##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2729622 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30978098799028 33.0 31.0 34.0 31.0 34.0 2 32.511763533558856 34.0 31.0 34.0 31.0 34.0 3 32.604850415185695 34.0 31.0 34.0 31.0 34.0 4 36.12755575680442 37.0 35.0 37.0 35.0 37.0 5 36.06215805704966 37.0 35.0 37.0 35.0 37.0 6 36.09637891253807 37.0 35.0 37.0 35.0 37.0 7 36.0458169665983 37.0 35.0 37.0 35.0 37.0 8 36.01948438281931 37.0 35.0 37.0 35.0 37.0 9 37.73823225340359 39.0 38.0 39.0 35.0 39.0 10-11 37.657628052528885 39.0 37.0 39.0 35.0 39.0 12-13 37.584225398241955 39.0 37.0 39.0 35.0 39.0 14-15 38.89914226218868 40.0 38.0 41.0 35.0 41.0 16-17 38.845206589044196 40.0 38.0 41.0 35.0 41.0 18-19 38.94919461375971 40.0 38.0 41.0 35.0 41.0 20-21 38.94911328381732 40.0 38.0 41.0 35.0 41.0 22-23 38.895220290575026 40.0 38.0 41.0 35.0 41.0 24-25 38.837619824283365 40.0 38.0 41.0 35.0 41.0 26-27 38.678941626349726 40.0 38.0 41.0 34.0 41.0 28-29 38.54126432158006 40.0 38.0 41.0 34.0 41.0 30-31 38.37977236408558 40.0 38.0 41.0 34.0 41.0 32-33 38.20227214610668 40.0 38.0 41.0 33.5 41.0 34-35 38.180599181864736 40.0 38.0 41.0 34.0 41.0 36-37 38.11390404971824 40.0 38.0 41.0 33.0 41.0 38-39 38.02577444789058 40.0 38.0 41.0 33.0 41.0 40-41 37.87485519973095 40.0 37.5 41.0 33.0 41.0 42-43 37.72250040481796 40.0 37.0 41.0 33.0 41.0 44-45 37.52977371958461 40.0 37.0 41.0 32.5 41.0 46-47 37.28850954454499 40.0 37.0 41.0 32.0 41.0 48-49 37.015663340931454 40.0 36.5 41.0 31.0 41.0 50-51 36.80020860031169 40.0 36.0 41.0 31.0 41.0 52-53 36.58758318917418 39.0 36.0 41.0 30.5 41.0 54-55 36.32615999577963 39.0 35.0 41.0 30.0 41.0 56-57 36.09230362299249 39.0 35.0 40.0 29.5 41.0 58-59 35.80792413748131 38.5 35.0 40.0 29.0 41.0 60-61 35.48824104582979 38.0 35.0 40.0 28.0 41.0 62-63 35.1211195176475 38.0 34.0 40.0 28.0 41.0 64-65 34.710106930556684 37.0 34.0 40.0 27.5 41.0 66-67 34.33792023217867 36.5 34.0 39.0 26.5 41.0 68-69 33.94854104341187 36.0 33.0 39.0 26.0 40.0 70-71 33.555209842241894 36.0 33.0 39.0 26.0 40.0 72-73 33.06011015444629 35.0 32.5 38.0 25.5 40.0 74-75 32.5578662540088 35.0 32.0 37.0 24.5 39.0 76-77 30.53827764430386 33.5 29.5 35.5 20.5 37.5 78-79 31.525432092795263 34.5 31.0 36.0 22.5 38.5 80-81 31.566369812376955 35.0 31.0 36.0 23.0 37.5 82-83 31.26095041731053 34.5 31.0 35.5 22.0 37.0 84-85 30.888118208308697 34.0 31.0 35.0 20.5 36.5 86-87 30.359063086390716 34.0 30.5 35.0 18.5 36.0 88-89 29.868492230792395 34.0 30.0 35.0 13.0 36.0 90-91 29.461484960188628 34.0 29.5 35.0 7.5 35.0 92-93 29.120209501535378 34.0 29.0 35.0 3.5 35.0 94-95 28.743072117677833 34.0 29.0 35.0 2.0 35.0 96-97 28.2088743423082 34.0 29.0 35.0 2.0 35.0 98-99 27.484990046240835 33.0 28.0 35.0 2.0 35.0 100-101 24.170278888432172 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09540628584914401 1101 2 0.13969660139392914 1101 3 0.15566160916015548 1101 4 0.1680003817175333 1101 5 0.19007516265647695 1101 6 0.11071426375252713 1101 7 0.1688620858304617 1101 8 0.24119880584049724 1101 9 0.20728451373609147 1101 10-11 0.20112693807014637 1101 12-13 0.2729670834172637 1101 14-15 0.2540220034501104 1101 16-17 0.26571838368204936 1101 18-19 0.12680029573527918 1101 20-21 0.16192489232148688 1101 22-23 0.2101061946831777 1101 24-25 0.29367636774537687 1101 26-27 0.3625444880042821 1101 28-29 0.30353308796082246 1101 30-31 0.23369858018489253 1101 32-33 0.2999126024962635 1101 34-35 0.30533022815571087 1101 36-37 0.3708363546138429 1101 38-39 0.33578024634991976 1101 40-41 0.3905823096519825 1101 42-43 0.42108722388472586 1101 44-45 0.45919896149775496 1101 46-47 0.3413184960754876 1101 48-49 0.38111546038648925 1101 50-51 0.39384917154600174 1101 52-53 0.27720476152512674 1101 54-55 0.23713488578501796 1101 56-57 0.2640693604092874 1101 58-59 0.23725078257858456 1101 60-61 0.29681663776663925 1101 62-63 0.297014132093949 1101 64-65 0.1693216353107445 1101 66-67 0.2502554045474028 1101 68-69 0.3024268843245608 1101 70-71 0.29485664029255787 1101 72-73 0.18898127054660563 1101 74-75 0.23137799602418596 1101 76-77 0.2594806995712773 1101 78-79 0.48127166253718556 1101 80-81 0.5548159930576411 1101 82-83 0.49663538582936084 1101 84-85 0.2976282136358144 1101 86-87 0.4598172618060836 1101 88-89 0.6835357638686617 1101 90-91 0.6332365528755126 1101 92-93 0.46215881943262715 1101 94-95 0.5887306704612456 1101 96-97 0.4023989864994917 1101 98-99 0.5078171176919106 1101 100-101 0.49678392184811315 1102 1 0.1516508348367509 1102 2 0.18272791402422683 1102 3 0.17366802434108308 1102 4 0.136186974180319 1102 5 0.14742866896330042 1102 6 0.16861722857296968 1102 7 0.15890628786234373 1102 8 0.1762731163747091 1102 9 0.2884408301226671 1102 10-11 0.31126708499072464 1102 12-13 0.23632311111008875 1102 14-15 0.2534538784781688 1102 16-17 0.3444086417352352 1102 18-19 0.233082973085196 1102 20-21 0.23805275473272047 1102 22-23 0.1796898548857584 1102 24-25 0.23325113309774537 1102 26-27 0.3137922417540935 1102 28-29 0.23221705315494745 1102 30-31 0.2354038389292299 1102 32-33 0.20746157631832318 1102 34-35 0.33831258729200897 1102 36-37 0.32981787305244836 1102 38-39 0.18997788384093184 1102 40-41 0.19758385776601273 1102 42-43 0.1393703304217695 1102 44-45 0.2311272773483708 1102 46-47 0.26939553622569434 1102 48-49 0.12802868928429234 1102 50-51 0.22552535586738287 1102 52-53 0.25949495919514476 1102 54-55 0.1828638374972158 1102 56-57 0.26635220109071867 1102 58-59 0.2936620216248542 1102 60-61 0.1944255457682189 1102 62-63 0.07236312946119483 1102 64-65 0.18711946696121373 1102 66-67 0.11876187222774348 1102 68-69 0.20256380703870036 1102 70-71 0.13000335405903485 1102 72-73 0.22889814824205956 1102 74-75 0.19476601830939444 1102 76-77 0.26557681839742386 1102 78-79 0.24767454763629715 1102 80-81 0.2302066364247004 1102 82-83 0.34289535939228344 1102 84-85 0.29898842572637463 1102 86-87 0.4229969657093875 1102 88-89 0.40913096050813635 1102 90-91 0.5919683897575325 1102 92-93 0.4798274353536307 1102 94-95 0.4919642631570049 1102 96-97 0.4768463373649183 1102 98-99 0.48711873760817426 1102 100-101 0.2893130431139781 1103 1 0.15650820179717329 1103 2 0.1590726857030731 1103 3 0.14190588204814958 1103 4 0.14099296148452112 1103 5 0.15296746510162507 1103 6 0.12777818667727558 1103 7 0.1689569789965688 1103 8 0.10211808682535661 1103 9 0.12566229443248744 1103 10-11 0.17074960708484355 1103 12-13 0.19258449204992445 1103 14-15 0.22440880312307598 1103 16-17 0.14197270974459997 1103 18-19 0.16733067977067861 1103 20-21 0.22778607874736423 1103 22-23 0.23491044314648946 1103 24-25 0.22384995347619707 1103 26-27 0.2855759877220052 1103 28-29 0.2345201419199654 1103 30-31 0.2982666753146077 1103 32-33 0.30625583245334553 1103 34-35 0.30750520702822826 1103 36-37 0.374179275824325 1103 38-39 0.35172951838868727 1103 40-41 0.3250549683865884 1103 42-43 0.3912113482717814 1103 44-45 0.4547530791391452 1103 46-47 0.37041408168666123 1103 48-49 0.33156867839941384 1103 50-51 0.36973049182625317 1103 52-53 0.28014369914799886 1103 54-55 0.2873450603953174 1103 56-57 0.35533389436350404 1103 58-59 0.19404448002203623 1103 60-61 0.2037486179325967 1103 62-63 0.24440738931562578 1103 64-65 0.39451085434686917 1103 66-67 0.4290932658204696 1103 68-69 0.4526953700977998 1103 70-71 0.4824992808805675 1103 72-73 0.363155957863313 1103 74-75 0.3660092226146219 1103 76-77 0.5871371753078485 1103 78-79 0.5379890672503542 1103 80-81 0.6441716615834316 1103 82-83 0.5669639431025466 1103 84-85 0.4794656960238477 1103 86-87 0.4643195299590417 1103 88-89 0.6733612223298664 1103 90-91 0.7019550206671887 1103 92-93 0.5429381742553652 1103 94-95 0.37716446563090855 1103 96-97 0.15046399106880415 1103 98-99 -0.09460441150458365 1103 100-101 -0.06656036087301231 1104 1 0.15087649506620693 1104 2 0.114446108197086 1104 3 0.09813445158270895 1104 4 0.0956295654238346 1104 5 0.09752891222255045 1104 6 0.07737754365972904 1104 7 0.18092572304078658 1104 8 0.1781295550763602 1104 9 0.1484690328585856 1104 10-11 0.13224710876281875 1104 12-13 0.21222834650919964 1104 14-15 0.26079332098421304 1104 16-17 0.18801765468207776 1104 18-19 0.16482954040325382 1104 20-21 0.26238481425667715 1104 22-23 0.22842068916699532 1104 24-25 0.19741704357323187 1104 26-27 0.20016469679799798 1104 28-29 0.3107664441547797 1104 30-31 0.24213381264360123 1104 32-33 0.26525733227518344 1104 34-35 0.19575704451714415 1104 36-37 0.3187334309937313 1104 38-39 0.4267181247144478 1104 40-41 0.5188204327098802 1104 42-43 0.481642567767814 1104 44-45 0.5382146845285618 1104 46-47 0.6469107133022405 1104 48-49 0.640243200486573 1104 50-51 0.5180114142581189 1104 52-53 0.5328978195149148 1104 54-55 0.5927503023140943 1104 56-57 0.7042384443755267 1104 58-59 0.7580900215737287 1104 60-61 0.6252736301549007 1104 62-63 0.5902836250113808 1104 64-65 0.599714076063627 1104 66-67 0.5873229788656076 1104 68-69 0.6918950784197406 1104 70-71 0.7116559832612168 1104 72-73 0.6822817523131377 1104 74-75 0.6271981421628539 1104 76-77 0.6987157999463598 1104 78-79 0.8286233013054165 1104 80-81 0.9015026416051519 1104 82-83 0.9258897060643996 1104 84-85 0.8464624063593611 1104 86-87 0.8501817617933796 1104 88-89 1.1179903685914994 1104 90-91 1.1440343040672225 1104 92-93 1.1432789901252995 1104 94-95 1.061849875927475 1104 96-97 0.863643281926521 1104 98-99 0.96701581040276 1104 100-101 0.5946505173928287 1105 1 0.12515925196380806 1105 2 0.06917216737544152 1105 3 0.1264087136378862 1105 4 0.08081713011669933 1105 5 0.08379296618978316 1105 6 0.13219426208068796 1105 7 0.1722238634555353 1105 8 0.1557688455071684 1105 9 0.10842620172034856 1105 10-11 0.17361984674333542 1105 12-13 0.05279014827241468 1105 14-15 0.12996418153316114 1105 16-17 0.20024237538736855 1105 18-19 0.1888106947024255 1105 20-21 0.18353554417066675 1105 22-23 0.20963138267450887 1105 24-25 0.16844891707859944 1105 26-27 0.10017683228716834 1105 28-29 0.15588333746955385 1105 30-31 0.26179830206073973 1105 32-33 0.2807900445570155 1105 34-35 0.2463748836862223 1105 36-37 0.2891199820163237 1105 38-39 0.15094270264928156 1105 40-41 0.26053367823938345 1105 42-43 0.2931477253507069 1105 44-45 0.32233289847010127 1105 46-47 0.30028259465998275 1105 48-49 0.30199142477896856 1105 50-51 0.3123655563861192 1105 52-53 0.27327544738371046 1105 54-55 0.3609260527513314 1105 56-57 0.3592943033183076 1105 58-59 0.31894378892926056 1105 60-61 0.3283139271174562 1105 62-63 0.38808210450597613 1105 64-65 0.2772791258548253 1105 66-67 0.4443833350779087 1105 68-69 0.3743371671515803 1105 70-71 0.37320004579468247 1105 72-73 0.3694024290451239 1105 74-75 0.43264554909436015 1105 76-77 0.40586003380437674 1105 78-79 0.4630609641529766 1105 80-81 0.5496191423011574 1105 82-83 0.5172042650821425 1105 84-85 0.44854471353000847 1105 86-87 0.5700240004342092 1105 88-89 0.6415731933050637 1105 90-91 0.6082617374112651 1105 92-93 0.6122135655616425 1105 94-95 0.41450576342435497 1105 96-97 0.44460912409377684 1105 98-99 0.5828390605723008 1105 100-101 0.6864205141804938 1106 1 0.09510108259213723 1106 2 0.07428480223923373 1106 3 0.13619102499436764 1106 4 0.07290326208332942 1106 5 0.11494142506692384 1106 6 0.058121644941259376 1106 7 0.09916376565502816 1106 8 0.05951228835928646 1106 9 0.15256062052022656 1106 10-11 0.20443695346706647 1106 12-13 0.18332782497912348 1106 14-15 0.22379803220389505 1106 16-17 0.2064902888936686 1106 18-19 0.13895195456405673 1106 20-21 0.24457834263063205 1106 22-23 0.1557915183804326 1106 24-25 0.1709954378809826 1106 26-27 0.252654663644968 1106 28-29 0.1977820900571814 1106 30-31 0.21687314454223383 1106 32-33 0.25505279166758044 1106 34-35 0.2262756664369192 1106 36-37 0.33990367469756677 1106 38-39 0.2775391953051951 1106 40-41 0.3448050388480439 1106 42-43 0.29259625046813653 1106 44-45 0.40313994480890614 1106 46-47 0.49580154096465634 1106 48-49 0.4001571189417561 1106 50-51 0.405671896237223 1106 52-53 0.38325203343918446 1106 54-55 0.3882593273048869 1106 56-57 0.47047082577105215 1106 58-59 0.49588214627564753 1106 60-61 0.3560993538021151 1106 62-63 0.4183016168297655 1106 64-65 0.3971525684733237 1106 66-67 0.291813566120112 1106 68-69 0.42423886174240977 1106 70-71 0.55253812521822 1106 72-73 0.605301198211663 1106 74-75 0.5273404481400945 1106 76-77 0.5376661479562124 1106 78-79 0.5922650935076668 1106 80-81 0.6282026551765547 1106 82-83 0.5582695505747672 1106 84-85 0.5018178804864313 1106 86-87 0.41378788056265137 1106 88-89 0.7475516241412379 1106 90-91 0.5967925971710066 1106 92-93 0.648482270530117 1106 94-95 0.6506208873856245 1106 96-97 0.6668081358399363 1106 98-99 0.8026376720131054 1106 100-101 0.8288900334749556 1107 1 0.09483448099762626 1107 2 0.0662801986269983 1107 3 0.13360950572948838 1107 4 0.06027027996967149 1107 5 0.019868819837597584 1107 6 0.09151895780114927 1107 7 0.07195364936283255 1107 8 0.09910553882274087 1107 9 0.05684846283194389 1107 10-11 0.12668216137623745 1107 12-13 0.08862380009630755 1107 14-15 0.1665307713895956 1107 16-17 0.09743056940808259 1107 18-19 0.15930982714160535 1107 20-21 0.1734292242005182 1107 22-23 0.18002822563221343 1107 24-25 0.13749141154133326 1107 26-27 0.13462044977367071 1107 28-29 0.16281175494771816 1107 30-31 0.25820841061572253 1107 32-33 0.2688093611023561 1107 34-35 0.35377824322145557 1107 36-37 0.328305048497036 1107 38-39 0.22579044181333785 1107 40-41 0.22604677583755262 1107 42-43 0.20180165388515547 1107 44-45 0.1489455223182219 1107 46-47 0.2468164116519631 1107 48-49 0.35862627219032817 1107 50-51 0.3044714606422687 1107 52-53 0.35547156455545803 1107 54-55 0.37832368318159837 1107 56-57 0.33554965898844813 1107 58-59 0.3885724550163161 1107 60-61 0.4034466315157559 1107 62-63 0.4179814763904517 1107 64-65 0.5130037086321977 1107 66-67 0.4480927256695111 1107 68-69 0.43748225952874265 1107 70-71 0.3965623524560158 1107 72-73 0.33005435500034963 1107 74-75 0.3629135505158274 1107 76-77 0.4637559548157384 1107 78-79 0.7831143215649412 1107 80-81 0.6790929661024911 1107 82-83 0.7234255962758134 1107 84-85 0.5524532445323622 1107 86-87 0.5333259168160964 1107 88-89 0.6520324976620913 1107 90-91 0.7929166761754054 1107 92-93 0.7014711584882498 1107 94-95 0.7585941689526017 1107 96-97 0.6525912051818459 1107 98-99 0.8378585834846213 1107 100-101 0.8022728507836661 1108 1 -0.08479210806353876 1108 2 -0.04581064138609037 1108 3 -0.07873401098523658 1108 4 -0.07155673129614115 1108 5 -0.11149680011922669 1108 6 -0.03483189342016857 1108 7 -0.10631081704549672 1108 8 -0.09135328217849548 1108 9 -0.0483837286573916 1108 10-11 -0.011813183276743189 1108 12-13 -0.09225662160270787 1108 14-15 -0.0892012957979702 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9 1204.0 10 2072.0 11 2977.0 12 4582.0 13 6382.0 14 7971.0 15 10337.0 16 13182.0 17 18280.0 18 17847.0 19 12643.0 20 12295.0 21 13190.0 22 14180.0 23 15681.0 24 18228.0 25 21917.0 26 26397.0 27 33029.0 28 40082.0 29 49240.0 30 60461.0 31 73984.0 32 92719.0 33 119376.0 34 161409.0 35 231303.0 36 369096.0 37 636580.0 38 587032.0 39 54816.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66464860575625 19.302115841349554 14.710782359845487 23.322453193048712 2 15.537352790972522 20.42055639938424 39.90354708454137 24.13854372510186 3 19.662649260593593 24.15048677069572 29.4807486164751 26.70611535223558 4 13.158561881461974 16.618418227871846 36.7911747487381 33.43184514192807 5 13.839901642058864 37.54182813591039 34.138646303407576 14.479623918623163 6 32.51292669827544 35.895519599417064 17.23890707211475 14.352646630192753 7 29.056330876582916 32.31861407916554 20.31339870502216 18.311656339229387 8 26.109695774726315 35.74377697717853 19.49584960848059 18.650677639614567 9 26.218208968128188 17.110501014426173 20.134399561551014 36.53689045589463 10-11 25.112524737857477 26.727785019317697 27.070140114638587 21.089550128186247 12-13 25.78336853967326 23.804120130919227 27.734426231910497 22.678085097497018 14-15 22.644095726889017 25.389687777174096 25.984363030337892 25.981853465599 16-17 22.31591299257109 28.710773642789473 26.55711117282128 22.41620219181816 18-19 22.199154315139605 27.54152406450417 28.547872196223505 21.71144942413272 20-21 23.066398937288753 26.46232701817321 28.606689864017802 21.864584180520232 22-23 22.592487164889498 26.49564664997571 28.411278191632395 22.500587993502396 24-25 22.5017708518608 26.59648225567053 28.618981004122006 22.282765888346667 26-27 22.37712344239371 26.78231045040114 28.586739615377994 22.25382649182716 28-29 22.204539676189597 27.12412194802064 28.590533780867826 22.080804594921936 30-31 22.387110742806147 26.70109634227743 28.515340219268452 22.39645269564797 32-33 22.262368196035933 26.799388340217075 28.467110098028225 22.47113336571877 34-35 22.691496550601045 26.788735144668497 28.362685418588928 22.15708288614153 36-37 22.68038649445978 26.551871846651004 28.487293897531114 22.2804477613581 38-39 22.447939154983658 26.86762219096209 28.443740837131905 22.240697816922346 40-41 22.654089997627448 26.773373541543954 28.32075452685886 22.25178193396974 42-43 22.386452044157217 26.795377645037533 28.0918359926059 22.726334318199346 44-45 22.528998227400177 26.904464068355637 28.034920051663125 22.53161765258106 46-47 22.652555285228658 27.075978058460215 27.692584710602258 22.57888194570887 48-49 22.652861824824097 26.944078703937762 27.833286074042483 22.569773397195654 50-51 22.59060045676654 27.225985869105685 27.696142542813618 22.487271131314152 52-53 22.7791289012048 27.23055192817026 27.433254024413273 22.557065146211663 54-55 22.54304808504621 27.0806177558651 27.581199887750024 22.795134271338668 56-57 22.630408648232 27.284460501208684 27.419753984206235 22.665376866353082 58-59 22.728357632632264 27.006869674024326 27.61481466827349 22.64995802506992 60-61 22.68902329340398 27.092847191005475 27.736354907053162 22.481774608537386 62-63 22.468987496082278 27.16561806384818 27.75054225285936 22.614852187210175 64-65 22.3837993655897 27.067800425809953 27.69742984512688 22.850970363473472 66-67 22.46543660624072 27.08543527272274 27.48252322116396 22.966604899872582 68-69 22.392020687215766 27.04160688245219 27.687596957585676 22.878775472746366 70-71 22.52521503901091 26.985358040548483 27.5297925975804 22.959634322860207 72-73 22.50405001132025 26.867236562425127 27.6396145693433 22.989098856911323 74-75 22.303069802338932 27.29766246022343 27.261924911214813 23.137342826222827 76-77 22.368285770728637 26.986022403224254 27.4386070432165 23.20708478283061 78-79 22.407809579494888 26.905007359993434 27.55134227376538 23.135840786746297 80-81 22.393511275384476 27.115894235649847 27.363220168255637 23.12737432071004 82-83 22.452467777589717 26.99157612299432 27.414235377645696 23.141720721770266 84-85 22.318419913086867 26.999837340115224 27.41306305415182 23.26867969264609 86-87 22.215103587443537 26.910621506324716 27.4949139147408 23.379360991490948 88-89 22.250337831565297 26.796316289391548 27.447383112852012 23.505962766191143 90-91 22.2081345883154 26.78519397642432 27.48312930775058 23.523542127509693 92-93 22.227454936983946 26.74227420499981 27.459882723688484 23.570388134327757 94-95 22.080753686912267 26.73209454131278 27.448201151698136 23.738950620076814 96-97 21.87793767781766 26.65751887990352 27.622579243572922 23.841964198705902 98-99 21.93840392552522 26.65156567466118 27.346680236311112 24.06335016350249 100-101 21.96836478098018 26.212753002620147 27.82479293790775 23.99408927849192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 361.0 1 249.5 2 171.5 3 237.5 4 233.0 5 227.5 6 404.5 7 895.0 8 1416.0 9 1701.0 10 1691.0 11 1595.5 12 1705.0 13 1855.5 14 2068.0 15 2220.5 16 2373.5 17 2648.5 18 2848.0 19 3080.5 20 3463.5 21 4030.0 22 4775.0 23 5789.5 24 7139.0 25 9140.5 26 11960.0 27 15576.5 28 19094.5 29 22965.0 30 27695.5 31 32923.5 32 38630.0 33 43599.5 34 49004.0 35 54819.0 36 61247.0 37 67867.5 38 72736.0 39 76797.0 40 81030.5 41 86698.0 42 91724.5 43 96640.5 44 101415.0 45 105042.0 46 107435.5 47 112390.0 48 122318.5 49 121960.5 50 113616.5 51 107697.5 52 102773.0 53 99272.0 54 94931.5 55 86596.0 56 75975.0 57 66749.0 58 58793.5 59 50545.0 60 41877.0 61 34447.5 62 27837.5 63 21271.0 64 15816.0 65 11917.0 66 9018.5 67 7121.5 68 5284.0 69 3626.0 70 2701.0 71 2053.5 72 1586.5 73 1287.0 74 999.0 75 807.0 76 558.0 77 293.0 78 182.5 79 118.5 80 62.0 81 35.5 82 23.0 83 13.5 84 10.5 85 5.0 86 4.0 87 4.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27611881791691306 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023812820969350335 16-17 5.861617469378544E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2822288214265566E-4 26-27 0.0012089586030593247 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003242207162750007 36-37 0.021211728217313607 38-39 0.007162163845396908 40-41 0.01811606149129806 42-43 0.0054036786045833455 44-45 5.678441923460464E-4 46-47 1.831755459180795E-4 48-49 0.0 50-51 0.0 52-53 1.831755459180795E-5 54-55 0.0 56-57 1.831755459180795E-5 58-59 6.044793015296623E-4 60-61 0.001868390568364411 62-63 0.0013738165943855962 64-65 1.831755459180795E-5 66-67 0.0 68-69 4.945739739788147E-4 70-71 3.66351091836159E-5 72-73 0.0 74-75 0.0 76-77 0.22948232392617 78-79 0.0 80-81 9.341952841822055E-4 82-83 0.0 84-85 0.0 86-87 0.10329269034320503 88-89 0.35541551174484964 90-91 0.3569175512213779 92-93 0.0 94-95 1.831755459180795E-5 96-97 0.0 98-99 0.0 100-101 2.198106551016954E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2729622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.94743165442966 #Duplication Level Percentage of deduplicated Percentage of total 1 73.2936940036996 41.00593935972665 2 14.944245000188907 16.721842515502424 3 5.281621582320265 8.86479487504271 4 2.3291632717865394 5.212428118411408 5 1.1989329120688217 3.3538608578108366 6 0.7215997949139228 2.422299312467829 7 0.48210088918104343 1.8880614583597337 8 0.3222592455739285 1.4423661693404342 9 0.22588460130419985 1.1373896963929333 >10 1.1038851856978766 10.984177933624977 >50 0.0646436894646344 2.4555861973476016 >100 0.02967559308630886 2.8892330765479604 >500 0.001638736224285132 0.6245818339818011 >1k 5.243955917038366E-4 0.5711489332092059 >5k 1.3109889792595916E-4 0.4262896622334538 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 0.2292625132710683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5366 0.1965839958792829 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3951 0.14474531638446642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3499 0.12818624703347203 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 7.32702183672318E-5 20-21 0.0 0.0 0.0 5.495266377542385E-5 1.2822288214265566E-4 22-23 0.0 0.0 0.0 1.6485799132627156E-4 1.6485799132627156E-4 24-25 0.0 0.0 0.0 3.66351091836159E-4 2.198106551016954E-4 26-27 0.0 0.0 0.0 8.059724020395498E-4 2.198106551016954E-4 28-29 0.0 0.0 0.0 0.0023446469877514177 2.198106551016954E-4 30-31 0.0 0.0 0.0 0.006594319653050862 2.198106551016954E-4 32-33 0.0 0.0 0.0 0.016192718259158228 2.198106551016954E-4 34-35 0.0 0.0 0.0 0.03159778167086871 2.564457642853113E-4 36-37 0.0 0.0 0.0 0.05779188473715408 2.564457642853113E-4 38-39 0.0 0.0 0.0 0.09858507881311038 2.564457642853113E-4 40-41 0.0 0.0 0.0 0.14322495935334634 2.564457642853113E-4 42-43 0.0 0.0 0.0 0.19140012792980127 2.564457642853113E-4 44-45 0.0 0.0 0.0 0.2514450718817477 2.564457642853113E-4 46-47 0.0 0.0 0.0 0.32218746771531004 2.564457642853113E-4 48-49 0.0 0.0 0.0 0.40289461324681586 2.564457642853113E-4 50-51 0.0 0.0 0.0 0.492632313192083 2.564457642853113E-4 52-53 0.0 0.0 0.0 0.5798971432674561 2.564457642853113E-4 54-55 0.0 0.0 0.0 0.6765222437392431 2.564457642853113E-4 56-57 0.0 0.0 0.0 0.7936996404630385 2.564457642853113E-4 58-59 0.0 0.0 0.0 0.9265202288082379 2.564457642853113E-4 60-61 0.0 0.0 0.0 1.0780430403916732 2.564457642853113E-4 62-63 0.0 0.0 0.0 1.234896260361325 2.930808734689272E-4 64-65 0.0 0.0 0.0 1.3994428532595355 2.930808734689272E-4 66-67 0.0 0.0 0.0 1.5919237169102534 2.930808734689272E-4 68-69 0.0 0.0 0.0 1.813621080134905 2.930808734689272E-4 70-71 0.0 0.0 0.0 2.055119719873301 3.1139842806073514E-4 72-73 0.0 0.0 0.0 2.307315811493313 3.297159826525431E-4 74-75 0.0 0.0 0.0 2.5717663471352443 3.297159826525431E-4 76-77 0.0 0.0 0.0 2.858729157370508 3.297159826525431E-4 78-79 0.0 0.0 0.0 3.1875109447388685 3.297159826525431E-4 80-81 0.0 0.0 0.0 3.5543749281035986 3.297159826525431E-4 82-83 0.0 0.0 0.0 3.9342260576739196 3.297159826525431E-4 84-85 0.0 0.0 0.0 4.320506648905965 3.297159826525431E-4 86-87 0.0 0.0 0.0 4.734959639100213 3.480335372443511E-4 88-89 0.0 0.0 0.0 5.19460203647245 3.66351091836159E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13105 0.0 25.267113 1 GGTATCA 9700 0.0 23.49347 1 GTACATG 10425 0.0 23.45689 1 TACATGG 10450 0.0 23.067032 2 ACATGGG 10725 0.0 21.723434 3 ATCAACG 15185 0.0 21.34274 3 TATCAAC 15500 0.0 21.123297 2 TCAACGC 15425 0.0 21.04143 4 CAACGCA 15420 0.0 20.986708 5 GAGTACT 7300 0.0 20.60727 12-13 AACGCAG 15875 0.0 20.534647 6 GTACTTT 7600 0.0 19.63772 14-15 AGTACTT 7660 0.0 19.17414 12-13 CATGGGG 4645 0.0 19.000784 4 GTATAGG 805 0.0 18.321001 1 AGAGTAC 13655 0.0 18.088772 10-11 ACGCAGA 17835 0.0 18.065125 7 CGCAGAG 17810 0.0 17.903982 8 AGTACAT 8110 0.0 17.669748 2 GAGTACA 9290 0.0 17.309494 1 >>END_MODULE