-47 0.5750506503676647 1105 48-49 0.6271892054767036 1105 50-51 0.5921619960584081 1105 52-53 0.6519188393649884 1105 54-55 0.863556608206018 1105 56-57 0.8366502081156142 1105 58-59 0.9603336448418887 1105 60-61 0.8781718802937135 1105 62-63 0.880791155890357 1105 64-65 0.6722831835000065 1105 66-67 0.7242874662048067 1105 68-69 0.706412856361446 1105 70-71 0.7470975764389038 1105 72-73 0.8035581679929678 1105 74-75 0.8353547233358256 1105 76-77 0.8633859459488953 1105 78-79 0.9540643001422531 1105 80-81 0.9942924987838637 1105 82-83 0.9272208080481583 1105 84-85 1.0521041501589252 1105 86-87 0.8250691471382332 1105 88-89 0.8612582756283409 1105 90-91 0.8931075634439374 1105 92-93 0.7841793502106604 1105 94-95 0.6311259392850168 1105 96-97 0.8411041781846578 1105 98-99 0.8343637750143422 1105 100-101 0.49115305794166275 1106 1 0.09531978030197763 1106 2 0.13096436399380806 1106 3 0.16953167240127343 1106 4 0.09356969505986967 1106 5 0.09121760829700065 1106 6 0.09386458523847097 1106 7 0.04494783652359047 1106 8 0.08788633692987702 1106 9 0.1144186882908329 1106 10-11 0.20350292074155618 1106 12-13 0.26287428004724234 1106 14-15 0.2630548589435193 1106 16-17 0.19359244386482288 1106 18-19 0.2795534001813138 1106 20-21 0.2529180650463374 1106 22-23 0.3164855147886172 1106 24-25 0.22983151624058706 1106 26-27 0.267004460928149 1106 28-29 0.2364442024031419 1106 30-31 0.14988659971324125 1106 32-33 0.10237819776200752 1106 34-35 0.09205968412867804 1106 36-37 0.12964718269070374 1106 38-39 0.5601827398540777 1106 40-41 0.49461287707056556 1106 42-43 0.46852771985367525 1106 44-45 0.30749344015693225 1106 46-47 0.22935515421200847 1106 48-49 0.35804064409821734 1106 50-51 0.27481435844885027 1106 52-53 0.3136569470304451 1106 54-55 0.3277959686873402 1106 56-57 0.4877698878969525 1106 58-59 0.42966518700195877 1106 60-61 0.5061969540655511 1106 62-63 0.36638411789003555 1106 64-65 0.365551217809319 1106 66-67 0.29664808976950496 1106 68-69 0.23843686633069439 1106 70-71 0.1584805090829633 1106 72-73 0.24873283264941648 1106 74-75 0.25261276145296563 1106 76-77 0.44777092244740047 1106 78-79 0.4302536790502707 1106 80-81 0.44635424404172497 1106 82-83 0.5050131328992933 1106 84-85 0.4646585976973654 1106 86-87 0.4567056071498605 1106 88-89 0.43243406379995264 1106 90-91 0.4151856010496964 1106 92-93 0.3098520086009593 1106 94-95 0.45605837603140387 1106 96-97 0.47654417645758684 1106 98-99 0.521565853257119 1106 100-101 0.31869876218035387 1107 1 0.2400380232472017 1107 2 0.16684956022312392 1107 3 0.1915467649790905 1107 4 0.19261268415511523 1107 5 0.29634120766449 1107 6 0.22158476255808068 1107 7 0.17829693772574018 1107 8 0.18084994746488547 1107 9 0.31479814356912783 1107 10-11 0.2736863109291505 1107 12-13 0.34356549336568065 1107 14-15 0.3365079313024921 1107 16-17 0.3731946038184617 1107 18-19 0.42465340053323075 1107 20-21 0.3597326437069839 1107 22-23 0.28262355216697443 1107 24-25 0.316197256112396 1107 26-27 0.4291373520964257 1107 28-29 0.45653200294087526 1107 30-31 0.46819954723527246 1107 32-33 0.5243781285515254 1107 34-35 0.4590085399268773 1107 36-37 0.3143459909800086 1107 38-39 0.7274121327256324 1107 40-41 0.6926894707886078 1107 42-43 0.46331369229617536 1107 44-45 0.5012370469850822 1107 46-47 0.5927634978291607 1107 48-49 0.5204734718040029 1107 50-51 0.5803133953673694 1107 52-53 0.5343044433704947 1107 54-55 0.6545443105683617 1107 56-57 0.5497119516832214 1107 58-59 0.5103149766724684 1107 60-61 0.4593869855269972 1107 62-63 0.4886294819606434 1107 64-65 0.5833398959125375 1107 66-67 0.4893995085844338 1107 68-69 0.5364902041529334 1107 70-71 0.526470674742356 1107 72-73 0.4509963674048123 1107 74-75 0.4676503228039621 1107 76-77 0.632691805479336 1107 78-79 0.6578003755986579 1107 80-81 0.612104116178287 1107 82-83 0.5085817251815037 1107 84-85 0.5073115382045046 1107 86-87 0.705365387177828 1107 88-89 0.6749411490766555 1107 90-91 0.4873417833055349 1107 92-93 0.6418472970092566 1107 94-95 0.7553922544461784 1107 96-97 0.8697859779478385 1107 98-99 1.0735833242121728 1107 100-101 0.7931699178312144 1108 1 0.18758555167837443 1108 2 0.20128479002297794 1108 3 0.2852617345138917 1108 4 0.205919458369074 1108 5 0.20706917463189 1108 6 0.2529548266525268 1108 7 0.2759322118221803 1108 8 0.2807509016495544 1108 9 0.41665402371521765 1108 10-11 0.498861858760435 1108 12-13 0.44305427457670277 1108 14-15 0.45538762877487216 1108 16-17 0.49544315852926246 1108 18-19 0.5116734549095554 1108 20-21 0.5217670492738833 1108 22-23 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-1.5168832711358462 2316 68-69 -1.55846171892399 2316 70-71 -1.197184290719008 2316 72-73 -1.0884814312193285 2316 74-75 -1.120593750196214 2316 76-77 -0.9945299311174018 2316 78-79 -1.0470511118366694 2316 80-81 -0.9636312510967144 2316 82-83 -1.0077059759385172 2316 84-85 -0.8382440114287881 2316 86-87 -0.8600128321638074 2316 88-89 -0.8970001210353473 2316 90-91 -1.1430264511023331 2316 92-93 -1.2051171587585507 2316 94-95 -0.9696223143183893 2316 96-97 -0.9440527493817399 2316 98-99 -1.1685055701251947 2316 100-101 -0.4809369458335766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10565.0 3 2696.0 4 2709.0 5 3113.0 6 3589.0 7 4124.0 8 5153.0 9 6315.0 10 7023.0 11 8831.0 12 10964.0 13 13827.0 14 14048.0 15 14063.0 16 17054.0 17 20942.0 18 18909.0 19 14814.0 20 15158.0 21 16283.0 22 17594.0 23 19829.0 24 22221.0 25 25665.0 26 30047.0 27 34814.0 28 40909.0 29 48557.0 30 57955.0 31 70836.0 32 87914.0 33 113882.0 34 154445.0 35 223007.0 36 350718.0 37 562680.0 38 531349.0 39 75921.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70028412282134 18.657710632064692 14.197266909202014 23.444738335911957 2 15.146006764639107 19.864348152628153 40.84183588550158 24.147809197231158 3 19.678127833250052 24.098297132563935 29.14698040999279 27.076594624193223 4 12.737593226628995 16.035878036225203 37.21508266194199 34.01144607520381 5 13.896699386571932 37.34072664754732 34.15206056830491 14.610513397575847 6 32.49422845992475 35.46452067906276 17.64134713743946 14.399903723573038 7 29.08165096187489 32.19111002126203 20.541067706542186 18.186171310320905 8 26.20031388769879 35.440298903636176 19.643515392684876 18.715871815980158 9 26.431565877584536 17.140655569891475 20.24035493928129 36.1874236132427 10-11 25.14393449039163 26.53616880338725 27.26139714630002 21.058499559921103 12-13 25.89414268605748 23.766575364175832 27.770285524618032 22.56899642514865 14-15 22.817994337446258 25.23918747381952 26.063878406312813 25.878939782421412 16-17 22.376349561553088 28.523819089843073 26.61117210384441 22.488659244759425 18-19 22.240246115241465 27.38749860038593 28.650562677568736 21.72169260680387 20-21 23.191308310158824 26.433620801359115 28.533471797649636 21.84159909083243 22-23 22.629311876187604 26.385509096736286 28.396312464449075 22.588866562627036 24-25 22.53432220793299 26.48808121070929 28.595817519793577 22.38177906156414 26-27 22.43590151139182 26.6637632844695 28.569243421483208 22.331091782655477 28-29 22.245369439751528 26.99441555976697 28.619615755357948 22.14059924512355 30-31 22.402766403787258 26.652045635566125 28.502204372847512 22.442983587799105 32-33 22.306425244501554 26.641698708713164 28.475046658318252 22.576829388467033 34-35 22.74701862047351 26.732126288087656 28.315532510098727 22.205322581340113 36-37 22.70970875971551 26.447798817633466 28.472376436695374 22.370115985955646 38-39 22.42732685133208 26.74537989604649 28.45334560286249 22.37394764975894 40-41 22.613183745229293 26.715589505586117 28.32657869704322 22.344648052141366 42-43 22.363490542208698 26.706294386868883 28.11724199618249 22.812973074739926 44-45 22.535864701175466 26.825601942463084 28.031765302213117 22.606768054148333 46-47 22.641831422944396 26.94421034194218 27.74525347018672 22.668704764926705 48-49 22.686997536258414 26.83553999753926 27.859973351978944 22.617489114223382 50-51 22.584602331579738 27.178426932913585 27.709475434880826 22.52749530062585 52-53 22.717923844956076 27.19745027010137 27.45127165771831 22.633354227224252 54-55 22.549775948225815 26.99927016278445 27.60057951925255 22.850374369737185 56-57 22.613107903255468 27.212591553007815 27.477646098196523 22.696654445540197 58-59 22.766061090876104 26.845783501516202 27.68137800558275 22.70677740202494 60-61 22.662969975909313 26.957384829217272 27.869248954661956 22.51039624021146 62-63 22.459588453779634 26.993551257227267 27.91086166036371 22.635998628629387 64-65 22.3678727266112 26.96431257358846 27.77505627325339 22.892758426546948 66-67 22.456523508759823 27.02593002872361 27.524870102271365 22.9926763602452 68-69 22.425171952843296 26.972913370583374 27.733902951552313 22.86801172502102 70-71 22.509218793640265 26.822764507020803 27.651353937337902 23.016662762001033 72-73 22.469175954158885 26.761725722681962 27.77927513015243 22.98982319300672 74-75 22.301582880306086 27.150761053893035 27.445920428888932 23.10173563691195 76-77 22.444449236648737 26.861319754309935 27.507235968035165 23.186995041006163 78-79 22.454518744957543 26.79359612829268 27.6395008794477 23.11238424730208 80-81 22.378694211036386 27.011938730876366 27.481954661808455 23.127412396278796 82-83 22.48970921790056 26.87593007068537 27.555050433832974 23.079310277581097 84-85 22.45426104358027 26.828283146492172 27.460062795431806 23.257393014495754 86-87 22.308466764639533 26.800804610137813 27.61608462695459 23.274643998268065 88-89 22.40354152109426 26.640303293190044 27.56392152273705 23.392233662978647 90-91 22.291646229298546 26.640918240086105 27.602535878014656 23.464899652600693 92-93 22.386871230940276 26.541364765166325 27.633565563092294 23.43819844080111 94-95 22.23028798331025 26.538754950360865 27.671356625375598 23.559600440953282 96-97 22.13119381884691 26.39525459348306 27.831563878473986 23.641987709196044 98-99 22.180447038027943 26.419764386710703 27.51040617858413 23.889382396677224 100-101 22.288409895583293 25.892399033845415 27.975051008161305 23.844140062409984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2993.0 1 1713.5 2 436.5 3 448.5 4 420.0 5 423.0 6 620.0 7 1080.0 8 1669.5 9 2053.5 10 1969.0 11 1958.5 12 2205.0 13 2397.0 14 2574.0 15 2719.5 16 2901.5 17 3093.5 18 3255.5 19 3402.5 20 3710.5 21 4203.5 22 4804.5 23 5762.5 24 7049.5 25 8876.0 26 11743.5 27 15274.0 28 18564.5 29 22419.0 30 27117.5 31 32286.0 32 37671.5 33 42571.0 34 47927.0 35 53446.0 36 59587.5 37 66002.0 38 70922.0 39 75200.5 40 79045.5 41 84078.0 42 88906.5 43 93848.0 44 98802.0 45 102527.5 46 104984.0 47 109108.5 48 118469.5 49 118944.5 50 110686.5 51 104685.5 52 100056.5 53 96690.5 54 92503.0 55 84482.5 56 74589.0 57 65712.5 58 57948.5 59 50055.0 60 41476.5 61 34145.0 62 27779.0 63 21298.0 64 15922.5 65 12198.0 66 9256.0 67 7147.0 68 5447.0 69 3900.0 70 2884.0 71 2193.0 72 1706.5 73 1387.0 74 1074.0 75 839.0 76 607.0 77 359.0 78 238.5 79 173.0 80 113.0 81 73.5 82 57.5 83 39.5 84 24.5 85 18.0 86 19.0 87 15.5 88 11.5 89 6.0 90 6.0 91 10.5 92 6.5 93 2.5 94 2.5 95 2.0 96 2.5 97 3.5 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2938905705628572 2 0.18439094613947557 3 0.18442827979935397 4 0.20503646005223725 5 0.21888724786712802 6 0.10897695318508385 7 0.1252917625519498 8 0.1383585435093939 9 0.14324925295346583 10-11 0.12915579634936541 12-13 0.11916904233189025 14-15 0.13464384435149193 16-17 0.10865961707611736 18-19 0.13826520935969788 20-21 0.16626545426850667 22-23 0.1705028246647064 24-25 0.19435903332701152 26-27 0.17378818673400662 28-29 0.17175350227063318 30-31 0.1612067433549819 32-33 0.17126816469221384 34-35 0.3555471098520542 36-37 0.4744361497348563 38-39 0.43975317970781186 40-41 0.4290010856628293 42-43 0.453547967032885 44-45 0.45705733106145574 46-47 0.4605853619199656 48-49 0.4735401418977745 50-51 0.4667267489699643 52-53 0.4740254794761938 54-55 0.4554706505166232 56-57 0.4171103149915551 58-59 0.4056862150687611 60-61 0.36118449249369433 62-63 0.4704787817877447 64-65 0.46812676121540475 66-67 0.47775884546403496 68-69 0.490452289822695 70-71 0.4828548900374382 72-73 0.47531349074199897 74-75 0.4804655358052199 76-77 0.4547426441489941 78-79 0.4411905256131307 80-81 0.43768116158456 82-83 0.45113994597072743 84-85 0.452185288447323 86-87 0.45410797193106117 88-89 0.46103336583850657 90-91 0.48098820704351763 92-93 0.4858042491678327 94-95 0.48382556519427694 96-97 0.4814735446219369 98-99 0.4787668542807521 100-101 0.4595026857834916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2678548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.90874223915724 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14064023239202 43.51299966902838 2 14.074065816432272 16.300228992414212 3 4.747429089550131 8.247529423363066 4 2.196344235247747 5.087501287496829 5 1.117989417273808 3.237068049550728 6 0.6601330119937531 2.2936483461062847 7 0.43443534925132676 1.7610323261561336 8 0.2886548467289435 1.3372511292247864 9 0.21981194929216596 1.1456130161382065 >10 1.033625384317355 10.533731404487986 >50 0.05720056038377722 2.2682245252128816 >100 0.027733206483777327 2.7854538686205244 >500 0.001355830457105905 0.5551718414199063 >1k 5.165068407682547E-4 0.7047231489273341 >5k 6.456335509603184E-5 0.22982297185295364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6147 0.2294900072725969 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4819 0.17991090695406614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3592 0.13410250628325496 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3177 0.11860903743371408 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.7333659878411736E-5 0.0 0.0 0.0 0.0 9 3.7333659878411736E-5 0.0 0.0 0.0 3.7333659878411736E-5 10-11 3.7333659878411736E-5 0.0 0.0 0.0 3.7333659878411736E-5 12-13 3.7333659878411736E-5 0.0 0.0 0.0 7.466731975682347E-5 14-15 3.7333659878411736E-5 0.0 0.0 3.7333659878411736E-5 7.466731975682347E-5 16-17 3.7333659878411736E-5 0.0 0.0 5.6000489817617604E-5 7.466731975682347E-5 18-19 3.7333659878411736E-5 0.0 0.0 7.466731975682347E-5 7.466731975682347E-5 20-21 3.7333659878411736E-5 0.0 0.0 1.1200097963523521E-4 1.4933463951364694E-4 22-23 3.7333659878411736E-5 0.0 0.0 1.6800146945285282E-4 1.4933463951364694E-4 24-25 3.7333659878411736E-5 0.0 0.0 2.4266878920967626E-4 1.4933463951364694E-4 26-27 3.7333659878411736E-5 0.0 0.0 6.90672707750617E-4 1.4933463951364694E-4 28-29 3.7333659878411736E-5 0.0 0.0 0.002240019592704704 1.4933463951364694E-4 30-31 3.7333659878411736E-5 0.0 0.0 0.007354730996047112 1.4933463951364694E-4 32-33 3.7333659878411736E-5 0.0 0.0 0.016781480115346074 1.4933463951364694E-4 34-35 3.7333659878411736E-5 0.0 0.0 0.03139760795774427 1.4933463951364694E-4 36-37 3.7333659878411736E-5 0.0 0.0 0.05743783572293646 1.4933463951364694E-4 38-39 3.7333659878411736E-5 0.0 0.0 0.09945686991608887 1.866682993920587E-4 40-41 3.7333659878411736E-5 0.0 0.0 0.14541460522641372 1.866682993920587E-4 42-43 3.7333659878411736E-5 0.0 0.0 0.19299635474144947 1.866682993920587E-4 44-45 3.7333659878411736E-5 0.0 0.0 0.25116219683201496 1.866682993920587E-4 46-47 3.7333659878411736E-5 0.0 0.0 0.32446683800327636 1.866682993920587E-4 48-49 3.7333659878411736E-5 0.0 0.0 0.40514487700052415 1.866682993920587E-4 50-51 3.7333659878411736E-5 0.0 0.0 0.49258030843576445 1.866682993920587E-4 52-53 3.7333659878411736E-5 0.0 0.0 0.5797170705919774 1.866682993920587E-4 54-55 3.7333659878411736E-5 0.0 0.0 0.6818432971893728 1.866682993920587E-4 56-57 3.7333659878411736E-5 0.0 0.0 0.8031590249642717 1.866682993920587E-4 58-59 3.7333659878411736E-5 0.0 0.0 0.9393895498605961 1.866682993920587E-4 60-61 3.7333659878411736E-5 0.0 0.0 1.0948469095943025 1.866682993920587E-4 62-63 3.7333659878411736E-5 0.0 0.0 1.2503416029878873 1.866682993920587E-4 64-65 3.7333659878411736E-5 0.0 0.0 1.4158043835690082 1.866682993920587E-4 66-67 3.7333659878411736E-5 0.0 0.0 1.6038726952065074 1.866682993920587E-4 68-69 3.7333659878411736E-5 0.0 0.0 1.8276506525177072 1.866682993920587E-4 70-71 3.7333659878411736E-5 0.0 0.0 2.071420784693797 1.866682993920587E-4 72-73 3.7333659878411736E-5 0.0 0.0 2.3249536689280905 1.866682993920587E-4 74-75 3.7333659878411736E-5 0.0 0.0 2.585486614389587 1.866682993920587E-4 76-77 3.7333659878411736E-5 0.0 0.0 2.8652277278585263 1.866682993920587E-4 78-79 3.7333659878411736E-5 0.0 0.0 3.196433291469856 1.866682993920587E-4 80-81 3.7333659878411736E-5 0.0 0.0 3.5585324586305713 1.866682993920587E-4 82-83 3.7333659878411736E-5 0.0 0.0 3.9322983945032908 2.2400195927047042E-4 84-85 3.7333659878411736E-5 0.0 0.0 4.313997733100172 2.2400195927047042E-4 86-87 3.7333659878411736E-5 0.0 0.0 4.72705734599492 2.2400195927047042E-4 88-89 3.7333659878411736E-5 0.0 0.0 5.176666611910632 2.4266878920967626E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13135 0.0 25.906801 1 GTACATG 10610 0.0 25.183596 1 GGTATCA 9490 0.0 24.705032 1 TACATGG 10840 0.0 24.19105 2 ACATGGG 11070 0.0 22.746038 3 ATCAACG 15085 0.0 22.35029 3 TCAACGC 15220 0.0 22.151217 4 CAACGCA 15340 0.0 21.946611 5 TATCAAC 15480 0.0 21.77998 2 AACGCAG 15690 0.0 21.534008 6 CATGGGG 5050 0.0 20.939007 4 TACCGTA 115 0.0037763466 20.602999 7 GAGTACT 7000 0.0 20.171206 12-13 ACGCAGA 17545 0.0 19.149223 7 CGCAGAG 17575 0.0 18.981724 8 AGAGTAC 13420 0.0 18.676891 10-11 AGTACTT 7495 0.0 18.522926 12-13 GTACCGT 205 1.623677E-5 18.492449 6 GTACTTT 7535 0.0 18.491629 14-15 GTGTACG 360 9.822543E-11 18.456537 1 >>END_MODULE