FastQCFastQC Report
Fri 27 May 2016
SRR939309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61750.2305353497491925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50360.1880123111476815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41190.15377734503917795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.13148915009176615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126250.025.0356831
GTACATG106700.025.0277141
TACATGG106650.024.1613962
ACATGGG107550.022.8561133
GGTATCA93100.022.8038351
ATCAACG148000.021.1624153
TCAACGC149650.020.9290854
CAACGCA149650.020.8973735
TATCAAC153300.020.5855522
AACGCAG154250.020.3357096
CATGGGA64000.019.0561944
GAGTACT72650.019.00888412-13
AGAGTAC133150.018.64041910-11
GTACTTT74300.018.49094214-15
AGTACTT76900.018.32860212-13
GTACCGT2603.3168908E-718.2519686
ACGCAGA172250.018.1555427
CGCAGAG173450.017.9478558
GAGTACA91600.017.2010631
AGTACAT79650.016.9205932
Produced by FastQC (version 0.11.5)