##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939308_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2729622 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.809664488343074 33.0 31.0 34.0 30.0 34.0 2 31.892331245864813 33.0 31.0 34.0 30.0 34.0 3 31.95250807620982 34.0 31.0 34.0 30.0 34.0 4 35.441246443646776 37.0 35.0 37.0 33.0 37.0 5 35.48471839690624 37.0 35.0 37.0 33.0 37.0 6 35.570870252364614 37.0 35.0 37.0 33.0 37.0 7 35.485167909695924 37.0 35.0 37.0 33.0 37.0 8 35.461464261351935 37.0 35.0 37.0 33.0 37.0 9 37.089299544039434 39.0 37.0 39.0 33.0 39.0 10-11 36.99315106633812 39.0 37.0 39.0 33.0 39.0 12-13 36.891479662751834 39.0 37.0 39.0 33.0 39.0 14-15 38.13294734582297 40.0 38.0 41.0 33.0 41.0 16-17 38.070284456968764 40.0 38.0 41.0 33.0 41.0 18-19 37.99998186562095 40.0 38.0 41.0 33.0 41.0 20-21 37.89111844057529 40.0 38.0 41.0 32.5 41.0 22-23 37.81880971064858 40.0 38.0 41.0 32.0 41.0 24-25 37.85217971572621 40.0 38.0 41.0 32.0 41.0 26-27 37.778494787923016 40.0 38.0 41.0 32.0 41.0 28-29 37.64943497671106 40.0 38.0 41.0 32.0 41.0 30-31 37.486692296589055 40.0 37.0 41.0 32.0 41.0 32-33 37.30204438563288 40.0 37.0 41.0 31.0 41.0 34-35 37.0103164467461 40.0 36.5 41.0 30.5 41.0 36-37 36.776298879478546 40.0 36.0 41.0 30.0 41.0 38-39 36.47211335488943 39.5 35.5 41.0 30.0 41.0 40-41 36.35774495516229 39.0 36.0 41.0 30.0 41.0 42-43 36.20790003157947 39.0 35.0 41.0 29.5 41.0 44-45 36.20004399876613 39.0 35.5 41.0 29.0 41.0 46-47 36.05249334889592 39.0 35.5 41.0 28.5 41.0 48-49 35.88115314135071 39.0 35.0 41.0 27.5 41.0 50-51 34.7574537793145 38.0 34.0 40.0 25.5 40.5 52-53 34.876481432227614 38.5 34.5 39.5 26.0 40.5 54-55 35.38979737853813 39.0 35.0 40.5 26.5 41.0 56-57 35.29296528969945 39.0 35.0 41.0 26.0 41.0 58-59 35.10176445676361 39.0 35.0 41.0 26.0 41.0 60-61 34.820303507225546 38.0 34.0 40.0 26.0 41.0 62-63 34.46880080831705 38.0 34.0 40.0 25.0 41.0 64-65 34.10031150833339 37.0 34.0 40.0 23.5 41.0 66-67 33.7445164568574 37.0 33.5 39.5 23.0 41.0 68-69 33.328329343769944 36.0 33.0 39.0 22.0 41.0 70-71 32.911276726228024 36.0 33.0 39.0 21.5 40.5 72-73 32.470342047360404 35.0 32.0 38.5 20.5 40.0 74-75 32.014942728333814 35.0 32.0 37.5 19.0 39.5 76-77 31.561036839533095 35.0 31.5 37.0 18.0 39.0 78-79 31.125382928478743 35.0 31.0 36.0 14.5 39.0 80-81 30.66480468724241 35.0 31.0 36.0 9.5 37.5 82-83 30.22928431116103 34.0 30.5 35.5 7.0 37.0 84-85 29.80585663509453 34.0 30.0 35.0 2.0 37.0 86-87 29.469426902332998 34.0 30.0 35.0 2.0 36.0 88-89 29.067860311794085 34.0 29.0 35.0 2.0 36.0 90-91 28.67158401419684 34.0 29.0 35.0 2.0 35.5 92-93 28.26383909566966 34.0 29.0 35.0 2.0 35.0 94-95 27.79698837421445 34.0 28.0 35.0 2.0 35.0 96-97 27.229804712886985 34.0 26.5 35.0 2.0 35.0 98-99 26.506663193658316 33.5 25.0 35.0 2.0 35.0 100-101 24.05496896639901 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3338592250569121 1101 2 0.24670649417364743 1101 3 0.39332184759948774 1101 4 0.360253031250366 1101 5 0.3716590882515902 1101 6 0.42119590927276107 1101 7 0.3866439251533862 1101 8 0.315791955180849 1101 9 0.3560195004427129 1101 10-11 0.46023515232808876 1101 12-13 0.4505815473393042 1101 14-15 0.4768809435797934 1101 16-17 0.4090242193609015 1101 18-19 0.5472283569034673 1101 20-21 0.4960841501738571 1101 22-23 0.5662043994591102 1101 24-25 0.47539210724128367 1101 26-27 0.47883948824210876 1101 28-29 0.5259531137341398 1101 30-31 0.557335484976214 1101 32-33 0.5337527866971072 1101 34-35 0.4374507235403797 1101 36-37 0.32023018732613906 1101 38-39 0.4571293580063198 1101 40-41 0.4272629423041323 1101 42-43 0.21914078082883748 1101 44-45 0.34444496640325184 1101 46-47 0.40234908516394086 1101 48-49 0.40055784646661863 1101 50-51 0.3571873986471985 1101 52-53 0.41286214042641944 1101 54-55 0.355759099493163 1101 56-57 0.3161067263532189 1101 58-59 0.24611730062346027 1101 60-61 0.31005321985103507 1101 62-63 0.2770873969189296 1101 64-65 0.32241355953151896 1101 66-67 0.2975788620561559 1101 68-69 0.2299560828895011 1101 70-71 0.3052789060521306 1101 72-73 0.36144992069170456 1101 74-75 0.27287757276103974 1101 76-77 0.2726298460415677 1101 78-79 0.27510112462462644 1101 80-81 0.22202932871357817 1101 82-83 0.13913682473079447 1101 84-85 0.09868950603414461 1101 86-87 0.2207820626134307 1101 88-89 0.154153624026236 1101 90-91 0.2289110231078091 1101 92-93 0.29275032073441665 1101 94-95 0.12411148564488528 1101 96-97 -0.020640708001202768 1101 98-99 -0.256912744148984 1101 100-101 -0.41096256842695666 1102 1 0.24927542167411332 1102 2 0.30418256981947067 1102 3 0.25347065603955343 1102 4 0.2747284807920636 1102 5 0.14144049624698596 1102 6 0.20104094231557212 1102 7 0.2407805784765955 1102 8 0.2465119961339326 1102 9 0.2893134268084836 1102 10-11 0.288813782839334 1102 12-13 0.32487267267573827 1102 14-15 0.44672188626422127 1102 16-17 0.38632060913352007 1102 18-19 0.30796651987649426 1102 20-21 0.3357003847603295 1102 22-23 0.33449761699441183 1102 24-25 0.3189688658037255 1102 26-27 0.32574942929781514 1102 28-29 0.3436899965699425 1102 30-31 0.3367585782841047 1102 32-33 0.3285442438227619 1102 34-35 0.19051787364945483 1102 36-37 0.2885710962483259 1102 38-39 0.3490772559615962 1102 40-41 0.21857565037946358 1102 42-43 0.16912483656236077 1102 44-45 0.2775130745824512 1102 46-47 0.27897678795574166 1102 48-49 0.37483605023010824 1102 50-51 0.2704497051638768 1102 52-53 0.3331613128447515 1102 54-55 0.31566695860240657 1102 56-57 0.2991668270460366 1102 58-59 0.2774991346949065 1102 60-61 0.3385178011302017 1102 62-63 0.2520872466680828 1102 64-65 0.27679563435785326 1102 66-67 0.355841633466639 1102 68-69 0.2939484494393483 1102 70-71 0.25558317285803867 1102 72-73 0.31037055395130864 1102 74-75 0.04865001237168798 1102 76-77 0.08222940808686019 1102 78-79 0.13483462204366958 1102 80-81 0.06017999885003533 1102 82-83 -0.183064629485294 1102 84-85 -0.10268887757076683 1102 86-87 -0.23186567551029924 1102 88-89 -0.08908225955304161 1102 90-91 -0.1610073394195588 1102 92-93 -0.13452632430909262 1102 94-95 -0.2928395675782056 1102 96-97 -0.37375379951669174 1102 98-99 -0.4186927818566417 1102 100-101 -0.4960398132718531 1103 1 0.3115050081215074 1103 2 0.2684910082965679 1103 3 0.31425521128029743 1103 4 0.2891426368042289 1103 5 0.2696932869234985 1103 6 0.22265465103063775 1103 7 0.3461950254671535 1103 8 0.36719104489409204 1103 9 0.45662997981135334 1103 10-11 0.46106598325528125 1103 12-13 0.35321754884384404 1103 14-15 0.5064508231136102 1103 16-17 0.5804399566361056 1103 18-19 0.5500194537240688 1103 20-21 0.5536732998967082 1103 22-23 0.5747248430915377 1103 24-25 0.5415319563886527 1103 26-27 0.4302553905489006 1103 28-29 0.526816779072071 1103 30-31 0.46647871658361595 1103 32-33 0.5225160261847606 1103 34-35 0.6222974538863184 1103 36-37 0.5738345804671567 1103 38-39 0.7107787910196492 1103 40-41 0.6158184138922209 1103 42-43 0.5222735618106071 1103 44-45 0.6137139520847015 1103 46-47 0.5190654446770182 1103 48-49 0.5332975703275125 1103 50-51 0.3433936798871642 1103 52-53 0.34644087803100376 1103 54-55 0.5143328001959873 1103 56-57 0.41266275767345917 1103 58-59 0.4011725621400828 1103 60-61 0.5821490591982794 1103 62-63 0.4888817235453331 1103 64-65 0.5505876156023746 1103 66-67 0.4516167725636464 1103 68-69 0.4737010416900489 1103 70-71 0.4384915485723795 1103 72-73 0.4442275330558729 1103 74-75 0.3545692428601299 1103 76-77 0.3963884130722448 1103 78-79 0.44151451363778804 1103 80-81 0.22892644595545875 1103 82-83 0.37764821913086166 1103 84-85 0.24998411011991095 1103 86-87 0.08671985120383852 1103 88-89 0.18999006258944462 1103 90-91 0.21187013584295045 1103 92-93 0.3518367883500595 1103 94-95 0.3415227313102278 1103 96-97 0.3991437951196133 1103 98-99 0.13590061130775055 1103 100-101 -0.12000430560452102 1104 1 0.2528299888558436 1104 2 0.28327096537349306 1104 3 0.3682244359304079 1104 4 0.3083285898539998 1104 5 0.31588796545616304 1104 6 0.18359000953777382 1104 7 0.250453690874302 1104 8 0.3001795020662996 1104 9 0.2919453981456428 1104 10-11 0.30755275416371575 1104 12-13 0.34318655804993625 1104 14-15 0.4238205830592463 1104 16-17 0.41669810829212395 1104 18-19 0.38008474983529794 1104 20-21 0.44741133653876375 1104 22-23 0.42384840023805737 1104 24-25 0.52978337956214 1104 26-27 0.3966687447637369 1104 28-29 0.36860579308422103 1104 30-31 0.3134948997441782 1104 32-33 0.49058515057053853 1104 34-35 0.4957678427627741 1104 36-37 0.5493256685423304 1104 38-39 0.5802013181780268 1104 40-41 0.46003017879308317 1104 42-43 0.42661053283337935 1104 44-45 0.5370752931408731 1104 46-47 0.5997605002367123 1104 48-49 0.4881346200702197 1104 50-51 0.39560628999148406 1104 52-53 0.5355527514824985 1104 54-55 0.5562224928567971 1104 56-57 0.43636640613510735 1104 58-59 0.5408469102147606 1104 60-61 0.6048931531318757 1104 62-63 0.4520101364686582 1104 64-65 0.47773339662838055 1104 66-67 0.5283788146415205 1104 68-69 0.6247950001424982 1104 70-71 0.4370834561634709 1104 72-73 0.3483244497204723 1104 74-75 0.4212351637590288 1104 76-77 0.33000654682983566 1104 78-79 0.299286905315018 1104 80-81 0.3421851196489136 1104 82-83 0.42925476862912504 1104 84-85 0.398384189668473 1104 86-87 0.3064729192095683 1104 88-89 0.2600227935081456 1104 90-91 0.30140079932767705 1104 92-93 0.2574099937427903 1104 94-95 0.1303766519771088 1104 96-97 0.09699084643672506 1104 98-99 0.1076438020337136 1104 100-101 -0.2491466726714151 1105 1 0.24539593096241674 1105 2 0.31995869068312643 1105 3 0.29591405294864614 1105 4 0.290690727127803 1105 5 0.25106161002909744 1105 6 0.13335336749901217 1105 7 0.32334229422226457 1105 8 0.20194057570017065 1105 9 0.39668894670544574 1105 10-11 0.3523526970555295 1105 12-13 0.3552006923255533 1105 14-15 0.37189853573084264 1105 16-17 0.31329517435915477 1105 18-19 0.40510455923673305 1105 20-21 0.39876587128552643 1105 22-23 0.4045544001666741 1105 24-25 0.40827001267775387 1105 26-27 0.4514797523662608 1105 28-29 0.5673215727893961 1105 30-31 0.5130230433712555 1105 32-33 0.41989235690954274 1105 34-35 0.5427229057602432 1105 36-37 0.5227103992415465 1105 38-39 0.6881550605487305 1105 40-41 0.6752123895936677 1105 42-43 0.4758385015666491 1105 44-45 0.5753042255747545 1105 46-47 0.5739897182115143 1105 48-49 0.5851621390765374 1105 50-51 0.5033715753539454 1105 52-53 0.46214303595264283 1105 54-55 0.5620125488942094 1105 56-57 0.5051260877569845 1105 58-59 0.6737205226643468 1105 60-61 0.6758871924651402 1105 62-63 0.6611295996517228 1105 64-65 0.5611591738350796 1105 66-67 0.6096137791273719 1105 68-69 0.47685125170404774 1105 70-71 0.3793242385455926 1105 72-73 0.5678254669600022 1105 74-75 0.6020389614532888 1105 76-77 0.3363584046304595 1105 78-79 0.31966524703611654 1105 80-81 0.382555608994668 1105 82-83 0.3183485331292495 1105 84-85 0.36562764899673894 1105 86-87 0.1700864395722057 1105 88-89 0.28385332497118654 1105 90-91 0.19738181085638828 1105 92-93 0.25571959148701495 1105 94-95 0.2023772230589529 1105 96-97 0.16116045774414545 1105 98-99 0.3290957776199619 1105 100-101 -0.05715752322643297 1106 1 0.2341293548768988 1106 2 0.34220502768567584 1106 3 0.2777643649712971 1106 4 0.32707420337888493 1106 5 0.30063602183569316 1106 6 0.26565519110391733 1106 7 0.28602708314634384 1106 8 0.3018328881342782 1106 9 0.35837553444142145 1106 10-11 0.328728426433436 1106 12-13 0.4498191023024418 1106 14-15 0.49431051113075597 1106 16-17 0.4846563858857138 1106 18-19 0.4811367283536896 1106 20-21 0.5473473570122067 1106 22-23 0.5808305053583638 1106 24-25 0.5562906790444941 1106 26-27 0.5969804995476622 1106 28-29 0.5673463657979241 1106 30-31 0.5885149660502265 1106 32-33 0.5310357215393466 1106 34-35 0.6050037721138679 1106 36-37 0.5729096852933324 1106 38-39 0.7508323889626496 1106 40-41 0.8030189920698021 1106 42-43 0.6698440193298296 1106 44-45 0.657671272124567 1106 46-47 0.5356425452550369 1106 48-49 0.577064998712693 1106 50-51 0.6906860705223252 1106 52-53 0.6315076943856184 1106 54-55 0.6120560685368304 1106 56-57 0.6279691902139035 1106 58-59 0.6310568793882609 1106 60-61 0.6842002354715859 1106 62-63 0.6344080115728659 1106 64-65 0.7157484802864644 1106 66-67 0.6564429492699588 1106 68-69 0.6804750249588025 1106 70-71 0.5831665519970457 1106 72-73 0.5588756430355843 1106 74-75 0.5750256598911747 1106 76-77 0.6909852397087235 1106 78-79 0.7832199013017593 1106 80-81 0.7267024619781566 1106 82-83 0.5170711280001861 1106 84-85 0.673213480658994 1106 86-87 0.5488917330679257 1106 88-89 0.5786063255657687 1106 90-91 0.6149179283918293 1106 92-93 0.8630630696069979 1106 94-95 0.8661951052235857 1106 96-97 0.6751269095631898 1106 98-99 0.6202369116325812 1106 100-101 0.5816996019752416 1107 1 0.21708506331964372 1107 2 0.24451375378895435 1107 3 0.208937448408129 1107 4 0.28515875104849187 1107 5 0.2383313748824918 1107 6 0.18885863507205158 1107 7 0.2540356202247267 1107 8 0.18758026491298807 1107 9 0.24613626315244375 1107 10-11 0.32183102070003855 1107 12-13 0.31609400059684134 1107 14-15 0.4244780112309101 1107 16-17 0.47341978425478715 1107 18-19 0.3583900035105714 1107 20-21 0.3589230379935415 1107 22-23 0.33996324891457874 1107 24-25 0.3089783654743954 1107 26-27 0.4009307193482883 1107 28-29 0.39165372975644885 1107 30-31 0.40693633496929493 1107 32-33 0.3996051417537245 1107 34-35 0.459625776943291 1107 36-37 0.547880093244764 1107 38-39 0.6671784902785376 1107 40-41 0.6429895639156555 1107 42-43 0.6173409872830007 1107 44-45 0.8515969806037376 1107 46-47 0.8400192547649183 1107 48-49 0.8414587532425486 1107 50-51 0.8382402194185872 1107 52-53 0.8253907903148487 1107 54-55 0.8774936484353546 1107 56-57 0.9038603206119546 1107 58-59 0.9085290446521128 1107 60-61 0.7845025296293002 1107 62-63 0.9130915387265333 1107 64-65 0.9132893355239844 1107 66-67 0.7253051941864967 1107 68-69 0.8903545807684949 1107 70-71 0.8542559400152712 1107 72-73 0.8301453149189442 1107 74-75 0.9293796062867798 1107 76-77 0.9193830442518731 1107 78-79 0.9619296515630786 1107 80-81 0.9182961349250149 1107 82-83 0.9372006410152274 1107 84-85 0.8679765075340349 1107 86-87 0.9132607213294399 1107 88-89 0.9323789930749733 1107 90-91 0.9602358289406929 1107 92-93 1.0617486363357003 1107 94-95 1.1075307355069057 1107 96-97 1.0622575552707971 1107 98-99 1.025871237680505 1107 100-101 0.7819487214979794 1108 1 -0.04350987162909803 1108 2 -0.018631403990017503 1108 3 -0.03563084523057469 1108 4 -0.0362909587033684 1108 5 -0.05804589573158836 1108 6 -0.09333587402023369 1108 7 -0.03570814958806068 1108 8 -0.07142641648262327 1108 9 0.0018026681065350658 1108 10-11 -0.0020998787531709695 1108 12-13 -0.09778720623990012 1108 14-15 -0.08121795928499154 1108 16-17 0.08738252462420348 1108 18-19 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-1.0723033019881818 2316 86-87 -0.9481731004584759 2316 88-89 -1.0750130272545455 2316 90-91 -0.9999608346330753 2316 92-93 -1.052314564616129 2316 94-95 -1.0247446046419455 2316 96-97 -1.0097835050072312 2316 98-99 -0.8713719057044607 2316 100-101 -0.5130126451717523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8668.0 3 2663.0 4 2666.0 5 2882.0 6 3258.0 7 3947.0 8 4965.0 9 5864.0 10 7248.0 11 8929.0 12 11070.0 13 15024.0 14 13775.0 15 14365.0 16 17010.0 17 21327.0 18 18696.0 19 14822.0 20 15492.0 21 16336.0 22 18136.0 23 20112.0 24 22934.0 25 26284.0 26 30114.0 27 35280.0 28 41592.0 29 49108.0 30 58801.0 31 72020.0 32 89699.0 33 114577.0 34 155116.0 35 222152.0 36 347911.0 37 562750.0 38 560757.0 39 93220.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6544888824554 18.627090429407147 14.228825854587418 23.489594833550033 2 15.1527910372274 19.899676039503156 40.87227638271864 24.07525654055081 3 19.65381271847616 24.12163872250364 29.214091047475122 27.010457511545077 4 12.785091499983494 16.043987484548257 37.191762984047564 33.97915803142068 5 13.861567012381693 37.38909282729057 34.225494040625215 14.523846119702528 6 32.46329700331409 35.51218142502677 17.581112055620743 14.443409516038397 7 29.06317637121892 32.25726155073407 20.47835758738203 18.201204490664978 8 26.13729558155163 35.467762495339436 19.666294799567545 18.728647123541386 9 26.432338542281492 17.205952762394684 20.23512487617964 36.12658381914419 10-11 25.104023640461747 26.598470489105857 27.244983302879117 21.052522567553275 12-13 25.892660912856076 23.71691765122083 27.7801270824111 22.610294353511996 14-15 22.80726129240799 25.257990554139205 26.050644433377574 25.88410372007523 16-17 22.384165100108216 28.515481801038767 26.630662705546644 22.469690393306372 18-19 22.241949666178286 27.370117663836414 28.64280664296318 21.745126027022117 20-21 23.14502407407475 26.399707730870432 28.569582379969667 21.88568581508515 22-23 22.64848821498824 26.363906350542 28.428084571231626 22.559520863238134 24-25 22.533602011308016 26.46986512260556 28.64610538246368 22.350427483622738 26-27 22.375788457011357 26.700815719784888 28.592654195093655 22.3307416281101 28-29 22.239760675280554 26.98671104689715 28.61479892224678 22.158729355575513 30-31 22.38806982526086 26.624904626577994 28.554023429322108 22.433002118839042 32-33 22.272212838797735 26.679878414282328 28.487991192331197 22.55991755458874 34-35 22.755788315549495 26.753993284055653 28.30332800601673 22.186890394378114 36-37 22.727239300978642 26.469543565291314 28.42879403813967 22.374423095590377 38-39 22.42017763054875 26.799775627665433 28.434194284974918 22.3458524568109 40-41 22.633371507032976 26.786745983675964 28.292830743990216 22.28705176530084 42-43 22.413078477370966 26.67861529918789 28.13958100626945 22.768725217171696 44-45 22.54181182395126 26.82931268106171 28.04516866371104 22.583706831275993 46-47 22.640315173709542 26.9918044540531 27.7375476602801 22.63033271195726 48-49 22.62633817445123 26.890607209038812 27.897183194144986 22.585871422364967 50-51 22.55978991557243 27.197322026563164 27.68804359796056 22.554844459903844 52-53 22.711788687015392 27.217035201978184 27.492915021624924 22.578261089381495 54-55 22.50462774884605 26.99342464443999 27.656971218802905 22.84497638791105 56-57 22.6398727347042 27.203306163016023 27.530860658027446 22.62596044425233 58-59 22.734852604013824 26.9212598729043 27.66748725340364 22.676400269678233 60-61 22.643316383769857 27.011594324284015 27.90043619227726 22.44465309966887 62-63 22.455149474792467 26.999405938701404 27.940787234152054 22.604657352354078 64-65 22.378933805576313 26.976812683489342 27.76465855538363 22.87959495555072 66-67 22.48553066131837 27.05026272619661 27.524986476652135 22.939220135832883 68-69 22.43501053306736 26.98861295979193 27.751359295284832 22.82501721185588 70-71 22.481124350438417 26.89151395223066 27.652736962290625 22.97462473504029 72-73 22.509926061300263 26.789582209115153 27.769437751483967 22.931053978100614 74-75 22.278637832375587 27.1847550250956 27.501534345247354 23.03507279728146 76-77 22.425572162991656 26.87887948982152 27.56629314489036 23.129255202296463 78-79 22.4313100873617 26.840050815794946 27.61193700664678 23.11670209019657 80-81 22.430201296395545 26.99682701419536 27.513797268556196 23.059174420852898 82-83 22.473071474972528 26.894959727154184 27.614683587303418 23.01728521056987 84-85 22.450873427669343 26.794380282272705 27.524428859649525 23.230317430408427 86-87 22.30795616098716 26.80161673914714 27.636952798172675 23.253474301693025 88-89 22.369833290864584 26.67616422278332 27.573137249062103 23.380865237289985 90-91 22.289062023036223 26.675547310761 27.612777682563806 23.42261298363897 92-93 22.387903815555585 26.520210291995188 27.68299094043903 23.408894952010197 94-95 22.234194900325615 26.531413814737963 27.70747813474338 23.52691315019304 96-97 22.16620961037399 26.412612878009277 27.803384331290143 23.61779318032659 98-99 22.22031814023 26.4145531356553 27.537266888880392 23.82786183523431 100-101 22.298799736166966 25.91685288661136 27.954077021892697 23.830270355328977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1980.0 1 1106.0 2 271.0 3 321.0 4 312.5 5 336.0 6 525.0 7 1063.5 8 1689.0 9 1991.0 10 1976.5 11 1920.5 12 2071.0 13 2341.5 14 2569.0 15 2788.5 16 3026.5 17 3175.0 18 3306.0 19 3491.0 20 3836.0 21 4321.0 22 4967.5 23 5888.5 24 7187.0 25 9204.0 26 11941.0 27 15545.5 28 19029.5 29 22892.0 30 27561.0 31 32670.0 32 38419.0 33 43568.0 34 49159.0 35 54847.0 36 61059.0 37 67685.0 38 72658.0 39 76773.0 40 80696.5 41 86028.5 42 91363.5 43 96183.0 44 100608.5 45 104263.5 46 106966.5 47 111355.0 48 120698.0 49 121156.5 50 112844.0 51 106750.5 52 102303.0 53 98849.5 54 94064.5 55 85790.0 56 75366.0 57 66581.5 58 59118.0 59 50880.0 60 42374.0 61 34900.5 62 28068.0 63 21584.0 64 16173.0 65 12293.5 66 9429.0 67 7308.0 68 5451.0 69 3875.5 70 2910.5 71 2213.5 72 1693.5 73 1366.0 74 1075.0 75 795.5 76 577.5 77 367.5 78 233.0 79 159.5 80 99.0 81 67.5 82 57.5 83 49.0 84 34.0 85 22.5 86 19.0 87 16.5 88 12.5 89 10.0 90 7.5 91 2.0 92 6.5 93 7.5 94 3.0 95 2.5 96 2.5 97 2.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2840320015005741 2 0.11192025855594659 3 0.12280088598348049 4 0.12426629035082513 5 0.13119032598652855 6 0.05770029696419505 7 0.0670422498060171 8 0.07125528736213292 9 0.0688740052651979 10-11 0.06434956928102133 12-13 0.06830616107285184 14-15 0.06449610971775578 16-17 0.06499068369173461 18-19 0.0693685792391767 20-21 0.07323358325804818 22-23 0.08122003706007645 24-25 0.08944461907179822 26-27 0.0901223685916951 28-29 0.08358300160241969 30-31 0.08004771356620075 32-33 0.07323358325804818 34-35 0.2399599651526841 36-37 0.3639331746300403 38-39 0.3349914383749838 40-41 0.31967796273623234 42-43 0.3431427501683383 44-45 0.3563680245836237 46-47 0.36092909567698384 48-49 0.37021609585503046 50-51 0.36464755925912085 52-53 0.3648673699142226 54-55 0.3523198450188341 56-57 0.3299357933076448 58-59 0.31511689164287215 60-61 0.2721255910158989 62-63 0.3430511623953793 64-65 0.3706740347198257 66-67 0.3755465042412466 68-69 0.3870865636340856 70-71 0.38747123228051356 72-73 0.37146168956727343 74-75 0.3744474509657381 76-77 0.35863940135300787 78-79 0.33733608536273524 80-81 0.33543105968518716 82-83 0.34990192781271545 84-85 0.35662447034790895 86-87 0.3509277108698567 88-89 0.3654352141065686 90-91 0.38833215734632853 92-93 0.38285520852337795 94-95 0.38349632293409125 96-97 0.3880207589182678 98-99 0.37800105655654886 100-101 0.35609326126474655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2729622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.61338001596291 #Duplication Level Percentage of deduplicated Percentage of total 1 74.83153310839933 43.1129755415132 2 14.175483811047943 16.333950714320704 3 4.888550572716314 8.449377656194743 4 2.2003665024676526 5.070822059242562 5 1.164490380229414 3.354511340054519 6 0.6880635198975629 2.3784999028187612 7 0.4192283571278364 1.6907213856871697 8 0.3043605004127684 1.4028189737703571 9 0.21848914017112064 1.1329108075835785 >10 1.0195020471057075 10.402962301019922 >50 0.05993671911313073 2.3373484997622715 >100 0.028274927546167145 2.8901873072138793 >500 0.0010832234817683716 0.4624445107765869 >1k 5.097522266971206E-4 0.5631786329803133 >5k 1.2743805667428016E-4 0.41729036706128814 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6355 0.23281611886187903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5012 0.1836151672282829 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3704 0.1356964444161133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3319 0.12159192738042117 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.66351091836159E-5 2 0.0 0.0 0.0 0.0 3.66351091836159E-5 3 0.0 0.0 0.0 0.0 3.66351091836159E-5 4 0.0 0.0 0.0 0.0 3.66351091836159E-5 5 0.0 0.0 0.0 0.0 3.66351091836159E-5 6 0.0 0.0 0.0 0.0 3.66351091836159E-5 7 0.0 0.0 0.0 0.0 3.66351091836159E-5 8 0.0 0.0 0.0 0.0 3.66351091836159E-5 9 0.0 0.0 0.0 0.0 3.66351091836159E-5 10-11 0.0 0.0 0.0 0.0 3.66351091836159E-5 12-13 0.0 0.0 0.0 0.0 1.099053275508477E-4 14-15 0.0 0.0 0.0 0.0 1.099053275508477E-4 16-17 0.0 0.0 0.0 0.0 1.099053275508477E-4 18-19 0.0 0.0 0.0 0.0 1.099053275508477E-4 20-21 0.0 0.0 0.0 3.66351091836159E-5 1.465404367344636E-4 22-23 3.66351091836159E-5 0.0 0.0 1.2822288214265566E-4 1.465404367344636E-4 24-25 3.66351091836159E-5 0.0 0.0 3.297159826525431E-4 1.465404367344636E-4 26-27 3.66351091836159E-5 0.0 0.0 7.693372928559339E-4 1.465404367344636E-4 28-29 3.66351091836159E-5 0.0 0.0 0.0023080118785678014 1.465404367344636E-4 30-31 3.66351091836159E-5 0.0 0.0 0.00652104943468363 1.465404367344636E-4 32-33 3.66351091836159E-5 0.0 0.0 0.015936272494872915 1.831755459180795E-4 34-35 3.66351091836159E-5 0.0 0.0 0.03126806568821617 1.831755459180795E-4 36-37 3.66351091836159E-5 0.0 0.0 0.05724235809939984 1.831755459180795E-4 38-39 3.66351091836159E-5 0.0 0.0 0.09708303933658213 1.831755459180795E-4 40-41 3.66351091836159E-5 0.0 0.0 0.1412466634574311 1.831755459180795E-4 42-43 3.66351091836159E-5 0.0 0.0 0.18889062295072356 1.831755459180795E-4 44-45 3.66351091836159E-5 0.0 0.0 0.2484409929286912 1.831755459180795E-4 46-47 3.66351091836159E-5 0.0 0.0 0.3186888147882747 1.831755459180795E-4 48-49 3.66351091836159E-5 0.0 0.0 0.3989929741187608 1.831755459180795E-4 50-51 3.66351091836159E-5 0.0 0.0 0.4886940389548443 2.198106551016954E-4 52-53 3.66351091836159E-5 0.0 0.0 0.5761603621307272 2.198106551016954E-4 54-55 3.66351091836159E-5 0.0 0.0 0.6724191115106781 2.564457642853113E-4 56-57 3.66351091836159E-5 0.0 0.0 0.788790535832434 2.747633188771193E-4 58-59 3.66351091836159E-5 0.0 0.0 0.9204204831291659 2.930808734689272E-4 60-61 3.66351091836159E-5 0.0 0.0 1.0707892887733172 2.930808734689272E-4 62-63 3.66351091836159E-5 0.0 0.0 1.2266350432404194 3.66351091836159E-4 64-65 3.66351091836159E-5 0.0 0.0 1.3899360424263874 3.66351091836159E-4 66-67 3.66351091836159E-5 0.0 0.0 1.5813728054653722 3.66351091836159E-4 68-69 3.66351091836159E-5 0.0 0.0 1.8025939122706367 3.66351091836159E-4 70-71 3.66351091836159E-5 0.0 0.0 2.0422241614406684 4.029862010197749E-4 72-73 3.66351091836159E-5 0.0 0.0 2.292441957164765 4.213037556115829E-4 74-75 3.66351091836159E-5 0.0 0.0 2.555024102238332 4.396213102033908E-4 76-77 3.66351091836159E-5 0.0 0.0 2.8399353463593124 4.396213102033908E-4 78-79 3.66351091836159E-5 0.0 0.0 3.1659328654297187 4.396213102033908E-4 80-81 3.66351091836159E-5 0.0 0.0 3.530360614033738 4.396213102033908E-4 82-83 3.66351091836159E-5 0.0 0.0 3.908691386572939 4.762564193870067E-4 84-85 3.66351091836159E-5 0.0 0.0 4.292499107935091 5.128915285706226E-4 86-87 3.66351091836159E-5 0.0 0.0 4.704918849569648 5.128915285706226E-4 88-89 3.66351091836159E-5 0.0 0.0 5.16157548554342 5.128915285706226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10720 0.0 27.383614 1 TACATGG 10765 0.0 26.42925 2 GTATCAA 13795 0.0 25.067251 1 ACATGGG 11175 0.0 24.695799 3 GGTATCA 10030 0.0 23.347643 1 ATCAACG 15475 0.0 22.031591 3 TCAACGC 15575 0.0 21.829245 4 CAACGCA 15675 0.0 21.750486 5 TATCAAC 15815 0.0 21.587927 2 AACGCAG 16125 0.0 21.13148 6 GAGTACT 7260 0.0 20.726017 12-13 CATGGGA 6680 0.0 20.372911 4 CATGGGG 5155 0.0 19.408924 4 GTACTTT 7785 0.0 19.267431 14-15 ACGCAGA 17870 0.0 18.988785 7 CGCAGAG 17995 0.0 18.856884 8 GTGTAGC 1850 0.0 18.486633 1 AGAGTAC 13300 0.0 18.173027 10-11 AGTACTT 7780 0.0 18.061502 12-13 AGTACAT 7855 0.0 17.325403 2 >>END_MODULE