FastQCFastQC Report
Fri 27 May 2016
SRR939307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173181
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.23062966223246018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37720.17357044811269748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.1152688156209722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56850.030.6364481
GTATCAA83550.029.8205551
ATCAACG99750.024.5730863
TCAACGC101000.024.3159074
TATCAAC102100.024.2396772
CAACGCA101350.024.137825
AACGCAG105450.023.3223676
GTACATG77450.022.9780251
TACATGG77150.022.6148642
GTATAGG5500.021.5714931
ACATGGG79150.021.2048113
ACGCAGA117950.020.6498577
TATCACG1150.003764863620.6136192
CGCAGAG118450.020.5231558
GAGTACT52950.020.49209212-13
TAGACCG1404.9159536E-420.318675
GTGCGAA1200.004789698419.7738671
CTAGACG1200.004816617419.7547194
AGAGTAC97300.019.47798210-11
GTACTTT58200.018.5662314-15
Produced by FastQC (version 0.11.5)