##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939307_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173181 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78157594788469 33.0 31.0 34.0 30.0 34.0 2 31.85433058728196 33.0 31.0 34.0 30.0 34.0 3 31.904893333781217 34.0 31.0 34.0 30.0 34.0 4 35.38667740975096 37.0 35.0 37.0 33.0 37.0 5 35.41772268393659 37.0 35.0 37.0 33.0 37.0 6 35.49842373920994 37.0 35.0 37.0 33.0 37.0 7 35.42554991967995 37.0 35.0 37.0 33.0 37.0 8 35.409110423844126 37.0 35.0 37.0 33.0 37.0 9 37.01795892748924 39.0 37.0 39.0 33.0 39.0 10-11 36.91006248444101 39.0 37.0 39.0 33.0 39.0 12-13 36.802683485636955 39.0 37.0 39.0 33.0 39.0 14-15 38.020970641653875 40.0 38.0 41.0 33.0 41.0 16-17 37.93649838646665 40.0 38.0 41.0 33.0 41.0 18-19 37.84199958494023 40.0 38.0 41.0 32.5 41.0 20-21 37.80507191991831 40.0 38.0 41.0 32.0 41.0 22-23 37.81585473092209 40.0 38.0 41.0 32.0 41.0 24-25 37.732460388711296 40.0 38.0 41.0 32.0 41.0 26-27 37.647604364293635 40.0 38.0 41.0 32.0 41.0 28-29 37.50131742362923 40.0 37.5 41.0 32.0 41.0 30-31 37.32090401121674 40.0 37.0 41.0 31.0 41.0 32-33 37.10914116219496 40.0 37.0 41.0 30.5 41.0 34-35 36.82755509090131 40.0 36.0 41.0 30.0 41.0 36-37 36.59137089823628 40.0 36.0 41.0 30.0 41.0 38-39 36.00196923311956 38.5 35.5 40.5 28.5 41.0 40-41 35.98360813020176 39.0 35.0 40.0 29.0 41.0 42-43 36.184104545364605 39.0 35.0 41.0 29.5 41.0 44-45 36.1095686461459 39.0 35.0 41.0 28.5 41.0 46-47 35.86086961923558 39.0 35.0 41.0 27.5 41.0 48-49 35.635410948282725 39.0 35.0 41.0 27.0 41.0 50-51 34.51191180117993 38.0 34.0 40.0 25.0 40.5 52-53 34.61156410809777 38.0 34.0 39.5 25.0 40.5 54-55 35.09812505263022 39.0 35.0 40.0 26.0 41.0 56-57 35.00378040301291 39.0 35.0 40.5 26.0 41.0 58-59 34.78401223828112 38.0 34.0 40.0 25.0 41.0 60-61 34.46437572388126 38.0 34.0 40.0 24.5 41.0 62-63 34.071674885801045 37.5 34.0 40.0 23.0 41.0 64-65 33.66907910569805 37.0 33.0 40.0 22.0 41.0 66-67 33.292934642811616 36.0 33.0 39.0 22.0 41.0 68-69 32.87789857356566 36.0 32.5 39.0 20.5 40.5 70-71 32.461101031161235 35.0 32.0 38.5 19.5 40.0 72-73 32.00902156792278 35.0 32.0 37.5 18.0 40.0 74-75 31.543933064019978 35.0 31.0 37.0 16.5 39.0 76-77 31.07178187182752 35.0 31.0 36.5 12.0 39.0 78-79 30.673624746397103 35.0 31.0 36.0 7.5 38.5 80-81 30.268906961730295 34.0 30.0 36.0 7.0 37.0 82-83 29.835178017845728 34.0 30.0 35.0 2.0 37.0 84-85 29.409520882061827 34.0 29.5 35.0 2.0 36.5 86-87 28.983678994064462 34.0 29.0 35.0 2.0 36.0 88-89 28.55184358780976 34.0 29.0 35.0 2.0 36.0 90-91 28.136669472078026 34.0 28.5 35.0 2.0 35.0 92-93 27.66927996333485 34.0 27.0 35.0 2.0 35.0 94-95 27.1731337610627 33.0 26.0 35.0 2.0 35.0 96-97 26.53761467636612 33.0 25.0 35.0 2.0 35.0 98-99 25.705178261727852 33.0 23.0 35.0 2.0 35.0 100-101 22.91988173097409 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2601345159314761 1101 2 0.36795138534290217 1101 3 0.41404864041457046 1101 4 0.2885840273702769 1101 5 0.2751123036520795 1101 6 0.34312954989267297 1101 7 0.2092257988157158 1101 8 0.27646376935862804 1101 9 0.21679467660757012 1101 10-11 0.511144837603112 1101 12-13 0.4502745128439045 1101 14-15 0.40373952810694647 1101 16-17 0.353760644147016 1101 18-19 0.5152699043876794 1101 20-21 0.584259911039382 1101 22-23 0.6151752272142801 1101 24-25 0.5417320707948576 1101 26-27 0.5967559541219245 1101 28-29 0.648154371211831 1101 30-31 0.5491463385084572 1101 32-33 0.5583533870978812 1101 34-35 0.45553484322515203 1101 36-37 0.29517342191493157 1101 38-39 0.7415257397366304 1101 40-41 0.5882189320508431 1101 42-43 0.31125634216492415 1101 44-45 0.3745064328974763 1101 46-47 0.360448670657469 1101 48-49 0.35137769462998136 1101 50-51 0.2797240562224488 1101 52-53 0.3956341744177436 1101 54-55 0.3857201161141788 1101 56-57 0.44850673382723016 1101 58-59 0.436760542282876 1101 60-61 0.3774798016619769 1101 62-63 0.43587121325045075 1101 64-65 0.34039775139574147 1101 66-67 0.3062068636535358 1101 68-69 0.2071419300621784 1101 70-71 0.34941844394508337 1101 72-73 0.20036696533901477 1101 74-75 0.2885356137361761 1101 76-77 0.40191195579335925 1101 78-79 0.423752623198812 1101 80-81 0.2632954374094254 1101 82-83 0.17409040669576825 1101 84-85 0.4195155420596244 1101 86-87 0.1741173410562169 1101 88-89 0.1372030742701682 1101 90-91 0.2600885142853997 1101 92-93 0.5031614964230187 1101 94-95 0.5829410867386571 1101 96-97 0.6806942315704312 1101 98-99 0.590872912986157 1101 100-101 0.05629741821211098 1102 1 0.37189934608188935 1102 2 0.4033155901764367 1102 3 0.48622732048893624 1102 4 0.35550244041743184 1102 5 0.3741880599474996 1102 6 0.4120886930395429 1102 7 0.47664805671667665 1102 8 0.4140155270060788 1102 9 0.5261195033778918 1102 10-11 0.5721655195365187 1102 12-13 0.5196963059823148 1102 14-15 0.8107046286646593 1102 16-17 0.7043986363728081 1102 18-19 0.6007011829071942 1102 20-21 0.7209645333141879 1102 22-23 0.6422751089110861 1102 24-25 0.6904566144825424 1102 26-27 0.6821555442858553 1102 28-29 0.6486186056211594 1102 30-31 0.7390160993669994 1102 32-33 0.6096800729184011 1102 34-35 0.5834654879775627 1102 36-37 0.5625592382069655 1102 38-39 0.9960239018120092 1102 40-41 0.7567011654461382 1102 42-43 0.7693458512066726 1102 44-45 0.7980588031864713 1102 46-47 0.7507509775697514 1102 48-49 0.6634060662710155 1102 50-51 0.7479353541774927 1102 52-53 0.7551934950193981 1102 54-55 0.7529311183112455 1102 56-57 0.6729981357200856 1102 58-59 0.7039034145154304 1102 60-61 0.7681143081686557 1102 62-63 0.7476703724527454 1102 64-65 0.7614895208735177 1102 66-67 0.7482699868578564 1102 68-69 0.6576896104745487 1102 70-71 0.6510308531051336 1102 72-73 0.6907094108350478 1102 74-75 0.6036477905149447 1102 76-77 0.6895402936334811 1102 78-79 0.6451243064842593 1102 80-81 0.5851852253086989 1102 82-83 0.6067420882911705 1102 84-85 0.5434201812348824 1102 86-87 0.5153623765471771 1102 88-89 0.5230901369708612 1102 90-91 0.7124857383854497 1102 92-93 0.5270661355982753 1102 94-95 0.5556325900342465 1102 96-97 0.3232136670951924 1102 98-99 0.4091956483250101 1102 100-101 0.010284341484037895 1103 1 0.42527682144206835 1103 2 0.4890294621238702 1103 3 0.4942944810552987 1103 4 0.41649141559969394 1103 5 0.3612317268320808 1103 6 0.49835158187693906 1103 7 0.4039228434198634 1103 8 0.44908459503762543 1103 9 0.5217284026753148 1103 10-11 0.5306260430757206 1103 12-13 0.553293398442662 1103 14-15 0.6219891885408586 1103 16-17 0.5787210931548614 1103 18-19 0.6645941548703576 1103 20-21 0.7023292501735341 1103 22-23 0.6519562187992847 1103 24-25 0.6575289192052836 1103 26-27 0.6170613554343873 1103 28-29 0.6892175383095633 1103 30-31 0.7106033762432205 1103 32-33 0.7822839490862563 1103 34-35 0.6433627651847971 1103 36-37 0.414407043380713 1103 38-39 0.8477850745513962 1103 40-41 0.6761362340486841 1103 42-43 0.7206169171114567 1103 44-45 0.8011740796654792 1103 46-47 0.6594422019886181 1103 48-49 0.633842554093853 1103 50-51 0.6483041756039967 1103 52-53 0.7890277276060189 1103 54-55 0.6678011748317942 1103 56-57 0.7121150072702989 1103 58-59 0.6599423675611646 1103 60-61 0.709477985438788 1103 62-63 0.5392243307533207 1103 64-65 0.5923881810964531 1103 66-67 0.6131518357336532 1103 68-69 0.4947589567643007 1103 70-71 0.44430504708730467 1103 72-73 0.5382050352505914 1103 74-75 0.3907381096119451 1103 76-77 0.4465010157807683 1103 78-79 0.5793692543910147 1103 80-81 0.49030551250427123 1103 82-83 0.47162445437552947 1103 84-85 0.4850689697255426 1103 86-87 0.3811055069830367 1103 88-89 0.3683074576649936 1103 90-91 0.5373161198371115 1103 92-93 0.5089723698234145 1103 94-95 0.36976787420931956 1103 96-97 0.45627539697163755 1103 98-99 0.6365991941000395 1103 100-101 0.19290693542980364 1104 1 0.22910227994006505 1104 2 0.31428175519895873 1104 3 0.3011607846337796 1104 4 0.26853753750597065 1104 5 0.24233820716045074 1104 6 0.2253200073952044 1104 7 0.24554021922698155 1104 8 0.25001234472603784 1104 9 0.4085721592116087 1104 10-11 0.36061402800526565 1104 12-13 0.38698087702456974 1104 14-15 0.33960867222049274 1104 16-17 0.36018834586724324 1104 18-19 0.47767198133745836 1104 20-21 0.5719111666179231 1104 22-23 0.5752162077277845 1104 24-25 0.568811284693524 1104 26-27 0.42448899462948475 1104 28-29 0.4277937980380955 1104 30-31 0.46214085934587246 1104 32-33 0.4209764670010756 1104 34-35 0.3140856575524893 1104 36-37 0.2524755758446844 1104 38-39 0.5859646916015109 1104 40-41 0.46611171857481537 1104 42-43 0.25912882710855456 1104 44-45 0.4428076660864022 1104 46-47 0.4095312002293454 1104 48-49 0.44053307844808387 1104 50-51 0.29861056359591487 1104 52-53 0.3942602328923286 1104 54-55 0.5378166882413993 1104 56-57 0.5631393431463394 1104 58-59 0.5972841570207734 1104 60-61 0.5843092583242608 1104 62-63 0.5816286357799214 1104 64-65 0.4812938636556865 1104 66-67 0.5868472816598 1104 68-69 0.4919609013481434 1104 70-71 0.6651199679472413 1104 72-73 0.5635189680760817 1104 74-75 0.5733203717020992 1104 76-77 0.6835373903779924 1104 78-79 0.6306547831662073 1104 80-81 0.5553422241245975 1104 82-83 0.4250841023884142 1104 84-85 0.45680780934615584 1104 86-87 0.33923950273428005 1104 88-89 0.4425112960328583 1104 90-91 0.4940096374885705 1104 92-93 0.5376963210545966 1104 94-95 0.5270449493218656 1104 96-97 0.5862813170483854 1104 98-99 0.4281061874524532 1104 100-101 0.10200628801533185 1105 1 0.1584144625280075 1105 2 0.3605481791272247 1105 3 0.3512353216701527 1105 4 0.3827588333811818 1105 5 0.30931913166681824 1105 6 0.23031472541140374 1105 7 0.31442460627212654 1105 8 0.35643445768113224 1105 9 0.4210885362126646 1105 10-11 0.4156324353505241 1105 12-13 0.42586250308672646 1105 14-15 0.5068363269103884 1105 16-17 0.5102752146606733 1105 18-19 0.3857604734558251 1105 20-21 0.5411978445661205 1105 22-23 0.5578676352890426 1105 24-25 0.5173639783626953 1105 26-27 0.5206361878122792 1105 28-29 0.5423163784594465 1105 30-31 0.6229997198915243 1105 32-33 0.6204527356157357 1105 34-35 0.6218424753033744 1105 36-37 0.5765281333655636 1105 38-39 1.1124672769935842 1105 40-41 0.8529874501046208 1105 42-43 0.6055902543704335 1105 44-45 0.5297426328191719 1105 46-47 0.6587768008334365 1105 48-49 0.6943689914709452 1105 50-51 0.510254878655914 1105 52-53 0.6110275732673571 1105 54-55 0.822215162239921 1105 56-57 0.6975177156680417 1105 58-59 0.7325966779598119 1105 60-61 0.8577262907855356 1105 62-63 0.672971314636726 1105 64-65 0.6685261706731893 1105 66-67 0.5373989555235639 1105 68-69 0.6555139775631602 1105 70-71 0.6479094446577989 1105 72-73 0.711520962066551 1105 74-75 0.536924224342588 1105 76-77 0.5801154109575322 1105 78-79 0.5359076839803869 1105 80-81 0.6068320619489249 1105 82-83 0.4068522292396608 1105 84-85 0.5337743681871707 1105 86-87 0.602573007144013 1105 88-89 0.5394132450207287 1105 90-91 0.45178964559569224 1105 92-93 0.5763297675359595 1105 94-95 0.7589835585562845 1105 96-97 0.7575590866683548 1105 98-99 0.8941722333969189 1105 100-101 0.6428278491539992 1106 1 0.13258964240991133 1106 2 0.18798713723456828 1106 3 0.2123981763746059 1106 4 0.19397129871734364 1106 5 0.13957467845924043 1106 6 0.14698389766552822 1106 7 0.11521791380947377 1106 8 0.07367952957607571 1106 9 0.18972975998784136 1106 10-11 0.2759524875284214 1106 12-13 0.27000416977266894 1106 14-15 0.3965867010138666 1106 16-17 0.38095718160079883 1106 18-19 0.22897561244654696 1106 20-21 0.24228939410620853 1106 22-23 0.34272193730561895 1106 24-25 0.43283266584347047 1106 26-27 0.4725873080411631 1106 28-29 0.3544299301412366 1106 30-31 0.26030043895657684 1106 32-33 0.2408869963248108 1106 34-35 0.14581204282355742 1106 36-37 0.15473888950560166 1106 38-39 0.49222914596137457 1106 40-41 0.5072513046541118 1106 42-43 0.35080381137177596 1106 44-45 0.2404900996702537 1106 46-47 0.184897087534317 1106 48-49 0.1885351743664856 1106 50-51 0.17344910948297354 1106 52-53 0.34133354018897677 1106 54-55 0.16300909424675325 1106 56-57 0.2237320197199324 1106 58-59 0.21027108419340124 1106 60-61 0.3066390200178759 1106 62-63 0.2014006350982811 1106 64-65 0.17922344868006945 1106 66-67 -0.043627232876573885 1106 68-69 0.1453603349401078 1106 70-71 0.1738968581282947 1106 72-73 0.010006507910702567 1106 74-75 0.18116545921943938 1106 76-77 0.3576457266384274 1106 78-79 0.6099716157790702 1106 80-81 0.5508355358495685 1106 82-83 0.39813845136114523 1106 84-85 0.4813210125888041 1106 86-87 0.4057167484563706 1106 88-89 0.3780979735037029 1106 90-91 0.3372103645682323 1106 92-93 0.32019262015884564 1106 94-95 0.5627700408733887 1106 96-97 0.42319857811890316 1106 98-99 0.4481328733162506 1106 100-101 0.30380764178386954 1107 1 0.09581490924664138 1107 2 -0.03473931725061519 1107 3 0.06300056220888095 1107 4 0.09711538396459218 1107 5 0.05070827903176678 1107 6 0.05293575070446366 1107 7 0.13058467984931355 1107 8 0.06313316738061303 1107 9 0.15963439686596814 1107 10-11 0.18625155102041902 1107 12-13 0.20946044191759228 1107 14-15 0.2054445607879316 1107 16-17 0.24946924029389805 1107 18-19 0.31959275058696335 1107 20-21 0.28103373576259827 1107 22-23 0.09574242808323419 1107 24-25 0.20029296413861886 1107 26-27 0.1946764716819942 1107 28-29 0.3525977464047685 1107 30-31 0.2501696439565606 1107 32-33 0.27918251223841395 1107 34-35 0.42501337667790295 1107 36-37 0.236020364384963 1107 38-39 0.6453617059694636 1107 40-41 0.4807990147537353 1107 42-43 0.33677704647348605 1107 44-45 0.3649341440852325 1107 46-47 0.5037366825483929 1107 48-49 0.3121671833583761 1107 50-51 0.3334999961580394 1107 52-53 0.4788363391756363 1107 54-55 0.5246709898302839 1107 56-57 0.4821795809793201 1107 58-59 0.4886585166335422 1107 60-61 0.506105675854478 1107 62-63 0.4421696868674587 1107 64-65 0.4472035901441487 1107 66-67 0.4310996738370463 1107 68-69 0.4998511169440505 1107 70-71 0.48533274925978986 1107 72-73 0.36685085351859925 1107 74-75 0.4224761972299014 1107 76-77 0.4236662481500133 1107 78-79 0.609147177003333 1107 80-81 0.5121273066092691 1107 82-83 0.38681693450795507 1107 84-85 0.23294190027369766 1107 86-87 0.6065229663777849 1107 88-89 0.5196101287892922 1107 90-91 0.4064140473760851 1107 92-93 0.3152477437313621 1107 94-95 0.3691169352056036 1107 96-97 0.4744534022547313 1107 98-99 0.4590175287225726 1107 100-101 0.20713206958318864 1108 1 -0.009634683520069132 1108 2 0.030651265075789524 1108 3 0.07359630503310655 1108 4 0.022329950221497086 1108 5 0.16727130894476971 1108 6 0.06407708628868392 1108 7 0.10384560030477985 1108 8 0.11156632398189004 1108 9 0.13096256388406147 1108 10-11 0.18882124886984997 1108 12-13 0.21198136994129868 1108 14-15 0.1702743845117567 1108 16-17 0.3016207552420056 1108 18-19 0.25047447547396473 1108 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-1.0778656538291997 2316 92-93 -1.2182756247013486 2316 94-95 -1.18264928354591 2316 96-97 -1.0767809605219263 2316 98-99 -1.2672055842531194 2316 100-101 -0.4824289804905426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9153.0 3 2424.0 4 2415.0 5 2741.0 6 3088.0 7 3596.0 8 4544.0 9 5615.0 10 6217.0 11 7610.0 12 9264.0 13 11471.0 14 11682.0 15 11916.0 16 14092.0 17 17005.0 18 15825.0 19 12780.0 20 13006.0 21 14039.0 22 15383.0 23 17133.0 24 19479.0 25 22065.0 26 25581.0 27 29922.0 28 34779.0 29 41195.0 30 49640.0 31 60111.0 32 74751.0 33 96385.0 34 131246.0 35 187973.0 36 294920.0 37 454295.0 38 388447.0 39 51380.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00777918486548 18.629938600928185 14.67633495907344 23.6859472551329 2 15.414601943502074 19.98670463498368 39.603591592571746 24.9951018289425 3 19.308564070908325 23.62164831015777 29.460525630345657 27.609261988588248 4 13.05317344749008 16.466624921497257 36.79083549052572 33.68936614048694 5 14.423642739479398 36.74320193763916 33.870546741117465 14.96260858176398 6 32.54759570735734 35.43125433023276 17.49391003434704 14.527239928062857 7 29.137496273791868 32.046034968994725 20.44500715754477 18.37146159966864 8 26.621680031482043 34.52329496075343 19.848663165180273 19.006361842584255 9 26.425433240462624 16.87626322439842 20.139471954920687 36.55883158021827 10-11 25.26085288774877 26.338544900378167 27.04470397968975 21.355898232183318 12-13 25.92279908042588 23.463053483647155 27.57059205646443 23.04355537946254 14-15 22.96378349535947 24.97458790006202 25.72946319876363 26.332165405814877 16-17 22.710084851382824 28.152214245883595 26.324612336813857 22.813088565919728 18-19 22.714523223868714 26.88653901272638 28.289606467422807 22.1093312959821 20-21 23.421986056687256 26.119155842135577 28.195949382608216 22.26290871856895 22-23 23.10488109606923 25.910185546044488 28.16809609204865 22.816837265837627 24-25 22.89825150902093 26.12295961743807 28.220443383821525 22.758345489719478 26-27 22.659002996031795 26.348116353622565 28.223569106138903 22.769311544206737 28-29 22.6216163269069 26.584117911428145 28.236629521647444 22.557636240017516 30-31 22.775366872759292 26.267211545796094 28.054891663676564 22.902529917768053 32-33 22.61068774422981 26.297870771244753 28.104021876537686 22.987419607987746 34-35 23.15384382711246 26.39155414851911 27.842674011661693 22.611928012706738 36-37 23.045385455617268 26.19084662108664 27.967242485363723 22.79652543793237 38-39 22.780006563105182 26.475589408442374 27.968025660816874 22.776378367635573 40-41 23.01449851363951 26.29572161559818 27.90725295394651 22.782526916815804 42-43 22.801558902370758 26.273010381742484 27.734932051104856 23.190498664781906 44-45 22.933991795115023 26.424846993387412 27.670672956354252 22.97048825514331 46-47 22.96734991440719 26.65780304546751 27.411519040066125 22.96332800005917 48-49 23.08460867349982 26.48848506648752 27.407626935665153 23.019279324347504 50-51 23.005063277094216 26.701112238133064 27.365690472513972 22.928134012258752 52-53 23.160676584086744 26.72689305927774 27.057237419578257 23.05519293705726 54-55 22.903905852202637 26.617734786466567 27.25935321811167 23.219006143219126 56-57 22.883430224901495 26.834628481176726 27.277537159737776 23.004404134184 58-59 23.116188983595894 26.57209878687967 27.23156201255258 23.080150216971855 60-61 23.017573691694466 26.641255710999523 27.480500743763447 22.86066985354256 62-63 22.873379933248604 26.623534236328055 27.494612684244814 23.00847314617853 64-65 22.669079060211363 26.529486633592185 27.430458756993193 23.37097554920326 66-67 22.748225159105132 26.653071632391264 27.257172937204764 23.34153027129884 68-69 22.753742144617647 26.515609032013426 27.50907501861246 23.221573804756463 70-71 22.77783018126366 26.46501651172189 27.395540586653944 23.36161272036051 72-73 22.853771638440364 26.473353518153047 27.286309962357475 23.386564881049118 74-75 22.700441586790266 26.76668815918116 27.108980919594494 23.423889334434083 76-77 22.83986214391589 26.459659113365618 27.18703000849871 23.513448734219786 78-79 22.80961558904958 26.58261576930721 27.22412494164737 23.38364369999584 80-81 22.8124753742365 26.643660666973712 27.120057255405648 23.423806703384138 82-83 22.913286498649782 26.551734020414596 27.103539226589824 23.431440254345798 84-85 22.79309558995855 26.454308738495076 27.138906852591372 23.613688818955 86-87 22.722495663398856 26.50183922737427 27.20198218208755 23.573682927139323 88-89 22.713250576940148 26.404835387141635 27.129986279179857 23.751927756738365 90-91 22.724753824838277 26.407792712803698 27.19633002325524 23.671123439102786 92-93 22.70928861764652 26.228110391851818 27.282376667607643 23.780224322894018 94-95 22.599000676018722 26.180258403763514 27.3351314259926 23.88560949422517 96-97 22.61374809758051 26.098293966379625 27.377755603915745 23.910202332124122 98-99 22.53184023140475 26.038721734721882 27.139706781586103 24.289731252287265 100-101 22.678989054454057 25.47632795124547 27.602242641909093 24.24244035239138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2313.0 1 1336.5 2 358.0 3 368.0 4 330.5 5 311.0 6 442.0 7 791.5 8 1272.0 9 1561.5 10 1540.5 11 1556.0 12 1722.5 13 1928.5 14 2112.5 15 2238.0 16 2363.0 17 2470.0 18 2574.0 19 2745.0 20 3002.0 21 3374.5 22 3915.0 23 4799.5 24 5891.5 25 7171.0 26 9201.5 27 11730.5 28 14301.0 29 17261.5 30 20682.5 31 24543.5 32 28795.0 33 32170.5 34 35672.5 35 39747.5 36 44452.0 37 49266.0 38 53197.5 39 56303.0 40 59149.5 41 63546.5 42 68498.5 43 72767.0 44 76767.5 45 80173.5 46 81101.0 47 83357.0 48 89469.5 49 91320.5 50 88383.0 51 85484.0 52 82952.5 53 82157.0 54 81306.0 55 75675.5 56 67094.5 57 60682.0 58 55296.5 59 47984.0 60 39893.0 61 32927.5 62 26717.0 63 20717.5 64 15694.5 65 11959.5 66 9100.5 67 7184.5 68 5406.0 69 3828.5 70 2939.0 71 2308.5 72 1815.5 73 1427.0 74 1056.0 75 790.5 76 554.0 77 317.5 78 197.5 79 154.0 80 100.5 81 65.0 82 45.5 83 27.5 84 26.0 85 26.0 86 15.0 87 10.5 88 7.5 89 9.5 90 7.0 91 5.5 92 7.0 93 5.0 94 3.5 95 4.0 96 3.0 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2932567512784255 2 0.18424604301252404 3 0.18470619796510276 4 0.20555121731691933 5 0.219447896884797 6 0.1102991421331219 7 0.12626651898760388 8 0.14057733801280242 9 0.14444263961446377 10-11 0.13107513824205164 12-13 0.12014645811830675 14-15 0.1359757884870151 16-17 0.10976996393765635 18-19 0.14002515206970795 20-21 0.16613894562855097 22-23 0.17115463461165914 24-25 0.194415467464514 26-27 0.17538806017538347 28-29 0.1728342001885715 30-31 0.16222762853163175 32-33 0.17179885154526936 34-35 0.35503255366212016 36-37 0.47481088781836395 38-39 0.4404143051131038 40-41 0.4297157024656483 42-43 0.4538968452236606 44-45 0.45819929403027176 46-47 0.4616274484269833 48-49 0.4736835081845461 50-51 0.46728735434370167 52-53 0.47333839197011196 54-55 0.4556224262958309 56-57 0.41772866595097236 58-59 0.4070070555558879 60-61 0.3608535138122411 62-63 0.47073851648804216 64-65 0.46862180370618006 66-67 0.47770986401960996 68-69 0.49011103999160677 70-71 0.4820123128262211 72-73 0.47554713574248997 74-75 0.479136344372604 76-77 0.4560365657531517 78-79 0.4422549249234188 80-81 0.4391028634982544 82-83 0.4521942718991193 84-85 0.45408090720469213 86-87 0.45594453476263597 88-89 0.46222564986533565 90-91 0.48051680923034024 92-93 0.4849803122703539 94-95 0.4840369946175675 96-97 0.48198930507859217 98-99 0.4781240034769308 100-101 0.46098323149337306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.81671002436265 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37996179511298 40.772647973458795 2 14.058300138134907 15.41259524215197 3 4.916320570516404 8.084895573024205 4 2.284381514981641 5.008891163670514 5 1.231628283498952 3.3756905237182786 6 0.7164556933353453 2.356424639212039 7 0.4909931252793567 1.884023944067584 8 0.32892538025170215 1.442448575112862 9 0.22511476237460876 1.1106045586163007 >10 1.2411465119103622 12.336313458607941 >50 0.08516153355707719 3.190378507940698 >100 0.03959073227189456 3.800762707381623 >500 0.0015149690810394413 0.5279394660792823 >1k 4.2082474473313777E-4 0.4651477494547674 >5k 8.416494894662756E-5 0.2312359175032032 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5012 0.23062966223246018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3772 0.17357044811269748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2505 0.1152688156209722 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.203099051574627E-5 2 0.0 0.0 0.0 0.0 9.203099051574627E-5 3 0.0 0.0 0.0 0.0 9.203099051574627E-5 4 0.0 0.0 0.0 0.0 9.203099051574627E-5 5 0.0 0.0 0.0 0.0 9.203099051574627E-5 6 0.0 0.0 0.0 0.0 9.203099051574627E-5 7 0.0 0.0 0.0 0.0 9.203099051574627E-5 8 0.0 0.0 0.0 0.0 9.203099051574627E-5 9 0.0 0.0 0.0 0.0 9.203099051574627E-5 10-11 0.0 0.0 0.0 2.3007747628936568E-5 9.203099051574627E-5 12-13 0.0 0.0 0.0 4.6015495257873136E-5 2.5308522391830226E-4 14-15 0.0 0.0 0.0 9.203099051574627E-5 2.991007191761754E-4 16-17 0.0 0.0 0.0 9.203099051574627E-5 3.2210846680511197E-4 18-19 0.0 0.0 0.0 9.203099051574627E-5 4.141394573208582E-4 20-21 0.0 0.0 0.0 1.3804648577361942E-4 4.141394573208582E-4 22-23 0.0 0.0 0.0 2.300774762893657E-4 4.141394573208582E-4 24-25 0.0 0.0 0.0 2.7609297154723884E-4 4.141394573208582E-4 26-27 0.0 0.0 0.0 5.29178195465541E-4 4.371472049497948E-4 28-29 0.0 0.0 0.0 0.0017485888197991792 4.601549525787314E-4 30-31 0.0 0.0 0.0 0.004325456554240075 4.601549525787314E-4 32-33 0.0 0.0 0.0 0.010813641385600186 4.83162700207668E-4 34-35 0.0 0.0 0.0 0.0215812672759425 5.061704478366045E-4 36-37 0.0 0.0 0.0 0.04162101546074625 5.061704478366045E-4 38-39 0.0 0.0 0.0 0.07470615655115703 5.521859430944777E-4 40-41 0.0 0.0 0.0 0.11453256769684624 5.521859430944777E-4 42-43 0.0 0.0 0.0 0.15677479234357378 5.521859430944777E-4 44-45 0.0 0.0 0.0 0.20962358864724107 5.982014383523508E-4 46-47 0.0 0.0 0.0 0.2745054369608422 6.442169336102239E-4 48-49 0.0 0.0 0.0 0.3454843383961115 6.442169336102239E-4 50-51 0.0 0.0 0.0 0.4242168507823324 6.90232428868097E-4 52-53 0.0 0.0 0.0 0.5044218590168053 7.362479241259702E-4 54-55 0.0 0.0 0.0 0.5977412833997721 7.822634193838434E-4 56-57 0.0 0.0 0.0 0.7108013552483663 7.822634193838434E-4 58-59 0.0 0.0 0.0 0.8370908819835992 7.822634193838434E-4 60-61 0.0 0.0 0.0 0.9733427634421614 7.822634193838434E-4 62-63 0.0 0.0 0.0 1.1155536515366185 8.742944098995896E-4 64-65 0.0 0.0 0.0 1.2712240719940033 9.203099051574628E-4 66-67 0.0 0.0 0.0 1.452663169795797 9.203099051574628E-4 68-69 0.0 0.0 0.0 1.6608142625947862 9.203099051574628E-4 70-71 0.0 0.0 0.0 1.89266333545158 9.433176527863993E-4 72-73 0.0 2.3007747628936568E-5 0.0 2.1289759113483875 9.893331480442724E-4 74-75 0.0 4.6015495257873136E-5 0.0 2.3747906870159454 0.001012340895673209 76-77 0.0 4.6015495257873136E-5 0.0 2.649871317667511 0.001012340895673209 78-79 0.0 4.6015495257873136E-5 0.0 2.96123516632991 0.001012340895673209 80-81 0.0 4.6015495257873136E-5 0.0 3.304717830682304 0.001012340895673209 82-83 0.0 4.6015495257873136E-5 0.0 3.661637019649997 0.001058356390931082 84-85 0.0 4.6015495257873136E-5 0.0 4.038158809597544 0.001058356390931082 86-87 0.0 4.6015495257873136E-5 0.0 4.433592968096077 0.0010813641385600187 88-89 0.0 4.6015495257873136E-5 0.0 4.86643772423926 0.0011043718861889554 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5685 0.0 30.636448 1 GTATCAA 8355 0.0 29.820555 1 ATCAACG 9975 0.0 24.573086 3 TCAACGC 10100 0.0 24.315907 4 TATCAAC 10210 0.0 24.239677 2 CAACGCA 10135 0.0 24.13782 5 AACGCAG 10545 0.0 23.322367 6 GTACATG 7745 0.0 22.978025 1 TACATGG 7715 0.0 22.614864 2 GTATAGG 550 0.0 21.571493 1 ACATGGG 7915 0.0 21.204811 3 ACGCAGA 11795 0.0 20.649857 7 TATCACG 115 0.0037648636 20.613619 2 CGCAGAG 11845 0.0 20.523155 8 GAGTACT 5295 0.0 20.492092 12-13 TAGACCG 140 4.9159536E-4 20.31867 5 GTGCGAA 120 0.0047896984 19.773867 1 CTAGACG 120 0.0048166174 19.754719 4 AGAGTAC 9730 0.0 19.477982 10-11 GTACTTT 5820 0.0 18.56623 14-15 >>END_MODULE