##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939309_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2678548 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3517267564367 34.0 31.0 34.0 31.0 34.0 2 32.557594637094425 34.0 31.0 34.0 31.0 34.0 3 32.654764447006364 34.0 31.0 34.0 31.0 34.0 4 36.159510301850105 37.0 35.0 37.0 35.0 37.0 5 36.10413365748906 37.0 35.0 37.0 35.0 37.0 6 36.12997937688628 37.0 36.0 37.0 35.0 37.0 7 36.07623048009593 37.0 35.0 37.0 35.0 37.0 8 36.04676190234411 37.0 35.0 37.0 35.0 37.0 9 37.764942050693136 39.0 38.0 39.0 35.0 39.0 10-11 37.68846311509071 39.0 37.0 39.0 35.0 39.0 12-13 37.61709347751095 39.0 37.0 39.0 35.0 39.0 14-15 38.93566271726323 40.0 38.0 41.0 35.0 41.0 16-17 38.938082125091654 40.0 38.0 41.0 35.5 41.0 18-19 39.02877361167319 40.0 38.5 41.0 35.0 41.0 20-21 39.00650053685803 40.0 38.5 41.0 35.0 41.0 22-23 38.931996178526575 40.0 38.0 41.0 35.0 41.0 24-25 38.87597104849344 40.0 38.0 41.0 35.0 41.0 26-27 38.729487767253005 40.0 38.0 41.0 34.5 41.0 28-29 38.57611194572581 40.0 38.0 41.0 34.0 41.0 30-31 38.40669123719269 40.0 38.0 41.0 34.0 41.0 32-33 38.38115426716266 40.0 38.0 41.0 34.0 41.0 34-35 38.30512240213728 40.0 38.0 41.0 34.0 41.0 36-37 38.1750468910768 40.0 38.0 41.0 33.5 41.0 38-39 38.05835064370696 40.0 38.0 41.0 33.0 41.0 40-41 37.89618871866399 40.0 37.5 41.0 33.0 41.0 42-43 37.73492597481919 40.0 37.0 41.0 33.0 41.0 44-45 37.51758415380273 40.0 37.0 41.0 32.5 41.0 46-47 37.25499076365254 40.0 37.0 41.0 31.5 41.0 48-49 36.98460527868084 40.0 36.5 41.0 31.0 41.0 50-51 36.76110284377954 39.5 36.0 41.0 31.0 41.0 52-53 36.52119879875216 39.0 36.0 41.0 30.0 41.0 54-55 36.28514889410233 39.0 35.0 40.5 30.0 41.0 56-57 35.98344326851712 39.0 35.0 40.0 29.0 41.0 58-59 35.68027696348918 38.0 35.0 40.0 28.5 41.0 60-61 35.34883526447911 38.0 34.5 40.0 28.0 41.0 62-63 35.01204999873066 38.0 34.0 40.0 28.0 41.0 64-65 34.7117417720347 37.0 34.0 40.0 27.5 41.0 66-67 34.29987720959266 36.5 34.0 39.0 26.0 41.0 68-69 33.833897693825165 36.0 33.0 39.0 26.0 40.0 70-71 33.353409571155716 35.5 33.0 38.5 26.0 40.0 72-73 32.85927562246411 35.0 32.0 37.5 25.0 40.0 74-75 32.3368968933915 35.0 32.0 37.0 23.5 39.0 76-77 30.36358616683367 33.5 29.5 35.5 20.0 37.5 78-79 31.331105882739454 34.0 31.0 36.0 21.5 38.0 80-81 31.34281073178453 34.0 31.0 36.0 22.0 37.0 82-83 30.992455427343472 34.0 31.0 35.0 20.5 37.0 84-85 30.546942037253018 34.0 31.0 35.0 19.0 36.5 86-87 29.959027054956643 34.0 30.0 35.0 15.5 36.0 88-89 29.38363546219818 34.0 29.0 35.0 7.0 35.5 90-91 28.915586541663618 34.0 29.0 35.0 4.5 35.0 92-93 28.557052179016395 34.0 29.0 35.0 2.0 35.0 94-95 28.058184135583907 33.0 28.5 35.0 2.0 35.0 96-97 27.35952127794611 33.0 27.0 35.0 2.0 35.0 98-99 26.396138691559756 33.0 25.5 34.0 2.0 35.0 100-101 22.11939341762776 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22085441118547777 1101 2 0.25118538761724807 1101 3 0.2785650886595903 1101 4 0.1605245420079342 1101 5 0.19484469927767378 1101 6 0.21558615497439604 1101 7 0.20912657609302698 1101 8 0.22591834034781755 1101 9 0.2357864436717847 1101 10-11 0.286790886552275 1101 12-13 0.2892206925463938 1101 14-15 0.31119397156802364 1101 16-17 0.30788817975086147 1101 18-19 0.37024867458186606 1101 20-21 0.38810656442157665 1101 22-23 0.35371497050561373 1101 24-25 0.3243878292994964 1101 26-27 0.3816072546616027 1101 28-29 0.2843313168833035 1101 30-31 0.45650166196059416 1101 32-33 0.4542162864406407 1101 34-35 0.5582087602494568 1101 36-37 0.5763530169972242 1101 38-39 0.520365941533754 1101 40-41 0.5032741597114452 1101 42-43 0.5868470857206489 1101 44-45 0.5359402366330883 1101 46-47 0.5407775899749083 1101 48-49 0.5639394544079508 1101 50-51 0.5033498919364945 1101 52-53 0.39868690937178286 1101 54-55 0.37920338432110867 1101 56-57 0.38289254632425695 1101 58-59 0.5951691075952255 1101 60-61 0.5250259368284702 1101 62-63 0.39578998519159114 1101 64-65 0.5266334156403119 1101 66-67 0.47585086304180635 1101 68-69 0.5500768505623981 1101 70-71 0.5101188252369226 1101 72-73 0.4424520668892953 1101 74-75 0.4261805205771907 1101 76-77 0.5148510652913849 1101 78-79 0.6720280511639025 1101 80-81 0.6409706222426053 1101 82-83 0.644511008564919 1101 84-85 0.7528578390260492 1101 86-87 0.5724703844441379 1101 88-89 0.7509561844194046 1101 90-91 0.8710646585319033 1101 92-93 0.9080434829293225 1101 94-95 0.6805095781315416 1101 96-97 0.5696686352530307 1101 98-99 0.4103833693475245 1101 100-101 0.5403880798063483 1102 1 0.1570506468557511 1102 2 0.1628151451440445 1102 3 0.1682991530833391 1102 4 0.1048808508255874 1102 5 0.17822911693080812 1102 6 0.16640719404671245 1102 7 0.16364559757431607 1102 8 0.17253103302819284 1102 9 0.19709869729537388 1102 10-11 0.19437133716773047 1102 12-13 0.2225297776003501 1102 14-15 0.2711805367752902 1102 16-17 0.19887928504110164 1102 18-19 0.22902700697115108 1102 20-21 0.2923099868793173 1102 22-23 0.2547893585720047 1102 24-25 0.2419207369940608 1102 26-27 0.18796599453698803 1102 28-29 0.2218816344590877 1102 30-31 0.2613510542680402 1102 32-33 0.31346868227607416 1102 34-35 0.266256481438333 1102 36-37 0.3959972142306256 1102 38-39 0.3501187600368212 1102 40-41 0.3881691109896579 1102 42-43 0.43442903867514815 1102 44-45 0.4195046474430342 1102 46-47 0.3187642256267722 1102 48-49 0.31180005890893625 1102 50-51 0.16887584717973425 1102 52-53 0.17578004864220986 1102 54-55 0.12494993181658742 1102 56-57 0.24009108653950761 1102 58-59 0.18577039543762908 1102 60-61 0.2205098984605982 1102 62-63 0.28703339718327214 1102 64-65 0.1966139139855798 1102 66-67 0.2213808652557887 1102 68-69 0.22912475576983127 1102 70-71 0.04698777095430984 1102 72-73 0.2370826783772273 1102 74-75 0.1187187475583329 1102 76-77 0.17463021426303627 1102 78-79 0.08728067414724094 1102 80-81 0.40392398360042847 1102 82-83 0.3692731501959585 1102 84-85 0.3879204556550029 1102 86-87 0.09223998026461544 1102 88-89 0.34199164105859126 1102 90-91 0.6762880182508191 1102 92-93 0.5531841708712193 1102 94-95 0.23032829109421016 1102 96-97 -0.27672941702548925 1102 98-99 -0.45561248017129685 1102 100-101 -0.48507926264932166 1103 1 0.12191291226216805 1103 2 0.16462684134992855 1103 3 0.17071870240443587 1103 4 0.09756483484328271 1103 5 0.1153913051736879 1103 6 0.14516048783860924 1103 7 0.16143126286450382 1103 8 0.1766546249885934 1103 9 0.1979615193840445 1103 10-11 0.14202921696614368 1103 12-13 0.16964876984964405 1103 14-15 0.25932040708815407 1103 16-17 0.15733403849496597 1103 18-19 0.161595061554344 1103 20-21 0.22312495270816157 1103 22-23 0.21062643056497876 1103 24-25 0.15670966340824322 1103 26-27 0.21056686929304647 1103 28-29 0.21425363560786792 1103 30-31 0.17119181197917044 1103 32-33 0.2583396095451178 1103 34-35 0.28640947698735175 1103 36-37 0.23204499329150252 1103 38-39 0.3476976171596462 1103 40-41 0.26894491075119475 1103 42-43 0.27467929072260233 1103 44-45 0.31403841365701624 1103 46-47 0.3643436479308235 1103 48-49 0.2652808811697085 1103 50-51 0.3181327365653388 1103 52-53 0.1498242071436735 1103 54-55 0.1922772337172063 1103 56-57 0.18276821826603395 1103 58-59 0.15272916093452693 1103 60-61 0.363827427258812 1103 62-63 0.34536144522225953 1103 64-65 0.34845882698272845 1103 66-67 0.20674042285628502 1103 68-69 0.15683866145893433 1103 70-71 0.10933335094321706 1103 72-73 0.2988112865688848 1103 74-75 0.19188041240658293 1103 76-77 0.30059506820654036 1103 78-79 0.11196556613778341 1103 80-81 0.3350128640139616 1103 82-83 0.3117099205464626 1103 84-85 0.23961491925162903 1103 86-87 0.17402083584842032 1103 88-89 0.41372255813595515 1103 90-91 0.5520021527037464 1103 92-93 0.41480038024040766 1103 94-95 0.10399895546191118 1103 96-97 0.003223151662972157 1103 98-99 0.11108961655897431 1103 100-101 0.17751037930695546 1104 1 0.08336648667493307 1104 2 0.06796699633540726 1104 3 0.07418414260554584 1104 4 0.04533163865890799 1104 5 0.14236649051910177 1104 6 0.03889510204707847 1104 7 0.07957041434097789 1104 8 0.09917517943448217 1104 9 0.124904386257505 1104 10-11 0.12007975855623698 1104 12-13 0.095322932151781 1104 14-15 0.09909595734593069 1104 16-17 0.07970450636620541 1104 18-19 0.0787273289917394 1104 20-21 0.09653625946875621 1104 22-23 0.11303333853607711 1104 24-25 0.16175226549524524 1104 26-27 0.17860737783851732 1104 28-29 0.16458034876812633 1104 30-31 0.10028268109038407 1104 32-33 -0.03738207932005366 1104 34-35 0.039074472502655055 1104 36-37 0.14600575498470647 1104 38-39 0.17713330186635545 1104 40-41 0.23972123931141454 1104 42-43 0.24780228807678384 1104 44-45 0.17468034441980507 1104 46-47 0.128608264624539 1104 48-49 0.15275225733319786 1104 50-51 0.19300860192822 1104 52-53 0.24067030678479284 1104 54-55 0.21382194417540035 1104 56-57 0.15524586891767456 1104 58-59 0.1528288777387985 1104 60-61 0.1263996708050854 1104 62-63 0.23088118454719364 1104 64-65 0.1880569324657202 1104 66-67 0.31077061812735707 1104 68-69 0.284630604136602 1104 70-71 0.13169599488059447 1104 72-73 0.08220976821434789 1104 74-75 0.0864598069261433 1104 76-77 0.22549604174936988 1104 78-79 0.2572509044947999 1104 80-81 0.3477651091761622 1104 82-83 0.33814995904226564 1104 84-85 0.3612914093019377 1104 86-87 0.3551525755538094 1104 88-89 0.45396263529119096 1104 90-91 0.5674316662186989 1104 92-93 0.29034690503307203 1104 94-95 0.3613948741298145 1104 96-97 0.24595175115835488 1104 98-99 0.2181064579601646 1104 100-101 0.25706751396759486 1105 1 0.11324792396340655 1105 2 0.14746246015065623 1105 3 0.16910289276086132 1105 4 0.08735060959640606 1105 5 0.07856573215924811 1105 6 0.09876835157935204 1105 7 0.12256016851142704 1105 8 0.04595670060145807 1105 9 0.11889088759410527 1105 10-11 0.20363957032498092 1105 12-13 0.16994366347299916 1105 14-15 0.15805522479187317 1105 16-17 0.15341779096028318 1105 18-19 0.12775656189548812 1105 20-21 0.1567209471060238 1105 22-23 0.18731294024385647 1105 24-25 0.2493076829207368 1105 26-27 0.2233563538848955 1105 28-29 0.3055319099766862 1105 30-31 0.20663968146055112 1105 32-33 0.08643828834473766 1105 34-35 0.2493613537937449 1105 36-37 0.11263992235897291 1105 38-39 0.30363042356507464 1105 40-41 0.41087681022909806 1105 42-43 0.33791440256723604 1105 44-45 0.23950626665580899 1105 46-47 0.2225643805750508 1105 48-49 0.24200234987711156 1105 50-51 0.2609106612602403 1105 52-53 0.3316810735066795 1105 54-55 0.22712737071812228 1105 56-57 0.21716363699407282 1105 58-59 0.18118555924799296 1105 60-61 0.1633179955972679 1105 62-63 0.14883023391868733 1105 64-65 0.18672355782246086 1105 66-67 0.1872928323780556 1105 68-69 0.23926101101677233 1105 70-71 0.18463192028260522 1105 72-73 0.2898067440696579 1105 74-75 0.12864095159189048 1105 76-77 0.2099810990136426 1105 78-79 0.3382814515705235 1105 80-81 0.25926835797583436 1105 82-83 0.3416116823309636 1105 84-85 0.37520075144461273 1105 86-87 0.5594803388247058 1105 88-89 0.539926491043385 1105 90-91 0.5412721563351361 1105 92-93 0.584470261632287 1105 94-95 0.5539485826799044 1105 96-97 0.6316665532467027 1105 98-99 0.5939789284130796 1105 100-101 0.7359955795109592 1106 1 0.1452498862106637 1106 2 0.09700180821482718 1106 3 0.14984007365644914 1106 4 0.11864918826857007 1106 5 0.0704561513063453 1106 6 0.10270341062667399 1106 7 0.1232240499179511 1106 8 0.06497087039678462 1106 9 0.04756788494547237 1106 10-11 0.11434606660970559 1106 12-13 0.11626725941970761 1106 14-15 0.15818085087445155 1106 16-17 0.12995801955534603 1106 18-19 0.16922442267085813 1106 20-21 0.1862677270898132 1106 22-23 0.1805830262161905 1106 24-25 0.15832262897960447 1106 26-27 0.27070421962712743 1106 28-29 0.16816273602980658 1106 30-31 0.16997064517413918 1106 32-33 0.17828481108979588 1106 34-35 0.33839150971860477 1106 36-37 0.27656473533993164 1106 38-39 0.2111439810002267 1106 40-41 0.35352241744249824 1106 42-43 0.300527820106538 1106 44-45 0.35075218189426494 1106 46-47 0.24873923707728807 1106 48-49 0.2915574375650323 1106 50-51 0.3380548749504442 1106 52-53 0.4117544189284885 1106 54-55 0.24027286562593986 1106 56-57 0.37910622197004784 1106 58-59 0.4063439392120003 1106 60-61 0.49421441769601415 1106 62-63 0.4466367764883259 1106 64-65 0.5404989316500135 1106 66-67 0.6029381714972786 1106 68-69 0.5503683777578132 1106 70-71 0.4809647104356074 1106 72-73 0.6400669607773395 1106 74-75 0.6228826684235003 1106 76-77 0.547530344667674 1106 78-79 0.6589009558121823 1106 80-81 0.6015846579638762 1106 82-83 0.7277065668460665 1106 84-85 0.6117183780624593 1106 86-87 0.7030708746563121 1106 88-89 0.9313157379100474 1106 90-91 0.9823967769497273 1106 92-93 1.0686990973885493 1106 94-95 1.363769219868253 1106 96-97 1.2372460508250498 1106 98-99 1.4758199805671168 1106 100-101 1.2259765740683868 1107 1 0.1335622589337504 1107 2 0.10203056575760172 1107 3 0.07523718347354702 1107 4 0.06744178790820854 1107 5 0.08502877070029768 1107 6 0.12246828266939502 1107 7 0.14291575240202548 1107 8 0.1780574292515027 1107 9 0.1828581056213423 1107 10-11 0.27322225257241683 1107 12-13 0.22016854591357315 1107 14-15 0.19962473407530723 1107 16-17 0.196856771068596 1107 18-19 0.22406134106817177 1107 20-21 0.19625060629287816 1107 22-23 0.2198502250215526 1107 24-25 0.18385777852117258 1107 26-27 0.2648997034670657 1107 28-29 0.28215203714468373 1107 30-31 0.28557290249167977 1107 32-33 0.2958988646892067 1107 34-35 0.3240685319551062 1107 36-37 0.3723246987170299 1107 38-39 0.36441184493006773 1107 40-41 0.3606467716734869 1107 42-43 0.43300658480497134 1107 44-45 0.39141451449873443 1107 46-47 0.3615128091125044 1107 48-49 0.30247986375615454 1107 50-51 0.40500656075361974 1107 52-53 0.36711109046726165 1107 54-55 0.39174968832510615 1107 56-57 0.38717371568245795 1107 58-59 0.356595531053145 1107 60-61 0.4231090030900191 1107 62-63 0.2958448736457697 1107 64-65 0.30539912778548484 1107 66-67 0.38697513986691945 1107 68-69 0.5206907167906465 1107 70-71 0.5477525345518259 1107 72-73 0.6302297608458431 1107 74-75 0.4051440652787619 1107 76-77 0.3971985742400257 1107 78-79 0.5850961856848365 1107 80-81 0.5279797477732799 1107 82-83 0.6401778122359438 1107 84-85 0.4369130032231716 1107 86-87 0.46317472557458217 1107 88-89 0.8855863767110854 1107 90-91 0.9606616055892623 1107 92-93 0.8840714058045442 1107 94-95 0.9043471655368513 1107 96-97 0.8558384356775726 1107 98-99 0.8649722782378895 1107 100-101 0.7658879289281337 1108 1 0.1070117088424496 1108 2 0.11162524067488278 1108 3 0.09528054214934656 1108 4 0.1015334931460572 1108 5 0.07384557718749107 1108 6 0.054407478821921984 1108 7 0.14245950884311043 1108 8 0.10540339690595601 1108 9 0.16982366502396928 1108 10-11 0.117455333987742 1108 12-13 0.08209469604285147 1108 14-15 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-0.5787520764729095 2316 84-85 -0.5778933921091429 2316 86-87 -0.6176410541741255 2316 88-89 -0.9406757757705755 2316 90-91 -1.0585771586952362 2316 92-93 -0.7875215464667633 2316 94-95 -0.5184574815437095 2316 96-97 -0.36029140754830635 2316 98-99 -0.20605089320448755 2316 100-101 0.14707121464648054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 21.0 5 59.0 6 118.0 7 239.0 8 582.0 9 1105.0 10 1978.0 11 3130.0 12 4403.0 13 6245.0 14 7814.0 15 10243.0 16 13256.0 17 18197.0 18 17970.0 19 13141.0 20 12497.0 21 13510.0 22 14612.0 23 16731.0 24 19119.0 25 22890.0 26 27765.0 27 33498.0 28 41269.0 29 50443.0 30 61180.0 31 75973.0 32 94244.0 33 121774.0 34 166801.0 35 240797.0 36 387833.0 37 641220.0 38 504503.0 39 33378.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71279035588781 19.261901151220023 14.671174940885068 23.3541335520071 2 15.521095757850894 20.431442707018878 39.95228011594341 24.09518141918681 3 19.707468374656717 24.14793388059501 29.47697782529938 26.667619919448892 4 13.127933492324948 16.573718298122714 36.764097563306684 33.53425064624565 5 13.818307530796536 37.550829777924456 34.15917877895039 14.47168391232862 6 32.50690299371152 35.89993533810109 17.238033441999175 14.355128226188219 7 29.114617322519514 32.24209534419394 20.343409937025584 18.29987739626096 8 26.17649562374839 35.756238081229085 19.42970594516133 18.637560349861193 9 26.246234900401262 17.071039981363036 20.121647997347818 36.56107712088789 10-11 25.17046549100483 26.722631067279735 27.084767568100325 21.02213587361511 12-13 25.84835142024709 23.82301903867319 27.687146170238503 22.641483370841218 14-15 22.657080978321133 25.389517369191122 25.98567037532476 25.967731277162986 16-17 22.309453328091628 28.695655095711547 26.56038770707821 22.434503869118622 18-19 22.23134823308089 27.53506425966471 28.514177371944076 21.719410135310326 20-21 23.064781017469546 26.496697649882567 28.55591109657587 21.88261023607202 22-23 22.62122986035718 26.43878325122417 28.380506901500365 22.559479986918284 24-25 22.498831456445807 26.62298752906425 28.582538001932388 22.29564301255755 26-27 22.396603827190475 26.763490395623446 28.590882100334714 22.249023676851365 28-29 22.22928243212367 27.07438881065413 28.584946022994544 22.11138273422765 30-31 22.43224687405266 26.652275785238867 28.48003843873621 22.43543890197226 32-33 22.256480003345096 26.781244913288845 28.452915534834545 22.509359548531517 34-35 22.71612589497303 26.79375882606046 28.29754685687245 22.192568422094062 36-37 22.714003078748533 26.500367202180737 28.457646299359947 22.327983419710787 38-39 22.43541340182996 26.85439769421248 28.427268849560996 22.282920054396573 40-41 22.61758933234968 26.7818774494083 28.306992879845193 22.293540338396827 42-43 22.3804241079887 26.772985800657413 28.1240579054369 22.72253218591699 44-45 22.55770511259896 26.87895007318433 28.004930352149078 22.55841446206763 46-47 22.66744544797472 27.051506340758262 27.705051699729406 22.57599651153761 48-49 22.700327062687357 26.933139098056646 27.791629651767185 22.574904187488812 50-51 22.619927404684816 27.20530436738568 27.68334330453352 22.491424923395982 52-53 22.805712502230687 27.218666395872987 27.399977450452596 22.57564365144373 54-55 22.563250686566004 27.038996501089397 27.575835863311017 22.82191694903358 56-57 22.65672834251751 27.246501799501218 27.435356092170306 22.66141376581097 58-59 22.78340181109844 26.92828221923961 27.589159543547936 22.699156426114016 60-61 22.67677096705487 27.066791685512513 27.768632004986316 22.487805342446293 62-63 22.475353871587007 27.12433087898738 27.77737954701749 22.622935702408125 64-65 22.392592400393273 27.05289977232055 27.673964219027 22.88054360825918 66-67 22.459511923586604 27.100051502024147 27.431146609779862 23.00928996460939 68-69 22.407663838841202 27.02954003003919 27.671074231445125 22.891721899674483 70-71 22.56422557374846 27.00233988756966 27.489413572094577 22.9440209665873 72-73 22.521922325080602 26.860298938081378 27.60447078043776 23.013307956400258 74-75 22.292548480099754 27.275846330893888 27.269182271361625 23.162422917644733 76-77 22.398003566450214 26.982883434572408 27.408645272956218 23.21046772602116 78-79 22.44432468615873 26.906843215351927 27.50571345957234 23.14311863891701 80-81 22.385617407370834 27.09927988849164 27.366738677195272 23.148364026942254 82-83 22.451809711828947 27.004070862273146 27.402663681965006 23.141455743932905 84-85 22.345782117774256 26.94821970709504 27.357769955961214 23.34822821916949 86-87 22.224749075383528 26.916953444775654 27.432088900715474 23.426208579125344 88-89 22.28508622857308 26.809552164529986 27.37216170447813 23.533199902418808 90-91 22.19320010070597 26.793460101810368 27.390376964164208 23.62296283331945 92-93 22.233407801540235 26.727428442574112 27.387692884353758 23.65147087153189 94-95 22.07634509443176 26.716303011930343 27.45069343539858 23.756658458239315 96-97 21.90780228691067 26.598403314034318 27.609006820112985 23.884787578942024 98-99 21.946108438329684 26.61769232592838 27.32150713885577 24.114692096886163 100-101 22.051789407662383 26.052775019226893 27.880599132363155 24.014836440747573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 355.0 1 249.0 2 171.5 3 213.5 4 208.5 5 225.0 6 403.5 7 849.5 8 1325.0 9 1568.0 10 1562.5 11 1494.5 12 1561.5 13 1802.5 14 2044.0 15 2168.0 16 2341.5 17 2494.5 18 2635.0 19 2879.0 20 3306.0 21 3949.5 22 4696.5 23 5799.0 24 7098.0 25 8850.5 26 11684.0 27 15167.0 28 18572.5 29 22274.0 30 26798.0 31 32297.5 32 38077.0 33 43080.0 34 48156.0 35 53495.5 36 59977.0 37 66418.5 38 71010.0 39 75066.0 40 79094.5 41 84516.5 42 89886.5 43 94685.0 44 99583.0 45 103559.0 46 105944.0 47 110254.0 48 119126.5 49 119506.0 50 111641.5 51 105815.0 52 101000.0 53 97690.0 54 93444.0 55 85085.5 56 74956.0 57 65889.0 58 57840.0 59 49828.5 60 41595.0 61 33984.5 62 27463.5 63 21190.0 64 15722.0 65 11768.5 66 8861.0 67 6998.0 68 5208.0 69 3598.5 70 2712.0 71 2002.0 72 1518.5 73 1289.5 74 1055.0 75 802.5 76 536.0 77 296.0 78 172.0 79 101.5 80 66.0 81 47.5 82 24.5 83 13.0 84 11.5 85 8.5 86 4.0 87 2.5 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.295047914019088 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001530680055014881 16-17 0.0011573434562307638 18-19 5.600048981761761E-5 20-21 3.7333659878411736E-5 22-23 0.0 24-25 0.0 26-27 1.306678095744411E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001698681524467734 36-37 0.006981394397262995 38-39 0.0026320230214280276 40-41 0.0035466976884491147 42-43 3.1733610896649976E-4 44-45 0.00140001224544044 46-47 1.4933463951364694E-4 48-49 3.546697688449115E-4 50-51 1.8666829939205868E-5 52-53 7.466731975682347E-5 54-55 0.0 56-57 0.0010453424765955288 58-59 0.0023520205723399396 60-61 6.90672707750617E-4 62-63 0.001269344435865999 64-65 5.600048981761761E-5 66-67 4.666707484801467E-4 68-69 0.0017546820142853516 70-71 1.8666829939205868E-5 72-73 0.0 74-75 1.8666829939205868E-5 76-77 0.26073828059082754 78-79 1.6800146945285282E-4 80-81 1.6800146945285282E-4 82-83 0.0 84-85 0.0 86-87 0.10617692869420298 88-89 0.3733739324439958 90-91 0.27647441822957813 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.333414969602935E-5 100-101 2.986692790272939E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2678548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.36427357023902 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78200083370352 41.58668879550469 2 14.657307294293581 16.522969562772467 3 5.073541572532532 8.57899455492613 4 2.3215895688604187 5.2341883830824765 5 1.2516748773885746 3.5274872605062506 6 0.7293111867841978 2.4664257149844127 7 0.4589370876223483 1.810735889079236 8 0.32381233649795205 1.460115769583109 9 0.22889795577058863 1.1611500298849737 >10 1.0775794133946957 10.735195698031255 >50 0.06464354685719548 2.4785815952865407 >100 0.02884886214538992 2.9270054653210695 >500 0.0011265318047833759 0.4708426040806443 >1k 5.963991907506509E-4 0.6208802639596326 >5k 1.3253315350014464E-4 0.418738412997139 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6175 0.2305353497491925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5036 0.1880123111476815 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4119 0.15377734503917795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.13148915009176615 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.6000489817617604E-5 14-15 0.0 0.0 0.0 0.0 7.466731975682347E-5 16-17 0.0 0.0 0.0 0.0 7.466731975682347E-5 18-19 0.0 0.0 0.0 0.0 7.466731975682347E-5 20-21 0.0 0.0 0.0 3.7333659878411736E-5 1.1200097963523521E-4 22-23 0.0 0.0 0.0 9.333414969602935E-5 1.1200097963523521E-4 24-25 0.0 0.0 0.0 1.6800146945285282E-4 1.1200097963523521E-4 26-27 0.0 0.0 0.0 6.346722179329995E-4 1.1200097963523521E-4 28-29 0.0 0.0 0.0 0.002277353252583116 1.4933463951364694E-4 30-31 0.0 0.0 0.0 0.007522732465499966 1.866682993920587E-4 32-33 0.0 0.0 0.0 0.017061482564434162 1.866682993920587E-4 34-35 0.0 0.0 0.0 0.03178961138646759 1.866682993920587E-4 36-37 0.0 0.0 0.0 0.058109841600747864 1.866682993920587E-4 38-39 0.0 0.0 0.0 0.10055821288250202 1.866682993920587E-4 40-41 0.0 0.0 0.0 0.14718795407063828 1.866682993920587E-4 42-43 0.0 0.0 0.0 0.1957030450826343 1.866682993920587E-4 44-45 0.0 0.0 0.0 0.2541115559624095 2.0533512933126457E-4 46-47 0.0 0.0 0.0 0.3280695361815431 2.2400195927047042E-4 48-49 0.0 0.0 0.0 0.4094942483763591 2.2400195927047042E-4 50-51 0.0 0.0 0.0 0.49762035251935005 2.6133561914888214E-4 52-53 0.0 0.0 0.0 0.5852237854240432 2.6133561914888214E-4 54-55 0.0 0.0 0.0 0.6882273530285812 2.6133561914888214E-4 56-57 0.0 0.0 0.0 0.8103270876609268 3.1733610896649976E-4 58-59 0.0 0.0 0.0 0.9477336228434212 3.733365987841174E-4 60-61 0.0 0.0 0.0 1.1041616577339664 3.733365987841174E-4 62-63 0.0 0.0 0.0 1.2612616985023228 3.733365987841174E-4 64-65 0.0 0.0 0.0 1.4284791610977292 3.733365987841174E-4 66-67 0.0 0.0 0.0 1.6183208215794527 3.733365987841174E-4 68-69 0.0 0.0 0.0 1.8437414599253028 3.733365987841174E-4 70-71 0.0 0.0 0.0 2.0887249360474405 3.733365987841174E-4 72-73 0.0 0.0 0.0 2.343116494458938 3.9200342872332326E-4 74-75 0.0 0.0 0.0 2.6042841121383677 4.2933708860173496E-4 76-77 0.0 0.0 0.0 2.8849959007641455 4.4800391854094083E-4 78-79 0.0 0.0 0.0 3.2187588200771464 4.4800391854094083E-4 80-81 0.0 0.0 0.0 3.5823139999731195 4.853375784193525E-4 82-83 0.0 0.0 0.0 3.9585812910576923 5.226712382977643E-4 84-85 0.0 0.0 0.0 4.342707317546671 5.226712382977643E-4 86-87 0.0 0.0 0.0 4.756662938278501 5.226712382977643E-4 88-89 0.0 0.0 0.0 5.208381555977343 5.226712382977643E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12625 0.0 25.035683 1 GTACATG 10670 0.0 25.027714 1 TACATGG 10665 0.0 24.161396 2 ACATGGG 10755 0.0 22.856113 3 GGTATCA 9310 0.0 22.803835 1 ATCAACG 14800 0.0 21.162415 3 TCAACGC 14965 0.0 20.929085 4 CAACGCA 14965 0.0 20.897373 5 TATCAAC 15330 0.0 20.585552 2 AACGCAG 15425 0.0 20.335709 6 CATGGGA 6400 0.0 19.056194 4 GAGTACT 7265 0.0 19.008884 12-13 AGAGTAC 13315 0.0 18.640419 10-11 GTACTTT 7430 0.0 18.490942 14-15 AGTACTT 7690 0.0 18.328602 12-13 GTACCGT 260 3.3168908E-7 18.251968 6 ACGCAGA 17225 0.0 18.155542 7 CGCAGAG 17345 0.0 17.947855 8 GAGTACA 9160 0.0 17.201063 1 AGTACAT 7965 0.0 16.920593 2 >>END_MODULE