FastQCFastQC Report
Fri 27 May 2016
SRR939308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729622
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63550.23281611886187903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.1836151672282829No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37040.1356964444161133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.12159192738042117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107200.027.3836141
TACATGG107650.026.429252
GTATCAA137950.025.0672511
ACATGGG111750.024.6957993
GGTATCA100300.023.3476431
ATCAACG154750.022.0315913
TCAACGC155750.021.8292454
CAACGCA156750.021.7504865
TATCAAC158150.021.5879272
AACGCAG161250.021.131486
GAGTACT72600.020.72601712-13
CATGGGA66800.020.3729114
CATGGGG51550.019.4089244
GTACTTT77850.019.26743114-15
ACGCAGA178700.018.9887857
CGCAGAG179950.018.8568848
GTGTAGC18500.018.4866331
AGAGTAC133000.018.17302710-11
AGTACTT77800.018.06150212-13
AGTACAT78550.017.3254032
Produced by FastQC (version 0.11.5)