FastQCFastQC Report
Fri 27 May 2016
SRR939308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729622
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.2292625132710683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.1965839958792829No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39510.14474531638446642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.12818624703347203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131050.025.2671131
GGTATCA97000.023.493471
GTACATG104250.023.456891
TACATGG104500.023.0670322
ACATGGG107250.021.7234343
ATCAACG151850.021.342743
TATCAAC155000.021.1232972
TCAACGC154250.021.041434
CAACGCA154200.020.9867085
GAGTACT73000.020.6072712-13
AACGCAG158750.020.5346476
GTACTTT76000.019.6377214-15
AGTACTT76600.019.1741412-13
CATGGGG46450.019.0007844
GTATAGG8050.018.3210011
AGAGTAC136550.018.08877210-11
ACGCAGA178350.018.0651257
CGCAGAG178100.017.9039828
AGTACAT81100.017.6697482
GAGTACA92900.017.3094941
Produced by FastQC (version 0.11.5)